Method for carrying out monitoring of diabetes mellitus patients state and neurological and vascular complications development |
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IPC classes for russian patent Method for carrying out monitoring of diabetes mellitus patients state and neurological and vascular complications development (RU 2291437):
 Method for predicting the phases of stress states / 2288475
In patient's blood one should detect the concentration of cortisol and fertile factors α2-microglobulin (AMGF), trophoblastic β1-1-glycoproteide (TBG) and α1-placental microglobulin (PAMG-1). The phases of stress states should be diagnosed by the coefficient calculated due to dividing the sum of AMGF and TBG concentrations by the sum of PAMG-1 and cortisol concentrations, moreover, the concentration should be expressed in % against average values in healthy persons. Coefficient's value being equal to 1.0-11 corresponds to the norm; at its value being 1.2-1.5 one should diagnose the phase of anxiety; at 0.7-0.9 - the phase of resistance; at 0.2-0.6 - the phase of exhaustion. The innovation provides the chance to program the flow of pathological process more accurately.
Method for predicting the development of gastrointestinal tract paresis in patients with vertebral traumas / 2286089
In patients with vertebral traumas for about 2-3 h after lesion one should simultaneously determine the level of hormone cortisol, malonic dialdehyde (MDA) in blood serum, and, also, the value of total bioelectric activity (TBEA) of gastrointestinal tract (GIT) and at cortisol values being 981.7 nM/l and higher, and MDA values - 8.35 mM/l and higher and values of TBEA being 387.75 mcV and lower it is possible to predict the development of GIT paresis.
Method for predicting the development of stress reactions in early neonatal period in neonatals born in females with urogenital infection / 2284526
In serum of peripheral blood in pregnant women in trimester III of pregnancy period it is necessary to determine serotonin concentration. The flow of stress reactions in early neonatal period in neonatals should be predicted at serotonin level being above 0.1 mcg/ml. Application of the present method provides the opportunity for earlier prediction of the development of stress reactions in neonatals in early neonatal period.
Method of prognosing generalization of process in children and adolescents with osteal sarcoma / 2282195
Method provides for early prognosis of generalization disease due to most wide biological action of thyroidal hormones regulating principal stages of metabolic processes in body and influencing proliferation velocity and functions of nervous and immune systems, whose status, in turn, greatly determines state of antitumor résistance. Method is characterized by performing complex antitumor treatment and biochemical investigation and in patients undergone complex treatment with complete clinical and laboratory remission level of free fraction of thyroidal hormone triiodothironine is determined in blood. When this level lies within a range of 4.0 to 5.2 pmol/ml, preservation of remission during 6 to 24 month is forecasted and, at the level 5.8 to 7.4 pmol/ml, generalization of malignant process within period of 1 to 3 months is expected.
Method for diagnosing obliterated form of congenital adrenal gland cortex hyperplasia / 2279090
Method involves carrying out test with adrenocorticotropic. Blood levels of 11-desoxycortisol (S), cortisol (F), cortisone (E) and corticosterone (B) are determined by means of high performance liquid chromatography. Ratios of cortisol-to-cortisone levels (F/E) and to 11-desoxycortisol level (F/S) are calculated. Corticosterone level being lower than 17 ng/l and 11-desoxycortisol level being greater than 7.0 ng/l with F/E<5 and F/S<16, obliterated form of congenital adrenal gland cortex hyperplasia is to be diagnosed.
Method for predicting relapses in skin melanoma patients / 2275637
Method involves measuring daily estrone, estradiol and estriol excretion in women of reproductive age. Relation between estriol excretion and total amount of estrone and estradiol excretion after treatment and at control examination stage is calculated. The value being found to grow, relapse is predicted to occur in 11-12 months after the treatment.
