Strain of nodule bacteria bradyrhizobium japonicum 859 for production of fertiliser for soya

FIELD: biotechnologies.

SUBSTANCE: strain of nodule bacteria Bradyrhizobium japonicum 859 (VNIISHM No.24117) is proposed, used as a facility to produce a fertiliser for soya. When soya seeds are treated with the fertiliser produced on the basis of the specified strain Bradyrhizobium japonicum, the index of germination increases by more than 8%, and crop capacity increases by 17-30%.

EFFECT: higher germination of plants, improved crop capacity.

4 tbl

 

The invention relates to biotechnology and agriculture and for the new strain of nodule bacteria Bradyrhizobium japonicum 859 for obtaining microbiological fertilizer, increasing the yield of soybean.

At the present time to increase the yield of legumes using bacterial fertilizers, manufactured on the basis of nitrogen-fixing nodule bacteria.

So, from the description to the Author's certificate of the USSR No. 491695 known strain of Rhizobium japonicum 646, which is used for the manufacture of nitragin. Its disadvantage is the relatively low nitrogen-fixing activity and competitive ability.

From the description to the Author's certificate of the USSR No. 640989 (published 08.01.1979) known strain of nodule bacteria of soybean Rhizobium japonicum a, which is highly symbiotic fixer. Its disadvantage is the sensitivity to chemical seed dressing, so processing of seeds of legumes is required immediately before sowing, not combining it with chemical etching.

From the description to the Author's certificate of the USSR No. 1034358 (priority from 04.01.1982, published 07.04.1984,) also known strain of nodule bacteria of soybean Rhizobium japonicum 634 B, which is used in the industrial production of bacterial drug nitragin for soy. While specified Stamm B actively fixes atmospheric nitrogen in symbiosis with soybean, promotes active accumulation of the protein in plants. Its disadvantage is the relatively low efficiency.

A known strain of bacteria Rhizobium japonicum 24108 (t), described in the materials to Author's certificate No. 939547 (priority from 31.07.1990 published 30.06.1993 g), which allows to obtain an increased yield of green mass and soybean compared to other well-known at the priority date of the same strains. Its disadvantage is the low competitiveness of local strains of root nodule bacteria of soybean.

There are a number of strains of root nodule bacteria for the industrial production of bacterial preparations for soy described in Ukrainian patents, for example:

- Bradyrhizobium japonicum M-8 (declarative patent (UA) for the invention 39545 And published 15.06.2001,);

- Bradyrhizobium japonicum 10 KN (declarative patent (UA) for the invention 69993 And published 15.09.2004,);

- Bradyrhizobium japonicum kzt66 (patent (UA) for the invention 83298 C2, published 25.06.2008,);

- Bradyrhizobium japonicum 36 (patent (UA) for a utility model 36700 U posted, 10.11.2008);

- Bradyrhizobium japonicum 46 (patent (UA) for the invention 85943 C2, published 10.03.2009,).

Their disadvantage is that these strains were selected and tested on soybean varieties cultivated in Ukraine, in relation to local soil and climatic conditions. Thus, there are no Dan is haunted by the effectiveness of these strains in soil and climatic conditions of the main regions of cultivation of soybean in Russia: Krasnodar, Stavropol territories, Rostov region, in the far East, Siberia.

Also known microbiological preparation of Agrostim B containing a consortium of strains of Pseudomonas fluorescens and Azotobacter spp. (see, for example, the article "increasing the yield of leguminous crops through the use of the drug Agrostim B", the authors Kirsanov E.V., Daroga C.V. / FGOU VPO eagle RAY, Musatova N.N., Tsukanov SR, Borzenkova GA/ SSI VNIIZBK/, published in the journal Bulletin of the eagle RAY # 4, 2008 UDC 635. 656: 633.353: 635.655). Such medication can be used for pre-sowing seed treatment and spraying the soil and vegetative plants. He has growth-stimulating and overwhelming harmful microflora action. The disadvantage is that when processing the specified drug on the roots of plants not there occurs the formation of nodules, fixing necessary for plant nutrition atmospheric nitrogen, which does not allow to greatly improve the yield of soybean and leads to the necessity to enter into the soil addition of nitrogenous fertilizers.

