Method for early prediction of risk of developing peripheral vitreochorioretinal dystrophies on paired eye after regmatogenous retinal detachment surgeries |
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IPC classes for russian patent Method for early prediction of risk of developing peripheral vitreochorioretinal dystrophies on paired eye after regmatogenous retinal detachment surgeries (RU 2540505):
 Method of producing 3-phenylimino-3h-phenothiazine or 3-phenylimino-3h-phenoxazine mediator / 2524660
Invention relates to a method of forming a 3-phenylimino-3H-phenothiazine mediator or a 3-phenylimino-3H-phenoxazine mediator, which includes providing a first reactant including phenothiazine or phenoxazine, providing a first solvent, providing a second reactant and providing a second solvent. The first reactant, first solvent, second reactant and second solvent are combined to form a reactant solution. Sodium persulphate is added to the reactant solution to link the first and second reactants resulting in a reaction solution containing the 3-phenylimino-3H-phenothiazine or the 3-phenylimino-3H-phenoxazine mediator. Further treatment of the reaction solution is carried out to remove the first solvent and second solvent in order to separate the mediators. The invention also relates to products obtained using said method and use thereof in an electrochemical test sensor.
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Set of inventions relates to measurement of volume or concentration of biological matters. Proposed measuring device comprise casing and at least one display integrated therein. Note here that said casing comprises compartment for fitting the cartridge in said casing to fit specimen to be measured. Note also that said compartment has hole made at casing front. Note that casing first part for cartridge to be fitted therein extends at the angle to casing second part. Besides, this invention covers the method of measurement with the use of described device in analysis of biological fluids.
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Invention relates to analytic devices, and namely to a strip for analytic testing on an electrochemical base that includes an electrically insulating substrate, a fermentative reactant layer located above the substrate, an upper layer located above the fermentative reactant layer; with that, the upper layer has the first part and the second non-transparent part; with that, the first part is transparent or non-transparent, and a sample receiving chamber located inside an analytic testing strip; besides, the sample receiving chamber has an operating part and a non-operating part, in which the first part and the second non-transparent part of the upper layer have the possibility of user's viewing of the operating part of the sample receiving chamber through the first part of the upper layer and impossibility of viewing the non-operating part of the sample receiving chamber due to the second non-transparent part of the upper layer; with that, the operating part of the sample receiving chamber is approximately 86% of the sample receiving chamber.
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What is described is a method for bile lithogenicity test consisting in measuring its physical-chemical properties, with preparing bile dilution series of different concentrations: 1:1 and 1:2, passing a current of 0.01, 0.04, 0.08 and 0.1 mA through a flow electrode of a Joule metre, determining current work versus time for each sample, assessing the dynamics of current-voltage characteristics: if a current work is from 0.6 mcJ and more (in average 1.0±0.29 mcJ), the bile lithogenicity is considered to be high; if a current work is within the range of 0.07 to 0.6 mcJ (in average 0.35±0.18 mcJ), the bile lithogenicity is low.
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 Differential diagnostic technique for haemorrhagic and ischemic strokes / 2503002
What is presented is differential diagnostic technique for acute haemorrhagic and ischemic strokes. The amount of low-molecular DNA fraction is specified by comparing 24-hour electrophoretogram from patient's blood plasma to the standard known concentrations. If the amount of low-molecular DNA 3 hours after the onset of acute stroke is 80-87 ng/ml, haemorrhage stroke is diagnosed. If the amount of low-molecular DNA 24 hours after the onset of acute stroke is 80-87 ng/ml, ischemic stroke is diagnosed.
Instant diagnostic technique for enterovirus antigens in cerebrospinal fluid / 2486520
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In the method of choosing a surgical approach to sialolithiasis, while localising a concretion in the parotid or submaxillary duct, the Lithos System diagnostic kit is presented to be used to examine a sample of non-stimulated saliva from the gland duct. In the presence of the radially directed leave-shaped structures at the facie edge, a concretion is suggested to be removed with the salivary gland, while in the absence of the radially directed leave-shaped structures at the facie edge, the concretion is recommended to be removed from the duct with the salivary gland preserved.
 Low sample volume electrochemical test sensor / 2475731
Electrochemical test sensor for determining concentration of analyte in a liquid sample has: a base with a counter electrode and a working electrode which is electrically connected to an electric current detector; a reagent layer containing an enzyme which reacts with the analyte; a cover which is linked to the base and forms a capillary space with an opening for feeding the liquid sample. The sensor has a dielectric layer which forms a dielectric window, the size of the working electrode being restricted in one direction by the dielectric window, the size of the counter electrode (42) being restricted in one direction by at least the dielectric window (50b) and the capillary space (58) and the size of the dielectric window being different from the size of the capillary space. The group of inventions also relates to a version of the electrochemical test sensor, wherein the size of the counter electrode (42) is restricted at least in part by the end of the capillary space (58c) and stretches along the entire width of the capillary space (58), wherein at least part of the width of the counter electrode is larger than the width of the working electrode.
