Method of treating endometritis in cows

FIELD: medicine.

SUBSTANCE: invention relates to veterinary science. There are involved the intrauterine infusions of the ozonised emulsion 100.0 ml consisting of equal portions of cod liver oil and linseed infusion every 48 hours until clinical recovery is observed.

EFFECT: method is simple and high-effective in treating endometritis in cows.

9 tbl, 1 ex

The invention relates to veterinary medicine, namely to the veterinary obstetrics, and can be used for the treatment of endometritis in cows.

Known methods, techniques, and patterns [1, 2] treatment of endometritis: causal, pathogenesis, symptoms, physiotherapy, mud, denaturate and others. The basis of a comprehensive treatment of animals with inflammatory processes in the reproductive organs are antimicrobials. As antimicrobial agents used antibiotics, sulfonamides, nitrofurans and other antiseptic.

The disadvantages of their application include high cost, low efficiency because of the narrow focus with respect to microorganisms, negative impact on the performance of nonspecific resistance of the organism and on the morphofunctional state of the mucous membrane of the uterus, and on the background of immunodeficiency of the host they can cause relapse and recurrence of the carrier state, occurrence in nature, highly resistant strains of microorganisms and inflammation fungal etiology. It is established that in any way the introduction of antibiotics, sulfonamides and nitrofurans can accumulate in the organism of animals and a long period of time stored in animal products. Antibiotics and sulfonamides, opada in the body of the animal and human can cause an allergic reaction, anaphylaxis, a change in the quantitative and qualitative composition of the bacterial microflora and other undesirable phenomena.

The known method [2] etiotropic therapy of cows suffering from endometritis, which is based on intrauterine injection of the emulsion godisgoodalltime at a dose of 50-100 ml.

The disadvantage of this method of treatment is, first of all, the deficit on the market, high cost, lack of therapeutic efficacy, the need for rejection of milk during treatment and for 2 days after the disappearance of clinical signs of disease.

The essence of the proposed method lies in the fact that for the treatment of endometritis in cows offered General similar to the prototype sign - etiotropic therapy. In relation to the prototype hallmark is the use of ozonated emulsion. Preparing an emulsion of equal parts of fish oil and extract of flax seed (obtained by infusion within 30 minutes of flax seeds in boiled water at a ratio of 1:30). Ozonation of the emulsion is carried out with the use of ozone in ozone concentration at the outlet of the device 20 mg/l for five hours, which is later used for the treatment of endometritis in cows by intrauterine injections.

Held a number of Toxicological studies using the m ozonated emulsion. To determine the acute toxicity of ozonated emulsion was diluted with sterile saline solution from step 2. Each dilution homogenized until a homogeneous emulsion was injected intraperitoneally white mice.

The maximum of the injected doses were 0.5 ml, which exceeded more than 20 therapeutic doses. The amount of a therapeutic dose was calculated based on the planned therapeutic doses for cows (50 ml), taking into account the ratios of mass and surface area of the animal body.

After the introduction of the tool after the animals were observed for 7 days, recording their death, as well as other manifestations of intoxication (behavioral change, temperature reactions, weight loss).

White mice of the control group were injected intraperitoneally with saline in a volume of 0.5 ml.

After 24 hours, and 7 days from the moment of introduction of the emulsion and the saline solution of sodium chloride of the animals from each group were euthanized ether anesthesia for evaluation of pathological and histological picture. With the same purpose were subjected to autopsy all fallen in the course of the trial animals.

During the observation period of individual animals comprising the experimental group to which the drug was administered in the maximum doses were recorded cases of refusal of food, reduction of motor activity, shiroshi is the use of wool. The results of the experiment are summarized in table 1.

The value of LD₅₀intraperitoneal injection was 0,562 ml, which exceeds the estimated therapeutic dose more than 20 times.

At the opening of the fallen during the experience of the animals observed phenomenon of redistribution of blood from her deposition in parenchymal organs of varying severity. The mucous membranes were hypermonogenic, with numerous punctate hemorrhages. There was no visible bleeding in the brain and internal organs. In cavities of a small amount of exudate mixed with blood elements.

The dissection of animals experimental and control groups, sleeping in the control period, did not reveal the pathology of internal organs.

