Method for neurohormonal correction and rejuvenation using musical-acoustical exposure |
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
IPC classes for russian patent Method for neurohormonal correction and rejuvenation using musical-acoustical exposure (RU 2518538):
Method for assessing dermal exposition of pesticides in workers / 2518361
Method involves preparing a series of exposed lavages of worker's various skin areas in the natural processing environment with preparations using a recommended washing liquid, conducting measuring identification of a substance by chemical analysis, calculating average dermal exposition (Dav); as a biological model for preparing the exposed lavage is prepared an isolated pig skin; that is followed by applying the measured amount of the desired substance and lavages for at least 10 times; the average lavage completeness of the applied amount (K, %) that is included into the average dermal exposition (Dav) formula.
Method for determining stage of iron deficiency / 2518316
Invention refers to medicine, particularly to obstetrics, paediatrics, therapy and may be used to determine a stage of iron deficiency both in pregnant women and children, and other social groups. The method consists in the fact that serum iron and ferritin are determined at the same time, and if serum iron is less than 12.0 mcmole/l, and ferritin is 15 mcg/l or more, the first stage of iron deficiency is stated; if serum iron is 12.0 mcmole/l or more, and ferritin is less than 15 mcg/l, the second stage of iron deficiency is stated; serum iron less than 12.0 mcmole/l and ferritin less than 15 mcg/l show the third stage of iron deficiency.
 Method and system for determining quality of cultivated cells / 2517618
Claimed invention relates to the field of microbiology. Group of inventions includes a method and a system for determining quantity of cultivated microbial cells, such as E. coli, Ps.aeroginosa, or a mixture of microbial cells (for instance, for determination of the total number of microbes) in an analysed sample. The said method includes (i) bringing the analysed sample, which can include cultivated microbial cells in contact with, at least, one signal agent, capable of binding with the microbial cell membrane, with the said bringing in contact being carried out for the preliminarily specified first time period (T1), sufficient for integration of the signal agent into the microbial cell to such a level at which the signal, emitted by the sample, in fact reaches a plateau; (ii) removal of non-internalised signal agent from the said analysed sample; (iii) identification of the signal cultivated cells among the signal objects in the said sample, based on selection parameters, preliminarily determined for the said cultivated cells, during the second time period (T2), following the said first time period (T1), during which the said signal in fact remains at the level of the said plateau; and (iv) determination of a quantitative value, indicating the number of the cultivated cells in the analysed sample on the basis of the said selected objects.
Method for prediction of thrombus flotation in inferior vena cava with using molecular genetic monitoring additionally / 2517215
Invention refers to medicine and aims at prediction of floating deep venous thrombosis in the inferior vena cava. Clinical laboratory and instrumental methods of examination are applied, and if observing the signs related to deep venous thrombosis, molecular genetic analysis of the first-type plasminogen activator inhibitor (PAI-1) gene is performed additionally. If a polymorphous variant 675 4G/4G of the PAI-1 gene, the presence of the thrombus flotation is predicted.
 Method of determining ligand content in sample (versions) / 2517161
Claimed invention relates to biology and medicine, namely to the field of biological-chemical analysis, and makes it possible to define qualitative or quantitative presence of a ligand in a sample. Essence of invention consists in a method of determining content of molecules of, at least, one type of the ligand in the sample, in which selected is either an agent or a carrier, which has a blocked object, unable to specifically interact with the ligand to be identified and which takes part directly or indirectly in production of a detectable signal, and change of the detectable signal, by which the ligand in the sample is identified is influenced either by space or space-electrostatic blocking of the said blocked element.
Method of predicting efficiency of immunotherapy basing on estimation of level of expression of cytokine mrnk in kidney cancer tissue / 2517082
Invention relates to the field of medicine. Claimed is a method of determining sensitivity of kidney cancer to immunotherapy after performing nephrectomy, including diagnosis verification, performing nephrectomy of kidney cancer, sampling of tumour and normal kidney tissue to determine a level of expression of cytokines IL-4, IL-6, IL-10, TGFβ and calculation of ratio of level of cytokine expression in tumour and normal kidney tissue. If the level of cytokine expression in tumour tissue of kidney is higher than normal, therapy is considered to be indicated.
