Method for differential diagnostics of chronic hepatitis and cirrhosis at hepatocerebral dystrophy

FIELD: medicine, hepatology.

SUBSTANCE: in blood serum one should detect total constant for blood clotting, elasticity and time for clot formation, the content of ceruloplasmin. In case of decreased level of ceruloplasmin by 43-49%, increased total constant of blood clotting by 18-34% against the norm, at decreased clot elasticity by 12-24% against the norm and increased time for clot formation by 21-74% and more against the norm it is possible to state upon the presence of chronic hepatitis at hepatocerebral dystrophy. At decreased level of ceruloplasmin by 50% and more, increased total constant of blood clotting by 35% and more against the norm, decreased clot elasticity by 25% and more against the norm and increased time for clot formation by 75% and more against the norm it is possible to conclude upon the availability of cirrhosis in case of hepatocerebral dystrophy. Application of the method enables to increase accuracy of differential diagnostics of chronic hepatitis and cirrhosis in patients with hepatocerbral dystrophy.

EFFECT: higher accuracy and efficiency of diagnostics.

4 ex

 

The invention relates to medicine and can be used as a method of differential diagnosis of chronic hepatitis and cirrhosis of the liver, with Reye dystrophy.

A known method for the diagnosis of Reye dystrophy by determining copper excretion with bile adopted for similar [1].

A known method of differential diagnosis of chronic hepatitis and early cirrhosis adopted for the prototype [2], which includes the determination of indices of coagulation, a transaminase activity, phosphatase, bilirubin, total protein and its fractions, sediment samples, subpopulations of T - and b-lymphocytes, the level of glucagon in plasma. The difference of the proposed method for the identification of groups of patients, namely patients with Reye dystrophy; serum define the content of ceruloplasmin, total constant coagulation, elasticity and the formation of a blood clot; determination of ceruloplasmin carried out by adding a solution of paraphenylenediamine, inquira for 15 min at 37 ° °With, then adding a solution of sodium azide, stirring and cooling.

In addition, the accuracy of determination of copper and ceruloplasmin for the differential diagnosis of chronic hepatitis and cirrhosis of the liver, with Reye dystrophy according to FR the type insufficient, since the decrease in the content of copper and ceruloplasmin observed in nephrotic syndrome, burns, chronic ischemic heart disease, some iron-deficiency anemia.

The aim of the invention is to improve the accuracy of diagnosis.

The technical result is achieved that determine the constant blood clotting, elasticity and the formation of the clot, the content of ceruloplasmin, and by decreasing the level of ceruloplasmin on 43-49% and the increase in the overall constant coagulation 18-34% compared with normal and decreased elasticity of the bunch for 12-24% in comparison with the norm, increasing the time of formation of a blood clot on 21-74% compared to the norm is judged on the presence of chronic hepatitis with Reye dystrophy; and by decreasing the level of ceruloplasmin by 50% or more and the increase in the overall constant coagulation by 35% or more compared to the norm and the decreased elasticity of the bunch of 25% or more compared to the norm, increasing the time of formation of a blood clot on 75% or more compared to the norm is judged on the presence of cirrhosis with Reye dystrophy.

The method is implemented as follows.

When entering patient presents with complaints of weakness, nosebleeds, hand tremor, change in gait, memory loss. Weakness, nasal bleeding and pain in promodeler, enlargement of the liver, moderate hepatomegaly appeared a few years ago. Was observed for chronic hepatitis unspecified etiology.

Admission: General state of moderate severity. It is difficult, aphonia. Thinking and reaction slowed down, comes into contact with the work, monosyllabic answers. Gait disturbed. The skin is a normal color. Auscultation of the vesicular breathing, wheezing no. Heart sounds clean, regular rhythm. Blood pressure and heart rate is within normal limits. The abdomen is soft, painful to palpation in the right hypochondrium. The liver stands out from the edge of the costal arch. The spleen is enlarged. Ascites and edema not.

