Method for predicting clinical mammary gland carcinoma course |
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IPC classes for russian patent Method for predicting clinical mammary gland carcinoma course (RU 2300111):
                  
Method for detecting extracranial metastases in metastatic brain injury patients / 2300110
Method involves determining cortisol hormone concentration in cerebral metastasis-ill patient blood prior to treatment beginning. Its concentration being found within the limits of 54.0 to 130.0 nmole/l, metastatic lesion foci are predicted to be available in other internal organs and tissues.
Method for predicting cerebral metastasis lesion foci in metastatic brain injury patients / 2300109
Method involves determining thyroxin hormone concentration in cerebral metastasis-ill patient blood prior to treatment beginning. Its concentration being found within the limits of 68.0-115.0 nmole/l, 1 to 3 metastatic lesion foci are predicted to be available. The concentration being from 38.0 to 50.0 nmole/l, more than 4 p metastatic lesion foci are predicted to be available.
Method for predicting extracranial metastases development in patients having metastases in brain / 2297005
Method involves determining adrenal glands cortex hormone cortisol content in blood. At its level below the bottom border of the norm making from 260.0 up to 450.0 nmole/l, within the limits of cortisol concentration fluctuations from 70.0 to 240.0 nmole/l, extracranial metastases development are predicted to occur in 1-3.5 months after primary examination.
Method for predicting physical growth and development of small children / 2291444
Method involves determining triiodothyronine content in peripheral blood serum of children of the second half-year of life having perinatal central nervous system injury manifestations and no signs of hypothyroidism. The value being equal to 0.69±1.34 ng/ml, the children are predicted to have small height to the third year of life.
 Method for carrying out monitoring of diabetes mellitus patients state and neurological and vascular complications development / 2291437
Method involves determining immune reactivity of blood serum with respect to insulin, to anti-insulin antibodies or their antigen-binding fragments, to anti-anti-insulin antibodies binding the antibodies to insulin and antigens to growth factor or their antigen-binding fragments and ANCA antigen. Blood serum immunoreactivity increase with respect to parameters under measurement relative to norm is used for determining diabetes mellitus neurological and vascular complications development.
 Method for predicting the phases of stress states / 2288475
In patient's blood one should detect the concentration of cortisol and fertile factors α2-microglobulin (AMGF), trophoblastic β1-1-glycoproteide (TBG) and α1-placental microglobulin (PAMG-1). The phases of stress states should be diagnosed by the coefficient calculated due to dividing the sum of AMGF and TBG concentrations by the sum of PAMG-1 and cortisol concentrations, moreover, the concentration should be expressed in % against average values in healthy persons. Coefficient's value being equal to 1.0-11 corresponds to the norm; at its value being 1.2-1.5 one should diagnose the phase of anxiety; at 0.7-0.9 - the phase of resistance; at 0.2-0.6 - the phase of exhaustion. The innovation provides the chance to program the flow of pathological process more accurately.
Method for predicting the development of gastrointestinal tract paresis in patients with vertebral traumas / 2286089
In patients with vertebral traumas for about 2-3 h after lesion one should simultaneously determine the level of hormone cortisol, malonic dialdehyde (MDA) in blood serum, and, also, the value of total bioelectric activity (TBEA) of gastrointestinal tract (GIT) and at cortisol values being 981.7 nM/l and higher, and MDA values - 8.35 mM/l and higher and values of TBEA being 387.75 mcV and lower it is possible to predict the development of GIT paresis.
Method for predicting the development of stress reactions in early neonatal period in neonatals born in females with urogenital infection / 2284526
In serum of peripheral blood in pregnant women in trimester III of pregnancy period it is necessary to determine serotonin concentration. The flow of stress reactions in early neonatal period in neonatals should be predicted at serotonin level being above 0.1 mcg/ml. Application of the present method provides the opportunity for earlier prediction of the development of stress reactions in neonatals in early neonatal period.
Method of prognosing generalization of process in children and adolescents with osteal sarcoma / 2282195
Method provides for early prognosis of generalization disease due to most wide biological action of thyroidal hormones regulating principal stages of metabolic processes in body and influencing proliferation velocity and functions of nervous and immune systems, whose status, in turn, greatly determines state of antitumor résistance. Method is characterized by performing complex antitumor treatment and biochemical investigation and in patients undergone complex treatment with complete clinical and laboratory remission level of free fraction of thyroidal hormone triiodothironine is determined in blood. When this level lies within a range of 4.0 to 5.2 pmol/ml, preservation of remission during 6 to 24 month is forecasted and, at the level 5.8 to 7.4 pmol/ml, generalization of malignant process within period of 1 to 3 months is expected.
