Method for determining tuberculous spondilitis activity degree |
|
IPC classes for russian patent Method for determining tuberculous spondilitis activity degree (RU 2308723):
 Method for evaluating functional liver condition / 2305844
Method involves determining α-globulin, albumin, β-globulin concentrations and calculating diagnostic criterion value as newborn cattle calves functional liver state coefficient from formula of Kfsl=A/(A+B+C), where A is the α-globulins quantity, B is the albumins quantity and C is the β-globulins quantity. Coefficient Kfsl value being <0.2, functional hepatic insufficiency is diagnosed.
 Method for selecting chronic cardiac insufficiency patients treatable with nebivolol / 2305498
Method involves determining endothelin-1 concentration in blood plasma. Its value being equal to 1.5-5 fmole/ml, treatment with Nebivolol is administered.
Method for differential diagnostics of purulent meningitis / 2305287
In spinal fluid (SF) one should detect the concentration of C-reactive protein (C-RP) and at its value being >2.9 mg/l it is possible to diagnose meningococcal meningitis, at SF C-RP value being ≤2.9 mg/l - nonmeningococcal meningitis. Application of the present method provides the chance to increase accuracy, specificity and information value of differential diagnostics of purulent meningococcal and non-meningococcal meningitis that enables to carry out earlier specific treatment. The method in question, moreover, is very quick and simple in implementation.
Method for evaluating the severity of tuberculosis infection / 2305286
In the experiment upon guinea pigs in blood plasma one should detect the concentration of taurine and glutamic acid and at taurine concentration being below 68 mcM/l and glutamic acid being below 76 mcM/l it is necessary to evaluate the state as severe, at taurine concentration ranged 68-89 mcM/l and that of glutamic acid ranged 125-227 mcM/l - as average severity degree, and at taurine and glutamic acid concentrations being above 89 and 227 mcM/l, correspondingly - as light severity degree. Application of the present method enables to increase information value and significance of the method in question and, also, detect body reactivity according to the state of its different systems.
 Method for predicting diabetic retinopathy / 2304786
Due to biochemical testing lacrimal fluid (LF0 it is necessary to detect the concentration of cholesterol, activity of superoxide dismutase (SOD) and catalase, in blood one should detect the concentration of nitrites and based upon the values obtained calculate Function 1 and Function 2. Function 1 = -3.4+0.29(NO2)+32.5(cholesterol)-0.97(catalase)-17.28(SOD) - corresponds to Y-axis values and Function 2 = -7.14+0.17(NO2)+37.2(cholesterol)+2.1(catalase)-17.28(SOD) - corresponds to X-axis values on the scheme to detect lesion type. If the point obtained is on the field of the 1st scheme one should detect the presence of nonproliferative DR form in a patient at no risk of proliferation; if it is on the field of the 2nd scheme - nonproliferative DR form with the risk of proliferation; if it is on the field of the 3d scheme - proliferative form of retinopathy in the phase of neovascularization. Application of the present method enables to carry out diagnostics in due time, differentiation in doubtful cases directed towards adequate treatment and decreasing the risk for DR progressing.
Method for predicting the development of postgastroresectional peptic ulcers / 2304785
The present innovation deals with predicting the development of postgastroresectional peptic ulcers in patients due to detecting the level of gastrin after stimulation, pepsinogen, prostaglandins PGE2 and PG F2α, correspondingly, in mucosa of gastric stump and in mucosa of gastroenteroanastomosis. At increasing gastrin level up to 12.5 pM/l and higher, that of pepsinogen up to 10.0 mcg/l and higher and decreasing the level of prostaglandin PGE2 up to 570 and lower and at the decrease of prostaglandin PG F2α level up to 652 ng/g and lower one should predict the development of postgastroresectional peptic ulcer. Application of the present innovation enables to increase the accuracy in predicting the development of postgastroresectional peptic ulcers.
