Method for treating genital fistulas
FIELD: medicine, gynecology, surgery.
SUBSTANCE: one should introduce 3.5%-chitosan ascorbate gel into fistulous channel that contains metronidasol at the dosage of 2 mg/ml, at the volume up to 20 ml once/2 d till complete fistula's closing. The present innovation enables to activate reparative processes and fistulous epithelization that favors for closing fistulous channel in earlier terms.
EFFECT: higher efficiency of therapy conducted.
The invention relates to medicine, namely to obstetrics and gynecology, and can be applied in proctology, urology, surgery and dermatology.
Among the most serious diseases of women, which not only leads to multi-organ disorders, long-term permanent disability, but also causing severe physical and mental suffering, menstrual and reproductive functions are genital fistula .
Known widely used surgical treatment of genital fistula, which consists in stitching the fistula is not earlier than 4 months from the moment of its occurrence, and after the edges of the fistula is not already granularit, and healed . The main stages of the closure of fistula - excision of the scar tissue surrounding the edges of the openings of the fistula, and the splitting of the edges of the fistula, the suturing of the defect. The specific localization of the fistula has its own methods of operational benefits, but they all require time, very often leading the woman to disability, which reduces the quality and usefulness of her life . The severity of clinical manifestations and high recurrence rate raises issues of surgical treatment and prevention of genital fistula in a number of priority problems .
Conservative therapy consists in carrying out local and General events. Local treatment is douching is legalise 1% chlorhexidine or 1-3% solution of potassium permanganate, drainage him, the exposure threshold of the fistula by means of mirrors and summing it with the swab or introducing balsamic liniment on Avissawella. Tampon left in the day, through the day the procedure is repeated. After cleansing the wound liniment replaced with jelly or ointment solkoserila and use them to complete epithelialization of the wound .
However, currently applied topically balsamic liniment on Avissawella along with the obvious positive impact on the disease usually causes a number of side effects: allergic reactions like itching, swelling and hyperemia of the mucous membrane of the vagina that many women are unable to use it due to severe hyperemia . The need for long time daily use of a treatment room for conducting the remediation and change dressings to complete epithelialization of the wound causes the woman to abandon their traditional way of life and activity.
The objective of the invention is to improve the effectiveness of the closure of genital fistula, reducing treatment time and lower rate of recurrence of the fistula.
The task of reach due to the fact that after treatment of the walls of the vagina with an antiseptic solution in fistulous entrance impose a 3.5% gel of chitosan ascorbate, containing metronidazole at a dose of 2 mg in 1 ml in volume to 20 ml of 1 every 2 days to complete the closing of the fistula. Treatment leads to the closure of the fistulous course early results fistulografii and wound healing.
The technical result of the high therapeutic effect is significant vascularization of the wound surface of the fistula and early epithelization of the defect [4-6], and selective antibacterial effect .
The method is as follows: in the treatment room the woman laid on the gynecological chair, conduct reorganization of the vulva and vaginal walls with an antiseptic solution, enter the vagina mirror Cuzco, reveal the eve of the fistula by means of mirrors, is injected into the fistula olive cannula with 3.5% gel of chitosan ascorbate, containing metronidazole at a dose of 2 mg in 1 ml in volume to 20 ml of 1 times in 2 days.
The proposed method treated five patients aged 26 to 40 years. All women on the basis of typical clinical signs and results of fistulography identified genital fistulas (2 women brunetoochka-ignorantly fistula, 2 women actually genital fistula, 1 women cervico-vaginal fistula). The disease duration ranged from 2 weeks to 2 years. Patients with brunetoochka-ignorantly fistula complained of gnetaceae of fistulous openings in the anterior abdominal wall and the anal opening, when in fact genitals the x fistula - pain in the perineum and purulent discharge from the vagina, in cervico-vaginal fistula - rich homogeneous discharge from the genital tract with an unpleasant odor, menstrual cycle and miscarriage. The results showed that using the proposed method in the treatment of genital fistula treatment time was 10 to 20 days.
