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Method of cultivation of wide row arable crops Method comprises the basic tillage, sowing, care and harvesting. And the soil tillage is carried out with chisel tool with the formation of ridge bottom of the furrow, and sowing the crop is carried out over the deepening of the furrow bottom in every second deepening in the first year of sowing. In the second year the sowing is carried out over the unused deepening of the furrow bottom of the first year, and the width of row-spacing after tillage is equal to half the distance between the rows. The direction of sowing the crop is oriented perpendicular to the motion of the dominant wind. Sowing the crop over the deepening of the furrow bottom alternates with the steam bands, which are loosened during the growing season to the depth of 0.08-0.12 m at least 2 times. After harvesting the crops the plant residues are treated with biomineral preparation of nitrogenous fertilizers, complex humic concentrate and water taken in the ratio of 5:0.2:94.8 based on 310-320 kg per hectare. |
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Dry peat-humic preparation and method for its production Invention relates to agriculture and can be used in the production of a peat-humic preparation used for root and foliar application of different crops in the open and closed ground, as well as a biologically active supplement for animals and fish. The dry peat-humic compound comprises a mixed composition on the basis of peat of preliminarily shredded peat and a biologically active organic supplement. The method of production of the dry peat-humic preparation comprises crushing, mixing and drying the organic raw material consisting of peat and the biologically active organic supplement to obtain the dry peat-humic product in the form of a homogeneous equally-dispersed mixture. The biologically active organic supplement is used as a plant composition consisting of needles of Scotch pine, Rhodiola rosea root, grass and root of anomalous peony, grass of rape and of Echinacea purpurea in the flowering stage to obtain an intermediate dry peat-humic product which is additionally crushed for 13-15 minutes, divided into four equal initial fractions, into each of which added is with stirring one dry culture of bacteria Bacillus subtilis, Bacillus licheniformis, Arthrospira fusiformis and Actinomyces. Mixing is carried out separately with each dry bacterial additive in a mixer with a water jacket, followed by chilling. Then, mixing of all received components is carried out to a homogenous mass in the mixer with a water jacket, followed by chilling the resulting mixture. Stirring is resumed to chilling to 9-11°C. The resulting ready dry peat-humic preparation containing at least 106 CFU/g dry culture of each bacterium is unloaded onto an open platform. After exposure for 24-26 hours it is packed in bags and exposed for 40-48 hours more. |
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Artificial soil and method of its preparation Group of inventions relates to agriculture. The air biosoil - an artificial soil consists of large clumps of 2-10 mm in size, uniformly distributed over the volume, the small clumps of 0.1-0.3 mm in size, and the ultrafine clumps of up to 10 microns, with a total porousness of at least 40%, with a content of organic matter of not less than 20%, the acidity of pH 6-8, having a colour from light brown to black, containing microflora characteristic to wild black soil. The method of air biosoil preparation consists in the fact that waste products of animals and other organo-mineral wastes are mixed, peat, scanty soil and other filling materials are added, the resulting mixture is crushed to obtain at least 40% of small clumps, 1-5% of mixture is selected and passed through a disintegrator to obtain ultrafine clumps, they are mixed with the rest of the mixture, the concentrated soil solution is added in an amount of 1-3% by volume of the mixture, then the sorbent additives are added to provide porousness of at least 40%, the mixture is stirred by pouring to the containers with the displaced centre of gravity to ensure homogeneity , the mixture is placed into a fixed container and maintained at an optimum temperature within 20-37°C for at least 24 hours. |
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Method of production of biofertiliser Method of production of biofertiliser comprises obtaining of bio-mixture by adding microbial cultures Pseudomonas sp. 114 deposited in the RNCIM under the number of B-5060, and Azotobacter chroococcum B-35deposited in the RNCIM under the number of B-6010, with a titre of 108 cells/ml at a ratio of 2:1 on a dry combined carrier with the rate of 60 ml per 1 kg and stirring. The carrier is used as cellulose-containing substance such as sunflower or rice husks and mineral-containing component such as perlite, taken in the ratio of 1:3 by weight, then the bio-mixture is applied to the floor of poultry premises at a dose of 30-70 g per 1 m2 at a humidity of the carrier of 15-20%, then the bio-mixture with the wastes of poultry premises is collected while accumulated and stored in piles. |
