Method for assessing disturbed haemoglobin oxygenation with reduced microviscosity of peripheral erythrocyte membrane of pregnant women in third trimester of gestation and aggravated cytomegalovirus infection |
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IPC classes for russian patent Method for assessing disturbed haemoglobin oxygenation with reduced microviscosity of peripheral erythrocyte membrane of pregnant women in third trimester of gestation and aggravated cytomegalovirus infection (RU 2540425):
 Cartridge for automatic detection of analyte in body fluid sample and system comprising it / 2540424
Group of inventions refers to laboratory diagnostics. A device for the automatic detection of an analyte in a body fluid sample comprises an array of addressed analysis units for carrying out a chemical reaction, which generates a distinguishable signal carrying the information concerning the presence or absence of the analyte; an array of addressed reagent units referring to the individual addressed reagent unit, corresponding to the individual addressed analysis unit, and wherein the individual reagent unit is configured to be calibrated by a reference signal in the respective individual analysis unit before the arrays are assembled on the device; the individual analysis unit comprises a quantitative analysis tip having an inner surface carrying the reagents fixed on the surface for the analyte detection. The group of inventions refers to an embodiment of the above device additionally comprising a sampling unit, as well as to sub-systems comprising the above devices, methods for analysing using the above devices, a method for assembling the above devices and a method for sampling plasma from the blood sample in these systems.
 Method for assessing pulmonary blood flow / 2539718
Group of inventions refers to medicine, namely to pulmonology, cardiology, gerontology and sports medicine, and can be used for assessing the pulmonary blood flow by assessing the capillary pulmonary blood flow and intrapulmonary venoarterial bypass. That is ensured by measuring heart rate a minute, haemoglobin concentration (Hb g/l), total oxygen consumption (CO2(TOTAL) ml/min), arterial oxygen saturation (SART % or decimal portions 1) and mixed venous saturation in the greater circulation (GC) (Sv% or decimal portions 1). MBV (l/min) is calculated according to the measurements of Hb, CO2, SART, SV. Certain formulas are used to calculate the capillary pulmonary blood flow and intrapulmonary venoarterial bypass.
 Method of analysis of removal of biochemicals with meadow grass / 2538802
For analysis of removal with meadow grass of biochemicals the yield fluctuations are accounted depending on the structure of phytocenosis in the form of a ground cover. Conducting statistical data processing of testing the samples of grass from the test plots on the meander, central and terrace near flood plains, and also on the meadows with uneven and tiling location of grass species. And prior to establishment of sample plots the terrain reconnaissance is carried out with the grass cover selected for the measurements, an outline map of location of the components of the grass cover is made, then on each component of the grass cover in the form of a plot at least one temporary test plot is established, the wet and air-dry weight of the sample is determined by weighing on the cut grass sample. At that the tests on biochemical analysis are additionally carried out on the dried samples of grass to determine the concentration of at least three chemical nutrients in the form of mobile nitrogen, potassium and phosphorus oxides, and then by summing the concentrations of these three substances the total summarised removal of substances from the aerial part of grass on all test sites is calculated.
Method for assessing long-term hyperglycemia / 2538715
Invention represents a method for assessing long-term hyperglycemia based on measuring blood plasma glucose differing by the fact that glucose is measured twice: immediately after blood taking and 24 hours after blood plasma storage above a layer of blood corpuscles; that is followed by calculating a glucose reduction in percentage from the initial value which is used to assess long-term hyperglycemia with blood plasma stored at t=4.0±0.5°C.
Method for rapid analysis of cholesterol in immune complexes / 2538685
Invention can be used for the rapid analysis of cholesterol in immune complexes (CICs). Precipitated immune complexes containing multiply modified low-density lipoproteins of human blood serum are prepared by processing in buffer containing 10% PEG 3350, in ratio 1:3, incubating for 10 min at room temperature. The precipitated CICs are separated by centrifugation at 3100 g for 10 min at 23°C, diluted in PEG-free buffer; cholesterol is measured with the use of an enzymatic kit, and if the CICs content is more than 8.4 mg/dl, a high level is stated.