Method for predicting bronchial asthma clinical development severity in adolescent patient at puberty transition time / 2273856
Method involves determining thyroid gland hormone of triiodothyronine (T3 in IU/l) and thyroxin (T4 in IU/l), pituitary hormones - thyrotropic hormone (TTH in IU/l), follicle-stimulating hormone (FSH in IU/l) and luteinizing hormone (LH in IU/l) concentration using radio isotope method and physical adolescent growth and development level with somatometric type being equal to 1 in microsomatic type cases, 2 in mesosomatic type cases and 3 in macrosomatic type cases. Discriminant function is determined in male adolescent case as D=19.535x(T3)+3.973x(T4)-27.38x(TTH)-11.028x(1.2 or 3)-307.473x(FSH)-17.585x(LH). The discriminant function value D>116.44 being the case, worsening bronchial asthma clinical course is predicted. D<116.44 being the case, bronchial improving asthma clinical course is predicted. Discriminant function is determined in female adolescent case as D=-4.908x(T3)-0.052x(T4)-0.355x(TTH)-3.166x(1.2 or 3)-0.769x(FSH)-0.144x(LH). The discriminant function value D>-24.23 being the case, worsening bronchial asthma clinical course is predicted. D<-24.23 being the case, improving bronchial asthma clinical course is predicted.
Method for predicting powerless labor in pregnant nodular goiter surgically treated on goiter occasion during pregnancy under medical supervision / 2273456
Method involves determining thyroid gland node diameter and thyrotropic hormone by applying ultrasonic examination approach. Histological examination is carried out with conclusion concerning morphologic nature being obtained like nodular colloid proliferating goiter or thyroid gland adenoma. Diagnostic index Σ is calculated from formula Σ=0.49*K1+0.07*K2-0.5*K3+1.76*K4-1.53, where K1 is the thyroid gland node diameter; K2 is the TTH concentration; K3 is the nodular colloid proliferating goiter index equal to 1 or 0; K4 is the thyroid gland adenoma index equal to 1 or 0. Diagnostic index Σ being less than zero, conclusions concerning powerless labor threat is to be drawn.
Method for predicting cholecystitis and cholelithiasis / 2263920
While diagnosing cholecystitis and cholelithiasis due to ultrasound testing one should additionally study blood plasma and bile to detect there the content of prostaglandins PGE2 and PGF2α. At PGE2/PGF2α ratio in blood plasma being equal to 6 and more, and, also, at decreased level of biliary cholecystokinin-pancreosimin by 38% and more, biliary PGE2 by 59% and more and increased level of biliary prostaglandin PGF2α by 5.9 times and more against the norm one should diagnose chlecystitis and cholelithiasis. The innovation enables to detect the above-mentioned diseases at earlier stage.
Method for predicting fetoplacental insufficiency in pregnant women with thyroid diseases / 2263919
Except detecting placental lactogen in blood serum one should study the content of alphafetoprotein. At placental lactogen content being below 75% against the norm and content of alphafetoprotein below 70% against the norm it is possible to conclude upon availability of fetoplacental insufficiency.
Method for evaluating inflammatory process activity at psoriasis / 2290645
The present innovation deals with laboratory methods for studying skin diseases by detecting the content of inflammatory process markers in blood serum at the moment of disease exacerbation and in the end of therapy. As markers of inflammatory process one should apply alpha-1 acid glycoprotein and nitrogen oxide. At decreased content of acid glycoprotein in blood serum ranged 1.19-1.21 g/l up to 0.78-0.82, and as for nitrogen oxide ranged 44.5-54.4 mcM/l up to 24-29.2 mcM/l one should evaluate psoriasis activity to be high. At decreased content of alpha-1 acid glycoprotein ranged 1-1.04 g/l - up to 0.76-0.8 g/l, and nitrogen oxide ranged 37.9-43.5 mcM/l up to 20.6-27.4 mcM/l the psoriasis activity should be evaluated as low. The innovation provides higher significance of diagnostics, decreases labor intensity and period for carrying out the experiments mentioned that enables to develop a number of individual curative procedures for every patient.
 Method for predicting cerebral disorders in neonatals in mothers from high-risk group / 2290644
One should sample the waters during the period of delivery in pregnant women from the risk group at verified urogenital infection and/or the presence of fetoplacental insufficiency to detect the value of TNF-α and neopterin. At TNF-α being above 88.1±7.9 pg/ml and neopterin being above 17.3±1.6 ng/ml one should predict hypoxic-ischemic lesion of neonatals' CNS. The innovation enables to indicate earlier curative procedures.
Method for differential diagnosis of perthes disease stages in children and youngsters / 2289818
Method involves inspection of children and youngsters suffering from Perthes disease by convention methods that involve analysis of clinical symptoms of disease and results of the roentgenology study. In case of difficulties in assay of stages of pathological process method involves taking off venous blood sample, separation of red cells wherein activity of enzyme superoxide dismutase is determined. At value of activity of superoxide dismutase above 153.9 U/g Hb/min II-III stages of Perthes disease are diagnosed and at value of this index lower 142.6 U/g Hb/min IV-V stages of Perthes disease are diagnosed. Using the method allows enhancing safety of differential diagnosis of II-III and IV-V stages of Perthes disease.