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Method of increasing soil fertility includes pre-sowing treatment of Lucerne seeds with liquid biopreparation, cultivation and mowing of lucerne green mass. For seed treatment used is liquid bacterial biopreparation based on strain Sinorhizobium meliloti Yakutskiy No 2 SRI ARRIAM RCAM00826. Said preparation is taken in amount 1 billion of cells per 1 lucerne seed. |
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Culture medium for growing consortium of nitrogen-fixing and phosphate-mobilising microorganisms Invention relates to biotechnology and can be used in microbiology. The culture medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, phosphorite and distilled water in a given ratio. |
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Culture medium for growing consortium of nitrogen-fixing and phosphate-mobilising microorganisms Invention relates to biotechnology and can be used in microbiology. The culture medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, vermiculite and water in a given ratio. |
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Culture medium for growing consortium of nitrogen-fixing and phosphate-mobilising microorganisms Invention relates to biotechnology and can be used in microbiology and agriculture. The culture medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, sapropel and water in a given ratio. |
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Culture medium for growing consortium of nitrogen-fixing and phosphate-mobilising microorganisms Invention relates to biotechnology and can be used in microbiology. The culture medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, zeolite and distilled water in a given ratio. |
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Method of production of soil mixture for germination of seeds and development of seedlings Invention relates to agriculture. The method of production of soil mixture for germination of seeds and development of seedlings, which is characterised in that the biohumus is prepared by bacterial processing of moist cellulose-containing wastes, which are used as fibre waste hydrolyzate obtained using 0.5% aqueous solution of diammonium phosphate for 20-40 minutes under pressure at a temperature of 120°C, or fibre waste, or a combination of fibre waste with crushed bark or crushed sawdust, probiotic feed additive Ferm-KM and the preparation Gliokladin™, and in the case of use of the fibre waste hydrolyzate for bacterial processing the dry fodder yeast Saccharomyces cerevisiae is additionally used, the process is carried out during a week at a temperature of 25-30°C, and the bacterial processing is carried out for a month at a temperature of 25-30°C and regular turning the mass, and the resulting product is subjected to vermicomposting with red Californian worm for 2-3 months, after that the resulting biohumus is dried and mixed with peat in a ratio of 10-30% of biohumus and 90-70% of peat, respectively. |
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Method of microbiological treatment of poultry droppings Method for microbiological treatment of poultry droppings is carried out using microbiological cultures diluted in water and introduced in poultry droppings. As the microbiological cultures the yeast strain Candida krusei-96 and food yeast Saccharomyces cerevisiae are used in a ratio of 1:1 with a titre of 108 CFU/ml. The microbiological cultures are applied in the amount of 2 ml per ton of droppings once followed by layering of poultry droppings with the adding up to 20% moisture-absorbing material. |
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Biological preparation for leguminous crop of liquid form based on nodule bacteria Biological preparation comprises the culture fluid containing the strain of nitrogen-fixing nodule bacteria with a titre of not less than 107 CFU/ml, and an aqueous solution containing (wt %): sodium carboxymethyl cellulose (2.0-5.0), potassium sorbate (0.5-3.0) and glycerol (1.0-5.0), mixed in a ratio of from 1:1 to 1:2. |
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Method of obtaining biomineral fertilisers and meliorants (versions) Invention relates to agriculture. Method of obtaining biomineral fertilisers includes combination of dry or liquid mineral fertilisers and meliorants with microbial biomass; in the first version as microbial biomass used is microbiological fertiliser or preparation, created on the basis of agronomically useful microorganisms in form of dry fine-disperse powder with titre 102-105 kl/g, which is applied on dry mineral fertiliser or meliorant or introduced into liquid mineral fertiliser in dose 2-6 kg/t; in the second version, as microbial biomass used is microbiological fertiliser or preparation, created on the basis of agronomically useful microorganisms in liquid form with titre 104-107 kl/g, which is applied on dry mineral fertiliser or meliorant by fine-disperse spraying or is introduced into liquid mineral fertiliser in dose 1-4 kg/t. |
