Method for predicting respiratory distress syndrome outcome in newborns having perinatal central nervous system injury |
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IPC classes for russian patent Method for predicting respiratory distress syndrome outcome in newborns having perinatal central nervous system injury (RU 2308724):
 Method for determining tuberculous spondilitis activity degree / 2308723
Method involves studying intracellular lysosomal cation proteins availability in blood granulocytes by means of cytochemical lysosomal cation test and myeloperoxidase and lactoferrin serum proteins concentration in parallel to it. Active tuberculous spondilitis diagnosis is set when having lysosomal cathione test value ≥ 1.6 mean cytochemical coefficient, myeloperoxidase > 200 ng/ml and lactoferrine > 1300 ng/ml.
 Method for evaluating functional liver condition / 2305844
Method involves determining α-globulin, albumin, β-globulin concentrations and calculating diagnostic criterion value as newborn cattle calves functional liver state coefficient from formula of Kfsl=A/(A+B+C), where A is the α-globulins quantity, B is the albumins quantity and C is the β-globulins quantity. Coefficient Kfsl value being <0.2, functional hepatic insufficiency is diagnosed.
 Method for selecting chronic cardiac insufficiency patients treatable with nebivolol / 2305498
Method involves determining endothelin-1 concentration in blood plasma. Its value being equal to 1.5-5 fmole/ml, treatment with Nebivolol is administered.
Method for differential diagnostics of purulent meningitis / 2305287
In spinal fluid (SF) one should detect the concentration of C-reactive protein (C-RP) and at its value being >2.9 mg/l it is possible to diagnose meningococcal meningitis, at SF C-RP value being ≤2.9 mg/l - nonmeningococcal meningitis. Application of the present method provides the chance to increase accuracy, specificity and information value of differential diagnostics of purulent meningococcal and non-meningococcal meningitis that enables to carry out earlier specific treatment. The method in question, moreover, is very quick and simple in implementation.
Method for evaluating the severity of tuberculosis infection / 2305286
In the experiment upon guinea pigs in blood plasma one should detect the concentration of taurine and glutamic acid and at taurine concentration being below 68 mcM/l and glutamic acid being below 76 mcM/l it is necessary to evaluate the state as severe, at taurine concentration ranged 68-89 mcM/l and that of glutamic acid ranged 125-227 mcM/l - as average severity degree, and at taurine and glutamic acid concentrations being above 89 and 227 mcM/l, correspondingly - as light severity degree. Application of the present method enables to increase information value and significance of the method in question and, also, detect body reactivity according to the state of its different systems.
 Method for predicting diabetic retinopathy / 2304786
Due to biochemical testing lacrimal fluid (LF0 it is necessary to detect the concentration of cholesterol, activity of superoxide dismutase (SOD) and catalase, in blood one should detect the concentration of nitrites and based upon the values obtained calculate Function 1 and Function 2. Function 1 = -3.4+0.29(NO2)+32.5(cholesterol)-0.97(catalase)-17.28(SOD) - corresponds to Y-axis values and Function 2 = -7.14+0.17(NO2)+37.2(cholesterol)+2.1(catalase)-17.28(SOD) - corresponds to X-axis values on the scheme to detect lesion type. If the point obtained is on the field of the 1st scheme one should detect the presence of nonproliferative DR form in a patient at no risk of proliferation; if it is on the field of the 2nd scheme - nonproliferative DR form with the risk of proliferation; if it is on the field of the 3d scheme - proliferative form of retinopathy in the phase of neovascularization. Application of the present method enables to carry out diagnostics in due time, differentiation in doubtful cases directed towards adequate treatment and decreasing the risk for DR progressing.