The results of the survey:

Urinalysis - without a pathology, the level of immunoglobulins in the blood serum IgA, IgG, IgM - above normal, the levels of cytokines (IFSγ, IL-12, TNF-α) - above normal

Hemoglobin, erythrocytes, leukocytes reduced; WBC - within normal limits; thrombocytopenia, prothrombin decreased, glucose is within the normal range;

Determine the constant blood clotting, elasticity and the formation of the clot, the content of ceruloplasmin. At lower levels of ceruloplasmin on 43-49% and the increase in the overall constant coagulation 18-34% compared with normal and decreased elasticity of the bunch for 12-24% in comparison with the norm, increasing the time of formation of blood Shust is and 21-74% compared to the norm is judged on the presence of chronic hepatitis with Reye dystrophy; and by decreasing the level of ceruloplasmin by 50% or more and the increase in the overall constant coagulation by 35% and more in comparison with normal and decreased elasticity of the bunch of 25% or more compared to the norm, increasing the time of formation of a blood clot on 75% or more compared to the norm is judged on the presence of cirrhosis.

Determination of ceruloplasmin carried out by adding a solution of paraphenylenediamine, inquira for 15 min at 37°C, add a solution of sodium azide, mixing cool.

Consultation: Identified the ring of Kayser-Fleischer.

Ultrasound: Liver - structure parenchyma is homogeneous, echo uniformly increased vascular drawing is saved. The portal vein is not extended (12 mm). Intrahepatic bile ducts are not dilated. Gall bladder: Dimensions slightly increased, the echo wall raised around the gallbladder hypoechoic area. The spleen is enlarged. The splenic vein extended collaterals.

Conclusion: Signs of diffuse liver disease. Portal hypertension. Marked splenomegaly.

EGD: On the mucosal surface of the esophagus indicates the presence of varicose veins. The mucosa is hyperemic, erosive changes it is not revealed. During insertion of the fiberscope, there is a small contact bleeding of mucous terminal is tion of the esophagus.

Conclusion: Varicose veins of the esophagus.

Liver biopsy was not performed because of the risk of bleeding.

The diagnosis of Reye dystrophy with primary liver disease and brain structures. Cirrhosis of the liver active, asthma. Hepatic failure class With child-Pugh. Varicose veins of the esophagus 4 tbsp. Splenomegaly with symptoms of hypersplenism. Pancytopenia.

Conclusion: Given the condition of the patient, expressed pancytopenia patient assigned pathogenetic treatment kuprenilom, at impossibility of pathogenetic therapy for the underlying disease, patients are encouraged liver transplantation.

The method further explain examples of its implementation.

Example 1

Patient P s, 20 years history was admitted to the Department of chronic liver disease with complaints of weakness, nosebleeds, pain in the right hypochondrium, which appeared about 7 years ago. Was observed for chronic hepatitis unspecified etiology.

Admission: General state of moderate severity. It is difficult, thinking and reaction slowed down. the skin is a normal color. Auscultation of the vesicular breathing, wheezing no. Heart sounds clean, regular rhythm. Heart rate is 74 per minute, BP-110/70 mm Hg Abdomen is soft, painful to palpation in blastophaga hypochondrium. Liver at 1.5 cm stands from under the edge of the costal arch. Spleen occupies the entire left half of the abdomen. Ascites and edema not.

The results of the survey:

Hemoglobin 10.1 g/DL, erythrocytes - 3,6·109leukocyte - 0,4·109thousand, eosinophils - 3, p/I - 1, I - 60, lymphocytes - 26, monocytes - 8, platelet - 122·109; ESR - 12 mm/HR, glucose - 3.6 mmol/l, bilirubin and 17.9 mmol/l, cholesterol - 2.1 mmol/l, alkaline phosphatase - 218 IU/l, γ-STES-44 IU/l, AST - 41 IU/l, Alt - 26 IU/l, total protein - 72 g/l, albumin - 32 g/l, prothrombin 55%, amylase - 160 IU/l, lipase - 137 IU/l, urea - 5,0 mmol/l, creatinine - 65.3 mmol/l, iron - 14,8 mg %.

Determination of ceruloplasmin carried out by adding a solution of paraphenylenediamine, inquira for 15 min at 37°C, add a solution of sodium azide, mixing cool.

Urinalysis: UD. weight - 1012, protein - no sugar - no, leukocytes - a single in sight, erythrocytes - 0-1 in the field of view.

The content of IgA - 3.0 g/l (control, 0.3 g/l), IgG - of 21.2 g/l (control 2.1 g/l), IgM - 3.8 g/l (control, 0.3 g/l), PCR HBV, HCV, HGV, HTTV neg.