Gas chromatography method for assessment of oropharyngeal disbiotic conditions in children / 2473902
For the purpose of gas chromatography analysis of oral fluid in children, a sample to be examined is additionally acidified by trifluoromethyl sulphonic acid or fluorosulphonic acid, or 40% aqueous perchloric acid to pH 1.9-2.1. The values derived by this method can be used in monitoring of the effectiveness of therapeutic measures, studying of an effect of new pharmaceutical preparations and prebiotics on the normal microflora.
 Identification method of blue pus bacillus pseudomonas aeruginosa / 2540501
Identification method of blue pus bacillus Pseudomonas aeruginosa involves inoculation of the test material on a hard substrate, incubation of the inoculum under anaerobic conditions at the temperature of 37°C during 16-18 hours. The obtained bacterial mass in the amount of one bacteriological loop is placed in 300 mcl of a physiological solution and warmed-up at the temperature of 98-99°C during 20-30 minutes and centrifuged at 12000 revolutions per minute during 30 seconds. To a supernatant there added is colouring material for electrophoretic detection in the quantity of 0.5 mcl and 20 mcl is added to a well with a size of 4×1 mm 1.2% agarose gel on TAE-buffer with 10 mcl of 1% ethidium bromide. Besides, the amount of 3 mcl of the solution of standard DNA-marker 1 kb containing DNA fragments in the range of 250-10000 bp is added to the test well. Horizontal electrophoresis is performed during 15-20 minutes, and at detection on the obtained electrophoregramme of the test bacterial mass of three strips radiating in ultraviolet light, one of which corresponds to fragments of standard DNA-marker with the size of 10000 bp, the second one corresponds to fragments of standard DNA-marker with the size of 6000-8000 bp and the third strip at the end of the track in the form of a whisk, which corresponds to fragments of standard DNA-marker with the size of less than 750 bp, there identified is Pseudomonas aeruginosa in the test bacterial mass.
 Method for prediction of anembryonic pregnancy / 2540499
Method involves dropping a supernatant of uterine fluid aspirate or menstrual fluid in an amount of 0.2 ml on a slide surface, drying at room temperature for one day, and microscoping the prepared samples (phases). The presence of a complex of morphological elements, including toxic plaques, wedge and disk-shaped structures enables predicting a high risk of anembryonic pregnancy.
Early diagnostic technique for chronic prostatitis by detecting regional microvascular endothelium dysfunction / 2540495
Invention refers to medicine, namely to a diagnostic technique for chronic prostatitis by detecting a regional microvascular endothelium dysfunction. Substance of the invention consists in conducting an ionophoretic assay with underlying laser doppler flowmetry, measuring a capillary blood flow reserve with an acetyl choline and sodium nitroprusside ionophoresis on the belly skin above a pubis in a projection point of the prostate, calculating an endothelial function coefficient (EFC), and if the derived EFC is less than 1.0, chronic prostatitis is diagnosed.
 Method of prediction of meat productivity of broiler chickens / 2540435
Method enables to predict the level of body weight gain of broiler chickens on biochemical indicators of blood. This goal is achieved by calculating the lipoprotein index (LPI) in standard units by the formula:
Method for assessing disturbed haemoglobin oxygenation with reduced microviscosity of peripheral erythrocyte membrane of pregnant women in third trimester of gestation and aggravated cytomegalovirus infection / 2540425
Anti-cytomegalovirus antibody titre, microviscosity of erythrocyte membranes are measured in the peripheral blood by eximerisation of a lipid bilayer and a protein-lipid layer; the number of degenerative forms of erythrocytes, target-like erythrocytes and echinocytes, oxyhaemoglobin content are also measured. If the cytomegalovirus antibody titre is 1:1600, decreased eximerisation of the lipid bilayer to 0.59±0.006 FE/FM, of the protein-lipid layer to 0.70±0.004 FE/FM, increased number of the degenerative forms of erythrocytes to 10.5±0.3%, of the echinocytes to 8.0±0.5%, of the target-like erythrocytes to 11.5±0.9%, reduced oxyhaemoglobin to 89.8±1.5%, the risk of hypoxia is stated.
 Cartridge for automatic detection of analyte in body fluid sample and system comprising it / 2540424
Group of inventions refers to laboratory diagnostics. A device for the automatic detection of an analyte in a body fluid sample comprises an array of addressed analysis units for carrying out a chemical reaction, which generates a distinguishable signal carrying the information concerning the presence or absence of the analyte; an array of addressed reagent units referring to the individual addressed reagent unit, corresponding to the individual addressed analysis unit, and wherein the individual reagent unit is configured to be calibrated by a reference signal in the respective individual analysis unit before the arrays are assembled on the device; the individual analysis unit comprises a quantitative analysis tip having an inner surface carrying the reagents fixed on the surface for the analyte detection. The group of inventions refers to an embodiment of the above device additionally comprising a sampling unit, as well as to sub-systems comprising the above devices, methods for analysing using the above devices, a method for assembling the above devices and a method for sampling plasma from the blood sample in these systems.