Method for predicting the chance for osteomyelitis chronization in children / 2304784
During the moment of exacerbation and on ending the disease in question one should detect the content of alpha-1 proteinase inhibitor and alpha-1 acid glycoprotein in blood due to immunoturbidimetric technique. At decreased content of alpha-1 proteinase inhibitor from 2.3-2.6 g/l up to 1-1.2 g/l, and alpha-1 acid glycoprotein from 2.4-2.6 g/l up to 0.7-0.8 g/l one should predict the absence of osteomyelitis chronization. At decreased content of alpha-1 proteinase inhibitor from 2.9-3.1 g/l up to 1.8-1.6 g/l, and that of alpha-1 acid glycoprotein from 3-3.2 g/l up to 1.2-1.3 g/l one should predict osteomyelitis chronization. Application of the present method enables to predict the disease flow in shorter terms at high significance that provides the chance to prescribe the complex of efficient measures in due time which vary depending upon the disease flow.
Method for predicting the development of gestosis / 2304783
Starting from 22nd wk of pregnancy it is necessary to detect the concentration of neurospecific enolase and glyofibrous acid protein in blood serum. The development of gestosis in pregnant women should be predicted by the value of neurospecific enolase being above 12.4 ng/ml and glyofibrous acid protein being above 4 ng/ml. Moreover, severe gestosis should be predicted at the value of neurospecific enolase being 19 ng/ml and higher and glyofibrous acid protein being 10 ng/ml and higher. Application of the method enables to increase the accuracy of prediction.
Method for evaluating the results of surgical sanitation of inflammation focus in patients with chronic osteomyelitis / 2304781
The present innovation deals with laboratory investigations in case of traumatological pathology. One should simultaneously carry out microscopic investigation of material sample out of inflammation focus and blood from cubital vein to study the growth of microorganisms in the focus and in case of its absence and at registering positive index of digestion in blood with test culture S.aureus 209 P inside neutrophil after incubation, the value of IgM being 0.5-1.3 against the norm, the value of C-reactive protein being up to 12.0 mg/l it is possible to conclude upon satisfactory result of therapy conducted. Application of the present method enables to evaluate the degree of inflammatory process in bony tissue and, also, the state of phagocytic function of neutrophils as the first line of protection against microbial invasion.
Method for predicting the frequency of acute respiratory-viral infections due to evaluating the state of local immunity in children of primary-school age / 2303784
One should detect gamma-glutamyl transferase (GGT) in saliva sampled in the morning to an empty stomach. At GGT values being under 0.85 IU/l or above 3.4 IU/l it is possible to diagnose the decrease of local immunity in children and predict the risk for transferring a healthy child into the group of frequently sick patients. Application of the present method enables to predict the frequency of acute respiratory-viral infections (ARVI) before the procedure of prophylactic vaccinations, provides the chance for carrying out corresponding correction measures. The innovation is simple in application being noninvasive and could be applied in any age group.
Method for predicting the relapse of endometrioid ovarian cyst / 2304782
The present innovation deals with predicting the risk of the relapse of endometrioid ovarian cyst due to immunohistochemical detecting the values of the expression of matrix metalloproteinase-7 and the inhibitor of matrix metalloproteinase-TIMP-4 and according to these values one should predict the relapse of endometrioid ovarian cyst long before the appearance of clinical signs.
 Method for evaluating the state of local immunity of nasal cavity mucosa in rhinological practice / 2297633
In nasal secreta one should detect the content of lysozyme, sIgA, estimate the index of avidity (IA) sIgA followed by calculating the index of immune tension (IIT) by the following formula:
New genes encoding new proteolytic enzymes / 2296160
Invention concerns new polynucleotides, which encode polypeptide having tripeptidylpentidase activity. To produce this polypeptide, host cell is transformed with polynucleotide or polynucleotide-containing vector and then cultured under suitable polynucleotide expression conditions.
 Determination of basidium fungi laccase activity / 2295728
Claimed method includes inoculation of basidium fungus mycelium on nutrient medium. Said nutrient medium contains cellulose, mineral salts and α-naphthol in amount of 0.08-0.12 g/l. Incubation is carried out for 3 days and activity is densitometrically evaluated on the base of linear sizes of colored zones occurred on nutrient medium and coloring intensity thereof.
