Method for modeling acute pancreatitis. RU patent 2244345.
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FIELD: medicine, experimental abdominal surgery.
SUBSTANCE: as experimental animals one should apply mongrel dogs of 12-17 kg body weight. Under general anesthesia one should conduct superior-median laparotomy, introduce 3.0 ml 70%-ethanol solution under pancreatic capsule and then laparotomic wound should be sutured up. Manipulation should be performed once. The method provides modeling adequate acute pancreatic inflammation at no side effects being very simple in implementation.
EFFECT: higher efficiency.
3 dwg
The present invention relates to medicine, namely to abdominal (experimental) surgery, and can be used to study how the disease of acute pancreatitis and its complications.
Known methods of modeling of acute pancreatitis using dogs as experimental animals. Such methods are methods of Dalgat, and co - introduction autorace under pressure in the left share of the pancreatic duct (Dmmagic, Mstime, Rteditor /Diagnosis and treatment of acute pancreatitis/ journal of surgery. -1986 - No. 4 - p.29-33), the method Amirkhanova and co - ligation of the splenic or pancreatoduodenal arteries (Saelios, Approxichemical, Lavcevic /Guidance on experimental surgery. - Moscow: Medicine - 1989), methods Lngelheim and Liedekerke - introduction into the tissue of the pancreas pancreaticoduodenal serum in combination with conditionally pathogenic microorganisms (Saelios, Approxichemical, Lavcevic /Guidance on experimental surgery. - Moscow: Medicine - 1989). All the above works proved the development of acute pancreatitis. However, despite the validity of the results, the authors state the complexity of the data manipulation, and in some cases high mortality in the postoperative period.
The prototype of the present invention is a method of modeling of acute pancreatitis local effects of damaging factors (CEC, Casimiroa, Vmmandy, Laskowski. /The effectiveness of combined use of electrophoresis of 5-fluorouracil and magnetic therapy in experimental pancreatitis. /Balneology and physiotherapy - 1994 - No. 3 - p.17) by cooling the tissue of the pancreas irrigation-chloroethyl. The disadvantage of this method is the possible impact of harmful factors on the surrounding tissue and organs and thereby the occurrence of side effects.
The task of the invention is to develop a reliable, technically easy to perform a method of modeling of acute pancreatitis.
The technical result in the use of the invention is to simplify the way, preventing side effects.
The method of modeling of acute pancreatitis is as follows. As an experimental animal use mongrel dogs weighing 12-17 kg Under General anesthesia produce verhnesadovoe laparotomy, under the capsule of the pancreas in the body is injected 3.0 ml of 70% ethanol, then laparotomic wound is sutured. Manipulation produced once.
To assess the validity of the results of the proposed method of modeling of acute pancreatitis was performed histological examination of tissue slices of the pancreas. The method was tested on 15 dogs. On the 7th day after the operation was performed procedure euthanasia of experimental animals by intracardiac injection of 100.0 ml of air (the procedure was performed under General anesthesia), then produced a biopsy of the pancreas. After the corresponding histological posting a slice thickness of 7 μm was colored with hematoxylin-eosin. Just cooked 180 specimens.
Figure 1 shows the destruction of the secretory departments of the pancreas in experimental animals (coloring hematoxylin - eosin, microfoto, eyepiece 10, a lens 20), figure 2 shows the congestion and perivascular edema with infiltration of lymphocytes in the pancreas of experimental animals (coloring hematoxylin-eosin, microfoto, eyepiece 10, the lens 40), figure 3 shows large accumulations of lymphocytes in Magdalinovka connective tissue of the pancreas in experimental animals (coloring hematoxylin-eosin, microfoto, eyepiece 10, the lens 40).
In histological preparations of the pancreas in experimental animals are identified extensive areas with pronounced destructive processes (Fig 1.). Cell secretory Department reduced in size, they are polygonal in shape, homogeneous and Imogene zones are not defined, their nuclei also sealed or single cells devoid of nuclei. The cytoplasm of many of them is colored intensely basophile, while others in the form of light bubbles. Degenerative processes are observed in the islet apparatus. Many insularity reduced in size, their nuclei with signs of karyopyknosis and even karyolysis. The capillary network is poorly manifested. However, in remote areas small slices have a normal histological structure, when the secretory departments are functional activity, i.e. the homogeneous part of the secretory cells are basophilia, cell nuclei are round or oval in shape, whereas in the apical part of the cytoplasm of the cells are determined secretory granules. The network of blood capillaries without significant changes. In the area of experimental exposure to blood vessels with severe congestive phenomena (figure 2.). Perivascular edema is accompanied by the migration of lymphocytes, macrophages and plasma cells. Meet simultaneously and large accumulations of lymphocytes, often in Magdalinovka connective tissue (figure 3), and the cells of lymphoid tissue are tight. Nearby blood vessels are also characterized by the plethora and congestion.
Thus, in the pancreas of experimental animals shall be expressed destructive and degenerative processes. Especially in the area of chemical factor in the acini of the glands shrink, acidify no signs of secretory activity. Degenerative processes cover not only the cytoplasm and nuclei of secretory cells. Along with the destructive processes of the exocrine part of the gland is determined by circulatory disorders of the endocrine part of the pancreas. Midorikawa connective tissue characterized by infiltration of lymphoid tissue, as well as congestion in the major blood vessels.
Collectively, all stages of the inflammatory process (acute pancreatitis).
The method of modeling of acute pancreatitis by local effects of the damaging factor, characterized in that once imposed 3.0 ml of 70% solution of ethyl alcohol under the capsule of the pancreas in the body.