 Method for assessing pulmonary blood flow / 2539718
Group of inventions refers to medicine, namely to pulmonology, cardiology, gerontology and sports medicine, and can be used for assessing the pulmonary blood flow by assessing the capillary pulmonary blood flow and intrapulmonary venoarterial bypass. That is ensured by measuring heart rate a minute, haemoglobin concentration (Hb g/l), total oxygen consumption (CO2(TOTAL) ml/min), arterial oxygen saturation (SART % or decimal portions 1) and mixed venous saturation in the greater circulation (GC) (Sv% or decimal portions 1). MBV (l/min) is calculated according to the measurements of Hb, CO2, SART, SV. Certain formulas are used to calculate the capillary pulmonary blood flow and intrapulmonary venoarterial bypass.
 Method of analysis of removal of biochemicals with meadow grass / 2538802
For analysis of removal with meadow grass of biochemicals the yield fluctuations are accounted depending on the structure of phytocenosis in the form of a ground cover. Conducting statistical data processing of testing the samples of grass from the test plots on the meander, central and terrace near flood plains, and also on the meadows with uneven and tiling location of grass species. And prior to establishment of sample plots the terrain reconnaissance is carried out with the grass cover selected for the measurements, an outline map of location of the components of the grass cover is made, then on each component of the grass cover in the form of a plot at least one temporary test plot is established, the wet and air-dry weight of the sample is determined by weighing on the cut grass sample. At that the tests on biochemical analysis are additionally carried out on the dried samples of grass to determine the concentration of at least three chemical nutrients in the form of mobile nitrogen, potassium and phosphorus oxides, and then by summing the concentrations of these three substances the total summarised removal of substances from the aerial part of grass on all test sites is calculated.
Method for assessing long-term hyperglycemia / 2538715
Invention represents a method for assessing long-term hyperglycemia based on measuring blood plasma glucose differing by the fact that glucose is measured twice: immediately after blood taking and 24 hours after blood plasma storage above a layer of blood corpuscles; that is followed by calculating a glucose reduction in percentage from the initial value which is used to assess long-term hyperglycemia with blood plasma stored at t=4.0±0.5°C.
Method for rapid analysis of cholesterol in immune complexes / 2538685
Invention can be used for the rapid analysis of cholesterol in immune complexes (CICs). Precipitated immune complexes containing multiply modified low-density lipoproteins of human blood serum are prepared by processing in buffer containing 10% PEG 3350, in ratio 1:3, incubating for 10 min at room temperature. The precipitated CICs are separated by centrifugation at 3100 g for 10 min at 23°C, diluted in PEG-free buffer; cholesterol is measured with the use of an enzymatic kit, and if the CICs content is more than 8.4 mg/dl, a high level is stated.
Method of treating recurrent acute myeloid leukaemia following transplantation of allogenic haemopoietic stem cells / 2538799
Invention can be used for treating recurrent acute myeloid leukaemia (AML) following the transplantation of allogenic haemopoietic stem cells. That is ensured by chemotherapy followed by administering (taking into account donor's leukocyte chimerism) donor's bone marrow lymphocytes in a combination with interleukin 2 (IL-2). The chemotherapy is conducted by the intravenous administration of anti-tumour preparations of cytabarine 100 mg/m2 twice a day for 7 days and indarubicine 12 mg/m2 1 time a day within 3 days. With underlying myelotoxic agranulocytosis with a granulocyte count of less than 0.5×109/l, the donor's bone marrow lymphocytes are transfused is a dose of 1×107 CD3+cells/kg of the patient's body weight that is followed by the intravenous drop-by-drop administration of IL-2 in a dose of 6 mln International Units 1-2 hours later. The 100% absence of the donor's leukocyte chimerism and any signs of the graft vs. host disease, the following two donor's lymphocyte transfusions and IL-2 infusions in a dose of 6 mln International Units are performed the first of the two donor's bone marrow lymphocyte transfusions is performed in a dose of 5×107 CD3+cells/kg, and the second one - in a dose of 1×108 CD3+cells/kg. The transfusion cycle makes 2-4 weeks. That is followed by therapeutic supporting course of IL-2 administration in a dose of 2 mln International Units a day for 5 days intravenously; the course cycle makes 4-5 weeks with the supporting therapy lasting for 2 years.
