Method for integrated assessment of genetic predisposition to development of psychoactive substance abuse. RU patent 2505820.
 Method for typing inflammatory rheumatic disease using joint fluid / 2504788Invention describes a method for typing in vitro an inflammatory rheumatic disease, namely for differentiating rheumatoid arthritis and arthritis related to skin disease, wherein the presence or absence of joint fluid Hdj2 protein at the early stage of the disease, i.e. for a period of time up to two years from the onset of first symptoms of inflammatory rheumatoid arthritis, is determined. What is also described is using Hdj2 protein monoclonal antibodies for the purpose of differential diagnosis in vitro of the inflammatory rheumatic disease. |
 Diagnostic technique for urolithiasis / 2504786Original urine sample is divided into two equal samples; particle size histogram is prepared and used to find a percentage of an oligomer form of Tamm-Horsfall protein T&HE(7); the sample histograms are compared. A Tamm-Horsfall protein identification protein is presented by monoclonal antibodies undergoing an immune-affine reaction with Tamm-Horsfall protein. The second sample is used to recover Tamm-Horsfall protein T&HE(28); and both urine samples are used to measure the oligomer form of Tamm-Horsfall protein T&HE(28) in the free condition and in aggregates with oxalate microcrystals (%). The relation of the derived oligomer forms is calculated by formulas: C1=T&HE(28)A/T&HE(7) and C2=T&HE(28)F/T&HE(7). If observing C1>1.5 and any C2, urolithiasis is diagnosed; the relations C1<0.1 and C2<1.81 enable diagnosing the absence of the disease, while the relations C1<1.5 and C2>1.81 make the patient be referred to a risk group of developing urolithiasis. The technique enables distinguishing between those suffering urolithiasis and healthy ones among the persons being tested, and also separating a risk group of developing urolithiasis. |
| Method for individual prescription of systemic glucocorticosteroid pulse therapy in patients with endocrinal ophthalmopathy / 2503011 Method for individual prescription of the systemic glucocorticosteroid pulse therapy in the patients with endocrinal ophthalmopathy is implemented by assessing the disease activity by CAS scale, and examining patient's blood serum for heat shock protein 90. The disease activity of 3 points and more in a combination with the heat shock protein 90 less than 38 ng/ml, the systemic glucocorticosteroid pulse therapy is to be prescribed. |
| Diagnostic technique for patients with lumbar osteochondrosis / 2503010 Blood serum anti-inflammatory cytokines concentrations of tumour necrosis factor-α (TNF-α), interleukin-1 (IL-1) and interleukin-2 (IL-2) are determined in the patients with vertebral disorders. If observing FNO-α more than 3.6±0.6 pg/ml, IL-1 more than 53.2±8.2 pcg/ml, IL-2 less than 319.2±11.03 pcg/mg, lumbar osteochondrosis is diagnosed. |
 New wheat allergens / 2502742Allergens are used in a diagnostics method of IgE-mediated allergy in vitro for wheat, which involves contact of a liquid sample of a mammal organism that is supposed to have IgE-mediated wheat allergy at least with one polypeptide with SEQ ID NO: 2 or 8, or with its fragment or variant having common epitopes for antibodies with the specified polypeptide and having the sequence identical to SEQ ID NO: 2 or 8, at least by 95%; and reveal of presence in the sample of IgE-antibodies that are specifically linked to the above polypeptide or polypeptides. Presence in the sample of such antibodies is a sign of IgE-mediated wheat allergy. Diagnostics set includes the above polypeptides and means for reveal of IgE connection to the above polypeptide, such as a hard substrate, for example nitrocellulose membrane or micromatrix containing a polypeptide linked to it. |
 Method for obtaining therapeutic agent for curing of tick-borne encephalitis / 2501862Method involves production of aptamers that are capable of forming a complex with tertiary structure of surface virus protein. Fragment of surface protein E of tick-borne encephalitis virus, aminoacid from 50 to 250 from N-end of the protein, the gene of which is amplificated and cloned in a plasmid vector, is used. Cloned recombinant protein is expressed. The obtained protein is cleaned and immobilised on a plane table. From initial pool of degenerated oligonucleotides composed of 5'-CTCCTCTGACTGTAACCACG-(N40)-GGCTTCTGGCTACCTATGC-3', where N - any of nucleotides (G, A, T, C) taken in equivalent amount at synthesis of 40-link oligonucleotides containing fluorescent colourant FAM on 5'-end by means of 15 cycles of exponential benefication, SELEX, there performed is extraction of aptamers specifically bonded to virus protein. At each sorption stage there performed is denaturation of 25 pM single-stranded oligonucleotide. Denaturated pool of aptamers is applied onto a basin with immobilised protein, incubated and washed from non-bonded oligonucleotides. Aptamers bonded to protein are removed from surface with buffer with low ionic force and high pH. Pool of aptamers is amplificated after each sorption stage. Obtained amplicons of two-stranded library of aptamers are cleaned and a reaction of asymmetric polymerase chain reaction (PCR) is performed. The product is cleaned by means of sorption so that target product is obtained in the form of aptamers. |