The concentration of cytokines: ifγ - 260 PG/ml, IL-12 - 860 PG/ml, TNF-α - 780 PG/ml (normal is 50 PG/l).

Consultation: Identified the ring of Kayser-Fleischer.

Ultrasound: Liver - structure parenchyma is homogeneous, echo uniformly increased vascular pattern of conservation of the yen. The portal vein is not extended (12 mm). Intrahepatic bile ducts are not dilated. Gall bladder: Sizes 7×2.9 cm, echo wall raised around the gallbladder hypoechoic area 1,3-1,2 see Splenomegaly 21,2×12,0 see the Splenic vein extended (16 mm) with collaterals.

Conclusion: Signs of diffuse liver disease. Portal hypertension. Marked splenomegaly.

EGD: hyperemic Mucosa of the esophagus, without erosions on the surface of giant varicose expanded veins of an esophagus, completely fill the lumen of the esophagus. The fiberscope cautiously held in the stomach and duodenum. During insertion of the fiberscope, there is a small contact bleeding of the mucosa of the terminal esophagus.

Conclusion: Varicose veins of the esophagus 4 degrees.

Consultation of a hematologist: a Dramatic decline in peripheral blood leukocytes.

Liver biopsy was not performed because of the risk of bleeding. Determine the constant blood clotting, elasticity and the formation of the clot, the content of ceruloplasmin. The level of ceruloplasmin is reduced by 53%, making 63,45 mg/l (norm 135,0±3.2 mg/l), total constant coagulation increased by 39%, compared to the norm, amounting to 32.5 min (norm 23,38±1,43 min), the elasticity of clot reduced by 31% compared to the norm, making 71,2 norm 103,2± 6,22), the formation of a blood clot increased by 77% compared to the norm, making 7,66 min (norm 4,34±0,3 min), which allows us to set the diagnosis of decompensated cirrhosis of the liver, with Reye dystrophy. Hepatic failure class With child-Pugh. Varicose veins of the esophagus 4 tbsp. Splenomegaly with symptoms of hypersplenism. Pancytopenia.

Conclusion: Given the condition expressed pancytopenia, inability to hold pathogenetic therapy for the underlying disease, the patient is recommended liver transplantation.

Example 2

Patient IV-b, 26 years old, was admitted with complaints of weakness, nosebleeds, hand tremor, change in gait, memory loss, pain in the right hypochondrium, moderate hepatomegaly appeared 5 years ago. Observed violations of functions of the Central nervous system of unknown etiology.

Admission: State of moderate severity. Thinking and speech delayed, contacts difficult, monosyllabic answers. Gait disturbed. Skin icterina, a single spider veins. Auscultation of the vesicular breathing, heart sounds clean, regular rhythm. Heart rate is 78 per minute, BP-110/70 mm Hg Abdomen is soft, abeceda tenderness in the right hypochondrium. Liver 2.5 cm stands from under the edge of the costal arch. Sat is Senka increased. Ascites and edema not.

The results of the survey:

The hemoglobin of 9.9 g/DL, erythrocytes - 3,8·109leukocyte - 0,5·10 thousand, eosinophils - 2, p/I - 2, I - 59, lymphocytes - 27, monocytes - 10, platelet - 125·109, ESR - 14 mm/h, glucose - 4.1 mmol/l, bilirubin and 15.3 µmol/l, cholesterol - 1.9 mmol/l, alkaline phosphatase - 213 IU/l, γ-GTR - 42 IU/l, AST - 40 IU/l, Alt - 24 IU/l, total protein - 74 g/l, albumin - 35 g/l, prothrombin 61%, amylase - 158 IU/l, lipase - 133 IU/l, urea - 4,9 mmol/l, creatinine - 63,7 µmol/l, iron and 15.1 mg %.

Determination of ceruloplasmin carried out by adding a solution of paraphenylenediamine, inquira for 15 min at 37°C, add a solution of sodium azide, mixing cool.

Urinalysis: UD. weight - 1010, protein - no sugar - no, leukocytes - a single in sight, erythrocytes - 2-3 in the field of view.

The content of IgA - 2.3 g/l, IgG and 19.4 g/l, IgM - 3.4 g/l, PCR HBV, HCV, HGV, HTTV neg.