For histological studies in animals were selected pieces of brain, kidney, adrenal, heart, liver and lung was fixed with 10%formaldehyde solution and embedded in paraffin. Sections were stained with hematoxylin-eosin. In the study of sectional material from dead animals registered numerous small hemorrhages in internal organs, disorganization CT is homicidal, dystrophic changes in cortical and cerebral layer of the adrenal glands. Signs of inflammation were not found.

To determine the chronic toxicity of ozonated emulsion was injected intraperitoneally two groups of mice in amounts of, respectively, one (0,02 ml) and two (0,04 ml) therapeutic doses taken in a volume of 0.5 ml of the Drug was injected five times with an interval between injections one day. Animals of the control group during the same period in the amount of 0.5 ml was injected intraperitoneally with saline.

Monitoring of laboratory animals was carried out during the entire period of injection, and within 7 days from the date of the last injection, by registering with possible manifestations of intoxication. After 24 hours, and 7 days from the date of the last data entry solutions of animals are put to sleep with chloroform to assess pathological picture.

During the observation period the animals ' death, as well as other pathological manifestations has not been registered. At necropsy, animals were observed uniformity of macroscopic picture: cavity without exudate, serous covers the regular color, internal organs without features, regular size, color and consistency. Signs of malnutrition, inflammation and hemodynamic disorders are not identified. Histological examination sectional mother who La also did not reveal pathological changes of internal organs.

For the evaluation of local action ozonated emulsion was administered to a group of white mice intramuscularly five times, with an interval of one day. The volume of emulsion for a single injection were 0.02 ml (one therapeutic dose). The control group of animals to the same extent and in the same time introduced a physiological solution of sodium chloride.

Observation of the animals was carried out during the whole period of the experiment, and within 7 days from the date of the last injection. On the availability of local damaging actions judged on the basis of data daily inspection of the place of introduction, and also by results of histological examination of the muscle tissue of animals euthanized with chloroform for 24 hours after the last injection and at the end of the observation period.

During the observation of signs of local inflammation and irritation of the drug was not detected.

The definition of immediate type hypersensitivity was performed by the method of playback of anaphylaxis in Guinea pigs. For this animal was previously senzibilizirani by a threefold introduction of ozonated emulsion with an interval between injections one day. First sensitizing injections were performed subcutaneously, two subsequent injections. The amount of emulsion to a single injection was 0.12 ml (estimated treatment e is for Guinea pigs). Two weeks after the final sensitizing injections the animals were injected intraperitoneally allowing the dose of the emulsion (0.25 ml), and then watched them for 30 minutes. The severity of the shock was evaluated according to the following scale:

A - theresiana wool, scratching paws muzzle.

B - periodic sneezing, lowering the temperature;

In - spastic cough syndrome, falling on his side;

Mr. sharp respiratory failure, convulsions, and death within 5...7 minutes;

E - there has been no reaction.

The index of the syndrome in the group was calculated by the formula Vaigla

Animals of the group of negative control instead of the pre-sensitizing injections according to the same scheme was injected with saline.

As a positive control animals were used, prepared for the shock sensitization of normal horse serum, introduced subcutaneously twice, in an amount of 0.1 ml per two weeks before the resolution intracardiac injection of this drug in a quantity of 0.2 ml of the results of the experiment are summarized in table 2.

Presented in table 2 data show that ozonated emulsion does not have anafilakticheskogo actions on Guinea pigs.

The delayed-type hypersensitivity characterized by change in mass of limbs animals.

This white mice were senzibilizirani intraperitoneally twice, with an interval of one day one therapeutic dose of ozonated emulsion (0,02 ml). Later, 5 days after the last sensitizing injections in the tip of one of the rear paws were administered 0.05 ml of ozonated emulsion, the other the same amount of saline. A day later, animals were euthanized with chloroform, the hind legs were amputated at the level of the heel joint and held their weigh-in. According to the difference of the mass of the legs were assessing the inflammatory reaction (edema). The swelling index was calculated by the formula

The mass of experienced paws;

VK - weight control paws.

As a control in this experiment, animals were used that instead of the pre-sensitizing injections in the same period and in the same volume was injected with saline. The results of the experiment are presented in table 3.

As seen from the digital data in table 3, the index of edema in both groups are almost identical, which suggests that ozonated emulsion does not cause hypersensitivity reactions of the delayed type.

Thus, in recommended doses ozonated emulsion is not toxic to animals, causing no symptoms of hypersensitivity reactions immediate and delayed type.

