Method of culturing bifidobacteria in milk
SUBSTANCE: invention relates to biotechnology and can be used in the dairy industry. Pasteurised milk is cooled to a given temperature and MnSO4, ZnSO4, KJ, CuSO4, FeSO4 and selexen are then added in a given ratio, followed by addition of bifidobacteria. The mixture is stirred and soured until a clot of given titratable acidity is obtained.
EFFECT: improved method.
1 tbl, 1 ex
The invention relates to biotechnology and can be used in dairy industry for the production of fermented milk products with bifidobacteria.
A known method of growing bacteria (Patent RU 2205217 C1, 2003), proposing to skim milk as a stimulator of the growth of bifidobacteria in whey syrup in the amount of 5.0-6.0% by weight of milk produced by fermentation of clarified condensed serum β-galactosidase.
The disadvantage of this method of cultivation is the length of the process, getting a high enough number of bifidobacteria and a significant increase in the carbohydrate component of the obtained product.
Closest to the invention to the technical essence and the achieved result is the method of cultivation of bifidobacteria (Patent RU 2375442 C2, 2009), where as a stimulator of the growth of bifidobacteria during culturing them in the non-fatty milk use natural mixture of oligofructose and inulin - Beneo™Synergy 1 (BS1).
The disadvantage of using this method of cultivation in industrial environments is the high cost growth stimulator.
The objective of the invention is development of method of cultivation of bifidobacteria in milk using as a growth stimulant mixtures of alimentary-acceptable mineral and body is ical compounds, cost component in which the unit of finished product is lower than in the equivalent.
The technical result of the invention is to improve the reproductive properties of bifidobacteria when grown in milk.
The essence of the invention lies in the fact that the quality of the stimulator for bifidobacteria used a mixture of mineral and organic compounds: manganese sulfate, zinc sulfate, iron sulfate, copper sulfate, potassium iodide and selexin (selenolate).
One of the major reasons of the stimulating effect of manganese associated with nucleic acids that are part of many compounds that increase the enzymatic activity. Zinc stimulates the synthesis of cellular protein due to the intensive involvement of nitrogen in synthetic reactions reinforce the processes of fission. Potassium and copper contribute to the utilization of glucose for growth and reproduction of bacteria. Selenium is actively involved in the biochemical and physiological processes of organisms (in some sources of selenium compounds - SELENATES indicate both antimetabolites. In addition, one of the protein components of microorganisms is, Selenomethionine). It is also known that the bacteria are present in proteins containing in its active center iron.
Strains of Bifidobacterium longum L. and Bifidobacterium longum D-1 obtained from the Museum's collection to clean is ltur microorganisms Vologda state milk Academy. N.V. Vereshchagin.
Microorganisms cultured pasteurized fat-free milk, to which add a splash of mineral and organic compounds, each separately, in doses of from 0.2 to 3 mg/DM3.
The invention is illustrated by the following examples.
The proposed method uses a milk subjected to pasteurization at a temperature of (87±2)°C with exposure (10-15) minutes, cooled to 37°C and used as a nutrient medium for cultivation of bifidobacteria.
Strains of Bifidobacterium longum seeded in pasteurized milk in quantities of 5% of skim milk.
Mineral and organic compounds are introduced into the milk, each separately, to the desired concentration, and mineral salt previously dissolved in distilled water and subjected to boiling, and selexin dissolve in butter, heated to a temperature of 60-80°C. the Fermented mixture is stirred and sours at a temperature of 37°C until the formation of a clot (titratable acidity 60°T). Control options do not contain additives of mineral and organic compounds.
The quantification of bifidobacteria was conducted by the method of limiting dilutions and inoculation to a standard corn-lactose medium MMC-1.
Technological assessment of the impact of a mixture of mineral and organic compounds spent the duration of the fermentation and the number of bifidobacteria.
The data given in the table.
The obtained results show that the mixture of mineral and organic compounds have a stimulating effect on the growth of bifidobacteria under cultivation in milk at concentrations mg/DM3: MnSO4- (0,25±0,05); ZnSO4- (3±0,2); KJ - (2,5±0,1); CuSO4- (2,0±0,1), FeSO4- (1,0±0,1), seleken - (0,2±0,05)
|The effect of a mixture of mineral and organic compounds on the growth of bifidobacteria in milk|
|The analyzed strains cultures of bifidobacteria||Reducing the time of ripening in comparison with control, %||The change in the number of bacteria in comparison with control, ln CFU/cm3|
|Bifidobacterium longum L||15,9||+1,41|
|Bifidobacterium longum D-1||16,9||+2,23|
Sources of information
1. RF patent №2205217, 2003.
2. RF patent №2375442, 2009.
The method of cultivation of Bifidobacterium Bifidobacterium longum in milk, wherein the milk to make a mixture of alimentary-acceptable mineral and organic compounds, the content of which is, mg/DM3:
KJ - (2,5±0,1);
Seleken - (0,2±0,05).
