Pectin- and chitosan-based biodegradable film |
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IPC classes for russian patent Pectin- and chitosan-based biodegradable film (RU 2458077):
 
Solid-phase method of producing bioactive nanocomposite / 2416389
Invention relates to synthetic polymer chemistry. The nanocomposite contains a matrix in form of a cross-linked salt of hyaluronic acid which is modified with sulphur-containing compounds and nanoparticles of a noble metal as filler. A film of the cross-linked salt of hyaluronic acid which is modified with sulphur-containing compounds is obtained through chemical reaction of the salt of hyaluronic acid with a mixture of two sulphur-containing compounds and with a cross-linking agent, under conditions with pressure between 50 and 300 MPa and shear deformation in a mechanical reactor at temperature between 20 and 30°C. The reactor used to obtain the film is a Bridgman anvil.
 Method for producing viable breast cells / 2409664
Solution of viable breast cells after enzymatic degradation in a collagenase solution of analysed tissue at temperature 37°C for 30-35 minutes, are applied on a biomaterial of a native form of hyaluronic acid, stored at room temperature and constant humidity 30-50 %, and cell viability is controlled by discoloration of cell monolayer.
 Novel cross-linking reagents for producing chitosan-based biocompatible materials / 2408618
Articles based on biologically active polymer materials can be used in surgery when treating wounds and as materials for temporary replacement of body tissue, in biotechnology for obtaining matrices for growing cell cultures, in pharmaceuticals as carriers of enzymes and other biologically active compounds. The cross-linking reagents are 2,4-derivatives of 3-oxa glutaric dialdehyde (2,2'-oxydiacetaldehyde). These compounds can be obtained via periodate oxidation of monosaccharides, nucleosides and nucleotides.
 Method of obtaining polysaccharide material / 2392972
Invention relates to a method of obtaining polysaccharide fibre for making materials, specifically for making surgical suture materials absorbable in a human and a mammal body, absorbable and non-absorbable dressing material and absorbable textile matrix materials. The method is characterised by that 2.4-4.0 wt % solution of polysaccharide in dimethylacetamide which contains 4.56-10.00 wt % lithium chloride is mixed with 1.0-5.0 wt % poly-N-vinylpyrrolidone with molecular weight of 8-35 kDa or a metallopolymer complex - fine-grained silver stabilised by poly-N-vinylpyrrolidone in such an amount that, content of fine-grained silver relative polysaccharide dissolved in spinning solution ranges from 0.07 to 0.87 wt %. Mass ratio of polysaccharide: metallopolymer complex equals 88.0-99.0:1.0-12 wt %. The mixture is intensely stirred, held, filtered, degassed and the obtained spinning solution is extruded at room temperature into an alcohol deposition tank in form of water-soluble aliphatic C2 and C3 alcohols. The fibre is then processed in plastification and washing tanks and dried.
 Taxanes covalently bonded with hyaluronic acid or hyaluronic acid derivatives / 2384593
Invention relates to taxane, especially to paclitaxel and docetaxel covalently bonded with hyaluronic acid or a hyaluronic acid derivative, used as active substance in pharmaceutical compositions which are used in oncology, for treating tumours, autoimmune diseases and restenosis, as well as a coating for stents and medical devices. The covalent bond is formed between hydroxyl groups of taxane and carboxyl groups or hydroxyl groups of hyaluronic acid or hyaluronic acid derivatives, or amino groups of deacetylated hyaluronic acid. Bonding can take place using a linking compound (spacer compound) which bonds taxane with hyaluronic acid or hyaluronic acid derivative under the condition that, the linking compound is different from hydrazide.
Composition - osteoinducer and osteoconductor in treatment of bone pathology in dentistry and oral surgery and method of obtaining composition - osteoinducer and osteoconductor in treatment of bone pathology in dentistry and oral surgery / 2383564
Composition includes chitosan gel, which has bactericidal properties, representing matrix for including in it of water solution for "Adgelon" injection, which contains serum glycoprotein from blood of livestock, possessing biological activity in supersmall doses 10-9-10-15 mg/ml, and calcium salt - preparation "Adgelon". In obtaining composition medicine "Adgelon" is immobilised on chitosan gel.
