Method for electrochemical immunoassay for detecting viruses/viral antigens |
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IPC classes for russian patent Method for electrochemical immunoassay for detecting viruses/viral antigens (RU 2550955):
  
 Method of identifying catechol o-methyltransferase modulators / 2535125
Invention relates to medicine and describes method of identifying modulators of enzyme catechol-O-methyltransferase (COMT) activity, including a) obtaining 4-nitrocatechol, covalently bound with Alexa Fluor® 488, b) bringing molecule from stage a) in contact with enzyme catechol-O-methyltransferase (COMT), S-adenosylmethionine (SAM) and candidate compound and c) measurement of indices of fluorescence of mixture from stage b), in which changed indices of fluorescence in presence of candidate compound in comparison with the control serve as sign of presence of modulator of enzyme catechol-O-methyltransferase (COMT). Also described is method of identifying substrate of enzyme catechol-O-methyltransferase (COMT).
 Method for determining modified nucleotides of rna / 2522863
Selection of pair of oligonucleotide probes suitable for the study area of RNA, selection of a pair of donor and quencher of fluorescence with suitable optical properties is carried out. The direct chemical synthesis of modified probes is carried out, their effectiveness is assessed during melting with RNA containing known modifications. The obtained parameters of melting are assessed for each particular system. The control and the tested RNA are analysed, subjecting the mixture of probes and RNA to intense heating, slow cooling, and then the controlled heating with simultaneous detection of the fluorescence intensity, and the study parameters are selected according to the specific system. The results of melting are analysed, assessing the nature of the curves of melting, and the conclusion is made on the presence or absence of the modification in the test RNA in the presence or absence of modifications in the control RNA.
 Methods for determining efficacy of ligands of sodium/proton antiporters / 2519345
Invention refers to methods for determining the efficacy of an ion channel ligand. The ex vivo methods for determining the efficacy of the ion channel ligand in vivo depending on plasma, involves the stages as follows: a) contacting a cell expressing the ion channel with i) animal's plasma and ii) the ion channel ligand and b) determining the effect of the ion channel ligand on the cell or a) contacting the cell expressing the ion channel with i) animal's plasma and ii) a compound that is defined as the ion channel ligand, and b) determining the effect of the compound on the cell, or a) contacting the cell expressing the ion channel with animal's plasma wherein the ion channel ligand has been administered, and b) determining the effect of the ion channel ligand on the cell. The method according to the invention may be used for screening of a therapeutic preparation for preventing and/or treating a disease involving the ion channel dysfunction, especially for preventing and/or treating a cardiovascular disease or cancer.
 Ligands for aggregated molecules of tau-protein / 2518892
Invention relates to method of labelling paired helical filaments (PHF), which includes interaction of PHF with compound and detection of said compound presence, where compound has formula
 Detection method of proteins in amyloid state, and set for detection of proteins in amyloid state / 2509155
Invention proposes a detection method of proteins in amyloid state, in which a specimen of lysate of yeast culture or tissue of a mammal is obtained, ionic detergent is added to the specimen, proteins are concentrated in an amyloid shape on a cellulose acetate membrane, and they are detected by means of aptomeres, their conjugates or antibodies specific to amyloid shape of proteins. Besides, a set for detection of proteins in amyloid state is proposed.
 Optical visualisation agents / 2484111
Visualisation agent contains a conjugate of formula (I) of benzopyrylium dye through a linker group with a 3-100-dimensional synthetic peptide which provides directed delivery to the biological target. Also disclosed is a pharmaceutical composition which contains said conjugate of formula (I), a set for preparing said pharmaceutical composition and methods for visualisation of a mammal body in vivo.
 Fast biosensor with reagent layer / 2482495
Detection system for detecting target molecules includes a sensor chip (1), having on its detecting surface (33) an immobilised target molecule or a capturing molecule for target molecules and a soluble reagent layer (5), having a labelled molecule for binding with the target. The group of inventions also relates to a sensor chip (1) and a method of detecting target molecules in a sample using said sensor chip.