It is also known microbiological fertilizer for soy Risotorphine from the strain of nodule bacteria of soybean Rhizobium japonicum 629and(prototype), as described in the materials for the Author's certificate No. 939547 (priority from 05.01.1981, published 30.06.1982 g), which allows to obtain the soybean crop with high retained the eat protein in the grain. The specified strain 629and(87) is suitable for the industrial production of drugs nodule bacteria (Isotropin). The disadvantage is the relatively low efficiency at the primary production soybean varieties grown on soils of the Far East.

The task of the invention is the identification of a new strain of bacteria with high nitrogen-fixing activity, suitable for use in agriculture to increase soybean yields.

This problem is solved due to the fact that fertilizer under the soybeans used a strain of nodule bacteria Bradyrhizobium japonicum 859.

The bacterial strain was isolated from nodules growing on powerful Chernozem Gulkevichsky district, Krasnodar territory soy and was originally named as the strain of Bradyrhizobium japonicum M-8, which was deposited in the collection of the GSI ARRIAM RAAS 05.12.2008, under registration number 24117 in the group of root nodule bacteria. Then, as a result of a patent search revealed that in Ukraine the other strain was named Bradyrhizobium japonicum M-8 Kircyner (declarative patent (UA) for the invention 39545 And published 15.06.2001, then, to avoid problems with infringement of intellectual property, selected by the Applicant strain was renamed 11.02.2010, in a strain of Bradyrhizobium japonicum 859.

The strain of Bradyrhizobium japonicum 859 characterized SL is blowing morpho-cultural, physiological and biochemical characteristics.

The cells have the shape of a regular sticks with rounded ends and a polar arrangement of flagella. The size of cells(0,6-0,8)×(1,4-1,9) um.

The strain of Bradyrhizobium japonicum 859 does not form spores, negatively stained by gram stain. Growth in liquid and semi-solid nutrient medium aerobic respiratory metabolism. On Mannino-yeast agar forms mucoid, convex, round, opaque colonies with smooth edges, with a diameter of 2-5 mm, the Optimum temperature for growth of 28°C. At temperatures less than 20°C over 35°C, the growth slowed down. The optimum value of pH 6.8, growth also occurs with a pH from 5.0 to 8.5. Aerobe, demanding to oxygen.

The strain of Bradyrhizobium japonicum 859 not hydrolyzes casein, gelatine. With the growth of the strain on litmus milk is observed mild, kislotoobrazovanie with the changing tone of the environment when compared with control. As the only carbon source strain capable of use with the formation of acid, the arabinose, xylose, glucose, galactose and fructose. The strain is not able to use mannitol, sucrose and lactose.

For storage and cultivation of strains of Bradyrhizobium japonicum 859 in laboratory conditions using Mannino-yeast agar (MDA) at pH=6,8±0,2 following composition, g/l:

Mannitol- 10,0
Yeast extract- 0,5
K2HPO4- 0,5
MgSO4×7H2O- 0,2
NaCl- a 0.1
Agarto 15.0

As you know, for the development of plants need nitrogen. Nitrogen fixation is the biological absorption of molecules of nitrogen-fixing bacteria with the formation of nitrogen compounds available for use by other organisms; is one of the most important processes of the nitrogen cycle in nature and an indicator of soil fertility. Nitrogen fixation is carried out as free-living nitrogen-fixing bacteria, Azotobacter, cyanobacteria, Azospirillum and other (nesimpatiska nitrogen fixation)and symbiotic nitrogen fixing bacteria living in symbiosis with higher plants (e.g., rhizobia). In this regard, the nitrogen-fixing activity of the strain is an important characteristic affecting the stimulation of plant growth and yield.

Identified and experimentally established that bacteria declared strain of Bradyrhizobium japonicum 859 form on the roots of plants nodules, have good fixing properties and the good affect germinability of soybean, its growth and yield. The concentration of the bacteria strain for its effective use in a variety of biological products shall be not less than 108CFU/ml were determined Experimentally that the optimum concentration of bacteria in range (108-109)CFU/ml, as the use of strain at a concentration of less than 108CFU /ml leads to a reduction of this effect, and the increase in concentrations in excess of 109CFU /ml did not lead to its increase.