 Method for predicting genetically deterministic human diseases / 2293992
Method involves determining patient acetylation phenotype and predicting disease from table data built in advance. The table has morbidity coefficients for predicting diseases under prognosis and mortality rates caused by the diseases in countries conditionally uniform with respect to acetylation phenotype, that is, consisting from rapid or only slow acetylators. Prognosed diseases risks and lethal outcome risks caused by those diseases in given age intervals are determined from the values of the coefficients. The risks are predicted in the cases when said coefficient values corresponding to patient phenotype and sex are greater than the same coefficients of opposite phenotype of the same sex with authenticity level equal to or less than 0.05. Clinical course severity is additionally estimated in said age intervals depending on patient phenotype and acetylation degree.
Method for predicting genetically deterministic human diseases / 2293991
Method involves determining patient acetylation phenotype and predicting disease from table data built in advance. The table has morbidity coefficients for predicting diseases under prognosis and mortality rates caused by the diseases in groups of countries where rapid acetylators are predominant in more than 50% of population and in countries where slow acetylators are predominant in more than 50% of population in various age-specific life intervals. Prognosed diseases risks and lethal outcome risks caused by those diseases in given age intervals are determined from the values of the coefficients. The risks are predicted in the cases when said coefficient values corresponding to patient phenotype and sex are greater than the same coefficients of opposite phenotype of the same sex with authenticity level equal to or less than 0.05. Clinical course severity is additionally estimated in said age intervals depending on patient phenotype. Acetylation degree is taken into consideration.
Method for predicting genetically deterministic human diseases / 2293990
Method involves determining patient acetylation phenotype and predicting disease from table data built in advance. The table has correlation coefficients between rapid and slow acetylation phenotype reoccurrence frequency in various countries, morbidity coefficients for predicting diseases under prognosis and mortality rates caused by the diseases in various age-specific human life intervals. The risks are predicted in the cases when patient has rapid acetylation phenotype, only if said coefficient values corresponding to patient phenotype and sex are positive, and in slow acetylation phenotype cases when the coefficients are negative. The risks are predicted in the cases when said correlation coefficient values have authenticity level equal to or less than 0.05. Prognosed diseases risks and lethal outcome risks caused by those diseases in given age intervals are determined from the values of the correlation coefficients in the range from -1 to +1. Clinical course severity is additionally estimated for particular patient in said age intervals depending on acetylation degree settled when determining patient phenotype. Acetylation degree is taken into consideration.
Method for diagnosing clinical course stages of uterine appendages inflammation diseases / 2291438
Method involves determining catalase number and catalase index in menstrual blood sample. The catalase number being from 1.1 to 3.4 units and catalase index being from 0.28 to 0.78 units, acute uterine appendages inflammation diseases are diagnosed. The catalase number being below 0.6 units and catalase index being below 0.14 units, exacerbation of a chronic uterine appendages inflammation disease is diagnosed. The catalase number being from 0.7 to 1.0 units and catalase index being from 0.15 to 0.27 units, chronic uterine appendages inflammation diseases are diagnosed.
 Method for diagnosing metastases in lung root lymph node in pulmonary carcinoma cases / 2284040
Method involves determining lactate dehydrogenase activity and NADP-dependent glutamate dehydrogenase. Lactate dehydrogenase activity being equal to or greater than 12.50 mcU and NADP-dependent glutamate dehydrogenase activity being equal to or less than 0.25 mcU, no metastases in lung root lymph node in pulmonary carcinoma cases are thought to be available. Lactate dehydrogenase activity being less than 12.50 mcU and NADP-dependent glutamate dehydrogenase activity being greater than 0.25 mcU, the metastases are proved to be available.
Method for predicting minimal cerebral dysfunctions in children / 2282858
One should evaluate clinical signs and, additionally, detect the level of neuron-specific enolase (NSE) in blood serum. At NSE content ranged 11.19-18 mcg/l one should conclude upon the diagnosis of minimal cerebral dysfunctions (MCD). Application of the method enables to carry out rational complex of therapeutic measures and establish diagnosis of MCD in short terms at high specificity.