The concentration of cytokines: ifγ - 240 PG/ml, IL-12 - 780 PG/ml, TNF-α - 650 PG/ml

Consultation: Identified the ring of Kayser-Fleischer.

Ultrasound: Liver - structure parenchyma is homogeneous, echo uniformly increased vascular drawing is saved. The portal vein is not extended (12 mm). Intrahepatic bile ducts are not dilated. Gall bladder: Sizes 6×2.7 cm, thickened gallbladder wall, its echogenicity is avisena; Spleen 19×11 see the Splenic vein extended (14 mm) with collaterals.

Conclusion: Signs of diffuse liver disease. Portal hypertension. Marked splenomegaly.

EGD: hyperemic Mucosa of the esophagus, there are separate varicose expanded veins of an esophagus. The fiberscope cautiously held in the stomach and duodenum. The mucosa of the stomach hyperemic, erosive changes it is not revealed. During insertion of the fiberscope, there is a small contact bleeding of the mucosa of the terminal esophagus.

Conclusion: Varicose veins of the esophagus 2-3 degrees.

Liver biopsy: a Coarse-droplet fatty degeneration, fibrosis of the liver.

Determine the constant blood clotting, elasticity and the formation of the clot, the content of ceruloplasmin. The level of ceruloplasmin is reduced by 50%67,5 mg/l; total constant coagulation increased by 35% compared with the norm, is 31,56 min; elastic clot reduced by 25% compared with the norm, is 77.4; the formation of a blood clot is increased by 75% in comparison with the norm, is 7.6 min, which allows you to diagnose cirrhosis of the liver.

The diagnosis of Reye dystrophy with primary liver disease and brain structures. Cirrhosis of the liver active, asthma. Pécs is the night the failure of class C according To child-Pugh. Varicose veins of the esophagus 4 tbsp. Splenomegaly with symptoms of hypersplenism. Pancytopenia.

Conclusion: Given the condition expressed pancytopenia, inability to hold pathogenetic therapy for the underlying disease, the patient is recommended liver transplantation.

Example 3

The patient in 38 years, when receiving complains of weakness and pain in the right hypochondrium.

Objectively admission: General condition is satisfactory. The skin is a normal color, sclera subcarina. Auscultation of the vesicular breathing, wheezing no. Heart sounds clean, regular rhythm. Blood pressure and heart rate is within normal limits. The abdomen is soft, painful to palpation in the right hypochondrium. The liver is 1 cm from the edge of the costal arch. The spleen is enlarged.

Urinalysis: No abnormalities. The level of immunoglobulins in the blood serum IgA - 1.6 g/l, IgG, and 13.2 g/l, IgM 1.1 g/l; PCR HBV, GCS, HGV, HTTV - negative.

The concentration of cytokines FL-γ - 180 PG/ml, IL-12 - 550 PG/ml, TNF-α - 620 PG/ml

Hemoglobin, erythrocytes, leukocytes reduced; WBC - within normal limits; thrombocytopenia, prothrombin decreased, glucose is within the normal range;

Determine the constant blood clotting, elasticity and the formation of the clot, the content of ceruloplasmin. While reducing Torulopsis the ina by 49% that was 68,85 mg/l; the increase in the overall constant coagulation 34% compared to the norm that was 31,33 min; reducing the elasticity of the bunch at 24% compared to the norm that was 78,43; increasing the time of formation of a blood clot by 74% compared with a rate that was at 7.55 min, judged on the presence of chronic hepatitis with Reye dystrophy.

Determination of ceruloplasmin carried out by adding a solution of paraphenylenediamine, inquira for 15 min at 37°C, add a solution of sodium azide, mixing cool.

Consultation: Identified the ring of Kayser-Fleischer.

Ultrasound: Liver - structure parenchyma is homogeneous, echo uniformly increased vascular drawing is saved. The portal vein is not extended (11 mm). Intrahepatic bile ducts are not dilated.

The spleen is slightly enlarged. The splenic vein is expanded.

Conclusion: Signs of diffuse liver disease. Portal hypertension. Splenomegaly.

EGD: On the mucosal surface of the esophagus indicates the presence of varicose veins. The mucosa is hyperemic, erosive changes it is not revealed.

Conclusion: Varicose veins of the esophagus.

Liver biopsy: Globular fatty degeneration, fibrosis.