 Differential diagnostic technique for hepatitis and hepatic cirrhosis / 2516970
Patient's blood serum is additionally examined for laminin-1 IgG antibodies. If the derived value is 0 to 11.9 Unit/ml, hepatitis is stated; the value within the range of 12 to 17.9 Unit/ml show a controversial result requiring a re-examination three month later, while the value of laminin IgG antibodies being 18 Unit/ml or more, hepatic cirrhosis is diagnosed.
 Muteins of tear lipocalin, possessing affinity to human c-met receptor tyrosine kinase and methods of obtaining them / 2515063
Invention relates to field of biotechnology, namely to muteins of human tear lipocalin, and can be used in medicine. Mutein of human tear lipocalin (hTLc) has identifiable affinity of binding with human receptor Met (c-Met) receptor tyrosine kinase, or its domain, or fragment of human c-Met. Mutein contains from 6 to 18 amino acid substitutions relative to amino acid sequence of mature lipocalin of human tear liquid (SWISSPROT DATABANK ENTRY P31025; SEQ ID NO:36), selected from group, consisting of Arg 26→Thr, Val, Pro, Ser, Gly; Glu 27→Gln, Gly, Val, Ser; Phe 28→Met, Asp; Pro 29→Leu, Ile, Ala, Trp; Glu 30→Leu, Gly, Arg, Phe; Met 31→Ser; Asn 32→Leu, Arg, Val, Gln; Leu 33→Tyr, Val, Ile, Thr, Phe; Glu 34→Val, Arg, Ala; Leu 56→Asn; Ile 57→Gln; Ser 58→Ile, Val; Asp 80→Tyr; Lys 83→Ala; Glu 104→Asp; Leu 105→Thr; His 106→Trp and Lys 108→Gly. Mutein can also additionally contain the following substitutions: Cys 61→Ser; Cys 101→Ser; Cys 153→Ser; Arg 111→Pro; Lys 114→Trp; Thr 37→Ser; Met 39→Ile, Leu; Asn 48→Ser; Lys 52→Thr, Met; Met 55→Leu; Lys 65→Arg, Leu; Ala 79→Leu, Ser; Ala 86→Thr; Ile 89→Ser, Gln, Thr, His; Thr 40→Cys; Glu 73→Cys; Arg 90→Cys; Asp 95→Cys; Lys 121→Cys; Asn 123→Cys and Glu 131→Cys.
 Method for assessing negative impact of atmospheric formaldehyde on conjugation and elimination dysfunction of glutathione system in children / 2513508
Invention refers to laboratory diagnostic techniques for a negative impact of formaldehyde on the conjugation and elimination dysfunction of the glutathione system in children living on the territory with the high content of this compound in the atmospheric air. The technique implies as follows: blood is sampled from the children to determine whole blood formaldehyde and to determine a serum activity of the following laboratory parameters: glutathione peroxidase, glutathione-S-transferase and glucoso-6-phosphate dehydrogenase. The blood formaldehyde value and the above laboratory values are correlated. If the following certain dependences are simultaneously provided: high formaldehyde - low glutathione peroxidase, glucoso-6-phosphate dehydrogenase activities and high glutathione-S-transferase activity, the negative impact of formaldehyde on the conjugation and elimination dysfunction of the glutathione system of a child's body.
Method for detecting developing neointimal hyperplasia and carotid restenosis following angioplasty in patients with progressive cerebral atherosclerosis / 2513194
Blood serum lipoprotein-associated phospholipase A2 (Lp-PLA2) biomarker is measured in a patient with progressive cerebral atherosclerosis after carotid angioplasties no more than 6 months later using immunoenzyme method; if the derived value is 360 ng/ml and more, developing neointimal hyperplasia and carotid restenosis are stated.
 Method for activation of white-cell-rich suspension growth and complex correction of blood composition in acoustic field in vitro / 2518534
Blood or its fractions are exposed to broadband oscillating acoustic signals at a temperature of 4°C to 38°C within the frequency range of 16-20000 Hz with a dominating level of noise pressure more than 45 dB, and less than 60 dB at 60-80 beats per minute, or to sequentially alternating acoustic signals with the dominating level of noise pressure ≤45 dB at <60 beats per minute and acoustic signals with the dominating level of noise pressure more than 65 dB, and less than 90 dB at >80 beats per minute.
Method for determining suitability of preserved packed red cells for transfusion / 2518344
Before transfusion, a preserved blood sample of 2 ml is mixed with an ozonised solution of 0.9% sodium chloride with the ozone concentration of 2 mg/l in the equivalent volume. The 15-minute exposition is followed by evaluating the 2,3 diphosphoglycerate concentration in the prepared packed red cell suspension. The 2,3 diphosphoglycerate concentration in the packed red cell suspension more than 6 mcmole/l testifies to the high effectiveness of planned hemotransfusion.