 Identification of molecules modulating protein-protein interaction / 2476891
Group of inventions refers to methods and systems of analysis based on enzymatic degradation following protein-protein interaction for reporter modulation (activation or inactivation).
 Method of cell population discrimination and application thereof / 2397494
There is offered a method of discrimination and calculation of at least two populations of biological elements - carriers of specific signs, probably presented in a sample. The method provides the use of three different probes, each of which is specifically fixed with one of the populations of biological elements which are required to be detected. Each probe itself becomes detectable due to its proper marker, and two different markers specified have two emission spectra containing at least one common part (overlapping emission spectra), and the third one has the emission spectrum which essentially contain no common parts with two others (nonoverlapping spectrum).
 Device and method for detecting flourescent marked biological components / 2390024
Device comprises a measuring cavity for receiving and introducing a fluid sample. The measuring cavity has a set fixed thickness not exceeding 170 micrometres. The measuring cavity has a section fit for acquisition of its image. Within the measuring cavity, there is a dry reagent. The reagent contains as a component, a molecule conjugate with phosphor used for binding with biological components and with all other reacting components. The reacting components are soluble and/or suspended in the fluid sample. The method involves mixing of the reagent with the liquid sample to be introduced in the measuring cavity. A section of the sample in the measuring cavity is exposed to electromagnetic radiation of wavelength corresponding to wavelength of phosphor excitation. Phosphor marked biological components are detected through-thickness of the measuring cavity. Further, numerical analysis of the digital image follows to identify the biological components showing phosphor and to determine amounts of the biological components showing phosphor in the sample. The biological components are discernible on the digital image as fluorescing points emitting electromagnetic radiation of wavelength corresponding wavelength of phosphor emission.
Method of predicting development of critical stenosis of coronary arteries in patients with ischemic heart disease / 2550947
In the patient's blood serum the concentration of matrix metalloproteinase - 9 (MMP-9) and/or procollagen 1 C-terminal propeptide (PICP) is determined. If the value MMP-9 is larger than 101.8 ng/ml and/or PICP 195.6 ng/ml the unfavourable course of IHD is predicted, if lower than these values - the favourable course without the formation of critical stenoses in the coronary arteries.
 Method of determining functional activity of human complement factor b / 2550946
Invention relates to medicine, namely to immunology, and describes a method of determining the functional activity of the human complement factor B by its influence on target cells in a calcium-free medium, which contains magnesium ions, in the presence of the reagent RB, representing human blood serum, selectively deprived of the factor B activity, as the target cells selected are infusoria Tetrahymena pyriformis, the suspension of which together with the tested sample, which contains the determined factor B, and the reagent RB is introduced in measuring cells of the device for the automated calculation of the number of alive infusoria with the following determination of the number of alive cells in each minute, and the concurrence of dynamics of the change in the number of alive cells in time for the tested sample and control one, which represents a pool of 10 serums of healthy donors, supposes the equality of activities of the complement factor B in the said samples.
 Method for individual selection of immunocorrective therapy for prevention of frequent respiratory infections in primary school-aged children / 2550921
Children are divided into 4 groups; the first group includes the children having the following symptoms: short period of breastfeeding in the medical history, frequently ill family members, no complications of respiratory infections, IgA from 0.7 to 1.1 mg/ml, IgG from 10.6 to 16.2 mg/ml; the second group: mother's gestosis during the pregnancy, threatening miscarriage in the medical history, frequent complications of respiratory diseases - sinusitis, otitis; alpha2-globulin from 9.4 to 11.8 g/l, gamma-globulin from 17.8 to 22.6 g/l, TNF-α from 8.7 to 10.1 pg/ml, IgG from 16.7 to 23.7 mg/ml, CD3+ from 51 to 56.2%, CD4+ from 27.2 to 29.4%, CD16+CD56+ from 6.5 to 8.5%, nitro blue tetrazolium reduction test from 25 to 32.8%; the third group: mother's anaemia during the pregnancy, child's positive endocrine heredity, respiratory diseases frequently complicated with bronchitis, Candida seeding in throat swabs, gamma globulins from 12.1 to 14.9 g/l, IgG from 10.9 to 15.5 mg/ml, CD19+ from 7.6 to 9.6%, nitro blue tetrazolium reduction test from 25.5 to 31.7%; the fourth group: allergic reactions in the child's medical history, positive allergic heredity, respiratory diseases frequently complicated with obstructive bronchitis, gamma globulins from 18.1 to 23.1 g/l, IgE from 67 to 261 IU/ml. Acute respiratory diseases are prevented by Multitabs, a vitamin mineral complex, and one of the immunocorrective preparations: in the first group with IRS-19, a preparation of bacterial lysates; in the second group with a preparation of glucosaminyl muramyl dipeptide (Licopid); in the third group with a preparation of azoximer bromide (polyoxidonium); in the fourth group the immunocorrection is not recommended.
