Early diagnostic technique for chronic prostatitis by detecting regional microvascular endothelium dysfunction |
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IPC classes for russian patent Early diagnostic technique for chronic prostatitis by detecting regional microvascular endothelium dysfunction (RU 2540495):
 Method of prediction of meat productivity of broiler chickens / 2540435
Method enables to predict the level of body weight gain of broiler chickens on biochemical indicators of blood. This goal is achieved by calculating the lipoprotein index (LPI) in standard units by the formula:
Method for assessing disturbed haemoglobin oxygenation with reduced microviscosity of peripheral erythrocyte membrane of pregnant women in third trimester of gestation and aggravated cytomegalovirus infection / 2540425
Anti-cytomegalovirus antibody titre, microviscosity of erythrocyte membranes are measured in the peripheral blood by eximerisation of a lipid bilayer and a protein-lipid layer; the number of degenerative forms of erythrocytes, target-like erythrocytes and echinocytes, oxyhaemoglobin content are also measured. If the cytomegalovirus antibody titre is 1:1600, decreased eximerisation of the lipid bilayer to 0.59±0.006 FE/FM, of the protein-lipid layer to 0.70±0.004 FE/FM, increased number of the degenerative forms of erythrocytes to 10.5±0.3%, of the echinocytes to 8.0±0.5%, of the target-like erythrocytes to 11.5±0.9%, reduced oxyhaemoglobin to 89.8±1.5%, the risk of hypoxia is stated.
 Cartridge for automatic detection of analyte in body fluid sample and system comprising it / 2540424
Group of inventions refers to laboratory diagnostics. A device for the automatic detection of an analyte in a body fluid sample comprises an array of addressed analysis units for carrying out a chemical reaction, which generates a distinguishable signal carrying the information concerning the presence or absence of the analyte; an array of addressed reagent units referring to the individual addressed reagent unit, corresponding to the individual addressed analysis unit, and wherein the individual reagent unit is configured to be calibrated by a reference signal in the respective individual analysis unit before the arrays are assembled on the device; the individual analysis unit comprises a quantitative analysis tip having an inner surface carrying the reagents fixed on the surface for the analyte detection. The group of inventions refers to an embodiment of the above device additionally comprising a sampling unit, as well as to sub-systems comprising the above devices, methods for analysing using the above devices, a method for assembling the above devices and a method for sampling plasma from the blood sample in these systems.
 Method for assessing pulmonary blood flow / 2539718
Group of inventions refers to medicine, namely to pulmonology, cardiology, gerontology and sports medicine, and can be used for assessing the pulmonary blood flow by assessing the capillary pulmonary blood flow and intrapulmonary venoarterial bypass. That is ensured by measuring heart rate a minute, haemoglobin concentration (Hb g/l), total oxygen consumption (CO2(TOTAL) ml/min), arterial oxygen saturation (SART % or decimal portions 1) and mixed venous saturation in the greater circulation (GC) (Sv% or decimal portions 1). MBV (l/min) is calculated according to the measurements of Hb, CO2, SART, SV. Certain formulas are used to calculate the capillary pulmonary blood flow and intrapulmonary venoarterial bypass.
 Method of analysis of removal of biochemicals with meadow grass / 2538802
For analysis of removal with meadow grass of biochemicals the yield fluctuations are accounted depending on the structure of phytocenosis in the form of a ground cover. Conducting statistical data processing of testing the samples of grass from the test plots on the meander, central and terrace near flood plains, and also on the meadows with uneven and tiling location of grass species. And prior to establishment of sample plots the terrain reconnaissance is carried out with the grass cover selected for the measurements, an outline map of location of the components of the grass cover is made, then on each component of the grass cover in the form of a plot at least one temporary test plot is established, the wet and air-dry weight of the sample is determined by weighing on the cut grass sample. At that the tests on biochemical analysis are additionally carried out on the dried samples of grass to determine the concentration of at least three chemical nutrients in the form of mobile nitrogen, potassium and phosphorus oxides, and then by summing the concentrations of these three substances the total summarised removal of substances from the aerial part of grass on all test sites is calculated.
