Diagnostic technique for adolescent's reproductive disorders accompanying chronic renal pathology |
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IPC classes for russian patent Diagnostic technique for adolescent's reproductive disorders accompanying chronic renal pathology (RU 2536288):
Method of diagnostics of menstrual dysfunction against background of polycystic ovarian syndrome by immature girls / 2535096
For immature girls after day 3-5 of menstrual cycle a level of Anti-Mullerian Hormone is determined in blood serum by method of enzyme multiplied immunoassay (ELISA), and if its value exceeds 5.2 ng/ml the menstrual dysfunction is diagnosed against the background of polycystic ovarian syndrome.
Method for early prevention of gestational complications / 2530624
Method involves detecting a threatening miscarriage and a carrier state of the polymorphism in the gene of folate metabolism, as well as a CD54+ lymphocyte ratio, lactoferrin and β-subunit of human chorionic gonadotropin (β-HCG) levels in venous blood of a pregnant woman from the onset of pregnancy to the end of the first trimester. That is followed by calculating a prognostic index (PI) by formula: PI=0.7199X1+1.2552X2-0.00653X3-0.0009X4+0.0722X5+1.1277, wherein X1 is the threatening miscarriage, yes/no (1/0); X2 is the polymorphism in the gene of folate metabolism, yes/no (1/0); X3 is the CD54+ lymphocyte ratio, %; X4 is the lactoferrin level, ng/ml; X5 is the concentration of the free β-subunit of human chorionic gonadotropin (β-HCG), ng/ml. If PI<0, the gestational complications are predicted, while PI>0 enables stating a low risk of pathological conditions accompanying pregnancy. A sensitivity of the presented method makes 81.2%, its specificity is 85.1%. The method effectiveness is 83.2%.
Diagnostic technique for renal functional reserve / 2528903
Invention refers to medicine. Substance of the early diagnostic technique for chronic renal disease consists in using a dosage range of dopamine of 1 to 3 mcg/kg of body weight and a standard water load of 200 ml. No glomerular filtration rate increase testifies to the presence of early signs of chronic renal disease.
Diagnostic technique for clinically latent hypercorticoidism in patients suffering from stype 2 diabetes mellitus or obesity / 2521387
First stage comprises a night suppressive test with dexamethasone 1 mg with a test considered to be positive, if plasma cortisol measured at 8.00 in the next morning exceeds 50 nmole/l. If the first stage has a positive result, the second stage is performed 1-2 days later. At the second stage, blood plasma cortisol at 24.00, daily urine free cortisol, a coefficient of circadian rhythm of cortisol secretion are determined on the same day. If at least two of the three test results are above normal: plasma cortisol at 24.00 is more than 207 nmole/l, daily urine free cortisol is more than 180 mcg/day, coefficient of circadian rhythm of cortisol secretion is more than 50%, hypercorticoidism syndrome is diagnosed. The presented technique provides higher accuracy and simplifies diagnosing of the given disease.
 Solid phase enzyme-immunoassay (eliza) for vascular endothelial growth factor (vegf) / 2517301
Invention relates to the field of immunology, namely to enzyme-immunoassay, in particular to a method of detecting forms of vascular endothelial growth factor (VEGF) with a size more than 110 amino acids in a biological sample. The method includes the following stages: contact and incubation of the biological sample with an uptake reagent, immobilised on a solid substrate, where the uptake reagent contains a monoclonal antibody, which recognises and specifically binds with residues, in quantity more than 110, from human VEGF; separation of the biological sample from the immobilised uptake reagents; contact of the immobilised molecular complex of the reagent of the uptake-target with detected antibody, which binds with VEGF domains, responsible for binding with KDR and/or FLT1 receptor, or which binds with an epitope in VEGF1-110; measurement of the level of VEGF110+, bound with reagents of the uptake, with application of means of detection for the detected antibody. Set of immune assay reagents for detection of VEGF110+ forms in the biological sample. An antibody 5C3, obtained from hybridoma 5C3.1.1 with a depositary number PTA-7737, with the said antibody 5C3 binding VEGF110+ forms, including VEGF121+. Hybridoma 5C3.1.1, deposited in ATCC with the depositary number PTA-7737, to obtain the monoclonal antibody 5C3.
