Method for predicting the phases of stress states |
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IPC classes for russian patent Method for predicting the phases of stress states (RU 2288475):
  
Method for predicting the development of gastrointestinal tract paresis in patients with vertebral traumas / 2286089
In patients with vertebral traumas for about 2-3 h after lesion one should simultaneously determine the level of hormone cortisol, malonic dialdehyde (MDA) in blood serum, and, also, the value of total bioelectric activity (TBEA) of gastrointestinal tract (GIT) and at cortisol values being 981.7 nM/l and higher, and MDA values - 8.35 mM/l and higher and values of TBEA being 387.75 mcV and lower it is possible to predict the development of GIT paresis.
Method for predicting the development of stress reactions in early neonatal period in neonatals born in females with urogenital infection / 2284526
In serum of peripheral blood in pregnant women in trimester III of pregnancy period it is necessary to determine serotonin concentration. The flow of stress reactions in early neonatal period in neonatals should be predicted at serotonin level being above 0.1 mcg/ml. Application of the present method provides the opportunity for earlier prediction of the development of stress reactions in neonatals in early neonatal period.
Method of prognosing generalization of process in children and adolescents with osteal sarcoma / 2282195
Method provides for early prognosis of generalization disease due to most wide biological action of thyroidal hormones regulating principal stages of metabolic processes in body and influencing proliferation velocity and functions of nervous and immune systems, whose status, in turn, greatly determines state of antitumor résistance. Method is characterized by performing complex antitumor treatment and biochemical investigation and in patients undergone complex treatment with complete clinical and laboratory remission level of free fraction of thyroidal hormone triiodothironine is determined in blood. When this level lies within a range of 4.0 to 5.2 pmol/ml, preservation of remission during 6 to 24 month is forecasted and, at the level 5.8 to 7.4 pmol/ml, generalization of malignant process within period of 1 to 3 months is expected.
Method for diagnosing obliterated form of congenital adrenal gland cortex hyperplasia / 2279090
Method involves carrying out test with adrenocorticotropic. Blood levels of 11-desoxycortisol (S), cortisol (F), cortisone (E) and corticosterone (B) are determined by means of high performance liquid chromatography. Ratios of cortisol-to-cortisone levels (F/E) and to 11-desoxycortisol level (F/S) are calculated. Corticosterone level being lower than 17 ng/l and 11-desoxycortisol level being greater than 7.0 ng/l with F/E<5 and F/S<16, obliterated form of congenital adrenal gland cortex hyperplasia is to be diagnosed.
Method for predicting relapses in skin melanoma patients / 2275637
Method involves measuring daily estrone, estradiol and estriol excretion in women of reproductive age. Relation between estriol excretion and total amount of estrone and estradiol excretion after treatment and at control examination stage is calculated. The value being found to grow, relapse is predicted to occur in 11-12 months after the treatment.
Method for predicting bronchial asthma clinical development severity in adolescent patient at puberty transition time / 2273856
Method involves determining thyroid gland hormone of triiodothyronine (T3 in IU/l) and thyroxin (T4 in IU/l), pituitary hormones - thyrotropic hormone (TTH in IU/l), follicle-stimulating hormone (FSH in IU/l) and luteinizing hormone (LH in IU/l) concentration using radio isotope method and physical adolescent growth and development level with somatometric type being equal to 1 in microsomatic type cases, 2 in mesosomatic type cases and 3 in macrosomatic type cases. Discriminant function is determined in male adolescent case as D=19.535x(T3)+3.973x(T4)-27.38x(TTH)-11.028x(1.2 or 3)-307.473x(FSH)-17.585x(LH). The discriminant function value D>116.44 being the case, worsening bronchial asthma clinical course is predicted. D<116.44 being the case, bronchial improving asthma clinical course is predicted. Discriminant function is determined in female adolescent case as D=-4.908x(T3)-0.052x(T4)-0.355x(TTH)-3.166x(1.2 or 3)-0.769x(FSH)-0.144x(LH). The discriminant function value D>-24.23 being the case, worsening bronchial asthma clinical course is predicted. D<-24.23 being the case, improving bronchial asthma clinical course is predicted.
