The way waste complex processing of chitin-containing raw material

 

The invention relates to a method for integrated processing of chitin-containing raw materials with the aim of obtaining chitin/chitosan and enzymatic protein hydrolysates for use as the basis of microbiological culture media. Carry out the enzymatic hydrolysis of proteins chitin-containing waste under the action of a proteolytic enzyme secreted by the liver - raw crab or enzyme preparation extracted from it. The resulting solution was hydrolyzed purified by coprecipitation of lipids and insoluble substances by chitosan. The purified protein hydrolysate is dried. Upon receipt of chitin first deproteinization is carried out at a temperature of not more than 20oC. Dezazetilirovanie chitin spend 20-60% sodium hydroxide solution at a ratio of the sodium hydroxide solution : wet chitin (20-60):1 and a temperature of 95-120oWith periodic stirring, and the beginning of the process dezazetilirovanie for 5 minutes is carried out in vacuum at a pressure of 0.5-1.0 kg/cm2. The present invention allows to dispose of waste harvesting and processing of shellfish - shrimp and crabs, hepatopancreas crab, while receiving chitin, chitosan and enzymatic protein g is internal waters protein products after receipt of chitin. 5 table.

The invention relates to methods of integrated waste fishing crustaceans, including substandard shrimp, found in the by-catch of non-target crustacean species, waste crabs, shrimps and other crustaceans, with the aim of obtaining chitin, chitosan and enzymatic protein hydrolysates for use as the basis of microbiological culture media.

A method of obtaining an enzymatic protein hydrolysates for microbiological environments from non-edible organs and tissues of pinnipeds obtained under the action of this raw material sidezym enzymatic bodies of animals of the same species, for example the pancreas seals (U.S. Pat. 1402616 Russia, MKI412 N 1/20. The method of obtaining basics of nutrient media for cultivation of microorganisms/ E. S. Girshovich, N. In.Kozlov, G. A. Gerasimov and others - 4196453/28; Appl. 19.12.86; Publ. 15.06.88, bull. 22). According to this invention as a protein-containing raw materials use of non-edible organs and muscle tissues of pinnipeds, mainly seals with fetal and secondary hair. These raw materials are crushed together with the bones, pour water (1:2), is heated and set pH 8.0. is rolisa collagen. The use of raw materials together with bones in addition to improving quality * eliminates time-consuming stage of separation of the meat from the bones and increases the yield of the target product at the expense of non-waste use of meat. Then add the source of the enzymes using sidezym enzymatic bodies of animals of the same species, such as beef pancreas of Belek. Used in the way such sidezym enzymatic bodies pinnipeds, as the intestines, stomach, pancreas, are unusable waste hunting. The hydrolysis is carried out at 50oWith in 24 hours. The resulting hydrolysate is filtered, acidified with acetic acid to a pH of 5.4, boiled, filtered and dried in the spray dryer.

One of the closest to the invention of technical solutions is a method for the enzymatic protein hydrolysates from different raw protein under the action of proteolytic complex from hepatopancreas crabs (U.S. Pat. 2039460 Russia, MKI623 J 3/00. A method of obtaining a protein hydrolysate/ artukov A. A., Kozlovskaya, E. P., Kozlowski, A. S. and others - 93031307/13; Appl. 09.06.93; Publ. 20.07.95, bull. 20). Enzymatic hydrolysis of raw materials of different origin (vegetable, milk proteins, animal proteins, soy is Archi seafood, collagen, gelatin, albumin and hemoglobin in the blood) is carried out at neutral or slightly alkaline conditions (pH 8) in the presence of proteolytic complex from hepatopancreas commercial crab. Hydrolysis of conduct for 5-8 hours at a temperature of 37oWith, then spend thermoinactivation enzyme preparation at 95-100oWith subsequent cooling.

