Method for stimulating metabolic processes in biological tissues experimentally

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to stimulating metabolic processes experimentally. The patient is exposed to a pulse magnetic field. Thereafter, an inductor coil is applied with its north pole placed on the regions with maximum prominent trophic involvements, a projection of a distal aorta, iliac and femoral arteries. The exposure represents three 7-day courses daily every 5 days. On the first two days of the first course, the exposure length is 7 minutes, the frequency is 5 Hz, the voltage is 600 V, and also for 3 minutes at the frequency of 1 Hz, the voltage of 600 Hz. On the following two days, the voltage increases to 650 V, the exposure length increases by 1 minute at both frequencies. On the following three days of the first course, the voltage increases to 700 V, the exposure length increases by 1 minute at both frequencies. The second course is characterised by the voltage of 700 V, the exposure length increased at both frequencies on the third, fifth, and seventh sessions. The third course is performed daily at the voltage of 700 V at the maximum exposure length of 10 minutes at the frequency of 5 Hz, and of 6 minutes at the frequency of 1 Hz.

EFFECT: method enables repairing the microcirculatory vessels.

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The present invention relates to the field of medicine, namely physiotherapy, in particular to magnetic therapy, and can be used for positive therapeutic effects on biological objects, in particular in the treatment of inflammatory and postoperative wounds, but also to stimulate metabolic processes in the acceleration of wound healing and damage of various etiologies low reparative abilities of tissues.

Currently, the most significant problem, causing disability in the working population, is atherosclerotic arterial lesions of the heart, brain and lower extremities. So, in Russia every year coronary heart disease (CHD) ill 8 young people (aged 20 to 24 years) per 1 million population. In the age groups 60 to 64 years figures annual incidence of CHD increased to 1712 people per 1 million inhabitants. Analyzing the frequency of occurrence of ischemic stroke, it should be noted that in Europe the frequency of occurrence of this disease is about 2500 cases per 1 million people per year and, thus, takes on the character of "epidemic". The prevalence of atherosclerotic lesions of the cardiovascular system (CVS) force many large research centers to study this disease is.

According to modern concepts, atherosclerosis due to occlusive-stenotic lesions of the trunk and peripheral arterial bed in krovosnabjaemah organs and tissues there is a decrease or cessation of arterial blood flow, which contributes to tissue hypoxia and disruption of tissue metabolism (ischemia tissue).

The combination of atherosclerosis and diabetes are created favorable conditions for the development of lesions of the vascular bed, which leads to blood circulation not only in the main blood vessels, but also in microvasculature (arterioles, capillaries) - there are macro - and microangiopathy. It is the defeat of the microvasculature (microangiopathy, capillarity) with atherosclerosis and diabetes associated with narrowing and obliteration of lumen arterioles and capillaries, negates all the efforts of vascular surgeons and therapists.

Modern methods of treatment in atherosclerotic lesions of arteries are divided mainly into two groups: surgical, medical and complex, including and physiotherapy.

Surgical methods deobliteration lumen arterial include balloon dilatation, stenting, endarterectomy, bypass surgery, prosthetics. Surgical treatment is invasi the tion, expensive and applicable in the localization process in vessels with a diameter > 2 mm, which does not allow treatment of diseases of the distal arteries, often also affected (Savage M.P., Goldberg, S., A.A. Bove et al. Effect of thromboxane A2 blokade on clinical outcome and restenosis after successful coronary angioplasty. Multi-Hospital Eastern Atlantic Restenosis Trial (M-HEART II). Circulation 1995; 92:3194-3200).

Modern therapeutic drugs (angioprotectors, anticoagulants, disaggregants, statins and vasoprotective) only stabilize the clinical picture, enhancing the adaptive mechanisms of tissues and organs to ischemic changes. Therapeutic treatment is expensive, possible adverse reactions (allergic reactions, "syndrome victimize", disorders of the blood coagulation system), you must also consider the compatibility of drugs, comorbidity (Ushkalov E.A. Adding clopidogrel to aspirin may be useful for patients with symptomatic atherothrombosis, but is not recommended for patients with multiple risk factors: results CHARISMA; Farmateka, cardiology neurology, 13 (128), p.16-18, 2006).

The main disadvantage of the above methods, both surgical and pharmacological is their lack of effectiveness is the lack of meaningful positive impact on the affected microcirculation.

Known method of promoting p is acesso activity of biological objects, including impacts on biological object electromagnetic field (RF patent No. 2113108, IPC A61N 2/00, publ. in BI from 20.06.1998 year).

The known method is as follows

Stimulation of the processes of vital activity of biological objects is exposed to an electromagnetic field by means of two electrodes. The electrodes serves voltage, for example, from the network via a step-down transformer with adjustable in the range of 3-12 Century Closing the electrodes to each other, regulate the current in the circuit within 1-400 mA. This is preparation for the stimulation process. Then the electrodes are connected to the bio-object, pass an electric current and is affected by the magnetic field through a coil included in the circuit of the electrodes and placed on one of them. The magnitude of the magnetic field set in the range 1-1000 E.

However, the known method is intended for exposure (according to the presented examples of specific performance) in animals and plants.

A prototype of the invention is selected known method of stimulation of metabolic processes in tissues, including the effects of shock wave pulses on the areas most pronounced trophic characteristics (RF patent No. 2076641, IPC AV 17/233, publ. 10.04.1997,).

