Method for prevention of respiratory disease and improving fertility in young deers
 Adsorbing product / 2382655Described is adsorbing product, such as sanitary towel, everyday towel, tampon, diaper, diaper in form of underpants, protective means for incontinence for adult people, provided with at least one capsule (8), containing bacterial composition (11) in lipid phase (12), which preferably contains, at least, one strain of bacteria producing lactic acid. At least one part of capsule has minimal transversal size (a), equal to at least 2 mm. Capsule (8) preferably includes core (9), which contains bacterial composition (11) in lipid phase (12), and coating (10), preventing environment impact on core during transportation and storage. |
 Method for treatment of gastrointestinal diseases in calves / 2381806Method includes bottle-feeding of EM-preparation "Baykal EM-1" to calves in mixture with infusion of herbal tea in ratio of 1:1. At the same time herbal tea contains the following components (per 100 ml of water): 0.5 g of touch-and-heal herb, 0.5 g of meadowsweet herb, 0.35 g of thousand-leaf herb, 0.35 g of stinging nettle, 0.2 g of Bergenia crassifolia rhizome, 0.2 of roots and rhizome of cowberry and 0.15 g of Scotch pine needles. Mixture is bottle-fed according to the following scheme: 1 day - twice in doses of 30 ml and 50 ml; 2 day - thrice in dose of 100 ml; further - five times a day in dose of 100 ml until clinical recovery. |
 Adsorbent product / 2380118There is described adsorbent product, such as a hygienic towel, a daily liner, a tampon, a diaper, a diaper panties, a protective aid for incontinence in adults, containing a bacterial composition (8) in lipid phase (9), and the specified bacterial composition contains at least one strain of bacteria producing lactic acid. The lipid phase (9) containing the specified bacterial composition (8), is applied on a carrier element (10) or therein. The carrier element, at least before the product is applied, is impenetrable to the lipid phase. The carrier element is located on the side of the adsorbent structure (4) turned to the user so that to prevent considerable transmission of the lipid phase in the adsorbent structure. |
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Method of preparing probiotic drug / 2380107 Method involves preparation of a stock culture through combined growth of lactic acid bacteria Enterococcus faecium B 3491, Lactobacillus plantarum B 3242, Bifidobacterium bifidum AC 1247 under submerged cultivation conditions, taken in ratio of 1:1:1 until achieving bio-titre of each bacterium of not less than 5·108 CFU/ml. Growth is carried out in a liquid medium which is a mixture of cow milk and water in ratio of 1:1. A mixture of extraction cakes-aromatic herbs is prepared from cumin seeds, coriander seeds and parsley herbs after CO2 extraction with the following ratio of components, wt %: extraction cake of cumin seeds 20-40, extraction cake of coriander seeds 30-50, extraction cake of parsley 30. The obtained mixture of extraction cakes is inoculated with the stock culture in ratio of 1:1 and incubated at 35-40°C for 4-6 days to obtain not less than 5·109 CFU/g in a semihumid preparation. The obtained semihumid preparation is mixed with the obtained mixture of extraction cakes until attaining moisture content of 8-12% in the ready preparation and a bio-titre of each microorganism of not less than 5·108 CFU/g. |
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Microbiological method for preparation of 7α-hydroxyandrostenes / 2377309 Method of selective preparation of 7α-hydroxyandrostens with the help of floccus, includes transformation of Δ5 -androstenes with the help of mycelium Curvularia lunata VKPM F-981 separated and washed from medium. At the same time substrate is used in the form of microchips, or in the complex with cyclodextrins. |