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Method of improvement of symbiotic activity of leguminose grasses Invention relates to the field of agriculture, in particular to soil microbiology. The method comprises inoculation of seeds with crushed roots of the same species and mixing them with mineral water. At that the seeds before sowing are treated with crushed roots of leguminose grasses after the resuming of vegetation of 2-3rd year of life, wetting them with a mixture of dried after-distillery stillage and mineral water such as Tamisk in the ratio of 1:2. In the budding phase the feeding with dried stillage is carried out in the amount of 20-30 kg/ha, dissolved in water in an amount of 200-250 L/ha. |
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Method of obtaining humidified soil Method comprises the use of cellulose-containing wastes and soil, administration of microorganisms, moisturizing and incubation. At that the wastes are sorted and crushed into particles of 1-2 cm, then they are placed in layers in the fermenter, alternating with the ground, the height of each layer is 2-3 cm, and then treated with the suspension of the preparation Tamir obtained by cultivation of microorganisms without access of air at a temperature of 20-30°C with stirring once a day for 1-2 min with addition of water, sugar and the preparation of Urgas in a ratio of water: concentrate of Tamir: sugar: preparation of Urgas is 30:0.3:1:0.5. It is humified in mesophilic conditions at a temperature of 30-40°C for 120 days. |
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Method of maintenance of vineyard soil Invention relates to the field of agriculture, namely to viticulture. The method comprises initially single seeding in a row of vines on 3-year agro-technological cycles in inter-row spacing unsown in the previous cycle of winter triticale alternating in varieties cycles of selection of Krasnodar Research Institute of Agriculture n.a. Lukyanenko. Yearly in the cycles the green mass is trimmed at the stem elongation of plants in the spring. In the summer in the inter-row spacing soil the matured plant mass is embedded with ears of corn of triticale and agrobiological stimulant of effective microorganisms (EM) "Baikal EM-1" based on the special nutrient medium "EM syrup". At that together with the biomass of triticale and the stimulant EM in the inter-row spacing soil the organic matter of vegetable pulp (OP) is embedded at a rate of 380 kg/ha. |
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Method of making compost from sewage sludge using phototrophic bacteria Invention relates to public utilities and environmental biotechnology and can be used for bioconversion of sewage sludge with low content of heavy metals combined with brewery wastes - spent grains - into compost. The method involves mixing in a defined volume ratio dry sewage sludge with an organic component in form of spent grains and with a cellulose-containing component in form of saw dust, adding a compost ferment in amount of at least 15% of the volume of the mixture and subsequent aerobic composting of the mixture. After the high-temperature composting step, natural drying of the mixture which is placed in a layer of about one metre, is carried out while simultaneously adding a bioactivator in form of a liquid culture containing 109-1010 cells per ml of a suspension of anoxic purple phototrophic bacteria Rhodobacter capsulatus in volume ratio to the mixture of at least 1:50, respectively, and holding without compulsory aeration for 25-30 days, followed by stabilisation of the mixture in piles for 30-35 days. |
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Method of disposing cellulose-containing wastes Invention relates to the field of biotechnology, particularly, to a method of disposing the cellulose-containing wastes. The method of disposing the cellulose-containing wastes by using vermiculture with earthworms Eisenia fetida and culture of fungi Trichoderma viride comprises a preliminary oral inoculation of earthworm Eisenia fetida with using an insulin syringe with a sawed-off point of the needle with the suspension of mycelium and spores of a cellulose-digesting strain of week-old culture of fungi Trichoderma viride with the content in it of colony-forming units in the amount of 103 CFU/ml in amount of 0.05 ml, or daily exposure of the worms in the substrate saturated with such suspension. |
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Strain of nodule bacteria bradyrhizobium japonicum 859 for production of fertiliser for soya Strain of nodule bacteria Bradyrhizobium japonicum 859 (VNIISHM No.24117) is proposed, used as a facility to produce a fertiliser for soya. When soya seeds are treated with the fertiliser produced on the basis of the specified strain Bradyrhizobium japonicum, the index of germination increases by more than 8%, and crop capacity increases by 17-30%. |
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Method of growing soyabean with use of polycomponent fertiliser "bioplant flora" Invention relates to the field of agriculture. The method of cultivation of soyabean using polycomponent fertiliser "Bioplant Flora", including presowing treatment of seeds with rizotorfin. The presowing treatment with rizotorfin is performed in the mixture with polycomponent fertiliser "Bioplant Flora" at a dose of 1 l/t. At that foliar fertilising of plants is carried out with the polycomponent fertiliser "Bioplant Flora" in the phase of 4-5 leaves and end of bean-formation at a dose of 0.25-1 l/ha. |