Closest to the invention, the technical solution is the way waste complex processing of chitin-containing raw material (U.S. Pat. 2123269 Russia, MCI AND 23 L 1/33, AND 23 J 1/04, 08 AT 37/08. The way waste complex processing of chitin-containing raw material/ Levenkov S. C., Bush N.M., Pancakes Y., - Appl. 24.09.97 - 97 115965/13; Publ. 20.12.98, bull. 35). The method involves the extrusion of raw materials with the separation of protein fractions, complex chitina and proteolytic enzymes and fat with getting the protein product of the pulp, washing the pulp with water at a ratio of pulp and water is 1:2 and the temperature not exceeding 25oWith his deproteinizovana, demineralization by treatment with hydrochloric acid, followed by separation on chitin and acid hydrolyzed, washing chitin until neutral with water, the purification of chitin from proteins by treatment with alkali solution, followed by the separation of deleteproduct, mixing, combining acid hydrolysates obtained at the stage of demineralization, and alkaline hydrolysates obtained during purification of chitin, to ensure a neutral pH, followed by the separation of mineral sediment protein, characterized in that is used as raw material nettervibration JI crab production, the liquid fraction after pressing the raw material is subjected to autolysis within 2-6 hours at a temperature not higher than the 12oC, centrifuged to obtain a protein paste, grease and solution of proteolytic enzymes, are Stripping the fat and solution of proteolytic enzymes by separation and filtration through a suction filter obtaining a complex of proteolytic enzymes and fat-prefabricated, deproteinizovana pulp after washing with water is carried out in a period of 1-24 h obtained by the solution of a complex of proteolytic enzymes with pH 7.5-8.0 and containing 1-10% of the enzyme preparation to the mass of raw material with a proteolytic activity of the drug 60-200 PE/g at a temperature of 4-60oAnd the ratio of raw material : solution 1:1-1:1.5 Department of protein hydrolysate, the remaining pulp is treated with vegetable oil to obtain an oil extract of carotenoid pigments, and receive further washing it with water, spend the purification of proteins with the final product of chitin. Purification of chitin from proteins spend 2.5 to 4% alkali at a temperature of 55-65oWith over 2.0 to 2.5 h at a ratio of chitin : a solution of 1:10-1:5.

There is also known a method of extraction of chitin, including the enzymatic deproteinization shells of crustaceans (Rogozhin, S. C., Lozinsky Century. And., Fokin S. C., Eremenko L. I., Gamzazade A. I., Tsarapkin C. A.; Institute of Organoelement compounds, USSR Academy of Sciences. Isolation of chitin aquatic organisms: A. C. 1022463 the USSR, MKI 08 In 37/08. Isolation of chitin hydrobionts/Lozinski Century. And., Fokin S. C., Oresence L. I., Gamzazade A. I., Tsarapkin Century A. 3351422/23/05; Appl. 19.10.81).

Isolation of chitin hydrobionts, including the enzymatic deproteinization (DP) and the acid of decalcomanie (DK) armor, characterized in that for purposes of simplification and cheapening of the process, as well as reducing the amount of polluting waste, DP and DK are at the same time handling shells in an aqueous solution of acidic proteolytic enzymes of microbiological origin at pH 1.5 to 4.5 and 15-60oWith 1-48 h with a ratio of enzyme: substrate armor 1:1-1:1000. As acidic proteolytic enzymes of microbiological origin use sour is llus oryzae.

A method of obtaining chitosan, which is patented reuse of alkali to dezazetilirovanie chitin (Patent 2087483 Russia, MCI 08 IN 37/08, 01 J 20/3O. A method of producing chitosan/ Owl centuries, Freiman D. B. , Bannikov centuries , L. F. I. - 93055356/25.-Appl. 21.12.93; Publ. 20.08.97, bull. 23).

A method of producing chitosan, including grinding of natural chitin-containing raw material, decalcomania 1-5% solution of hydrochloric acid, the deproteinization 1-5% sodium hydroxide solution, washing and deacetylation 46-47% sodium hydroxide solution at 80-85oAnd the Department of chitosan from the mother liquor, wherein the chitin-containing raw materials are crushed, then before washing spend three cycles of alternating stages of deproteinization, decalcomania, the deproteinization is carried out at 60-85oC for 1-3 h, decalcomania when 35-55oC for 0.5-2 h, and each subsequent cycle of alternating stages is carried out at a higher temperature than the previous one. The mother liquor from the stage of deacetylation concentrate and serves on the stage of deacetylation.