 Method of determining space-time distribution of proteolytic enzyme activity in heterogeneous system, device for realising thereof and method of diagnosing hemostasis system disorders by change of space-time distribution of proteolytic enzyme activity in heterogenic system / 2518247
Claimed invention relates to field of biophysics. Claimed are methods of determining space-time distribution of proteolytic enzyme activity in heterogeneous system, in accordance to which: provided is system in vitro, which contains sample of blood plasma, whole blood, water, lymph, colloidal solution, crystalloid solution or gel, and proteolytic enzyme or its precursor; fluorogenic, chromogenic or luminescent substrate for said enzyme id added; space distribution of signal of released substrate label is registered at specified time moments and space-time distribution of proteolytic enzyme activity is obtained by solving reverse problem of type "reaction-diffusion-convection" taking into account label binding with medium components. Also described is device for realisation of methods in accordance with claimed invention and method of diagnosing hemostasis disorders, based on their application.
 Diagnostic technique for malignant growth generalisation / 2517527
Technique involves preparing blood serum; preparing at least 10 serum samples by applying the serum on glass slide; drying simultaneously under a cover glass at room temperature and the relative humidity of 70-75% for 7-10 days; studying by polarised light microscopy and detecting anisotropic spherolites. If observing the anisotropic spherolites having a curved structure, the malignant growth generalisation is diagnosed. If observing the anisotropic spherolites having a granular structure or a combination of the spherolite with the included microspherolite with the granular structure, no generalisation is diagnosed.
Method for prediction of clinical outcome of sepsis / 2517523
Venous blood of a patient suffering from sepsis of abdominal origin is analysed twice every 1 to 7 days; vascular endothelial growth factor (VEGF) is evaluated in pg/ml using an enzyme-immunoassay, a prognostic index (PI) of the clinical outcome of sepsis of abdominal origin is calculated by formula. If the PI index is less than 100%, the unfavourable outcome of sepsis of abdominal origin is predicted.
Method for choosing management of pregnant women with placental insufficiency and foetal growth inhibition syndrome / 2517374
Blood is examined for angiogenic factors, namely soluble fms-like tyrosine kinase (sFlt-1) and placental growth factor (PIGF). An angiogenic factor (Ka) is calculated by formula: Ka=sFlt-1/PlGF×10. If Ka is 10 or less, the pregnant woman is stated to require no admission to hospital, no case follow-up; doctor's appointments are scheduled. If Ka falls within the range of 10 to 50, the pregnant woman is admitted to hospital, wherein foetal monitoring, Doppler monitoring are performed; an amniotic fluid index (AFI) is calculated; a therapy aiming at the uterine-placental blood flow improvement is prescribed for 10 days. The amount of infusion makes 400 ml a day. The prescribed preparations are Actovegin, Trental, Instenon, Carnitini chloridum. Control ultrasonography, Doppler monitoring, foetal monitoring, AFI and Ka measurements are performed 2 weeks later. The pregnant woman is discharged from hospital if observing no negative trends. If Ka falls within the range of 50 to 100, the pregnant woman is admitted to hospital, wherein foetal monitoring, Doppler monitoring are performed, and AFI is measured; a therapy aiming at the uterine-placental blood flow improvement is prescribed for 14 days The amount of infusion makes 800 ml a day. The prescribed preparations are Actovegin, Trental, Instenon, Carnitini chloridum. Control Doppler monitoring and foetal monitoring are performed every 3 days; 2 weeks later control Ka is measured. If the trend is positive, the pregnant woman may be discharged from hospital, while no positive trend requires another 2 weeks of the therapy. If Ka is 100 or more, but less than 150, the pregnant woman is admitted to hospital, wherein foetal monitoring, Doppler monitoring are performed, and AFI is measured; a therapy aiming at the uterine-placental blood flow improvement is prescribed for 14 days. The amount of infusion makes not less than 800 ml a day with the same preparations prescribed. Those are added with the preparations for homeostasis correction, including Fraxiparine, Fragmin, Clexane optionally. Control Doppler monitoring and foetal monitoring are daily. Hypamnion also requires measuring control AFI. If a gestational age is less than 34 weeks, respiratory distress syndrome (RDS) should be prevented by administering the preparation Dexon 24 mg according to the schedule: 6 mg every 12 hours 4 times. Control Ka is necessarily measured after 2 weeks of the treatment. If the trend is positive, the pregnant woman may be discharged from hospital, while no positive trend requires another 2 weeks of the therapy. If Ka is 150 or more, and the gestational age is more than 34 weeks, the therapeutic approach is the same, as for Ka being within 100 to 150, control Doppler monitoring, foetal monitoring are performed twice a day, as well as measuring AFI. If observing no foetal weight gain for 2 weeks of the therapy or the functional state of the foetus deteriorates, a Cesarean section is performed. If the gestational age is 34-36 weeks, the therapeutic approach and follow-up are the same as for the gestational age of 34 weeks, except for the prevention of foetal RDS. However, if observing the deterioration of a foetal movement pattern or the functional status of the foetus, a Cesarean section is performed according to the foetal monitoring and Doppler monitoring findings. If the gestational age is more than 36 weeks, and Ka is 150 or more, pre-mature delivery is applied.
