Antibacterial gel

FIELD: cosmetology.

SUBSTANCE: the present innovation deals with cosmetic preparation for taking care of skin, in particular. The suggested antibacterial gel consists of either hydroxypropylcellulose or hydroxyethylcellulose, propylene glycol or diethylene glycol compound, polyguanidine or its synergistic mixture with quaternary ammonium compound, chitosan, oxyethylated ether of sorbitane, polyvinyl pyrrolidone at molecular weight ranged 10000 - 40000 or copolymer of polyvinylpyrrolidone, trilon-B - sodium salt of ethylene diamintetraacetic acid, perfumery composition, vitamin constituent and water. The present innovation provides increased regenerating formula of skin and its enhanced barrier function, improves homogeneity of the composition mentioned and its stability.

EFFECT: higher efficiency of application.

5 cl, 4 ex, 3 tbl

The invention relates to the field of cosmetology, in particular to the means for skin care, and can be used for skin care of the face, hands, feet and other body parts.

Known gel for antiseptic treatment of skin, containing in its composition modified cellulose derivative, antiseptic - Miramistin and water (U.S. Pat. Of the Russian Federation No. 2185157, class a 61 K 31/14, 2000).

However, used Miramistin not have a prolonged effect and is effective for a narrow field of use.

The closest solution to the present invention is a hygienic gel of the following composition, wt.%: the carbopol - 0,1-10,0; triethanolamine - 0,1-10,0; liposomal drug “Witch-2” (protivougrevoe) is 1-30; glycerin distilled PC-94 - 1-15; ethyl alcohol of the highest clean - 0,1-5,0; water-spiropiranovye burdock extract - 0,1-5,0; water-spiropiranovye the horsetail extract - 0,1-5,0; triclosan - 0,1-1,0; camphor synthetic - 0,01-1,0; dye C1 42090 - 0,1-1,0; fragrance composition is 0.1 to 1.5; drinking water - the rest (U.S. Pat. Of the Russian Federation No. 2150264, CLA 61 K 7/00, 1999).

The disadvantages of this gel is that applied triclosan is deklarirovannoe derived bis-phenol, similar in structure to extremely dangerous substance dioxin. It is known that triclosan may contain a mixture of dioxin - extremely Tox is knogo and hazardous substances. In addition, it is known that chlorinated phenols by simply heating easily turn into dioxins. At the same time, the antibacterial activity of triclosan is extremely low.

The technical problem solved by this invention is to reduce the toxicity of the gel through the use of low-toxic disinfectants with improved dermatological action and prolonged effect, enhancing the regenerative functions of the skin and strengthen its barrier function. In addition, improving the homogeneity of the composition and its stability.

To solve the technical problem in the antibacterial gel, including alkylhydroxylamines connection, disinfectant, perfume composition, and water, as alkylhydroxylamines connections use propylene glycol or diethylene glycol, as a disinfectant is used as a compound of polyguanidine or synergistic mixture of Quaternary ammonium compounds and optionally enter a hydroxyethyl cellulose or hydroxypropylcellulose, chitosan, ethoxylated ether sorbitan, polyvinylpyrrolidone with a molecular weight of from 10000 to 40000 or copolymer of polyvinylpyrrolidone, Trilon B - sodium salt of ethylenediaminetetraacetic acid, a vitamin component, and water in the following ratio of components, wt.%:

- hydroxyprop cellulose or

hydroxyethylcellulose 0,3-3,0

- propylene glycol or diethylene glycol and 0.2 to 15.0

connection of polyguanidine or synergistic

a mixture of Quaternary ammonium compounds

in a 1:1 ratio 0,5-3,0

- chitosan 0,1-2,0

- polyoxyethylene ether sorbitan 0,01-1,0

- polyvinylpyrrolidone with a molecular weight of from 10,000

up to 40,000 or copolymer of polyvinylpyrrolidone 0,01-0,5

- Trilon B - sodium salt

ethylenediaminetetraacetic acid of 0.1-2.0

- perfume 0,2-0,5

vitamin component of 0.1-2.0

- water the rest

The antibacterial gel as polyguanidine use phosphate poly-(4,9-dioxadodecane-1,12-guanidine), or citrate/formate, guanidine, or gluconate/benzoate, guanidine, or chloride, guanidine.

The antibacterial gel as a Quaternary ammonium compound used dimethylbenzylamine-sulfate, as well as ethoxylated ether sorbitan use tween-20 or tween-40 or tween-60, or tween-80.

The antibacterial gel as a vitamin component use vitamin a, or vitamin E, or vitamin In₅or their mixture in equal parts.