Method for determining degree of severity of chronic obstructive pulmonary disease / 2516971
Blood is examined. The steroid hormones cortisol (nmole/l) and DHEA-S (mcmole/l), as well as the oxidative stress values - oxidative modified proteins (OMP, nM/mg, protein), malondialdehyde (MDA, nM/ml) and SH groups mg% are evaluated. The forced expiratory volume 1(%) FEV1 is calculated by formula on the basis of the derived values. If the derived FEV1 is within 50% to 80%, the presence of a moderate degree of chronic obstructive pulmonary disease; the FEV1 being within the range of 30 to 49% means a severe degree of chronic obstructive pulmonary disease (COPD), and the derived value being less than 30% shows an extremely severe degree of COPD.
Method for prediction of risk of early development of atherosclerosis in patients with chronic prostatitis / 2504782
Blood serum of the younger patient suffering chronic prostatitis is examined for total testosterone, sex hormone-binding globulin to calculate a free testosterone index; high-density lipoproteins and triacylglycerides are determined, and an atherogenic index is calculated by formula. If the atherogenic index is <3.7, a high risk of the early development of atherosclerosis is predicted.
 Method for prediction of recurrent non-small-cell lung cancer / 2498305
What is involves is the histological examination of tissue fragments taken from the extracted lung with primary, intermediate and segmental bronchi at 4-5 cm from the tumour, and disregeneration change cases are determined in the respiratory bronchial epithelium, including: basal cell hyperplasia (BCH), squamous metaplasia (SM) and if observing a combination of basal cell hyperplasia and squamous metaplasia (BCH+SM+) in the respiratory bronchial epithelium adjacent to the tumour, a risk of developing the recurrent non-small-cell lung cancer is predicted.
 Method for prediction of recurrent cervical cancer / 2485523
Method for prediction of recurrent cervical cancer involves biochemical daily urine analysis to determine daily urine androsterone and etiocholanolone to be related; if the relation is 0.75 mg/day or less, the recurrent disease is predicted for the first 2 years, and if the relation exceeding 0.75 mg/day, a prolonged recurrence-free period up to 10 years or more is predicted.
 Method for aromatase activity test / 2481587
Blood serum is examined for lutropin, follitropin, oestradiol, total testosterone and free testosterone levels before and 48 hours after the oral administration of 450 aromatase inhibitor, letrozole 10 mg, and the variations of lutropin and follitropin, as well as of the oestradiol/total testosterone and oestradiol/free testosterone relations are used to asses aromatase activity in points assigned by a certain way to consider low aromatase activity shown by total score 0 to 7 points, normal aromatase activity - 8 to 14 points, and high aromatase activity - 15 points or more.
 Method for assessing severity and possible outcome of retinopathy in premature newborn children / 2536284
At the first stage of a subtotal vitrectomy, the vitreous body is sampled with its biochemical parameters to be analysed. In an air-free environment, the vitreous body samples are analysed to determine the following values at 37°C by means of an automatic blood gas and acid-base balance analyser: pH, partial carbon dioxide pressure pCO2, actual bicarbonate ion
Instant diagnostic technique for acute intestinal infections / 2536243
Invention represents an instant diagnostic technique for acute intestinal infections (AIIs), involving detecting indication markers of the AII aetiology with the use of laboratory immunology tests, differing by the fact that the AII aetiology is stated in children of an early age category, preferentially in the newborn children; that is accompanied by measuring the concentration of cytokine, interleukin IL-10 in coprofiltrate and diagnosing chronic placental insufficiency (CPI); a probability (P) of the bacterial AII aetiology is calculated; the value P of more than 50% testifies to the bacterial AII aetiology, while the value P being less than 50% shows the absence of the bacterial AII aetiology, and enables considering the diagnostics second stage to be necessary, which implies measuring the concentration of cytokine, interleukin IL-4 in coprofiltrate; the time of latching the newborn child to the breast is established with considering the type of feeding; that is combined with calculating a probability (P) of the viral or viral-bacterial AII aetiology, with the value P of more than 50% testifying to the viral AII aetiology, while the value being less than 50% makes it possible to state the viral-bacterial AII aetiology.