 Method for prediction of developing primary open-angle glaucoma / 2517233
Lachrymal fluid and blood serum are analysed for the content of an anti-apoptotic Bcl-2 protein. If the same is not found in lachrymal fluid and/or serum, the developing glaucomatous process is predicted.
Method for prevention of perinatal central nervous system damage in newborn infants / 2517162
Invention refers to medicine, namely to paediatrics and neonatology, and may be used for prevention of a developing perinatal CNS damage of the hypoxic-ischemic genesis in infants of a risk group. That is ensured by evaluating birth-time umbilical blood neuron-specific enolase in an infant of a high perinatal risk group of the developing perinatal CNS damage of the hypoxic-ischemic genesis; if the derived value is more than 10 mcg/l, L-carnitine 2 drops 2 times a day and glycine 1/4 of a tablet 2 times a day are administered orally in the early neonatal period for the first month of life.
Method of estimating condition of blood coagulation system in patients in critical condition / 2517116
Invention relates to medicine and is intended for diagnosing disorders of homeostasis system. The following indices are determined: R1, MA1, G1, LY301, LY302, ΔR, ΔK, ΔAngle, ΔMA, ΔG and ΔLY30. If R1 is within the norm range, and ΔR is more than 9 min, deficiency of humoral factors of blood coagulation is diagnosed, in case of normal of MA1 and G1 indices, together with increase of parameters ΔMA and ΔG by more than 26 mm and 6 kDyn/cm2, deficiency of fibrinogen (factor I) is diagnosed, in case of more than 8% increase of index LY301 simultaneously with increase of index LY302 - activation of plasmin-dependent fibrinolysis, and in case of more than 8% increase of index LY301 with normal index LY302 - increased retraction of blood clot (hyperactivity of platelets).
Method of identifying prostate-specific antigen in liquid medium / 2517114
Invention relates to the field of medicine, namely to laboratory diagnostics, and can be applied for identification of the prostate-specific antigen (PSA) in liquid medium. For this purpose liquid medium interacts with a sensor, made in a form of a flat flexible cantilever. At least, one of the planes of the cantilever contains silicon dioxide and can reflect light irradiation. One of the planes is covered with bovine serum albumin. Another contains two layers, one of which is covalently bound with the surface of the cantilever, and the other contains chemically connected with the previous layer molecules of the antibody, specifically indentifying the prostate-specific antigen. After that, changes of the cantilever curve are determined by lightening the cantilever surface by a light beam and measuring deviation of the light beam, reflected form the cantilever surface. As a sensor applied is the cantilever, in which the layer, covalently bound with the surface of the cantilever, is made of 3-aminopropylsiltron.
Method for activation of white-cell-rich suspension growth and complex correction of blood composition in acoustic field in vitro / 2518534
Blood or its fractions are exposed to broadband oscillating acoustic signals at a temperature of 4°C to 38°C within the frequency range of 16-20000 Hz with a dominating level of noise pressure more than 45 dB, and less than 60 dB at 60-80 beats per minute, or to sequentially alternating acoustic signals with the dominating level of noise pressure ≤45 dB at <60 beats per minute and acoustic signals with the dominating level of noise pressure more than 65 dB, and less than 90 dB at >80 beats per minute.