 Method of treating gastrointestinal diseases in calves / 2377005Method includes internal application of medication "EM-kurunga" in form of kurunga metheglin in mixture with infusion of phytopreparation, which includes in terms of per 300 ml of water 1.0 g of St. John's wort herb, 1.0 g of honeysweet herb, 0.7 g of sanguinary herb, 0.7 g of stinging nettle herb, 0.4 g of leather bergenia herb rhizomes, 0.4 g of roots and rhizomes of comarum palustre and 0.3 g of common pine needles, 300 ml of water, in ratio 1:1. Mixture is kept to mature at temperature 18-20°C during 24 hours and applied internally in accordance with the following scheme: 1 day - twice in doses 30 ml and 50 ml; 2 day - three times in dose 100 ml; in following days - five times in dose 100 ml to clinical recovery. |
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Method for making dry synbiotic preparation / 2375069 Mother culture is prepared by consistent cultivation of lactic microorganisms Lactobacillus acidophilus B 3235, Lactobacillus delbrueckii subsp.bulgaricus B 5788, Bifidobacterium longum AC 1249, Bifidobacterium adolescentis AC 1244, taken in equal proportion on the nutrient medium consisting of the following components, wt %: cow's milk 50, glucose 5-15, lactose 5-15, agar 3-4, water - the rest. Solid state fermentation of the mother culture is carried out on sterilised amaranth flour in the ratio 1:1 at 35-40°C during 4-6 days to produce a semihumid preparation of 2·109 CFU/g and more. The latter is mixed with the sterilised amaranth flour to ensure humidity of the finished preparation 8-12% and of the biotitre 2-108 CFU/g and more. |
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Therapy of otoacariasis in fur-bearing animals / 2375068 Invention concerns veterinary science and can be used in fur breeding in case of development of otoacariasis. The therapy involves single subcutaneous introduction of preparation Bovinet in a dose 0.2 mg/kg of animal's weight. |
 Medicine for treatment of gastrointestinal tract diseases in chickens / 2371190Medicine contains symbiotic system of strains of lactic acid bacteria Lactobacillus acidophilus D-75 and D-76 of medicine "Vitaflor", enrofloxacin and twin-80 with following ingredients ratio (% wt) symbiotic microorganisms of "Vitaflor" medicine - 10-20; enrofloxacin - 75-85; twin-80 - 1-2. |
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Method of treatment of purulo-necrotic wounds in necrobacillosis in reindeers / 2368382 Method consists in using a substance consisting of 9 parts of biologically active hydrolyzate of velvet antlers and 1 part of bacterium Bacillus subtilis "ТНП-3-ДЕП" strain suspension, containing 10 billions of microbial cells per 1 ml. |
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Method for preventing chlamydial gastroenteritis in calves / 2245156 It is suggested to apply sulfatetrine M and probiotic ocarine for calves by the following dosage-temporal scheme: sulfatetrine M at the dosage of 0.2-0.4 g/kg body weight starting since the second colostral feeding, and then at a 12-h-long interval for 7 d; ocarine - at the dosage of 8x106 microbial cells once daily for nocturnal period for 10 d. The suggested combination enables to considerably decrease side effects, provides immunocorrection of the main affected immunity links, for example, prophylactic efficiency of the method corresponds to above 90% at 100% safety of calves. |
 Method for prophylaxis of chlamydium bronchopneumonia in calves / 2246309Method involves applying furacyclin M as a tetracycline preparation and probiotic -ocarin additionally for 10 days that are given to calves from 12 old days age by the following schedule: furacyclin M in the dose 0.4-0.5 g/kg of body mass, 2 times per 24 h, and ocarin in the dose 8 x 106 bacterial cells, 1 time per 24 h before night. Invention promotes to enhancing functional activity of blood neutrophiles, elevating content of T- and B-lymphocytes and activating their function that, in turn, promotes to enhancing the natural resistance of calves body. Invention can be used for prophylaxis against chlamydia bronchopneumonia in calves. |
 Preparation for preventing transport stress in cattle youngsters / 2251261The suggested biopreparation named "Lactobifadol" should be applied 1 mo before the impact of the mentioned stress factor at the dosage of 100-200 mg/kg body weight, daily. Application of "Lactobifadol" enables to normalize physiological body state and decrease the losses of meat production. |