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FIELD: medicine. SUBSTANCE: invention can be used for the purpose of the early prediction of a risk of peripheral vitreochorioretinal dystrophies (PVCRD) on a paired eye after regmatogenous retinal detachment (RRD) surgeries. The IL18 content in the sub-retinal fluid (SRF) taken intraoperatively from an RRD eye is measured; if the measured value makes 550 pcg/ml, the PVCRD is predicted on the paired eye. EFFECT: invention enables the preventive treatment of the paired eye in case of the risk of the developing PVCRD before the clinical manifestations of the developing PVCRD in the individual with operated retinal detachment on the other eye. 6 ex
The present invention relates to ophthalmology, and can be used for early prediction of risk of progression of peripheral vitreoretinal dystrophies (PWHT) in the fellow eye after surgery for about regmatogenous retinal detachment (ROS). As you know, the peripheral vitreoretinal dystrophy (PWHT) are the main cause of the development regmatogenous retinal detachment (ROS), which in turn takes 2-3 place among all the possible reasons for the disability impaired (Libman E. C. Blindness, amblyopia and visual disabilities in the Russian Federation / E. C. Libman, E. C. Shakhova // Elimination of avoidable blindness. Global initiative who: materials Grew. Mezhregion, Symposium. - Ufa, 2003. - S. 38-42; Freund KB, Ciardella AP, Yannuzzi LA, Pece A, Goldbaum M, Kokame GT, Orlock D. Peripapillary detachment in pathologic myopia. Arch Ophthalmol. 2003 Feb; 121(2):197-204). Among all forms PWHT there are three dangerous development in Russia: lattice isolated retinal breaks, retinoschisis. According to the literature, lattice dystrophy cause GREW 13.9-35.0% of cases (Davis M. D. The natural history of retinal breaks without detachment. Arch Ophthalmol. 1974 Sep; 92(3):183-94.). Isolated retinal breaks as an independent type PWHT can lead to the development GREW 2.9% to 18% of cases, depending on their type: perforated, valve or breaks with lid (D. Robertson M, Norton E. W. "Lng-term follow-up of treated retinal breaks" Am J Ophthalmol. 1973 Mar; 75(3):395-404; Katie M. Williams, Mahmut Dogramaci, Tom H. Williamson, Retrospective study of rhegmatogenous retinal detachments secondary to round retinal holes, Eur J Ophthalmol 2012; 22(4):635-640). Retinoschisis (stratification of the retina) can lead to the development of ROS in the formation of gaps both walls or huge gap outer wall approximately 3.7-16% (Saksonova E. A., Paul, A., Nesterov, S. A. and other Retinoschisis and cysts in the retina // Ophthalmological journal. - 1975. - So 30. No. 3. - S. 173-176; Bezdetko P. A., Perednik Y. F., Sobolev, I. A., Shkil E. A. Cases of family retinoschisis in two generations // Ophthalmological journal. - 1991. No. 4. - S. 250-251; M. Shea, Shepens S. L. senile Retinoschisis type A clinical report of one hundred-seven cases // Arch. Ophthalmol. - 1960. - Vol.63. No. 8. - P. 1-9). It should be noted that hazardous PWHT occur quite frequently among the healthy population. Population frequency lattice dystrophy varies from 6.0 to 12.4 per cent, of retinal breaks from 3.8% to 18.0% and retinoschisis from 0.4 to 4.6% (Zakharov, Y. Clinical and genetic studies of peripheral vitreoretinal dystrophies and retinal detachment: Dis... Kida. the honey. Sciences. M., 1983; Shukia M. Ahuja O. P. A possible relation ship between lattice and snail track degeneration of the retina. - Amer. J. Ophthal. 1981. -Vol.92, No. 4. - R. 482-485). Meet PWHT in the fellow eye with ROS, and they lead to the development of retinal detachment is much more common than in the General population. Thus, according to Delaney WV Jr (Delaney, WV Jr, Oates RP. Retinal detachment in the second eye.Arch Ophthalmol. 1978 Apr: 96(4):629-34) hazardous PWHT lead to the development of ROS in 36% of cases. Asymptomatic atrophic gaps in the General population lead to the development of retinal detachment in 0-1,5% (Byer N. E. The natural histology of asymptomatic retinal breaks / N. E. Byer // Trams. Amer. Acad. Ophthal. Otolaiyng. 1982. - Vol.89, No. 9. - P. 1033-1039), and in patients with ROS and they lead to the development of retinal detachment in the fellow eye in 11% of cases (Davis M. D., Segal P. P., The natural course followed by the fellow eye in patients with rhegmatogenous retinal detachment, in R. C. Pruett, Retina Cong. New York, 1974. - P. 643-659). Everett W. G. (1963) even coined the term "Syndrome pair of eyes with retinal detachment", thereby stressing the severity of the dystrophic process with a high risk of GROWING up in the fellow eye. In General, this determines the relevance prediction of progression PWHT in the fellow eye in patients with ROS, including after their surgical treatment. To date, however, the task of predicting the risk of progression PWHT in the fellow eye after surgery about GROWING up as such is not applicable. Prediction of progression PWHT in the fellow eye in patients with ROS is an important task, as it can facilitate the timely laser photocoagulation of the retina, aimed at preventing the development of complications such as retinal detachment, as well as the development of new preventive approaches. Known methods for predicting the development PWHT, which are based either on the determination of the linear MSE of the spine of the blood vessels in the