 Method for assessing inducing action of aggravated cytomegalovirus infection in pregnant woman in third trimester of gestation on methaemoglobin and haemoglobin oxygenation in newborn's peripheral blood / 2536235
Assessing the inducing action of cytomegalovirus infection on a newborn's erythrocyte methaemoglobin and oxyhaemoglobin in peripheral blood in aggravated cytomegalovirus infection in a pregnant woman in the third trimester of gestation is ensured by determining erythrocyte count pre-stained with 0.1% methylene blue. The cytomegalovirus antibody titre being 1:1600 accompanied by an increase of the erythrocyte count 45 min after the staining to 62.5±1.9%, an increase of methaemoglobin to 1.8±0.08%, a decrease of oxihaemoglobin to 96.5±2.9% is stated as a disturbed methaemoglobin recovery and reduced haemoglobin oxygenation that leads to developing haemic anaemia in a newborn.
Method for prediction of stage of multiple sclerosis taking into account biochemical status values / 2535447
Invention refers to medicine, namely to neurology, can be used to specify a stage of aggravation or remission for the patients suffering multiple sclerosis (MS). The method is based on using the biochemical status values normally measured in the patient. A regression equation is used to determine the stage of multiple sclerosis: the stage of the clinical course of MS=2.5235 - 0.914006*sex+0.00355359*age+0.0702341*glucose+0.0131744*aspartate transaminase - 0.00412952*alanine transaminase - 0.0484807*bilirubin+0,153119*thymol test+0.196675*carbamide+0.00223117*cholesterol - 0.207918*amylase -0.00755805*total protein, wherein females are indicated as 0, females - as 1; the age is expressed in completed years at the moment of examination; glucose, alanine transaminase, bilirubin, thymol test, carbamide, cholesterol are absolute values measured in venous blood. If the derived value falls within the range of 2.89-3.48, the patient is stated to have remitted MS, while the value exceeding 3.48 enables diagnosing aggravated MS.
Method for identifying recessive factors of genetic predisposition to breast cancer / 2535157
Invention represents a method for identifying the factors of genetic predisposition to breast cancer involving genetic marker recording by a massive parallel sequencing followed by sampling and analyzing in depth injurious mutations only among the patients suffering breast cancer; a differential characteristic of the method is that identifying the recessive factors of the genetic predisposition to breast cancer enables involving the patients with a medical history having an early age of the onset, primary multiple cancer and no cases of breast cancer or ovarian cancer in the family; the analysis procedure covers only the mutations found in the homozygous state.
Method of diagnostics of menstrual dysfunction against background of polycystic ovarian syndrome by immature girls / 2535096
For immature girls after day 3-5 of menstrual cycle a level of Anti-Mullerian Hormone is determined in blood serum by method of enzyme multiplied immunoassay (ELISA), and if its value exceeds 5.2 ng/ml the menstrual dysfunction is diagnosed against the background of polycystic ovarian syndrome.
 Method for liposuction injury evaluation / 2535091
Lipoaspirate is collected into a container; a liquid portion of the lipoaspirate is sampled from the bottom of the container and diluted in normal saline; that is followed by red blood count in the Goryaev's chamber in five large squares, each of which consists of 16 small squares by formula:
Method for prediction of clinical course of atrophic anaemia following splenectomy / 2535059
Splenectomy is followed by determining its weight, carrying out morphometric measurements of an average area of the white pulp of the lymphoid organ in histological sections stained by haematoxylin and eosin. These values are taken into account to calculate a mass of the white pulp by formula: m=mspleen×D%/100%, wherein m is the mass of the white pulp (g), mspleen is the total mass of the spleen (g), D is the average area of the white pulp, %. If the calculated value is ≤8.4 g, the favourable prognosis for atrophic anaemia is established, and if the value is >8.4 g, the unfavourable prognosis of the disease is stated.