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Method of treating organic wastes Invention relates to agriculture. The method of treating organic wastes using vermiculture with Eisenia fetida earthworms, wherein a strain of Trichoderma asperellum MG-97 (VKPM F-765) in form of spores or mycelium or a preparation of trichodermin M are simultaneously added with the earthworms to the starting substrate in amount of 105-106 CFU/kg of the starting substrate. |
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Bacterial fertiliser contains a humin-containing base, a bacterial additive and dry alkali. As a humin-containing base, peat or brown coal is used, or a mixture of peat and brown coal. Bacterial additive is a dry culture of bacteria Bacillus subtilis, or a mixture of bacteria Bacillus subtilis and Bacillus licheniformis. Components are taken at a specific ratio. |
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Bacterial strain Azotobacter chroococcum 5 V(e) is selected from light-grey forest soil of village Raifa of Zelenodolsk district of the Republic of Tatarstan and is deposited in RNCIM under the number B-10387. The strain synthesizes vitamins of group B and indolyl-3-acetic acid. The strain has nitrogen-fixing activity 515.7 nM N2/ml·h. |
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Fertiliser-pesticide mixture for applying under cereal crops Invention relates to agriculture. Fertiliser-pesticide mixture for cereal crops contains liquid nitrogen fertilisers (UAN-32), water, cowboy super and kinmix with the following component ratio, wt %: (UAN-32) - 30.0-40.0; cowboy super - 0.11-0.20; kinmix - 0.2-0.5, water is the rest up to 100. |
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Fertiliser-herbicide mixture for application under grain crops Invention relates to agriculture. The fertiliser-herbicide mixture for grain crops contains avrotex, water and liquid nitrogen fertilisers KAS-32, with the following ratio of components, wt %: KAS-32 - 30.0-40.0, avrotex - 0.38-0.60, water - the balance up to 100. |
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Strain of Sphingobacterium multivorum RNCIM B-10385 is proposed, used to obtain a bacterial fertiliser for tomatoes and cucumbers. |
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Invention relates to agriculture. The method of producing microbial organomineral fertiliser from humus-containing substances through preliminary mixing of humus-containing substances and a liquid medium, stirring with subsequent extraction of an aqueous extract, wherein the humus-containing substances are mixed with a liquid culturing medium containing living cultures of microorganisms in active form and metabolites thereof, having titre from 1×104 CFU to 1×1010 CFU; the humus-containing substances are mixed with the liquid culture medium in percentage ratio from 0.5% to 99.5% humus-containing substances, the liquid culture medium being the balance; fermentation is carried out while stirring and/or aerating until achieving overall titre of microorganisms from 1×108 to 1×1010 CFU; further, a preservative with bacteriostat properties is added to the obtained mass in concentration from 1 g to 500 g dry preparation or liquid preparation in terms of dry substance per litre of the mass; the obtained mass is filtered, squeezed and the obtained solution is normalised. |
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Method of use of wastes of sugar beet production in beet-seed crop rotation Method involves mixing of sugar beet pulp and a defecate by their distribution on the surface of the soil at a ratio of (1-2):(1-1.5), providing pH of the resulting mixture of 6.8-7.0, in an amount of 12-20 t/ha, then the processing of the mixture by the culture Trichoderma viride, strain 98 or 838, with the norm of the introduction of 5 l/ha and uniform embedding in the soil to a depth of 7-10 cm. |
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Method of processing household wastes Invention relates to biotechnology and specifically to methods of recycling household wastes. The method involves removing inorganic impurities, grinding, mixing wastes with an organic additive containing a culture of microorganisms and composting to obtain organic fertiliser. The organic additive used is compost based on poultry droppings, which is taken in amount of 300-500 kg per ton of wastes, and microbial strains Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp. Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings. |
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Method of producing organic fertiliser from wastewater sludge Invention relates to ecology and can be used in recycling wastewater sludge formed at city aeration stations. The method involves mixing wastewater sludge with storage life of less than 3 years with turf in volume ratio of 0.5:0.5 or 0.6:0.4, respectively. The obtained mixture undergoes aerobic composting in the presence of a biological activator in amount of not less than 15% of the volume of the compost mixture. The biological activator used in the compost mass is inoculating compost based on poultry droppings and microbial strains Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp. Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings. |