The ability of bacteria declared strain of Bradyrhizobium japonicum 859 to form on the roots of plants nodules confirmed by vegetation experiments conducted at the experimental base of the GSI ARRIAM. First prepared the soil by mixing 80% of the soil from the experimental field (luvisol) with 20% peat soil of the brand "Terravita". Then, the soil was put on 5 kilos in pots with a volume of 3 l and watered with tap water 700 ml per vessel. After that laid the seeds for germination: first, the seeds were surface sterilized with 70% ethanol for 2 min, then washed them with sterile water three times for 2 min, after which sterile seeds were germinated in Petri dishes in physiological solution (0.85 per cent) NaCl) at a temperature of 28°C for 3 days, and the seedlings were soaked in a bacterial suspension of Bradyrhizobium japonicum 629and 7CFU/ml for 15 min and were planted in the soil. At the end of the experiment the plants were dug out with the roots, the roots were washed, dried and weighed separately aboveground and underground parts of plants, counted the number of nodules. The results described vegetation experiments with soybean are presented in table 1.

Table 1.
The number of nodules of soybean and weight of plants in a pot experiment with soy
Version of experienceThe weight of the plant, gThe number of nodules
Control - no processing2,550
Treatment bacterial strain of Bradyrhizobium japonicum 629anda 3.8718,6
Treatment bacterial strain of Bradyrhizobium japonicum 8594,5527,3

From the data presented in table 1, it follows that during the processing of soybean seeds with bacteria declared strain of Bradyrhizobium japonicum 859 compared with control plants soybeans have more weight on their root nodules, and in larger quantities than is the processing of a bacterial strain of the prototype of Bradyrhizobium japonicum 629 and.

The efficiency of the bacterial strain of Bradyrhizobium japonicum 859 tested in pot and field experiments with microbial fertilizers on the basis that the applicant named Bioveris. When studying the effectiveness of the bacterial strain of Bradyrhizobium japonicum 859, it was found that pre-treatment of soybean seeds fertilizer of Bioveris affect their germination and yield of soybean. This fertilizer just prior to planting soybeans were processed at the rate of 0.3-0.7 liters of Bioveris on a hectare norm of soybean seeds, which depends on the soybean varieties and ranges from 70 to 120 kg/ha

Table 2 presents the results of experiments on determination of the field of seed germination and yield of soybean varieties Lanceolate, depending on the processing microbiological fertilizers.

Table 2.
The effect of microbiological fertilizers, including Bioveris on seed germinability and soybean yields
Version of experienceField germination, %Harvest
ness, t/ha
Weight of 1000 seeds, gThe protein content in the seeds, %
1. Control (without treatment) 742,0711436,6
2. Microbiological preparation of Agrostim B (Pseudomonas fluorescens+Azotobacter spp.). Seed treatment + spray of seedlings at day 7 after seeding792,4711737,0
3. Risotorphine (Bradyrhizobium japonicum 629and). Seed treatment772,4211537,2
4. Bioveris (Bradyrhizobium japonicum 859). Seed treatment802,4811837,5

From the data presented in table 2, it follows that during the processing of soybean seeds fertilizer of Bioveris the rate of germination was increased compared with control by 8.1%as compared with the fertilizer Risotorphine - 3.9%.

Through the use of fertilizers of Bioveris the excess length of seedlings compared with the control reached 14%, rootlets, up 18%, as compared to fertilizer Risotorphine to 4% and 7%, respectively.

Presented in table 2, the data suggest that the processing of soybean seeds fertilizer of Bioveris its productivity is increased compared with control by 0.41 t/ha or 19.8% as compared with the fertilizer Risotorphine 0.06 t/ha or 2.5%.

The increase in soybean yields due to application of fertilizer Bioveris mainly due to increased plant productivity, i.e. by increasing compared with the control quantity of beans to 21 percent, the number of kernels Bob - up to 12%, the number of seeds plant - up to 24%, weight of seeds plant - up to 25%.

In addition, during the processing of soybean seeds fertilizer of Bioveris noted the improved quality of soybean seeds in the form of increased content of protein on average by 0.9% compared with the control, 0.5% compared with the drug Agrostim B and 0.3% compared with the fertilizer Risotorphine.