 Methods and sets for predicting risk of infusion responses and antibody-mediated loss of response in therapy with pegylated uricase by monitoring uric acid content in blood serum / 2535038
Group of inventions relates to medicine, namely to therapeutic treatment of gout and prevention of accompanying side effects and complications in patients. method of prevention and prediction of risk of infusion responses in case of intravenous introduction of pegylated uricase to patients with gout consists in determination of uric acid content in patient's blood serum with the following indication to cessation of therapy at the level of approximately 4 mg/dl. Claimed method also makes it possible to identify risk group of patients, who receive pegylated uricase, with reduction of treatment efficiency due to formation of antibodies and higher risk of infusion response development.
Method for assessing activity of inflammatory process / 2535026
Venous blood samples are examined to measure rod nuclear cells according to clinical blood analysis findings, C-reactive protein according to biochemical blood assay findings, fibrinogen according to coagulologic analysis findings; tracheal aspirate samples are examined to measure neutrophil leukocytes according to cytologic analysis findings, isolated gram-positive and gram-negative clinical strains of microorganisms or a combination thereof and a quantity of a rate of growth of the microbial biomass. The prepared blood and tracheal aspirate values are analysed and assessed according to the 3-point scale. The total score is calculated, and the derived value is used to assess the inflammatory process activity: 6-9 points - low: 10-14 - medium; 15-18 - high.
Method for predicting hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis / 2244303
One should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
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FIELD: medicine. SUBSTANCE: technique involves a blood serum examination for thyrotropic hormone, mcUnit/ml, free T4, pmole/l, prolactine, mIU/ml, follicle-stimulating hormone, mIU/ml, lutenizing hormone, mIU/ml, testosterone, nmole/l, dehydroepiandrosterone sulphate, mcg/ml, cortisol, nmole/l, oestradiol, pg/ml, 17-OH progesterone, nmole/l. Calculating an integral hormone state (HIS) by formula follows. If the HIS value is below 0.799, a habitual disorder is stated, which is accompanied by a minimum risk of the reproductive disorders. The HIS values falling from the range of 0.800 to 1.000 provides stating the tensed hormonal regulation reflecting a moderate risk of the reproductive disorders. The disturbed interhormonal cooperation corresponds to the HIS value falling within the range of 1.001 to ≤1.210 that represents a high risk of the reproductive disorders. The HIS value of more than 1.211 shows the lack of reserves that testifies to a very high risk of the reproductive disorders. EFFECT: technique enables improving the diagnosis of the adolescent's reproductive disorders. 5 tbl, 3 ex
The invention relates to medicine, namely to Pediatrics, Nephrology, endocrinology, and can be used to predict reproductive disorders in adolescents by the value of the integral indicator of hormonal status (IPGS). Physical and sexual development of adolescents influenced quite a number of factors, including the presence of chronic pathology of the kidneys, impairing the formation of the reproductive system in the period of puberty, which subsequently may lead to sterility, miscarriage and other (Perepelkina N. Y. Ways to improve pediatric Nephrology service: Diss.Dr. med. - Moscow, 2003. - 372 S.). It is known that disturbances in reproductive health lead to changes in hormone levels (Zhukovsky, M. A. pediatric endocrinology. - M.: Medicine, 1995. - 656 C.). Reserve improve reproductive health is early diagnosis of his deviations. There are a predictor of reproductive health through screening assessment of reproductive health (RU # 2271152 C1, 26.05.2008, RU # 2393757 C1, 25.05.2009), which consists in determining the score of the indicator occupational factors, additionally determine medical history, medical and biological proxy indicators and calculate the diagnostic indicators of risk formula is m The disadvantages of this method are restrictions on profession, age and gender, as well as a lack of objectivity due to the lack of evaluation of hormonal profile. The closest analogue is the way to determine a disturbance of the hormonal status in women (RU # 2225009 C2, 13.05.2002), which recommended that the identification konstitucionno-phenotypic characteristics and obstetric history, highlighting the stages of disorders of hormonal status. The disadvantages of