|
FIELD: medicine. SUBSTANCE: with regard to a blood hormone value, a patient is exposed to a musical program selected taking into consideration the patient's musical preferences. Three algorithms are used for the exposure: with dominating acoustic pressure ≤45 dB and the rate <60 beats per minute (S algorithm); with dominating acoustic pressure more than 65 dB and less than 90 dB and the rate >80 beats per minute (T algorithm); with dominating acoustic pressure more than 45 dB and less than 60 dB, the rate of 60-80 beats per minute, or alternating S and T algorithms. EFFECT: method enables optimising blood hormones by increasing the body reserves and health through the adaptation reactions that has a favourable effect on the psychological and physical state of the people being tested, including their appearance. 4 tbl
The invention relates to medicine, relates to a method Wellness neurohormonal correction and rejuvenation with the use of musical and acoustic effects and can be used in various medical institutions. The closest is the way to healing the human body, which consists in the correction of psycho-emotional state of the patient by means of musical influence on him (EN 2143839, AB 5/04, publ. 10.01.2000). The known method the impact on the body is to record the action potentials, the formation of a sound effect depending on biosignal, the external sound influence form the polyphonic series, each audio component of which is determined by the corresponding biosignals, and the number of recorded action potentials determines the number of musical instruments for the execution of the respective parties. According to this method determines a preferred harmonics corresponding to the tone and the overtones of each biosignal, and based on the selected harmonics form the corresponding audio component. Sound effects form at least a two-channel acoustic system for regulating the phase of the sound effects of the subsequent channels relative to the previous one. Thisway, selecting for each biosignal musical instrument and synthesizing the appropriate party, carry out the creation and orchestration of the music of the body. Pre-adjustment of the original bio-measurement enables the creation of music, not only describing the state of the organism, but also allows you to adjust the physiological state of the organism. Carrying out a relative offset in time tunes of various bio-measurement, it is possible to obtain the effect of the spatial distribution and movement of sounds and accordingly adjust the direction of the effect on various processes in the biological object. The known method allows for the effects on the body with simultaneous consideration of multiple bio-measurement and their relationship. The disadvantage of this method is that the human body carry out external influence musical party within the receptive approach obedient listening to special music programs, based on bioelectric potentials translated into acoustic impacts on a particular principle. This music program is a set of sound vibrations, which is not music, it is music listener. In connection with this musical party becomes relevant physically the th patient's condition without regard to its overall mental state and mental state at the moment. At the same time found that the psycho-emotional state of the patient at a particular point in time may not correspond to the General state of the organism. In this case, the load of the patient's musical party does not have the desired effect, as it can not respond to his mood or state. Such exposure has on the patient's psycho-emotional overload with a negative reaction, not forming positive emotions in the patient. In addition, were not studied the consequences of such impacts from a psychophysiological point of view, in particular, for the hormonal system, which is significant not only from a scientific but also from a practical point of view. Achievable technical result is the restoration of neurohumoral regulation. The method is as follows. Prior exposure to determine the musicality of the patient, genre musical preferences, hemodynamic profile and levels of certain hormones in the blood. Further, in accordance with the received data, from music therapy music library make a compilation of musical works and form a program for neurohormonal correction and rejuvenation lasting 10 minutes or more. The library consists of various genres of musical works that use broadband oscillating justicesee signals in the frequency range 16-20000 Hz, each of which is based on hardware-acoustic analysis is divided into: 1) in the S-algorithm uses acoustic signals with dominant sound pressure level ≤45 dB, rate ≤60 beats/min, and applied to the identified increased blood levels of adrenaline, noradrenaline, cortisol, and reduced levels of serotonin and beta-endorphin. 