Method for diagnosing obliterated form of congenital adrenal gland cortex hyperplasia / 2279090
Method involves carrying out test with adrenocorticotropic. Blood levels of 11-desoxycortisol (S), cortisol (F), cortisone (E) and corticosterone (B) are determined by means of high performance liquid chromatography. Ratios of cortisol-to-cortisone levels (F/E) and to 11-desoxycortisol level (F/S) are calculated. Corticosterone level being lower than 17 ng/l and 11-desoxycortisol level being greater than 7.0 ng/l with F/E<5 and F/S<16, obliterated form of congenital adrenal gland cortex hyperplasia is to be diagnosed.
Method of determining lymphadenotropic effect of recombinant human interferon-alpha2 preparation / 2300105
Invention relates to laboratory diagnostic methods and consists in that a single nontoxic dose of glycerol-containing solution of recombinant human interferon-α2 is introduced into oral cavity of experimental inbred mouse BALB/c, which is killed 30-60 min later simultaneously with an intact mouse, after which cryostat and paraffin histological sections of lymph node tissue of experiment and intact mice are prepared and, in the comparable histological sections of lymph nodes of the two mice, interferon-α is determined using indirect immuno-fluorescent assay method revealing tissue basophiles tinted according to Romanovsky-Gimsa's procedure. When comparing intensity of specific illumination and number of tissue basophiles, lymphadenotropic effect of recombinant human interferon-α2 preparation is determined.
Method for predicting brain metastatic injuries generalization / 2300104
Method involves studying blood plasma chemoluminescence intensity and nitrogen oxide derivatives content. Total percent of changes of said indices relative to reference value is calculated. The change being insignificant like reduction from -21.7% to -148%, single cerebral metastasis is diagnosed. The value growing from 200% to 1000%, multiple metastatic brain injury independent of primary tumor lesion focus localization is diagnosed.
Method for complex treatment of resectable forms of endometrial cancer / 2297798
One should carry out surgical interference , distance gamma-therapy and hormonotherapy with aromatase inhibitors. Moreover, after surgical interference it is important to determine the level of aromatase in remote tumor, at its value being 7fM androstenedione/mg protein/h and more, despite the receptor status one should prescribe hormonotherapy with aromatase inhibitor - anastrosol - at the dosage of 1 mg/d once for 6 mo running on finishing distance gamma-therapy. Such a complex therapy due to taking into account the threshold value of aromatase level in tumor enables to decrease the frequency of local relapses considerably during the first 2 yr of life after therapy conducted, in case of minimal quantity of therapeutic complications.
Method for selecting pregnant women during i-ii trimesters for carrying out the correcting therapy of thyroid dysfunction / 2297794
Beforehand it is necessary to detect the level of free thyroxine and thyrotropic hormone in blood serum to conduct ultrasound thyroid testing. Moreover, if the level of free thyroxine corresponds to or is below 12.1 pM/l, - 15th percentile of available normative values, and the level of thyrotropic hormone corresponds to about 2-4 mIU/l, in case of increased thyroid volume by 30%, not more against the norm and/or diffuse structural thyroid alterations it is important to prescribe preventive therapy for pregnant women with levothyroxine preparations at the dosage of 25-75 mcg/d. The innovation enables to prevent the complications of pregnancy and improve perinatal result.
Method for evaluating functional state of immune system of palatine tonsils in patients with paratonsillitis / 2297631
In the tissue of palatine tonsils in patients with paratonsillitis it is necessary to detect Epstein-Barr virus (EBV) for determining the content of salivary immunoglobulin G. At detecting EBV in bioptate and IgG content being below 20 mcg/ml one should determine the functional failure of immune system of palatine tonsils.
Method for predicting tuberculosis etiology of chorioretinitis / 2297186
One should apply tuberculin samples for testing the picture of an eyeground through fixed time intervals. Ultrasound dopplerography should be used for detecting in central retinal artery (CRA) and posterior short ciliary arteries (PSCA) the values of circulation the alteration of which should be registered twice - before tuberculin samples and in 48 h. Moreover, one should register the alterations of maximal systolic velocity of circulation - Vs, minimal diastolic velocity of circulation - Vd and index of peripheral resistance - Ri; one should investigate initial state of systemic humoral immunity. Then, at the decrease of Vs threshold value by 25% and Ri increase - by 26% in CRA, and, also, Vs and Vd decrease by 35% and Ri increase by 40% in PSCA, moreover, at decreased quantity of CD22 lymphocytes by 26%, Immunoglobulins A and G by 13%, considerable increase of circulating immune complexes (CIC) ranged 64-87%, increased content of complement C3 and C4 components by 57% and 179%, correspondingly, the decrease of total hemolytic complement activity by 30%, increased content of immunoglobulin M ranged 9-27% it is possible to diagnose tuberculosis etiology of chorioretinitis. The innovation enables to state upon tuberculosis etiology of chorioretinitis at minimal manifestation of focal reaction in ocular tissues, it excludes pilot therapy and enables to shorten the terms of etiological diagnostics of tuberculosis chorioretinitis considerably.