Method for assessing long-term hyperglycemia / 2538715
Invention represents a method for assessing long-term hyperglycemia based on measuring blood plasma glucose differing by the fact that glucose is measured twice: immediately after blood taking and 24 hours after blood plasma storage above a layer of blood corpuscles; that is followed by calculating a glucose reduction in percentage from the initial value which is used to assess long-term hyperglycemia with blood plasma stored at t=4.0±0.5°C.
Method for rapid analysis of cholesterol in immune complexes / 2538685
Invention can be used for the rapid analysis of cholesterol in immune complexes (CICs). Precipitated immune complexes containing multiply modified low-density lipoproteins of human blood serum are prepared by processing in buffer containing 10% PEG 3350, in ratio 1:3, incubating for 10 min at room temperature. The precipitated CICs are separated by centrifugation at 3100 g for 10 min at 23°C, diluted in PEG-free buffer; cholesterol is measured with the use of an enzymatic kit, and if the CICs content is more than 8.4 mg/dl, a high level is stated.
Method for prediction of risk of squamous metaplasia in respiratory bronchial epithelium with detected isolated basal cell hyperplasia / 2538642
Invention refers to medicine, specifically to oncology, and concerns a method for the prediction of a risk of squamous metaplasia in the respiratory bronchial epithelium with detected isolated basal cell hyperplasia. The ssubstance of the method: positive nuclear expression of the Ki67 proliferation marker and membrane differentiation expression of CD 138 squamous epitheliocytes in the bronchial epithelium are measured. If the expression level Ki67≥25%, and CD138 is ≤3.5%, the risk of metaplasia in the respiratory bronchial epithelium is predicted.
Method of predicting efficiency of neoadjuvant chemical therapy in patients with resectable basal-like triple-negative breast cancer / 2538638
Invention relates to field of medicine, namely to oncology, and can be used to predict efficiency of neoadjuvant chemical therapy (NACT) in patients with resectable basal-like triple-negative breast cancer. NACT efficiency in patients is calculated by formula: P=eY/(1+eY), where P is value of probability of sign development, Y is value of regression equation; e is mathematical constant, equal 2.72. Value of regression equation is determined by formula: Y=(42.8-1.86X1-9.3X2-2.14X3+3.19X4), where (42.8) is value of coefficient of free term regression; X1 represents subtypes of basal-like triple-negative cancer ( 1 - subgroup with expression of only CK 5/6; 2 is subgroup with expression of only EGFR1; 3 is subgroup with expression of CK 5/6 and EGFR1), (1.86) is the value of said sign regression coefficient; X2 is the level of EGFR1 expression in biopsy material (1 is high level, 2 is low level), (9.3) is the value of said sign regression coefficient; X3 is the level of expression of Ki-67 in biopsy material (1 is low level, 2 is high level), (2.14) is the value of said sign regression coefficient; X4 is chemical therapy scheme (1-FAC, 2-CAX), (3.19) is the value of said sign regression coefficient. If P<0.5, low efficiency of NACT is predicted, if P>0.5, high efficiency of NACT is predicted.