Clinical example 1: patient P., 38 years old, was admitted 21.11.03 in the gynecological Department of the emergency hospital, Krasnoyarsk with a diagnosis of exacerbation of chronic bilateral adnexitis with the formation of tubo-ovarian tumors on the left and pyosalpinx right on the background of intrauterine devices. The operation - Nizhneserginsky laparotomy, adnexectomy left, tubectomy right, dissection of abdominal adhesions, sanitation and drainage of the abdominal cavity. Operations in the abdomen found 50 ml of purulent content, massive adhesions, chronic infiltrative omentitis, when the division of adhesions deseration rectosigmoidal part of the colon for 8 cm, sewn in individual nylon sutures. On the 5th day of drainage is to the left stood out abundant intestinal contents. For 12 hours in the area of the drain holes installed aspirazione-flow system Cansino. At 24 days, the system removed - formed fistula (drainage hole departs mind the counter intestinal contents). The local inspection found fistulous opening in the skin the size of 5x3 cm, expressed maceration and infiltration of the tissue around the fistula, the depth of the fistula on the probe 14 cm, the daily loss of up to 500 ml of intestinal contents. After treatment of the vaginal walls with a solution of furacin in fistulous course introduced Oliva cannula with 3.5% gel of chitosan ascorbate, containing metronidazole at a dose of 2 mg in 1 ml in a volume of 20 ml. of the introduction of the gel was carried out by 1 every 2 days for 12 days. 36 day results fistulografii fistulous course in the gut is not detected. On the skin in the area of the drainage holes on the left - soft scar healing. The patient received as a General antibacterial treatment, detoxification, immunokorrigiruyuschuyu therapy. In a local survey on the background of the application of the 3.5% gel of chitosan ascorbate with metronidazole disappeared maceration of tissues, fistulous course no. Thus, six application of chitosan gel for 12 days resulted in complete elimination of the fistulous course. Patient no complaints, was discharged from the hospital in satisfactory condition. After 9 months on check - fistulous course no, there are no complaints, the woman leads a normal life.
Clinical example 2: puerperal S., 34, gave birth to 23.03.03 in the p/No. 5, Krasnoyarsk live full-term baby boy. When viewed as an obstetrician-gynecologist detection of the n cervico-vaginal fistula, according to women, fistula functions 2 years after a previous birth (cervixes). The treatment gel chitosan ascorbate containing metronidazole. After sanation of a vagina 1% solution dioksidina in the course of the fistula entered Oliva cannula with 3.5% gel in a volume of 10 ml of 1 every 2 days until complete closure of the fistula. After discharge from the maternity hospital treatment continued in the antenatal clinic No. 3. On the 20th day from the start of treatment fistulous course according to fistulografii not detected, when viewed in the mirrors - the cervix and the vaginal mucosa pale pink, mucous allocation, odorless, bimanual vaginal examination the uterus is normal in size and consistency, the uterus is not palpable. At the control examination after 6 months - fistulous course not, menstrual cycle regular, pain and pathological secretions there. A woman on maternity leave to care for a child, the quality of life has improved significantly.
Thus, the proposed method for the treatment of genital fistula using the introduction in the course of the fistulous olive tip with gel chitosan ascorbate and metronidazole allows you to:
1. - To increase the effectiveness of the closure of genital fistula on the basis of their vascularization of the wound surface and the removal of pathogenic microflora, reduce or eliminate, thus, vaginal discharge, the value is positive to reduce or remove the pain for 2-3 s procedure.
2. To reduce the treatment time to 20 days
3. - Eliminate the recurrence of the fistulous opening stroke.
4. To return women to their normal lives, to give them the opportunity to have repeated positive flowing pregnancy and delivery.
1. Krasnopolsky V., Buyanova, S., Genital fistula, M.: "Medpress", 2001, s.
2. Pervanidou A.S., Operative gynecology. M.: Medicine, 1976, s.
3. Strel'nikov V.N., Dultsev J.V., Salamov KN. Surgical treatment premonicion-vaginal fistula (literature review)//Surgery. - 1980, No. 9, - s-108.