At the same time to achieve the same agronomic indicators (field germination and yield of soybean with drug Agrostim B it took to process them not only seeds, but also to spray the seedlings on day 7 after seeding, which confirms the greater fertilizer efficiency of Bioveris from the strain of nodule bacteria Bradyrhizobium japonicum 859 compared with microbiological drug Agrostim B.

Thus, in table 2 data show greater fertilizer efficiency of Bioveris obtained on the basis of the claimed strain of nodule bacteria Bradyrhizobium japonicum 859, not only in comparison with the drug Agrostim B containing a consortium of strains Pseudoonas fluorescens and Azotobacter spp., but compared with the fertilizer Risotorphine obtained on the basis of strain-prototype nodule bacteria Bradyrhizobium japonicum 629and.

That is, from table 2 it follows that the claimed strain of nodule bacteria Bradyrhizobium japonicum 859, characterized by a higher nitrogen-fixing activity in comparison with the known strain of nodule bacteria Bradyrhizobium japonicum 629andthat has a positive effect on the stimulation of plant growth and yield of soybean, as well as on improving the quality of soybean seeds in the form of increased content of protein.

All of the above indicates the possibility of using strains of nodule bacteria Bradyrhizobium japonicum 859 for manufacturing products - fertilizers for soybean.

The following is an example of getting the liquid form of fertilizers on the basis of the proposed strain of nodule bacteria Bradyrhizobium japonicum 859.

Uterine culture

To obtain uterine culture of the strain of nodule bacteria Bradyrhizobium japonicum 859 used liquid Mannino-yeast medium, which was poured into 100 to 150 ml Catalogne flask with a volume of 750 ml Autoclaving was carried out at a temperature of at least 121°C for 20-30 minutes. After that, the medium in each flask was seeded flush with one test tube with a beveled MDA with a pure culture of bacteria from the refrigerator, after which the flask was placed on a shaker (220±10 rpm) for 72±3 hours at a temperature of 2830°C. Thus in the flasks was obtained uterine culture with a titer of bacteria (3-5)·109CFU/ml, which was stored in the refrigerator for subsequent inoculation of flasks or fermentors.

The working culture

For the industrial cultivation of strains of Bradyrhizobium japonicum 859 used nutrient medium at pH=6,8±0,2 following composition (with a possible deviation of ±5% from the specified amount, g/l: corn extract - 7,0; molasses - 5,0; (NH)2SO4to 1.0; K2HPO4- 0,35; KH2PO4- 0,35; MgSO4×7H2O - 0,2; caso3to 1.0, which sterility was subjected to autoclaving at a temperature of at least 131°C for at least 60 minutes. The sterile nutrient medium was tested by seeding sterile samples taken from the fermenter to the standard of the finished medium: nutrient agar (manufacturer LLC Biocampus-With", Russia) or a triptych soy agar (TSA, the manufacturer, the company Sigma, USA) and judged it by the absence of bacterial growth within 48 hours at a temperature of 28±1°C. thus Prepared sterile nutrient medium in the fermenter was seeded uterine culture of a strain of Bradyrhizobium japonicum 859 with a titer of bacteria (3-5)×109CFU/ml, and the volume of inoculum was 5-10% of the volume of the seed culture medium and were grown for 72±3 hours at a temperature of 28±1°C and p is odowka sterile air at the rate of: volume of air per minute per volume of culture medium (with a possible deviation from this ratio ±5%). The sterility of the air provided by blowing through installed at the inlet of the fermenter bacterial filter, for example, the brand Sartorius Stedim Biotech producer JSC "SARTOGOSM". Thus, before implementing cultivation of strains of Bradyrhizobium japonicum 859 have been checking the degree of sterility of the supplied air by blowing through the bacterial filter at the inlet of the fermenter within 24 hours with subsequent seeding sterile taken from the fermenter culture medium on the standard of the finished medium: nutrient agar (manufacturer LLC Biocampus-With", Russia) or a triptych soy agar (TSA, the manufacturer, the company Sigma, USA) and judged it by the absence of bacterial growth within 48 hours at a temperature of 28±1°C.

The obtained culture liquid strain of Bradyrhizobium japonicum has 859 microscopically and were sown on Mannino-yeast agar (MDA) for counting the number of nodule bacteria and the triptych-soy agar (TSA) for the calculation of the number of foreign microorganisms.