Method for predicting the phases of stress states / 2288475
In patient's blood one should detect the concentration of cortisol and fertile factors α2-microglobulin (AMGF), trophoblastic β1-1-glycoproteide (TBG) and α1-placental microglobulin (PAMG-1). The phases of stress states should be diagnosed by the coefficient calculated due to dividing the sum of AMGF and TBG concentrations by the sum of PAMG-1 and cortisol concentrations, moreover, the concentration should be expressed in % against average values in healthy persons. Coefficient's value being equal to 1.0-11 corresponds to the norm; at its value being 1.2-1.5 one should diagnose the phase of anxiety; at 0.7-0.9 - the phase of resistance; at 0.2-0.6 - the phase of exhaustion. The innovation provides the chance to program the flow of pathological process more accurately.
Method for predicting efficiency of interferonotherapy at chronic hepatitis b / 2287826
One should detect TNF-α content in blood serum and IFN-γ synthesis by mononuclear cells in vitro before and 4.5 h after the first injection of interferon-preparation for a patient. At detecting TNF-α content in blood serum before injection not exceeding 59.31 pg/ml, and at the increase of TNF-α level after the first injection of interferon- being above 46.1 % and IFN-γ synthesis being above 70.25% against initial data one should predict high efficiency of interferonotherapy in the course of a 6-mo-long interferonotherapy. At TNF-α content in blood serum being above 74.51 pg/ml and increased TNF-α values not more than 24.9%, and IFN-γ - not more than 70% or their decrease after the first injection of interferon- it is possible to predict the absence of virological response, that is insufficient efficiency of interferonotherapy. Application of the method suggested enables to predict the results of interferonotherapy in case of chronic viral hepatitis B just after the first injection of interferon- preparation.
Method for predicting the results of treating patients with chronic hepatitis c with interferon-alpha preparations / 2287825
One should detect the synthesis of IFN-γ and IL-4 with mononuclears of peripheral blood in vitro before and 4.5 h after introducing the first dosage of interferon-α preparation. At detecting more that 2-fold increase of the synthesis of IFN-γ and the ratio of IFN-γ/IL-4 synthesis after the first introduction of interferon-α preparation for the patients with CVH C it is possible to predict the development of virological response in the course of 24-wk interferonotherapy. In case of increased synthesis of IFN-γ and the ratio of IFN-γ/IL-4 synthesis after the first introduction of interferon-α being less than 2-fold - the development of virological response should not be expected. Application of the present innovation enables to predict the results of therapy in shorter terms after the first introduction of interferon-α preparation.
Method for predicting gestosis / 2287823
The present innovation deals with detecting the concentration of antibodies to neurospecific enolase (NSE) and gliofibrillo-acid protein (GFAP) in blood serum of pregnant women. The development of gestosis should be predicted at the level of anti-NSE-antibodies being above 0.6 mcg/ml or anti-GFAP-antibodies being above 0.9 mcg/ml. The innovation provides high information value and specificity of the method suggested to predict the development of gestosis.
 Glycoprotein vi as collagen recombinant receptor on platelets and its using in pharmaceutics / 2287580
Invention relates to DNA encoding glycoprotein VI (GP VI) used as a base for synthesis of recombinant GP VI by method of recombinant DNAs followed by its using for the development of pharmaceutical compositions possessing with capacity for inhibiting or blocking interaction of platelets with collagen. Using the invention provides preparing GP VI in the recombinant form and using this agent for therapeutic aims.
Method for differential diagnostics of chronic hepatitis and cirrhosis at hepatocerebral dystrophy / 2287165
In blood serum one should detect total constant for blood clotting, elasticity and time for clot formation, the content of ceruloplasmin. In case of decreased level of ceruloplasmin by 43-49%, increased total constant of blood clotting by 18-34% against the norm, at decreased clot elasticity by 12-24% against the norm and increased time for clot formation by 21-74% and more against the norm it is possible to state upon the presence of chronic hepatitis at hepatocerebral dystrophy. At decreased level of ceruloplasmin by 50% and more, increased total constant of blood clotting by 35% and more against the norm, decreased clot elasticity by 25% and more against the norm and increased time for clot formation by 75% and more against the norm it is possible to conclude upon the availability of cirrhosis in case of hepatocerebral dystrophy. Application of the method enables to increase accuracy of differential diagnostics of chronic hepatitis and cirrhosis in patients with hepatocerbral dystrophy.
