Method for applying wave action to pathogenic microorganisms, viruses and tumor cells in experiment

FIELD: medicine.

SUBSTANCE: method involves using Hann diode crystal with proper frequencies of pathogenic microorganisms and cells during their death period or during the stimulating factors action period being applied.

EFFECT: enhanced effectiveness of treatment; wider range of biophysical action types.

3 cl, 1 tbl

 

The invention relates to information-wave medicine and medical technology and can be used in physiotherapy, reflexology and bioresonance therapy to regulate the functional activity of biological systems.

There is a method of treating a pathological state of the organism biologically active fluid (BAJ) using chemically and electrically neutral water, exposed to electromagnetic fields (special technique), by application of medical information individually selected test objects (VNIRO. Guide electropunctural diagnostics and drug-free treatment BAJ. - Alma-ATA, 1991. - p.27-37).

However, this method is not effective, due to the fact that the Belarusian Association of journalists as media unstable and requires sophisticated technology of storage and use.

There is a method of treatment of pathological conditions of the body by measuring biopotentials in biologically active points before and after removing the electromagnetic wave characteristics with matrix medical test objects, then determine using a controlled pulse laser frequency-wave parameters Meridian psyche with the transfer of information on solid media, which is used in its continuous contact influence on measures of the Dian psyche (Patent of the Republic of Kazakhstan No. 332, publ. 10.06.96).

However, this method has low effectiveness of physiotherapy in the treatment process due to the low specificity bioinformatics effects on the body.

There is a method of treatment of pathological conditions, including electro-acupuncture testing haloaliphatic drugs, natural vibrations of the body of the patient or their frequency-wave analogues followed by resonant transfer of information to the media with the possibility of changing the phase angle of 180°. As the carrier using a crystal diode Gunn. The transfer of information is carried out by feeding the supply voltage in direct contact with drugs and then off. After that, the Gunn diode is placed on biologically active points and zones of the body (Patent RF № 2141304, IPC And 61 N 39/00, publ. 20.11.99).

However, this method also has a low effectiveness of physiotherapy in the treatment process due to the low specificity bioinformatics effects on the body.

The closest technical solution (prototype) is the way bioinformatics effect on microorganisms, including medical record wave of information about their own variations of the microorganism and information about Romeo and allopathic medicines nancial, made in the form of a crystal diode Gunn, and its subsequent placement on biologically active points and zones of the body. Crystal Gunn diode is made in the form of a structure with separated working volumes and record information about each drug is carried out on the respective working volume of the crystal diode Gunn by feeding the supply voltage to the working volume by direct contact of the crystal with the drug and its subsequent off (Patent RF № 2155083, IPC And 61 N 5/00, publ. 27.08.2000).

However, this method also has a low effectiveness of physiotherapy in the treatment process due to the low specificity bioinformatics effects on the body.

The technical result of the invention is to increase the specificity of bioinformatics effects on the body due to the use as a therapeutic wave information the information recorded on the media in the process of destruction of pathogenic microorganisms, or viruses, or tumor cells, or the information recorded on the media in the process of intensive growth of beneficial autochthonous microflora.

This technical result is achieved in that in the method of bioinformatics effect on microorganisms, or viruses, or tumor cells, including medical record wave information from the containing a series of information about one's own electromagnetic oscillations of the microorganism, or virus, or tumor cells, and wave information from an object with therapeutic properties, to the media, made in the form of a crystal diode Gunn, by feeding the supply voltage to the working volume of the crystal diode in direct contact with the microorganism or virus, or tumor cells, or an object with therapeutic properties, and then off, and place the crystal on biologically active points and zones of the body, according to the invention as an object with therapeutic properties, use their own variations of pathogenic microorganism or virus, or tumor cells in the period of their death when exposed to any abscopal factors or vibrations autochthonous microorganisms in the period of exposure to favorable incentives, providing intensive development and reproduction of autochthonous microorganisms in the logarithmic growth phase. As an adverse factor causing the death of a pathogenic microorganism or virus, or tumor cells, use of antibiotics, or sterilizing agents, or exposure to heat or ultraviolet or radiation exposure.