Method for predicting hospital-acquired intestinal infection in infant / 2549982
Invention refers to medicine, namely to paediatrics, and can be used to predict hospital-acquired intestinal infection in an infant. That is ensured by quantitative measurement of the sIgA content in coprofiltrate at admission to hospital. If the sIgA level is less than 23.2-63.5 mg/l, a secondary hospital-acquired intestinal infection is predicted.
 Method of preserving immunoperoxidase conjugate / 2549971
Invention consists in providing a method of preserving an immunoperoxidase conjugate using protein stabiliser solution based on an aqueous emulsion of hen egg white as a stabilising medium of drying while lyophilisation and after dissolving the product with preserving the stability of physical and immunochemical indicators.
 Binding proteins specific to insulin-like growth factors, and using them / 2549703
Present invention refers to immunology. What is presented is a completely human monoclonal antibody, which binds insulin-like growth factor-II (IGF-II) and has a cross responsiveness to IGF-I, as well as its antigen-binding fragment. There are disclosed a nucleic acid molecule coding an antibody according to the invention, a vector and a host cell for the expression of the antibody according the invention. There are described a pharmaceutical composition, as well as conjugates for treating and diagnosing malignant tumour, using the antibody according to the invention in preparing the therapeutic agent and a method for determining IGF-II and IGF-I levels in a patient's sample.
 Borrelia hybrid protein, nucleic acid coding this protein, expressing cartridge, vector, method and kit for diagnosing lyme borreliosis, vaccine for borreliosis prevention / 2549698
Presented group of inventions concerns fused proteins, nucleic acids coding these proteins, an expressing cartridge providing nucleic acid expression, a vector comprising this cartridge, a diagnostic technique for in vitro borreliosis, a kit for this diagnostic technique, which use these proteins, as well as a vaccine composition for preventing borreliosis containing these proteins. The characterised fused proteins contain (i) at least one sequence of DbpA protein of the species Borrelia specified in B. afzelii, B. burgdorferi sensu stricto and B. garinii, and (ii) least one sequence of OspC protein of the species Borrelia specified in B. afzelii, B. burgdorferi sensu stricto and B. garinii.
 Pcr method, applying polymer material as carrier / 2549694
Group of inventions relates to the field of biochemistry. Claimed is a device for nucleic acid sampling, application of the device of nucleic acid sampling, a set for nucleic acid amplification, as well as a method of nucleic acid amplification. The device includes a probe for holding the nucleic acid sample and a manipulator, connected to the probe with an account of a possibility of the probe manoeuvring. The probe includes a multitude of probe elements, which are capable of travelling with respect to each other between approximated and mutually remote configurations. Each probe element has a nozzle. When the probe elements move into an approximated configuration, each nozzle is joined with other nozzles to form a composite nozzle. The composite nozzle is capable of contacting with nucleic acid in order to deliver its sample. The method of amplification of nucleic acid from higher eukaryotes includes the contact of the sampling device with a source of nucleic acid from higher eukaryotes, introduction of the sampling device or its part into a reaction vessel to carry out the reaction of nucleic acid amplification without preliminary material processing and realisation of the reaction of nucleic acid amplification.