Conducted a study of antimicrobial activity of ozonized emulsion on various concentratie microorganisms (Staphylococcus aureus and Escherichia coli). Used 3 rows of sterile test tubes of 10 pieces, where one row served as a control, and two were experienced. In each tube was made with 4.5 ml of physiological solution. Then in the first test tube experienced a number of standard turbidity received the initial concentration of the investigated microorgani the MOU in 10 ⁹. Then, after thorough mixing transferred to the new measuring pipette 0.5 ml from one test tube to another, from the last test tubes 0.5 ml was poured. This has received a number of serial dilutions from 10⁹up to 10¹. In the 1st experimental series was added 0.5 ml of ozonated emulsion, in the 2nd experimental Newtonian emulsion, and in the control and 0.5 ml of physiological solution. The resulting mixture was left for 30 minutes at room temperature with constant shaking. After a specified time 0.1 ml of the contents of the tubes were sown on an appropriate dense nutrient medium and incubated in an incubator at 37°C for 72 hours. Every 24 hours by direct counting of colonies grown on solid nutrient medium from the experimental and control series, conducted an assessment of antimicrobial activity. These experiments are displayed in tables 4,5.

From analysis of digital material tables 4 and 5 we can conclude that ozonated emulsion with this mode of bubbling ozone-oxygen mixture has a pronounced anti-microbial properties in relation to the collection of strains as E. coli, and St. aureus. The distinguishing feature is the complete absence of growth of colonies in the experimental series compared to control.

Examined therapeutic efficacy of intrauterine injection of ozonated emulsion with postpartum acute purulent-naturalmommie in cows after normal calving. The results of the experiment are presented in table 6.

From digital material table 6 shows that the application of ozonized emulsion intrauterine cows, patients acute postpartum endometritis, 9.6% was more effective in comparison with 10%suspension of furazolidone. In addition, the CRA is of the introduction of ozonated emulsion on average 2.6 times was less in comparison with analog. Application of ozonized emulsion with the purpose of treatment led to higher rates of reproduction of the experimental cows was lower than 0.3, the rate of fertilization and 26.5 days shorter period of infertility).

In animals treated with ozonized emulsion, conducted a study of blood serum (table 7).

Materials table 7 show that the process of clinical recovery of the cows in the background of the application of ozonized emulsion was accompanied by activation of antitelomerase sequential synthesis of specific antibodies and the formation of circulating immune complexes to large size.

Conducted an experiment to examine the comparative efficacy of ozonated emulsion and drug Metrolex-M with acute postpartum endometritis, which is RA who was vivalda after surgical separation of placenta in cows (table 8).

From digital data in table 8 shows that the application of ozonized emulsion was 20% higher than using Metrolex) it Should also be noted that the ozonated emulsion causes a reduction in the duration of treatment and infertility animals, 0.2 reduces the rate of fertilization.

In cows treated with ozonized emulsion, the origin of which was ladowali serum (table 9).

Analysis of the results of the Hematology research (table) showed that in the course of the experiment, clinical recovery cows was accompanied by a decrease in the total protein due to active processes for the synthesis of specific antibodies involved in opsonization and elimination of antigens, as evidenced by high levels of circulating immune bodies of large size.

Therefore, the highest efficiency in the treatment of cows postpartum patients acute purulent-catarrhal endometritis, which occurs in cows after normal and abnormal labor, has intrauterine injection of ozonized emulsion.

Literature

1. Gavrish VG Intravenous method of treatment of endometritis in cows / Vggis, Yearjoin // Noncontagious Bo is esni animals: Mater, the Intern. scient. Conf., ]. 70 anniversary of Zooengineering faculty. - Kazan, 2000. - P.3-4.

2. Grigorieva I.E. the Treatment and prevention of endometritis in cows. M: Rosagropromizdat, 1988. - S. the prototype.

3. Patent of the Russian Federation No. 2311790. IPC A23D 9/04 (2006.01). The method of obtaining the ozonated emulsion / Platonov, VA, Klabukov ER, Konoplev I.G., Tregubov D.A.: applicants and patentees: GOU VPO "Kirov state medical Academy" of the Ministry of health and FSEI HPE "Vyatka state agricultural Academy Publ. 10.12. 2007. Bull. No. 34.

A method of treatment of endometritis in cows, including intrauterine introduction antimicrobial agent, characterized in that as an antimicrobial agent in intrauterine infuziruut to 100.0 ml ozonated emulsion consisting of equal parts of fish oil and extract of flax seed, every 48 h until clinical recovery.