 Complex matrix for medico-biological application / 2360928
Complex matrix consists of at least one biologically compatible polymer of natural origin, structured with sewing agent, which represents two- or multi-functional molecule, selected from epoxides, epihalohydrines and divinyl sulphone, on said polymer inoculated are chains with molecular weight less than 50000 daltons, selected from polymers of natural origin of small size, preferably, derivatives of cellulose or other biological polymer derivatives which naturally are not present in human organism, and/or non-polymerised chains with properties of oxidation inhibitors or ability to inhibit reactions of matrix decomposition, preferably, vitamins, enzymes or molecules, consisting of one or several cycles, degree of inoculation, expressed as ratio of moles of inoculated molecules and quantity of moles of polymer units, constitutes from 10 to 40%. Also described are method of obtaining such matrix and its application for separation, replacement, filling or addition of biological fluid or tissues.
Method for production of modified paper / 2355842
Invention relates to papermaking technology, precisely to production of modified paper with higher gas-proof and heat-protective properties, and can be applied in constructions, aircraft and automobile constructions, shipbuilding. The method includes treatment of paper with the mixture of 5-7% aqueous solution of polyvinyl alcohol with 5-7% aqueous solution of chitosan at their ratio 1:1 within 10-15 minutes, thereafter treatment with 15-20% aqueous solution of methyl phosphate borate and drying.
Compound for production of modified paper / 2355841
Invention relates to production technology of synthetic paper, precisely to production of modified paper with higher gas-proof and heat-protective properties, and can be applied in constructions, aircraft and automobile constructions, shipbuilding. The compound contains 5-7% aqueous solution of polyvinyl alcohol, 15-20% aqueous solution of methyl phosphate borate and 5-7% aqueous solution of chitosan at the following ratio, pts. wt. polyvinyl alcohol - 5-7; chitosan - 5-7; methyl phosphate borate - 15-20; water - 275-266.
 Chitosanium perchlorate, method for its preparing and power-consuming composition comprising thereof / 2315774
Invention describes chitosanium perchlorate of the following formula: C6O4H9NH3ClO4. This compound shows such properties as explosion-proof, absence of toxicity, resistance to moisture and heating, mechanical effects and possesses high oxidizing and binding properties. This allows its using in power-consuming compositions in mixture with chitosanium dodecahydro-clozododecaborate wherein it functions as a binding agent. The quantitative ratio between chitosanium dodecahydro-clozododecaborate in the composition is determined by required regimen of combustion: the more content of chitosanium perchlorate the higher activity of the composition.
Method for production of pectin material / 2250229
Claimed method includes grinding and mixing of sunflower heads and citrus refuses in mass ratio from 1:9 to 9:1; hydrolysis-extraction of mixture; liquid phase separation and target product isolating. Before mixing starting materials are subsequently extracted with acetone and liquid acetylene. Hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage with acid additive are used, respectively.
Method for production of pectin material / 2250228
Claimed method includes grinding and mixing of sunflower heads and citrus refuses in mass ratio from 1:9 to 9:1; hydrolysis-extraction of mixture; liquid phase separation and target product isolating. Before mixing starting materials are subsequently extracted with dimethylformamide and mixture of liquid acetylene and carbon dioxide in ration from 3:7 to 7:3. Hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage with acid additive are used, respectively.
Method for production of pectin preparation from citrus refuses / 2250227
Claimed method includes hydrolysis-extraction and liquid phase separation wherein citrus refuses are forehead subsequently extracted with dimethyl formamide and mixture of liquid acetylene and carbon dioxide in mass ratio from 3:7 to 7:3. Hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage are used, respectively.
Method for production of pectin material from citrus refuses / 2250226
Claimed method includes hydrolysis-extraction and liquid phase separation wherein citrus refuses are forehead subsequently extracted with ethyl acetate and mixture of liquid acetylene and carbon dioxide in mass ratio from 3:7 to 7:3. Hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage are used, respectively.
Method for pectin production from citrus refuses / 2250225
Claimed method includes hydrolysis-extraction and liquid phase separation wherein citrus refuses are forehead subsequently extracted with acetone and liquid acetylene. Hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage are used, respectively.
Method for pectin production from citrus refuses / 2250224
Claimed method includes hydrolysis-extraction and liquid phase separation wherein citrus refuses are forehead subsequently extracted with acetone and liquid carbon dioxide Hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage are used, respectively.
Method for pectin production from citrus refuses / 2250223
Claimed method includes hydrolysis-extraction and liquid phase separation wherein citrus refuses are forehead subsequently extracted with acetone and mixture of liquid acetylene and carbon dioxide in mass ratio from 3:7 to 7:3. Hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage are used, respectively.