As appropriate enabling factors for intensive development and reproduction auto is ton of microorganisms in the logarithmic growth phase, are bifidogenic, lactogenic, growth factors, vitamins and trace elements.

The recording time of healing wave information from the object, with a therapeutic effect on the media is 10-40 minutes.

The method is as follows. First by electro-acupuncture diagnostics electrical condition of biologically active points of the body of the patient, for example, the device type "Medeo" before and after drug test objects that have medicinal properties, and natural vibrations of the body.

For implementing the method using a portable handheld device with a set of emitters is extremely high purity (EHF) frequency range (42,2-100,0) GHz for EHF-therapy of type "Stella" with media set-color resonant microwave information (crystal Gunn diode), developed by the inventor A.M. Andriy Kozhemiakin and protected by the RF patent № 2141304, IPC And 61 N 39/00, publ. 20.11.99. The medium contains a polymer insulating sheath, a Gunn diode, made in the form of a semiconductor crystal with metal contacts and the conductors of supply.

For use in therapeutic purposes write on crystal healing wave information, containing information about their own variations of the patient's body, on its own fluctuations of the microorganism, VIR is sa or tumor cells, as well as information about their own variations of these objects during their death when exposed to inhibitory factors or information about their own vibrations autochthonous microorganisms of the patient during the period of exposure to favorable incentives, providing intensive development and reproduction of autochthonous microorganisms in the logarithmic growth phase. Device (media) placed near a source of healing wave information on the Gunn diode through the conductors serves the supply voltage specified in the passport (3 volts). This changes quantummechanically parameters of the crystalline structure of the diode and to the emission of electromagnetic energy in the microwave range. The radiated wave from the diode goes to the object, with medicinal properties, modulates its own oscillations, paratragedy and comes to the diode. When switching off the supply voltage of the diode its crystal structure is returned in the original quantummechanical state, which is influenced by healing wave information received by the crystal with the reflected electromagnetic wave, and the media (Gunn diode) is ready to use. Further work is media based on the conversion of the energy of thermal vibrations of the crystal d is etki semiconductor and external fields in the low-intensity energy wave radiation, where as a part of the present therapeutic vibrations, and the device is without a supply of external power. To eliminate interference with the recorded parameters must submit to the supply voltage. Overwrite healing wave information can be done multiple times.

To ensure the patient's crystal Gunn diode is placed in a biologically active points and zones of his body, fixed media, for example, patch, where it has a long contact therapeutic effect on the body.

Below are examples (1-5) preparation of media, with medicinal properties, and experimental studies bioinformatics impact on biological objects.

Example 1. The way bioinformatics impact on the human immunodeficiency virus

Experimental studies show that ethanol at a concentration of 5%, 2.5% and 1.25% of no toxic effects on human cells, in particular cells MT-4, but significantly inhibit the infectious activity of the human immunodeficiency virus.

The studies were conducted in HIV-1 strain by incubating equal volumes of non-toxic final concentration of 5%ethanol (inhibitory factor) and the initial concentration vaccinated suspension of HIV-1 at room themes is the temperature value for at least 30 minutes. After 10 minutes near investigated (containing ethanol) and control (does not contain ethanol) samples of vaccinated place liquid capsules with native speakers, made in the form of a Gunn diode, connected to the apparatus type “Stella”, and incubated for 10-20 minutes.

The media with the recorded medical information used in the following way. Prepared samples of HIV-containing suspension, part of which was processed by placing around them capsules with native medical information about the death of HIV infection in 12-24 hours. Control samples from vaccinated suspension is not subjected to bioinformatics impact.