The present invention expands the resource base for the production of chitin, chitosan and enzymatic baramula and processing of crustaceans, as well as the use of raw hepatopancreas crab or enzyme preparation derived from it, as a highly active enzyme preparation of a wide range of proteolytic and collagenolytic activity.

Before receiving from chitin-containing raw material of chitin/chitosan proposed method provides a preliminary enzymatic hydrolysis of proteins wastes shrimp and crab fishing under hepatopancreas raw crab or proteolytic enzyme preparation allocated from him, at a ratio of enzyme/liver-raw : the protein-containing raw material waste crustaceans fishery, as 1-10/10-200 g : 1 kg

The resulting hydrolysate is purified by centrifugation and degrease using coagulant - chitosan, the residue is again separated by centrifugation. The obtained clear solution of the hydrolyzate is evaporated under vacuum and dried.

Chitin-containing precipitate (shell) washed with water, dried, or use wet for obtaining chitin and chitosan. Chitin receive consistent treatment of shell solutions of alkali (deproteinization), acid (demineralization). First deproteinization is carried out at a temperature of the temperature within 95-120oAnd the ratio of the sodium hydroxide solution : wet chitin/chitosan (20-60):1 with periodic stirring. At the beginning of this process dezazetilirovanie within 5 minutes is carried out at vacuum pressure of 0.5-1.0 kg/cm2. Treatment can be repeated 2-3 times to obtain a high degree of dezazetilirovanie.

Raw material for producing hydrolyzate are waste of fisheries shrimp (substandard shrimp, "Lom"), waste processing king crab and other crustaceans and chitin-containing waste recycling. Getting hydrolysate is part of a comprehensive waste recycling harvesting and processing of shellfish. Substandard shrimp for chitin extraction unprofitable, because the yield is about 1.5%, which is 3-4 times less than the output of chitin from the shells obtained after mechanical processing of shrimp by aerial or hydroxylysine. The use of fishery waste for feeding purposes is also ineffective, because the production of chitin economically feasible.

In this regard, we proposed previously to carry out the enzymatic hydrolysis of all the proteins separated shell to obtain a protein hydrolysate, imeushih is the remainder - chitinous carapace, which is the raw material for chitin extraction. The present invention expands the resource base for the production of chitin, chitosan and enzymatic protein hydrolysates for microbiological environments through the use of non-conforming products and waste harvesting and processing of shellfish, as well as the use of raw hepatopancreas crab or enzyme preparation derived from it, as a highly active enzyme preparation of a wide range of proteolytic and collagenolytic activity.

The use of proteolytic complex, obtained from hepatopancreas fishing Kamchatka crabs, allows to produce products different from the products obtained by the use of other enzyme preparations. This is due to the fact that the protease contained in the crab sector, have unique substrate specificity that distinguishes them from other types of proteolytic enzymes.

Thus, the use of the invention allows to solve the following tasks: - to dispose of non-previously unusable protein-containing raw materials - waste crustaceans: proteins of shrimp and crab, liver crab; - p the teachings of chitin and reduce the degree of pollution of wastewater protein products due to a preliminary enzymatic hydrolysis of raw materials to produce protein hydrolysate (pre-treatment crustacean shell from a significant portion of proteins); - production of chitin and chitosan of high quality.

The method according to this invention is as follows.

Raw thawed, crushed into a homogenizer, a grinder or other device until a homogeneous mass with a particle size of the shell from 1 to 10 mm, the Crushed raw material is loaded into the apparatus with a stirrer and a jacket for heating, pour the water, the mass ratio of raw materials and water is 1: 1. Download enzyme preparation in an amount of from 1 to 10 g per 1 kg of raw material or liver raw depending on activity from 10 to 200 g per 1 kg of raw material. The resulting mixture is stirred, heated to (505)oC. the Hydrolysis is carried out at the specified temperature for 3-10 h under stirring. After hydrolysis, the mixture is heated to 95-100oC for 10-60 min, then cooled to room temperature and the precipitate is separated by centrifugation.

The resulting solution of the hydrolyzate with a pH of about 8.5 is acidified under stirring to a pH of not more than 6.5 solution of hydrochloric acid with a concentration of hydrogen chloride from 5 to 25%.