| Method for prediction of epilepsy severity / 2499262 Patients' blood serum is examined for the pre-therapeutic mean molecule spectrum. If a mean molecule fraction described as an optical absorption E at wave length 230 nm is below 0.118 standard units, and a nuclear-peptide index described as a relation of the optical absorption E at wave length 230 nm to the optical absorption E at wave length 254 nm is below 0.8, the severe clinical course of epilepsy is predicted. |
| Method of peptide sequencing by mass-spectrometry and defining of their amino acid sequences / 2498443 Method of peptide sequencing by mass-spectrometry and defining of their amino acid sequences is based on fragmentation in mass-spectrometer ion source between the nozzle and skimmer for molecular ions of peptides under the action of controlled electric field and subsequent mass-spectrographic analysis of fragments. Peptide comes to the ion source which electrogasdynamic system allows control of fragmentation degree for molecular ion by means of electric field changing. Further ions are divided in mass analyser and delivered to detector where mass spectrum of the peptide and its fragments with different fragmentation depth in the same spectrum is registered. Mass spectra of the peptide fragments obtained at different values of the electric field force are processes by the recording system, analysed and amino acid sequence is defined for the source peptide in result. Controlled degree of fragmentation in the ion source under action of the varied electrical field within the range of 102-104 V/m and residual gas pressure within the range of 100-2000Pa allows determination of amino acid sequence of peptides containing up to 10-15 amino acid residues which corresponds to an average length of peptides, products of protein enzymatic hydrolysis. |
| Method for prediction of clinical course of disease in patients with soft tissue sarcoma / 2498315 What is presented is a method for prediction of the clinical course of the disease in the patients with soft tissue sarcoma that involves the biochemical study. The check-up examinations follow every three months in the patients suffering soft tissue sarcoma, and blood serum is tested for the content of C-reactive protein (C-RP). If the C-RP values is within the range of 1.94 to 3.25 mg/l with an average value of 2.56 mg/l, the process stabilisation is predicted; then the C-RP value falling within the range of 5.18 to 45.45 mg/l with an average value of 14.24 mg/l, the onset of the distant metastases is 3-6 months is predicted, while the C-RB value being 6.17 to 95.00 mg/l with an average value of 29.66 mg/l, the presence of the distant metastases and the process generalisation are predicted. |
| Method for prediction of risk of disturbed fertility in males suffering obesity / 2498314 What is presented is a method for prediction of a risk of disturbed fertility in males suffering obesity, involving venous blood sampling followed by the allele-specific polymerase chain reaction using a fluorescent labelled oligonucleotide probe and melting line analysis. Having found the allele A of CYP19A aromatase gene polymorphisms rs2414096 and rs749292 enables predicting the risk of disturbed fertility in males suffering obesity. |
 Method for predicting the character of bacterial keratitis flow / 2245553In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow. |
| Method for detecting the sequence of applied lesions / 2245555 For the purpose to detect the sequence of applied lesions at availability of several wounds, scratches and ecchymoses on a cadaver one should study the activity of alkaline peptides isolated out of affected tissue by the impact of blood neutrophils of healthy donors upon phagocytosis. Moreover, the highest stimulating effect belongs to the peptides isolated out of the lesion applied earlier. The method enables to detect the sequence of applied lesions more accurately and differentiate the repeated lesion applied 5 min later, or more. |
| Method for biochemical detecting the degree of chronic hepatitis activity / 2246112 In blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity. |
 Early diagnosis method for diagnosing external genital endometriosis in women / 2247391Method involves determining cathepsin D activity in endometrium bioptate. The value being equal to or less than 0.1 units of enzymatic activity per hour, external genital endometriosis is diagnosed. |