Method of diagnosing small abnormalities in fetus heart development / 2308034
Invention resides in that peripheral venous blood of women at 36-38 weak pregnancy term is assayed to determine amount of CD16+ lymphocytes and antibody/collagen ratio. When value of CD16+ lymphocytes is 14.0% or less and antibody/collagen ratio 0.1 or higher, small abnormalities in fetus heart development are diagnosed. Method is simple to perform and allows diagnosing independent of dimensions, position, and degree of mobility of fetus.
|
FIELD: medicine. SUBSTANCE: method involves studying intracellular lysosomal cation proteins availability in blood granulocytes by means of cytochemical lysosomal cation test and myeloperoxidase and lactoferrin serum proteins concentration in parallel to it. Active tuberculous spondilitis diagnosis is set when having lysosomal cathione test value ≥ 1.6 mean cytochemical coefficient, myeloperoxidase > 200 ng/ml and lactoferrine > 1300 ng/ml. EFFECT: high accuracy of diagnosis.
The invention relates to medicine, namely to Phthisiology, and can be used to determine the activity of tuberculous spondylitis. Tuberculous spondylitis is a chronic long flowing wavy disease, treatment is not always leads not only to cure, but even remission. The determination of the activity of tuberculosis in the spine is necessary to clarify the stage of the disease, treatment, determine the duration of TB treatment and monitoring treatment effectiveness. Known clinical and x-ray methods for determining the activity of tuberculous spondylitis (1). Known laboratory methods for determining the activity of tuberculous spondylitis by identifying changes in the formula of blood, immunological and biochemical indicators(2, 3, 4, 5, 6). In the hemogram frequency of pathological deviations of various parameters ranging from 7.5% (lymphopenia) to 77.8% (accelerated ESR). In biochemical indicators of pathological deviation from 17.6 per cent (level VNSM) to 89% (the activity of the enzyme ADA). In immunological parameters identified frequency determine PTAT 75%, increase in IgA in 84,6%, IgM in 61,5%, IgE in 53.9% of cases. Closest to the present invention is a method for determining the activity of tuberculous spondylitis way the laboratory, including alpha-2-globulins, gamma-globulins, A/G ratio, CRP, ceruloplasmin, haptoglobin, excretion of hydroxyproline in the urine (7). In this way some of the indicators, frequently with active tuberculous spondylitis cannot be used to determine the activity of the process, as and when calmed down spondylitis frequency of occurrence of great. In particular, ceruloplasmin found in 71,4% active spondylitis and in 72.2% of its consequences, the excretion of hydroxyproline in urine in 95,2% with the active process and 42,1% upon impact. To detect the activity of tuberculous spondylitis requires the definition of a significant number of parameters. The disadvantages of the known methods for determining the activity of tuberculous spondylitis are: first, not enough high incidence of pathological indicators with active tuberculous spondylitis; secondly, the frequent occurrence of pathological indicators and with the active and the inactive process; thirdly, the need for a variety of studies to determine the activity of tuberculosis in the spine. It is known that neutrophil granulocytes (NG) is one of the key cells in innate immunity reactions, which play an important role in the development of the inflammatory process (8). NG pic is by phagocytose microbes due to the presence in their granules microbially substances (myeloperoxidase, lactoferrin, defensins and other proteins and peptides). The myeloperoxidase (MPO) and lactoferrin (LF) are sensitive markers, allowing to carry out differential diagnosis of some viral and bacterial diseases. In particular, in viral infections LF serum is reduced, and purulent meningitis increases. IGOS increases in viral peczuh, and purulent meningitis, but when purulent meningitis is much higher. How to use the functional parameters NG peripheral blood to determine the activity of tuberculous spondylitis are not described in literature. The objective of the proposed method is to improve the accuracy of diagnosis of active