The method of obtaining medicinal healing sponges |
|
|
IPC classes for russian patent The method of obtaining medicinal healing sponges (RU 2104008):
 Absorbent polymer composition / 2091081
The invention relates to an improved particulate, absorbent, polymeric compositions
 Composition for microporous material krasavicaaaaa / 2057150
The invention relates to the production of PVC plastisols with coloured compounds, which serve as the basis for the manufacture of krasavitsa materials in the form of various rollers of printing machines, stamp pad, marking stamps, etc
The way to prevent cancer / 2099080
The invention relates to the field of biology, in particular to the section of medicine-Oncology
 A method of treating breast cancer and cancer of the womb / 2093176
The invention relates to oncoimmunology and can be used for adjuvant immunotherapy of cancer patients and, in particular, patients with breast cancer and cancer of the womb
 A method for the treatment of exacerbated chronic periodontitis / 2092182
The invention relates to medicine, namely to dentistry
The method of obtaining hyaluronidase / 2090207
The invention relates to the production of enzyme preparations used in medical practice, namely the method of production of hyaluronidase, which is the active ingredient of the drug "Lydasum and Ronidase", used in the treatment of scars, adhesions, contractures and stiffness of custalow, nonhealing wounds
Composition, penetrating through the skin, for the treatment of osteoarthritis / 2088239
The invention relates to medicine, namely to traumatology, Orthopaedics and rheumatology, and is intended for treatment of early-stage osteoarthritis deformans
Wound-healing coating / 2085217
The invention relates to medicine and can be used in surgery as a dressing for treating wounds, burns, ulcers; in ophthalmology as ocular drug films; in dentistry in the surgical treatment of parodontosis
The method of treatment of peptic ulcer of the duodenum / 2082413
The invention relates to medicine, namely to gastroenterologie, and can be used for the treatment of duodenal ulcers
 A method of treatment of endometritis / 2072866
The invention relates to medicine, specifically to obstetrics and gynecology, and for the treatment of endometritis
For the treatment and prevention of dysbacteriosis / 2071339
The invention relates to medicine and can be used in Pediatrics, gastroenterology, obstetrics and gynecology, as well as in veterinary medicine
 Means for stimulation of the motor-evacuation function of the intestine in children / 2070050
The invention relates to medicine, namely to manufacture products for medical nutrition, and can be used in cases involving constipation and disorders of the bile passage in children
 Medicinal composition for the treatment of herpetic keratitis and keratoconjunctivitis / 2104002
The invention relates to medicine, specifically to medicines for treatment of herpes infections of the eye
The way preoperative preparation of the patient / 2099060
The invention relates to medicine and can be used in anesthesiology, General surgery and neurosurgery
 The drug is affecting the functional activity of eukaryotic cells and prokaryotes / 2098114
The invention relates to biochemistry
 Irrigation solution for ophthalmic operations / 2098100
The invention relates to medicine, namely to ophthalmology, and can be used to flush lenticular masses during cataract extraction, filling of chamber space for various surgical interventions
 A method of treating bone-cerebral form of acute radiation sickness / 2097059
The invention relates to medicine and can be used in radiobiological experimental studies in mammals to study the mechanism of action of radiation on the body and in practical health care for the treatment of bone marrow form of acute radiation sickness
 Antihypoxic agent and method thereof / 2096043
 The method of receiving parenteral applied renal diagnostic tools / 2095076
The invention relates to medicine, namely to a method for parenterally applied renal diagnostic products containing fructan
 The method of producing dextran / 2093577
The invention relates to the field of biotechnology and chemical technology, particularly to a method of depolymerization of dextrans, namely the method of production of dextran from srednevekovoi molecular weight (m m) 1000 D, which can be used in the medical industry, the drug is able to prevent severe anaphylactic reactions during transfusion dextranomer products with higher m m
|
