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IPC classes for russian patent Modified chitosan (RU 2269542):
                   
Method for preparing chitosan polyethylene glycol ester / 2266915
Invention relates to methods for preparing chitosan esters. Invention describes a method for preparing chitosan polyethylene glycol ester that involves dissolving chitosan in acetic acid followed by alkalization. Then the reaction mixture is subjected for effect of ethylene oxide under pressure 1-3 atm and temperature 60-100°C, and the concentration of reaction mass is corrected by addition of distilled water up to the density value of solution 1.030-1.032 g/cm3. Then the reaction mass is purified by electrodialysis at the rate value of solution in treatment chambers 3.0 cm/s, not less, temperature 20-45°C, the current density value 0.25-0.75 A/dm2 and the constant volume of the reaction mass. Method provides enhancing the effectiveness of purification by electrodialysis due to reducing energy consumptions. Chitosan esters can be used in medicine, cosmetics, food and chemical industry.
 Method for preparing water-soluble derivatives of chitosan / 2263681
Invention describes a method for preparing water-soluble derivatives of chitosan. Method involves treatment of chitosan with acid medium up to its swelling wherein vapor medium water-acid is used as acid medium. Treatment of chitosan is carried out with vapor of monobasic acid aqueous solution taken among the group including hydrochloric acid, formic acid and acetic acid. Method allows simplifying technology in preparing water-soluble derivatives of chitosan.
Method for chitosane production / 2250909
Claimed method includes subsequent chitosane-containing raw material with non-polar liquefied gas, water, alkali, water, acid, water, alkali, and water to produce target product in form of solid residue, wherein in at least first extraction step pressure in reaction mixture is periodically released to provide extractant boiling, and than increased up to starting value.
Method for producing of chitosan from chitin of cancerous / 2246880
Method involves providing deacetylation of raw material with the use of preliminarily cooled alkaline solution; washing and drying. Deacetylation process is performed in three stages, first stage being performed for 7 days and subsequent two stages being performed for 2 hours each, combined with thermal processing at temperature of 55-590C. Washing process is provided after each deacetylation stage.
 Macroporous chitosan granules and method of production thereof, a method of culturing cells / 2234514
The invention relates to macroporous chitosan granules having a relatively large and uniform pore size of 30-150 μm inside and outside, which are distributed from the surface to the area of the nucleus, and the way they are received, which includes the following stages: adding dropwise chitosan solution, the aqueous chitosan solution, or a mixture thereof in the low-temperature organic solvent or liquid nitrogen; regulation of pore size using the method of phase separation due to temperature differences
The method of obtaining innovating derivatives of chitosan oligosaccharide / 2232775
The invention relates to a method for producing derivatives of chitosan oligosaccharide and can be used in medical and food industries, as components of various compositions
 Method cross-linkage carboxylating polysaccharides / 2230073
The invention relates to a method of cross stitching carboxylating polysaccharides and can be used in medical and pharmaceutical fields, and in cosmetology
A method of obtaining modified chitosan emulsions and products on its basis / 2223279
The invention relates to chemical technology, particularly to a method for producing a gel or suspension of chitosan, and can be used in food, cosmetic, pharmaceutical and other industries
A method of producing chitosan / 2221811
The invention relates to a method of producing chitosan, which can be used as a sorbent, emulsifier, foaming agent
 The method of obtaining food biosorbent / 2219997
The invention relates to the microbiological industry, and specifically to methods for biosorbent by microbiological synthesis, which can be used as activator beer fermentation
Method for revaccination in adults against diphtheria and tetanus in far north / 2268746
The present innovation deals with technique of revaccination in adults against diphtheria and tetanus under conditions of Far North. For this purpose one should form a group of people vaccinated more than 10 years ago to vaccinate them in February. The method leads to increased efficiency of vaccination due to decreased duration of vaccination period.
Method for preventing placental deficiency under conditions of urogenital infecting with chlamydiosis / 2268740
One should conduct antibacterial therapy 3 mo before planned pregnancy consisting of preparations of tetracyclinic group or macrolides in combination with nystatin and metronidasol to continue it since the first day of menstrual cycle and, also, it is necessary to perform correction of vaginal biocenosis, and about 3-4 wk after therapy carried out for 2 next mo before planned pregnancy starting, also, since the first day of menstrual cycle - metabolic therapy; At the onset of pregnancy one should conduct courses for preventing placental deficiency dealing with introduction of preparations that improve uterine-placental circulation, and preparations that improve rheological properties of blood and vitamin-metabolic therapy or the same preparations, and additionally - introduction of macrolides ad viferon rectally in case of activation of chlamydial infection. The present innovation enables to sanitize uterine cavity, improve uterine-placental circulation, restoration of placental tissue structure that, in its turn favors the birth of healthy generation.