eye (patent RF №2189175 from 17.04.2001), either through biochemical studies (patent RF №2133036 from 15.10.1997), or by calculating theoretical probability PWHT (RF patent No. 2126225 from 02.04.1997). Also known a method for predicting the development of peripheral vitreoretinal dystrophies (PWHT) for myopia, consisting in studying the characteristics of metabolic processes in the layers of the eyeball, which by means of a laser analyzer of blood LACC-02" mode "Wavelet analysis to determine the parameters characterizing the microcirculation in the studied tissues of the eyeball, namely M - medium perfusion of blood, SO2- the average oxygen saturation of the blood microcirculation, PSH - indicator bypass, i.e., discharge of blood to bypass the existing microvasculature; and Vr - dimensional volume of tissue, and then determine the index of trophic membranes of the eye (PT) by the formula (RF patent No. 2423068 from 10.07.2011). In the light of modern ideas about the pathogenesis of proliferative changes in the structures of the eye seemed relevant immunological approach to solving this problem. Currently in the literature accumulated data on violations of systemic and local cytokine immune regulation in patients with regmatogenous retinal detachment (Razik, S. Prevention of progression of proliferative vitreoretinopathy after surgery for about rahmato the military retinal detachment: Dis. ... candles. the honey. Sciences. - M., 2005, Kuznetsova I. S. Prediction and early diagnosis of progression to proliferative vitreoretinopathy after successful surgical treatment regmatogenous Otani retina: Dis. ... candles. the honey. Sciences. - M., 2012). A known method for predicting the progression of proliferative vitreoretinopathy and the occurrence of recurrent retinal detachment after surgery about regmatogenous retinal detachment, based on the determination in subretinal fluid obtained during the surgical intervention, polyfunctional cytokine - tumor necrosis factor alpha (TNFα). When the content TNPα equal to or above 250 PCG/ml, predict a high probability of recurrence of retinal detachment [Slepov O. S., Zakharov, Y., Razik, S. a Method for predicting the recurrence of retinal detachment after surgery about regmatogenous retinal detachment (RF patent for invention 2307355 from 27.9.2007) . In recent years, thanks to the improvement of teaching skills and capacity testing of cytokines in small volumes of biological fluids, in particular, in samples of subretinal fluid (Sri), possibility of simultaneous determination of the concentrations of multiple cytokines in a single sample, that is, multiplex analysis. Our attention was attracted by interleukin 18 (IL18). This C is taken is synthesized by macrophages and other cells of the body, is an activator of cells of the monocyte - macrophage series, has Pro-inflammatory properties, is involved in the initiation of apoptosis. Production of IL18 induced stress signals (neurogenic and bacterial origin). In a series of immunoregulatory mediators IL18 occupies a special position, as it is one of the key cytokines of the formation of innate and acquired immunity, differentiation and functional activity of macrophages, dendritic cells and T-lymphocytes. IL18 stimulates the production of IFNγ, GM-CSF, TNF, IL1, IL2, adhesion molecules and apoptosis factors that contributes to the activation of cytotoxic T-lymphocytes, NK-cells and the formation of an effective anti-infective and anti-tumor immune response. However, since IL18, like many other immunoregulatory cytokines, has pleiotropically, its biological effects in some cases may be undesirable or unpredictable. For example, in the literature there are contradictory data on the ability of IL18 to stimulate T-helper 1-type or T-helper 2-type; use of recombinant IL18 with infectious or malignant processes leads to stimulation of the production of cytokines and adhesion molecules that along with stimulating effect on the formation of protective monogo response can have negative consequences, because this protein can cause the development of inflammation. In addition, IL18 can stimulate differentsirovku T helper 2-type, producing IL4, which can also ambiguous influence on the formation of the cellular immune response. In General, it should be noted that due to the presence of such diverse effects, IL18 is involved not only in the defense reactions of the body, but also in the pathogenesis of many diseases involving chronic inflammation and destruction of tissues. Shown the involvement of IL18 in the mechanisms of formation of such pathological conditions as Crohn's disease, rheumatoid arthritis, metabolic syndrome, allergies, diabetes, atherosclerosis and other [Yakushenko E. C. Interleukin-18: biological effects and prospects for clinical application. Diss. Prof. the honey. Sciences, 2012]. In the aspect of eyes diseases IL18 mentioned in the literature are very rare, particularly in connection with retinopathy of prematurity, as one of the possible factors influencing the development of late complications. We hypothesized that IL18 may play a role in the development of videoproiettore process with the formation of PWHT in patients with idiopathic retinal detachment. The objective of the proposed method is to develop informative immunologic approach in predicting progression PWHT on porn the m eye in patients with ROS. The technical result of