Method for predicting the development of postgastroresectional peptic ulcers / 2304785
The present innovation deals with predicting the development of postgastroresectional peptic ulcers in patients due to detecting the level of gastrin after stimulation, pepsinogen, prostaglandins PGE2 and PG F2α, correspondingly, in mucosa of gastric stump and in mucosa of gastroenteroanastomosis. At increasing gastrin level up to 12.5 pM/l and higher, that of pepsinogen up to 10.0 mcg/l and higher and decreasing the level of prostaglandin PGE2 up to 570 and lower and at the decrease of prostaglandin PG F2α level up to 652 ng/g and lower one should predict the development of postgastroresectional peptic ulcer. Application of the present innovation enables to increase the accuracy in predicting the development of postgastroresectional peptic ulcers.
Method for predicting the chance for osteomyelitis chronization in children / 2304784
During the moment of exacerbation and on ending the disease in question one should detect the content of alpha-1 proteinase inhibitor and alpha-1 acid glycoprotein in blood due to immunoturbidimetric technique. At decreased content of alpha-1 proteinase inhibitor from 2.3-2.6 g/l up to 1-1.2 g/l, and alpha-1 acid glycoprotein from 2.4-2.6 g/l up to 0.7-0.8 g/l one should predict the absence of osteomyelitis chronization. At decreased content of alpha-1 proteinase inhibitor from 2.9-3.1 g/l up to 1.8-1.6 g/l, and that of alpha-1 acid glycoprotein from 3-3.2 g/l up to 1.2-1.3 g/l one should predict osteomyelitis chronization. Application of the present method enables to predict the disease flow in shorter terms at high significance that provides the chance to prescribe the complex of efficient measures in due time which vary depending upon the disease flow.
Method for predicting the development of gestosis / 2304783
Starting from 22nd wk of pregnancy it is necessary to detect the concentration of neurospecific enolase and glyofibrous acid protein in blood serum. The development of gestosis in pregnant women should be predicted by the value of neurospecific enolase being above 12.4 ng/ml and glyofibrous acid protein being above 4 ng/ml. Moreover, severe gestosis should be predicted at the value of neurospecific enolase being 19 ng/ml and higher and glyofibrous acid protein being 10 ng/ml and higher. Application of the method enables to increase the accuracy of prediction.
Method for evaluating the results of surgical sanitation of inflammation focus in patients with chronic osteomyelitis / 2304781
The present innovation deals with laboratory investigations in case of traumatological pathology. One should simultaneously carry out microscopic investigation of material sample out of inflammation focus and blood from cubital vein to study the growth of microorganisms in the focus and in case of its absence and at registering positive index of digestion in blood with test culture S.aureus 209 P inside neutrophil after incubation, the value of IgM being 0.5-1.3 against the norm, the value of C-reactive protein being up to 12.0 mg/l it is possible to conclude upon satisfactory result of therapy conducted. Application of the present method enables to evaluate the degree of inflammatory process in bony tissue and, also, the state of phagocytic function of neutrophils as the first line of protection against microbial invasion.
Method for determining tuberculous spondilitis activity degree / 2308723
Method involves studying intracellular lysosomal cation proteins availability in blood granulocytes by means of cytochemical lysosomal cation test and myeloperoxidase and lactoferrin serum proteins concentration in parallel to it. Active tuberculous spondilitis diagnosis is set when having lysosomal cathione test value ≥ 1.6 mean cytochemical coefficient, myeloperoxidase > 200 ng/ml and lactoferrine > 1300 ng/ml.
Method of diagnosing small abnormalities in fetus heart development / 2308034
Invention resides in that peripheral venous blood of women at 36-38 weak pregnancy term is assayed to determine amount of CD16+ lymphocytes and antibody/collagen ratio. When value of CD16+ lymphocytes is 14.0% or less and antibody/collagen ratio 0.1 or higher, small abnormalities in fetus heart development are diagnosed. Method is simple to perform and allows diagnosing independent of dimensions, position, and degree of mobility of fetus.