4. I. Bolshakov. et al. "A way to strengthen the sclera". The application for the invention, And 61 To 31/722, priority from 01.07.04 # 2004120228 (021704).
5. I. Bolshakov. et al. "A method of obtaining an artificial matrix of the skin". RF patent №2252787 from 27.05.2005. Bull. No. 15 dated 27.05.2005.
6. I. Bolshakov. et al. "Wound covering based on collenchymatous complex". A positive decision on the application for the invention. Priority No. 2003130390 from 4.10.2003.
7. I. Bolshakov. et al. Antibacterial filler for sanitary towels or tampons "HMD-BOL, the method of its production and use. The application for the invention. A 61 L 13/28. Priority No. 2005114248 (016342) dated 11.05.2005.
The method of treatment of genital fistula, including the treatment of the vaginal walls and the introduction of fistulous turn Antisept the ka, characterized in that the fistulous course impose a 3.5%gel of chitosan ascorbate, containing metronidazole at a dose of 2 mg in 1 ml in volume to 20 ml of 1 every 2 days until complete closure of the fistula.
FIELD: medicine, surgery.
SUBSTANCE: one should apply a polycompositional film onto donor's wounds after autodermoplasty performed. This film contains the following components in weight proportions: chitosan 78.3-89.4; polyvinyl alcohol 9.8-19.8; antibiotic of aminoglycoside group 0.5-2.0; anesthetic 0.1-0.2. It is perforated at tension coefficient being 1:4. The innovation enables to decrease wound's traumatization, improves prophylaxis of suppuration and increases cosmetic effect even at a single application of the film suggested.
EFFECT: higher efficiency of therapy.
SUBSTANCE: the present innovation deals with application of pleuromutilin derivatives, that is valnemulin and thiamulin, for transdermal treatment of bacterial diseases, in particular those induced by Dichelobacter nodosus, Fusobacterium necrophorum, Bacteriodes nodosus and Bacteriodes melamnogenicus, for manufacturing medicinal preparation or as an active ingredient of medicinal preparation of the same indication and corresponding method for transdermal treatment of diseases, for example foot rot. It has been detected the capacity of antibiotics to penetrate skin and enter either plasma or blood at concentrations being efficient against systemic bacterial infections, so, medicinal preparation could be designed in the form of ointment, cream, solution, shampoo, powder and spray.
EFFECT: higher efficiency of application.
9 cl, 1 tbl
FIELD: biotechnology, immunology.
SUBSTANCE: invention proposes preparation that comprises the immunoelectrophoretically pure secretory immunoglobulin A isolated from whey milk and/or colostrum of immunized ungulate animals and pharmaceutically acceptable vehicles. The base preparation (substance) comprises 6-12% of secretory immunoglobulin A at pH 4-8, an anti-complementary activity at least 10 mg of protein, not activating 2 CH50, protects in >70% against corresponding infections (in infection macroorganism in doses ≥10 ID50), shows areactogenic property in intravenous administration, can comprise stabilizing additives in the total concentration 4%, not above. The preparation possesses high purity, low anti-complementary activity, stable in storage, useful for oral, parenteral and topical using and possesses therapeutic activity with respect to microorganisms and viruses against which humans and animals immunization have been carried out. Invention can be used in treatment and prophylaxis of immunodeficiency states, bacterial and viral infections in humans and animals.
EFFECT: valuable medicinal and veterinary properties of preparation.
9 cl, 1 tbl, 10 ex
FIELD: organic chemistry, chemical technology, medicine, pharmacy.
