Hepatoprotective herbal tea. RU patent 2500418.

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, particularly to a hepatoprotective herbal tea. The hepatoprotective herbal tea that contains black currant leaves, red raspberry leaves, great willow herb, meadowsweet herb in certain proportions.

EFFECT: herbal tea provides the effective hepatoprotective action.

2 tbl

The invention relates to medicine, in particular to the pharmacy, and represents the collection of medicinal vegetable raw materials with a hepatoprotective effect.

Known collection, which has and activities (RF Patent №2419447 C2, pub. 27.05.2011), inflorescence contains calendula; mint leaves; the roots of Scutellaria baicalensis; bergenia crassifolia rhizomes; leaves black bergenia crassifolia; shoots shrub and used for the treatment and prevention of alcoholic hepatitis.

There is a method of complex treatment of chronic viral hepatites b and C (RF Patent №2341275 C1, pub. 20.12.2008), including, President of the collection №1 of medicinal plants containing oak bark, grass clover, licorice roots, grass Polygonum aviculare, burdock root, leaves mother-and-stepmother, grass desertsrnika grass sushenitsy, herb Echinacea, and during the phase of remission, President of the collection №2, containing the rhizomes with roots of elecampane, calendula flowers, leaves of nettle, leaves of plantain, fruit trees, grass wormwood pine, sash beans, herb Echinacea.

A method of obtaining funds, has and choleretic action (RF Patent №2292897 C1, pub. 10.02.2007). The method consists in the extraction of the crushed material and separation of the extract, used as raw material collection of medicinal vegetative raw material : herb of Salsola Collina herb, celandine, tansy flowers and conduct consistent triple extraction of 70%ethanol and water purified at the ratio of raw material : extractant 1:1 by the method of stepwise countercurrent extraction.

Known hepatoprotectors of plant origin - legal (silymarin, silibinin), Kars, derived from milk Thistle fruit, which are the most similar pharmacological activity to the proposed collection of medicinal plants and are recommended as a reference product in studying hepatoprotective medicines [ .. Guide experimental (pre-clinical) study of new pharmacological substances., Moscow. 2005, .683-691].

The task of the invention - expanding Arsenal hepatoprotective of vegetable origin.

The problem is solved by collection of hepatoprotective action, including the leaves of black currant, raspberry leaves pine, grass fireweed herb and grass Filipendula ulmaria when equally weight ratio.

Use this collection as a means of hepatoprotective became possible due to the experimental determination of its new properties, namely medical and preventive action against toxic liver. Water infusion from the proposed collection has expressed activity, equal to a reference product - . The ability of this collection to prevent and mitigate the consequences of toxic liver injury found in carrying out experimental research.

Specific example of the

Dried, crushed and sifted through a sieve with a diameter of 0.5 mm raspberry leaves ordinary 10,0; leaves of black currant 10,0; grass fireweed herb 10,0; grass Filipendula ulmaria 10,0 mix, placed in a capacity of 1000 ml, add 400 ml of distilled water with a coefficient of water absorption (For EAP =3,0). Heated to boiling water bath for 15 minutes, cooled at room temperature for 45 minutes After removing the cooling filtered through a gauze filter.

At the first stage of research was conducted a comparative study of treatment-and-prophylactic actions developed by the collection and a reference preparation - Kars when CCl 4-induced toxic hepatitis. The research was made on 60 rats-males weighing 220-240 g, randomly distributed over 4 groups:

Group 1-intact rats (n=22);

2nd group-control group (n=22);

Group 3 test, the proposed fee (n=28);

4 group experienced a reference drug - Kars (n=28).

The animals were kept in standard vivarium conditions on a standard diet feeding. The first experimental group during the three weeks received water extraction from the proposed collection. During the first two weeks of the experiment experienced animals received an infusion in the conditions of free-seal, on average one animal had to 30 ml of infusion in the day. The second experimental group received Kars (Sopharma, Bulgaria) in the dose of 200 mg/kg intragastric in the form of mist, as a reference drug with activity. Animals of the control and the control group received water ad librum.

On the 15th day of the experiment in the experimental and control group of animals called toxic hepatitis intra-peritoneal introduction of CCl 4 a 25% solution in olive oil, one-time dose of 0.2 ml/kg (in terms of CCl 4 ). After which the animals continued to receive hepatoprotectors for a period of 7 days. Thus, in the experiment simulated a therapeutic and prophylactic use of hepatoprotectors.