Chitin-containing precipitate is washed with water 2-3 times, dried or immediately used to produce chitin. Dry the precipitate contains residual proteins 25-40%, ash 35-50%, hit the iza from chitin-containing waste generated after mechanical peeling shrimp, first of all, higher mass fractions of chitin. In addition, after enzymatic hydrolysis in the shell is significantly reduced fat content (table. 1).

In the solution of the hydrolyzate add the coagulant solution of chitosan in the 0.05 to 0.2 N. the solution, kept under stirring for 10 to 60 min, then the mixture is neutralized to pH values from 7.5 to 9.0 and incubated under stirring until the formation of sludge within 10-60 minutes, the resulting suspension is centrifuged or filtered. After separation of the precipitate, the pH of the solution of the hydrolyzate regulate the addition of hydrochloric acid to a value of 7.20,3.

A clear solution of the hydrolyzate evaporated under vacuum until the mass fraction of dry substances from 30 to 65%. One stripped off the solution is dried in a dryer of any structure to a residual mass fraction of water is not more than 4%. When using a spray dryer or dryer with inert carrier in a fluidized layer solution of the hydrolyzate is allowed to dry without prior evaporation.

The obtained dry hydrolyzate is soluble in water to a concentration of 3-5%. Mass fraction of fat from 0.3 to 0.8%. The output from 10% to 15%.

To obtain chitin/chitosan wet shell is loaded into the reactor and pour the sodium hydroxide solution with concinna or periodic stirring. After the first deproteinization suspension is directed to the separation of the precipitate by centrifugation (preferably sedimentation or filtration. The liquid part is directed to the deposition of proteins, deproteinizing shell washed with water until neutral pH of the wash water and sent for demineralization.

The demineralization spend a 3.6% solution of hydrochloric acid at a temperature of 15-25oC for 0.5 to 2 hours at a constant or periodic stirring. After demineralization, the suspension is sent to the sludge separation by centrifugation or filtration. The liquid part is directed to the deposition of mineral salts, demineralized shell washed with water until neutral pH of the wash water and directed to the second deproteinization.

The second deproteinization spend 4% sodium hydroxide solution at a temperature of 90-98oC for 0.5 to 2 hours at a constant or periodic stirring. After the second deproteinization suspension is directed to the separation of the precipitate by centrifugation or filtration. The liquid part is neutralized and plums, chitin was washed with water until neutral pH of the wash water and sent for drying or production of chitosan.

From Asia hydrochloric acid until the pH of the solution is not more than 5.5. The precipitate was separated by centrifugation or by filtration, dried and used as an additive in animal feed. The solution is drained.

From acid solution after demineralization conduct mineral deposition of calcium salts by neutralization with a saturated solution of calcium carbonate to a pH of at least 9. The precipitate was separated by centrifugation or by filtration, dried and used for feed or industrial purposes. The solution is drained.

Qualitative indicators of chitin reflected in the table.2 examples of the method.

Chitin is used to obtain chitosan or glucosamine hydrochloride.

Chitosan is obtained from the dried and wet chitin.

Dezazetilirovanie dried chitin spend 20-60% sodium hydroxide solution at a temperature of 95-120oC for 30-90 min at a constant or periodic stirring. To improve the hydrodynamic reaction conditions of dezazetilirovanie and conditions of the regeneration of caustic soda weight ratio of sodium hydroxide solution and chitin (when re - treatments-chitosan) was increased and ranges from 20:1 to 60:1. After the process is finished, the solution is separated by filtration or centrifugation and, after the addition of the necessary quantity of school is the temperature 85-98oWith up to a neutral pH value of wash water, dried, or use wet for a deeper dezazetilirovanie. The second and third dezazetilirovanie carried out similar to the first. Get the chitosan with a degree of dezazetilirovanie from 65 to 99%, depending on the multiplicity of treatments with sodium hydroxide solution and the duration of each of the treatments.

To exclude the operations of drying the intermediate product (chitin and chitosan) is dezazetilirovanie wet chitin/chitosan. In this case, pre-prepared when heated to 80-90oWith the alkali solution with a concentration of 20-60%, taking into account the mass fraction of water in the wet chitin/chitosan. In table.2 shows the parameters of solutions of alkali required to dezazetilirovanie 100 kg wet chitin, with a mass fraction of water 75%.

