Method for simulating experimental isolated optic neuritis. RU patent 2504020.
| Method for simulating experimental adenoviral uveitis complicated by optic neuritis / 2504019 Cultural fluid 0.1 ml containing type 6 adenovirus adapted to a pig's finite embryo renal cell line in a dose of 10000 TCD50 is introduced through a flat portion of a ciliary body into a chinchilla's vitreous body with a needle 33 G. That is followed by the analysis of the removed eyeball starting from the 7th post-infection day. |
 Method for experimental finasteride simulation of induction of functional activity of glycoprotein-p / 2504018Invention aims at studying a membership of the analysed drug preparations among efflux carrier protein Pgp (glycoprotein P) substrates. That is ensured by the experimental simulation of the induction of functional activity of the same protein. Finasteride is used as an inducing preparation. The preparation is introduced into rabbits intragastrically in the form of a suspension in olive oil in a daily dose of 0.225 mg/kg of animal's body weight for 14 days. |
 Method for simulating enamel demineralisation centre / 2503067Dental bracket is fixed onto an extracted tooth. A centre surrounding the dental bracket on a vestibular surface is limited by a wax layer. The tooth is immersed into a separated container with a demineralising gel consisting of (wt %): calcium dihydrophosphate - 0.04-0.08, lactic acid - 0.8-1.0, praestol 2510 - 3.0-4.5, sodium hydroxide - 0.4, distilled water - the rest. Then, the container with the teeth is placed in a thermostat at pH=4.5 for 96 hours. |
 Method of simulating atherosclerosis / 2500041For the purpose of diagnosing, preventing and treating the same disease, a cholesterol powder in the amount of 1%, margarin 10%, mercazolilum 10 mg/kg and vitamin D 2.5 IU per kg of body weight is added to the laboratory rats' feed. That is combined with performing an operation of ligating a left renal pedicle with a non-absorbable suture and underrunning a right upper pole with 2/3 of the organ being preserved. |
| Method for combined tadalafil and l-norvaline correction of l-name induced nitrogen oxide deficiency in experiment / 2500040 Nitrogen oxide deficiency is simulated by the daily 7-day intraperitoneal introduction of N-nitro-L-arginine methyl ester 25 mg/kg into experimental Wistar male rats. The nitrogen oxide deficiency is corrected by the simultaneous 7-day intragastric introduction of a combination of tadalafil 0.09 mg/kg and L-norvaline 10 mg/kg once a day. |
| Method for simulating primary biliary cirrhosis / 2500039 Primary biliary cirrhosis is simulated by introducing 45-50% alcoholic solution of picryl sulfonic acid 0.08-0.12 ml into rat's bulbar duodenal lumen and terminal ileum every 5-10 minutes. The primary biliary cirrhosis is diagnosed 1-1.5 months later. |
| Method for simulating chronic infected bone wound / 2499295 Bone defect is formed along the bone; the museum strain Staphylococcus Aureus No.5 culture in the amount of 40-45 mln CFU per 1 kg of experimental animal's body weight mixed with 0.1 ml of sterile silica sand are placed therein. Three days later, necrotic mass and purulent matter are removed from the bone defect. The wound is re-infected with the same culture in a dose of 20-25 mln CFU per 1 kg of body weight. The wound is further preserved in the form of a fistula. |
| Method for simulating diabetic macular oedema / 2498415 Alloxan is introduced into rat's peritoneum in a dose of 15.0 mg/100 g of weight. Then, 6.5 weeks after alloxan has been introduced, and experimental diabetes has been developed, insulin-like growth factor 1 (IGF-1) 1 mcl is introduced into the vitreous body from an intravitreous approach. |
| Method for dermal flap survival growth in reduced circulation with sildenafil / 2498414 Dermal flap is simulated in laboratory animals on the second day of experiment. Sildenafil 2.2 mg/kg is introduced intraperitoneally on the first, third and fifth day of the experiment. |
| Method of diagnosing affection of peripheral nerves in laboratory animals in remote period of exposure to mercuric chloride / 2497448 Invention relates to field of medicine, in particular to experimental medicine. Stimulatory myography is performed to laboratory animals 9 weeks after exposure to toxicant is stopped. Amplitude of M-response (mV) and duration of M-response (ms) on median nerve are registered. Area of involvement of motor units is calculated. Calculation of canonic value is performed. Obtained results are compared with constant and if Cv is higher than 8.9, conclusion about absence of signs of chronic exposure to mercuric chloride is made, if Cv is lower or equals 8.9, affection of peripheral nerves in remote period of exposure to mercuric chloride is diagnosed. |
 Method for modeling acute pancreatitis / 2244345As experimental animals one should apply mongrel dogs of 12-17 kg body weight. Under general anesthesia one should conduct superior-median laparotomy, introduce 3.0 ml 70%-ethanol solution under pancreatic capsule and then laparotomic wound should be sutured up. Manipulation should be performed once. The method provides modeling adequate acute pancreatic inflammation at no side effects being very simple in implementation. |