| Method for estimating enteric detoxication in the cases of generalized peritonitis / 2247392 Method involves studying lactoferrin content in blood serum and peritoneal exudates in postoperative period every day during the first three days. Lactoferrin concentration in blood serum being concurrently reduced by 0.02 mcmole/l or less and increasing lactoferrin concentration in peritoneal exudates by 0.04 mcmole/l or more, enteric detoxication is considered to be effective. |
| Method for diagnosing septic process and predicting septic complications development in children / 2248572 Method involves determining plasminogen/plasmin, α2-macro-globulin, α1-antitripsin content at the first, third, fifth and tenth day. The plasminogen/plasmin level being equal to 66-74 mcmole/l or 100-120 mcmole/l, α2-macro-globulin level of 2.7-3.0 mcmole/l, α1-antitripsin content of 2.38-3.2 mcmole/l, systemic inflammatory response to purulent infection, light severity degree endotoxicosis is diagnosed and favorable disease outcome is predicted. The plasminogen/plasmin level being equal to 50-65 mcmole/l or 125-160 mcmole/l, α2-macro-globulin level of 2.3-2.6 mcmole/l, α1-antitripsin content of 3.3-4.0 mcmole/l, sepsis with organ and system dysfunction, moderate severity degree endotoxicosis is diagnosed and septic complication availability and lingering disease development course is predicted. The plasminogen/plasmin level being equal to 39-40 mcmole/l, α2-macro-globulin level of 1.58-2.08 mcmole/l, α1-antitripsin content of 5.0-6.2 mcmole/l, severe sepsis, septic shock, severe degree endotoxicosis is diagnosed and unfavorable disease outcome is predicted. |
 Method for detecting oxidized tryptophan metabolites at endogenic intoxication / 2249219At testing one should precipitate high-molecular compounds with acetonitrile and register supernatant's spectral characteristics. Supernatant should be applied onto a paper filter, dried and put into solution containing aromatic aldehyde, acetone and concentrated hydrochloric acid taken at weight ratio of 70:5:1 to be kept for 2-3 min. Then it should be once again dried up to detect qualitative and semiquantitative content of oxidized tryptophan metabolites by intensity and chromatic shades. Moreover, by chromatic shades of yellow dyeing it is possible to detect the content of hydroxylated metabolites and by chromatic shades of violet dyeing - that of unhydroxylated ones. |
| Method for predicting unfavorable result of metastatic peritonitis / 2251700 In patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result. |
| Method for diagnosing endotoxicosis condition in cows suffering from acute pyocatarrhal endometritis / 2252418 Method involves determining low and middle molecular mass substances content in blood plasma and erythrocytes and general blood plasma albumin concentration. Integral index is calculated on basis of obtained values using formula II=100*S238-298(plasma)/S238-298(erythrocytes)*GAC, where S238-298(plasma) and S238-298(erythrocytes) are the low and middle molecular mass substances content in blood plasma and erythrocytes, respectively, determined from area of figures restricted by spectral curves in wavelength range of 238-298 nm and abscissa axis (conditional units2); GAC is the general blood plasma albumin concentration (g/l). The value being from 2.1 to 3.0, the first endotoxicosis degree is diagnosed. The value being from 3.1 to 4.5, the second endotoxicosis degree is diagnosed. The value being from 4.5 to 6.0, the third endotoxicosis degree is diagnosed. The value being greater than 6.0, the fourth endotoxicosis degree is diagnosed. The normal value is equal to 0.5-2.0. |
| Method for evaluating inflammatory process activity in infantine osteomyelitis cases / 2252419 Method involves separating blood serum proteins into fractions, determining albumins and alpha-2-globulins content and controlling their content changes during the disease development process. Gamma-globulin content is determined in per cent ratio with respect to total protein quantity. Then, changes in the fractions content are controlled from the first to the third week. Albumin content being in norm and alpha-2-globulins content becoming greater to the end of the first week by 30-50% when compared to normal value and dropping to norm at the second week end and gamma-globulin content increasing from norm by 10-30% to the second or the third week, high inflammatory process activity is to be diagnosed. Albumin content dropping by 10-30% from normal value at the second week, alpha-2-globulins content growing by 10-20% of norm and gamma-globulin content dropping by 30-50% at the second or the third week when compared to norm, low inflammatory process activity is to be diagnosed. |
FIELD: medicine.