As can be seen from the table.2, when the ratio of 10:1 and 15:1, which is usually used for dezazetilirovanie, when using wet chitin will require the preparation of a solution of NaOH with a concentration above 60%, which can cause difficulties even during short-term storage of this solution due to the rapid crystallization of alkali. At concentrations above 70% will require temperatures above 100oC. Adding wet chitin in restorm, we came to the conclusion that when dezazetilirovanie wet chitin should increase the ratio of alkali solution and chitin more than 20: 1. It has several positive aspects. At high ratios is more uniform mixing of the reaction mixture, resulting in a more homogeneous product. When regeneration of the alkali solution dissolving an additional amount of NaOH is easier, as changes in the concentration of the solution is from 2.5 to 5.5%, and the solution does not require intensive heating for short storage.

In order to intensify the processes of deproteinization and demineralization shell, dezazetilirovanie chitin/chitosan is offered after loading all reagents and raw materials to carry out short-term evacuation of the reaction mixture. When the vacuum is the boiling of water in the pores of the wet products (shell, intermediate products, wet chitin/chitosan), which after the restoration of atmospheric pressure in the pores easily filled with the reagent solution. The most effective short-term evacuation when dezazetilirovanie wet chitin/chitosan. Uniform rapid absorption of 20-60% NaOH solution provides for the those substances (see table.5 for example 4).

The proposed method integrated waste harvesting and processing of shellfish has the following advantages: - the implementation of integrated use of raw materials, which does not require prior separation of its components, in particular the separation of the shell, and used almost all of the waste; - the use of hepatopancreas raw crab or, if you need production, enzyme preparation derived from it, allows for efficient enzymatic hydrolysis of proteins. As a result, high mass fraction of the target product, namely amino acids and lower peptides in protein hydrolysates. The high content of free amino acids in the hydrolysate, largely characterize the depth of enzymatic hydrolysis, is because you used the integrated product proteases from hepatopancreas crab, which has high affinity to the tissues of crustaceans included in the diet of crab; - simplification of the purification process of the solution of the hydrolyzate from the ballast insoluble proteins and lipids through the use of effective coagulant organic substances - chitosan; - PR is Abadi and obtain chitosan) with a lower content of proteins and lipids in comparison with shell after mechanical peeling; - is deposition of protein and minerals with high outputs separately from the alkaline and acid solutions after the production of chitin;
- going process optimization dezazetilirovanie chitin/chitosan due to the increase in the ratio of 20-60 mass% sodium hydroxide solution and wet chitin/chitosan, which allows to improve the hydrodynamic conditions of the reaction dezazetilirovanie and conditions of the regeneration of the caustic soda solution at dezazetilirovanie;
- obtaining a homogeneous quality product at the expense of short-term degassing of the reaction mixture at the beginning of dezazetilirovanie that provides fast wetting wet chitin/chitosan solution of alkali.

Example 1.

Production of chitin and enzymatic protein hydrolysate from the waste of the shrimp fishing by using enzyme preparation from hepatopancreas.

2.5 kg cooked frozen wastes fishery shrimp thawed in air at room temperature, grind on the homogenizer until a homogeneous mass with a particle size of shell is not more than 3 mm, the Crushed raw materials were loaded into a flask with a capacity of 10 l with a mixing device. Filled with 2.5 kg of water. Downloaded 18 g of dry fermented (505)oC. the Hydrolysis was carried out at a temperature of (505)oC for 6 hours After completion of the hydrolysis, the temperature in a water bath brought to 97-98oWith and for warming the reaction mixture in the flask for 45 minutes the mixture is Then cooled to room temperature. The precipitate was separated by centrifugation at 8000 rpm for 30 minutes

Received of 3.69 kg of a solution of the hydrolyzate with a pH of 8.5. The resulting solution was neutralized 18% hydrochloric acid to pH 6.5. In the solution under stirring was added 350 g of a 1% solution of chitosan in 0.1 N. hydrochloric acid, the mixture stood at periodic stirring 20 min, then neutralized to a pH of 8.25 adding a 20% aqueous solution of sodium hydroxide and passed with periodic stirring for 30 minutes the Suspension was centrifuged at 8000 rpm for 30 minutes

The obtained clear solution of the hydrolyzate was evaporated under vacuum on a rotary evaporator IL-10M to the mass fraction of dry substances of 47%. One stripped off, the solution was dried in a vacuum drying Cabinet at a temperature of not more than 80oC. the Dry hydrolysate to grind.