this method is the ability to schedule preventive treatment in the fellow eye at the risk of progression PWHT for a few weeks or months before the appearance of clinical signs of progression PWHT in individuals with operated retinal detachment in the other eye. The technical result is achieved due to the definition in Sri certain level IL18 after surgery for detachment of the retina. Pathogenetic substantiation of the proposed method based on the data obtained during the survey of 19 patients (11 women and 8 men) with ROS on one eye and PWHT in the fellow eye. The age of patients between 21 and 65 years. All 19 patients performed on one eye of the same type of operation for ROS - episcleral sealing silicone sponge with releasing subretinal fluid (SRG). All patients in the fellow eye were characterized by degenerative changes in the peripheral retina in the form of different types PWHT. All patients a few days after operation in occasion of ROS was performed LK retina pair of eyes about PWHT according to standard techniques. All patients performed a multiplex quantitative content analysis of cytokines in the SRF, as well as obtained during surgery about GROWING up. In each sample SRG determined simultaneously 16 cytokines, FPIC is service to show various biological effects: interleukins (IL1β, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17, IL18), factors of tumor necrosis (TNFα, TNFβ), interferons (IFNα, IFNγ), a growth factor, vascular endothelial (VEGF-A), transforming growth factor (TGF-β1). The study was conducted on a flow cytometer (BD FACSCanto II) in the BD FACSDiva, using sets of CBA (BD Biosciences, USA); data processing - program FCAP Array (SoftFlow, USA). The observation of patients in the dynamics ranged from 1 month to 1 year after surgery. Retrospectively, depending on the outcome of the operation and the postoperative period, the patients were divided into two groups. In the I-th group consisted of 16 people with the fit of the retina in eyes operated on for ROS. The majority of these patients (14 people) a few days after surgery, about GREW conducted local laser coagulation (LK) lattice degeneration of the retina in the fellow eye; 2 people the need for LC in the fellow eye with PWHT was not. During the postoperative observation in dynamics in 12 of 16 status of the retina fellow eyes remained stable, without signs of progression PWHT, with the following follow-up period: 1 month - 1, 3 months 2 - 6 months 3 more than 1-2 years to 6 people. 4 patients detected signs of progression of the pathological process in the fellow eye: the appearance of new areas of lattice degeneration of the retina (however is on preventive local LK) 2 weeks (2 cases) or 1 month (2 cases) after surgery on the eye with ROS. In the II-th group included 3 patients with incomplete adherence of the retina after surgery. All three of them in a few days after surgery, about GROWING up was conducted local LK about PWHT. During the postoperative observation the dynamics of 1 patient condition pair of eyes remained stable (the follow - up period 6 months), and in 2 patients experienced progression of the pathological process in the fellow eye: the appearance of new areas of lattice degeneration, recorded 2 months after surgery on the eye with ROS. Retrospective analysis showed that in the vast majority of the studied samples SRG were found IL18 and TGFβ1 (100%), IL6 (94%) and IL8 (89%), VEGF-A (68%), IL2 and IFNγ (no 63%); almost half of the cases were detected IFNα (47%), IL10 (42%), IL5 (41%), about one third - and IL12 (37%) and TNFα (32%). Only in some cases in Sri was found IL4 (10.5%) and almost never IL1β, IL17 and TNFβ. This suggests the predominance of a chemotactic (IL8) and regulating proliferative processes factors (TGF-β, IFNα), and mediators of the immune" inflammation (IFNγ) and cytokines, in varying degrees, affect the development of inflammatory, autoimmune and angiogenic reactions (IL2, IL6, IL18, VEGF). However, "key" Pro- (TNFα, TNFβ, ILIβ) and anti-inflammatory (IL4, IL10) cytokines were detected much less frequently or were missing. Retrospective analysis of p is Casali, that the local imbalance of cytokines in the eye with ROS, defined in the study of SRF, as well as may have an impact on the outcomes of surgical treatment in fact, the eyes, and in the course of pathological process in the fellow eye with PWHT. In General, this is consistent with the known phenomenon friendly immunological reactions observed in the study of different forms of eyes diseases. The worst manifestation of this imbalance appears to be impaired immunoregulation weakening immunosuppressive, antiproliferative factors and a shift towards greater chemotactic reactions and immune inflammation. However, specific criteria of adverse prognosis can serve based on our observations, the quantitative content of IL18 in Sri. A comparative analysis of the content of IL18 in SRE in patients with clinical signs of progression PWHT in the fellow eye (only 6 cases, including 4 - for successful operations for ROS and 2 under adverse outcomes) and absence (total of 13 cases, including 12 - when successful operations for ROS and 1 - under unfavorable outcome), all patients were divided into subgroups depending on the absolute levels of this cytokine in Sri the operated eye. If this were allocated to patients with high (>1000 PG/is l; n=3), medium (from 