Method for prediction of risk of squamous metaplasia in respiratory bronchial epithelium with detected isolated basal cell hyperplasia / 2538642
Invention refers to medicine, specifically to oncology, and concerns a method for the prediction of a risk of squamous metaplasia in the respiratory bronchial epithelium with detected isolated basal cell hyperplasia. The ssubstance of the method: positive nuclear expression of the Ki67 proliferation marker and membrane differentiation expression of CD 138 squamous epitheliocytes in the bronchial epithelium are measured. If the expression level Ki67≥25%, and CD138 is ≤3.5%, the risk of metaplasia in the respiratory bronchial epithelium is predicted.
Method of predicting efficiency of neoadjuvant chemical therapy in patients with resectable basal-like triple-negative breast cancer / 2538638
Invention relates to field of medicine, namely to oncology, and can be used to predict efficiency of neoadjuvant chemical therapy (NACT) in patients with resectable basal-like triple-negative breast cancer. NACT efficiency in patients is calculated by formula: P=eY/(1+eY), where P is value of probability of sign development, Y is value of regression equation; e is mathematical constant, equal 2.72. Value of regression equation is determined by formula: Y=(42.8-1.86X1-9.3X2-2.14X3+3.19X4), where (42.8) is value of coefficient of free term regression; X1 represents subtypes of basal-like triple-negative cancer ( 1 - subgroup with expression of only CK 5/6; 2 is subgroup with expression of only EGFR1; 3 is subgroup with expression of CK 5/6 and EGFR1), (1.86) is the value of said sign regression coefficient; X2 is the level of EGFR1 expression in biopsy material (1 is high level, 2 is low level), (9.3) is the value of said sign regression coefficient; X3 is the level of expression of Ki-67 in biopsy material (1 is low level, 2 is high level), (2.14) is the value of said sign regression coefficient; X4 is chemical therapy scheme (1-FAC, 2-CAX), (3.19) is the value of said sign regression coefficient. If P<0.5, low efficiency of NACT is predicted, if P>0.5, high efficiency of NACT is predicted.
 Method for confirmation of death caused by ventricular fibrillation accompanying myocardial infarction / 2538622
For the purpose of the confirmation of a patient's death caused by ventricular fibrillation accompanying myocardial infarction in the patients died from myocardial infarction in the course of autopsy on the basis of a macroscopic pattern, the pathological process age is determined. This enables selecting examination areas in the heart at the level of necrosis. Absolute values (AV) of the following cell populations are counted in the x600-magnified visual field with using haematoxylin and eosin: necrosis-borderline lymphocytes (Lfb) in myocardial infarction of the age of 1-2 days, necrosis neutrophilic granulocytes (NGn) and fibroblasts (Fbn) in myocardial infarction of the age of 3-5 days. The derived absolute values are compared to threshold values (TV) with the threshold values of Lfb=2, NGn=38, Fbn=1. Each visual field is assigned with a diagnostic coefficient (DC), which is equal to 1.9 at the age of myocardial infarction of 1-2 days, if the absolute values Lfb<2 or -3.9, Lfb>2; if the age of myocardial infarction is 3-5 days, the diagnostic coefficient is -2.1, if AFbn<1, or the diagnostic coefficient is 7.2, if the absolute value of Fbn>1; the diagnostic value of 1.36 is shown by the absolute value of NGn<38; the diagnostic value is -11, if the absolute value is NGn>38. The total value (ΣDC) is calculated by moving between the visual fields within the examined area, and if ΣDC>12.78, the patient's death is stated to have come from ventricular fibrillation; if ΣDC<-12.78, the death is stated to be caused otherwise.