 Method for confirmation of death caused by ventricular fibrillation accompanying myocardial infarction / 2538622
For the purpose of the confirmation of a patient's death caused by ventricular fibrillation accompanying myocardial infarction in the patients died from myocardial infarction in the course of autopsy on the basis of a macroscopic pattern, the pathological process age is determined. This enables selecting examination areas in the heart at the level of necrosis. Absolute values (AV) of the following cell populations are counted in the x600-magnified visual field with using haematoxylin and eosin: necrosis-borderline lymphocytes (Lfb) in myocardial infarction of the age of 1-2 days, necrosis neutrophilic granulocytes (NGn) and fibroblasts (Fbn) in myocardial infarction of the age of 3-5 days. The derived absolute values are compared to threshold values (TV) with the threshold values of Lfb=2, NGn=38, Fbn=1. Each visual field is assigned with a diagnostic coefficient (DC), which is equal to 1.9 at the age of myocardial infarction of 1-2 days, if the absolute values Lfb<2 or -3.9, Lfb>2; if the age of myocardial infarction is 3-5 days, the diagnostic coefficient is -2.1, if AFbn<1, or the diagnostic coefficient is 7.2, if the absolute value of Fbn>1; the diagnostic value of 1.36 is shown by the absolute value of NGn<38; the diagnostic value is -11, if the absolute value is NGn>38. The total value (ΣDC) is calculated by moving between the visual fields within the examined area, and if ΣDC>12.78, the patient's death is stated to have come from ventricular fibrillation; if ΣDC<-12.78, the death is stated to be caused otherwise.
Method for predicting hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis / 2244303
One should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
 Method for predicting prostatic tuberculosis / 2244513
One should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
 Device and method for extracting nucleic acids / 2244559
The suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
Method for assay of medicinal sensitivity of tuberculosis mycobacterium / 2244927
Diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
 Endogenic pharmaceutical composition prepared on basis of goal-seeking activation of humoral mediators of brain cortex nerve ending / 2244928
Invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
 Method for estimating treatment effectiveness in atherosclerotic lower extremity ischemia patients / 2244929
Method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
 Method for predicting the result of acute peroral intoxication with cauterizing liquids / 2244930
At patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
 Method for detecting disease duration in children with chronic obstructive pyelonephritis (variants) / 2245548
Disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
 Method for predicting prostatic cancer / 2245549
The present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula:
Nutrient medium for accumulation of cell sample for following cytological and/or immunocytochemical analysis / 2246110
Invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
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FIELD: medicine. SUBSTANCE: invention refers to medicine, namely to a diagnostic technique for chronic prostatitis by detecting a regional microvascular endothelium dysfunction. Substance of the invention consists in conducting an ionophoretic assay with underlying laser doppler flowmetry, measuring a capillary blood flow reserve with an acetyl choline and sodium nitroprusside ionophoresis on the belly skin above a pubis in a projection point of the prostate, calculating an endothelial function coefficient (EFC), and if the derived EFC is less than 1.0, chronic prostatitis is diagnosed. EFFECT: using the declared technique enables simplifying the early diagnosis of chronic prostatitis by assessing the functional state of the regional microvascular endothelium. 2 ex
The invention relates to medicine, namely to urology, and can be used for the diagnosis of chronic prostatitis. The success of the practice of medicine, specifically urology and andrology, currently determined by the possibility of early detection of violations of the functional state of the microvascular endothelium. Studies of foreign and domestic authors conclusively proven that vasoregulatory endothelial dysfunction detected even before its organic damage and in the absence of clinical and instrumental signs of violation of a flow in the pelvis (C. A. Burmistrov. The prevalence of erectile dysfunction in patients with diabetes mellitus type 2. // The dissertation on competition of a scientific degree of candidate of medical Sciences. - 