the prototype are: - staging disorders of hormonal status on clinical manifestations that adolescents may be missing; - lack of comprehensive changes in the level of hormones that are involved in the formation of reproductive function; - not defined differentiated border of the complex indices of hormones in the comprehensive assessment of reproductive health on the psycho-physiological level. The objective of the invention: improved diagnostics, simplifying the interpretation of the data and improving the accuracy of prenosological diagnosis of reproductive health disorders in adolescents. The technical result - the possibility of early preclinical diagnosis of reproductive health disorders in adolescents with chronic kidney pathology by identifying the Oia rules and violations of the hormonal status. Positive effect: method allows to diagnose reproductive health in adolescents at the pre-clinical stage, which gives the opportunity to define the tactics of treatment and preventive measures in the early stages of the disease. The technical result is achieved by the fact that in the serum in the first phase of the cycle enzyme-linked immunosorbent assay (ELISA) define sexual, thyroid hormones, and adrenal hormones, reflecting the reproductive function in adolescents (Levenets S. A. Clinico-anamnestic and hormonal features of oligomenorrhea and secondary amenorrhea in adolescent girls with systemic connective tissue dysplasia / S. A. Levenets, L. F., Kulikova, T. A. Nachalova // Obstetrics and gynecology. - 2006. No. 1. - S. 39-41). Then the integral indicator is calculated hormonal status (IPGS) according to the formula where Xi is the value of the index in a particular patient, X - average, healthy; /Xi-X/ - difference between indices without regard to sign, σ is the standard deviation of the index in the healthy group; n is the number of parameters. On the basis of the received results of the analysis of the gradation indices and the calculated ranges of values of the function IPGS. Using cluster analysis (method k-means) allocated 4 cluster with different what ipathname gradations integral index: 1 cluster: in this group teamed Teens who are in 92.5% of cases there were no violations of reproductive health, which allowed to determine the indicators of hormonal status in adolescents as usual reject" indicator IPGS in this group to 0,799, when there is minimal risk of the presence of abnormalities in reproductive health. Adolescents 2 cluster reproductive health identified in 13.5% of cases, which allowed to determine the indicators of hormonal status in adolescents as "tension processes hormonal regulation" and IPGS ranged from 0.800 to to 0,999 that reflects the average risk of variance in reproductive health. 3 cluster teamed Teens who are in 31% of cases of reproductive health conditions that require observation and correction. Adolescents in this group IPGS corresponds to the values from 1,000 to 1,210 (violation rigormarolee cooperation) and indicates a high risk of development of reproductive health disorders. 4 cluster amounted to Teens who are in 48% of cases defined diseases of the reproductive those in need of treatment and control. IPGS adolescents were equal to or above 1,210 (lack of reserve capacity). This figure indicates a very high risk of the presence of abnormalities in the reproductive the health of adolescents with HSP. For disclosure of the invention the results of the research 139 adolescents with chronic renal pathology for the diagnosis of reproductive health disorders and indicators of hormonal status. According to the study Protocol were compared reproductive health and hormonal status of adolescents. Indicators of hormonal profile at 39.8 per cent of girls and 41,3% of boys fit into the usual deviation of the level of hormones (table.1). The fifth of the children (18.3% of girls and 21.7% of boys) was determined by the lack of reserve capacity. With the growth of IPGS increases the frequency variance in reproductive health (table.2): if in a group of children with "normal abnormalities" disorders of the reproductive health identified in 7.5% of adolescents in the group "the lack of spare capacity" - 48%. Using the proposed method for determining IPGS characterizing changes in hormonal status, can identify children with functional tension body systems for early diagnosis the diagnosis of abnormalities in the reproductive health status in a particular teenager, and promptly appoint treatment and preventive measures. As a result of processing of the analytical data, it was found that the hormonal status is a fairly sensitive indicator of reproductive is about and General health of the adolescent. On the basis