So get concentrate bacterial suspension fertilizers based on the strain of bacteria Bradyrhizobium japonicum 859 with a titer of not less than 109CFU/ml

The liquid form of fertilizer Bioveris from the strain of nodule bacteria Bradyrhizobium japonicum 859

Microbiological fertilizer in liquid form from the strain of nodule bacteria Bradyrhizobium japonicum 859 which was alocale by diluting the concentrate bacterial suspension with water, and used a sterile, distilled water, obtained, for example, by setting the fine chemical, mechanical cleaning and disinfection of water Geyser (producer JSC "Eco Geyser", Russia). The acceptable level of sterility of the water was determined by plating on nutrient agar and free of bacterial growth within 48 hours at a temperature of 28±1°C.

Receiving fertilizer in sterile water were added the following ingredients per 1 l of water (with the possible ingredients for a deviation of ±5% from the specified amount, g/l:

concentrate bacterial suspension-100,0
K2HPO4(potassium hydrosulfate)- 1,5
KH2PO4(hydroalcoholic potassium)- 1,5
MgSO4·7H2O (heptahydrate sulphate of potash)- 1,5
a. (humic fertilizers)- 2,0

Then determined and brought the pH of the resulting fertilizer to 6.8 to 7.2.

The liquid fertilizer was kept for 3-5 days at a temperature of 25-28°C to obtain the title tank is ERI Bradyrhizobium japonicum 859 in the resulting fertilizer is not less than 10 8CFU/ml

After this liquid microbiological fertilizer based on the strain of bacteria Bradyrhizobium japonicum 859 that the applicant named Bioveris was ready for processing agricultural crops, namely soybeans.

Strict control and appropriate management of the process of preparing liquid formulations require high quality fertilizer of Bioveris and its corresponding activity during the processing of soybean seeds.

Checking the efficiency of fertilizer Bioveris from the strain of nodule bacteria Bradyrhizobium japonicum 859

The efficiency of microbiological fertilizer Bioveris derived from strains of nodule bacteria Bradyrhizobium japonicum 859, was tested on the production experiments in Ulyanovsk and Amur regions.

Just prior to planting one part of soybean seeds did not treated (control), another part of the seed was treated with liquid fertilizer Risotorphine from the strain of nodule bacteria Bradyrhizobium japonicum 629andand the last part was treated with fertilizer, obtained as described above, the rate of 0.5 liter of Bioveris on a hectare norm of soybean seeds.

The results of the experiments are shown in Tables 3 and 4.

From the data presented in tables 3 and 4, it is seen that the application of liquid fertilizer of Bioveris received n the basis of nodule bacteria strain of Bradyrhizobium japonicum 859, increased soybean crop in comparison with the control (without fertilization) on average 17-30%, which is more efficient than the use of fertilizers Risotorphine-based strain-prototype nodule bacteria Bradyrhizobium japonicum 629and.

From table 4 it follows that the application of the far East - in areas of the Amur region is declared the strain of nodule bacteria Bradyrhizobium japonicum 859 most effective in comparison with the strain of nodule bacteria Bradyrhizobium japonicum 629andthe prototype.

The strain of bacteria Bradyrhizobium japonicum 859 deposited in the collection of wildebeest WNISM RAAS 05.12.2008, under registration number 24117 group nodule bacteria as a means to obtain fertilizer for soy.



 

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21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: medicine.

SUBSTANCE: present invention refers to biotechnology and medicine. There are presented versions (aCt1 and aCt2) of one-domain antibodies specifically binding the Chlamydia trachomatis antigen. There are described versions of the method of inhibiting an infection caused by Chlamydia wherein the method involves the preparation of elementary bodies C.trachomatis by a therapeutically effective amount of the nanoantibody aCt1 or aCt2 before being attached to infected target cells.

EFFECT: use of the invention provides the antibodies to detect and block the infections Chlamydia trachomatis that can find application in medicine.

6 cl, 4 dwg, 5 ex

FIELD: biotechnology.