 Method and device for studying transosseous osteosynthesis model stiffness / 2246139Method involves studying transverse longitudinal and rotation stiffness characteristics. The studies are carried out step-by-step from the first order units to complete external fixation apparatus structure. The device has frame and is provided with calibration loads, wire rope, displacement indicators, strip for fastening to loading end of bone imitator fragment, beam for fixing displacement indicators, beams having unit for modeling longitudinal and transverse loadings. The frame is manufactured as parallelepiped. The fixing panel has openings for bone imitator, for fixing external fixation apparatus and yoke connection union and is fixed in end face part of the frame. Beam for fixing displacement indicators has longitudinal slit for fixing the indicators and arranging them on lateral slots in frame base. The beams having unit for modeling rotational, longitudinal and transverse loadings are arranged on lateral frame sides on lateral slots in base. |
 Method for experimental modeling urinary calculosis / 2248045The present innovation deals with modeling urinary calculosis in rats due to injecting intraperitoneally 60%-glucose solution at 1 ml/100 g animal body weight twice daily for 2 mo. The method is very simple and enables to achieve lithogenesis in 25% experimental animals. |
| Method for applying wave action to pathogenic microorganisms, viruses and tumor cells in experiment / 2250788 Method involves using Hann diode crystal with proper frequencies of pathogenic microorganisms and cells during their death period or during the stimulating factors action period being applied. |
 Method for modeling hypoxia with hypercapnia in animals / 2251158Hypoxia with hypercapnia should be modeled due to creating a closed system of inhaled air circulation. Air enters lungs out of hermetically sealed reservoir and at expiration returns back. The process of recirculation is supplied with an apparatus of artificial pulmonary ventilation. The innovation suggested provides steadiness in development of hypoxia with hypercapnia excluding the development of stressor reaction. Conditions should be created to carry out any manipulations with an animal in the course of an experiment. |
| Method for obtaining parodontitis model / 2252009 The present innovation should be carried out for the purpose to study ethiology and pathogenesis of parodontitis. One should affect with emotional stress in experimental animals (mature rats) due to placing 10-11 experimental animals into the cage at area of 0.018 sq. cm/animal. Before placing into the cage one should create artificial dental plaque around the cervix of the upper and lower incisors with the help of stomatological cement for every experimental animal. In the course of modeling all experimental animals should eat paste-like food. The method enables to shorten terms for obtaining the model desired and increase its similarity with pathomorphological manifestations of human parodontitis. |
 Acupuncture point electric model device / 2252743Device has input first variable resistor, capacitor and permanent resistor. Permanent resistor is connected to arm second and third variable resistors. Second ends of variable resistors are connected with motionless contacts of polarized relay. Movable contact of relay is connected to common bus. Input of device is connected to amplifier which has output connected with control wiring of polarized relay. Second end of wiring is connected with common bus. Device is intended for electrical modeling of balanced and misbalanced conditions of acupuncture point at electropunctural action with unlike-poled signals due to liquidation mutual errors at any circuit of opposite arms of the device. Values of active resistances can be installed independently at any arm of device. |
| Method for modeling hypoxic encephalopathy / 2253152 Laboratory animals should be once injected intraperitoneally or intravenously with phenylhydrazine at the dosage of 100-150 mg/kg. |
| Method for modeling chronic toxic nephropathy / 2253153 At studying the mechanisms of heavy metals toxic action, in particular, cadmium upon renal function, it is suggested to introduce cadmium sulfate solution into stomach once daily for 2 mo at the dosage of 0.5 mg/kg, on conversion to metal, where cadmium corresponds to 0.5 mg per 1 ml solution. The present innovation enables to study the pathology in dynamics of development and elaborate and searching preparations for treating and preventing chronic toxic nephropathy. |
| Method for stopping carcinoma cells division in a biological object / 2253903 Method involves exposing cell or cell group to external power source. At least two electrodes are introduced before treating the cells. One of electrodes is set on cytoplasmatic external cell membrane surface and the other one cell membrane and membrane potential value is measured. External electric voltage source is connected to the introduced electrodes oppositely in polarity with cell membrane potential difference value being not less than cell membrane potential. |
FIELD: medicine.