After that prepare a tenfold dilution vaccinated suspension study and control samples in culture medium RPMI-1640 with the addition of 10% serum fetal cow, 2 mm L-glutamine, 100 μg/ml kanamycin, 160 μg/ml gentamicin, making permissively lymphoblastoid cell culture human MT-4, scattered in 96-well culture tablets company "Costar" in a volume of 200 µl with a concentration of 1 million cells/ml Cell suspension incubated at 37 ° C in a humid atmosphere (95%) with 5% CO2and titration of infectivity of HIV-1 in culture human lymphoblastoid cells MT-4. Monitoring is conducted daily what UPE by microscopy of wells under an inverted microscope (Diavert" registration process of cluster formation, the viability of cells and preparation of slides of cells on a slide glass with non-fatty determine the number of infected cells MT-4 in the reaction of indirect immunofluorescence assay (NIF). Smears of cells after drying locks in the solution chilled to minus 20 degrees With acetone for 12 hours and Then process them working dilutions of sera containing specific antibodies to HIV-1 (1:100) and control for 30 min in a humid chamber at a temperature of 37 ° C. then washed three times in saline solution and dyed rabbit antibodies against human immunoglobulins labeled with FITZ, in a dilution of 1:32 for 20 min in a humid chamber at a temperature of 37 degrees C. At the final stage strokes twice washed with buffered saline solution and rinsed with distilled water after which spend their microscopy, the fluorescent microscope (Axioskop" firm Opton (Germany), determining the percentage of HIV-1-infected cells MT-4 and the intensity of the fluorescent glow of infected cells.

At 12-24 hour bioinformatics processing samples of HIV-containing cell suspension MT-4 medical information on the death of the virus, recorded on a carrier, there is a significant change in the infectious properties of the virus, because the clustering of infection is different cells MT-4 was maintained in all the tested dilutions of the virus at the level of uninfected control cells. In used doses of medical information completely inhibits the infectivity of HIV-1 to 2 lg. This significantly decreases the intensity of the fluorescent glow of infected cells MT-4, and hence the degree of reproduction of human immunodeficiency virus 1 type in them. In the control samples infectious activity of HIV-1 is preserved.

Example 2. The way a bioinformatics effect on tumor cells of Ehrlich adenocarcinoma

For the experiment, prepare tubes with samples of cell suspension transplantable tumors adenocarcinoma Ehrlich in the amount of 20 ml each. Some of the specimens are placed in an incubator with a temperature of 42.5° (inhibitory factor) and incubated at the same temperature for 50 minutes. 15 minutes after the start of the experiment in a test tube is placed in the capsule with the media, made in the form of a Gunn diode, connected to the apparatus type “Stella”, which is recording information about the death of tumor cells. After 50 minutes, the tubes are removed from thermostat. Next part of the test tubes with a suspension of tumor cells that were not exposed to heat, place the capsule with the recorded medical information about cell death transplantable tumors adenocarcinoma Ehrlich and maintain them within 24 hours.

Further experiments about the W ill result in outbred ICR mice, the males weighing 25-30 g, which transplantation of the patient subcutaneously in the region of the armpit at a dose of 105 tumor cells in an animal: one group was injected control untreated suspension of tumor cells; the second group - the suspension of tumor cells treated at a temperature of 42.5°and the third group of mice - a suspension of tumor cells subjected to bioinformatics effect within 24 hours. After a few days in the first group of mice showed marked growth of tumor tissue, and the second and third group within 10 days of the observation of the formation and growth of tumor tissue was not found.

Then took the first group of mice with a distinct adenocarcinoma Ehrlich and each mouse daily during the week, recorded on the area of the tumor capsule from the carrier medical information about the death of tumor cells. After a 7-day bioinformatics effects in 100% of mice observed marked growth inhibition of the tumor site.