SUBSTANCE: basic fermentation medium is prepared on the basis of fermentative lysate of starch in an amount of 8-15% for glucose, and additional fermentation medium containing the components of basic fermentation medium, 2-3.5 times concentrated. They are sterilised. Biosynthesis of L-lysine is carried out using strain-producer Corynebacterium glutamicum of All-Russia classification of microorganisms Ac-2577D (BIGOR 55). The fermentor is loaded with the basic nutrient medium in amount of 1/3 of its volume. Then inoculation of the basic fermentation nutrient medium is carried out with 10-20% inoculum grown in two stages. Biosynthesis process is carried out with continuous feeding starting from 8-16 hours of cultivation. At that the inhibitory concentration of reducing substances in the culture fluid of 4-8% is maintained. After filling the device to the maximum volume after 60-66 hours of cultivation and then every 6-12 hours for the rest part of biosynthesis process a part of the culture fluid in amount of 10-15% of the maximum volume is pumped into the device for final fermentation. In this device, the biosynthesis process is continued for 6-12 hours under the same conditions as in the main device but without feeding the additional nutrient medium. Then L-lysine is isolated.

EFFECT: invention enables to carry out the biosynthesis process for a long time without reducing the rate of synthesis of L-lysine, and provides obtaining of the culture fluid with high concentration of L-lysine prior to the isolation.

4 ex

FIELD: biotechnology.

SUBSTANCE: isolated recombinant adenosine deaminase is described, which comprises polypeptide SEQ ID NO: 1 or a version polypeptide SEQ ID NO: 1 of isolated recombinant adenosine deaminase, where the version polypeptide SEQ ID NO: 1 comprises one or more amino acid substitutions selected from the group consisting of: Gin instead Lysl98; Ala instead Thr245; and Arg instead of Gly351, and DNA encoding it. The conjugate of polyalkylene oxide with the said adenosine deaminase for treatment of adenosine deaminase-mediated diseases is presented where adenosine deaminase comprises from 11 to 17 chains of polyalkylene oxide with a molecular weight of 5 kDa for ADA protein. The methods of purification of the recombinant adenosine deaminase are proposed, including protein purification using ion exchange chromatography, or protein purification using hydrophobic interaction chromatography. Also the preparations of recombinant adenosine deaminase produced by these methods are provided.

EFFECT: invention enables to obtain recombinant adenosine deaminase, having increased stability.

14 cl, 1 tbl, 10 ex

FIELD: biotechnology.

SUBSTANCE: culture fluid obtained after growth of the mixture of strains Lactobacillus acidophilus NKI, 100ash, K3"Ш"24, is frozen, the fat layer is removed, the defatted part is thawed and the cells are separated by centrifugation. The obtained supernatant is sterilised by membrane vacuum filtration, is concentrated with membrane ultrafiltration in the process of centrifugation, the concentrate of supernatant is treated with the components more than 30 KDa with fivefold excess of ice acetone. The resulting precipitate is diluted with 20 mM NaCl at a ratio of 2:1 by volume and is mixed with 10% Tween-80 to a final concentration of 1% Tween. Purification and isolation of the target product is carried by the method of horizontal isoelectrophoresis in a plate of 5% polyacrylamide gel with 7-8 M urea and 5% sucrose in gradient pH 4-8 at a temperature 8-12°C for 8 hours When isolation of the target product after electrophoresis the gel stripes are cut on isopoints, frozen at a temperature (-20°C) for 2 hours, thawed and dispersed, the solid parts are separated by centrifugation, concentrated by centrifugation at 5500 rev/min for 60 min at room temperature, low molecular weight impurities are removed, and the concentrate is mixed with phosphate buffer in 100-fold volume The gel stripes are cut on isopoints with pl (4.0-4.3) with the isolation of acid lectins and pl (7.6-8.0) with the isolation of alkaline pectins.

EFFECT: obtaining of lectins having anti-candidal activity is provided

5 cl, 1 tbl

FIELD: biotechnology.

SUBSTANCE: invention relates to a strain of the bacteria Lactobacillus plantarum Inducia DSM 21379 used as a probiotic. The strain enhances the natural protective potential of organism, produces polyamines from ornithine and glutamate, nitric monoxide (NO) from arginine, possesses antioxidative activity and enhances the intestinal barrier function, stimulating cellular immunity of the intestinal mucosa and blood, and stimulates adaptively the proinflammatory cytokines. The strain in an amount providing an effective daily dose of from 108 to 109 CFU/ml or CFU/g is used for preparing a composition for use as an antioxidant probiotic.