Hepatoprotective effect of the investigational collection was estimated using sample. Thiopental were injected in a dose of 50 mg/kg and recorded the duration narcosis in terms of «lateral position». The sample allows to estimate the rate of metabolism of thiopental, implemented by the cytochrome P-450-dependent monooxygenase system of hepatocytes.

The results of the experiment were processed using a student's t-test and presented in table 1.

Table 1

The effect of aqueous extracts from the collection of medicinal plants and prototypes on the duration narcosis in animals (min)

No. n\n

Group of animals

The number of animals in the group

} the x±m

1.

12

59,8±6,27

2.

Control

12

KZT 168.1±9,68*

3.

Experience the inventive collection)

18

40,7 ħ 4.83*;**

4.

Experience (cars)

18

46,9±3,84**

Notes: * significantly different from (P<0.05);

** - significantly different from the control (P<0.05).

CCl 4-hepatitis is characterized by the development necrosis, protein and fatty degeneration of hepatocytes, localized mainly in the Central zone of hepatic segments, where the maximal activity dependent on cytochrome P-450 and production dominates damaging metabolites . From the data obtained in the experiment shows that duration narcosis in animals exposed to (control), duration narcosis was almost 3 times higher than that of . Therapeutic and prophylactic use of the studied fee is fully warned these CCl 4-induced changes. It should be noted that the comparison of hepatoprotective effect on this indicator (duration narcosis) did not reveal statistically significant differences from the same indicator comparison drug - Kars. This indicates less liver damage in the experimental group (the proposed acquisition) and the preserved functions cytochrome P-450-dependent monooxygenase system of hepatocytes.

As informative criteria for the evaluation of conservation of the liver tissue and effectiveness of the proposed collection were also used some biochemical and blood counts.

In experimental animals, not exposed to thiopental, on the 21st day from the start of the experiment, after , took the blood to determine cholesterol, bilirubin (total, direct, indirect), ALT, AST. The blood was centrifuged for 10 min at 3000 rpm in a centrifuge «Scanspeed mini» (USA). Selected plasma and evaluated the biochemical indices of the blood using a set of firm «Intermedica» (USA)according to manufacturer instructions on the biochemical analyzer firm «Biochem» (USA).

Table 2

The change of the basic biochemical indices of the blood serum of rats in applying the proposed collection on the model of an experimental hepatitis

The basic biochemical indicators

In intact animals

Control, CCl 4

Experience: CCl 4 + claimed collection

Experience: CCl 4 + Kars

ALT, ME

35,0±3,6

220,5±24,8 *

63,1±6,0 *;**

54,3±6,19 **

ACT, ME

136,8±14,7

1326,8±RUB 184.6 *

570,3±61,4 *;**

487,4±57,8 **

Cholesterol, mmol/l

1,41±0,05

2,68±0,10 *

1,89 ą 0.05 *;**

1,75±0,05 **

Bilirubin total mkmol/l

10,88±0,34

134,68±8,00 *

90,10±6,60 *;**

87,6±7,05 **

Bilirubin direct mkmol/l

3,18±0,17

64,05±2,05 *

38,10±3,08 *;**

37,21±3,11 **

Unconjugated bilirubin mkmol/l

7,70 approximately 0.5

78,63±7,07 *

52,00±of 3.93 *;**

50,39±3,92 **

Note: * - significantly differ from (P<0.05);

**- significant difference with the control (P<0.05).

As can be seen from the results presented in table 2 in the control group CCl 4 hepatitis there was a significant increase in the level of liver enzymes, cholesterol, total, direct, and indirect bilirubin, caused by exposure to . In the group receiving the proposed fee on the background of hepatitis, these shifts were significantly less pronounced. It should be noted that the data obtained in the group of «CCl 4 + claimed collection» are only slightly inferior to the results obtained in the group of animals «CCl 4 + cars».

Thus, the data obtained in the experiment was evidence of the hepatoprotective properties of the collection, consisting of raspberry leaf pine, leaves of black currant, grass fireweed herb and grass meadowsweet.

Collection of hepatoprotective action, characterized in that it contains the leaves of black currant, raspberry leaves pine, grass fireweed herb and grass Filipendula ulmaria when equally weight ratio.