Example 3. The way bioinformatics impact on vegetative forms of bacteria

To obtain vegetative forms of bacteria take several samples of 1 g dry preparation of Bacillus subtilis type “Baktisubtil” in spore form, each dissolved in 10 ml of saline solution at a temperature of 85°C and maintained at this temperature for 30 minutes. While str is a new form of bacteria enters the vegetative. Then 1 ml suspension of bacteria are placed in Petri dishes on the surface of the agar with mesopartner broth and incubated for 36 hours in a thermostat at a temperature of 37°C. Then loop these bacteria in the vegetative form is transferred into several test tubes with mesopartner broth, which is injected in 2 ml of penicillin activity of 1,000,000 UNITS (inhibitory factor), stirred and placed in a thermostat at a temperature of 37°C. After 10 minutes in a test tube with bacteria enter a sterile set of capsules with native speakers, made in the form of a Gunn diode, connected to the device type “Stella”, which is a record of medical information on the death of the bacteria in the vegetative form. Capsules information removed from the tubes through 10-20 minutes.

For experimental validation of bioinformatic the effects of media on bacterial vegetative cells form the control and test samples. To do this, take 1 ml suspension of bacteria in Bacillus subtilis vegetative form and placed in Petri dishes on the surface of the agar with mesopartner broth and incubated for 36 hours in a thermostat at a temperature of 37°C. near the investigated samples are placed in the capsule with the media about the death of bacteria in the vegetative form. Analysis of samples after incubation shows that in control samples observed the Xia active growth of bacterial colonies and in the test - delay their growth.

Example 4. The way bioinformatics impact on spore forms of bacteria

Take several samples of 1 g dry preparation of Bacillus subtilis type “Baktisubtil” in spore form, each dissolved in 10 ml of 1%formalin solution at a temperature of 70-85°C and maintained at this temperature for 50 minutes. 20 minutes after the start of the next experiment with the samples placed or injected into the tubes with bacteria sterile capsules with native speakers, made in the form of a Gunn diode, connected to the apparatus type “Stella”, which is a record of medical information on the death of the bacteria in spore form. Capsules information sequentially removed from the test tubes 15-30 minutes.

Next, take the tubes with disputes 1 g dry preparation of Bacillus subtilis. Some of them leave as a control, and in the other place the capsules with the media about the death of bacteria in spore form and maintain them within 12-24 hours at room temperature. Then in a test tube with the control and intervention samples injected 10 ml of saline solution with a temperature of 85°C and maintained at this temperature for 30 minutes. When this spore form of the bacteria enters the vegetative. Then 1 ml suspension of bacteria from each tube was placed in Petri dishes on the surface of the agar with meats is peptone broth and incubated for 36 hours in a thermostat at a temperature of 37° C.

Analysis of samples after incubation shows that in the control samples, there is active growth of colonies of bacteria, and in the test - delay their growth.

Example 5. The way bioinformatics effects on autochthonous microflora of mammals

First, take samples of faeces from a group of laboratory rabbits participating in the experiment. The investigated material is titrated with a sterile saline solution, making serially tenfold diluted to 108. From different breeding specified material inoculated on nutrient medium, Blaurock, aloes, Endo, Viburnum, Mrs-4, blood agar with polymyxin to determine the baseline levels of coliform bacteria, lactobacilli, bifidobacteria, enterococci, staphylococci and other bacteria with subsequent identification and selection of autostartup normal intestinal microflora containing bifidobacteria and lactobacilli, coliforms, lactic acid streptococci, lactobacilli bacteria. Two days later material, for example, from isolated colonies of lactobacilli grown in the medium Mrs-4, isolated from colonies of bacteria growing on the environment Blaurock from isolated colonies of enterococci, e.g. Streptococcus faecium, grown on medium Kalina, from isolated colonies of Escherichia coli (E. coli)grown on blood agar with polymyxin, t see you a again is subcultured onto appropriate selective medium. Grew up two days after the second passage of the isolated colonies of bifidobacteria and lactobacilli, enterococci, Escherichia coli subcultured (separately) by the above nutrient medium, Blaurock, Mrs-4, Kalina, blood agar with polymyxin and cultured by standard methods to obtain the biomass of microorganisms. For example, the biomass of bifidobacteria has a titer of not less than 109 cells/ml, biomass lactobacilli - 109-1010 cells/ml, biomass Escherichia coli - not less than 107-CL/ml, and biomass Streptococcus faecium is not less than 109 cells/ml Then the biomass of each species autochthonous strains of microorganisms administered in equal ratio to the reactor and stirred to obtain a mixture of bacteria. As the mixture is injected differentiated nutrient medium on the basis of, for example, corn hydrolysate with added stimulating bifidogenic, lactogenic and growth factors: insulin, sodium Selenite, vitamins, amino acids and cultivated biomass at a temperature of 37°C to logarithmic phase of growth (no more than 4-5 hours). Next to the reactor enter a sterile set of capsules with native speakers, made in the form of a Gunn diode, connected to the apparatus type “Stella”, which is recording information about the intensive growth and development of the autochthonous microflora. Capsules incubated for 15-30 minutes, turn off the device “Stella” and Animat from the reactor.