EFFECT: increased cellular immunity.

7 cl, 8 dwg, 14 tbl, 6 ex

FIELD: biotechnology.

SUBSTANCE: strain Bacillus licheniformis CL-13 which has high histidine decarboxylase activity is deposited in the Russian National Collection of Industrial Microorganisms (RNCIM) under the registration number of RNCIM B-11185 and can be used in histidine decarboxylase of enzymatic preparation.

EFFECT: invention enables to obtain a strain of histidine decarboxylase activity.

1 ex

FIELD: chemistry.

SUBSTANCE: method of producing copolymer of 3-hydroxybutyric acid and 3-hydroxyvaleric acid involves culturing a Ralstonia eutropha All-Russian collection of industrial microorganisms V-5786 producer strain in aeration conditions and mixing on a liquid salt medium which contains a hydrogen- and carbon-containing growing substrate with addition of a salt of valeric acid. The strain is cultured in two steps while continuously feeding a nitrogen source for 24-36 hours at the first step and without nitrogen at the second step. The growing substrate used is a gaseous mixture of H2, CO2, CO and O2, wherein O2 is fed into the culture in a separate stream while continuously feeding potassium valerate into the culture.

EFFECT: high total output of the copolymer.

1 tbl, 3 ex

FIELD: biotechnologies.

SUBSTANCE: strain of bacterial Pseudomonas panipatensis VKPM V-10593 is proposed, which is capable of quick recycling of oil and oil products, in particular, diesel fuel. Strain may be used to clean soil and water reservoirs contaminated with oil and oil products, in a wide range of temperatures from +8 to +30°C.

EFFECT: improved properties of a strain.

2 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry. Disclosed is a method of delaying plant development processes, associated with ethylene biosynthesis involving exposing a plant or plant part to one or more bacteria which produce one or more enzymes selected from a group consisting of nitrile hydratase, amidase, asparaginase, and mixtures thereof and said one or more bacteria are selected from a group consisting of Rhodococcus, Brevibacterium ketoglutamicum, and mixtures thereof, and wherein said one or more bacteria act on the plant or plant part in an amount sufficient to delay the plant development process. Disclosed is an apparatus for delaying the plant development process associated with ethylene biosynthesis having several layers. At least one of the layers contains a catalyst which contains one or more bacteria selected from a group consisting of Rhodococcus, Pseudomonas chloroaphis, Brevibacterium ketoglutamicum and mixtures thereof, wherein the one or more bacteria produce one or more enzymes selected from a group consisting of nitrile hydratase, amidase, asparaginase and mixtures thereof.

EFFECT: invention increases efficiency of delaying plant development process associated with ethylene biosynthesis.

59 cl, 4 dwg, 9 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry and biotechnology and can be used in production of probiotic bacterial preparations, biologically active food additives, fermented milk and non-fermented food products. The Lactobacillus plantarum VKPM V-11154 strain has high antagonistic activity with respect to pathogens and opportunistic pathogens, including Candida yeast fungi. The strain is phenotypically apathogenic, has average adhesion and self-aggregation properties, high surface hydrophobicity, average coaggregation; hydrochloric acid has minimum inhibiting concentration of 1.25%; the bile stimulates propagation and growth of the culture.

EFFECT: invention enables faster accumulation of biomass with high concentration of lactic bacteria on artificial nutrient media.

2 dwg, 5 tbl

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture. The method of cultivation of soyabean using polycomponent fertiliser "Bioplant Flora", including presowing treatment of seeds with rizotorfin. The presowing treatment with rizotorfin is performed in the mixture with polycomponent fertiliser "Bioplant Flora" at a dose of 1 l/t. At that foliar fertilising of plants is carried out with the polycomponent fertiliser "Bioplant Flora" in the phase of 4-5 leaves and end of bean-formation at a dose of 0.25-1 l/ha.

EFFECT: method enables to improve the yield and quality of soyabean by increasing the amount of nitrogen-fixing nodules on soybean root system and improve the nutrient regime of plants, also it is more efficient to use the elements of mineral nutrition of fertiliser, thus reducing human-induced impact on the environment.

4 tbl, 1 ex

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