tuberculous spondylitis and reducing the number of the studied parameters. The problem is solved by a parallel study of the content of intracellular and serum individual lysosomal cationic proteins neutrophilic granulocytes blood and when the value of lysosomal cationic test ≥1,6 CCS, myeloperoxidase ≥200 ng/ ml lactoferrin ≥1300 ng/ml diagnose active tuberculosis spondylitis. The method is as follows. The intracellular content of cationic proteins (defensins and related peptides) in the lysosomes NG blood was evaluated using a t chimicheskogo lysosomal cationic test (LKT) according to the method V.E. Egorevskoe (9). Fixation and staining of smears of blood was performed using 0.1% solution of the dye fast green in 0.1 M Tris-HCl buffer, 50% methanol (pH 8.1-8.2) for 20 minutes, after which the drugs were washed out and they finished painting areas 0.25% aqueous solution of Azura And to identify cell nuclei. The total content of cationic proteins in NG was determined semi-quantitatively by the average cytochemical coefficient based on the following formula:
The CCS medium cytochemical factor. Letters (a-g) indicate the number of similar painted solid green cells, and the numbers (3-0) - the degree of staining intensity in the cytoplasm NG, n is the number NG (at least 100). Determination of serum proteins myeloperoxidase and lactoferrin produced by the method of enzyme-linked immunosorbent assay (10): wells ELISA plate (Falcon 3912 Microtest III Flexible Assay Plate) made of 100 μl of a solution of anti-MPO (anti-LF) at a concentration of 4 μg/ml in 0.1 M carbonate-bicarbonate buffer, pH 9.5) and incubated overnight at room temperature. Then the tablets is not less than 4 times washed with a buffer for washing (0.01 M Na-phosphate buffer pH of 7.2, containing 0.15 M NaCl and Tween-20). In the washed wells were made in 100 μl of buffer for washing with 1% bovine serum albumin (BSA), and incubated 1 hour at room temperature. Then the buffer UDA is Yali and wells made the analyzed samples (serum) in a volume of 100 μl, diluted to the working concentration of the buffer for washing with 1% BSA 10 and 100 times. The same buffer was making serial dilutions of a standard solution IGOS (LF) to obtain the following concentrations: 80, 40, 20, 10, 5, 2,5, 1,25 ng/ml. the plates were incubated overnight at 4°C. After incubation, the tablets were washed with buffer for washing contributed 100 ál of HRP-conjugate, anti-MNO-HRP at a dilution of 1:2000 (anti-LF-HRP at a dilution of 1:20000) (diluted with buffer containing 1% BSA) and incubated over night at 4°C. Then the tablets carefully washed and made of 100 µl of substrate solution for HRP (0.2 mg/ml o-phenylenediamine in 20 mm citrate buffer pH 5.0 containing 0.5 μl/ml 30% H2O2), the reaction was stopped by adding 50 µl of 2.5 M H2SO4measured extensio at a wavelength of 492 nm (Multiscan). On the basis of standard dilutions IGOS (LF) constructed a calibration curve and determined the content of IGOS (LF) in the samples. The result was correlated with the threshold, components for LKT - 1,6 CCS, IGOS - 200 ng/ml and LF - 1300 ng/ml When the value LKT ≥1,6 CCS, IGOS>200 ng/ml and LF>1300 ng/ml was determined that tuberculous spondylitis is in an active stage. When the value LKT<1,6 CCS, IGO<200 ng/ml and LF<1300 ng/ml was determined that tuberculous spondylitis is in the inactive stage. Approbation of saleemullah are the results of a survey of two groups of patients with tuberculous spondylitis, a total of 39 people. The 1st group consisted of 27 patients with tuberculous spondylitis in stage heat, 2-n - 12 patients with tuberculous spondylitis in a stage of remission. It turned out that in group 1 LKT was ≥1,6 CCS (1,6-1,99 CCS) in 26 patients, one patient LKT was 1,53 CCS. Therefore, the sensitivity of the proposed method, by definition, LKT is equal to 96.3%. IGOS was >200 ng/ml (223-951 ng/ml) in 25 patients. The remaining 2 patients IGOS was <200 ng/ml and expressed 135 and 162 ng/ml Therefore, the sensitivity of the proposed method for the determination of IGOS is 92,6%. LF >1300 ng/ml (1361-6208 ng/ml) was detected in 27 patients. The sensitivity of the proposed method for the determination of the LF is 100,0%. Thus, the