The diagnosis of Reye dystrophy with chronice is Kim hepatitis. Varicose veins of the esophagus 1-2 degrees. Splenomegaly.

Conclusion: Given the condition of the patient is assigned pathogenetic therapy kuprenilom.

Example 4

Patient Z., 37 years old, complains of dull pain in the right upper quadrant, fever after hypothermia. When entering there is hepatomegaly, moderate Splenomegaly, subikterichnost sclera. The skin is a normal color. Heart sounds clean, regular rhythm. Blood pressure and heart rate is within normal limits. The abdomen is soft, painful to palpation in the right hypochondrium. The liver performs at 1.5 cm from the edge of the costal arch. The spleen is slightly enlarged. Ascites and edema not.

The results of the survey:

Urinalysis - without a pathology, the level of immunoglobulins in the blood serum IgA, IgG, IgM - above normal, the levels of cytokines (IFSγ, IL-12, TNF-α) - above the norm.

Hemoglobin, erythrocytes, leukocytes reduced; WBC - within normal limits; thrombocytopenia, prothrombin decreased, glucose is within normal limits.

Determine the constant blood clotting, elasticity and the formation of the clot, the content of ceruloplasmin. Note the decrease in the content of ceruloplasmin by 43% compared to the norm that was 76,95 mg/l; the increase in the overall constant blood coagulation by 18% compared to the norm that was 27.6 min; reduced elastic cuff, full the particular clot by 12% in comparison with the norm, that was 90,82; increasing the time of formation of a blood clot by 21% compared to the norm, which amounted to a 5.25 minutes These changes allow the diagnosis: chronic hepatitis, Reye dystrophy.

Determination of ceruloplasmin carried out by adding a solution of paraphenylenediamine, inquira for 15 min at 37°C, add a solution of sodium azide, stirring, cool.

Consultation: Identified the ring of Kayser-Fleischer.

Ultrasound: Liver - structure parenchyma is homogeneous, echo uniformly increased vascular drawing is saved. The portal vein is not expanded. Intrahepatic bile ducts are not dilated. Gall bladder: Dimensions slightly increased, the echo wall is increased. The splenic vein is expanded.

Conclusion: Signs of diffuse liver disease. Portal hypertension. Splenomegaly.

EGD: On the mucosal surface of the esophagus indicates the presence of varicose veins. The mucosa is hyperemic, erosive changes it is not revealed.

Conclusion: Varicose veins of the esophagus.

Liver biopsy: naznachen marked portal fibrosis; in one of the slices, a sinusoid, a small group of macrophages, isolated areas of proliferation Kupperbusch cells; a significant number of grains of brown pigment in ciepla the IU hepatocytes, located mostly in the Central parts of the slices.

The diagnosis of Reye dystrophy with primary liver disease. Chronic hepatitis. Splenomegaly.

The patient is assigned pathogenetic treatment kuprenilom.

According to the invention a differential diagnosis of chronic hepatitis and cirrhosis of the liver, with Reye dystrophy diagnosed in 21 patients. The results of treatment and follow-up observations confirmed the accuracy of differential diagnosis on the basis of the proposed quantitative criteria.

Sources of information

1.Bearn A.G. Wilson disease. Am J. Med., 1957, 22, 747.

2. SU 1772754 A1, publ. 30.10.1992.

The method of differential diagnosis of chronic hepatitis and cirrhosis of the liver, with Reye dystrophy by biochemical analysis of blood serum, wherein determine the constant blood clotting, elasticity and the formation of the clot, the content of ceruloplasmin and by decreasing the level of ceruloplasmin on 43-49% and the increase in the overall constant coagulation 18-34% compared with normal and decreased elasticity of the bunch for 12-24% in comparison with the norm, increasing the time of formation of a blood clot on 21-74% compared to the norm is judged on the presence of chronic hepatitis with Reye dystrophy and p and reducing the level of ceruloplasmin by 50% or more and the increase in the overall constant coagulation by 35% and more in comparison with normal and decreased elasticity of the bunch of 25% or more compared to the norm, increase the time of formation of a blood clot on 75% or more compared to the norm is judged on the presence of cirrhosis with Reye dystrophy.



 

Same patents:

FIELD: medicine, ophthalmology.