Method for production of pectin material / 2250222
Claimed method includes grinding and mixing of sunflower heads and citrus refuses in mass ratio from 1:9 to 9:1; hydrolysis-extraction of mixture; liquid phase separation and target product isolating. Before mixing starting materials are subsequently extracted with dimethylformamide and liquid acetylene under elevated pressure. When second extract is separated, pressure is discharged up to atmospheric one, and hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage with acid additive are used, respectively.
Method for production of pectin material / 2250220
Claimed method includes grinding and mixing of sunflower heads and citrus refuses in mass ratio from 1:9 to 9:1; hydrolysis-extraction of mixture; liquid phase separation and target product isolating. Before mixing starting materials are subsequently extracted with ethyl acetate and liquid acetylene under elevated pressure. When second extract is separated, pressure is discharged up to atmospheric one, and hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage with acid additive are used, respectively.
Method for production of pectin material / 2250219
Claimed method includes grinding and mixing of sunflower heads and citrus refuses in mass ratio from 1:9 to 9:1; hydrolysis-extraction of mixture; liquid phase separation and target product isolating. Before mixing starting materials are subsequently extracted with ethyl acetate and liquid carbon dioxide under elevated pressure. When second extract is separated, pressure is discharged up to atmospheric one, and hydrolysis-extraction is subsequently carried out in forward flow and backflow with intermediate pressing. As extractants in backflow process water and in forward flow process extract separated after backflow stage with acid additive are used, respectively.
 Polymer composition for moulding biodegradable articles from molten mass / 2446191
Invention relates to chemical and food industry, particularly to production of biodegradable plastic materials and can be used to make moulded or film articles for various purposes, including food purposes. The polymer composition for moulding biodegradable articles from molten mass contains polyolefins, biodegradable filler - starch and a process additive, which is a protein phosphatide concentrate (or fuzz) - a by-product of production of unrefined sunflower or rapeseed oil.
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FIELD: chemistry. SUBSTANCE: biodegradable film contains pectin, chitosan, water, 1N hydrochloric acid, a plasticiser - glycerine and a structure-forming agent - 3% methyl cellulose solution. EFFECT: obtaining a uniform biodegradable film without structural defects, which similar to household packaging polyethylene films on homogeneity, plasticity and strength. 2 dwg, 1 tbl
The invention relates to compositions of biodegradable films containing pectin for use in pharmaceuticals, medicine, veterinary medicine, food or cosmetic industry and for the manufacture of wrapping food film capsules. Known taken as the prototype of a highly elastic layered film made of chitosan and pectin. As the plasticizer can be added glycerine or lactic acid, and pectin or chitosan solution used for the manufacture of the film, may be added to the starch. The resulting layered film is biodegradable and made from renewable agricultural raw materials (U.S. patent No. 5919574). The disadvantage of the prototype is the considerable complexity of manufacturing the layered film, as it is necessary separately to produce a layer of pectin and chitosan layer film, in addition, the juxtaposition of these films to each other can be associated with the appearance of air bubbles between the layers, which is almost impossible to remove because the layers of the film sufficiently firmly stick together among themselves. Object of the invention is the simplification and acceleration of the process of making films of pectin and chitosan, obtaining biodegradable film with characteristics similar to plastic packaging films. Technical resultativity is to obtain homogeneous biodegradable films based on pectin and chitosan without flaws patterns, similar homogeneity, plasticity, strength for packaging plastic films domestic use. The technical result is achieved by the fact that in biodegradable film obtained from pectin, water, chitosan, odnomomentnoe hydrochloric acid and plasticizer according to the invention as a plasticizer use glycerin, and the film contains the amendment a three percent solution of methyl cellulose in the following ratio, wt.%:
The figure 1 shows pictures of biodegradable films based on pectin and chitosan according to the invention. The figure 2 shows photographs of the decomposition proposed film for 8 days. In the biodegradable film according to the invention, the optimum ratio of pectin and chitosan should be closer the ina 1:1. This ratio allows the films according to the invention to be strong (pectin gives the film studentsT), the lack of pectin structure of the film becomes too loose and liquid, the film may not harden. And when the excess pectin film becomes inflexible, at the same time the decomposition of the film increases, which is due to the greater density of the films. Chitosan is responsible for compatibility with animal tissue. Exposed to natural fungi, films containing chitosan, have a built-in source of nitrogen to increase biodegradation. I.e. the lack of chitosan significantly increases the period of decomposition