SUBSTANCE: polymerase chain reaction is used in biological objects to determine allele G in locus rs 1042173 of gene SLC6A4 of the serotonin neurotransmitter system, and an allele specified in a group of genes of the dopamine neurotransmitter system: G in locus rs4680 of gene COMT, G in locus rs 17851478 and/or C in locus rs 1611115 of gene DBH, C in locus rs6275 of gene DRD2. If observing at least one risk allele in the both systems, a genetic predisposition to the development of psychoactive substance abuse is stated.
EFFECT: more accurate assessment of the risk of psychoactive substance abuse ensured by selecting the optimal system of neurotransmitter markers.
6 ex
The invention relates to medicine, namely to the narcology, and can be used to identify among population groups with high biodiversity risk of addiction to psychoactive substances (SAS)to provide for a differentiated prevention with the inclusion of socio-psychological and educative programs for the entire population and special for individuals with a predisposition to addiction diseases.
Clinical practice shows that not all members of a family having the genetic predisposition to abuse (SAS), is developing alcoholism or drug addiction.
In this connection special value gets search «markers»predictors for the identification of persons with a biological predisposition to drug surfactants.
Direct causal relationship between a specific gene and addiction in General are absent.
Several studies have shown a positive Association between a single replacement nucleotide 118A→G (rs 1799971) gene mu-opioid receptor 1 (OPRM1) and opioid dependence, as well as to dependence on other substances in different populations (Bart, G. et al. 2004; Kapur, S. et al. 2007), while other studies have not found this regard, the SNP (Yuferov, V. et al. 2010).
Examination of the relationship alleles TaqI Al gene dopamine receptor type 2 (DRD2) alcohol use remains controversial, because of contradictory results.
(Neville MJ, Johnstone EC, Walton RT: Identification and characterization of ANKK1: a novel kinase gene closely linked to DRD2 on chromosome band 11q23.1. Hum Mutat 2004, 23:540-45.
Blum K, Noble EP, Sheridan PJ, A Montgomery, Ritchie T, Jagadeeswaran P, Nogami H, Briggs AH, Cohn JB: Allelic association of human dopamine D2 receptor gene in alcoholism. J Am Med Assoc 1990, 263:2055-60.
Lucht M, Barnow S, Schroeder W, Grabe HJ, Rosskopf D, Brummer C, John U, Freyberger HJ, Herrmann FH: Alcohol consumption is associated with an interaction between DRD2 exon 8 A/A genotype and selfdirectedness in males. Neuropsychobiology 2007, 56:24-31.)
Research Prasad et al. indicate little occurrence frequency of genotype Taq A1 A1. However, the statistical analysis showed a significant (credible) communication genotype Taq A1 A1 with alcohol dependence (Fisher's p<0.05).
Thus, the combination of types of inheritance predisposition and nature of the diseases depending make methodically incorrect search only or main «gene dependence».
There is a method of complex determination of genetic predisposition to the development of psychoactive substance dependence, including the study of the complex of genes coding for the main elements of the dopaminergic system ( O.A. Genetics of substance abuse: a molecular-genetic profile of the dopamine neyromediatornoy system with alcoholism and opium addiction. // Narcology, 2011. №9. P.25-42).
However, the study only complex genes encoding the main elements of the dopaminergic system, does not give a sufficiently full picture of the genetic predisposition, resulting due to the lack of accuracy of its determination.
Known (VERDE Z. et al. ′Smoking genes′: a genetic association studies. PLoS One. 2011; 6(10)) way to determine the possibility of use of the definition in biological objects patient by the method of polymerase chain reaction polymorphic variants of genes of the two systems: dopaminergic (DRD2-ANKK1 2137G>A) and opioid (OPRM1 118A>G) for assessing the genetic predisposition to nicotine addiction.
However, the contribution of each gene was made separately, not in the complex. Found a statistically significant difference in the groups of smokers and non-smokers only DRD2 gene-ANKK1 2137G>A OPRM1 118A>G of this difference is not installed. Thus, in this source refers to the possibility of the use of the dopamine gene (DRD2-ANKK1) system for assessing the predisposition to nicotine addiction. The indicator OR (i.e. the ratio of the frequencies of alleles of risk in smokers to the same frequency in non-Smoking) in the case of DRD2-ANKK1 value of 3.6.
There is a method (Abstract; D. CHEN et al. Association between polymorphisms of DRD2 and DRD4 and opioid dependence: evidence from the current studies. Am J Med Genet In Neuropsychiatr Genet. 2011 Sep; 156B(6):661-670), which explored the relationships presence of polymorphic genes of the dopamine system and predisposition to dependence on opiates. Values OR were determined in each gene separately (no comprehensive assessment of the influence of genes), and the maximum OR amounted to 2.06 for DRD2 gene.