2004. - 23 S., I. C. Chaliapin. The value of hemorheological and hemostatic disorders in the pathogenesis of chronic prostatitis. // The dissertation on competition of a scientific degree of candidate of medical Sciences. - 2001. - 24 S.). Thus, early detection of microvascular pathology at the stage of functional disorders is an important biomedical problem. In recent years, new data on the role of violations of the functional activity of the endothelium in the pathogenesis of many urological diseases. Thus, the author of Cask is he A. L. (2009) using modern microrheological methods of research have studied the structural and functional characteristics of the skin microcirculation of the penis in humans and found its regulatory performance. For the first time the author has studied disorders of the skin microcirculation in the penis in chronic abacterial prostatitis and showed that underlying pathophysiological mechanism disorders are suppressed vasumati and structural deformation microvascular venous Department that leads to the progression of venous congestion in the tissues of the penis. It is obvious that the most feasible way to prevent the formation of irreversible vascular complications is the timely identification of functional disorders of the vascular endothelium. The interest of the vascular endothelium in the pathogenesis of vascular disease was considered from the time Virchow, however, more accurate scientific understanding of the true role of the vascular wall in the pathogenesis of many disease processes, and can check the functional state of the vascular endothelium has emerged only recently, largely as a result of the use of modern molecular biology, laboratory and instrumental methods. Existing methods for detection of endothelial dysfunction (ED) is Astelit on two areas: the detection of circulating markers of ED in serum and assessment of endothelium-dependent vasodilation. The author of Krainichenko S. C. (2009) identified key indicators of basal blood flow and the amplitude-frequency spectrum in the system microvasculature of the prostate by laser Doppler flowmetry in healthy men without diseases of the genitourinary system. Found that for healthy men characteristic sredneimportnye pulse vibrations, slow oscillations normal amplitude preserving vasomotor activity. Chronic abacterial prostatitis, complicated sexual disorders, the author establishes the presence of intravascular violations of microcirculatory, which is manifested by the decrease of peak linear, diastolic, and mean velocities in the Central and peripheral zones of the prostate gland, and decreased basal tone, increased shunting and reduced performance index of microcirculation. The author concludes that all this testifies to the intravascular disorders of microcirculation. Common drawbacks of these methods for detection of endothelial dysfunction are: - the need for invasive procedures (blood); - the inability to perform in routine clinical laboratories; - the complexity of technical performance; - the need to use expensive reaction is ivov; - the complexity of the interpretation of the obtained results. For example, elevated levels of von Willebrand factor may indicate increased activation of endothelial cells (i.e., about changing the functions of the vascular endothelium), and may be due to endothelial damage. The most reliable sign of ED is the reduction of NO synthesis, to measure the content of which is difficult due to the instability and short life span of the molecule. The NO production was estimated by the content in plasma and in urine end products of NO metabolism (nitrite, nitrate). The concentration of these substances are extremely small and have a high dependence on other sources of nitrite and nitrate, why the clinical application of this method is limited. In addition to the laboratory techniques described methods of estimation of the functional state of the vascular endothelium, based on the study of endothelium-dependent vasodilation in vivo. Thus, the known method consists in the estimation of change of the diameter of the main artery or the blood flow through it or in small resistance vessels of the body part in response to pharmacological or mechanical stimulation of the endothelium (G. I. Martsinkevich et al. Comparing the results of the functional tests used in the noninvasive assessment of endothelial function // Therapeutic archive. - 2002. No. 4. - S. 16-18. Of the pharmacological stimuli typically use the endothelium-dependent vasodilator - acetylcholine, less serotonin. Increasing the speed of the current in the main artery is achieved with the introduction of endothelium-independent vasodilators in the distal direction or mechanical stimulus by temporary occlusion