Method for differential diagnostics of viral gastro-intestinal infections in cattle due to immunoenzymatic assay / 2306567
The present innovation deals with interaction of antibodies with an antigen, with antibodies labeled with horseradish peroxidase, addition of substrate mixture and registration of reaction results. Moreover, one should apply plotting boards with presorbed antibodies and general immunoenzymatic conjugate. The innovation enables to shorten terms for diagnosis and obtain more significant results of diagnostics.
 Method for predicting rapid disease progress in hiv-infected patients / 2306566
In patients in the stages of generalized lymphoadenopathy (GLAP) or pre-AIDS it is necessary to sample venous blood to detect quantitative content of CD+-, CD8+-, CD16+-, CD20+-lymphocytes and circulating immune complexes (CIC). In case of detecting during GLAP stage the quantity of CD4+- being below 0.46x10*9/l, CD20+ - above 0.095x10*9/l and CIC being above 170 c.u. at probability of 99.0% one should predict rapid disease progress. In case of detecting during pre-AIDS stage the quantity of CD8+ - above 0.11x10*9/l at probability of 95.5% it is, also, possible to predict rapid disease progress. The innovation enables to develop simple and available method for predicting rapid flow of HIV-infection.
Method for evaluation of paratonsillitis treatment effectiveness using immunoglobulin preparation for intravenous administration in patient having basic low level of immunoglobulin g in saliva / 2305843
Claimed method includes determination of immunoglobulin G (IgG) basic level in saliva, and when content thereof is less than 11.95 mug/ml IgG content is determined again in 5-10 days after preparation administering. When its value is more than 35 mum/ml treatment is evaluated as effective one, and when its value is less than 15 mug/ml treatment is evaluated as ineffective one with possible recrudescence.
 Method for predicting repeated respiratory diseases occurrence in early age children subjected to frequent and prolonged acute respiratory diseases / 2305838
Method involves carrying out large intestine and oropharyngeal mucous membrane micro-flora studies by applying standard microbiological methods with anaerobic and aerobic micro-organisms being isolated. Adeno- and rheovirus antigen titres are determined in smears taken from oropharynx. Adeno-, rheo- and rotavirus antigens are determined in feces material in 3 weeks or later after acute broncopulmonary disease. Adeno-, rheo- and rotavirus antigens in feces and adeno- and rheovirus antigen in smears taken from oropharynx being determined in titres≥108 in combination with intestinal dysbiosis of III degree and dysbiotic changes in oropharyngeal mucous membrane micro-flora being determined, child predisposition to respiratory diseases with high frequency, is to be predicted.
 Method for matching laboratory methods for predicting the development of post-traumatic purulent-inflammatory complications in the field of orthopedics and traumatology / 2305285
For matching laboratory methods it is necessary to detect human body state: the state with traumatic aftereffects that require planned surgical therapy, at inborn diseases and degenerative-dystrophic diseases of motor system being clinically healthy should be detected as steady state; the state with lesions should be detected as non steady one. In case of steady state one should match methods for detecting serumal factors of inspecific resistance, and in case of non steady state - for detecting cellular factors of adaptive immune response. In case of non steady body state laboratory techniques should be applied while testing blood out of elbow vein and additionally out of the vein coming from lesion site, or additionally it is necessary to conduct trials at loading tests. Based upon the data obtained it is important to predict post-traumatic purulent-inflammatory complications. Application of the present method enables to optimize the scope of laboratory trials and, correspondingly, increase prediction quality.
Method for predicting the chance for osteomyelitis chronization in children / 2304784
During the moment of exacerbation and on ending the disease in question one should detect the content of alpha-1 proteinase inhibitor and alpha-1 acid glycoprotein in blood due to immunoturbidimetric technique. At decreased content of alpha-1 proteinase inhibitor from 2.3-2.6 g/l up to 1-1.2 g/l, and alpha-1 acid glycoprotein from 2.4-2.6 g/l up to 0.7-0.8 g/l one should predict the absence of osteomyelitis chronization. At decreased content of alpha-1 proteinase inhibitor from 2.9-3.1 g/l up to 1.8-1.6 g/l, and that of alpha-1 acid glycoprotein from 3-3.2 g/l up to 1.2-1.3 g/l one should predict osteomyelitis chronization. Application of the present method enables to predict the disease flow in shorter terms at high significance that provides the chance to prescribe the complex of efficient measures in due time which vary depending upon the disease flow.