Used as the gel is a highly active compound of polyguanidine or synergistic mixture of Quaternary ammonium is soedinenie - dimethylbenzylamine-sulfate has a broad spectrum of biocidal activity: antibacterial, fungicidal, virucidal. It allatoxin, does not penetrate intact skin (IY hazard class), is stored on it for a long time, protecting against attacks of microorganisms.

Dimethylbenzylamine-sulfate is synthesized in two stages, based on benzylamine, dodecylamide and dimethylsulfate.

First heated at a temperature of 60-80°With an alcohol solution of 97 g (1 mol) of benzylamine with 205 g (1 mol) of dodecylamine and 40 g (1 mol) sodium hydroxide before the termination of the precipitated NaCl. The solution benzyldimethylamine filtered off from the precipitate of NaCl and quaternized added 126 g (1 mol) of dimethylsulfate and 20 g (0.5 mol) of caustic soda.

The resulting dimethylbenzylamine-sulfate dried at a temperature not above 60°C to constant weight. Its antimicrobial activity of the drug exceeds katamin AB and benzalconi-chloride.

Used in the proposed composition as a gel-forming component hydroxypropylcellulose or hydroxyethylcellulose refers to the non-ionic cellulose ethers and is a powdery white material with a main component content not less than 97 wt.%, the degree of polymerization of not less than 500 (THE 6-05-1857-87).

Noted the haunted esters of lower alcohols - propylene glycol (TU 6-09-2434-81) and diethylene glycol (GOST 10136-77) have preservative, antiseptic and bactericidal properties. The absorption of propylene glycol allows you to keep the gel to the desired consistency. Introduction in the gel propylene glycol increases the permeability through the skin.

Used in the proposed composition of the chitosan - poly,4-2-amino-2 deoxy-D-glucans (THE No. 9289-002-11418234-99) is a cationic polysaccharide, a structural analogue of cellulose, obtained by deacetylation of chitin, a natural biopolymer, which is part of the shells of crustaceans and insects, forms the cellular structure of most fungi and some algae. Chitosan stimulates the body's cells involved in immunological protection against pathogenic microorganisms, accelerates the healing of wounds. Chitosan is introduced into the gel as an immunomodulator, a viscosity regulator, an antiseptic and a modifier. In addition, it enhances the action of the main antibacterial component polyguanidine or synergistic mixture of Quaternary ammonium compounds.

Ethoxylated esters sorbitan (sorbital, twins) are nonionic surfactants. They are distinguished by a member of the molecule acid (stearic, oleic, lauric, palmitic) and the degree of oksietilirovannye (5 or 20). They races is Vratsa in water, ethanol, benzene, but insoluble in petroleum ether, mineral oil.

Tween-80 (sorbitan O-20)-serviceorienteret is a light yellow oily liquid with a basic substance content of 98% (FS 42-2540-88).

Tween-60 (sorbitan C-20)-serviceprocessinstaller is a light yellow oily liquid with a basic substance content of 96% (THE 6-14-1050-81).

Twin room-40 (TL-N) and tween-20 (TL-W) - products company Cao Chemical, Japan.

Ethoxylated esters sorbitan introduced to improve the penetrating power of the gel and improve the stability of the composition.

Polyvinylpyrrolidone (molecular weight of from 10000 to 40000) is a white powder, easily soluble in water and most organic solvents (FS 42-2238-98).

Polyvinylpyrrolidone (PVP) tells the gel prolonged action and is a known detoxifier (gemodez). In combination with polyguanidine forms complexes with reduced toxicity. In addition, PVP in gel alters the structure of water in which is dissolved GHG that contributes to the stability and homogeneity of the gel.

Used copolymers of polyvinylpyrrolidone with acrylates, substituted alkylamines followed, are the products of the company ISP VAN DYK (USA):

poly(vinyl pyrrolidone/dimethylaminoethylmethacrylate) - COPOLIMER845; and COPOLIMER - 937; poly(vinyl pyrrolidone and metallicametallicametall) - POLIQUTENIUM-28 and poly (vinyl pyrrolidone/dimethylaminoethyl methacrylate) - POLIQUTENIUM - N.

Trilon B (GOST 10652) is a sodium salt of ethylenediaminetetraacetic acid, complexone III is an organic chelate forming compound, crystalline, soluble in water, ethanol and most organic solvents.

Used as a titrant in complexometry, for division and separation of metal ions to dissolve and prevent the formation of various sediments, caused, for example, water hardness, for excretion from the body of toxic metals, stabilization of food, water softener, as components of detergents.