2) in the T-algorithm uses acoustic signals with dominant sound pressure level of 65 dB, but less than 90 dB and the rate of >80 beats/min, is applied to the identified reduced blood levels of adrenaline, noradrenaline, cortisol, and increased levels of serotonin and β-endorphin; 3) in the HR-algorithm used acoustic algorithms, characterized by the dominant sound pressure level of more than 45 dB, but less than 60 dB, with a rate of 60-80 beats./min, or sequential alternating S - and T-algorithms for the correction of the level of adrenaline, noradrenaline, cortisol, serotonin and β-endorphin in the blood in the direction of the averages of normal (ideal standard). Treatments using favorites musical-acoustic effects are from 10 to 60 minutes is not less than 1 times a week for 2-3 weeks or more, the persons suffering from arterial hypertension, musical-acoustic stimulation of the T-algorithm is not assigned. To maintain the optimum level is ormanov in the blood for a long time and used in two technological contexts: in the form of audio listening, or a combination of audio listening, with additional simultaneous contact by impact of acoustic transmitters on the reflex zones of the skin. The most frequent indications for the use of the proposed methodology are: 1) functional after-stress disorder, 2) hormonal dysfunction, 3) neurotic disorders, 4) reduction in reserve capacity of the body, 5) premature aging and aging skin. For a comprehensive evaluation of the peculiarities of the influence on the human organism of the proposed method was implemented 2-stage research program. Materials and methods research 1-th stage. Main objective: to develop interventions using a variety of music-acoustic algorithms and to conduct a comparative study on the impact of one-time effects of various algorithms for up to 30 minutes on the levels of certain hormones, as well as on the ratio of the cellular elements of the white blood cells to assess the level of adaptive reactions. For research were selected 80 people of different age and sex with neurotic disorders after the stress and randomly divided into 3 experimental groups (AG-1, AG-2 and AG-3) - 20 people each and a control group (CG) the number is 20 people. For the implementation of acoustic effects we developed 3 musical-acoustic 30 minutes of playing each: No. 1 - using S-algorithms used in Aigues-1; No. 2 - using HR-algorithms used in Aigues-2; No. 3 - using T-algorithms - in Aigues-3. Patients KG listened to in the background randomly selected music one music radio for 30 minutes. Blood for research of a level of hormones and cellular composition of cells were taken from the cubital vein in patients of all groups twice: on an empty stomach to musical and acoustic impact and immediately after it. Methods. For acoustic measurements experimental musical-acoustic software was used generic instrument Akathisia-110A (Russia). The study of the dynamics of the hormonal status was carried out by immunochemical method using an analyzer Architect i1000, the blood has been investigating the level of β-endorphin, serotonin, cortisol, epinephrine and norepinephrine. For studies of the morphological composition of the blood used Hematology analyzer Sysmex XE-2100, Sysmex Corporation (Japan). Interpretation of results was performed in accordance with the methodological recommendations on the activation therapy (Garkavi LH, 2006). The results of the research education who was aivalis methods of variation statistics. When comparing the distributions of different indicators for different groups used student test for small samples - Li-test Mann - Whitney. Research results and discussion Formed by random selection of experimental and control groups were comparable by age and gender indicators. The effects of different algorithms of acoustic effects on hormone levels in the blood are shown in table 1.
As can be seen from the data presented in table 1, sedative music-acoustic stimulation (S-algorithm) caused a significant increase in the group EG-1 average values of indicators of serotonin 1.9 times (p≤0,05), thus lowering the level of blood adrenaline, noradrenaline (p≤0.05) and cortisol (p≤0,01). β-endorphin showed implausible fluctuations with the General trend towards a slight decrease in the level of content in the blood. Visually and according to the survey activity while patients EG-1 was decreased, there were muscular and emotional relaxation, a feeling of drowsiness. In the group EG-2, after the musical-acoustic stimulation using the HR-algorithm, has been a significant increase in the level of blood adrenaline 1.8 times (p≤0,05). β-endorphin, norepinephrine, and cortisol showed a General trend to some higher level content in the blood (p≥0.05), and serotonin - to decrease. In the quantitative analysis of the resulting figures, revealed the dynamics optimization of hormonal background, i.e. the tendency in the movement level of the studied hormones to the average values of the corridor is army. In this way, the individual was noted after the procedure even slightly good mood and good enough performance. In the group EG-3, after applying the T-algorithm musical-acoustic impacts occurred reliable change indices of all the studied hormones: increased level of blood adrenaline 2.3 times (p≤0.01), and norepinephrine 1.2 times (p≤0,05), cortisol 1.4 (p≤0.05) and β-endorphin 1.2 times (p≤0,05). Serotonin levels were decreased by 1.6 times (p≤0,05). Subjectively, the subject was noted after the procedure, feeling the tide of physical strength, a significant rise of mood and activity. The most sensitive hormone musical-acoustic effects in our studies was the adrenaline, which level in the blood was significantly changed after using all algorithms impact. In the control group significant change in hormone levels in the blood not detected. As a result of laboratory studies have established certain changes in the composition of white blood cells. The results obtained are shown in table 2.