Method for predicting the development of gestosis / 2304783
Starting from 22nd wk of pregnancy it is necessary to detect the concentration of neurospecific enolase and glyofibrous acid protein in blood serum. The development of gestosis in pregnant women should be predicted by the value of neurospecific enolase being above 12.4 ng/ml and glyofibrous acid protein being above 4 ng/ml. Moreover, severe gestosis should be predicted at the value of neurospecific enolase being 19 ng/ml and higher and glyofibrous acid protein being 10 ng/ml and higher. Application of the method enables to increase the accuracy of prediction.
Method for evaluating the results of surgical sanitation of inflammation focus in patients with chronic osteomyelitis / 2304781
The present innovation deals with laboratory investigations in case of traumatological pathology. One should simultaneously carry out microscopic investigation of material sample out of inflammation focus and blood from cubital vein to study the growth of microorganisms in the focus and in case of its absence and at registering positive index of digestion in blood with test culture S.aureus 209 P inside neutrophil after incubation, the value of IgM being 0.5-1.3 against the norm, the value of C-reactive protein being up to 12.0 mg/l it is possible to conclude upon satisfactory result of therapy conducted. Application of the present method enables to evaluate the degree of inflammatory process in bony tissue and, also, the state of phagocytic function of neutrophils as the first line of protection against microbial invasion.
 Method for predicting the character of bacterial keratitis flow / 2245553
In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula:
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FIELD: medicine. SUBSTANCE: method involves determining granulocytic colony-stimulating factor level in plasma. Unfavorable outcome is predicted when determining granulocitic colony-stimulating factor level in plasma of 1.47 ng/ml and lower. EFFECT: high accuracy of prognosis.
The invention relates to medicine, namely to neonatology, and can be used for early prediction of outcome in respiratory distress syndrome and perinatal lesions of the Central nervous system (CNS) in infants, which is on artificial lung ventilation (ALV). Respiratory distress syndrome continues to be a pressing problem of neonatology, occupying a leading place in the structure of morbidity and mortality in newborns (Kassil C. L., Zolotokrylin Essary respiratory distress syndrome in the light of modern views, " Vestn. intens. Ter. - 2001. No. 31; - P.9-14., Antonov A.G., Volodin N.N., Grebennikov, VA and other Principles of newborns with respiratory distress syndrome: Method. recommendations. - M.: B. I., 2002. - P.14-18). This problem is most relevant for the departments of intensive care, where there are infants in critical condition determined by the severity of the underlying disease and increased susceptibility to infection. The importance of the earliest clinical laboratory predict the outcome of respiratory distress syndrome, combined with perinatal Central nervous system, is due to a significant increase in mortality in the group with severe forms of the disease, demanding what their long-term mechanical ventilation. The analysis of literature showed that the issue of predicting the outcome of respiratory distress syndrome and perinatal lesions of the Central nervous system in infants who are on a ventilator, poorly lit, and therefore development in this direction is very important. Currently known methods of predicting the outcome of respiratory distress syndrome and perinatal lesions of the Central nervous system in newborns. A method for predicting the course and outcome of hypoxic-ischemic lesions of the Central nervous system in neonates with RDS according to the patent of Russian Federation №2192779 from 20.11.2002. Using computer analysis assess the quantitative characteristics of spectral indicators power Delta-rhythm and their spatial distribution. If from the first day of the intensive care unit the maximum spectral power observed in occipital and parietal areas, reaching respectively 119-160 µv2and 106-138 µv2and on days 2-3 power characteristics the Delta rhythm reliably prevail in the left hemisphere compared to the right, predicts favorable outcome of the disease. The disadvantages of this method: Refers to additional methods of research, labor-intensive technology, the reliability of the