Method for predicting powerless labor in pregnant nodular goiter surgically treated on goiter occasion during pregnancy under medical supervision / 2273456
Method involves determining thyroid gland node diameter and thyrotropic hormone by applying ultrasonic examination approach. Histological examination is carried out with conclusion concerning morphologic nature being obtained like nodular colloid proliferating goiter or thyroid gland adenoma. Diagnostic index Σ is calculated from formula Σ=0.49*K1+0.07*K2-0.5*K3+1.76*K4-1.53, where K1 is the thyroid gland node diameter; K2 is the TTH concentration; K3 is the nodular colloid proliferating goiter index equal to 1 or 0; K4 is the thyroid gland adenoma index equal to 1 or 0. Diagnostic index Σ being less than zero, conclusions concerning powerless labor threat is to be drawn.
Method for predicting cholecystitis and cholelithiasis / 2263920
While diagnosing cholecystitis and cholelithiasis due to ultrasound testing one should additionally study blood plasma and bile to detect there the content of prostaglandins PGE2 and PGF2α. At PGE2/PGF2α ratio in blood plasma being equal to 6 and more, and, also, at decreased level of biliary cholecystokinin-pancreosimin by 38% and more, biliary PGE2 by 59% and more and increased level of biliary prostaglandin PGF2α by 5.9 times and more against the norm one should diagnose chlecystitis and cholelithiasis. The innovation enables to detect the above-mentioned diseases at earlier stage.
Method for predicting fetoplacental insufficiency in pregnant women with thyroid diseases / 2263919
Except detecting placental lactogen in blood serum one should study the content of alphafetoprotein. At placental lactogen content being below 75% against the norm and content of alphafetoprotein below 70% against the norm it is possible to conclude upon availability of fetoplacental insufficiency.
Method for predicting relapse of mammary cancer / 2263319
In the course of surveying in menopausal women after complex therapy one should state the development of mammary cancer at decreased ratio of estriol concentration to the sum of estrone and estradiol urinary concentrations from 1.68±0.23 in relapse-free patients up to 0.74±0.12 - in patients living without relapses for less than a year, up to 0.65±0.13 in patients living without relapse from 2 up to 6 years and up to 0.50±0.10 in patients with relapse-free period from 6 to 10 years. The innovation provides pre-clinical detection of mammary cancer relapse.
Method for predicting efficiency of interferonotherapy at chronic hepatitis b / 2287826
One should detect TNF-α content in blood serum and IFN-γ synthesis by mononuclear cells in vitro before and 4.5 h after the first injection of interferon-preparation for a patient. At detecting TNF-α content in blood serum before injection not exceeding 59.31 pg/ml, and at the increase of TNF-α level after the first injection of interferon- being above 46.1 % and IFN-γ synthesis being above 70.25% against initial data one should predict high efficiency of interferonotherapy in the course of a 6-mo-long interferonotherapy. At TNF-α content in blood serum being above 74.51 pg/ml and increased TNF-α values not more than 24.9%, and IFN-γ - not more than 70% or their decrease after the first injection of interferon- it is possible to predict the absence of virological response, that is insufficient efficiency of interferonotherapy. Application of the method suggested enables to predict the results of interferonotherapy in case of chronic viral hepatitis B just after the first injection of interferon- preparation.
Method for predicting the results of treating patients with chronic hepatitis c with interferon-alpha preparations / 2287825
One should detect the synthesis of IFN-γ and IL-4 with mononuclears of peripheral blood in vitro before and 4.5 h after introducing the first dosage of interferon-α preparation. At detecting more that 2-fold increase of the synthesis of IFN-γ and the ratio of IFN-γ/IL-4 synthesis after the first introduction of interferon-α preparation for the patients with CVH C it is possible to predict the development of virological response in the course of 24-wk interferonotherapy. In case of increased synthesis of IFN-γ and the ratio of IFN-γ/IL-4 synthesis after the first introduction of interferon-α being less than 2-fold - the development of virological response should not be expected. Application of the present innovation enables to predict the results of therapy in shorter terms after the first introduction of interferon-α preparation.
Method for predicting gestosis / 2287823
The present innovation deals with detecting the concentration of antibodies to neurospecific enolase (NSE) and gliofibrillo-acid protein (GFAP) in blood serum of pregnant women. The development of gestosis should be predicted at the level of anti-NSE-antibodies being above 0.6 mcg/ml or anti-GFAP-antibodies being above 0.9 mcg/ml. The innovation provides high information value and specificity of the method suggested to predict the development of gestosis.
 Glycoprotein vi as collagen recombinant receptor on platelets and its using in pharmaceutics / 2287580
Invention relates to DNA encoding glycoprotein VI (GP VI) used as a base for synthesis of recombinant GP VI by method of recombinant DNAs followed by its using for the development of pharmaceutical compositions possessing with capacity for inhibiting or blocking interaction of platelets with collagen. Using the invention provides preparing GP VI in the recombinant form and using this agent for therapeutic aims.