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Method of producing biocompost based on cattle dung Invention relates to agriculture and can be used in processing cattle dung. The method involves formation of at least one pile of dung. Inoculating compost is added to the compost mass in amount of 10-15 kg/t. The compost mass is then moistened, biologically heated and periodically mixed. The inoculating compost used is compost based on poultry droppings and a consortium of microbial strains Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp.Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings. |
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Method for biological processing of poultry droppings Invention relates to bioconversion of poultry wastes and can be used in production of ecologically clean effective fertiliser for agricultural crops. The method involves successive layering of droppings and a water-absorbing organic material, forming at least one pile and adding microorganisms in liquid form in form of a suspension, stirring the mixture, biological heating and aerobic fermentation of the mixture. Aerobic fermentation of the mixture is carried out until temperature of the mixture naturally drops to 25-30°C. The microorganisms used are consortium of Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp. Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings. |
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Invention relates to agriculture and can be used in processing organic wastes, particularly manure and turf to obtain organic fertiliser. The method involves layering manure and water-absorbing material - turf, adding compost followed by aerobic composting at 50-60% moisture content of the mixture. The compost used is inoculating compost based on poultry droppings and a consortium of microbial strains Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp. Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings. The inoculating compost is taken in weight ratio to the compost mixture of 1:20. Composting is carried out in piles. |
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Method of producing organic fertiliser from residual products of fish waste processing Invention relates to complex treatment and recovery of fish wastes for production of organic fertilisers. Proposed method comprises loading the mix of organic wastes in reactor, heating it and anaerobic fermentation. Fish press water is used as said mix of organic wastes. Said press water is heated to 80-90° is separated to suppress and extract residual fish oil. Liquid fraction is loaded into reactor for anaerobic fermentation at 50-55°C at constant mixing for 2-3 weeks. Obtained liquid fertiliser is fed into cooling reactor wherein oxygen ingress is either partially limited or completely inhibited. Temperature id reduced for 2 days to 0°C to minus 10°C. Thereafter, fertiliser is packed. |
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Biopaste and method of its production Method includes mechanical grinding of a mixture of pre-moistened biohumus with water in an attritor with the help of metal balls. The mixture amount makes not less than the free volume of space between balls. The biopaste consists of biohumus ground in water and is a homogeneous gel-like mass with high content of useful microorganisms in anabiosis condition and humic substances. Size of biohumus particles is not more than 15 mcm. Biopaste moisture is 70-85%. When interacting with water, biopaste is dissolved without any residue and has extended shelf life. |
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Method of microbiological processing of bird droppings Method includes adding a microbial culture Pseudomonas sp. 114, deposited in VKPM under No. B-5060, into bird droppings, mixing. In 5 days a microbial culture Azotobacter chroococcum B 35 is added, deposited in VKPM under No. B-6010, and again mixed. Titre of added microbial cultures for Pseudomonas sp. makes 114-108 kl/ml, and for Azotobacter chroococcum B 35-108 kl/ml. Volume ratio of added cultures is 2:1 accordingly with 45 ml per 1 kg of bird droppings in case of birds keeping without litters. In case of birds keeping with litters, Pseudomonas sp. 114 and Azotobacter chroococcum B 35, taken as 2:1, are added in amount of 15 ml per 1 kg of droppings. Prior to addition of microbial cultures, each of them is dissolved with water in the ratio of 1:2 accordingly. Microbiological treatment of bird droppings is done for 15 days. |