of a vessel by measuring the response of large vessel on reactive hyperemia (A. A. Popov et al. Evaluation of vasomotor function of vascular endothelial dysfunction in young patients with arterial hypertension // Russian family doctor. - 2006. No. 1. - S. 31-32). Incentives studied during direct angiographic (mostly coronary) rendering. However, this method has significant drawbacks: - the need for appropriate equipment for diagnostic procedures and conditions for conducting research; - the complexity of technical performance; - the need for special facilities and trained personnel; - invasiveness and high cost koronaroangiografiulad research; - the inability to perform koronaroangiografii in the dynamics (for monitoring changes of endothelial function under the influence of the treatment). So become more and more widespread non-invasive methods. In 1992, D. Celermajer et al. developed a non-invasive Ultrazvuk the Oh Doppler method for determining the functional state of the endothelium of peripheral vessels, uses mechanical stimulation of blood flow synthesis and excretion endothelium relaxing factor produced. Increase blood flow was achieved by temporary occlusion of the brachial artery pneumatic cuff pressure above systolic 50 mm RT.article then suddenly her "deflation". In the works of D. Celermajer et al. the location of the artery was performed above the place of occlusion (D. S Celermajer. Noninvasive detection of endothelial dysfunction in children and adults at risk of atherosclerosis // Lancet. - 1992. - Vol.340, No. 8828. - P. 1111-1115). At the same time in the Department of new methods of research and diagnostics Russian cardiology research industrial complex Ministry of health in conducting this research uses the classic breakout with reactive hyperemia, in which the artery scan below the occlusion. According to D. A. Staikova and B. A. Sidorenko, this methodology allows to identify the endothelial dysfunction in patients with arterial hypertension and ischemic heart disease (B. A. Sidorenko, D. A. Staikov. Endothelial dysfunction in the pathogenesis of atherosclerosis and its complications / // Kremlin medicine. Clinical journal. - 1999. No. 2. - S. 7-12). Currently have the opportunity to assess the state of the vascular endothelial function using laser Doppler flowmetry (LDF), based on optical sensing of tissues helium-neon laser (Century. And. To the units et al. The method of laser Doppler flowmetry: a Manual for physicians. - M., 2000. - 35 C.). The advantage of this method is its non invasiveness, the possibility of long-term monitoring, the absence of contraindications. To assess vasomotor endothelial function are ionophoretically samples with the introduction of endothelium-independent and endothelium-dependent vasodilators (I.e. Konyayeva et al. Conditions ionophoretically samples with acetylcholine and nitroprusside for assessment of microvascular endothelium of human skin // Bulletin of new medical technologies. - 2004. - T. XI, No. 1-2. - S. 68-70). The closest to our proposed method is the diagnosis of endothelial dysfunction in patients with bronchial asthma using laser Doppler flowmetry with a calculation of the coefficient of endothelial function (EFC) by the ratio of the allowance of capillary blood flow in the sample with acetylcholine to the provision of capillary blood flow in the sample with sodium nitroprusside (I. C. Nurzhanova et al. Pat. 2436091 OF THE RUSSIAN FEDERATION, IPC G01N 33/483. The method of assessing the functional state of the microvascular endothelium in patients with bronchial asthma / (RF, GOU VPO ASMA University). No. 2010124218/15 Announced. 11.06.10; Publ. 10.12.11. Bull. "Of the invention. Utility models", No. 34). The disadvantages of this method are: - described practical application only in pain who's bronchial asthma; - evaluation of the functional state of the vascular bed as a whole (i.e., detection of disorders systemic) without detail and without taking into account regional blood flow, in particular blood flow in the prostate gland. The invention is aimed at facilitating the early diagnosis of chronic prostatitis by evaluating the functional status of regional microvascular endothelium. This technical result is achieved by measuring the performance reserve capillary blood flow during iontophoresis of acetylcholine and sodium nitroprusside on the skin of the abdomen above the fold at the point of projection of the prostate, calculate the ratio of endothelial function (EFC) and the EFC value below 1.0 diagnosed with chronic prostatitis. For the clinical assessment of vasomotor endothelial function during LDF testing we conducted pharmacological tests using specific agents causing endothelium-independent (ENVD) and endothelium-dependent (EDVD) vasodilatation. Namely, using local iontophoresis we carried out sequential introduction of two solutions: the endothelium-independent vasodilator - sodium nitroprusside (5%) and the endothelium-dependent vasodilator, acetylcholine was used (5%). The study was conducted at the point of projection of prestate Inoi glands on the skin of the abdomen above the fold. The choice of this region is due to the fact that it reflects the blood flow in the prostate gland. During each ionophoretically samples in addition to temporal parameters, we estimated RCM-reserve capillary blood flow (degree of growth of the microcirculation in response to iontophoresis of the drug). As is known, the estimate of the degree of endothelial dysfunction based on a comparison of vascular response in response to the introduction of endothelium-dependent and endothelium-independent vasodilators. The response to sodium nitroprusside (NN) reflects the relaxation of blood vessels, caused by the direct action of nitric oxide on smooth muscles. The acetylcholine (ach) is used to demonstrate vasodilatation, mediated by endothelium that allows you to use this test to assess the functional state of the endothelium (specifically the ability to synthesize the factors relaxation). Endothelial dysfunction is manifested in the reduction of the severity of the reaction to acetylcholine: the maximum value of the index of microcirculation (PM) in this case is less than in the case of response to sodium nitroprusside, and in proportion to the degree of dysfunction. To detect dysfunction of regional microvascular endothelium by undertaking a comparative analysis of endothelium-dependent and endothelium-independent vasodilation, according to the recommendations of the Institute. Organovo et al., we calculated the ratio of endothelial function - the EFC as the ratio of the RCM AH / ALAC NN (%) [degree of growth of the microcirculation during the iontophoresis of acetylcholine to the degree of increase in GR, during iontophoresis of sodium nitroprusside]. According to the LDF testing at the point of projection of the prostate gland on the skin of the abdomen above the fold of the patients in the control group (i.e., men without prostate) is the EFC was 1,08±0,06% EFC Is greater than 1.0 indicates the absence of regional dysfunction of the endothelium: the response of the vascular bed (in the form of growth of microcirculation) on endothelium-dependent stimulation prevail over those of the endothelium-independent stimulation. In the control group, the presence of prostatitis were excluded according to common urological procedures: complaints, and rectal ultrasound of the prostate, smear discharge from the urethra, microscopy of prostate secretion. Men suffering from prostatitis, LDF-testing the skin of the abdomen above the fold at the point of projection of the prostate was diagnosed dysfunction of regional microvascular endothelium, namely, the value of the EFC is less than 1.0. While 36% of patients did not show any specific complaints, the diagnosis of "Chronic prostatitis" was exhibited for the first time according to further examination almarine is passed urological techniques. Another interesting fact that with the proven diagnosis of "Chronic prostatitis" and the value of the EFC at the point of projection of the prostate is less than 1.0, the ratio, calculated at the point characterizing the systemic circulation (on the skin of the left forearm in the midline at 4 cm above the base of the styloid processes of the ulnar and radial bones), in 62% of patients exceeds 1.0. Thus was established the relationship: dysfunction of regional microvascular endothelium (i.e., the EFC at the point of projection of the prostate less than 1.0) - the presence of chronic prostatitis. The study was carried out calculation of diagnostic sensitivity (DF) method by the formula: QH=a/(a+C), where a is the number of matches on the presence of the disease (true positive results); - the number of false-negative results (β-error). Also calculated diagnostic specificity (DS) method by the formula: DS=d/(d+b), where d is the coincidence conclusions about the absence of disease (true negative); b - false-positive result (α-error). Was calculated diagnostic efficiency (DE) of the method according to the formula TE=(DF+DS)/2 is the average between DF and DS. Was calculated also: - predictive value of a positive result (PZPR) according to the formula: PCP=a/(a+b) is the probability of the presence of the disease with a positive test result. - prognostic valuable the th negative result (PCOR) according to the formula: PCOR=C/(C+d) - the probability of the absence of the disease with a negative test result. Thus, the