Method of culturing bifidobacteria in milk

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and can be used in the dairy industry. Pasteurised milk is cooled to a given temperature and MnSO4, ZnSO4, KJ, CuSO4, FeSO4 and selexen are then added in a given ratio, followed by addition of bifidobacteria. The mixture is stirred and soured until a clot of given titratable acidity is obtained.

EFFECT: improved method.

1 tbl, 1 ex


The invention relates to biotechnology and can be used in dairy industry for the production of fermented milk products with bifidobacteria.

A known method of growing bacteria (Patent RU 2205217 C1, 2003), proposing to skim milk as a stimulator of the growth of bifidobacteria in whey syrup in the amount of 5.0-6.0% by weight of milk produced by fermentation of clarified condensed serum β-galactosidase.

The disadvantage of this method of cultivation is the length of the process, getting a high enough number of bifidobacteria and a significant increase in the carbohydrate component of the obtained product.

Closest to the invention to the technical essence and the achieved result is the method of cultivation of bifidobacteria (Patent RU 2375442 C2, 2009), where as a stimulator of the growth of bifidobacteria during culturing them in the non-fatty milk use natural mixture of oligofructose and inulin - Beneo™Synergy 1 (BS1).

The disadvantage of using this method of cultivation in industrial environments is the high cost growth stimulator.

The objective of the invention is development of method of cultivation of bifidobacteria in milk using as a growth stimulant mixtures of alimentary-acceptable mineral and body is ical compounds, cost component in which the unit of finished product is lower than in the equivalent.

The technical result of the invention is to improve the reproductive properties of bifidobacteria when grown in milk.

The essence of the invention lies in the fact that the quality of the stimulator for bifidobacteria used a mixture of mineral and organic compounds: manganese sulfate, zinc sulfate, iron sulfate, copper sulfate, potassium iodide and selexin (selenolate).

One of the major reasons of the stimulating effect of manganese associated with nucleic acids that are part of many compounds that increase the enzymatic activity. Zinc stimulates the synthesis of cellular protein due to the intensive involvement of nitrogen in synthetic reactions reinforce the processes of fission. Potassium and copper contribute to the utilization of glucose for growth and reproduction of bacteria. Selenium is actively involved in the biochemical and physiological processes of organisms (in some sources of selenium compounds - SELENATES indicate both antimetabolites. In addition, one of the protein components of microorganisms is, Selenomethionine). It is also known that the bacteria are present in proteins containing in its active center iron.

Strains of Bifidobacterium longum L. and Bifidobacterium longum D-1 obtained from the Museum's collection to clean is ltur microorganisms Vologda state milk Academy. N.V. Vereshchagin.

Microorganisms cultured pasteurized fat-free milk, to which add a splash of mineral and organic compounds, each separately, in doses of from 0.2 to 3 mg/DM3.

The invention is illustrated by the following examples.

Example 1

The proposed method uses a milk subjected to pasteurization at a temperature of (87±2)°C with exposure (10-15) minutes, cooled to 37°C and used as a nutrient medium for cultivation of bifidobacteria.

Strains of Bifidobacterium longum seeded in pasteurized milk in quantities of 5% of skim milk.

Mineral and organic compounds are introduced into the milk, each separately, to the desired concentration, and mineral salt previously dissolved in distilled water and subjected to boiling, and selexin dissolve in butter, heated to a temperature of 60-80°C. the Fermented mixture is stirred and sours at a temperature of 37°C until the formation of a clot (titratable acidity 60°T). Control options do not contain additives of mineral and organic compounds.

The quantification of bifidobacteria was conducted by the method of limiting dilutions and inoculation to a standard corn-lactose medium MMC-1.

Technological assessment of the impact of a mixture of mineral and organic compounds spent the duration of the fermentation and the number of bifidobacteria.

The data given in the table.

The obtained results show that the mixture of mineral and organic compounds have a stimulating effect on the growth of bifidobacteria under cultivation in milk at concentrations mg/DM3: MnSO4- (0,25±0,05); ZnSO4- (3±0,2); KJ - (2,5±0,1); CuSO4- (2,0±0,1), FeSO4- (1,0±0,1), seleken - (0,2±0,05)

The effect of a mixture of mineral and organic compounds on the growth of bifidobacteria in milk
The analyzed strains cultures of bifidobacteriaReducing the time of ripening in comparison with control, %The change in the number of bacteria in comparison with control, ln CFU/cm3
Bifidobacterium longum L15,9+1,41
Bifidobacterium longum D-116,9+2,23

Sources of information

1. RF patent №2205217, 2003.

2. RF patent №2375442, 2009.

The method of cultivation of Bifidobacterium Bifidobacterium longum in milk, wherein the milk to make a mixture of alimentary-acceptable mineral and organic compounds, the content of which is, mg/DM3:
MnSO4ZnSO4- (3,00±0,2);
KJ - (2,5±0,1);
CuSO4- (2±0,1);
FeSO4- (1±0,1);
Seleken - (0,2±0,05).


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