research is connected with multiple recording EEG, called the em to predict the severity of Central nervous system, and only indirectly respiratory distress syndrome. The way to diagnose the severity of respiratory distress syndrome in premature infants with patent RF№2159937 of 27.11.2000. Through biochemical studies of blood plasma proteins determine the protein content and the values of the last above 59% and below 74% of diagnosed respiratory distress syndrome of mild severity, higher 47.1% and below of 58.9% - moderate severity and when the content is below 47% - severe respiratory distress syndrome. Disadvantages of the method: A limited number of patients in preterm infants. Allows you to diagnose the severity of lung lesions, but does not allow a high degree of probability to predict disease outcome in severe respiratory distress syndrome requiring mechanical ventilation. Not possible to judge the state of the immune reactivity of the patient. How to dynamically assess the flow of respiratory distress syndrome by RF patent No. 2153282 from 27.07.2000. Determine the hourly ratio of partial pressure of oxygen in arterial blood to fraction of inhaled oxygen, the distensibility of the lungs, the pulmonary artery pressure. Calculate the rate of change of a ratio of partial pressure of oxygen in arterial blood to fraction of inhaled, the speed of the change of the elasticity of the lungs and the rate of change of pressure in the pulmonary artery on the original mathematical formulas. The effectiveness of the treatment is assessed according to the calculated values when progressing or regressing the course of respiratory distress syndrome (RDS). Disadvantages of the method: Cannot be recommended for use in neonatology. The prototype of the invention is selected the method according to the patent of Russian Federation№2159937 of 27.11.2000. The prototype disadvantages are eliminated in the invention. The objective of the invention is to develop a highly sensitive immunological method of predicting outcome of respiratory distress syndrome in newborns with perinatal CNS damage, being on a ventilator. The problem is solved in that in the blood of newborns enzyme-linked immunosorbent assay (ELISA) to determine levels of endogenous granulocyte colony-stimulating factor (g-CSF) for the admission of patients in the Department and at the level of g-CSF to 1.47 ng/ml and below forecast neblagopriyatnyi outcome of the disease. For statement analysis is used "test-system enzyme immunoassay for determination of granulocyte colony-stimulating factor (g-CSF) human" production LLC Ctoken", Russia, Saint Petersburg attached to the kit instructions. When receiving the newborn trying to enter 1 ml of venous blood in a Vacutainer tube for receiving plasma from the separation gel and EDTA is used as anticoagulant (Becton Dickinson, USA), centrifuged for 3 minutes at 5000 rpm at a temperature of 4°C. 100 μl of the obtained plasma was used for analysis. The optical density is determined on the multifunctional reader "Viktor" (Finland) at a wavelength of 450 nm. Analysis of results is carried out automatically using a computer program "Viktor-Wallak (Finland). An adverse outcome of the disease is predicted at the level of g-CSF to 1.47 ng/ml and below. Prediction of adverse outcome of the disease already at the time of admission to the Department, taking into account the state of the immune status of the organism, allows not only to timely appoint an optimal antibacterial and infusion therapy, but also to use in the early stages of immunomodulating drugs. Our studies revealed that the determination of endogenous g-CSF allows a high degree of probability to predict the outcome of respiratory distress syndrome and perinatal Central nervous system in infants who are on a ventilator. The operability of the invention is evidenced by the following specific examples. Example 1. Child A-s, 1-s night life, history No. 103/4321. The weight status of the child at the time of transfer from the hospital to the intensive care unit was due to respiratory failure in the form of Acro and perioral cyanosis, shortness of breath, the participation of accessory muscles, the indrawing of the sternum, the sharp weakening of auscultation of breath in the lungs on both sides and perinatal Central nervous system in the form of symptoms of oppression - muscular hypotonia, hyporeflexia. Given the parameters of acid-base composition of the blood (hypoxemia, hypercarbia, decompensated