Method for detecting the predisposition to addictive disorders in the form of alcoholic addiction and/or co-addiction / 2296994
The present innovation deals with detecting genetic markers in human biological liquid. So, in a patient's saliva due PCR technique it is necessary to determine the frequency of alleles and genotypes of DRD2 and DAT genes and at detecting the increase of genotype A1/A2 of DRD2 gene and allele 440 of 440-440 genotypes and 440-480 of DAT gene in males and females against the standard one should predict the predisposition to addictive disorders in the form of alcoholism and co-addiction. The, additionally, one should carry out psychological testing and according to correlation of increased mentioned genetic markers against detected psychopathological peculiarities it is important to establish the predisposition to either alcoholism or co-addiction.
Method for predicting inconsiderable abnormalities of fetal cardiac development / 2296329
It is necessary to inspect a pregnant woman, moreover, in her peripheral blood one should detect the concentration of metalloproteinase-3 (MP-3), C-reactive protein, antibodies to cardiolipin of IgG type, creatine phosphokinase (CPK) and calculate discriminant function (Y) according to the following formula: Y=3.3088-0.14996×A-1.55513×B-0.08019×C-0.0087×D, where A - the value of MP-3 (ng/mL); B - the value of C-reactive protein (mg/dL); C - the value of antibody to cardiolipin of IgG type (GPL U/ml); D - the value of CPK (U/I) and at Y<0 one should diagnose inconsiderable abnormalities of fetal cardiac development.
 Method for detecting the predisposition to oncological diseases and diagnostic kit for its implementation / 2296328
The declared method and multi-purpose diagnostic kit (DK) are indicated for carrying out medico-genetic analysis of simultaneously several mutations (polymorphisms) of different genes, those associated with some frequent multifactorial diseases, among them. Due to applying standard component of the mixture and standard oligoprimers, optimizing the conditions for performing polymerase chain reaction (PCR) and analyzing polymorphism of restriction fragments length (PRFL) the rate of such an analysis has been shortened up to 2-3 d. The innovation simplifies and cheapens the analysis in question and makes it useful for carrying out molecular investigations both in specialized centers and in multi-purpose laboratories.
Method for earlier predicting the syndrome of delayed fetal development / 2295132
It is necessary to investigate peripheral venous blood of a pregnant woman, moreover, during the period of 6-12-wk-long gestation one should determine the value of vasculoendothelial growth factor (VEGF) content and at its values being equal or below 20 pg/ml it is possible to predict the onset of the syndrome of delayed fetal development.
Method for evaluating the severity of pathological process flow at chronic diffuse hepatic lesions / 2244305
One should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
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FIELD: medicine. SUBSTANCE: method involves determining tumor cells percent and estimating receptor color in semi-quantitative way. Positive cells proportion is recorded and reaction intensity is determined from formula H=3xA+2xB+1xC, where A is the intensively stained nuclei percent; B is the moderately stained nuclei percent; C is the mildly stained nuclei percent. Receptor distribution is determined in addition in tumor units of mammary gland carcinoma. Sites of positive and negative expression and various expression intensity sites being found, the expression is considered to be heterogeneous. Uniform receptor distribution being observed in the tumor irrespective of staining intensity degree, the expression is considered to be homogeneous. Homogeneous steroid hormone receptor expression being observed in tumor, favorable clinical disease treatment course is to be predicted. When heterogeneous, the prognosis is unfavorable. EFFECT: high accuracy of prognosis. 2 cl, 10 dwg
The invention relates to medicine, specifically to Oncology, and relates to methods for predicting the course of cancer of the breast. There is a method of predicting the course of disease in breast cancer by immunohistochemical analysis of steroid hormone receptors, which consists in determining the percentage of tumor cells expressing the receptor, and semiquantitative assessment of the degree of expression of the receptors (figure 1). They expect the rate expression according to the formula: N=3×+2×+1×, where A - percentage of intensely stained nuclei; In the percentage of moderately stained nuclei; With the percentage of weakly positive nuclei. When N=20 for the estrogen receptors of the tumor is estrogen receptor-positive. For progesterone receptors, this figure should be 5 or more. The research is conducted on cryostatic or paraffin sections after fixation buffered formalin [1]. The disadvantage of this method is that the metric expression of steroid hormone receptors is not taken into account the nature of their distribution in the structures of malignant tumors. Object of the present invention is to improve the prognostic value of expression of receptors in assessing the likelihood of lymphogenous metastasis and multicentric the growth of breast cancer. The problem is solved by determining the percentage of tumor cells and semi-quantitative evaluation of color receptors, accounting for the proportion of positive cells and determining the intensity of the reaction according to the formula: N=3×+2×+1×, where A - percentage