Method for predicting the flow of rheumatoid uveitis / 2287164
In patients with rheumatoid uveitis in acute period of disease, that is within the 7th to the 10th d one should detect the concentration of tumor necrosis factor (TNF-α) concentration in blood serum. At its content being 150 pcg/ml and higher the flow of rheumatoid uveitis is considered to be favorable, and at concentration ranged 50-100 pcg/ml - unfavorable. The innovation increases the accuracy in predicting the severity degree of the flow of rheumatoid arthritis and provides the chance for conducting adequate medicinal therapy.
 Method for predicting the flow of nervous-psychic diseases / 2258934
One should test blood serumal samples in immunoenzymatic assay by applying a test system ELI-N-1, the components of which are being antigens of nervous tissue or their immunochemical analogs specifically binding antibodies with tendency to antigens of nervous tissue, and, also, idiotypical antibodies to them, or their variable parts. Prediction should be performed according to the level of idiotypical and anti-idiotypical autoantibodies to antigens of nervous tissue and, also, according to their ratio. Thus, a new test system has been elaborated that enables to predict the flow of available nervous-psychic diseases.
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FIELD: medicine. SUBSTANCE: method involves determining immune reactivity of blood serum with respect to insulin, to anti-insulin antibodies or their antigen-binding fragments, to anti-anti-insulin antibodies binding the antibodies to insulin and antigens to growth factor or their antigen-binding fragments and ANCA antigen. Blood serum immunoreactivity increase with respect to parameters under measurement relative to norm is used for determining diabetes mellitus neurological and vascular complications development. EFFECT: high accuracy of monitoring.
The invention relates to medicine, in particular to therapy and endocrinology, and may find application in assessing the effectiveness of therapy of diabetes mellitus type 1 and 2. It is known that the immune system of a healthy person constantly produces natural regulatory autoantibodies (auto-AB) to any of the antigenic components of its own body, including auto-antibodies to components of the cells of the pancreas and insulin receptors (Poletaev A.B. and other Regulatory metasystem, M.: Medicine, 2002). Synthesis of auto-antibodies in a healthy body is maintained within certain limits, required to run past certain regulatory functions, and their excessive as well as insufficient production can lead to the development of pathological conditions, including insulin-dependent diabetes mellitus (DM type 1) and non-insulin dependent diabetes mellitus (DM type 2), as well as vascular and neurological complications of the underlying disease, often leading to permanent disability. This has been the basis for the development of the proposed method suitable for diagnostic and prognostic assessment of levels of a number of auto-antibodies, the content of which reflects the level of autoimmunogenic to insulinsecreting cells of the pancreas in DM-1, peripheral insulin receptors in DM-2, and ASU is estolate control over the development of the main complications of diabetes (cardiovascular and neurological). The generally accepted method for the diagnosis of diabetes and monitoring of their condition is the determination of the level of sugar in the blood (Dev, Ussales. "Clinical laboratory diagnostics" Hippocrates" SPb, 1997, str). The disadvantage of this known method is its relatively low reliability and the impossibility of monitoring the development of major vascular and neurological complications that accompany diabetes. The objective of the invention is a method of monitoring the condition of patients with diabetes mellitus and the development of neurological and vascular complications, allowing more objectively judge the progression of diabetes and the development of neurological and vascular complications. The problem is solved by the method lies in the fact that determine the immunoreactivity of serum in relation to insulin, to antiinsulin antibody (the binding of antibodies to insulin receptors) or their antigennegative fragments, intentionality antibodies that bind antibodies to insulin and antibodies to nerve growth factor (antiidiotype antiinsulin antibodies) or antigennegative fragments and the antigen of ANCA and with the increase in the immunoreactivity for 6-15% in relation to insulin and/or antiinsulin antibodies or their antigennegative fragments conclude about the mind is adopted progression of diabetes, when used simultaneously in the same degree of increase of immunoreactivity against intentionalism antibodies that bind antibodies to insulin and antibodies to nerve growth factor or antigennegative fragments conclude about