Received 251 g of dry hydrolysate, completely soluble in water to a concentration of 5%. Output 10%. Chemical composition and aminocyclo 125 g or 5% in terms of and.with.in.). The chemical composition of the dried sample shell shrimp are shown in table.3.

In a flask with a capacity of 3 l was downloaded 1.5 l of 4% aqueous sodium hydroxide solution with a temperature of 20oC and 600 g of wet shell shrimp. The mixture was mixed for 1 h and the precipitate was separated by centrifugation at 8000 rpm for 30 minutes and Then the precipitate deproteinizing shell washed on the filter with water with a temperature of 80-90oWith until pH of the filtrate is not more than 8,0.

In a flask with a capacity of 3 l was downloaded 1.5 l of 3.6%-aqueous solution of hydrochloric acid at a temperature of 20oAnd deproteinizing shell shrimp. The mixture was mixed for 30 min and demineralized shell separated by filtration and washed on the filter with water temperature 15oWith until pH of the filtrate is not less than 5.0.

For complete removal of proteins and discoloration of chitin conducted a second deproteinization sludge 4% sodium hydroxide solution at a temperature of 85-95oC for 1 h Chitin was washed with hot water until neutral and dried in a drying Cabinet at a temperature of 60oC.

Got 34 g of dry chitin. The yield of 1.36%.

The chemical composition of dried chitin are shown in table.3.

Example 2.

Production of chitin and enzymatic protein hydrolysate is different waste fishery shrimp thawed in air at room temperature, to grind on the homogenizer until a homogeneous mass with a particle size of shell is not more than 3 mm, the Crushed raw materials were loaded into a flask with a capacity of 10 l with a mixing device. Flooded 2.3 kg of water. Downloaded 200 g crushed hepatopancreas raw crab. Further hydrolysis, separation and purification of the hydrolysate was carried out according to example 1.

Got 420 g of dry hydrolysate, completely soluble in water to a concentration of 5%. The output of 16.8%. Chemical composition and amino acid composition of the hydrolysate are shown in table.3 and 4.

Received 667 g of shell shrimp with mass fraction of water 82% (Exit 120 g or 4.8% in terms of and.with.in.). The chemical composition of the dried sample shell shrimp are shown in table.3.

Production of chitin was carried out analogously to example 1.

Received 33 g of dry chitin. The yield of 1.32%.

The chemical composition of dried chitin are shown in table.3.

Example 3.

Production of chitin and enzymatic protein hydrolysate from waste king crab using hepatopancreas raw.

Enzymatic treatment was carried out similarly to example 2 using 2.5 kg of raw-frozen mussel processing waste king crab (Karabasov) and 200 g of hepatopancreas crab.

Got 180 g of dry guide is in the hydrolysate are shown in table.3 and 4.

Got 1745 shell crab with mass fraction of water 65% (Output 610 g or 24.4% in terms of and.with.in.). The chemical composition of the dried sample shell shrimp are shown in table.3.

Production of chitin is similar to example 1.

Got 135 g of dry chitin. The yield of 5.4%.

The chemical composition of dried chitin are shown in table.3.

Example 4.

Production of chitosan from wet chitin with a short-term evacuation of the reaction mixture.

In two flasks with a capacity of 3 l downloaded by 640 g of water and with stirring at 800 g of sodium hydroxide in each. Got 1440 g 55,6%-aqueous solution. In a hot solution was loaded on 200 g of chitin from crab, with a mass fraction of water 80% (example 3). The flask was closed and the second created a vacuum pressure of 0.9 kg/cm2. After boiling the mixture after 5 min in the flask was restored to atmospheric pressure and continued dezazetilirovanie under stirring at a temperature of 105oC for 30 minutes In the first bulb dezazetilirovanie conducted under the same conditions, but at atmospheric pressure.