SUBSTANCE: in patients with rheumatoid uveitis in acute period of disease, that is within the 7th to the 10th d one should detect the concentration of tumor necrosis factor (TNF-α) concentration in blood serum. At its content being 150 pcg/ml and higher the flow of rheumatoid uveitis is considered to be favorable, and at concentration ranged 50-100 pcg/ml - unfavorable. The innovation increases the accuracy in predicting the severity degree of the flow of rheumatoid arthritis and provides the chance for conducting adequate medicinal therapy.

EFFECT: higher efficiency of therapy.

6 ex

FIELD: medicine, juvenile endocrinology.

SUBSTANCE: one should detect the level of endothelin-1 (pcg/ml) and basic fibroblast growth factor (bFGF) (ng/ml) in patients with insulin-dependent diabetes mellitus in blood serum. At the ratio of endothelin-1 against bFGF being equal to 40 and higher it is possible to diagnose subclinical stage of diabetic neuropathy (DNP) in children. The innovation provides the opportunity for prescribing adequate therapy in due time and, thus, prevent the development of severe complications.

EFFECT: higher accuracy and objectivity.

2 ex

FIELD: medicine, neonatology.

SUBSTANCE: it is necessary to determine neopterin concentration in spinal fluid and in blood serum. At values of neopterin concentration being 51.6±7.29 nM/l in blood serum and 16.3±3.80 nM/l in spinal fluid one should diagnose intracranial hemorrhage, moreover, one should apply the technique of immunoenzymatic assay that requires minimal quantity of biological liquids. The innovation is simple in implementation, requires small quantities of biological liquids that enables to apply it widely in resuscitation departments, neonatal pathology departments and in maternity hospitals.

EFFECT: higher accuracy of diagnostics.

2 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: method involves determining transforming growth factor in male saliva β1 (TGF-β1) using immunoenzyme assay method with a set of standards for determining the TGF-β1. The TGF-β1 value growing 2, 4 and more times as large when compared to a value taken for healthy patient norm, bronchus remodeling is to be diagnosed. The value taken as healthy patient norm is equal to 403.88±102.58 selected as a result of forced expiration volume per 1 s comparison in healthy patients and bronchus biopsy results.

EFFECT: high information capacity, safety and reliability of diagnosis method.

2 cl, 2 tbl

FIELD: medicine.

SUBSTANCE: method involves determining lactate dehydrogenase activity and NADP-dependent glutamate dehydrogenase. Lactate dehydrogenase activity being equal to or greater than 12.50 mcU and NADP-dependent glutamate dehydrogenase activity being equal to or less than 0.25 mcU, no metastases in lung root lymph node in pulmonary carcinoma cases are thought to be available. Lactate dehydrogenase activity being less than 12.50 mcU and NADP-dependent glutamate dehydrogenase activity being greater than 0.25 mcU, the metastases are proved to be available.

EFFECT: high accuracy of diagnosis.

FIELD: veterinary medicine.

SUBSTANCE: method involves determining protein expression level induced during pregnancy in an animal with desirable pregnancy status. Protein expression level of this animal is compared to the protein expression level in non-pregnant animals.

EFFECT: high accuracy and reproducibility of pregnancy test.

36 cl, 4 dwg

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: at detecting tumoral ovarian neoplasm, at the stage of preoperational procedure one should additionally detect the concentration of alpha-2-glycoprotein (ABG) in blood serum due to rocket immunoelectrophoresis, and at its concentration being 0.008-0.019 g/l one should diagnose benign ovarian tumoral process and match laparoscopic access in case of operative interference; at concentration of 0.024 g/l and more one should diagnose high risk for availability of malignant tumoral process and match laparotomy as an access for operative interference. Thus, the innovation provides the reason for matching the access for operative interference that enables to decrease the frequency of traumatic interference and risk of postoperational complications.

EFFECT: higher efficiency.

4 ex, 1 tbl

FIELD: medicine, cytology.

SUBSTANCE: one should sample cell material from affected part due to puncture biopsy or, at availability of free excreta out of teats, with exfoliative technique and cytological testing of microscopy-obtained material. Decision on the fact of availability or absence of mammary tissue pathology should be established according to the presence or absence proliferative cells, cells with foamy cytoplasm, and cell-free protein masses. Moreover, if proliferative cells are absent, cells with foamy cytoplasm are, also, absent or their quantity is low and one can observe cell-free protein masses it is possible to diagnose the absence of pathology. If proliferative cells are present, and cells with foamy cytoplasm and cell-free protein masses are absent the state should be evaluated as pathological; if proliferative cells, cells with foamy cytoplasm and cell-free protein masses are present the state should be evaluated as instable and control-demanding. The innovation enables to detect optimal medicinal tactics and conduct control for therapeutic efficiency.