(57) Abstract: The invention relates to the pharmaceutical industry. The method includes preparation of solutions of polysaccharides and bioactive substances, consistent blending components, freeze-drying the mixture. As polysaccharides use of alginic acid and its salts, a mixture of salts of alginic acid with chitosan, carrageenan; as a biological active substances used hyaluronidase, proteolytic complex, cytochrome C and deoxyribonucleic acid (DNA) or biochemically compatible mixture. 1 g of the final product must contain 5-15 cytochrome C, from 15 to 50 conventional units of proteolytic activity, 30-60 conventional units hyaluronidase activity, 80-120 mg DNA, and in the case of mixtures - the number corresponding to the lower value of the interval of concentrations. 3 C.p. f-crystals. The invention relates to pharmaceutical industry and can be used for the manufacture of drugs intended for the treatment of purulent-necrotic wounds, burns, trophic ulcers, scars, bruises, etc. A method of obtaining porous material with wound-healing action and calcium lactate, and furatsilinom, freeze-drying the mixture to obtain drug sponges, having good mechanical properties. The closest technical solution is the method of obtaining porous material based on alginic acid containing sterility (A. L. Komissarov, V. S. Yakubovich, P. I. Tolstykh, I. F. skokova, A. D. Vernick. Obtaining a porous material based on alginic acid containing immobilized terrilian // Antibiotics and chemotherapy, 1988, T. 733, No. 10, S. 735-739). The method consists in the immobilization by ionic accession proteolytic enzyme terrilian on alginic acid to obtain a porous medical sponge with a ratio of enzyme : native 1:4 to 1:20. Solutions of polysaccharide and enzyme are mixed and the mixture is dried by sublimation method. The drug speeds up the cleansing of the wound surface from necrotic masses, reduces microbial colonization of wounds. The disadvantage of this method is the limited specificity of therapeutic action. The purpose of the invention is the expansion of the range and specific therapeutic action drug vulnerary sponges based on polysaccharides. The essence of the proposed method is freeze-drying, thus the solution of sodium alginate or a solution of carrageenate mixed with hyaluronidase or proteolytic complex or deoxyribonucleic acid (DNA in such concentration to 1 g of the final product consisted of 30-60 conventional units hyaluronidase activity or 15-50 conventional units of proteolytic activity, or 80-120 mg DNA. With the introduction of the sodium alginate or carrageenan mixture of proteolytic complex and DNA, their number per 1 g of the final product must match the lower value of the interval of the individual concentrations. When receiving drug sponges based alginate calcium-sodium in a solution of sodium alginate enter 15-50 conventional units of proteolytic activity or 5-25 mg of cytochrome C per 1 g of the final product, and then with vigorous stirring, a suspension of calcium gluconate, the ratio of sodium alginate and calcium alginate is 1:1 to 1:5. When receiving drug sponges on the basis of a mixture of polysaccharides of sodium alginate and chitosan, taken in quantities of 1:0.5 to 1:1, enter 5-25 mg of cytochrome C or 80-120 mg DNA, or their mixture in amounts corresponding to the lower value of the interval of concentrations. Chrome is osla - of sodium alginate from kelp Japanese or carrageenan from Chondrus armatus. For this purpose, a portion of dry algae pour water in the ratio 1:10 add formalin to 0.5%, withstand 10-12 hours the Solution is poured and poured swollen algae with 0.5% solution of hydrochloric acid in the ratio of 1:15, after 2 h, the solution is drained, the seaweed is washed with water to achieve a pH in the wash water 7. Then algae pour hot water in the ratio 1:40 add sodium carbonate at the rate of 10% of the original sample of dry algae, heat the mixture up to 85-90oC and incubated for 4-8 hours until complete dissolution of algae. The resulting mixture is filtered, the filtrate is cooled and used for immobilization of biologically active substances. Example 1. To 24 g of sodium alginate was added to 3400 ml of distilled water, left to dissolve for 12 h, and then brought the solution to 4700 ml, boiled for 10 min, cooled and under vigorous stirring was added 100 ml of hyaluronidase solution containing 950 units of activity. After complete dissolution of the enzyme mixture is poured into the cuvette, was frozen at minus 40oC and sublimated at a temperature of finished product not more than 30oC. The resulting material is a porous plastino and bruises. Example 2. To 40 g of alginate was added to 4000 ml of water, kept for 12 h, then the solution is brought up to 7600 ml, boiled for 10 min, cooled. 4.0 g of DNA was combined with 200 ml of water, stirred until smooth, brought to the boil and cooled. 