There is a method adopted for the prototype, which reveals the way to identify a predisposition to addiction to psychoactive substances, including the identification of biological objects of the patient by the method of polymerase chain reaction alleles risk in polymorphic genes dopamine, serotonin, and acid systems exchange, and in detecting polymorphic genes in each of neurotransmitter systems, in combination with the results of psycho-diagnostic testing, judge insufficiency exchange of this system (patent RU 2402974 C2, IPC publ. 10.11.2010 example 6, formula 1).
However, a complete picture of the genetic predisposition to dependence on substances this method is not independent, and the accuracy of its definition is insufficient.
The present invention solves the problem of increasing the accuracy of the objective, early ways to determine genetic predisposition to the development of dependence, as well as proactive clinical prediction.
The problem is solved by way of complex determination of genetic predisposition to the development of psychoactive substance dependence, including determination in biological objects patient by the method of polymerase chain reaction allele G locus rs1042173 SLC6A4 serotonin system exchange and at least one allele, selected from a group of genes of the dopamine system exchange, namely G locus rs4680 gene COMT, G locus rs17851478 and/or in the locus rs1611115 gene DBH, With the locus rs6275 DRD2 gene and identifying at least one allele of the risk of the system determine genetic predisposition to the development of substance dependence.
The new approach will allow to estimate more accurately the level of genetic risk of dependence and more fully describe disease.
Our analysis of literary sources showed that every single polymorphic locus declared genes was allegedly potentially important marker of risk of development of dependence. Therefore we propose to use the data of polymorphic variants of genes of various neurotransmitter systems in the brain, such as: dopamine and serotonin, which is a new approach in predisposition to the development of dependence, and, thus, according to the totality of the signs of compliance grounds of «novelty» and «inventive step».
The technical result of the proposed invention is to increase the accuracy of evaluation of genetic risk of dependence from 73% in the prototype to 81% and, therefore, increasing the opportunities for the most fully characterize disease.
For genotyping of patients, i.e. to identify the G allele in the locus rs1042173 SLC6A4 serotonin system exchange and alleles of genes ' groups of the dopamine system exchange, namely G locus rs4680 gene COMT, G locus rs17851478 and/or in the locus rs1611115 gene DBH, With the locus rs6275 DRD2 gene, using a polymerase chain reaction (PCR) (Polymeropoulos N, Xiao H, Rath D. Merrill C. Tetranucleotide repeat polymorphism at the human tyro-sine hydroxylase gene (TH). Nucleic Acid Res. 1991.19:3753), which DNA fragments that contain polymorphic loci to a sufficient concentration to their separation by electrophoresis.
The research material are samples of DNA of biological objects control individuals and patients with drug addiction and alcoholism. Allocation of genomic DNA is carried out by phenol extraction with some modifications (Strauss WM. Preparation of genomic DNA from mammalian tissue. In "Current Protocols in Molucular Biology (Ausubel FM et al., Eds.) Boston. MA / pp.2.2.1-2.2.3, 1990).
Identify the G allele in the locus rs1042173 SLC6A4 serotonin system exchange and alleles of genes ' groups of the dopamine system exchange, namely G locus rs4680 gene AGR, G locus rs17851478 and/or in the locus rs1611115 gene DBH, With the locus rs6275 DRD2 gene is carried out with the subsequent cutting of the PCR fragments processed by endonucle ases restriction ApoI, FatI, BstENI, FauI and NcoI respectively using electrophoretic separation products (3-4% agarose gel stained bromide . Electrophoresis spend in the buffer TAE 20 minutes at 120 Century Visualization of the obtained results is performed in ultraviolet light (UV).
Determining the accuracy of the method
Were surveyed 1,000 people: 800 patients INTERSECTORIAL narcology, 450 of them drug addicts, 350 patients with alcoholism and 200 healthy people without signs of dependence. Were tested samples of biological objects surveyed using PCR for risk alleles G locus rs1042173 SLC6A4, G rs4680 in the locus of the gene AGR, G locus rs17851478 and/or in the locus rs1611115 gene DBH, With the locus rs6275 DRD2 gene, characterizing the state of the systems of the body that play a key role in the development of dependence.