 Healthful and dietary product, method for production and uses thereof / 2252770Claimed product contains dry biomass of living bacteria Lactobacillus rhamnosus odi 83 and Lactococcus lastic ssp lactis odi 83, and filler. Filler represents dry fermented milk product obtained by fermentation of milk and honey mixture with strains Lactobacillus rhamnosus odi 83, Lactococcus lastic ssp lactis odi 83, Saccharomyces cerevisae odi 83, and contains biomass of inactivated microorganisms mentioned above. To obtain healthful and dietary product pasteurized milk is cooled to 20-25°C, honey is added, ferment containing strains Lactobacillus rhamnosus odi 83, Lactococcus lastic ssp lactis odi 83, and Saccharomyces cerevisae odi 83 is introduced, fermentation is carried out; then obtained mixture is blended and sterilized; cooled again to 20-25°C, two strains: Lactobacillus rhamnosus odi 83 and Lactococcus lastic ssp lactis odi 83 is introduced, and fermentation is carried out again up to acidity of 85±15°T. After process finishing mixture is thoroughly agitated and dried at 35-40°C up to residue humidity. For treatment of infective and non-infective disorders claimed product in therapeutically effective amount is administered to patient in need of such treatment. |
 Cosmetic composition for lose weight comprising substance inducing il-6 / 2258500Invention relates to cosmetic composition used for loss weight comprising at least one compound that induces producing IL-6 by adipocytes in form of mixture with NPY antagonist and/or α2 antagonist and with an excipient for the cosmetic variant. Invention provides effectiveness of fatty acids efflux from adipocytes and inhibitory effect on fatty acids incorporation into cells that results to the loss weight effect. The composition shows good stability in storage being without reducing its activity. The composition has no adverse effects. |
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Method for preventing bronchopneumonia in piglets / 2258523 During the 1st d after forming raising groups one should once inject intramuscularly a solution of "Complex A" preparation at the dosage of 0.30-0.32 mg/kg body weight for 45-60-d-aged piglets and simultaneously with fodder one should introduce "Lactobifadol" preparation at the quantity of 0.50-0.52 g/kg body weight during the first 1-5 d. The present innovation enables to activate immune system, increase total protein and hemoglobin in blood that in its turn, prevents bronchopneumonia and remove stress in piglets after forming special raising groups. |
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Method for production of probiotic preparation / 2266126 Lactobacillus acidophilus Scav.B-4625, Rhuminococcus albus Kr, Bacillus subtilis B-8130 from Bacillus subtilis microorganisms are separately cultivated under conditions of deep cultivation, wherein Lactobacillus acidophilus Scav.B-4625 and Rhuminococcus albus Kr are cultivated in liquid broth, containing rumen liquid, sunflower oilcake, meat-and-peptone broth, sodium chloride, magnesium sulfate, disubstituted potassium phosphate, ammonium sulfate, calcium carbonate and water in specific component ratio for 4-6 days to obtain titer for each microorganism of 1.108 - 3.108. Strain Bacillus subtilis B-8130 is cultivated in liquid broth, containing yeast, molasses, sunflower oilcake, gluten, magnesium sulfate, disubstituted potassium phosphate, calcium carbonate and water in specific component ratio for 4-6 days at 37-45°C, adjusting pH up to 7.2-7.4 to obtain titer for Bacillus subtilis B-8130 of 1.108 - 2.108. Then microorganism cultural liquids are blended in the next ratio (mass %): Lactobacillus acidophilus Scav.B-4625 and Rhuminococcus albus Kr cultural liquids 45-55; Bacillus subtilis B-8130 cultural liquid 45-55. |
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Probiotic composition for animals and poultry / 2266747 The present innovation deals with biopreparations for agriculture indicated for prophylaxis and treatment of poultry diseases. The probiotic composition suggested contains the strain Rhuminococcus albus Kr, the strain Bacillus subtilis VKPM V-8130 and Lactobacillus acidophilus Scav. VKPM V-4625 at equal quantities. The innovation combines antimicrobial body protection along stimulating impact upon it at simultaneous increase of fodder conversion at high content of fiber, broadened assortment of curative-prophylactic preparations of natural origin. |