1.2 g of proteolytic complex activity of 750 units was dissolved in 200 ml of water. Then with vigorous stirring connected solutions of sodium alginate, proteolytic complex and DNA. The mixture was poured into cuvettes were frozen and sublimated, as in example 2. The resulting material is a porous plate, light brown, soluble in water, accelerates healing of purulent actoricesti, viagrawwirkung wounds. Example 3. To 50 g of sodium alginate was added to 5000 ml of water, was left to dissolve for 12 h, boiled for 10 min, cooled, 12.5 g of calcium gluconate mixed with 4500 ml of water until a homogeneous suspension. 4 g of proteolytic complex, containing 2000 units of activity, was dissolved in 500 ml of water. To a solution of sodium alginate with vigorous stirring was added first a solution of proteolytic complex, and then a suspension of calcium gluconate. The mixture was poured into the cell, stood 20 min for gel formation, Zam is watoga color, poorly soluble in water, accelerate the healing of infected wounds with purulent-necrotic content. Example 4. 20 g of dry alginic acid was poured 3000 ml of water, stirred and added 6 g of sodium carbonate maintained until complete dissolution of the components, the solution was boiled for 10 min, cooled. Preparing a suspension of 6 g of calcium gluconate, stirred until smooth in 900 ml of water, 150 mg of cytochrome C was dissolved in 100 ml of water. To a solution of sodium alginate solution was added cytochrome C, were thoroughly mixed and added to a suspension of calcium gluconate. The mixture was poured in the cell was left for 20 min, frozen and dried as in example 1, the resulting material is a rigid porous plate pale pink color, slbo soluble in water, are recommended for the treatment of burns 1 degree. Example 5. 34 g of dry kelp was filled with 1000 ml of water were added 5 g of formalin, kept for 10 hours Then the solution was decanted, swollen algae filled in 1100 ml of 0.5% hydrochloric acid was stirred for 2 h with periodic stirring, the acid solution was decanted, the algae were washed with water until the pH of wash water 7. The washed seaweed was filled with 3000 ml of hot water, was added 3,4 sodium carbonate, heated cooled. 1 g of DNA was dissolved in 100 ml of water under heating and thorough mixing of the combined solutions were frozen and dried as in example 1. The resulting material is a porous plate, light brown, accelerates granulation volosataya wounds. Example 6. 16.6 g of dry algae was filled in 100 ml of water, then carried out the operation as in example 5. Under stirring with a solution of BAS to the filtrate, containing carrageenan was added 100 ml of hyaluronidase solution containing 180 units of activity. After mixing, the mixture was frozen and sublimated, as in example 1. The resulting material is a porous soft cream color plates, accelerates resorption of scars and bruises. Example 7. 6 g of dry sodium alginate was filled in 100 ml of water, left for 1 h, boiled for 10 min, cooled. 6 g of chitosan was dissolved in 600 ml of 0.3% acetic acid and 1.15 g of DNA was dissolved in 300 ml of water by heating, 80 mg of cytochrome was dissolved in 100 ml of water. To a solution of sodium alginate was added first, cytochrome C, DNA and then the last chitosan, frozen and dried as in example 1. The resulting material is an elastic porous plate, poorly soluble in the wound sponges based on polysaccharides, including preparation of solutions of polysaccharides and bioactive substances, their mixing and freeze-drying, characterized in that for the preparation of polysaccharide solutions using alginic acid and its salts, a mixture of salts of alginic acid with chitosan, carrageenan, and as biologically active substances using hyaluronidase, cytochrome C, proteolytic complex and deoxyribonucleic acid or biochemically compatible salt. 2. The method according to p. 1, characterized in that the sodium alginate and carrageenan injected hyaluronidase, or proteolytic complex, and/or deoxyribonucleic acid, alginate calcium injected cytochrome C or proteolytic complex, and a mixture of sodium alginate with chitosan injected cytochrome C and/or deoxyribonucleic acid, 1 g of the final product is administered 5 to 25 mg of cytochrome C, 15 to 50.E. proteolytic activity of 30 to 60.E. youroriginal activity, 80 to 120 mg deoxyribonucleic acid. 3. The method according to PP.1 and 2, characterized in that in the preparation of polysaccharide solutions of sodium alginate and chitosan take in the ratio of 1 0,5 1 1, and the sodium alginate and calcium alginate in a ratio of 1 1 of 1 5. 4. Cocoaheads thus, to their number per 1 g of the final product corresponded to the lower value of the interval of concentrations.
|