SUBSTANCE: invention relates to novel heterocyclic compounds comprising 2-aminopyridin-3-sulfonic fragment of the general formula (1) or their pharmaceutically acceptable salts, N-oxides or hydrates possessing properties of antagonists of glutamate-induced calcium ions transport, in particular, neuroprotective effect. Also, invention relates to the focused library for the search of biologically active leader-compounds comprising at least one heterocyclic compound of the general formula (1) and to pharmaceutical composition if form of tablets, capsules or injections placed into pharmaceutically acceptable package containing compounds of invention as an active substance. In compound of the general formula (1) R1 represents hydrogen atom; R2 represents chlorine atom, optionally substituted hydroxyl group, optionally substituted amino-group, optionally substituted azaheterocyclyl; or R1 and R2 in common with nitrogen and sulfur atoms to which they are bound form optionally substituted and optionally condensed with other cycles 1,1-dioxo-4H-pyrido[2,3-e][1,2,4]thiadiazine or optionally substituted and optionally condensed with other cycles 5,5-dioxo-5,6,7,9-tetrahydro-5-thia-1,6,9-triazabenzocyclohepten-8-one. Also, invention discloses methods for preparing different compounds of the general formula (1).
EFFECT: improved preparing methods, valuable medicinal properties of compounds.
10 cl, 4 sch, 4 tbl, 9 ex
FIELD: organic chemistry, biochemistry, pharmacy.
SUBSTANCE: invention relates to new heterocyclylsulfonyl alkylcarboxylic acids and their derivatives of the general formula (1): or their pharmaceutically acceptable salts, N-oxides or hydrates possessing the inhibitory effect on kinase activity and to the focused library for search of active leader-compounds comprising at least abovementioned compound. In the general formula 91) W represents optionally substituted heterocyclic radical, among them: pyrrole-3-yl, thiophene-2-yl, isooxazole-4-yl, pyrazole-4-yl, imidazole-4-yl, pyridine-3-yl, 1H-2,4-dioxopyrimidine-5-yl, 2,3-dihydro-1H-indole-5-yl, 2,3-dihydro-1H-indole-7-yl, 1,3-dihydro-2-oxoindole-5-yl, 2,3-dioxo-1H-indole-5-yl, 2-oxo-3H-benzoxazole-6-yl, benzothiazole-6-yl, 1H-benzimidazole-5-yl, benzo[1,2,5]oxadiazole-4-yl, benzo[1,2,5]thiadiazole-4-yl, 1,2,3,4-tetrahydroquinoline-6-yl, 3,4-dihydro-2-oxo-1H-quinoline-6-yl, quinoline-8-yl, 1,4-dihydro-2,3-dioxoquinoxaline-6-yl, 3-oxo-4H-benzo[1,4]oxazine-7-yl, 3-oxo-4H-benzo[1,4]thiazine-7-yl, 2,4-dioxo-1H-quinazoline-6-yl, 2,4-dioxo-1,5-dihydrobenzo[b][1,4]diazepine-7-yl or 2,5-dioxo-3,4-dihydrobenzo[b][1,4]diazepine-7-yl; Y represents optionally substituted methylene group; R1 represents chlorine atom, optionally substituted hydroxyl group, optionally substituted amino-group, optionally substituted azaheterocyclyl; n = 1, 2 or 3; or Yn represents carbon atom of optionally substituted (C3-C7)-cycloalkyl or optionally substituted (C4-C7)-heterocyclyl. Also, invention relates to a pharmaceutical composition in form of tablets, capsules or injections placed into pharmaceutically acceptable package.
EFFECT: valuable properties of compounds.
5 cl, 3 sch, 5 tbl, 6 ex
FIELD: organic chemistry, chemical technology, antibiotics.
SUBSTANCE: derivative of 9-deoxo-9a-aza-9a-homoerythromycin A of the formula (3) wherein R4 represents hydroxyl protecting group is prepared by protection of 2'-hydroxy-group of compound of the formula (5) to form compound of the formula (4)
and by oxidation of C-4''-hydroxy-group of compound of the formula (4) that is carried out by addition of dimethylsulfoxide to solution containing compound of the formula (4) and a solvent followed by cooling the mixture up to about -70°C, activation of dimethylsulfoxide in situ and defoaming the reaction mixture. Compound of the formula (4) is converted to the oxidation stage directly without its isolation. Also, invention proposes additive salt of trifluoroacetic acid of compound of the formula (3) and a method for its preparing by treatment of compound of the formula (3) with trifluoroacetic acid. Invention provides increasing yield and improving purity of the end product.
EFFECT: improved preparing method.