diagnostic sensitivity of the test was 96%, and its diagnostic specificity of 95%. The overall diagnostic efficacy of the test was also relatively high with 95.5%, and predictive value of a positive result is 93%. All this allows to recommend this method for the diagnosis of chronic prostatitis. A positive result makes it likely to assume the examined patient of chronic prostatitis. We offer to evaluate the functional status of regional microvascular endothelium by LDF-test on the skin of the abdomen above the fold at the point of projection of the prostate with the calculation of the EFC expands the Arsenal of diagnostic tools and facilitates early detection of chronic prostatitis. This method was successfully tested on the clinical basis of the Department of urology and Nephrology state budgetary educational institution of higher professional education "Astrakhan state medical Academy" of the Ministry of health of Russia - in the urology Department and clinic of the establishment of health care JSC Alexander-Mariinsky regional clinical hospital, also in terms of outpatient departments the establishment of health care JSC "Regional dermatovenerologic dispensary, Astrakhan in 2011-2012 Surveyed 226 men Astrakhan region, of whom 97 Dunn is m LDF-test was diagnosed dysfunction of regional microvascular endothelium (EFC< 1) and was suspected chronic prostatitis, later confirmed by clinical and ultrasound examination by 90 people. Among 129 patients with normal regional microvascular endothelial chronic prostatitis was diagnosed in only 4 people, and 125 patients this diagnosis was excluded, which allowed to form from among the control group. The age of examined patients was - 34 [22; 43]. The median duration of disease was 12 [2; 34] years. Persons in the control group were comparable in age with the examined patients with chronic prostatitis. Below are the results of testing. In the group of men without prostate, recognized control were conducted by conventional methods of diagnosis of prostatitis, and advanced LDF was carried out testing of regional dermal microvessels in the coupe with ionophoretically samples. The value of the EFC on the skin of the abdomen above the fold in men with prostatitis, made up 1.08±0,06% Example 1 Patient K., 37 years (medical outpatient map No. 2124), appealed to the Regional dermatovenerologic dispensary 08.08.2011. Complained of pain in the perineum, inguinal region, erection and orgasm, decreased libido, frequent urge for urination, pollakiuria, sluggish stream of urine. Marked is l also fatigue, weakness, irritability. Ill for about 7 years. The beginning of the disease was burning in the urethra, which appeared after hypothermia. Was diagnosed with Chronic bacterial prostatitis. About this diagnosis are often treated on an outpatient basis, receiving in each case, antibacterial drugs, massage of the prostate, physiotherapy. Objectively. Build giperstenichesky. From internal organs pathology it is not revealed. Adult male of a male. External genitals are developed correctly. Rectal examination. The prostate gland is enlarged, swollen, smooth, painful, smooth midlevel furrow. Ultrasound examination. Kidney is normal in size, contours smooth. Cup-pelvis system is not changed, pathological processes it is not revealed. The prostate gland is visualized in size of 4.8×5,5×5 cm, with fuzzy and jagged contours. After voiding residual urine volume 30 ml. Marked diffuse decrease in echogenicity of the prostate gland, dilatation paraproctitis venous plexus, cystic inclusions. After provocation gonococcal vaccine (under the scheme) held smear discharge from the urethra, as well as the microscopy of prostate secretion. Detected leukocytes 11-15-22 in the field of view. Les is siminovich beans a little. Protein content in prostate secretion were: LF-157 mg/l, FSC-3315 mg/l, DOG-2221 mg/L. Wasserman negative. Analysis of HIV - negative result. Microscopic examination of smears from prostate secretion in the color of Gram-detected gram-positive cocci: diplococci, streptococci, cocci in clusters; on the enzymatic activity were determined following types of staphylococci: S. aureus, S. epidermidis, Str. agalactiae, Str. faecalis., E. coli. In General, the analysis of blood (09.08.11): erythrocytes - 4,63×1012/l, hemoglobin - 129 g/l, leucocytes - 5,9×109/l; eosinophils - 2%, stab neutrophils - 2%, segmented neutrophils - 63%, lymphocytes 25%, monocytes - 4%; ESR - 23 mm/hour. Urinalysis (09.08.11) no pathological features. ECG (08.08.11): sinus tachycardia, electrical axis of the heart is not rejected. Exhibited clinical diagnosis of Chronic bacterial prostatitis in the acute phase. According to LDF-test ionophoretically samples when applying the ratio of