EFFECT: higher objectivity of investigation.

1 cl, 1 dwg, 3 ex, 1 tbl

FIELD: pulmonology and thoracic medicine.

SUBSTANCE: method is characterized by that on first or second day from the beginning of treatment blood serum of patient is analyzed on the level of peptide-bound hydroxyproline. When this level is increased to 82.44 μmol/L and higher. Development of acute metapneumonic nonspecific pleural empyema is prognosed.

EFFECT: use of the method allows performing early prognosis of acute metapneumonic nonspecific pleural empyema and preventing this sickness.

3 ex

FIELD: medicine, emergency surgery.

SUBSTANCE: the present innovation could be applied in clinical practice for evaluating the severity degree in patients with perforating gastric and duodenal ulcer due to the following technique: in patient's blood at the moment of entrance, before operation one should detect the content of catalase. At its level being 3620-2300 U/g/min it is necessary to evaluate patient's state to be severe. The innovation favors to prescribe the complex of curative procedures in due time.

EFFECT: higher accuracy of detection.

6 ex

FIELD: medicine, mycology, infectious diseases, hematology, pulmonology, dermatology, therapy, neurology, allergology.

SUBSTANCE: the present innovation deals with blood sampling (0.02 ml) to put the sample into physiological solution (4 ml) stained with gentian violet at 10 mg/100 ml physiological solution. After a 5-min-long staining a tube's content should be mixed with a glass stick and put onto Goryaev's counting chamber to detect stained mycotic spherules and fulfill their quantitative detection in 100 large squares. The innovation suggested provides quick diagnostics of pecilomycosis.

EFFECT: higher accuracy and rate of diagnostics.

7 dwg, 2 ex, 2 tbl

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: it is necessary to study cellular residue of 1 ml liquor, calculate the quantity of cells with the signs of apoptosis and at their quantity being about 9-10/1 sq. mm one should predict lethal result in children with perinatal lesion of central nervous system.

EFFECT: higher accuracy of prediction.

2 ex

FIELD: medicine.

SUBSTANCE: method involves studying quantitative indices of peripheral blood and other clinical anamnesis disease signs like intoxication syndrome, cutaneous itch, hyperkeratosis palmaris et plantaris, hair thinning, polylymphoadenopathy and white dermographism. The indices are calculated by applying linear discriminant analysis method producing formulas for calculating variables usable for determining various erythrodermatitis cases (F1 and F2; F3, F4, F5). The values are compared to each other and one or other disease type is diagnosed.

EFFECT: high accuracy of diagnosis; shortened prediagnostic period.

6 dwg, 5 tbl

FIELD: medicine.

SUBSTANCE: the present innovation deals with affecting blood with the suggested preparations: under laboratory conditions one should apply 1 ml patient's blood stabilized with 3.8%-sodium citrate solution at the ratio of 1:9 to be introduced by drops at the quantity of 0.1-0.2 ml and mixed. A timing device should be applied and in case of blood clots for 1 min, not longer it is possible to conclude upon the presence of hemostatic effect. The innovation enables to shorten the period for investigation at increased significance of the results obtained.

EFFECT: higher accuracy of evaluation.

3 ex

FIELD: medicine.

SUBSTANCE: method involves determining secretory and serum immunoglobulin IgA. The first value being less than 1.42±0.07 g/l and the second one less than 1.20±0.05 g/l, complications are predicted to occur as multiple caries and periodontium tissue inflammation after applying bracket system treatment.

EFFECT: high accuracy of prognosis; prevented complications.

3 tbl

FIELD: veterinary science.

SUBSTANCE: in blood it is necessary to determine the ratio of lymphocytes against segmented neutrophils and at its values being up to 5.0 one should consider the resistance to be high, at 5.0-9.9 - as decreased, and from 10.0 and higher - as low. The innovation enables to evaluate physiological state in animals quickly and accurately.

EFFECT: higher efficiency and accuracy of evaluation.