SUBSTANCE: cultural fluid 0.1 ml containing the strain L2 of type I herpes simplex virus adapted to a pig's finite embryo renal cell line in a dose of 100000 TCD50 is introduced through a flat portion of a ciliary body into a chinchilla's vitreous body with a needle 33 G. That is followed by the histological analysis of the removed eyeball starting from the 21st post-infection day.
EFFECT: higher rate and accuracy of the reproduction of isolated optic neuritis.
1 ex
Invention refers to medicine, in particular to ophthalmology, and can be used to create models of the disease in experimental ophthalmology.
With the introduction of herpes simplex virus in front of the camera eye rabbit mainly develops experimental herpes keratouveitis (70-90% of the animals), rarely pigmentosa, the defeat of the optic nerve were observed (V.B. . Ophthalmic herpes: clinical picture, diagnosis, treatment, Ufa, 1994).
With the introduction of herpes simplex virus in front of the camera white rats, also observed the herpes without hitting the optic nerve, and rarely in the mesh shell revealed , zone - exudate (ibid.).
There is a method of modeling of herpes infection of the eye with the introduction of herpes simplex virus in the vitreous body outbred white mice, however, in this case 100% of the cases were amazed by all the eyeball except the optic nerve (ibid.).
There is a method of modeling experimental optic neuritis in rabbits, comprising an introduction into the orbit of 1.0 ml of 10% solution of alcohol in the background chlorpromazine- narcosis. According to the authors, this concentration can cause clinically visible damage to the optic nerve without the expressed General toxic action of [AD Temirov, E.A. Nesterov, S.. Panchenko (Rostov, Russia) Morphological characteristics of experimental optic neuritis].
The closest analogue of the invention is a method of modelling of optic neuritis by infecting the rabbit cornea herpes simplex virus type I, with histological and virological assays to detect and defeat the optic nerve at the experimental herpetic keratitis (LN. Tarasova, Cytological and histological studies at the herpetic eye disease // abstract. Diss. ... Cand. honey. Sciences. Ashgabat, 1968). However, with this method in 100% of cases damage the cornea in the form of dendritic keratitis and significantly rarely optic nerve is damaged.
When modeling herpes infection of the eye of experimental animals, the smaller the size of the eyeball, the more often develops traumatic injury of the shells of the eyeball. Moreover, in all experimental studies for the introduction of herpes simplex virus used insulin needle large size (at least 22G), which injure the eye and cause nonspecific inflammatory response.
In addition, during the simulation herpes infections of the eyeball herpes simplex pre- on the primary- culture of cells of a human embryo fibroblasts, endothelial cell culture Vero or by intracerebral passages in mice. This led to high pathogenicity of the virus and as a consequence - the defeat of all of the shells of the eyeball and 100% mortality of experimental animals.
Object of the present invention is to create a model of an isolated herpes optic neuritis with high frequency playback closest to the specified disease in humans, when less chance of injury to the eye membranes needle and the death of animals from herpes encephalitis.
Technical result in the use of the invention is increasing the frequency and accuracy of modeling isolated optic neuritis, prevention of unwanted concomitant illness and death of experimental animals.