 Specific binding proteins and applications thereof / 2549678
Present invention refers to immunology. Presented is an antibody able to bind to an amplified epidermal growth factor receptor (EGFR) and to de2-7 EGFR, a truncated version of EGFR, and characterised by sequences of variable domains. There are also disclosed a kit for diagnosing a tumour, an immunoconjugate, pharmaceutical compositions and methods of treating a malignant tumour based on using the antibody according to the invention, as well as a single-cell host to form the antibody according to the present invention.
 Diagnosing chronic obstructive pulmonary disease (copd) / 2549481
Group of inventions refers to medicine. Presented are diagnostic technique for chronic obstructive pulmonary disease (COPD) and differentiating stages I/II and III/IV COPD in a human involving measuring heat-shock protein 27 (HSP27), 70 (HSP70) and 90 alpha (HSP90 alpha) and measuring interleukin-1 (ST2) receptor 4 or histon-related DNA fragments in a sample. The COBD is diagnosed, when the measured amount of HSP27, HSP70, HSP90 alpha, ST2 and histon-related DNA fragments appears to increase as compared to that in the healthy people. The stage I/II COBD is diagnosed, when the measured amount of HSP27 in the sample of the above individual falls within 2,600 and 3,300 pg/ml, the amount of ST2 is more than 160 pg/ml, while the amount of HSP70 and HSP90 alpha is at least 40% increased as compared to that in the healthy people. The stage III/IV COBD is diagnosed, when the amount of HSP27 in the sample falls within 3,400 and 5,500 pg/ml, while the amount of HSP70, HSP90 alpha and histon-related DNA fragments is at least 40% increased as compared to that in the healthy people.
 Method for predicting the character of bacterial keratitis flow / 2245553
In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula:
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FIELD: medicine. SUBSTANCE: invention involves the sandwich-type immune complex formation between antibodies and measles virus/viral antigen followed by attaching an antibody conjugate with a signal-forming label; the signal-forming label represents magnetic nanocomposite particles that are produced by the silicon oxide coating formation on the surface of the magnetic nanoparticles of transition metal oxide, which is supposed to be conjugated with the antibodies; before the stage of the immune complex formation. The immune complex is formed by concentrating it on a solid-phase chemically inert carrier as a result of the magnetic field exposure, which is followed by removing this immune complex from a medium formed on a medium formed on the carrier; the virus/viral antigens and their concentration are detected and measured by a signal generated by transient metal ions formed by the acid treatment of the immune complex. EFFECT: simplifying the analytical procedure, increasing the rate and reproducibility. 3 cl, 4 dwg, 3 ex
The invention relates to biologists and medicine, namely to the immunoassay, in particular to electrochemical methods for determination of virus/virus antigens in various objects and can be used for differential diagnosis of infectious diseases. The disadvantages of currently used methods of immunoassay are: the need to create special conditions for their implementation, such as methods of analysis based on polymerase chain reaction, and the high cost of the reagents used and the equipment, for example enzyme-linked immunosorbent assay. The prior art discloses a method mnogoukladnogo immunoassay with the use of microparticles, comprising mixing of different categories of microparticles coated biospecifically reagents for binding a variety of desired analytes and labeled with one or more fluorochromes at different concentrations, emitting long-lived fluorescence, the addition to the mixture of microparticles of the test sample and biospecifics developing reagent labeled detection fluorochrome, conducting the reaction for the formation of biospecifically complexes, the excitation of the fluorochromes and the dimension in the temporary authorization amount of long-lived fluorescence emission to identify the category� of microparticles and the amount of fluorescence emission detector fluorochrome for measuring the amount of desired analytes, thus formed biospecifically complexes carbide is precipitated on a carrier, the emission fluorescence of all fluorochromes excited by sources of radiation in two spectral ranges, for tagging biospecifics showing the detection reagent is used fluorochrome with the short-lived fluorescence, the area of excitation and emission of fluorescence which is outside the regions of excitation and emission fluorescence of fluorochromes with long-lived fluorescence, the ratio of the concentrations of fluorochromes with long-lived fluorescence of the microparticles to determine