moderate progression of diabetic neuropathy, and with a simultaneous increase in the same degree of immunoreactivity against ANCA conclude diabetic angiopathy, and at a higher immunoreactivity in relation to the listed reagents conclude a high degree of progression of diabetes and its neurological and vascular complications. The practical implementation of the method includes the following stages: I. Sorption on a standard 96-well polystyrene plates to ELISA (a) insulin b) molecules idiotypical antiinsulin antibodies (or fragments) - immunochemical analogues insulin receptors; b) antiidiotypic antibodies (intential), specifically interacts with antibodies to insulin; C) anionic proteins in the cytoplasm of neutrophils (ANCA); II. The application of the tested serum samples of patients and reference reference sera obtained from healthy individuals; III. Identifying formed antigenetically complexes using conjugates secondary (antivitamin) immunoglobulins labeled with horseradish peroxidase or ins is an enzyme; IV. The manifestation of the reaction in the wells with substrate-Chromogen; V. comparison of the intensity of the reaction in the wells containing test serum to the wells containing the reference serum. For analysis ELI-DIA-Test takes about 0,03 ml of the blood serum of the subject (e.g., obtained from the finger pad). THE BASIC COMPONENTS USED FOR THE PROPOSED METHOD (TEST SYSTEM ELI-DIA-TEST). 1. The paper uses the commercial preparation of insulin. 2. Getting antiinsulin antibodies or their antigenspecific fragments was performed using standard procedures immunoaffinity chromatography as described previously (Poletaev A.B. and others J. Neuroimmunology, 2003, 1, 1, 11-17). 3. Getting intentionality antibodies or their antigenspecific fragments was performed using standard procedures immunoaffinity chromatography as described previously (Poletaev A.B. and others J. Neuroimmunology, 2003, 1, 1, 11-17). 4. Getting ANCA antigen was carried out as follows: from whole human blood were isolated fraction of neutrophils, were extracted using a neutral buffer solution (for example, Tris-chloride), the extract was passed through the anion-exchange column (for example, DEAE-cellulose), who contacted the material was suirable salt solution (for example, 1 M chloride intothree is) and used in the work. METHODOLOGY FOR INDIRECT ELISA. 1. For sorption on standard 96-well flat-bottomed plates to assay prepared solutions of each component of the test system in carbonate buffer pH 9-10. The prepared solutions were made in separate wells and incubated for 1-24 hours 2. After incubation, washed tablets wash buffer (for example, 0.15 M NaCl with 0.05% tween-20) and tested sera; to do this: (a) in separate vials or trays for ELISA were prepared by dilution of sera (1:200) in wash buffer, b) made of diluted serum in wells, C) the plates were incubated overnight at +4°or 1-3 h at +37...+39°C g) washed tablets and showed the reaction using standard techniques, using a conjugate of horseradish peroxidase (or other enzyme) with antibodies to immunoglobulins IgG man and a solution of substrate-Chromogen (e.g., 0.01-0.05% o-phenylenediamine with 0.001-0.01% hydrogen peroxide in 0.01-0.05 M citrate-phosphate buffer in case of application of peroxidase conjugates). 3. Incubated plates in the dark for 10-30 min at room temperature. 4. Upon reaching the optimal level of staining (usually 10-20 min incubation in the dark) reaction stopped by the addition of 0.2-0.4 M tartaric acid. 5. Check the results: intensives the e staining assessed using enzyme immunoassay analyzer (ELISA-reader) of any model. 6. Data processing: on the obtained values of optical density of holes calculate the relative intensity of the reaction was tested sera with each of the components of the test system ELI-DIA-Test relative to the reaction control sera with the same antigen minus 100 units and expressed in arbitrary units. To calculate using the formula:
where R(a) is the optical density of the analyzed blood serum in the wells with antigen-1; R(AGW) is the optical density of the analyzed blood serum in the wells with antigen-n; R(K1...KP) is the optical density of the control serum in the wells with antigen 1...n. 7. The interpretation of these data: a) if the intensity of the response of the test serum with any of the components of the test system did not go beyond +5% relative to the reaction serum reference, this serum is referred to as the 1st classification group (those without progression of the underlying disease and no signs of complications), b) if the intensity of the response of the test serum with any of the components outside the group-1 but does not exceed +15% relative to the reaction serum reference, this serum is dated to the 2nd classification group or a group of moderate rejected the th (persons with progression of diabetes and/or