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Method for treating and preventing piglet nematodosis / 2274462 Method involves administering Aversect-2 with Cordeon probiotic added at a dose of 50 mg/kg of live weight during 5 days. |
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Method for preparing dry probiotic preparation "batsell" / 2280464 Invention relates to technology for preparing bacterial preparations used in prophylaxis diseases in animals. Method involves the separate culturing under conditions of submerged culturing of microorganisms Bacillus subtilis VKPM B-8130, Lactobacillus acidophilus Scav. VKPM B-4625 and Rhuminococcus albus Kr. The grown mother cultures of B. subtilis VKPM B-8130, L. acidophilus Scav. VKPM B-4625 and R. albus Kr in the ratio = 1:1 and autoclaved sunflower grist is inoculated with this mixture in the following ratio of components, wt.-%: autoclaved sunflower grist, 45-55; mother culture of Lactobacillus acidophilus Scav. VKPM B-4625, Rhuminococcus albus Kr, 20-30, and mother culture of Bacillus subtilis VKPM B-8130, 20-30. The prepared mixture is incubated at temperature 30-45°C for 4-8 days to obtain 1 x 108 cells/g of preparation in semi-wet preparation, and prepared preparation is mixed with sunflower grist in the ratio = (1:10)-(1:12) to obtain the preparation with residual moisture 8-10%. Invention provides enhancing effectiveness in preparing the dry probiotic preparation, reducing material consumptions in its production, simplifying technology for its preparing and using inexpensive raw in preparing the preparation based on exclusion the drying step from the technological schedule and preparing the preparation with higher titer value. |
FIELD: medicine, veterinary science.
SUBSTANCE: an antibacterial agent, probiotic "Sachabactisubtyl" from strains "Bacillus subtilis "ТНП-3-ДЕП"" and "Bacillus subtilis "ТНП-5-ДЕП"", adsorbed on zeolite of Honguru deposit.
EFFECT: method allows improving immunobiological eactance of an organism, fertility and preservation of young deers.
The invention relates to veterinary medicine, in particular to the prevention of respiratory diseases and improve productivity of deer.
Known methods of prevention of respiratory diseases by administration of antibiotics (Berdiev B.N., Eglit GU, Sleptsov V.P. "the Preparation and conduct of calving deer, saving the resulting offspring" Novosibirsk, 1993. - 20 C.).
However, the use of antibiotics does not give the desired result. The uncontrolled use of antibiotics contributes to the development of drug-resistant substances highly virulent strains of microorganisms.
Known way to prevent diarrheal disease of newborn calves and piglets by using suspensions of strains of bacteria Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT" (patent of the Russian Federation "the Way to prevent diarrheal disease of newborn calves and piglets No. 2218924, Or 1/100, publ. 20.12.2003,, bull. No. 35). However, there is no information about the use of probiotic "Sakhabaktisubtill", consisting of strains of bacteria Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPOT", as microbial drug for the prevention of respiratory disease and increase the productivity of reindeer. The basis of the invention is to develop a method of prophylaxis of respiratory diseases of the respiratory system by microbial drug adsorbe is consistent on the zeolite.
The technical result of the invention is the prevention of respiratory diseases and increase the productivity of reindeer by inclusion in the diet of young deer microbial drug adsorbed on the zeolite changuinola field. Using strains of bacteria Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT", which are not only antagonistic activity against many pathogenic and conditionally pathogenic microorganisms, but also by complex enzymatic activity (proteolytic, gelatinase, amylase, xylanase), as well as the ability to normalize intestinal microbiocenosis, to stimulate immunobiological reactivity of the organism, to increase the productivity of reindeer.