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Eco-black soil and concentrated soil solution, apparatus and preparation method thereof Invention relates to agriculture and soil science. Eco-black soil consists of soil containing 5-8% humus. The soil structure is formed by fine - 0.1-0.3 mm and coarse - up to 10 mm lumps whose colour varyies from light brown to black and pH 6-8. The eco-black soil is populated with earthworms and microflora typical for virgin black soil and containing cellulose-decomposing fungi, lithotrophic microorganisms, lactic-acid bacteria, nitrogen-fixing bacteria and nitrogen compound decomposers. The concentrated soil solution consists of an aqueous extract of eco-black soil containing dissolved components of humus and microflora, and whose colour varies from light yellow to dark brown and pH 6-8. In the method, a drainage system is formed in insulated vessels and a horizontal layer containing an organomineral mixture is placed over the said drainage system. Eco-black soil containing black soil-forming organisms is put into the horizontal layer in amount of 5-20% of the total volume of the layer. Optimum processing conditions are maintained and at the end of processing, water is sprayed onto the horizontal layer and the filtrate is sorbed. The apparatus contains vessels with a liquid-permeable bottom placed in a room, sprinkling and filtrate collecting devices and devices for feeding and heating air. The vessels are fitted with devices for controlling temperature and moisture. The sprinkling devices are fitted with liquid-spraying apparatus. Inside each vessel from bottom-up there is a drainage layer, a layer of organomineral mixture in whose depression the initial volume of black soil is placed, and the processed layer of organomineral mixture. |
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Bradyrhizobium japonicum BKM B-245 5D nodule bacterial strain used for manufacturing of a bacterial fertiliser stably improving soya seed crop by 3.0 centner/ha on the average. The strain is recovered from soya nodules of the mid-ripening variety October 70, grown up on brown forest soil of Svobodnensky District of the Amur Region. The seed grain is cultivated with using an agarised nutrient medium containing mannitol. Then it is placed in thermostats at temperature 27-28°C for 7 days. The seeds are inoculated on the soya seeding day with using a bacteria-molybdenum compound. The prepared 1.25 % molybdenum solution (ammonium molybdate 25 g/l) 2.5 ml is introduced in test tubes with streaks of the nodule bacterial strain being tested. A streak from a nutrient medium swarm is washed off with the molybdenum solution. By shaking a test tube, a homogeneous bakteria-molybdenum compound (2.5 ml) used for immediate processing of the soya seeds (250 g) is produced. Then, the soya seeds are intimated, dried in the shade and sowed in the prepared soil. |
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Sinorhizobium fredii nodule bacterial strain is recovered from a natural population of nodule bacteria common in soils of a southern zone of the Amur Region and deposited in the Russian National Collection of Microorganisms, No. B-2458D, and also stored in the collection of the State Scientific Institution Russian Soya Research Institution, No. BB-49k. |
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Strain of rhizobia Bradyrhizobium japonicum BM-85k is isolated from natural population of rhizobia, spread in soils of southern zone of Amur region and is deposited in All-Russian Collection of Microorganisms of G.K.Skryabin Institute of Biochemistry and Physiology of microorganisms (IBPM) of Russian Academy of Sciences under registration number "ВКМ В"-2456D. |
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Invention relates to biotechnology, particularly to production of bacterial fertiliser for soya bean. The Sinorhizobium fredii KB-11 legume bacteria strain is extracted from a natural population of legume bacteria common in the south of Amur region and deposited in the All-Russian collection of microorganisms under number B-2457D. |
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Strain of bacteria Bacillus mucilaginosus - Bac 1208, number VKPM V-10209 dated February 16, 2009, increasing capacity of crops. Fertiliser for growth of crops is produced by step fermentation in nutrient medium of specified strain. |
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Strain of bacteria Agrobacterium radiobacter - Ag 1108, deposited on February 16, 2009 in Russian National Collection of Industrial Microorganisms (RNCIM) under number VKPM V-10210. Fertiliser to grow crops is produced by means of step fermentation of specified strain on nutrient medium of Fedorov. |
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Invention relates to biotechnology and specifically to production of biofertiliser and plant protection agents. The method of producing biohumus involves worm composting of a substrate based on organic agricultural wastes using an industrial line of earthworms Red Californian hybrid and adding a culture of microphilic cellulose-lytic fungus Trichoderma viride. The substrate used is a mixture of peat and cattle straw-containing manure taken in weight ratio of 1:4 and moisture of 75-80%, and the culture of microphilic cellulose-lytic fungus Trichoderma viride is added on cooked wheat grain in amount of 2% of the weight of the substrate 2 weeks before releasing the earthworms in amount of 40 mature adults (30-35) g per kg of the substrate. Worm composting is carried out under cradling conditions for 3-4 weeks at temperature +20…+25°C. |