acidosis), the child was transferred on a ventilator and transferred to the intensive care unit. In the result of research carried out by the method described above the following results were obtained plasma levels of g-CSF: admission to 1.47 ng/ml on the day 3 of 0.75 ng/ml, in the outcome of the disease - 0.3 ng/ml Detection of plasma levels of g-CSF to 1.47 ng/YPD below to enroll in the resuscitation of the newborn infant with respiratory distress syndrome and perinatal CNS damage, being on a ventilator, testified about the adverse long-term outcome of the disease. On the 5th day the baby died. On pathologic-anatomic opening the newborn had a confirmed diagnosis of multiple atelectasis of the lung, bilateral pneumonia and perinatal ischemia and hemorrhagic lesions of the Central nervous system. Thus, from this example it follows that the detection of plasma levels of g-CSF to 1.47 ng/ml and below at admission to the intensive care unit testified about the adverse prognosis of outcome respirator the th distress syndrome and perinatal CNS lesions in the newborn, located on a ventilator. Example 2. Child R s, the first day of life, history No. 126/5731. Came from a maternity hospital in the intensive care unit on a ventilator due to increasing respiratory failure in the face of severe neurological symptoms at the age of 8 hours. When entering the plasma levels of g-CSF amounted to 1.48 ng/ml against the backdrop of ongoing respiratory, antibiotics and intensive care for 3 days when you study the plasma levels of g-CSF increased to 1.65 ng/ml On the 11th day the child was excubitor and transferred to spontaneous breathing. On the 12th day the patient was transferred to the neonatal care unit for further observation and treatment. At the moment the transfer plasma levels of g-CSF was 1.70 ng/ml. Thus, from this example it follows that the detection of plasma levels of g-CSF to 1.48 ng/ml and above indicates a possible favorable prognosis of the outcome of respiratory distress syndrome in newborns with perinatal CNS damage, being on a ventilator. The proposed method was examined in 38 infants with respiratory distress syndrome and perinatal CNS damage in critical condition in the ICU on a ventilator. 14 children, where plasma levels of g-CSF for admission to the Department do not exceed the concentration of 1.47 ng/ml, had a summer place the capacity outcome of the disease. When pathologic-anatomic study, all patients were found to have multiple lung atelectasis, pneumonia, combined with perinatal CNS damage. In 5 patients, and the section was confirmed diagnosis of neonatal sepsis, a complication for respiratory distress syndrome. 24 children plasma levels of g-CSF for admission amounted to 1.48 ng/ml and above. All of these patients were transferred to the neonatal care unit. Thus, the study upon admission to the intensive care unit plasma levels of g-CSF in neonates with respiratory distress syndrome and perinatal CNS damage, being on a ventilator, lets say that the accuracy of the proposed method is 90.7 percent. Based on the foregoing, the inventive method predict the outcome of respiratory distress syndrome and perinatal CNS lesions compared with existing methods has the following advantages: 1. The method allows to predict the outcome of respiratory distress syndrome and perinatal Central nervous system in infants who are on a ventilator during admission to the intensive care unit, allowing immediate increase of intensive therapy. 2. The method applies to the Express-diagnostics (run time 1 hour). 3. The proposed method oblad is no high accuracy and will find wide application not only in the pediatric intensive care unit, but in other pediatric hospitals. The proposed method for predicting the outcome of respiratory distress syndrome and perinatal Central nervous system in infants who are on a ventilator can be used in neonatology, Pediatrics and intensive care, complementing the known methods of laboratory diagnostics, allowing timely and reasonably assign pathogenetic therapy, increasing survival and reducing the duration of treatment. A method for predicting the outcome respiratornog distress syndrome in newborns with perinatal CNS damage, being on a ventilator, by studying the blood, characterized in that to determine the plasma levels of granulocyte colony-stimulating factor, and when the value of the last of 1.47 ng/ml and below predict adverse outcome of the disease.
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