of intensely stained nuclei; In the percentage of moderately stained nuclei; With the percentage of weakly positive nuclei. And additionally, if you increase the X100, determine the distribution of receptors in tumor items breast cancer and the presence of areas with positive and negative expression, as well as areas with different degree of expression of the expression is considered heterogeneous, in the presence of a tumor uniform distribution of receptors, regardless of staining intensity expression is considered homogeneous and homogeneous expression of receptors for estrogen in tumors predicts favorable course of the disease, and in the heterogeneous - negative. The heterogeneous nature of the distribution of the degree of expression in the different structures of the breast cancer is illustrated in Fig.2-7. Homogeneous expression demonstrated on Fig-10. The essence of the proposed method is illustrated by the following examples. Example 1. Patient K. the study according to the proposed method by the formula H=3×+2×+1#x000D7; From where A - percentage of intensely stained nuclei; In the percentage of moderately stained nuclei; With the percentage of weakly positive nuclei. N=3×53+2×41+1×3=244. When zooming in ×100 in the preparations of the tumor revealed areas with mild, moderate and severe expression of estrogen receptors. This distribution is estimated as heterogeneous. In the study of regional lymph nodes was found that in 4 of the 9 had metastases. Example 2. Patient Century. the study according to the proposed method by the formula H=3×+2×+1×, where A - percentage of intensely stained nuclei; In the percentage of moderately stained nuclei; With the percentage of weakly positive nuclei. N=3×100+2×0+1×0=300. When zooming in ×100 in the preparations of the tumor, diffuse, revealed a pronounced expression of estrogen receptors. This distribution is estimated as homogeneous. In the study of regional lymph nodes was found that in none of 11 lymph node metastases were not detected. The proposed method was examined 72 patients with breast cancer. The results revealed that: homogeneous distribution of expression of the estrogen hormones found in 37 (51%) cases, heterogeneous in 18 (25%) cases, estrogen-negative cases are defined in 17 (24%) patients. The results clicks is developed using a one-way ANOVA, the criterion of "consent" X2comparisons of frequencies of occurrence of the characteristic, expressed in percent. We used the software package "Statistica 6.0 for Windows. Found significant differences in the manifestations of tumor progression in the cases of homogeneous and heterogeneous distribution of expression of receptors for estrogen in the structures of breast cancer: 1. The sizes of the lesions were greater in the heterogeneous expression of estrogen receptors in tumors of breast cancer than with homogeneous expression (respectively: 2,46±1,2 and 1,8±0,7; F=5,98; p=0.017). 2. Metastatic lesion of the axillary lymph nodes with heterogeneous expression was observed more frequently than in homogeneous (respectively: 72,2% and 30.5%; p=0.005). 3. Allotment expression of estrogen receptors associated with the stage of breast cancer. In cases with homogeneous expression of estrogen receptors stage I breast cancer were detected in at 45.9%, while in the heterogeneous expression of only 11.1% of cases (p=0,0067). The second stage of breast cancer met with homogeneous expression of estrogen receptors in 51,3%, while in the heterogeneous expression in 83.3% (p=0.013). 4. Unicentric tumor development and multicenter growth of breast cancer by identifying microfocused tumors were associated with the nature of the expression of receptors for estrogen. In cases unitentional tumor growth homogeneous RA is the distribution of the receptors was found in 80%, heterogeneous - 20%. When the multicenter growth of breast cancer, on the contrary, homogeneous distribution of receptors for estrogen rarer (33,3%) in comparison with heterogeneous (66,6%). A similar relationship between the type of tumor growth and distribution pattern of expression of estrogen receptors reliable (x2=8,4; p=0.004). Literature 1. Petrov S.V. Guide immunohistochemical diagnosis of human tumors // Svitlov, Nutricline. Kazan, The Title. - 2004. - S-117. 1. A method for predicting the course of the disease breast cancer by immunohistochemical analysis of steroid hormone receptors by determining the percentage of tumor cells and semi-quantitative evaluation of color receptors, accounting for the proportion of positive cells and determining the intensity of the reaction according to the formula N=3×+2×+1×C where a is the percentage of intensely stained nuclei; In the percentage of moderately stained nuclei; With the percentage slightly positive nuclei characterized in that it further determined the distribution of receptors in tumor items breast cancer and the presence of areas with positive and negative expression, and with varying degrees of expression of the expression is considered heterogeneous, in the presence of a tumor uniform distribution of receptor trafficking is isimo from the intensity of staining the expression is considered homogeneous and homogeneous expression of steroid hormone receptors in the tumor predicts favorable course of the disease, and in the heterogeneous - negative. 2. The method according to claim 1, characterized in that, with varying degrees of expression is considered a combination of areas of the tumor with a strong and a weak level of expression or with severe and moderate.
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