Method for differential diagnostics of chronic hepatitis and cirrhosis at hepatocerebral dystrophy / 2287165
In blood serum one should detect total constant for blood clotting, elasticity and time for clot formation, the content of ceruloplasmin. In case of decreased level of ceruloplasmin by 43-49%, increased total constant of blood clotting by 18-34% against the norm, at decreased clot elasticity by 12-24% against the norm and increased time for clot formation by 21-74% and more against the norm it is possible to state upon the presence of chronic hepatitis at hepatocerebral dystrophy. At decreased level of ceruloplasmin by 50% and more, increased total constant of blood clotting by 35% and more against the norm, decreased clot elasticity by 25% and more against the norm and increased time for clot formation by 75% and more against the norm it is possible to conclude upon the availability of cirrhosis in case of hepatocerebral dystrophy. Application of the method enables to increase accuracy of differential diagnostics of chronic hepatitis and cirrhosis in patients with hepatocerbral dystrophy.
Method for predicting the flow of rheumatoid uveitis / 2287164
In patients with rheumatoid uveitis in acute period of disease, that is within the 7th to the 10th d one should detect the concentration of tumor necrosis factor (TNF-α) concentration in blood serum. At its content being 150 pcg/ml and higher the flow of rheumatoid uveitis is considered to be favorable, and at concentration ranged 50-100 pcg/ml - unfavorable. The innovation increases the accuracy in predicting the severity degree of the flow of rheumatoid arthritis and provides the chance for conducting adequate medicinal therapy.
Method for early diagnostics of diabetic neuropathy in children / 2286578
One should detect the level of endothelin-1 (pcg/ml) and basic fibroblast growth factor (bFGF) (ng/ml) in patients with insulin-dependent diabetes mellitus in blood serum. At the ratio of endothelin-1 against bFGF being equal to 40 and higher it is possible to diagnose subclinical stage of diabetic neuropathy (DNP) in children. The innovation provides the opportunity for prescribing adequate therapy in due time and, thus, prevent the development of severe complications.
Method for predicting intracranial hemorrhages in neonatals / 2286577
It is necessary to determine neopterin concentration in spinal fluid and in blood serum. At values of neopterin concentration being 51.6±7.29 nM/l in blood serum and 16.3±3.80 nM/l in spinal fluid one should diagnose intracranial hemorrhage, moreover, one should apply the technique of immunoenzymatic assay that requires minimal quantity of biological liquids. The innovation is simple in implementation, requires small quantities of biological liquids that enables to apply it widely in resuscitation departments, neonatal pathology departments and in maternity hospitals.
 Method for carrying out early stage non-invasive diagnosis of bronchial remodeling processes in chronic obstructive lung disease in male cases / 2285264
Method involves determining transforming growth factor in male saliva β1 (TGF-β1) using immunoenzyme assay method with a set of standards for determining the TGF-β1. The TGF-β1 value growing 2, 4 and more times as large when compared to a value taken for healthy patient norm, bronchus remodeling is to be diagnosed. The value taken as healthy patient norm is equal to 403.88±102.58 selected as a result of forced expiration volume per 1 s comparison in healthy patients and bronchus biopsy results.
 Method for diagnosing metastases in lung root lymph node in pulmonary carcinoma cases / 2284040
Method involves determining lactate dehydrogenase activity and NADP-dependent glutamate dehydrogenase. Lactate dehydrogenase activity being equal to or greater than 12.50 mcU and NADP-dependent glutamate dehydrogenase activity being equal to or less than 0.25 mcU, no metastases in lung root lymph node in pulmonary carcinoma cases are thought to be available. Lactate dehydrogenase activity being less than 12.50 mcU and NADP-dependent glutamate dehydrogenase activity being greater than 0.25 mcU, the metastases are proved to be available.