of the data obtained with the prospect of timely detection of irregularities in reproductive health or predisposition thereto and is IPGS possible to make the plan of examination and treatment of adolescents with chronic kidney disease. The advantages of the proposed method: 1. changes in hormonal status ahead of the clinical picture, or other data other diagnostic methods; 2. integral assessment of hormonal status simultaneously on a number of indicators for the most complete diagnosis of reproductive health disorders in adolescents; 3. the ability to highlight the most significant for the health disorders of hormones from a population of subjects. Example 1. The patient Alexey P., 15 years. The history of the disease at admission: 1 year 7 months. surveyed in the urological Department of the regional hospital where diagnosed with only the right kidney. In 3 years received treatment in the hospital about exacerbation of chronic pyelonephritis; in 11 years - again exacerbation of the disease. After discharge observed outpatient for 4 years in urine - without a pathology. Calculated IPGS the child:
IPGS the child - 0,21753. The teenager is a group of "usual deviations". Inspection specialists have not revealed disturbances in reproductive health. Example 2. The patient, Mary F., age 14. The history of the disease: changes in urine for the first time identified the child in 2002 in the form of erythrocyturia 10-12 in p/C, Microlaboratory. Further in the analysis of urine was noted microhematuria. The girl was surveyed in 2010, where first diagnosed: tubulointerstitial chronic nephritis, toxic-allergic Genesis, the active phase. Complaints on admission to changes in the urine, edema of the eyelids in the morning, infrequent urination; headache mainly in the eternal is rnee time of day. The deviation of the organs and systems: the skin is clean, dry, pale, periorbital shadows. Body weight 45 kg; length 167 cm; OGK 66 cm; ORD 53 see Physical development metasomatically disharmonious. Sexual formula: mA inputs3, P3, Ah, Me. The calculation of IPGS the child:
IPGS a teenager 1,19768 that corresponds to the violation rigormarolee cooperatives due to the low level of estradiol, which the girl significantly lower compared with the control group, and with the established rules. The girl examined by experts, who recovered from the immaturity of secondary sexual characteristics. Ultrasound examination of small pelvis (uterus, ovaries) pathology it is not revealed. Example 3. The patient Xenia E., 15 years. The history of the disease at admission: child in 4 months. was first diagnosed with pyelonephritis. After conducting a comprehensive survey diagnosed Congenital malformations of urinary system: iliac dystopia, hypoplasia, a doubling of the right kidney. Bilateral VUR grade 2-3 (condition after endoscopic correction of VUR right). Secondary obstructive pyelonephritis chronic continuous recurrent currents, remission stage. Partial reduction of kidney function. Dysmetabolic occultopedia nephropathy. Enuresis mixed Genesis. Complaints at admission: regular - enuresis, ultrasound changes kidney, retarded physical and mental development, occasionally pain in the right hypochondrium, headaches with fatigue and a change in the weather. Calculated IPGS the child has determined its value -13,2124. The teenager, identified "the lack of reserve capacity" due to an excess of FSH and LH. Upon further examination and consultation of the gynecologist and the endocrinologist diagnosed hypergonadotropic hypogonadism, agenesia of the ovaries, rudimentary uterus. Teen taken under supervision and treatment professionals. The calculation of IPGS the child:
Thus, clinical examples show that the proposed method provides an assessment of reproductive health in hormonal profile. Due to the fact that you are using the integrated indicator of hormonal status, including assessment of various endocrine glands, increases the reliability of the diagnosis of abnormalities of the reproductive health of adolescents suffering from chronic renal pathology.
The way to diagnose abnormalities in adolescent reproductive health, male and female with chronic renal disorders, comprising determining the level of thyroid-stimulating hormone (TSH, mked/ml), free thyroxine (CL4,pmol/l), prolactin (PRL, mIU/ml), follicle-stimulating hormone (FSH, mIU/ml), luteinizing hormone (LH, mIU/ml), testosterone (T, nmol/l), dehydroepiandrosterone-sulfate (DHEA-s, ug/ml), cortisol (nmol/l), estradiol (E2,PG/ml), 17-Oh progesterone (17-OPG, nmol/l) in serum, characterized in that they calculate the integral indicator of hormonal status by the formula
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