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Biological preparation in form of aqueous suspension for increasing soil fertility Invention relates to biotechnology and agricultural microbiology and specifically to making bacterial preparations based on nitrogen-fixing bacteria used in agricultural engineering for increasing soil fertility and yield. The biological preparation contains Azotobacter vinelandii VKPM V-5933 bacteria strain containing not less than 1×109 cells/ml living bacteria in a culture liquid, sodium chloride, trace elements: zinc, magnesium, manganese, cobalt, molybdenum, boron and water with the following ratio of components, in wt %: bacteria strain in culture liquid - 50-60, trace elements - 0.2-0.3, sodium chloride - 1.0-1.5, water - up to 100%. |
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Method of making bacterial fertiliser based on azotobacter bacteria genus Invention relates to agricultural microbiology and can be used in microbiological industry for making various bacterial fertilisers. The method involves culturing bacteria on a solid Ashby medium and is distinguished by that free aerobic nitrogen fixers are first separated from liquid elective cultures and subsequently cultured into pure cultures on a solid culture medium. The most actively developing strains are separated from the pure cultures and transferred to a liquid culture medium which does not contain nitrogen. A microbiological preparation based on Pseudomonad bacteria genus and a soil filtrate are added to the obtained stable bacterial suspension which stimulates agricultural plant growth. The mixture is thoroughly stirred, the filtered Ashby medium for azotobacter and oligonitrophiles with a solution of Fedorov microelements is then added and culturing in a fermenter is carried out at 22-28°C with the mixer rotating at 180 rpm for 10 days - 4 times a day for two hours. |
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Method for production of biologically active organic fertiliser (baof) Method includes bioconversion of manure with straw for 3 months at the temperature of up to 50°C. Besides bioconversion is carried out with application of strain of bacteria Bac.subtilis TNP-3-DEP as 1 billion of microbial cells per 1 t of organic substance. Process of bioconversion is carried out in aerobic conditions under action of microflora, i.e. with air access in mesophilous mode. |
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Holoplankton fertiliser, method and device for producing said fertiliser Besides fermented phytoplankton, the fertiliser contains fermented zooplankton, which increases biological value of the fertiliser. When making the fertiliser, phytoplankton and zooplankton are fermented together. The device for making the said fertiliser has a pipe-shaped housing, with an inlet window on one side and an outlet window on the other, a working shaft, mounted inside the pipe-shaped housing along its axis, a motor for rotating the working shaft, rotating blades, mounted in series on the shaft with given spacing and directed from the shaft to the inner wall of the pipe-shaped housing, fixed blades, mounted on the pipe-shaped housing in series along its axis with given spacing and directed from the inner wall of the pipe-shaped housing to the shaft. The rotating blades and fixed blades are grouped into steps. |
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Soil MALAKHIT is related to soils based on peat and biocompost obtained by utilisation of solid municipal waste used in 'green' construction for cultivation of flowers and turfgrass (version 1) and coniferous scrub (version 2). Soil includes underdecomposed upper-moor peat with acidity of 2.5 pH or more and biocompost at respective weight ratio of 30:70 (version 1) and 50:50 (version 2), mineral fertilisers represented by nitrogen, phosphor and/or potassium fertilisers depending on volatile nitrogen, phosphor and potassium form content in the biocompost (versions 1 and 2), and dolomite powder (version 1). Biocompost includes aerobic fermentation products of solid municipal waste and OMUT microbe fertiliser (10 wt %) containing association of active microbes Clavibacter michiganese, Bacillus amylognefaciens, Micrococcus various, cultivated in bird droppings. |
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Method of receiving of peat substance for planting stock of forest plants Method of getting peat substance presupposes processing of solid domestic wastes, compost crushing and introduction therein fermentative supplement in the form of active microorganisms association, conducting aerobic fermentation, and receiving biocompost, mixing biocompost with peat and admix of mineral supplements in the form of fertilisers. Fermentative supplement is introduced into the compost in the amount of 10 wt % off the organic fraction of solid domestic waste. Mobile forms of nitrogen, phosphor or potassium in biocompost are detected. Nitrogen and/or phosphorous and/or potassium fertilisers are introduced. Biocompost of 35-50% humidity is mixed with raw highbog peat with acidity of 2.5-3.5 pH at 50:50 weight ratio of biocompost to raw highbog peat. |
Another patent 2550900.