endothelial function (EFC) on the skin of the abdomen above the fold at the point of projection of the prostate, the patient was 0,79 (ALAC AH=169,6%, ALAC, NN=214,7%). Additionally was calculated EFC at LCF testing at the point characterizing the systemic microcirculation (on the skin of the left forearm in the midline at 4 cm above the base of the styloid processes of the elbow and the beam is howling bones), amounting to 1.1. Thus, was diagnosed dysfunction of regional microvascular endothelium at the point of projection of the prostate on the skin of the abdomen above the fold. Example # 2 Patient K., 35 years (medical outpatient map No. 1139), turned to the clinic and was admitted to the urology Department of the establishment of health care JSC Alexander-Mariinsky regional clinical hospital" , Astrakhan 15.04.2012. Complained of pain in the perineum, scrotum, inguinal region, erection, decreased libido. Also noted fatigue, weakness, irritability, poor sleep. Sick for about 9 years. Repeatedly outpatients receiving in each case, the massage of the prostate, physiotherapy, physiotherapy and anti-inflammatory drugs. The effect of therapy was short, so repeatedly observed recurrences of the disease. From the anamnesis of life: the patient leads a sedentary lifestyle, sex single in the course of the year. Objectively. Build giperstenichesky. From internal organs pathology it is not revealed. Adult male of a male. External genitals are developed correctly. Rectal examination. The prostate gland is enlarged, swollen, painful, smooth midlevel furrow. Ultrasound is the surveys. Kidney is normal in size, contours smooth. Cup-pelvis system is not changed, pathological processes it is not revealed. The prostate gland is visualized in size of 6.8×6,5×7 cm, with fuzzy and jagged contours. After voiding residual urine volume 20 ml. Microscopic examination of smears from prostate secretion in the color of Gram-detected isolated gram-positive cocci: diplococci, cocci. In General, the analysis of blood (16.04.12): erythrocytes - 4,63×1012/l, hemoglobin - 129 g/l, leucocytes - 4,9×109/l; eosinophils - 6%, stab neutrophils - 2%, segmented neutrophils - 63%, lymphocytes 25%, monocytes - 4%; ESR - 15 mm/hour. Urinalysis (16.04.12) no pathological features. ECG (15.04.12): sinus rhythm, electrical axis of the heart is not rejected. After provocation gonococcal vaccine (under the scheme) held smear discharge from the urethra, as well as the microscopy of prostate secretion. Detected leukocytes 1-2-4 in the field of view. Lacitignola grains are rare. The Wasserman negative. Analysis of HIV - negative result. Based on the above data was put the final diagnosis: Chronic congestive prostatitis in the acute phase. The patient was treated by standard baseline therapy. According to LDF-test ionophoretically samples the ri receiving the coefficient of endothelial function (EFC) on the skin of the abdomen above the fold at the point of projection of the prostate in patients was 0.69 (ALAC AH=118,1%, ALAC NN=171%). Additionally was calculated EFC at LCF testing at the point characterizing the systemic microcirculation (on the skin of the left forearm in the midline at 4 cm above the base of the styloid processes of the ulnar and radial bones), amounting to 0,9. The specified value of the EFC at the point of projection of the prostate confirms the presence of dysfunction of regional microvascular endothelium in patients with chronic prostatitis. The proposed way of detecting dysfunction of regional microvascular endothelium is achieved simplification of early diagnosis of chronic prostatitis. To evaluate the functional status of the microvascular endothelium in the level of the EFC allows to reliably determine the presence of endothelial dysfunction. The method of laser Doppler flowmetry meets the requirements of modern, non-invasive, has no contraindications, simple in execution and can be used for dynamic monitoring. When production samples are not used expensive or toxic reagents. In addition, the important point is the fact that the results of laser Doppler test can be mapped to common in urological practice methods. The method of early diagnosis of chronic prostatitis by identifying dysfunction regional microvascular endothelium by holding ionophoretic the definition of the sample during laser Doppler flowmetry, characterized in that the measuring indicators of reserve capillary blood flow during iontophoresis of acetylcholine and sodium nitroprusside on the skin of the abdomen above the fold at the point of projection of the prostate, calculate the ratio of endothelial function (EFC) and the EFC value below 1.0 diagnosed with chronic prostatitis.
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