one type of analyte constantly, and to identify different types of analytes the concentration ratio varies at least twice (see RF patent №2379691 for the invention "Method mnogoukladnogo immunoassay with the use of microparticles", priority date 07.07.2008 G., published 20.01.2010) The disadvantages of this method are due to the need for a multi-stage process, and the difficulty of obtaining an analytical signal. The known method mnogoukladnogo immunoassay with the use of microparticles, comprising coating the surface of a porous membrane of the reaction mixture containing the analyte, the first binding molecule associated with the detecting substance and specific to the analyte, explore�th sample and the particles, not able to pass through the membrane pores, coated with the second binding molecules, also specific for the analyte, incubating the mixture for the formation of biospecific complex, the laundering of the reaction mixture from unbound reagents and the detection of the analyte in the sample according to the light signal of the detecting substance associated with biospecifically complex, thus use at least two types specific to the sought analytes of the first and second binding molecules, wherein each species of molecules is specific to only one desired analyte, each of the first binding molecule is associated with at least two long luminescent detection substances the ratio of the concentrations in each of the first binding molecule selected in advance and corresponds to a certain desired analyte is recorded in the temporary permission phosphorescence signals in the spectral ranges of emission detection of substances corresponding to the time constant of decay of these substances, and determine the desired analyte by the ratio of the phosphorescence signal, wherein the spatial resolution of the detection system, Δ (µ2) is determined from the following relationship: Δ≤S/10Nµ2where S - the area of adsorption of particles on the membrane, µ2; N is a specific number of latex� particles in the sample (see RF patent №2339953 for the invention "Method mnogoukladnogo immunoassay with the use of microparticles", the filing date 13.06.2007 G., published 27.11.2008 g). This method allows you to detect and quantitatively detect low concentrations of biological analytes in samples in research on a solid surface. The need for the use of the membrane makes high demands on the composition of the sample. The disadvantages of this method are the need for a multi-stage process, and the difficulty of obtaining an analytical signal. The closest technical solution to the claimed invention, selected as a prototype, is a method, carried out using electrochemical immunosensors developed for the detection of surface antigen of hepatitis B. First, biotinylated antibodies to hepatitis b immobilizers coated with streptavidin on the magnetic nanoparticles. Then add the antigen of hepatitis B virus and after this phase is injected secondary antibodies conjugated to signalosome label, which is used as a horseradish peroxidase. The result is the formation of immunocomplex on a "sandwich". Thereafter, a working substrate, which is used as the buffer solution of Britton-Robinson containing re�ord hydrogen and antoniniana, the horseradish peroxidase catalyzes the oxidation of aminophenol 3-aminophenazone. Then take an aliquot of the sample, transfer it to the electrochemical cell and register a cyclic voltamperometry. The magnitude of the peak current of oxidation is proportional to the number 3 aminophenazone, which, in turn, is proportional to the content labeled with horseradish peroxidase "sandwich"structures, and hence the content of the antigen of hepatitis b virus. This method of immunoassay allows you to define from 0.001 to 0.015 ng/ml of antigen, the detection limit of 0.9 ág/ml at a signal/noise 1/3 (see Magnetic nanoparticle-based immunosensor for electrochemical detection of hepatitis surface antigen In / Sara Nourani, Hedayatollah Ghourchian, Seyed Mehdi Boutorabi // Analytical Biochemistry, 2013, V. 441, P. 1-7). The disadvantages of this method include a multistage process analysis (pre biotinylating antibodies, conjugation of antibodies with horseradish peroxidase), high labor-intensive, time-consuming and also high requirements to qualification statements. The technical result, which is aimed to offer the protection of the technical solution is to simplify the analysis process, the increase of quick testing were and reproducibility. The specified technical result is achieved in that the proposed method of electrochemical immunogen�Lisa to identify viruses/antigens of viruses, comprising the formation of immunocomplex on a "sandwich" between the antibodies and the virus/virus antigen followed by the addition of conjugate antibodies with signalosome label for the purpose of determining the presence and concentration