11 cl, 6 ex
SUBSTANCE: the present innovation deals with infecting macrophages and cells of macrophagous cell lines with pathogenic microorganisms, their treatment with medicinal remedies followed by gamma-irradiation, immunization of disease-resistant and disease-sensitive animal lines and infecting vaccinated animals with alive pathogenic microorganisms that leads to improved immunity to pathogenic microorganisms.
EFFECT: higher efficiency.
4 cl, 1 dwg, 3 ex
FIELD: medicine, gynecology.
SUBSTANCE: one should perform laparoscopy, drain purulent foci, remove destructive tissues and wash abdominal cavity with great amount of "Baliz-2" introduced into abdominal cavity by fractional portions per 1-1.5 l at exposure up to 3 min either once or up to three times. The method provides complex impact as antibacterial, antioxidant and immunocorrecting actions of the preparation in area of lesion and, thus, quick interruption of acute stage by preventing the development of adhesions and saving reproductive function in this category of patients.
EFFECT: higher efficiency of therapy.
FIELD: biotechnology, microbiology, medicine, veterinary science.
SUBSTANCE: for preparing vaccine toxigenic strains of S. dysenteriae R-forms are grown, cells are subjected for lysis by treatment with chloroform, mixture is centrifuged and prepared supernatant is treated with saturated monobasic carboxylic acid or their derivatives and pH value is brought about to 3.0-5.0. Mixture if centrifuged and precipitate containing corpuscular antigens and shigellosis exotoxin are obtained. Precipitate is dissolved in buffer and pH value is brought about to 7.5-9.0. Then formalin is added in the amount 0.4-0.8% of the solution volume, or benzoic acid or benzoic acid salts are added in the amount 0.07-4.0% of the solution volume, or a mixture consisting of formalin and benzoic acid or benzoic acid salt solutions in the amount 0.10.3% and 0.03-2.5% of the solution volume, respectively. The solution is kept at temperature 30-60°C for 2 h - 60 days to provide the conversion of exotoxin to anatoxin and vaccine is prepared. Another variant of the claimed invention involves additional treatment with formalin or benzoic acid or benzoic acid salts to provide conversion of exotoxin to anatoxin, vaccine is prepared followed by its bagging and corking. For preparing immunoglobulin preparation animals are immunized with vaccine prepared by abovementioned methods followed by taking off blood, milk and/or colostrums, and/or blood, immunoglobulin fraction is prepared, sterilized, bagged and corked. This preparation is a component of the immunobiological preparation. The immunobiological preparation comprises the immunoglobulin preparation and at least one component taken among the following row: human and/or animal immunoglobulin preparations, lactoferrin, enzymes, inhibitors of proteolytic enzymes, human and/or animal normoflora preparations, yeast, vitamins, vitamin-like substances, human and/or animal proteins of acute phase, human and/or animal cytokines, higher plants components, lower plants components, components of natural origin products, apiculture products, enterosorbents, antibiotics, antibacterial chemopreparations, sulfanilamide drugs, antibacterial, anti-tuberculosis, antiviral preparations, antifungal antibiotics, synthetic antifungal preparations, stimulators of metabolic processes, antioxidants, mineral supplements, carbohydrates, lipids, replaceable and/or essential amino acids, organic acids, alkaloids, glycosides, taste supplements, aromatic supplements, base for suppositories, base for ointment formulations, technological additives for tableting, or their mixture. Invention provides preparing preparations eliciting antigenic activity with respect to broad species of pathogenic and opportunistic gram-negative microorganisms of intestine group and their exotoxins and therefore eliciting with prophylactic and curative effect with respect to diseases causing with these microorganisms.
EFFECT: improved preparing method, valuable properties of vaccine.
13 cl, 112 ex
FIELD: biotechnology, microbiology.
SUBSTANCE: invention proposes applying Flavobacterium odoratum culture as an antibacterial agent isolated from drinking mineral water "Ust-Kachkinskaya" from the hole 1/99. Microorganism Flavobacterium odoratum inhibits growth of Staphylococcus aureus, colon bacillus and yeast-like fungi Candida albicans that allows using microorganism Flavobacterium odoratum as an antibacterial agent. Invention can be used as an antibacterial agent.