The technical result of the invention is achieved as follows:
Probiotic "Sakhabaktisubtill", consisting of strains of bacteria Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT", adsorb on the zeolite changuinola field. One dose contains 5 grams of zeolite and 100 billion CFU of strains of bacteria Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT".
The agent shall feed once a month.
Microbial drug adsorbed on the zeolite, stimulates physiological and biochemical processes, immunological reactivity, suppresses the development of pathogenicities, thereby renders prophylactic action of respiratory diseases, increases average daily weight gain, increases the safety of the deer.
Probiotic "Sakhabaktisubtill" made from strains of bacteria Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT", deposited in the collection of microorganisms of the all-Russian state Institute of control, standardization and certification of veterinary preparations (VGNKI). Certificate of Deposit issued February 6, 2001, No. 180/15 and 181/15). The bacterial strains Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT possess antagonistic activity against pathogenic microorganisms and stimulate immunobiological reactivity of the organism, and are characterized by their ability to produce enzymes that the body of the deer does not synthesize.
Strains can be obtained in the laboratory on the development of microbial agents wildebeest Yakut scientific research Institute of agriculture of Russian agricultural Academy and VGNKI.
Fabrication, inspection and testing of the drug "Sakhabaktisubtill" is carried out according to the specifications of the Drug Sakhabaktisubtill for the prevention and treatment of diarrhea of newborn calves No. 9383-003-00670203-01 of March 12, 2001
The bacterial strains Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT" grown in mesopatamia agar pH 7.0 to 7.2 for 5 days at 37°C, then suspension equally appropriate is osenia a 2%solution of sodium chloride at a concentration of 5 billion microbial cells in 1 ml Zeolite changuinola field of fractions of the flour is washed, dried at 150°C, for the preparation of 1 dose 5 g of zeolite is made of 20 ml of suspension contains 100 billion CFU of strains of bacteria Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT", for 60 minutes at room temperature.
To prevent disease and improve productivity of selected 30 goals weakened and exhausted young animals 12 months of age with symptoms of respiratory diseases.
The first group of young (15 goals) designate 1 dose monthly for 2 months. Carry out clinical examination, blood for biochemical analyses and examinations of stool samples taken before and after the experiments, take into account productivity. The control group (20 animals) is not receiving the drug.
In the blood of young animals receiving the drug, increased the total protein content (experience - 8,12±0.008 g/%, control - 0,121±0,002 g/%) and its fractions. Especially increasing the number of gamma-globulin (experience - 0,249±0.02 g/%, control - 0,121±0,002 g/%). Increases bactericidal activity of blood serum (experience - 43,38±4.9%, control - 29,68±1,98%).
It was found that after use of the drug increases the number of the main representatives of the normal flora of the intestine:
bifidobacteria (1966,6±Tyskie/g), and 50% of the number of examined animals established the presence of lactobacilli in the middle 0,245±0,15 Tyskie/g, whereas the mole is dnace control group of lactic acid bacteria are not detected.
The number lactosaminated Escherichia in the control group above 8 times than experienced. While in young control group allocated pathogenic staphylococci. Spore-forming aerobic bacteria present in the intestine in both groups, the experimental group the number dominates (4700,0±427,14 Tyskie/g).
In the experimental group marked improvement in General condition, appearance of appetite. After 12-15 days after giving the drug is installed recovery and transfer to the main herd. On the 40th day after the appointment of the drug significantly increases the average live weight (experience - 60,21±1.2 kg, control - 51,29±2.4 kg).
Thus, the application of probiotic "Sakhabaktisubtill"adsorbed on the zeolite changuinola field increases immunobiological reactivity of the organism, normalizes intestinal microbiocenosis, thereby contribute to the prevention and treatment of respiratory diseases, increase productivity and security.
The way to prevent respiratory diseases and increase the productivity of reindeer by inclusion in the diet of antibiotics, characterized in that as an antibacterial agent used probiotic "Sakhabaktisubtill from strains of Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT"adsorbed on the zeolite changuinola field.