Method for prediction of dental implantation effect at planning stage / 2538087
Invention refers to medicine and aims at predicting potential negative effects of the dental implantation. The method involves examining a bone tissue by a histological examination of bone chips sampled from an implant bed preparation place, detecting the morphological changes of the bone tissue within the expected dental implantation, measuring a diameter of Haversian canals, a thickness of bone rods, an gluing line, studying a proliferation reaction of mesenchymal cells accompanied by no inflammation, the presence of collagen fibres, disturbed spongy layer architectonics, an intensity of bone blood supply, fibrosis and hyalinosis of a vascular wall, the presence or absence of rheological disorders, the presence or absence of osteoid depositions, and the presence or absence of inflammations. If the morphological criteria characterizing the creation processes are observed to dominate over those characterizing the destruction processes, the bone tissue state within the expected dental implantation is considered to be good, and a positive result of the operation is predicted.
Device and method for selection and preservation of biosamples in extreme living conditions / 2537174
Filter is placed in a separate holder with a hole, a sample is applied on an open part of the filter and after sampling the filter is left in the holder to complete the sample drying. Before sampling, analytic aerosol bilateral filters AFA-X are opened and taken out with pincers from packs of the ZipLock type. The filter is placed on a lower plate of the holder with its working fleecy side towards the hole in an upper plate of the holder. Viniplast holders are used and sampling on the open part of the filter is performed. After the sample drying the filter is removed from the holder, placed in a separate ZipLock pack and kept at the temperature of the environment. The device contains filters, placed in the holder, namely two analytic aerosol bilateral filters AFA-X with one fleecy side and two filter holders from the sheet viniplast, made with a possibility of their fixation to a working table. Each holder consists of rectangular plates - upper and lower, with rounded edges and a hole in the centre, and is provided with a textile fastener from nomex, made from two parts - hook one and loop one.
Method for predicting hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis / 2244303
One should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
 Method for predicting prostatic tuberculosis / 2244513
One should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
 Device and method for extracting nucleic acids / 2244559
The suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
Method for assay of medicinal sensitivity of tuberculosis mycobacterium / 2244927
Diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
 Endogenic pharmaceutical composition prepared on basis of goal-seeking activation of humoral mediators of brain cortex nerve ending / 2244928
Invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
 Method for estimating treatment effectiveness in atherosclerotic lower extremity ischemia patients / 2244929
Method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
 Method for predicting the result of acute peroral intoxication with cauterizing liquids / 2244930
At patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
 Method for detecting disease duration in children with chronic obstructive pyelonephritis (variants) / 2245548
Disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
 Method for predicting prostatic cancer / 2245549
The present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula:
Nutrient medium for accumulation of cell sample for following cytological and/or immunocytochemical analysis / 2246110
Invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
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FIELD: medicine. SUBSTANCE: anti-cytomegalovirus antibody titre, microviscosity of erythrocyte membranes are measured in the peripheral blood by eximerisation of a lipid bilayer and a protein-lipid layer; the number of degenerative forms of erythrocytes, target-like erythrocytes and echinocytes, oxyhaemoglobin content are also measured. If the cytomegalovirus antibody titre is 1:1600, decreased eximerisation of the lipid bilayer to 0.59±0.006 FE/FM, of the protein-lipid layer to 0.70±0.004 FE/FM, increased number of the degenerative forms of erythrocytes to 10.5±0.3%, of the echinocytes to 8.0±0.5%, of the target-like erythrocytes to 11.5±0.9%, reduced oxyhaemoglobin to 89.8±1.5%, the risk of hypoxia is stated. EFFECT: more accurate assessment.