 Method for predicting the character of bacterial keratitis flow / 2245553
In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula:
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FIELD: medicine, physiology, diagnostics. SUBSTANCE: in patient's blood one should detect the concentration of cortisol and fertile factors α2-microglobulin (AMGF), trophoblastic β1-1-glycoproteide (TBG) and α1-placental microglobulin (PAMG-1). The phases of stress states should be diagnosed by the coefficient calculated due to dividing the sum of AMGF and TBG concentrations by the sum of PAMG-1 and cortisol concentrations, moreover, the concentration should be expressed in % against average values in healthy persons. Coefficient's value being equal to 1.0-11 corresponds to the norm; at its value being 1.2-1.5 one should diagnose the phase of anxiety; at 0.7-0.9 - the phase of resistance; at 0.2-0.6 - the phase of exhaustion. The innovation provides the chance to program the flow of pathological process more accurately. EFFECT: higher accuracy and efficiency of diagnostics. 4 ex, 2 tbl
The invention relates to medicine, in particular to physiology, and can be used to diagnose disadaptive processes in humans in various pathological conditions. Known methods of diagnostics of violations disadaptive processes in humans in certain pathological conditions based on data Gsale (Selye,, 1982) on the inclusion of the hypothalamic-pituitary-adrenal system. The latter defines adaptive capacity of the organism to the action of the emergency stimulus, but it is not considered a deterrent mechanisms, absence or lack of inclusion is accompanied by the development of pathological processes leading to death of the organism (shock, consumption coagulopathy, and others) Analog is the definition of the degrees of stress conditions on the concentration of glucocorticoids (cortisol). The lack of similar in that only by changing the concentration of cortisol is difficult to diagnose the stage of stress as pronounced dependence on the nature of the stressor (Horizons P.D., 1981). The prototype is the way to diagnose the severity of stress conditions on the study of blood and the concentration of glucocorticoids (cortisone) and the associated status of the white blood cells (Garkavi LH, Cucina E.B., Ukolova M.A., 1990). However, dannysoar difficult to accounting, because the state of the leukocyte formula is treated by different doctors in their own way, which is reflected in the treatment of this group of patients. It is difficult to agree with the authors about the division of the stress response reaction training, response activation and directly the stress response. The concept of training finds a large spread in sports and here it is combined with reactions not weak, but on the contrary, medium and strong stressors. The disadvantage of this method is that it does not take into account the activity of reproductive factors more fully reflecting the functional state of the organism. The present invention is most accurately show the functional state of the organism by searching for indicators that objectively reflects the nature of the containment phase of the stress response in the development of stress conditions. This enables more accurate programming during the pathological process. The problem is solved due to the fact that the studied blood parameters reflecting the functional state of the organism and determine the concentration of glucocorticoids (cortisol) and reproductive factors (α2-microglobulin (AMHF), USSR β1-1-glikoproteid (TBG) and α1-placental microglobulin (PAMG-1)), diagnosing phase of stress conditions on the activity coefficient of sin is oxicash adaptation programs with regard to reproductive factors (CALPUFF), determined by the following dependencies:
where CAMGF- concentration α2-microglobulin fertility in %CTBGthe concentration of the USSR β1-1-glikoproteid in %CPAMG-1the concentration of placental α1-microglobulin in %, Withcortisolthe concentration of cortisol in %. The concentration or activity used to calculate the values expressed in % of the mean values in healthy people. The control were 20 healthy persons aged 18-25 years, CALPUFF have equal 1,07±0.01 fluctuations from 1.0 to 1.1. Allocation of reproductive factors (AMGF and TBG) promotes the inclusion of adaptation programs, resulting in less development phases of stress by maintaining homeostasis. Cortisol taken in this formula as an indicator of activity catatoxic adaptation programmes, which counteracts syntaxchecker adaptation programmes, as they operate reciprocal. This also contributes to and PAMG-1. The method is as follows: turning the patient to the doctor is given a full clinical assessment, and the degree of inclusion of adaptive programs with concentrations of reproductive factors. This ratio reflects the dynamic development of stress is eacli on activity levels of adaptation programs throughout the pathological process and is defined as follows. The patient is taken fasting blood from the cubital vein in the amount of 10 ml explores indicators of AMGF, TBG, PAMG-1 and cortisol on standardized methods. Then calculation of the activity coefficient syntaxchecker programs with regard to reproductive factors. The study was conducted on 95 individuals of both sexes from 25 to 55 years. Data on reproductive factors and cortisol were investigated in the dynamics of cold exposure and climacteric syndrome and shows their role in the development of the stress response in phases. Study of the development of the stress response during cold exposure was conducted on 80 individuals of both sexes from 25 to 35 years. Data on reproductive factors and cortisol are shown in table No. 1.