The proposed method of modeling isolated optic neuritis is as follows. Needle 33 G rabbits of chinchilla breed in the vitreous body of the eye through a flat part of the ciliary body introduce the culture fluid containing the virus herpes simplex virus (HSV) type 1 strain L 2 , adapted to the endothelial lines of embryonic pig kidney cells in a dose of 100000 50 . Spend histological studies of remote eyeball, beginning with the 21st day after infection, which allow the study of the course of the disease.
Eleven rabbits (22 eyes) of the chinchilla breed through the flat part of the ciliary body, vitreous both eyes needle 33 G was administered 0.1 ml of cultural liquid, contains 100000 50 HSV type 1 strain L 2 , adapted to the endothelial lines of embryonic pig kidney cells, obtained by the standard technique [Virology. Methods: Per. from English. / Under. Ed. by B. . - M: Mir, 1988. - .270-287.]. On the 21 day produced the slaughter of animals and histological studies conducted remote eyeballs. In the thickness of the optic nerve, around vessels, discovered extensive hearth destruction of nerve tissue with , dilated vessels, . cloth soaked protein liquid admixture of a large number of neutrophilic leukocytes, eosinophils, monocytes and lymphocytes. In the tissue of the optic nerve, around the hearth of destruction, determined swelling, vessels full-blooded. In the hearth of the destruction of the nervous tissue is visible expressed inflammatory infiltration, provided by a large number of neutrophilic leukocytes, eosinophils, monocytes, lymphocytes. In stroma seen swelling, vessels are stretched, the walls of their thickened by soaking around accumulation edematous liquid soaked by neutrophils, monocytes, . The small vessels of the full-blooded, some of them with the phenomena of stasis, in the walls of the phenomenon impregnation. In areas of the optic nerve, adjacent to vitreous body, a hotbed of destruction, around which determined inflammatory infiltration, dystrophic processes in and marked swelling.
The morphological studies have not revealed the characteristic of herpes infection, inflammatory changes in the other parts of the eyeball, needle damage the lens and the retina. Rabbits died from encephalitis.
Example. Rabbit №2 of the chinchilla breed weight 2,3 kg through a flat part of the ciliary body, vitreous both eyes needle 33 G was administered 0.1 ml of cultural liquid, contains 100000 50 HSV type 1 strain L 2 , adapted to the endothelial lines of embryonic pig kidney cells. On the 21 day produced slaughter the animal and histological studies conducted remote eyeballs. In the thickness of the optic nerve, around vessels, discovered extensive hearth destruction of nerve tissue with , dilated vessels, . cloth soaked protein liquid admixture of a large number of neutrophilic leukocytes, eosinophils, monocytes and lymphocytes. In the tissue of the optic nerve, around the hearth of destruction, determined swelling, vessels full-blooded. In the hearth of the destruction of the nervous tissue is visible expressed inflammatory infiltration, provided by a large number of neutrophilic leukocytes, eosinophils, monocytes, lymphocytes. In stroma swelling, vessels are stretched, the walls of their thickened by soaking around accumulation edematous liquid soaked by neutrophils, monocytes, . The small vessels of the full-blooded, some of them with the phenomena of stasis, in the walls of the phenomenon impregnation. Around the hearth destruction of the optic nerve was determined inflammatory infiltration, dystrophic processes in and marked swelling.
Thus, the proposed method of modeling isolated herpes optic neuritis allows you to create a model closest to the disease in humans. Furthermore, there is no death of experimental animals from encephalitis and injury membranes of the eyeball and the lens, the involvement in the inflammatory process of the other departments eyes.
A method for simulating stand-alone herpes optic neuritis, which is characterized by the fact that through the flat part of the ciliary body of the vitreous body of the eye rabbit breed chinchilla needle 33 G impose 0.1 ml of cultural liquid containing herpes simplex virus (HSV) type I strain L 2
adapted to the endothelial lines of embryonic pig kidney cells in a dose of 100000 50 , in doing so, a histological study of the remote eyeball on the 21st day after infection.