FIELD: medicine, pharmaceutics.
SUBSTANCE: claimed invention relates to field of microbiology and deals with method of obtaining RTX-toxin ApxI. Claimed method is realised by cultivation of bacteria Actinobacillus pleuropneumoniae in culture medium, which provides growth of bacteria, and said culture medium contains borogluconate in concentration lower than 60 mmol/l in order to form in medium calcium-bologluconate complex.
EFFECT: invention makes it possible to increase output of RTX-toxin ApxI, which can be applied in production of vaccines.
5 cl, 4 tbl
SUBSTANCE: invention relates to biotechnology and can be used to produce thermolabile enterotoxin (LT-enterotoxin) and Hafnia alvei anatoxin when producing a vaccine. The strain is deposited in the State Collection of Pathogenic Microorganisms of FBSI Scientific Centre for Evaluation of Medical Products of the Ministry of Public Health and Social Development of Russia under number 294.
EFFECT: strain has high capacity for producing thermolabile LT-enterotoxin.
1 tbl, 2 ex
SUBSTANCE: biological preparation comprises the culture fluid containing the strain of nitrogen-fixing nodule bacteria with a titre of not less than 107 CFU/ml, and an aqueous solution containing (wt %): sodium carboxymethyl cellulose (2.0-5.0), potassium sorbate (0.5-3.0) and glycerol (1.0-5.0), mixed in a ratio of from 1:1 to 1:2.
EFFECT: invention enables to increase the productivity of leguminous crops and to increase the shelf life of the resulting preparation.
3 tbl, 2 ex
SUBSTANCE: invention refers to microbiology, particularly to the protection of agricultural, medicinal and forest crops. The strain Bacillus thuringiensis var. thuringiensis No.800/15 (BtH1 800/15) possesses the entomocidal activity on depredators - phytophage insects. The strain is deposited in State Scientific All-Russia Research Institute for Agricultural Microbiology (GNU VNIISHM), No. 611.
EFFECT: strain may be used in preparing the high-efficacy entomocidal biopreparation.
3 tbl, 1 ex
SUBSTANCE: strain Bacillus thuringiensis var. darmstadiensis №25 (BtH10 №25) has insecticidal activity against pests - coleopteran insects and a wide range of antifungal activity against pathogenic fungi. It is deposited in State Scientific Institution All-Russia Institute for Agricultural Microbiology under the registration number RCAM01490. It can be used in the manufacture of polyfunctional means of protection of plants against harmful coleopteran insects and phytopathogenic fungi.
EFFECT: invention also enables to improve the germinating capacity of plants.
7 tbl, 1 ex
SUBSTANCE: strain of bacteria Bacillus atrophaeus VKPM V-10592 is grown, and suspension is prepared from it, which is introduced into permafrost soil and water environment. Maintained at the specified parameters from 7 to 60 days, and then they determine quantity content of oil and oil products in permafrost soil and water environment.
EFFECT: invention makes it possible to reduce time for oil and oil product denaturation and to reduce their concentration in permafrost soil and water environment.
5 tbl, 6 ex
SUBSTANCE: invention relates to biotechnology. Claimed is method of obtaining androst-4,9(11)-dien-3,17-dione from phytosterol. Microbiological oxidative elimination of side chain at atom C17 with formation of 9α-hydroxyandrost-4-en-3,17-dione is performed. Biomass is separated. 9α-hydroxyandrost-4-en-3,17-dione is extracted from clarified cultural liquid with aprotic organic solvent, selected from aromatic hydrocarbons or organochlorine hydrocarbons. After that, reaction of 9α-hydroxygroup of 9α- hydroxyandrost-4-en-3,17-dione dehydration is carried out in obtained extract. As dehydration agent applied is mineral acid, which contains water and is selected from group, which includes orthophosphoric, pyrophosphoric and chloric acids. Mineral acid is applied in quantity from 1 to 10 mol per 1 mol of 9α- hydroxyandrost-4-en-3,17-dione. In the process of dehydration reaction removal of excessive water is carried out either in presence of effective quantity of pyrophosphoric acid or by azeotropic distillation.
EFFECT: invention makes it possible to intensify dehydration process with application of smaller quantity of mineral acid and exclude side product formation.
