Ointment for skin pecilomycosis

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmacy, namely agents based on herbal raw material possessing pecilomycotic action. A sage kerosene ointment contains kerosene extract of air-dried sage leaves, Vaselin taken in certain proportions.

EFFECT: ointment possess effective antimycotic action.

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The invention relates to the pharmacy, and in particular to means on the basis of medicinal plants with antifungal activity.

Paecilomyces is a fungal disease caused by infection with various types of conditionally pathogenic fungi of the genus Paecilomyces, described in Mahonoy (Akhunov A.M., Shustov VI Clinical and immunological characteristics of paecilomyces // Clinical medicine. - 1991. No. 3. S-115).

The main reservoir paecilomyces infection in nature is the soil and in the human body - blood. The main pathways paecilomyces infection - respiratory, oral and transplacental. Mushrooms of the genus Paecilomyces saprophyticus in the soil as mycelium forms, and when injected into a human or animal are transformed into tissue parasitic form of Sterol. Mature spherule these mushrooms - rounded calf with dual-circuit shell, within which are enclosed endospores. The size of the Mature Sterol range from 10 to 29 μm, endospore from 0.3 to 1.5 μm. The disease is accompanied by severe lesions in vital organs, which can lead to death.

In officinales medicine, there are many plants with antifungal activity, one of them is the sage leaves (Folia Salviae), which contain from 0.5% to 2.5% essential oil in the composition is otorongo is cineole (up to 15%), l-α-thujone, d-β-thujone, d-α-pinene, Calvin, d-borneol (8-14%), d-camphor, adren. In addition, the leaves contain tannins, flavonoids, ursolic and oleanolic acid, vitamin e, nicotinic acid, bitter, volatile, uvaol, paracetanol. In the work Dragan T. (Chemical Composition and Antimicrobial Characteristic of the Essential Oils Obtained from the Flower, Leaf and Stem of Salvia officinalis L. // Journal of Essential Oil Research. Vol.14, N6, 2002) studied the antibacterial activity of the oils on the following microorganisms: Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Sarcina lutea, Candida albicans, Saccharomyces cerevisiae, Aspergillus niger. It is revealed that the concentration of oil, which stopped the growth of B. Subtilis, was 2%, St. Aureus - 0,2%, E. coli and P. Aeruginosa - 4%. Other strains of microorganisms oil also had antibacterial action.

Object of the invention is an ointment for the treatment of skin paecilomyces.

The problem is solved ointment for the treatment of skin paecilomyces, characterized in that it contains from 9.0 to 10.0 g of kerosene extract obtained by maceration within two weeks of the dried leaves of Salvia officinalis and aviation fuel in the ratio of 1-4:5, and vaseline to 100.0.

The following example shows how to get the ointment.

Method of maceration in the ratio of raw material and extractant 1-4:5 receive kerosene extract of the dried leaves of Salvia officinalis (humidity h is more than 14%). In a glass vessel of dark glass with a tight lid placed 5,0 dried ground leaves of Salvia officinalis (particle size 10 mm), pour aviation kerosene in the amount of 25.0 and infused for 2 weeks in a dark place with occasional stirring. Then, the resulting extract is filtered.

Kerosene extract of the dried leaves of Salvia officinalis is a clear liquid dark green colour with a pungent characteristic odor, which provides an additional 5 unidentified compounds, compared with an alcoholic extract of Salvia officinalis.

Next, in a water bath melt some vaseline (40,0) and dissolve in it a kerosene extract, Salvia officinalis (10,0). Then add the remainder of vaseline (to 100.0) and stirred until a homogeneous consistency.

Test the obtained ointment toxicity.

The experiments were carried out on randombred white albino male rats (weighing 210-220 g). Groups of rats was 30 individuals (10 of each study period). Ointment sage-kerosene and clean sage-kerosene extract was applied to the shaved skin of animals in the amount of 0.2 g daily for 30 days. The area of application was 1 cm². During the first 14 days of the experiment at ptopic the s rats was not registered deviations from control in all biochemical parameters. However, after 30-day exposure to kerosene sage extract was observed reversible signs of local irritation in the form of hyperemia of the skin and the appearance of thin crust. When applying the test ointments such phenomena wore a single character. In a study of rats after application of kerosene extract of sage observed changes in hematological parameters (reduction of the color indicator and the acceleration of blood clotting). In the group of rats to which was applied the test ointment, fluctuations in hematological and biochemical parameters did not differ from the control groups of animals.

Test paecilomyces activity was performed, comparing the effectiveness of sage-kerosene ointment with nizoralbuy ointment used for skin paecilomyces. Comparison of ointments was carried out on 60 outbred white rats-males weighing 210-230 g

In total, the 39-x rats in the experiment developed skin paecilomyces. Before treatment was performed hematological and biochemical analysis of the blood of animals (table 1, 3). As can be seen from the given data, in rats, infected skin paecilomyces marked in the blood decrease in the number of erythrocytes, hemoglobin, total protein and white blood cells, increase in the number of eosinophils, glucose, the activity of Alat, ASAT, alkaline phosphatase.

17 rats treated - sage-kerosene ointment is, and 14 rats - product comparison - nizoralbuy ointment.

Within 30 days daily from 17 rats smeared sage-oil ointment in a thin layer in the morning and in the evening the affected skin. All animals disappeared purple areas and has cured, the same effect obtained in the treatment of 14 rats nizoralbuy ointment. Animals normalized blood in the group that was treated sage-oil ointment. In the group of rats treated with nizoralbuy ointment, came normalization of blood counts (tables 2, 4). Thus, unlike nizoralbuy ointment sage-kerosene ointment provided impact not only on the local site of infection, but the lesions that developed in the lungs.

In addition, for a variety of purposes were infected from patients paecilomyces animals 40 white mice weighing 18-20 g in a variety of ways of introducing the culture of paecilomyces mushrooms: intraperitoneally, subcutaneously and just inside. 37-mi mice were amazed mushrooms tails. They were divided into three groups: 7 was a control that was not treated (the first group), 12 mice, the tails of which were processed by nizoralbuy ointment (the second group), 18 mice tails are smeared sage-oil ointment. Within 5 days all 7 mice of the first group died from paecilomyces. In the second group died 7 mice at 6 and day 7 after treatment, in the third group all 18 mice recovered. Therefore, sage-kerosene ointment has not only the effect of treatment of skin paecilomyces, but also a strong antifungal activity.

Ointment for the treatment of skin paecilomyces, characterized in that it contains from 9.0 to 10.0 g of kerosene extract obtained is about at the insistence within two weeks of the dried leaves of Salvia officinalis and aviation fuel in the ratio of 1-4:5 and vaseline to 100.0 g