complex LKT+IGO+LF sensitivity of the method is equal to 100%. In group 2 LKT all 12 patients was <1,6 CCS (1,31-1,56 CCS), therefore, the specificity of the test LKT is 100%. IGOS in 11 patients was <200 ng/ml (74-162 ng/ml)in 1 patient IGOS was above the threshold level and amounted to 207 ng/ml Therefore, the specificity of the test MVE 91.7%. LF <1300 ng/ml (326 - 1292) was detected in 10 patients, LF >1300 ng/ml in 2-1531 and 1795 ng/ml Specificity of the proposed method on LF is 83,3%. Thus, the specificity of the proposed complex LKT+IGO+LF is 83,3%. In General, the TLD is the group of patients with active tuberculosis in the spine correctly defined in 37 cases out of 39. Therefore, the diagnostic efficacy of the test on three indicators LKT+IGO+LF equal 94,9%. Examples of implementation of the proposed method. 1. Patient P., aged 30 ill tuberculous spondylitis 3 years, within the last 6 months had received anti-TB treatment with improvement. Radiographically: areas of destruction in the bodies of Th 7-10 vertebrae. In the clinical analysis of blood no changes. LKT - 1,6 CCS, IGOS - 943 ng/ml, LF - 6208 ng/ml Process is recognized as active. During the operation was found areas of destruction in the bodies of Th 7-10 with granulations and pus. The patient in the postoperative period was conducted on TB treatment within 12 months. 2. Patient B., 53 years, suffering from tuberculosis of the spine 35 years, said the deterioration in the last 3 years in the appearance of pain and neurological disorders. Radiographically: areas of destruction in the bodies of Th 7-12. In the clinical analysis of blood without pathology. LKT - 1,56 CCS, IGOS -152 ng/ml, LF - 492 ng/ml. Process in the spine recognized calmed down. During the operation detected dry calcified caseous mass without signs of active TB. The patient in the postoperative period was conducted on TB treatment within 2 months. Thus, the proposed test allows to increase the accuracy of determining the activity of tuberculous spondylitis, to reduce the number of studies is required to determine the duration of TB treatment. Literature 1. Kornev p. g Surgery of osteoarticular tuberculosis. - L., 1971. - V.3.- P.46-50. 2. Extrapulmonary TB /edited Avecilla. - SPb, 2000. 49 - 56, 123-144, 192-195 (in Russian). 3. The use of standardized multilevel algorithm immunodiagnostic tuberculosis in different locations in modern epidemiology: a Manual for physicians / Comp. Avecilla, Riendeau, Nemcova etc. - Petersburg, 2000. - 32 S. 4. Titarenko O.T., Dyakov M.E. Ter-Minassian, Perov TL and other Biochemical criteria for differential diagnosis of tuberculosis and osteomyelitis of the spine //Tuberculosis in the North-West region of Russia: modern problems. - SPb, 2002. - S-116. 5. Gusev V., Ivanov V.M., Potapenko H. and other Immune status of patients with active tuberculous spondylitis //Probl. the tubes. and lung diseases. - 2003. No. 6. - C-28. 6. Bone and joint TB /edited Unevasive and Aesalus. M., 2003. 49 - 54. 7. Titarenko O.T., Perov TL, Gusev V., Momot DS., Tikhodeev S.A. Biochemical characteristics of the process activity in tuberculous spondylitis. Deponer in NIIMI. No. 17682, 1989. 8. Ivanov V.M., Gusev V., Shenderova RI and other Clinical and laboratory features of tuberculosis and osteomyelitis of the spine// Problems of tuberculosis and lung diseases. - 2003. No. 10, p.34-37. 9. Kokryakov NR. Biology of antibiotics animal - SPb, 1999. - 70 S. 10. Pisarevsky VE Lysosomal cationic test: Method, writing. - L., 1987. - 13 S. 11. Batiashvili NM, Aleshin G.M., Sorokina M.N., Ivanov V.V. Myeloperoxidase and lactoferrin in blood serum and cerebrospinal fluid of children with meningitis. //Honey. immunology. 2002. V.4. No. 4-5, S-572. The method for determining the activity of tuberculous spondylitis, by laboratory studies, characterized in that parallel to learn the content of intracellular lysosomal cationic proteins of neutrophilic granulocytes in the blood using a cytochemical lysosomal cationic test and the content of the whey proteins myeloperoxidase and lactoferrin, when the value of lysosomal cationic test ≥1,6 CCS, myeloperoxidase >200 ng/ ml lactoferrin >1300 ng/ml diagnose active tuberculosis spondylitis.
|