Fragrance composition is an oily liquid with a characteristic smell and is introduced into the gel to give it a pleasant odor.

Use perfume composition with the following scent: Apple, peach, apricot, banana, vanilla, cherry, grapefruit, Jasmine, honeysuckle, cocoa, cinnamon, kiwi, lavender, Lily of the valley, lemon, lime blossom, mango, chamomile, calendula, green tea, fruity floral and fruit-herbal (mint, burdock, St. John's wort, rosemary, tarragon, eucalyptus, sage, thyme, hops, violet, series).

Manufacturers can be: FF (Italy), “Bell flowers and fragrances (Germany) or the company's Phytotechnology” (St. Petersburg).

Vitamin a affects the metabolic processes of the skin, has a healing effect.

Provitamin₅(panthenol) stimulates the renewal of skin.

Vitamin E has a regenerative effect, protects the immune system, is an effective antioxidant that stabilizes the composition.

The quantitative content of the components of the gel is optimal and selected on the basis of numerous experiments. In table 1 presents data confirming the optimality of the declared limits.

The process of preparation of antibacterial gel has the following stages. The first is the preparation of raw materials and equipment - includes checking the purity of the reactor mixing and weighing ingredients. The second stage is the dispersion of non-ionic cellulose ethers (hydroxyethyl cellulose or hydroxypropylcellulose) in diethylene glycol or propylene glycol for 30 minutes at a temperature of 35-40°with periodic mixing. The mixing mode and the design features of the reactor is supposed to prevent foaming.

The third stage - sequential introduction with stirring the resulting dispersion weighted amount of water, ethoxylated ether sorbitan, trylon B, chitosan, polyvinylpyrrolidone or with whom alimera. Next, the mixture is heated to a temperature of 35-40°C for 2-4 hours until a homogeneous viscous solution.

The fourth stage is the preparation of aqueous disinfectants in the mixer at a temperature of 30-40°C for 1.5-2 hours.

The fifth stage is the mixing of the components in the reactor-mixer, which is a cylindrical vessel with cooled jacketed stainless steel with a ratio of diameter and height of 1-1,5:1-2, provided with a removable cover, intake fittings and anchor stirrer or turbine type. In the reactor pour the prepared homogeneous viscous solution obtained in the third stage of the process, an aqueous solution of disinfectant, perfume composition and a vitamin component. In the resulting mass of water is added and continuously stirred at a temperature of 25-30°C for 1 hour.

Examples of specific performance of a gel.

Example No. 1. Gel antibacterial hand contains the following components, wt%:

- hydroxypropylcellulose 1,5

- propylene glycol 7,0

- phosphate poly(4.9 dioxadodecane 1,12-guanidine) 1,5

- chitosan 1,0

twin 20 0,5

- PVP 0,3

- Trilon B 1,0

- perfume 0,3

- provitamin₅1,0

- water the rest

Used for protection of the skin against the penetration of germs and bacteria. After application of the gel is and the skin is formed thin film, which doesn't dry the skin and disrupts the pH balance.

Example No. 2.

The antibacterial gel for face contains the following components in wt.%:

- hydroxypropylcellulose 0,3

- diethylene glycol 15,0

- citrate/formate pgmg 0,5

- chitosan 2,0

twin 60 0,01

- PVP 0,01

- Trilon B 0,1

- perfume 0,1

the mixture of vitamins E and a in the ratio of 1:1 to 2.0

- water the rest

Used for problem skin, irritating and allergic action.

Example No. 3. The antibacterial gel for feet contains the following components in wt.%:

- hydroxyethylcellulose 3,0

- propylene glycol 0,2

- synergistic mixture of chloride pgmg and

dimethylbenzylamine sulfate 3,0

- chitosan 0,1

twin 60 1,0

- PVP copolymer with molecular weight of 10000 0,5

- Trilon B 2,0

- perfume 0,5

- provitamin₅, vitamin E, vitamin a

in the ratio of 1:1:1 0,1

- water the rest

Eliminates the smell of sweat, protects from infection by fungus and other skin diseases, kills pathogens.

Example No. 4

- hydroxypropylcellulose 2,5

- propylene glycol 12,0

- gluconate/benzoate pgmg 2,5

- chitosan 1,5

twin 80 0,05

- PVP 0,3

- Trilon B 0,5

- perfume 0,4

- vitamin And 1.5

- water the rest

Eliminates the smell of sweat, the sight is preserved from contamination by fungus and other skin diseases, kills pathogens.