Indicator CALPUFF clearly indicated on the phase of stress depending on the strength and duration of the stimulus. In phase alarm indicator CALPUFF ranged from 1.2 to 1.5, the average of 1.36±0,03 by increasing the activity of reproductive factors, which was accompanied by the inclusion of adaptation programs in the first year of exposure to the stimulus. Further, during the development phase resistance, the concentration of reproductive factors decreased with increased activity of the adrenal cortex and is therefore a measure of CALPUFF decreased on average to 0.81±0,04 vary from 0.7 to 0.9. Further action chlorastrolite has led to the development stage of exhaustion (this phase usually occurs after 5 years long-term cold exposure or exposure to irritant moderate) with fluctuation of CALPUFF from 0.2 to 0.6, the average of 0.44±0,02, by reducing the activity of the adrenal cortex. Therefore, the coefficient CALPUFF can diagnostirovat the phase stress at cryogenic. Example 1. Patient C., 40 years old, came to the clinic 16.01.1997 year with complaints of pain in right hand and a small swelling, numbness. On the night 16.01.1997, there was frostbite while walking, when the temperature was -18°and her right hand was without gloves. Home brush warmed with warm water and the next day went to the polyclinic. Objective: the redness of the right hand with slight swelling. Pulsation of the radial artery is normal. Diagnosis: frostbite weak severity (I-II). Was taken blood from the left cubital vein on the study CALPUFF, which in this patient was 1.45 and corresponded to the stages of anxiety. Example No. 2. Patient M., 48 years old, came to the clinic 20.02.1997 year with complaints of pain in both feet with their swelling, numbness and a violation of their movement. 19.02.1997 year was fishing and wet my feet, the air temperature was -16°C. the House has warmed the feet with warm water and smeared with fat, but swelling is not reduced, and appeared vesicles with serous content. Objective: both feet and fingers swollen, swelling on the left reaches the lower third of the leg, blisters on fingers with serous content, the movement of the toes is limited due to swelling, sensitivity is reduced. Diagnosis: frostbite stop moderate (second degree). Taken blood from the cubital vein in the study CALPUFF. His figure was R the veins of 0.85, which corresponded to the stage of resistance and corresponded to moderate damage. Example No. 3. Patient A., 32 years old, came to the clinic 22.01.1995 year complaining of sharp pains in both hands and considerable swelling of both hands and fingers, restriction of movement, numbness. Frostbite has occurred in the night of 20 to 21 01.1995 year during operation, when the air temperature -25°C for 1.5 hours was without gloves. At home both hands warmed in hot water, smeared with fat, but later appeared bubbles with hemorrhagic content. Objective: both brushes and fingers dramatically swollen, swelling on the right reaches the elbow joint, the blisters on my fingers with hemorrhagic content, movements of the fingers is limited due to swelling and sensitivity is reduced. The pulsation of the radial artery saved. Diagnosis: frostbite brushes strong grade (III-IV degree). Taken blood from the cubital vein in the study CALPUFF. This index patient, 0.38 and corresponds to the stage of exhaustion, which is typical for a strong degree of damage, with a sharp prevalence catatoxic adaptation programmes and depression syntaxchecker mechanisms. Such shifts in the characteristics of the phases of stress syndrome occur in other pathological processes, such as menopausal syndrome. The data presented in table No. 2.
Example 4. Sick,, 52 years old, came to the clinic 21.02.2006 year with complaints of frequent flushing of blood to the head (five times daily), heart pain, palpitations, numbness, upper limbs, deterioration of health, strong sweating. Objective: increased blood pressure. The diagnosis of climacteric syndrome of moderate severity. Taken the blood of cubicals the th Vienna to study CALPUFF. His ratio was 0.88, which corresponds to the phase resistance and moderate severity. Therefore, the activity of adaptation programs, determining the resistance to extreme stimulus, depends on the concentration of reproductive factors. Thus, determining the ratio of CALPUFF or concentration of reproductive factors, we can talk about the diagnosis phase of stress conditions. The way to diagnose phases of stress conditions, including the study of blood, reflecting the functional state of the organism and the concentration of glucocorticoids, characterized in that to determine the concentration of cortisol and reproductive factors α2-microglobulin (AMHF), USSR β1-1-glikoproteid (TBG) and α1-placental microglobulin (PAMG-1), phase stressful conditions diagnosed by coefficient defined by the following dependencies:
where CAMGF- concentration α2- microglobulin fertility, %CTBGthe concentration of the USSR-β1-1-glikoproteid, %CPAMG-1the concentration of placental α1-microglobulin, %Ccortisolthe concentration of cortisol, %, and the concentration used to calculate the value of the expression is t in % of the mean values in healthy people, the value of the coefficient equal to 1.0-1.1, corresponds to the norm, when the value of the coefficient of 1.2 to 1.5 diagnosed anxiety phase, 0.7 to 0.9 - phase resistance of 0.2-0.6 - depletion phase.
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