In patients with simultaneous detection of allele G locus rs1042173 SLC6A4 serotonin system exchange and at least one allele groups of genes of the dopamine system exchange, namely G locus rs4680 gene AGR, G locus rs17851478 and/or in the locus rs1611115 gene DBH; The locus rs6275 DRD2 gene percentage of overlap of the laboratory research with clinical diagnosis was 82%. For healthy people, the percentage of overlap was 91%.
We conducted a study of 200 patients risk alleles in the polymorphic genes complex specified in the prototype neurotransmitter systems: dopamine, serotonin, and acid polymorphic genes SerT, NPY, HTR2C, GAD2, DRD4, , IAIA, DRD2, DBH, DAT the percentage of overlap with clinical diagnosis in patients diseases dependence amounted to 73%.
Example 1
Patient A. addressed in the INTERSECTORIAL narcology advice on the availability of the occurrence of dependence in connection with the fact that his father is sick with alcoholism, and sister abused drugs. The results of medical-genetic study of PCR testified to the presence of the patient's G allele in the locus rs 1042173 SLC6A4 serotonin system exchange and 1 allele groups of genes of the dopamine system exchange, namely G rs4680 gene AGR, which allowed to make a conclusion about the genetic predisposition to dependence. Patient A. recommendations on formation of a healthy way of life.
Example 2
The patient Century asked the INTERSECTORIAL narcology advice on the availability of occurrence of dependence in connection with the fact that her mother is sick with alcoholism and sister abused drugs. The results of medical-genetic study of PCR testified to the presence of the patient G allele in the locus rs1042173 SLC6A4 serotonin system exchange and allele groups of genes of the dopamine system exchange, namely G locus rs17851478 gene DBH, which allowed to deduce the existence of genetic susceptibility to abuse of surfactants.
Example 3
Patient And. addressed in the INTERSECTORIAL narcology advice on the availability of the occurrence of dependence in connection with the fact that his father is sick with alcoholism, and brother abused drugs. The results of medical-genetic study of PCR testified to the presence of the patient's G allele in the locus rs 1042173 SLC6A4 serotonin system exchange and 2 alleles of the genes ' groups of the dopamine system exchange, namely G rs4680 gene AGR, G rs17851478 gene DBH, which allowed to make a conclusion about the genetic predisposition to dependence. Patient I. recommendations on formation of a healthy way of life.
Example 4
Patient C. admitted to the hospital INTERSECTORIAL narcology in a condition of narcotic intoxication. History: the systematic use of heroin for 6 years. Concomitant diseases: chronic hepatitis C. the Results of medical-genetic study of PCR testified to the presence of the patient's G allele in the locus rs1042173 SLC6A4 serotonin system exchange and 3 alleles of the genes ' groups of the dopamine system exchange, namely G locus rs17851478 and locus rs1611115 gene DBH, With the locus rs6275 DRD2 gene, which allowed making conclusion about the coincidence of clinical and laboratory forecast.
Example 5
Patient P. addressed the INTERSECTORIAL narcology advice on the availability of occurrence of dependence in connection with the fact that her mother is sick with alcoholism and cousin is abusing drugs. The results of medical-genetic study of PCR testified to the presence of the patient G allele in the locus rs1042173 SLC6A4 serotonin system exchange and 2 alleles of the genes ' groups of the dopamine system exchange, namely G rs4680 gene AGR and G locus rs17851478 gene DBH, which allowed to deduce the existence of genetic susceptibility to abuse of surfactants.
Example 6
The patient K. addressed the INTERSECTORIAL narcology advice on the availability of occurrence of dependence in connection with the fact that her uncle was sick with drug addiction. The results of medical-genetic study of PCR testified to the presence of the patient 2 alleles only groups of genes of the dopamine system exchange, namely G locus rs17851478 gene DBH and The locus rs6275 DRD2 gene dopamine system, which allowed making conclusion about the absence of genetic susceptibility to abuse of surfactants.
As seen from the above examples, the accuracy of the proposed method is characterized by the percentage of matches with clinical diagnosis in patients dependence diseases, increased compared with the prototype from 73% to 81%.
Way a more comprehensive definition of genetic predisposition to the development of psychoactive substance dependence, including the identification of biological objects of the patient by the method of polymerase chain reaction allele G locus rs1042173 SLC6A4 serotonin system exchange and at least one allele, selected from a group of genes of the dopamine system exchange, namely G locus rs4680 gene AGR, G locus rs17851478 and/or in the locus rs1611115 gene DBH, With the locus rs6275 DRD2 gene and identifying at least one allele of the risk of the system determine the a genetic predisposition to the development of substance dependence.