Membranes of erythrocytes in violation of the metabolism in the body are exposed to peroxide oxidation [2, 4, 6]. When exposed on the erythrocyte membrane of immune disorders is a reorganization of the protein-lipid structure of the membrane, leading to changes in intracellular metabolism [10]. Exposure to infectious factors in various diseases lead to the restructuring of the phospholipids in the membranes of erythrocytes and increased their microviscosity[1, 3, 4, 5]. These changes in erythrocyte membranes affect functional processes, manifested in violation of the oxygen transport function of erythrocytes [7, 9]. The purpose of this study is to develop a method of evaluating changes in the oxygenation of hemoglobin in erythrocytes in aggravation in pregnant women during the third trimester of gestation cytomegalovirus infection, which leads to decrease in microviscosity of erythrocyte membranes, creating the formation of a hypoxic condition. In the available literature is not found description action cytomegalovirus infection on the microviscosity of erythrocyte membrane and its indirect effects on the oxygenation of hemoglobin. The claimed method has the following methods: 1. Surveyed 45 pregnant on the period of gestation 20-22 weeks of age 18-25 years with chronic cytomegalovirus infection (current cytomegalovirus infections in herds and exacerbation (main group) and 15 pregnant at the same gestation periods without pathology (control group). Pregnant symptomatically the current cytomegalovirus infections manifested as acute respiratory diseases rhinopharyngitis. Clinical diagnosis of exacerbation of the current cytomegalovirus infections was established in the comprehensive study of peripheral blood for the presence of IgM or a fourfold or more increase of the titer of IgG antibodies in paired sera in the dynamics after 10 days, the avidity index is more than 65%, and CMV DNA. The studies were conducted with regard to the requirements of the Declaration of Helsinki of the world medical Association's "Ethical principles of research with regard to human amended 2000 and the rules of clinical practice in the Russian Federation, approved by order of the Ministry of the Russian Federation No. 266 dated 19.06.2003, Verification of CMV, the definition typespecification antibodies, the avidity index was carried out by the methods of ELISA spectrophotometer Stat Fax 2100" (USA) using test systems CJSC "Vector-best" (Novosibirsk); detection of CMV DNA PCR methods, apparatus DT-96 using sets NGOs "DNA-technology" (Moscow). 2. The content of oxyhemoglobin was determined by the method of Evelyn and Malloy [8]. 3. The erythrocytes in the peripheral blood were determined using an automated flow cytometric installation Mekos" (Moscow). 4. Measurement of microviscosity of erythrocyte membranes was performed lateral to the maps in the AI hydrophobic fluorescent probe pyrene. Definition of microviscosity based on the formation of axieros (active dimers) pyrene in the lipid environment [2, 3]. The fluorescence of pyrene was measured spectrofluorometrically on spectrofluorimeter Hitachi (Japan). To determine the lipid bilayer found the intensity of the fluorescence or luminescence of pyrene at a wavelength of 334 nm excitation, the wavelength of the monomer - 395 nm, the wavelength of exameron - 470 nm. To determine microviscosity zone lipid-protein contacts wavelength excitation - 286 nm, the wavelength of the monomer - 395 nm, the wavelength of exameron - 470 nm. Evaluation of microviscosity is based on the calculation of the coefficient of exibilization pyrene (KEX=F470/F395), which is equal to the ratio of fluorescence intensity of exameron to the intensity of fluorescence of the monomers. The coefficient of exibilization is inversely proportional to microviscosity and is denoted as FE/FM. In the research the following data were obtained: 1. There have been changes to microviscosity of erythrocyte membranes. With acute cytomegalovirus infection in the third trimester of gestation until the titer of antibodies to cytomegalovirus 1:1600 ratio aximization pyrene, defines the microviscosity, amounted in the lipid bilayer of the erythrocyte membrane 0,59±0,006 FE/FM(control - 0,86±0,003 FE/FM ), and in protein-lipid layer to 0.70±0,004 FE/FM(control - 1,1±0,005 FE/FM). 