It is revealed that significant changes occurred in the level of lymphocytes (p the article) and segmented leukocytes (decrease) in all experimental groups. After MAB-S index value of LY/SEG in Aigues-1 increased up to a level of 0.57 (reaction peaceful activation), and after MAB-HR in Aigues-2 index LY/SEG rose to a value of 0.75 (reaction high activation). In Aigues-3 index value of LY/SEG rose to the level of 0.61 (reaction peaceful activation)and in KG index fluctuations LY/SEG were insignificant. Thus, all the algorithms MAV caused a reaction of adaptation, which, as is well known (Garkavi LH, 2006), increase the reserve capacity of the organism and health. It is shown that the most positive leukocyte changes in the peripheral blood occurred after conducting MAB-HR, when the sedative music-acoustic stimulation alternated with neutral or mildly tonic, was observed optimize hormonal levels, i.e. the tendency in the movement level of the studied hormones to the averages of the normal range. Growth index values LY/SEG to 0.75 demonstrated the development of the reaction increased activation. Materials and methods the 2nd stage of the research. The main objective of the 2nd phase of the study was the development of music therapy programs using HR-algorithms and comprehensive assessment of the peculiarities of the influence of music therapy influences the emotional sphere and the condition of the skin in persons with impaired microcirculation of the skin. Surveyed 3 women aged 20 to 40 years with various signs of disturbances of microcirculation and trophism of the skin. All subjects were patients randomly were divided into 2 groups. The main group of 20 people was conducted Meso-Forte therapy: 5 procedures for 15 minutes every other day. The procedure was carried out as follows - in the face dressed cloth mask, impregnated with a bioactive mixture, consisting of: distilled water, shark oil, extracts of olive leaf, seaweed, placentae, vitamins a, C, E. the Free ends of a fabric mask with two sides were brought to the projection of the ears and covered them. Then top dressed Studio stereo headphones and within 15 min on each procedure was broadcast one of the music therapy programs: "anti-Stress & Insomnia, Depression & Fatigue", "Therapy of fear and anxiety, Hypertension & Nervous stress", "the Breath of life & Neurasthenia" series "Music health". Thus, acoustic signals was affected in two ways: on the auditory analyzer and right on the cells of the skin through the fabric, part of which was under headphones, and performed along with the function of bioactive mask at the same time the role of the acoustic membrane, spreading coming from headphones audio signals on covered skin surface. The control group of 10 people was carried out by Issa the procedures similar chemical composition, frequency and duration, but without musical influence. In a comprehensive research program has included medical and psychological tests. 1) a Survey of complaints and clinical observation was permanent in the course of the program. 2) To measure the level of anxiety, which is used twice, before treatment and after the scheduled course, used a questionnaire Taylor consisting of 50 statements, adapted T.A. Neminem in 1966. 3) a Comprehensive study of blood flow in subcutane vessels using laser Doppler flowmetry was performed using a skin contact sensor on the device BLF-21 firms Transonic Systems (USA). Laser Doppler flowmetry (LDF) is a sensitive and informative diagnostic method allowing to assess the state of the microcirculation, which has a direct influence on the trophism of tissues and organs, as well as on the activity of regeneration processes in various pathological processes, including inflammatory and degenerative. Measurement of the Doppler components in the spectrum of the reflected laser signal scattered by moving in the tissues of the particles, allows using LDF non-invasive way to assess the volume of blood flow (per unit time through a unit mass of tissue), for example, set the te simultaneous or dynamically capillary blood flow in the superficial layers of the skin. Was carried out three-time measurement of blood flow with subsequent averaging: for 5 minutes before the start of the 1st procedure, after the 1st procedure and after finishing all the procedures. 4) Objective assessment of the condition of the skin was carried out using a computer program SkinXPro that determines the degree of humidity, the excretion of fat, smooth, pigmentation. The results of all studies were processed by methods of variation statistics. When comparing the distributions of different indicators for different groups were used U - test Mann-Whitney. Mathematical processing of the investigated material was carried out using software tools "Statist". The results obtained. In the clinical examination, the patients and control group defined the symptoms of skin microcirculation. Revealed - pallor, dryness, smoothness of skin image. Found that 83.3% of the surveyed individuals connect their existing skin problems with emotional stress and chronic nervous stress. With 75% of the main group and 80% of controls showed a variety of complaints neurotic character, among which the most frequent were: fear, sleep disturbance and depressed mood. Dynamics of claims after procedures are presented in table 3.