11 cl, 1 tbl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology, microbiology, and concerns the recovery and identification of pseudotuberculosis and intestinal yersiniosis agents (Y. Pseudotuberculosis and Y. Enterocolytica). A nutrient medium contains microbiological agar (dry), lactose, glucose, urea, calcium chloride, 1% alcoholic phenol red, 1% alcoholic methylene blue and distilled water in specific proportions.
EFFECT: invention enables reducing the length of studies.
3 dwg, 2 tbl
SUBSTANCE: growing of Staphylococcus aureus is carried out in a nutrient medium containing yolk-salt agar. At a stage of preparation for analysis the growth stimulators of Staphylococcus aureus are introduced into the nutrient medium in the form of aqueous solutions at concentrations of 10-4-10-6 wt %. The following compounds are used as growth stimulators: tris(2-hydroxyethyl)ammonium 4-chlorophenyl-sulfanylacetate or tris(2-hydroxyethyl)ammonium 2-chlorophenyloxyacetate or tris(2-hydroxyethyl)ammonium 2-methyl-4-chlorophenyloxyacetate or tris(2-hydroxyethyl)ammonium 1-benzylindol-3-yl-sulfanylacetate.
EFFECT: invention enables to accelerate growing of Staphylococcus aureus for diagnostics of infections, by reducing the time of growing from 48 to 6-9 hours in comparison with the control in a standard nutrient medium.
2 tbl, 7 ex
SUBSTANCE: invention is production of the nutrient medium, which creates optimal conditions for growing legionella, comprising: enzymatic hydrolyzate of pig lung, enzymatic hydrolyzate of chicken egg yolk, potassium monophosphate, trihydrate disubstituted potassium phosphate, L-cysteine hydrochloride, activated carbon, microbiological agar and distilled water at a predetermined ratio of ingredients.
EFFECT: invention enables to produce the high-quality, easy-to-prepare nutrient medium, to reduce the time of growing legionella.
FIELD: food industry.
SUBSTANCE: milk mixture is prepared from defatted milk and butter milk at a ratio of 1:1-2:1. The milk mixture is pasteurised at a temperature of 90-95°C, maintained during 2.5-3 hours and cooled to a fermentation temperature. A lactic acid microorganisms starter is introduced; the starter is represented by a combined starter of bifidobacteria, lactococci and leuconostoc cultures at a ratio of 3:1:1; the mixture is ripened at a temperature of 30-34°C till formation of a clot with acid content equal to 80-85°T which is dehydrated and cooled.
EFFECT: manufacture of a creamy coloured product with increased nutritive and biological value, a soft crumbly consistency which allows the product to be similar to the Tartar national product kort in terms of organoleptic properties.
3 tbl, 4 ex
FIELD: food industry.
SUBSTANCE: invention relates to dairy industry. A milk-and-vegetal mixture consisting of defatted milk and 2-5% whole-ground amaranth flour is standardised, purified, homogenised, pasteurised and cooled to the fermentation temperature. One adds a starter containing Streptococcus thermophilus and Lactobacillus delbrueckii subsp. Bulgaricus yoghurt cultures and Bifidobacterium adolescentis MC-42 strain at a ratio of 1:15 - 1:30, ripens the mixture at a temperature of 37-39°C during 5-9 hours till acid content is equal to 92-100°T, stirs the mixture, cools it and performs dispensing.
EFFECT: manufacture of a product with preventive, dietary, probiotic and functional properties.
2 tbl, 3 ex
FIELD: food industry.
SUBSTANCE: milk is standardised in terms of fat content; soluble dry low-calc coprecipitate is introduced. One proceeds with filtration and Ecolact milk fat substitute introduction, emulsification, introduction of cream with 25% fat content, homogenisation, pasteurisation and cooling to a fermentation temperature. Fermentation is performed using Bifidobacterium bifidum bifidobacteria antagonistic strain starter; antioxidants (alcohol solution of dihydroquercetin and ascorbic acid) are introduced; one proceeds with ripening and placement onto pans. Freezing and sublimation drying are performed.
EFFECT: invention is aimed at production of a product with high food and biological value and extended useful life, extension of the range of dry dairy preserves that have enhanced food and biological value and extended useful life.
2 tbl, 3 ex
FIELD: food industry.
SUBSTANCE: method involves standardisation, pasteurisation, homogenisation, cooling to fermentation temperature, introduction of kefir fungi based industrial starter in an amount of 2% simultaneously with transglutaminase enzyme in an amount of 0.2 kg per 1000 kg of the standardised mixture at a temperature of 23-25°C, ripening, cooling to maturation temperature, bottling. The content of lactoferrin in the ready product is 500-1100 mg/l.