of virus/virus antigen by obtaining electrochemical response due to the use of signalosome label, which is part of the "sandwich"structure, according to the invention as signalosome label use of magnetic nanocomposite particles, which before the stage of formation of immunocomplex get by creating on the surface of magnetic nanoparticles transition metal oxide oxydrene coating followed by obtaining the conjugate with antibodies, in this case the formation of immunocomplex (antibody - virus/virus antigen - antibody with signalosome magnetic label) is carried out by its concentration on the solid inert carrier by exposure to a magnetic field with the subsequent seizure of immunocomplex from the environment, formed on the carrier, and detecting the presence and concentration of virus/virus antigen is performed on a signal generated by transition metal ions resulting from the acid treatment of immunocomplex, wherein the transition metal is used in iron, and as terdapat�wow chemically inert carrier - thick graphite electrode. To ensure interaction between the magnetic nanoparticles and antibodies to magnetic nanoparticles can be modified by aminopropyltriethoxysilane, forming, thereby, execrative coverage. For acid treatment, contributing to the chemical destruction of immunocomplex, use a mixture of nitric and sulfuric acids. The formation of immunocomplex significantly reduced through the use of magnetic concentration of immunocomplex on a solid phase carrier, which, in turn, allows you to accelerate the procedure for immunoassay. In addition, increases sensitivity. The concentration of virus/virus antigen is determined by obtaining the electrochemical response recorded from ion Fe3+obtained by acid treatment of the carbon substrate with immobilizerturning the immunological complex. Technical solutions, which coincides with the set of essential features of the claimed invention, not identified that allows to make a conclusion on compliance of the claimed invention to such a condition of patentability as "novelty". Declare the essential features that determine the receipt of the indicated technical result that is explicitly not follow from the prior art, which allows to conclude that according�accordance of the present invention, such a condition of patentability, as "inventive step". The condition of patentability "industrial applicability" is confirmed on the example of a specific implementation. The claimed invention is illustrated by drawings, where Fig.1 (a) front view carbon substrates - thick graphite electrode (CGE); b) is a side view of a carbon substrate - thick graphite electrode (CGE); Fig.2 - cyclic voltamperometry registered in model solutions containing (a, 4-6) and not containing (b, 4-6) antigen of measles virus (NovO/96), where 4 - voltamperometry of the supporting electrolyte, 5 - voltamperometry the model solution, 6 - voltamperometry registered after the introduction the model solution of the additive ions Fe3+; Fig.3 - cyclic voltamperometry registered in samples containing (a, 7-9), and not containing (b, 7-9) the measles virus, where 7 - voltamperometry of the supporting electrolyte, 8 - voltamperometry samples, 9 - voltamperometry registered after the introduction of additives to the sample ions Fe3+. Fig.4 - cyclic voltamperometry registered in samples containing (a, 10-12), and not containing (b, 10-12) the measles virus, where 10 - voltamperometry of the supporting electrolyte, 11 - voltamperometry samples, 12 - voltamperometry registered after the introduction of additives to the sample ions Fe . Thick graphite electrode - tungsten carbide carrier consists of a carbon substrate 1 (electrode) made of fiberglass, which contained a track of conductive material 2, which is used, for example, graphite composition, carbon ink. Track 2 is coated with the layer of the insulator 3 or cementite. The claimed invention is confirmed by examples of specific performance. Example 1. The model solution with the antigen of measles virus (NovO/96) were incubated for 30 minutes at 37°C with magnetic nano-particles of Fe3O4-SiO2on which surface immobilizerpower polyclonal IgG to measles virus. After incubation the solution is placed the (Fig.1), a modified IgG, and incubated for 20 minutes at 37°C. To accelerate the delivery to the surface of the nanocomposite particles with immobilizerturning on the surface of the immunological complex using a magnetic field. Then the electrode was washed with buffer containing normal horse serum (TLS) and tween 20. Extracted from the analyzed solution, the electrode is placed in a mixture of nitric and sulfuric acids (0.36 M H2SO4and 0.28 M HNO3for the subsequent destruction of the "sandwich" and the dissolution of the nanocomposite particles. As a signal characterizing the content�of virus/virus antigens in the sample using electrochemical response of iron passed into