EFFECT: valuable medicinal properties of agent.
SUBSTANCE: method is accomplished in the following order. First, daily sanations of vagina with 0.06% sodium hypochlorite solution are fulfilled for 10 min, total number of procedures being 5. If clinical-laboratory symptoms of inflammation disappear, eubiotics are then introduced intravaginally. In case of persistence of clinical-laboratory symptoms of inflammation, dosage forms with antimicrobial agents are introduced intravaginally or introduction of the latter is combined with oral administration of antibacterial preparations followed by intravaginal introduction of eubiotics.
EFFECT: accelerated immunocorrection leading to reduced treatment course to combined effect of sodium hypochlorite, antibiotics, and eubiotics.
FIELD: medicine, gynecology, pharmacy.
SUBSTANCE: invention proposes using pimafucin (natamycin) as agent for treatment of bacterial vaginitis. Method for treatment of bacterial vaginitis involves intravaginal administration of pimafucin as 2% cream, 2 times per a day in morning and evening for 7-10 days and for first 3 days pimafucin-containing vaginal suppository in the dose 100 mg is administrated additionally in evening after administration of cream into vagina. Invention provides high effectiveness of treatment and clinical-etiological recovery in 92.3% of cases being without prescription of medicinal preparations. Method has no contraindications and can be used in all period of pregnancy and without adverse effects.
EFFECT: improved treatment method, enhanced effectiveness of agent and treatment.
2 cl, 1 ex
FIELD: medicine, gynecology.
SUBSTANCE: method involves using the following complex of medicinal agents: vaginal suppositories Hexicon, 1 suppository in the morning and night; Multitabs-maxi, 1 capsule, once per a day for 1 month; Eleutherococcus senticosus plant extract, 30 drops in the morning and in the daytime, before eating, for 2 months; Rokicen-RD, 1 spoon in the morning and night, 30 min before eating for 10 days and repeating this course of Rokicen-RD in 10 days. Invention promotes to arresting exacerbation disease for the shortest time on the background of absence of adverse effects and iatrogenic complications. Invention can be used for treatment of vaginal candidosis.
EFFECT: enhanced effectiveness of treatment.
FIELD: veterinary medicine.
SUBSTANCE: poultry's diet during the first and second phases of egg yield should be supplemented with roxazym enzymatic complex at the ratio towards husk-bearing components being 0.007 - 0.009 : 20. The present innovation prevents inflammation of oviducal vagina in laying hens.
EFFECT: higher efficiency of prophylaxis.
SUBSTANCE: method involves administering selective modulator of steroid sex hormones being in particular compounds of general formula(I) and some quantity of steroid sex hormones precursor selected from a group composed from dehydroepiandrosterone, dehydroepiandrosterone sulfate, androst-5-en-3β,17β-diol and compounds in vivo transformable into one of cited precursors. Bisphosphonates combined with selective estrogen receptor modulators and/or steroid sex hormones precursor are additionally introduced for medically treating and/or inhibiting osteoporosis progress.
EFFECT: enhanced effectiveness of treatment; excluded adverse side effects.
41 cl, 13 dwg, 4 tbl
FIELD: medicine, dermatology.
SUBSTANCE: invention proposes an anti-infectious preparation comprising the combination of active substances with topical and systemic antifungal agents and a water-insoluble film-forming agent. The systemic antifungal agent is taken among the group including intraconazole, terbinafine and fluconazole or their salts. The topical antifungal agent is taken among the group including ciclopirox, 6-(2,4,4-trimethylpentyl)-1-hydroxy-4-methyl-2(1H)-pyridone, amorolfine and butenafine or their salts. The preparation is used as lacquer for nails in therapy of onychomycosis. The lacquer preparation provides high concentration of systemic antifungal agents in nails after its topical applying. The significant advantage of the preparation involves short time in treatment of anychomycosis.
EFFECT: enhanced effectiveness and valuable medicinal properties of preparation.
6 cl, 6 ex