EFFECT: invention allows to enhance the product biological value and prophylactic properties, prevent synaeresis in the process of storage, enhance the product rheological properties, produce a product with improved structurem and enhanced quality, a milder fermented milk taste and aroma, impart creaminess to the product and extend its storage life.
SUBSTANCE: invention relates to microbiology and biotechnology. Disclosed is a consortium of Lactobacillus rhamnosus VKM B-2726D and Lactobacillus plantarum VKM B-2725D strains.
EFFECT: consortium is used to produce a bacterial preparation and a direct administration ferment for producing fermented milk and fermented beet juice as functional nutrition products.
15 tbl, 13 ex
SUBSTANCE: strain Lactococcus casei VKPM V-8730 is obtained at available growth-supporting media and is used as starter material in cultured milk products production.
EFFECT: invention allows reducing the time of technical process and increasing cultured product shelf life.
SUBSTANCE: strain Enterococcus durans VKPM B-8731 is obtained at available growth-supporting medium and is used as starter material in cultured milk products production.
EFFECT: invention allows reducing final acidity of cultured milk product, reducing the time of technical process and increasing cultured product shelf life.
SUBSTANCE: invention pertains to the use of L. casei ssp. paracasei CNCM 1-1518 strain for giving antimycotic properties to fermented dairy product. Inhibiting of mould development of ascomycete class in the said product is made by dairy substrate fermentation in presence of this strain for, at least, 5 hours. Thallome diameter after 7 days of incubation amounts 8.8±1 mm.
EFFECT: increase of antimycotic activity in fermented dairy product.
6 cl, 3 tbl, 4 ex
SUBSTANCE: method for preparing an autoprobiotic containing living bifidus bacteria and lactic acid bacilli for the rehabilitation of human and animal intestinal microflora involves the recovery of living bifidus bacteria and lactic acid bacilli from a host's intestine and the use thereof in the form of automicroorganisms with the above bifidus bacteria and lactic acid bacilli recovered simultaneously in the form of a natural complex of indigenous microorganisms by dissolving the host's intestinal contents with a fluid with adding selective ingredient having no inhibitory action on the bifidus bacteria and lactic acid bacilli, adding a solution of the same host's saliva, culturing the mixture and inoculating a nutrient medium for culturing the bifidus bacteria and lactic acid bacilli with no selective agents added.
EFFECT: invention provides the recovery of microbiocoenosis of all strains of the bifidus bacteria and lactic acid bacilli simultaneously in the form of the natural complex of the indigenous microorganisms and the more effective rehabilitation of the normal microflora in the host's body.
SUBSTANCE: group of inventions refers to microbiology and biotechnology. Proposed strains Lactobacillus crispatus "ВКМ" B-2727D, Lactobacillus gasseri "ВКМ" B-2728D and Lactobacillus plantarum "ВКМ" B-2731D have antagonistic activity in relation to pathogenic and semi-pathogenic microorganisms. Besides, the invention proposes a consortium based on the above strains for production of a bacterial preparation, a biologically active additive, and direct inoculation for obtaining fermented milk product of functional nutrition.
EFFECT: consortium has higher antagonistic activity against pathogenic and semi-pathogenic microorganisms in relation to individual strains of lactobacilli, use of a consortium will allow enlarging the range of devices intended for prophylaxis and treatment of urogenital infections of females.
4 cl, 1 dwg, 7 tbl, 12 ex
FIELD: milk industry, in particular, production of lactic-acid bifidus activating product for grownups and at least one-year-old children.
SUBSTANCE: method involves pasteurizing whole milk; cooling to fermentation temperature; introducing starter including bifidus bacteria and lactic streptococcuci; introducing vitamin additive; mixing; fermenting until live cell content in product is 109-1010 CFU/cm3 and acidity is 80-1000T; cooling; mixing and bottling. Vitamin additive is concentrated citrus juice, or Jerusalem artichoke juice, or Jerusalem artichoke powder juice, or Jerusalem artichoke syrup. Concentrated citrus juice is introduced in an amount of 30-50 ml per 1 l of milk, Jerusalem artichoke juice, powder juice or syrup is introduced in an amount of 0.3-0.5% by volume of milk. Method allows useful components to be extracted to maximal extent from basic product.
EFFECT: improved quality, increased assimilation of useful components by people of any age owing to development of natural microflora by product itself.
5 tbl, 5 ex