550 to 1000 PG/ml; n=5) and low (less than 500 PG/ml; n=11) levels of IL18. Retrospective analysis showed that patients with levels of IL18 in SRF, as well as in excess of 550 PG/ml, progression PWHT in the fellow eye was noted in 6 of 8 cases (75%), whereas at lower levels of IL18 in Sri (n=11) of these cases were not identified (difference statistically significant, p=0.01). The method is as follows. During surgery on the eye with regmatogenous retinal detachment take SRG and determine its content of interleukin 18 (IL18). When a level exceeding 550 PG/ml predicts early progression PWHT in the fellow eye. Clinical examples Example 1. Patient K., 59 years old. He enrolled in the Department of pathology of the retina IRI GB of them. Helmholtz diagnosed with OU - high myopia, complicated PWHT. OS - Subtotal high retinal detachment with two valve breaks. OD: VIS=0,09 sph -9,0 d cyl-0.75 in ax 60=0,7; IOP-19 mm RT.article. OS: VIS=0,02 sph -3,0 d=0,2; IOP=19 mm RT.article. OU - calm. The transparent cornea, anterior chamber, the average depth. The structural iris, lens - transparent, vitreous body - filamentous destruction. The fundus of the eye: OU - optic disc pale pink, clear boundaries. OD - on the periphery of the multiple areas of chorioretinal dystrophy, in the upper-outer quadrant area of massive the th hyperpigmentation; OS - Subtotal high retinal detachment with coarse folds and the valve breaks, respectively 4 and 5 o'clock, 3 o'clock - thinning of the retina. Surgery on OS - sealing silicone sponge with releasing Sri. Retin-lay. In the fellow eye OD after 4 days after surgery produced LK retina around the areas of lattice degeneration. Immunological studies SRG OS detected level IL18 - 1685 PCG/ml After 1 month follow-up patient: OS - without negative dynamics; in the fellow eye OD was diagnosed with a new area PWHT, in this regard, an additional laser photocoagulation. This example shows that at a high level of IL18 in Sri the operated eye (1685 PCG/ml) in patients with a favorable outcome surgical treatment about GROWING up in the fellow eye, despite the LK occurred progression PWHT. Example 2. Patient M., 55 years. He enrolled in the Department of pathology of the retina IRI GB of them. Helmholtz diagnosed with OU - mild myopia, complicated PWHT. OS - Subtotal high retinal detachment with a gap. OD: VIS=0,6 sph - 0,5 d=0,8; IOP=16 mm RT.article. OS: VIS=0,05 n/a; IOP=18 mm RT.article. OU - calm. The transparent cornea, anterior chamber, the average depth. The structural iris, lens - opacity cortical layers and under the rear kapsa is Oh, the vitreous body is a filamentous destruction. The fundus of the eye: OU - optic disc pale pink, clear boundaries. OD on the periphery in the upper half of an extensive zone of lattice dystrophy with two perforated breaks; OS - Subtotal high retinal detachment with multiple breaks in the area of lattice dystrophy. The operation took place on OS - sealing silicone sponge with releasing Sri. Retin-lay. In the fellow eye OD after 4 days after surgery conducted LK retina around the areas of lattice degeneration. Immunological studies Sri identified from OS level IL18 - 503 PCG/ml At the time of observation in the dynamics within 1 year late complications on the operated eye - OD revealed no signs of progression PWHT in fellow eye - OS is not marked. Example 3. Patient K., 25 years. He enrolled in the Department of pathology of the retina IRI GB of them. Helmholtz diagnosed with OU - high myopia, complicated PWHT. OD - total high retinal detachment with gaps. OD: VIS=0,09, sph - 7,0 l=0,1; IOP=17 mm RT.article. OS: VIS=0,1 sph - 6,0 d=0,8-0,9; IOP-15 mm RT.article. OU - calm. The transparent cornea, anterior chamber, the average depth. The structural iris, lens - transparent, vitreous body - filamentous destruction. The fundus of the eye: OU - optic disc pale pink, the myopic staphyloma, OD - total high detachment CE is chutki with multiple breaks on the background of lattice dystrophy, respectively 7, 9, 12 o'clock, coarse fibrous changes in the retina. OS is on the periphery of the lattice dystrophy, respectively, 12 and 5 hours. The operation took place on OD - sealing silicone sponge with releasing Sri. Retin-lay. In the fellow eye - OS 3 days after surgery conducted LK retina around the areas of lattice degeneration. Immunological screening SRG of the OD level IL18 was 35 PCG/ml At the time of observation in the dynamics within 2 years of the late complications of the operated eye - OD revealed no signs of progression PWHT in fellow eye - OS is not marked. Clinical examples No. 2 and 3 show that when the level of IL 18 in Sri operated eye within 35-503 PCG/ml in patients with a favorable outcome surgical treatment about the GROWING evidence of progression PWHT on paired the eyes with 1-2 years of observation after surgery have been identified. Example 4. Patient K., 37 years. He enrolled in the Department of pathology of the retina IRI GB of them. Helmholtz diagnosed with OU - myopia of an average degree, complicated PWHT. OD - extensive high retinal detachment with gaps. OD: VIS=0,05 sph - 4,5 d=0,6; IOP=15 mm RT.article. OS: VIS=0,05 sph - 5,5 d=0,8; IOP=16 mm RT.article. OU - calm. The transparent cornea, anterior chamber, the average depth. The structural iris, lens - transparent, vitreous body - filamentous destruction. The fundus of the eye: OU - discretelog nerve pale