films, reduced their degree of safety for the body. When excess chitosan film is opaque, will have a cloudy white color. Glycerin is responsible for the plasticity and flexibility of the films. When his lack of film is not stretched, bending cracks. With an excess of glycerol film is poorly separated from the surface becomes sticky, viscous. A three percent solution of methyl cellulose makes the film stronger. In excess of this component of the film becomes fragile. The lack - delicate, fragile. Biodegradable film according to the invention was prepared as follows. Pectin is dissolved in distilled water. Then Lu is the better dissolution of the components of the solution are placed in a thermostat for 1 minute at a temperature of 37-38°C. Chitosan was dissolved in 1 BC (odnomomentnoe) hydrochloric acid. For better dissolution of the components of the solution are placed in a thermostat for 1 minute at a temperature of 37-38°C. Then the obtained solutions of pectin and chitosan preferably in equal proportions poured and stirred until complete dissolution of the formed clots. In order for the film was solid and uniformly separated from the substrate after stirring clots in the resulting solution add the plasticizer and the amendment. The mixture is carefully poured into a Petri plate. Film formed on the glass substrate (in the Petri plate) for 20-24 hours at a temperature of from 0 to 25°C. The result is a homogeneous, transparent (depending on the thickness of the film is transparent or slightly turbid) film. The film is flexible, so that, when folded, does not form cracks during extension, the film takes its original state. The film has no colour, smell. It can be painted various dyes, such as food, since they are harmless to humans. The film may take various forms depending on the shape of the vessel into which is poured a solution: in the bowl Petri - round film (figure 1), in square container - square film. Change the color and shape of the films had no effect on their properties. The strength of biopolymer films was determined in accordance with Shasta 17035-86 using a universal testing machine with an Electromechanical drive (GOST 7855-84). Strength σr, MPa (H/mm2) was calculated by the formula:
where σr- tensile strength (MPa) N/mm2), Fr- tensile force (N), And0- the cross-sectional area (mm2). The strength of the obtained film are shown in table 1, where also the values of the indices of the films according to the invention in comparison with the similar indicators of polyethylene. As one of the main objectives of the present invention was to get a decent analog plastic packages, the most important properties of the resulting polymer films are shown in the table with the same properties of polyethylene films for domestic use (packaging). Based on these data we can conclude that the strength of polymer films is close to the strength of the polyethylene, but their safety and ability to undergo biodegradation expands the scope of their application. Table 1 shows the composition obtained according to the invention the film: its thickness is h, the length of the circumference is taken of the fragment - 1, the stretchability of the material in width in one direction - σwidththe stretchability of the material in the length in the other direction - σlength.
The film thickness was measured using a micrometer ELECTRO MS DIGITAL MICROMETER. She was 0,074 mm At different instances made film thickness ranged from 0,075 up to 1 mm. Evaluation of biodegradation of the films was carried out in the soil environment. Fresh film from a mixture of chitosan - pectin were fully biodegradable for 8 days (figure 2). After three-month storage film is decomposed, for 15 days, after half a year for 23 days. The invention is illustrated by example. 10 mg Apple Petina dissolved in 2 ml of distilled water. For a better solution was placed a solution in thermostat for 1 minute at a temperature of 37-38°C. 10 mg molefractions of chitosan from crustacean shell (manufacturer - JSC "Bioprogress") dissolved in 2 ml of 1 N. (odnomomentnoe) HCL. For a better solution was placed a solution in thermostat for 1 minute at a temperature of 37-38°C. Then merge the resulting solutions of pectin and chitosan. During draining of the solution of pectin with an equal volume of chitosan solution is the formation of clots at the contact interface of the two liquids. The clots have a gel-like structure. When defending going on the t slow deposition of clots at the bottom of the vessel without the formation of a homogeneous mass. Clots or removed from the solution, or stir until smooth. In order for the film was solid and uniformly separated from the substrate after stirring clots in the resulting solution add the plasticizer and the amendment. To impart plasticity add 1.5 ml of the plasticizer is glycerin. To give the film strength is added by amendment 2 ml of 3% solution of methylcellulose (3 g of methylcellulose + 100 ml water). Film formed on the glass substrate (in the Petri plate) for 20-24 hours at 20-25°C. After drying the film it is separated from the substrate. The film thickness was measured using a micrometer ELECTRO MS DIGITAL MICROMETER, she was 0,074 mm Biodegradable film obtained from pectin, water, chitosan, odnomomentnoe hydrochloric acid and plasticizer, characterized in that it contains as a plasticizer glycerin, as well as the amendment - a three percent solution of methyl cellulose in the following ratio, wt.%:
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