Then the rabbits injected with antibiotics for 10 days to obtain explicit dysbacteriosis. Next, rabbits divided into three groups. The first is a control group, which only provide the food. The second group treatment probiotics such as bifidobakterii and colibacterin, which is introduced into animal feed, for 10 days. The third group of animals is attached in the region of the gastrointestinal tract (in different departments) capsules with native bio-information about the intensive growth and development of their autochthonous microflora, which are not removed within 7 days.

Table 1

Bacteriological studies of the intestinal microflora rabbits after treatment
The name of the microorganismsThe number of microbes in 1 g of feces of mice
The 1st group (control)2nd group of rabbits after treatment with probioticsGroup 3 rabbits after a bioinformatics impact
1. Lactobacillus105108108-1010
2. Bifidobacterium105109108-1010
3. Bacteroides105-106106 -108108-1010
4. Escherichia (lac+)105-106105-108109
5. Escherichia (Lac-)105-106105-106-
6. Escherichia (laborer.)105-106105-106-
7.Escherichia(Gamal.)105-106105-107-
8. Enterococcus105105-lO7105-109
9. Staphlococcus105-106--
10. Proteus105-106--
11. Clostridium105-106102-
12. Fungi Candida105-108103-104-

Bacteriological studies fekalii three groups of rabbits was conducted in accordance with guidelines of "Dysbiosis and methods of laboratory diagnostics", which showed that treatment with probiotics and bioinformatics impact on ICRI the flora of animals was not only a significant reduction in the level of pathogenic and conditionally pathogenic microflora, but the increase in bifidobacteria and lactobacilli almost equally from 105 to 108-1010 microbial cells in 1 g of feces (table 1), the displacement of some of conditionally pathogenic microorganisms.

Thus, the present invention can be used for the treatment of pathological conditions caused by various pathogenic microorganisms, viruses, and prophylaxis and treatment of oncological diseases and dysbacteriosis with high specificity without the use of drugs or greatly reduce their dose and side effects.

1. The method of wave action on pathogenic microorganisms, viruses or tumor cells in the experiment using a crystal diode Gunn by feeding the supply voltage to the working volume of the crystal with prior contact with such a microorganism or virus, or tumor cells with subsequent disconnection of the crystal and placing it on biologically active points and zones of a mammal, characterized in that prior contact with crystal use their own variations of pathogenic microorganism or virus, or tumor cells in the period of mass death when exposed to inhibitory factor or vibrations autochthonous microorganisms of the mammal during the period of exposure to BL is favourable stimulating factor, providing intensive development and reproduction of autochthonous microorganisms in the logarithmic growth phase.

2. The method according to claim 1, characterized in that as an inhibitory factor used antibiotics, or sterilizing agents, or exposure to heat or ultraviolet or radiation exposure.

3. The method according to claim 1, characterized in that as a favorable stimulating factor, providing intensive development and reproduction of autochthonous microorganisms in the logarithmic growth phase, use bifidogenic, lactogenic growth factors, vitamins and trace elements.



 

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