2. Increasing microviscosity in erythrocyte membranes was reflected in their structure. Using the automated flow cytometric installation Mekos" it was found that pregnant women who have had an exacerbation of cytomegalovirus infection number of discocytes in the peripheral blood decreased to 70.0±1,3% (control of 91.3±1,7%); the Content of echinocytes increases to 8.0±0,5% (control - 2,7±0,09%); machinewide erythrocyte - to 11.5±0,9% (control - 2,0±0,05%), and the number of degenerative forms of 10.5±0.3% (control - 4,0±0,03%). The content of oxyhemoglobin in the blood of pregnant women undergoing an exacerbation of cytomegalovirus infection in the third trimester of gestation with the titer of antibodies to cytomegalovirus 1:1600 declined to 89.8±1,5% (control of 97.8±2,1%). Thus, exacerbation of cytomegalovirus infection in the third trimester of gestation with a titer of 1:1600 increases microviscosity membranes of red blood cells, reducing the content of oxyhemoglobin to 89.8±1.5% at the control of 97.8±2,1%, threatening the formation of hypoxia. Literature 1. Gorchinskaya I. A., Glotin L. Yu, Throat E. I., Rowe T. A., Borduchkov Y. N. Changing microviscosity membranes of lymphocytes and red blood cells in cancer patients // Issues of honey. chemistry. - 1999. - So 45. No. 1. - S. 53-57. 2. Zhuchkov, S., Casanova H. O., T is Rina A. E. Lipid peroxidation and antioxidant protection of cell membranes in diabetes mellitus // Institute of medico-biological problems of VSC RAS and the Government of Alanya. - 2017. - So 20, No. 36 - S. 7-12. 3. Dobretsov, that is, the Fluorescent probes in the study of cells, membranes and lipoproteins // M.: Nauka, 1989. - S. 206. 4. Dubinin E. E., PuTTYgen A. C. Oxidative modification of proteins, its role in pathological States // Ukr. biochem. Journe. - 2008. - So 80, No. 6 - N-8-10. 5. Ishutin N. A., Dorofeenko N. N., Andrievsky, I. A., Dobrikova I. C., Boleslava S. M. Changing microviscosity membranes of red blood cells in pregnant women infected with herpes virus, Byull. Fiziol. and Patel. Respiration SB RAMS, Blagoveshchensk, 2006. - Vol.23 (Appendix). - S. 16-18. 6. Kopytova T. C., Chemchina L. N., Suzdaltsev I. C. Oxidative stress, anoxemia in patients with severe widespread dermatitis // Modern problems of dermatology, immunology and medical cosmetology. - 2009. No. 2. - S. 10-13. 7. Lutsenko MT, Andrievsky I. A. Method of predicting the stability of erythrocyte membranes during exacerbation of herpes-virus infection in pregnant // Medical Informatics. - 2009. - №1(19). - S. 27-30. 8. Pokrovsky A. A. Biochemical research methods // directory of M: "Medicine", 1969. - S. 337. 9. Ryabov, A., Pasechnik I. N., Azizov, J. M. Activated oxygen species and their role in some Pat the logical States // Anesthesiology. - 1991. No. 1. - S. 63-69. 10. Bishop Ch. Overall red cell metabolism. In: The red blood cell. Ed. Bishop Ch. and Surgenor D. New York, 1964, p. 147. The method of evaluation of the threat of the formation of hypoxia in pregnant women with acute exacerbation of cytomegalovirus infection in the third trimester of gestation, characterized by the fact that in the peripheral blood to determine the titer of antibodies to cytomegalovirus, the microviscosity of erythrocyte membranes by aximization lipid bilayer and protein-lipid layer, the number of degenerative forms of erythrocytes, machinewide erythrocytes and echinocytes, the content of oxyhemoglobin and the titer of antibodies to cytomegalovirus 1:1600, identifying reduce aximization lipid bilayer to 0,59±0,006 FE/FMprotein-lipid layer to 0.70±0,004 FE/FM, increasing the number of degenerative forms of erythrocytes to 10.5±0.3%, echinocytes to 8,0±0,5%, machinewide erythrocytes to 11.5±0.9 per cent, the decrease in the content of oxyhemoglobin up to 89.9±1.5% of the assessment of the risk of formation of hypoxia.
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