Medicinal formulation possessing bacteriostatic effect and method for its preparing / 2268051
Medicinal formulation possessing the bacteristatic effect consists of a core comprising the following components, wt.-%: clarithromycin, 40.0-80.0; polyvinylpyrrolidone, 3.0-10.0; sodium lauryl sulfate, 2.0-5.0; sodium croscarmelose, 4.0-10.0; aerosil, 0.5-2.0; magnesium stearate, 0.5-2.0, and microcrystalline cellulose, 10.0-31.0. Also, the medicinal formulation consists of envelope comprising the following components, wt.-%: hydroxypropylmethylcellulose, 30.0-70.0; polyethylene glycol, 12.0-22.0; titanium dioxide, 11.0-20.0, hydroxypropylcellulose, 2.0-10.0, dye yellow quinoline, 1.0-4.0, and vanillin, 1.0-4.0. Also, invention describes a method for preparing the medicinal formulation by wet granulation followed by tableting and applying the envelope from an aqueous suspension. Prepared tablets show the necessary mechanical strength, insignificant scattering index by mass (± 3.5%) and dissolving 88-91% for 30 min.
Method for treating genital fistulas / 2268044
One should introduce 3.5%-chitosan ascorbate gel into fistulous channel that contains metronidasol at the dosage of 2 mg/ml, at the volume up to 20 ml once/2 d till complete fistula's closing. The present innovation enables to activate reparative processes and fistulous epithelization that favors for closing fistulous channel in earlier terms.
Method for post-operational treatment of donor's wounds / 2265454
One should apply a polycompositional film onto donor's wounds after autodermoplasty performed. This film contains the following components in weight proportions: chitosan 78.3-89.4; polyvinyl alcohol 9.8-19.8; antibiotic of aminoglycoside group 0.5-2.0; anesthetic 0.1-0.2. It is perforated at tension coefficient being 1:4. The innovation enables to decrease wound's traumatization, improves prophylaxis of suppuration and increases cosmetic effect even at a single application of the film suggested.
 Application of pleuromutilin derivatives for transdermal treatment of bacterial disease / 2264812
The present innovation deals with application of pleuromutilin derivatives, that is valnemulin and thiamulin, for transdermal treatment of bacterial diseases, in particular those induced by Dichelobacter nodosus, Fusobacterium necrophorum, Bacteriodes nodosus and Bacteriodes melamnogenicus, for manufacturing medicinal preparation or as an active ingredient of medicinal preparation of the same indication and corresponding method for transdermal treatment of diseases, for example foot rot. It has been detected the capacity of antibiotics to penetrate skin and enter either plasma or blood at concentrations being efficient against systemic bacterial infections, so, medicinal preparation could be designed in the form of ointment, cream, solution, shampoo, powder and spray.
Secretory immunoglobulin a preparation possessing antiviral or antibacterial effect / 2264229
Invention proposes preparation that comprises the immunoelectrophoretically pure secretory immunoglobulin A isolated from whey milk and/or colostrum of immunized ungulate animals and pharmaceutically acceptable vehicles. The base preparation (substance) comprises 6-12% of secretory immunoglobulin A at pH 4-8, an anti-complementary activity at least 10 mg of protein, not activating 2 CH50, protects in >70% against corresponding infections (in infection macroorganism in doses ≥10 ID50), shows areactogenic property in intravenous administration, can comprise stabilizing additives in the total concentration 4%, not above. The preparation possesses high purity, low anti-complementary activity, stable in storage, useful for oral, parenteral and topical using and possesses therapeutic activity with respect to microorganisms and viruses against which humans and animals immunization have been carried out. Invention can be used in treatment and prophylaxis of immunodeficiency states, bacterial and viral infections in humans and animals.