In accordance with the presented data revealed, in the study group occurred a significant decrease in the number of neurotic complaints by 50% (U=<0.01). In the control group the reduction of complaints by 10% was not significant (U>=0.05). When assessing personal property on the anxiety scale Taylor found that the average values of indices of anxiety in all examined groups initially hesitated in the middle level with a tendency to high, namely in the main group - 25.5±7.2 points, control 27±7.7. It is established that at the end of the observations statistically significant decrease in the average value of an indicator of anxiety occurred in the primary group at 21.6% (U=<0.05). In the control group repeated testing revealed a nonsignificant decrease in anxiety by 3.7%. When assessing the physiological state of the skin found that before treatment in all examined groups there was a decrease subcutaneo blood flow. In the main group the value of this indicator was 8.5±2.31, control and 7.9±1.8 (at a rate equal to 15.6±4,1 ml/100g/min). Dynamics subcutaneo blood flow after a series of treatments is presented in table 4.
In accordance with the presented data shows that in the main group after the first procedure indicator subcutaneo blood flow significantly increased 2.3 times from baseline at the level of values U=<0,001, and after 5 treatments consistently normalized, reaching a value of 16.5±3.0 ml/100g/min (U=<0,01). In the control group after 5 beauty masks indicator subcutaneo inaccurate blood flow increased 1.4, remaining at the level of values below normal. Improvement of the physical condition of the skin revealed in the main and control groups. However, the degree of hydration, the maximum increased in the study group with 29,7±4.9 to 36.4±6.2 cu (U-value=0.01). The smoothness of the skin increased in both groups, the maximum dynamics in the main, where she was 43.3±2,1 $ U=<0,01). Patients in both groups improved elasticity and skin color but more pronounced positive dynamics was in the main group. What are the mechanisms of the results? It is known that stress can negatively affect the skin condition. So in the 1st stage of the stress out adrenaline. In the body, the reaction begins mobilization: increased blood pressure and heart rate, redistributed blood. The skin begins to feel the lack of oxygen, what becomes unhealthy yellowish hue. In the 2nd stage of stress increases production of the hormone cortisol, which suppresses the immune system. In the skin glucocorticoids in high concentrations inhibit the growth and division of fibroblasts, which leads to the thinning of the skin, its light damage, poor healing of wounds. The skin becomes susceptible to infections. Stress may have a negative impact on digestion which leads to accumulation of toxins, including in the skin, which can lead to rashes and inflammatory processes. Therefore, combating stress and its effects is a prerequisite for successful recovery of the skin or treatment of dermatological patients. The perception of pleasant melodies by ear has anti-stress effect, soothes, relieves spasms of peripheral blood vessels, including capillaries. In nikeysha optimization level of hormones in the blood when using HR-algorithms musical-acoustic effects contributes to the balance of the processes of inhibition and excitation in the Central nervous system, balances the activity of the sympathetic and parasympathetic divisions outside. In General, the proposed method Wellness neurohormonal correction and rejuvenation with the help of music-acoustic effects allows you to optimize the content of the level of hormones in the blood, increasing the reserve capacity of the organism and the level of health with the help of reactions adaptation, which ultimately has a positive impact on psychological and physical state of the patient, including the appearance of the patient. 1. The way neurohormonal correction using musical-acoustic effects, including the determination of hormone levels in the blood and genre of the musical preferences of the patient, on the basis of revealed preferences select music, forming the musical program, then taking into account the identified level of hormones carry out impact, using acoustic signals: from the dominant sound pressure level ≤45 dB and rate <60 beats./min (S-algorithm) - elevated levels of epinephrine, norepinephrine, cortisol, and reduced levels of serotonin and β-endorphin; with the dominant sound pressure level of 65 dB, but less than 90 dB and the rate of >80 beats./min (T-algorithm) - at low levels of epinephrine, norepinephrine, cortisol, and increased levels of serotonin and β Endorf is on; dominating the sound pressure level of more than 45 dB, but less than 60 dB, with a rate of 60-80 beats./min, or sequential alternating S - and T-algorithms - if necessary, adjust the levels of epinephrine, norepinephrine, cortisol, serotonin and β-endorphin in the direction of the average values of the norms of these hormones. 2. The method according to claim 1, characterized in that the duration of the music program is from 10 minutes or more. 3. The method according to claim 1, characterized in that the influence exercised by not less than 1 times a week, for two weeks or more. 4. The method according to claim 1, characterized in that the impact is realized by means of audio listening. 5. The method according to claim 1, characterized in that the impact carried out through a combination of audio listening, with a simultaneous contact of the impact of acoustic transmitters on the reflex zones of the skin.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||