4 ex, 2 tbl

FIELD: medicine, psychiatry, neurology.

SUBSTANCE: one should detect the degree of depression, the quantity of monocytes and lymphocytes in leukocytic formula of total clinic blood analysis, and, also, the values of infra-red spectroscopy of the whole capillary blood. At the values of average absorption being 35.1±2.2%, 36.3±1.7% and 36.9±1.3% in the ranges of 3085-2832cm-1, 1543-1425 cm-1 and 1127-1057 cm-1, correspondingly, and normal content of lymphocytes and monocytes it is possible to diagnose depression, and at the values of average absorption being 43.5±1.8%, 47.9±3.2% and 45.2±1.5% in the ranges of 3085-2832cm-1, 1543-1425 cm-1 and 1127-1057 cm-1, correspondingly, in combination with monocytosis being above 5% and lymphocytosis being above 25% one should diagnose therapeutically resistant depression in case of endogenous psychic diseases. Application of the present innovation enables to detect persons with immunological and pathobiochemical signs of affected activity of nervous system being characteristic for therapeutically resistant depression that, in its turn, provides the opportunity to form adequate therapeutic strategy in due time.

EFFECT: higher efficiency and accuracy of diagnostics.

2 ex, 1 tbl

FIELD: medicine, psychiatry, neurology.

SUBSTANCE: in patients with endogenous psychic disorders one should detect the degree of depression by Hamilton's scale, the quantity of monocytes and lymphocytes in leukocytic formula of total clinic blood analysis, and, also, the values of infra-red blood spectroscopy in infra-red spectroanalyzer. At degree of depression being 20.9±2.2 points, the values of average absorption being 43.5±1.8%, 47.9±3.2% and 45.2±1.5% in the ranges of 3085-2832 cm-1, 1543-1425 cm-1 and 1127-1057 cm-1, correspondingly, in combination with immunity values as monocytosis being above 5% and lymphocytosis being above 25% in leukocytic formula of total blood analysis one should diagnose therapeutically resistant depression at endogenous psychic diseases. Application of the present method enables to detect persons with immunological and pathobiochemical signs of affected activity of nervous system being characteristic for therapeutically resistant depression that, in its turn, provides the opportunity to form adequate therapeutic strategy in due time.

EFFECT: higher accuracy and efficiency of diagnostics.

1 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: method involves examining biological substrate, blood serum taken from patient, the latter being preliminarily dried and dissolved in Vaseline oil. Infrared spectroscopy examination of sample is carried out with absorption spectra being recorded in the bandwidth of 1200-1000 cm-1 and absorption band peak heights are recorded with maximum occurred at 1170, 1165, 1150, 1140, 1130, 1100, 1070 and 1025 cm-1. Ratios of each preceding to the following peak value are calculated as 1170/1165, 1165/1150, 1150/1140, 1140/1130, 1130/1100, 1100/1070, 1070/1025 and the S value is determined as ratio of peak having maximum at 1100 cm-1 to the mean pf all particular values. S being greater than 0.66, severe acute pancreatitis clinical course is predicted.

EFFECT: high accuracy of diagnosis; accelerated examination procedure.

FIELD: medicine, gynecology.

SUBSTANCE: the diagnostics should be fulfilled due to inoculating the urine under testing onto the surface of different media followed by selecting bacterial colonies to detect their capacity to destruct colicin V. After calculation one should detect the severity degree of the disease mentioned. The present innovation enables to diagnose both light and severe degrees of the disease in question.

EFFECT: higher accuracy of diagnostics.

3 ex

FIELD: medicine.

SUBSTANCE: invention relates to laboratory methods for blood analysis. Plasma is dropped in copper sulfate solution with density 1.023 g/cm3, not above, and time for drop falling on bottom of graduated cylinder with column height 243 mm is measured. The blood plasma density value is calculated by the formula:

wherein is the unknown blood plasma density (g/cm3); is copper sulfate solution density measured by areometer (g/cm3); t is average falling time of plasma drop in the copper sulfate solution (as seconds); 0.260130126 and 0.00290695 are correction coefficients. Temperature of plasma and copper sulfate solution is 20oC. Method is simple and suitable and allows carrying out analysis of small volumes of blood plasma and to reduce analysis time.

EFFECT: improved assay method.

2 ex

Up!