 Heterocyclic compounds comprising 2-aminopyridine-3-sulfomic fragment, methods for their preparing (variants), focused library and pharmaceutical composition / 2263667
Invention relates to novel heterocyclic compounds comprising 2-aminopyridin-3-sulfonic fragment of the general formula (1)
 Heterocyclylsulfinic acids and their derivatives, focused library and pharmaceutical composition / 2263666
Invention relates to new heterocyclylsulfonyl alkylcarboxylic acids and their derivatives of the general formula (1):
 Method for preparing 4''-substituted derivatives of 9-deoxo-9a-aza-9a-homoerythromycin a / 2263117
Derivative of 9-deoxo-9a-aza-9a-homoerythromycin A of the formula (3) wherein R4 represents hydroxyl protecting group is prepared by protection of 2'-hydroxy-group of compound of the formula (5) to form compound of the formula (4)
 Composition for treatment or prophylaxis of infection caused by microorganism neisseria / 2244749
Invention relates to a composition designated for treatment and prophylaxis of infections caused by Neisseria microorganism that comprises the following components: (a) protein with amino acid sequence similar by 65% and above with the natural Neisseria protein of a single species (the first group of amino acid sequences is given in the text) and/or its fragment consisting of 10 and more amino acids and eliciting antigen properties; (b) the second protein with amino acid sequence similar by 65% and above with the natural Neisseria protein of another species (the second group of amino acid sequences with even numbers is given in the text), and/or its fragment consisting of 10 or more amino acids and eliciting antigen properties; in particular, the second protein represents NspA. The composition comprises additionally adjuvant. The composition is used both a medicinal agent and for manufacturing the medicinal agent. Applying the invention provides enhancing the effectiveness of prophylaxis or treatment due to the universal effect of the composition (vaccine). Invention can be used in medicine for treatment of infections.
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FIELD: organic chemistry of natural compounds, chemical technology, medicine. SUBSTANCE: invention relates to the group of chitosan-containing compounds. Invention relates to synthesis of modified chitosan of the following structure: EFFECT: valuable medicinal properties of modified chitosan. 1 tbl, 1 dwg, 3 ex
The invention relates to the field of chemical technology, namely the group of compounds containing chitosan. The resulting modified chitosan can be used in medicine, cosmetics, agriculture, veterinary, food and light industry. Chitosan is a physiologically active compound of natural origin, widely used in cosmetics and medicines. It is produced by hydrolysis of chitin, the main component of the outer skeleton of arthropods:
n=150-1400 Chitin (from the French "chitine" and the Greek "chitone", which means "skin", "shell") is widely distributed in nature, it is part of the cell walls of fungi and bacteria, where does the support and a protective function. According to the chemical structure of chitosan is a high molecular weight polymers (MM˜30000-260000 USD) from the group of polysaccharides built from the remnants of glucosamine (acetylglucosamine), interconnected β(1→4) glycosidic bonds. In its structure, physicochemical properties and biological role of chitosan is similar to the cellulose of plants. In recent years in various fields of medicine and cosmetology has emerged a heightened interest in the use of chitosan as a mild remedy, obladayushzuyu antiseptic effect. Especially valuable is that chitosan, being a product of vital activity of living organisms are harmless to humans. Known to be an effective antibacterial antibiotic streptomycin, which molecule is a tricyclic carbohydrate containing one of the two cycles guanidine groups [encyclopedia of medicine. 8th ed., edited Ufraw, M., "radar", 2001, str]. The structural formula of streptomycin has the following form:
Streptomycin by their chemical nature, as chitosan refers to the aminoglycosides, is an effective broad-spectrum antibiotic, active against Mycobacterium tuberculosis, the causative agents of plague, tularemia, brucellosis, dysentery, strepto-, fees and meningococci, produces radiant fungus (genus Streptomyces). However, streptomycin is toxic enough and with long-term use affects the auditory nerve. Closest to the proposed solution is the drug carboxymethylchitosan, obtained by the reaction of chitosan with monochloracetic acid (U.S. Pat. Of the Russian Federation No. 2185387, class 08 In 37/08, 2000). The disadvantages of this drug include lack of bactericidal activity and complex method of receipt. The technical purpose of this invention is to increase the bactericidal activity and the drug. To solve the technical problem of the synthesized modified chitosan of the following structure:
where n=150-1400 Since the polymer forms of medications are generally preferred due to the strengthening effect due to the presence of many active groups located at the molecular distances, lower toxicity, and the effect of Deposit (longer therapeutic effect of the drug, it was considered useful to carry out guanidine amine groups of chitosan, turning them from inactive amine in high-performance guanidine. The modified chitosan was obtained as follows. Due to the low thermal stability of chitosan as a soft guanidinium tool was selected carbodiimide (NH=C=NH) - tautomer Monomeric cyanamide (NH2CN). As a source of chitosan materials have served two commercial sample of chitosan succinate companies CJSC "Bioprogress" and "Hemmed", freeze-dried method. Their molecular masses are according to ultrafiltration (osmosis on the membranes) 30000 26000 and; the degree of polymerization, respectively, n=150 and n=1400. The reaction was carried out with swollen chitosan succinate in a solution of acetonitrile containing the estimated quantity of 50% aqueous carbodiimide:
where n=150-1400 To complete the process the reaction mixture after 24 hours of exposure under normal conditions was heated for 10 hours at 40°C. the Modified chitosan differs from the original by more friable structure. IR-spectroscopic study of the obtained polymer (see drawing) shows the appearance of the structure of the modified chitosan (curve 1) a significant number guanidino groups: intensive bands The emergence of guanidino groups in the chitosan is logged chemical analysis: colorimetry with eosin. By the method of serial dilution of the antibacterial activity grandravine chitosan. Bacteriostatic activity on the strain of Pseudomonas aeruginosa was 0,075%, bactericidal 0,15%. Due to the proximity grandravine chitosan to streptomycin drug was also tested for anti-TB activity. It also noted increased efficiency, due to the polymeric nature of the drug (compared with streptomycin) and compared to the unmodified chitosan. Provide examples illustrate the practical implementation of the synthesis grandravine chitosan. Example 1 A portion of 25 g (0.1 mole) of chitosan succinate (n=150) was ground in a coffee mill in a fine yellow powder and was mixed in a flask with 100 ml of acetonitrile, containing 4.5 g (0.1 mole) carbodiimide. After swelling of chitosan in a solution of acetonitrile during the day the reaction mixture to complete the reaction was heated with occasional stirring at a temperature of 40°C. the Modified chitosan was separated by filtration, then washed with 50 ml distillirovanna the water on the filter and dried in air. Received guanidine of modificat chitosan is a volumetric light powder light yellow color, it is difficult soluble in water and slightly better in 2% acetic acid solution. Example 2 A portion of 25 g of commercial chitosan succinate (n=150) was purified by presidenial. To this end it was dissolved in 1.5 liters of 2% aqueous solution of acetic acid. Complete dissolution of the sample chitosan requires at periodic stirring ˜48 hours. The obtained homogeneous solution of chitosan acetate decantation from a small residue, which may include nephrolitiasis chitin and protein impurities. A clear solution was added with vigorous stirring to 2 liters of a 15% aqueous solution of ammonia. The reaction mixture was left for a day and then gently decantation of the upper clear solution. To the precipitate, having a form of white wool was added 1.5 l of distilled water, thoroughly mixed, and again left for the day. The operation of the washing and decanting was repeated three times until the complete disappearance of the smell of ammonia. After that sediment base of chitosan was obezvozhivani on the centrifuge and neutralized by adding dropwise 5 g glacial acetic acid, thorough mixing in a mortar and drying in air to constant weight. Exit 20, The obtained chitosan acetate used in the reaction with the carb what diimides by the method of example 1. The degree of guanidine purified chitosan higher (75%)than neprevzojdennogo (60%), according to the colorimetric determination with eosin. Sample of 0.5 g of the modified and the original chitosan was dissolved in 100 ml of 2% aqueous solution of acetic acid and measured the results of the specific viscosity of the obtained solutions. Close values obtained viscosities ηRef=0.4 ηmod=0,3 indicates the absence of destruction of the chain of chitosan at grandravine. Example 3 Portion 5 g of the commercial chitosan succinate (n=1400) modified by treatment of 1 g of the carbodiimide in 20 ml of acetonitrile by the method of example 1. The modified chitosan was washed on the filter and dried to constant weight. Weighed to 0.1 g of the modified and the original chitosan was dissolved in 100 ml of 2% aqueous solution of acetic acid and measured the results of the specific viscosity of the obtained solutions. Close values obtained viscosities ηRef=1,0 ηmod=0,9 indicates the absence of destruction of the chain of chitosan at grandravine.
The modified chitosan of the following structure:
where n=150-1400.
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