After dezazetilirovanie the alkali solution was separated by filtration. Precipitation of chitosan was washed with water with a temperature of 80-90oWith until pH of the filtrate is not above 8.0. The washed precipitates were dried in the drying Cabinet is

Chemical composition and properties of chitosan are shown in table.5.

As can be seen, the greatest effect of the vacuum is observed when comparing figure insoluble substances, which is explained by the acceleration of the process of diffusion of the alkali solution to the internal zones of particles of particles of chitin.

The present invention allows for a single cost-effective technologies to utilize previously unused in the processing of chitin and protein-containing raw materials - waste harvesting and processing of shellfish: shrimp and crab, hepatopancreas crab, while receiving chitin/chitosan and enzymatic protein hydrolysates for microbiological culture media. It is also important that the proposed method can reduce the degree of pollution of wastewater protein products after receipt of chitin.


Claims

The way waste complex processing of chitin-containing raw material, including the production of protein hydrolysate from the feedstock enzymatic hydrolysis of chitin chitin-containing sludge from the holding of the first deproteinization, demineralization, the second deproteinization and drying of the finished product, characterized in that when receiving protein Hydra crab or complex enzyme preparation, received from him, with a ratio of 1-10/10-200 g : 1 kg of raw material, the precipitate was separated, protein hydrolysate cleaned by coprecipitation of lipids and insoluble substances chitosan, purified protein hydrolysate is dried, upon receipt of chitin first deproteinization is carried out at a temperature of not more than 20oC for 0.5 to 2.0 hours under stirring, and the second 90-98oC for 0.5 to 2.0 hours under stirring, the precipitate was separated, washed chitin and dried or direct the production of chitosan, by dezazetilirovanie wet chitin 20-60% sodium hydroxide solution at a ratio of the sodium hydroxide solution: wet chitin (20-60):1 and 95-120oWith periodic stirring for 30-90 min, and 5 min from the beginning carried out in vacuum at a pressure of 0.5-1.0 kg/cm2at the end of the process dezazetilirovanie the alkaline solution is separated, the precipitate chitosan is washed and dried.

 

Same patents:

The invention relates to biocompatible fototienda gel based on crosslinked hyaluronic acid, which has specific physical properties, the methods of its production and its applications as biomedical materials

The invention relates to the field of biochemistry and can be used in medicine
The invention relates to the dairy industry, can be used in fish, meat and food processing industry

The invention relates to medical biotechnology and can be used to create based on natural polysaccharides new radioprotectors

The invention relates to a method for producing hyaluronic acid from cocks ' combs

The invention relates to a grafted polysaccharides with antioxidants and preferably to hyaluronic acid or crosslinked hyaluronic acid grafted dull phenols

The invention relates to the chemistry of polyhedral borhydride compounds, adducts of chitosan composition (XH)2B12H12(4)Y2B12H12where X (chitosan)= C6O4H11N, Y = H+NH4+and 0x < 4, which can be used as high-calorie quickmatch component pyrotechnic materials, and to methods for their preparation

The invention relates to biochemical technologies to obtain a gel-like substance for food and medical cosmetics on the basis of chitosan

The invention relates to the food industry and can be used in the manufacture of heat-treated culinary product from the eggs of sea urchins black (Strongilocentrotus nudus) or gray (Strongilocentrotus intermedius)
The invention relates to chemical-pharmaceutical and food industry

The invention relates to the fishing industry, namely the recycling of cutting marine organisms for the production of biologically active additives to food
The invention relates to biologically active products derived from protein-containing raw water origin

Salad // 2186502
The invention relates to food industry, namely to the culinary dishes prepared from herbal ingredients with seafood
The invention relates to the food industry and relates to methods for the manufacture of salads

The invention relates to food industry, namely the production of biologically active additives to food

The invention relates to fishing and food industry, in particular to methods of processing of aquatic organisms to obtain BAS

The invention relates to food industry, namely the production of health products with the use of biologically active additives (BAA), which increases the biological value of the products
Up!