Testing of antimicrobial activity developed antibacterial gel was determined by zone of inhibition of microorganisms by diffusion of the analyte in a dense nutrient medium (Fedorov Y.A., Root NR. “Microbiological research methods in dental hygiene products” in the book. “Basics of oral hygiene”. L., “Medicine”, 1973, p.3-215).

The Petri dish was filled with 20 ml of a dense nutrient medium. Prepared 24 hours a microbial suspension of test microorganism with a concentration of 5·10⁵CFU/ml and applied to 1 ml of the suspension on the surface of the medium. Was dried for 30 min, and then in a nutrient medium made holes with a diameter of 6 mm, which was applied to the samples developed disinfectant gel. The number of holes should not exceed 6 per Petri dish. For 30 min in Petri dishes kept at room temperature (20°C), then they were placed in the incubator for 24-48 hours at a temperature of 37°and upon expiration of that period took into account the zone of inhibition of microbial growth, including the diameter of the hole. The size of zones of inhibition of growth of test microorganisms was judged on the antimicrobial activity of the test substance, although it should be considered that the diffusion rate of the polymeric disinfectants lower rate of diffusion belongs to low-polymeric who disinfectants.

To obtain reliable test results for each analyte should be done in 2-3 series of replications.

Determining the minimum time required for complete suppression of the growth and development of test organisms, were developed for antibacterial gel as follows.

In the test substance was added to a suspension of the test organisms in the amount of 10⁵CFU/ml in a test tube pour 1 ml of the test substance. The tubes were shaken and intervals 2-5-10 and 30 minutes did the crops in the dense medium. Petri dishes were placed in 24 to 48 hours in a thermostat at 37°C. After the expiration of that period were counting the number of grown colonies and this indicator was judged on the bactericidal activity of the test substance.

The results of these studies are presented in table 2.

From the presented data in table 2 it follows that a more sensitive test means were gram-positive organisms (S. aureus). All test tools formed a zone of stunted growth from 19 to 24 mm, which corresponds to a relatively high activity. Draws attention to the high activity of the preparation according to example 3 to gram-negative microorganisms of the species E. coli and Ps.aeruginosa.

When determining the speed of the manifestations of antimicrob the activity to experience funds were obtained, presented in table 3.

From the presented data in table 3 it follows that the antibacterial effect of the developed formulations is developing very quickly. After 2 minutes of contact in any case not found living microflora after contamination by Escherichia coli.

Testing of disinfectant gel was carried out on 100 volunteers without burdened allergological anamnesis within 30 days. According to a patchwork of samples and sample application shows that the gel is harmless, irritating and allergenic action.

1. The antibacterial gel, including alkylhydroxylamines connection, disinfectant, perfume composition, and water, characterized in that as alkylhydroxylamines connections use propylene glycol or diethylene glycol, as a disinfectant is used as a compound of polyguanidine or synergistic mixture of Quaternary ammonium compounds and further comprises hydroxyethyl cellulose or hydroxypropylcellulose, chitosan, ethoxylated ether sorbitan, polyvinylpyrrolidone with a molecular mass of capabilities from 10,000 to 40,000 or copolymer of polyvinylpyrrolidone, Trilon B is the sodium salt of ethylendiaminetetraacetic the th acid, vitamin component, and water in the following ratios, wt.%:

hydroxypropylcellulose or

hydroxyethylcellulose 0,3-3,0

propylene glycol or diethylene glycol and 0.2 to 15.0

the connection of polyguanidine or

synergistic mixture of Quaternary

ammonium compound in the ratio

1:1 0,5-3,0

chitosan 0,1-2,0

ethoxylated ether sorbitan 0,01-1,0

polyvinylpyrrolidone with a molecular

mass capabilities from 10,000 to 40,000 or copolymer

polyvinylpyrrolidone 0,01-0,5

Trilon B-sodium salt

ethylenediaminetetraacetic acid of 0.1-2.0

fragrance composition of 0.2-0.5

vitamin component of 0.1-2.0

water the rest

2. The antibacterial gel according to claim 1, characterized in that compounds of polyguanidine use phosphate poly-(4,9-dioxadodecane-1,12-guanidine), or citrate/formate, guanidine, or gluconate/benzoate, guanidine, or chloride, guanidine.

3. The antibacterial gel according to claim 1, characterized in that the Quaternary ammonium compound used dimethylbenzylamine-sulfate.

4. The antibacterial gel according to claim 1, characterized in that as a polyoxyethylene ether sorbitan use tween-20, is whether the twin 40, or tween 60 or tween 80.

5. The antibacterial gel according to claim 1, characterized in that as a vitamin component use vitamin a, or vitamin E, or vitamin In₅or their mixture in equal parts.