the acid solution at the destruction of the "sandwich." To conduct no-load experience using a phosphate buffer not containing antigen of the virus (NovO/96) (Fig.2). In the model solution discovered 8×10-2mg/ml of antigen. Example 2. The sample containing the washings from the nasopharynx of a patient infected with measles virus, incubated for 30 minutes at 37°C with magnetic nano-particles of Fe3SO4-SiO2on which surface immobilizerpower polyclonal IgG to measles virus. After incubation the solution is placed the (Fig.1) a modified IgG, and incubated for 20 minutes at 37°C. To accelerate the delivery to the surface of the nanocomposite particles with immobilizerturning on the surface of the immunological complex using a magnetic field. Then the electrode was washed with buffer containing normal horse serum (TLS) and tween 20. Extracted from the analyzed solution, the electrode is placed in a mixture of nitric and sulfuric acids (0.36 M H2SO4and 0.28 M HNO3) for the destruction of "sandwich" and the dissolution of the nanocomposite particles. As the signal characterizing viruses/virus antigens in a sample, using electrochemical response of iron passed into solution by acid destruction "se�of dicha". To conduct no-load experience using a sample containing the washings from the nasopharynx of a healthy patient (Fig.3). In a sample taken from an infected patient, detected concentrations of virus, corresponding to 3×10-3mg/ml of antigen. Example 3. The sample containing the washings from the nasopharynx of a patient infected with measles virus, incubated for 30 minutes at 37°C with magnetic nano-particles of Fe3O4-SiO2on which surface immobilizerpower polyclonal IgG to measles virus. After incubation the solution is placed the (Fig.1) a modified IgG, and incubated for 20 minutes at 37°C. To accelerate the delivery to the surface of the nanocomposite particles with immobilizerturning on the surface of the immunological complex using a magnetic field. Then the electrode was washed with buffer containing normal horse serum (TLS) and tween 20. Extracted from the analyzed solution, the electrode is placed in a mixture of nitric and sulfuric acids (0.36 M H2SO4and 0.28 M HNO3) for the destruction of "sandwich" and the dissolution of the nanocomposite particles. As the signal characterizing viruses/virus antigens in a sample, using electrochemical response of iron passed into the acid solution at the destruction of the "sandwich." For a blank experience used�isout sample containing the washings from the nasopharynx of a healthy patient (Fig.4). In a sample taken from an infected patient, detected concentrations of virus, corresponding to 4×10-4mg/ml of antigen. The proposed method can significantly reduce material and labor costs required for carrying out the process, increase productivity and reduce cost definition. In addition, the invention allows to simplify the process of immunoassay, as well as to increase the rapidity and reproducibility, and use it in different environments. 1. Method of electrochemical immunoassay for determination of viruses/antigens of measles, including education immunocomplex on a "sandwich" between the antibodies and the virus/virus antigen followed by the addition of conjugate antibodies with signalosome label for the purpose of determining the presence and concentration of virus/virus antigen by obtaining electrochemical response due to the use of signalosome label, which is part of the "sandwich"structure, characterized in that as signalosome label use of magnetic nanocomposite particles, which before the stage of formation of immunocomplex get by creating on the surface of magnetic nanoparticles transition metal oxide oxydrene coating with subsequent proceneonate with antibodies, in this case the formation of immunocomplex (antibody-virus/virus antigen - antibody with signalosome magnetic label) is carried out by its concentration on the solid inert carrier by exposure to a magnetic field with the subsequent seizure of immunocomplex from the environment, formed on the carrier, and detecting the presence and concentration of virus/virus antigen is performed on a signal generated by transition metal ions resulting from the acid treatment of immunocomplex, wherein the transition metal is used in iron, and as the solid chemically inert carrier - thick graphite electrode. 2. Method of electrochemical immunoassay for determination of viruses/antigens of measles virus according to claim 1, characterized in that to enable interaction between the magnetic nanoparticles and antibodies to magnetic particles coated with aminopropyltriethoxysilane. 3. Method of electrochemical immunoassay for determination of viruses/antigens of measles virus according to claim 1, characterized in that the acid treatment, a mixture of nitric and sulfuric acids.
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