complications of moderate severity), C) if the intensity of the response of the test serum with any of the components beyond the 1st and 2nd groups, this serum is referred to as the 3rd classification group or a group expressed deviations (persons with a high risk of rapid progression of the underlying disease and/or its complications), g) the likelihood of progression of DM-1 proportional to the degree of abnormalities in the content of auto-antibodies to insulin, d) the probability of progression of DM-2 proportional to the degree of abnormalities in the content of auto-antibodies to insulin receptors, detected by the reaction in the wells with adsorbed antiinsulin antibodies (idiotypical) or their fragments, (e) the likelihood of progression of diabetic neuropathy proportional to the degree of abnormalities in the content of antibodies cross-reacting with insulin and nerve growth factor detected by the reaction in the wells with the adsorbed anti-antiinsulin antibodies (antiadiotipiceskih) or their fragments, W) the probability of progression of diabetic angiopathy proportional to the degree of abnormalities in the content of antibodies reactive with the antigen of ANCA, C) dynamic changes in the content of the corresponding auto-antibodies can be judged positive or negative trends in the state of the patient, for example, on the background of conduct the treatment. It should be noted that the analysis is performed in vitro without any harm to the subject. EXAMPLES EXAMPLE 1 Surveyed 1 The results of the analyses ELI-DIA-Test (immunoreactivity in % of the reaction control sera):
Diagnosis: newly diagnosed diabetes mellitus type-2; disease duration of approximately 6 months. Clinical signs of complications were found. EXAMPLE 2 Surveyed 2 The results of the analyses ELI-DIA-Test (immunoreactivity in % of the reaction control sera):
Diagnosis: diabetes mellitus type-1 with signs of insulin resistance; disease duration of about 5 years. Clinical signs of diabetic neuropathy. EXAMPLE 3 Surveyed 3 The results of the analyses ELI-DIA-Test (immunoreactivity in % of the reaction control savoro the key):
Diagnosis: diabetes mellitus type-2 with signs of insulin resistance (combined type 1+2); disease duration of about 8 years. Clinical signs of diabetic angiopathy with changes in the fundus, the signs of neuropathy. EXAMPLE 4 Surveyed 4 The results of the analyses ELI-DIA-Test (immunoreactivity in % of the reaction control sera):
Diagnosis: diabetes mellitus type-1, state of decompensation; signs of insulin resistance; disease duration of about 3 years. Clinical signs of diabetic neuropathy. Exacerbation of chronic infectious process. EXAMPLE 5 Surveyed 5 The results of the analyses ELI-DIA-Test (immunoreactivity in % of the reaction control sera):
Diagnosis: diabetes mellitus type-2, compensated, with no signs of autoimmune pathogenetic component; disease duration of about 10 years. Clinical signs of diabetic angiopathy. In contrast to the above of the prototype method the new method allows to: 1) to register pathological changes in serum levels of auto-antibodies that interact with insulin (their changes is a characteristic feature of SD-1), 2) to register pathological changes in serum levels of auto-antibodies that interact with membrane insulin receptors (their changes is a characteristic feature of the SD-2), 3) to register pathological changes in serum levels of auto-antibodies, changes which precede and accompany the development of major vascular and neurological complications of diabetes like type 1 and type 2 diabetic angiopathy, diabetic neuropathy), 4) to register pathological changes, dangerous for those suffering from diabetes, but to give a forecast of the development of the underlying disease in all persons with diabetes. Method of monitoring patients with diabetes mellitus, zakluchalos what is to determine the immunoreactivity of serum in relation to insulin, to antiinsulin antibodies or their antigennegative fragments, to intentionality antibodies that bind insulin antibodies and antibodies to the growth factor, or antigennegative fragments and the antigen of ANCA and with the increase in the immunoreactivity for 6-15% of the norm in relation to insulin and/or antiinsulin antibodies or their antigennegative fragments conclude about moderate progression of diabetes, when used simultaneously in the same degree of increase of immunoreactivity against intentionalism antibody or antigennegative fragments conclude about moderate progression of diabetic neuropathy, and with a simultaneous increase in such the same degree of immunoreactivity against ANCA conclude diabetic angiopathy, and at a higher immunoreactivity in relation to the listed reagents conclude a high degree of progression of diabetes and its neurological and vascular complications.
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