pink, boundaries clear, OD - extensive high retinal detachment with fibrotic changes and perforated rupture respectively 3 hours. OS is on the periphery in the outer half of the deposition of pigment. The operation took place on OD - sealing silicone sponge with releasing Sri. Retin-lay. On OD 3 days after surgery was additionally conducted LK; fellow eye - OS. LK retina did not. Immunological screening Sri OD IL18 level is below the sensitivity of the test (less than 5.0 PCG/ml). At the time of observation in the dynamics within 1 year late complications on the operated eye OD revealed no signs of progression PWHT in the fellow eye OS is not marked. Clinical example # 4 shows that in the practical absence of IL 18 in Sri the operated eye of a patient with a favorable outcome surgical treatment about GROWING up without holding LK pair eye signs of progression PWHT in the fellow eye during the observation period of 1 year have not been identified. Example 5. Patient L., 50 years. He enrolled in the Department of pathology of the retina IRI GB of them. Helmholtz diagnosed with OU - myopia of an average degree, complicated PWHT. OS - fresh top extensive high retinal detachment with gaps. OD: VIS=0,05 sph - 5,5 l=1,0; IOP-18 mm RT.article OS: VIS=0,02 sph - 8,0 l=0,3, IOP-17 mm RT.article. OU - calm. The cornea is a transparent, front cameraready depth. The structural iris, lens - soft dimness of the cortical layers, the vitreous body - filamentous destruction. The fundus of the eye: OU - optic disc pale pink, clear boundaries, OD - on the periphery of the top multiple areas of lattice degeneration ("snail trail" and the classic form). OS - top of the extensive high retinal detachment with two valve gaps of size 1 and 2 RD respectively 1 and 2.30 o'clock on the background of classical lattice dystrophy. Surgery on OS - sealing silicone sponge with releasing Sri. During the operation developed partial hemophthalmus from the vessel through the valve gap. After surgery remained local retinal detachment in the upper-outer quadrant. After 2 months the second operation - circles silicone band, Subtotal vitrectomy with intravitreal the introduction of PFOS and then deleting. The retina does not lay down. In the fellow eye OD 3 days after the first surgery laser coagulation of the retina around the areas of lattice degeneration. Immunological screening SRG OS received during the first operation, the detected level IL18 - 15 PCG/ml Dynamic observation within 1.5 years of progression PWHT in the fellow eye OS not found. This example shows that with a low level of IL18 in Sri opererating the eye (15 PCG/ml) in patients with unfavorable outcome of surgical treatment regarding ROS, as described above, patients with a favorable outcome (examples No. 3 and No. 4), progression PWHT in the fellow eye was not observed. Example 6. Patient W., 55 years. He enrolled in the Department of pathology of the retina IRI GB of them. Helmholtz diagnosed with OU - myopia of an average degree, complicated PWHT. OS - funnel retinal detachment with a gap. OD: VIS=0,1 sph - 4,5 d=0,5; IOP-15 mm RT.article OS: VIS=0,02 n/a, IOP=10 mm RT.article OU - calm. The transparent cornea, anterior chamber, the average depth. The structural iris, lens - gentle haze in cortical layers and under the posterior capsule, vitreous body is expressed destruction. The fundus of the eye: OU - optic disc pale pink, clear boundaries. OD - expressed retinoschisis outer half with coarse pigment deposition, respectively 10.30 o'clock perforated gap 1.5 RD with pigment. OS - funnel retinal detachment with valve gap, respectively, 15 hours, size RD, areas intraretinal fibrosis and lattice dystrophy, respectively 11-13 hours. Surgery on the OS. the 1st phase of the operation - sealing silicone sponge with releasing Sri and intravitreal the introduction of PFOS. 10 days - 2nd stage surgery - Subtotal vitrectomy with removal of PFOS. The operation went smoothly: the retina over a large lay. 4 days after the 2nd stage of the operation was about Sweden LUX at OU around gaps and areas PWHT. 3 months later, the patient had complained of sudden loss of vision OS. When the survey was diagnosed with a recurrence of retinal detachment on the OS and the emergence of new areas PWHT on OD. Immunological screening SRG OS received during the first operation, the detected level IL18 - 698 PCG/ml This example shows that at a high level of IL18 in Sri the operated eye (698 PCG/ml) in patients with unfavorable outcome of surgical treatment about GROWING up, in the fellow eye, in spite of Luke, has been a progression PWHT. Thus, the method provides the detection of the risk of progression PWHT in the fellow eye after surgery about GROWING up with both favorable and unfavorable outcome, which can help to determine the best tactics dispensary observation of patients and timely implementation of preventive measures, including immunological orientation, with the subsequent control. The method of early prediction of progression of peripheral vitreoretinal dystrophies (PWHT) in the fellow eye after surgery about regmatogenous retinal detachment (ROS) on the other eye, characterized in that subretinal fluid (SRG) obtained during surgery on the eye with ROS, examine the contents IL18 and if it is the RAM more than 550 PCG/ml predict the progression PWHT in the fellow eye.
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