The known method is as follows. Directly settlement is e conducted operations in the experiment formed a skin wound is exposed to stimulating shock wave pulses of microsecond range with amplitude compression 3-20 MPa. The angle of the front of the pulse is chosen in the range 15-60° relative to the time axis in the coordinates of the pressure - time when politicaleconomy division sweep measurement device. Supply shock-wave pulses is carried out with the pulse width at its half-height of 0.6 to 2.0 μs, the greater the angle of the front of the shock wave pulse corresponds to the upper limit of the amplitude ratio, and the smaller the amplitude corresponds to a larger value of the duration of the shock wave pulse. Technical result: the achievement of active regeneration of healing of skin wounds and active stimulation of arterial and capillary blood flow.

According to the prototype method, the most efficient processes stimulation occur when the load is close to bell-shaped pulses, and the amplitude compression (i.e. positive phase pulse) less than 20 MPa. The inclination of the focus of the shock wave pulse in a known way vary from 15 to 60° (relative to the time axis on politicaleconomy the scan pulse in the coordinates of the amplitude-time), and the lower limit of the amplitude compression of the shock wave pulse is created by not less than 3 MPa, since the tilt of the front less than 15° (politicaleconomy scan) stimulation process is waning fast because it exceeds the characteristic size of the wavelength of the pulse, the characteristic p is Smurov stimulation region in the area of interest and, besides, there is just not sufficient amplitude to activate processes at the cellular level. When you angle greater than 60°, the shock wave pulse already begin to show characteristics with damaging factor, because when you use shock wave pulses for crushing concrements the angle of the front to the time axis order 70-87°.

In the method prototype supply shock wave pulses is carried out with the pulse duration at half-height of its amplitude from 0.6 to 2.0 µs, according to the authors, at lower durations angle of the slope (steepness of the front of the pulse exceeds 60° (politicaleconomy the scan) and the dynamics of the perturbations may contribute to the destruction of tissue, and, on the other hand, at duration, large 2,0 ISS, the lifetime of the perturbation can lead to the destruction of tissue due to "too long" (relative to the time of natural drainage of excess energy from the point of its absorption to the adjacent tissues the bio-object) exposure time of disturbance. That is why the shock wave load pulse is supplied.

Stimulating shock wave pulses in the method prototype in front steepness and amplitude do not have the characteristics of shock-wave pulses for lithotripsy. Shock wave beam initiate the reflector electrohydraulic way and down the via matching medium (water) to the surface of the biological object to eliminate reflections of the beam from the skin of the bio-object. Then set the amplitude of the pulse compression in the range from 3 to 20 MPa by changing the charge capacity of the generator pulses, the charging voltage and interelectrode distance in an underwater spark gap, i.e. known trick perform actions for the implementation of the parameters of this method and conduct a series of pulses (for example, from 500 to 3,000) per session.

As the sessions noted the rapid development of granulation tissue, reducing the size of the wound and its full epithelization on the 13th day. Histological examination longer healed wounds noted the proliferation of Mature connective tissue in the skin and minor areas of subcutaneous fat. In a proper plate of the epidermis and the subcutaneous tissue is noted an abundance of blood vessels of the microvasculature, simpaticos and isolated nerve endings.

The disadvantages of this method are as follows.

For expansionary effects (stimulation of metabolic processes in tissues and organs, accompanied by the growth of blood vessels of the microvasculature and the stimulation of healing of trophic ulcers) requires mandatory application of the matching environment (water)located on the surface of the biological object to eliminate reflections of the beam from the skin of the bio-object. Use water IP environment which incorporates both the ability to provide a stimulating influence on the biological object, not directly in contact with the reflector (emitter). Example: it is impossible to make an impact on the organs and tissues, closed clothes must be in contact with the skin.

For expansionary impact must take into account the fact that the passage of the shock wave to the lesion through the bone structure can lead to significant pain. In addition, the shock wave weakens when passing through the bones and organs with high lightness (cavity with air, light)that, in some cases, leads to inefficiency effects.

The known method is difficult to apply in clinical practice in patients with dysfunction of the musculoskeletal system must limit motor mode of the patient, the use of special orthopedic equipment to reduce load on the skeleton.

In the experiment on rats after shock wave stimulation of the note expressed the plethora of vessels of the terminal vascular bed in the impact zone due to the expansion of capillaries and arterioles. However, what is needed is the growth of new vessels of the microvasculature (neoangiogenesis), this effect known method can not provide.

The disadvantage is the high cost of equipment for stimulation and treatment procedures.

The main m the SSA created and used vehicles for exposure to magnetic fields (MF) is focused on the range of induction MP from fractions to 50-60 MT. In recent years, experts have begun to intensively study the impact of strong MP, up to units of T, tissues and organs. While often focus on short-time pulse MP, usually lasting no more than a few milliseconds (usually shares). The main principles of the known devices is the accumulation of electrical energy in the capacitor and subsequent intermittent discharge the inductor, creating near a pulsed magnetic field (UTI). This is a sharp increase in current in the inductor and exponential declines. The character of the PULSE is not the best way affects the tissues of the body, because it is more preferred that structure fields (corresponding shape of the current pulse), which provides a more physiological effect on the body. You can count on more tangible effect of MP.

A device for pulsed magnetic field that contains a master oscillator connected to the timer, the shaper and amplifier generated pulses is connected to the power supply to the amplifier output power of the generated pulses with the cable connected to the magnetic inductor (see www.neurosoft.ru/rus/product/neuro-ms//). In the case of devices placed power part and precision electronics control unit. WPI is the local device is a system of capacitors high voltage (from 400 up to 3.5 kV) and high current (from 4 kA to 20 kA and more) which are discharged through the stimulation coil of copper wire (the so-called "inductor"). The inductor varies in shape and size. So, in the circular inductors the area of maximum magnetic induction is at the inner edge (edge of the inner circle), planar inductors in the center of the working surface. The inductors in the form of "eight" (or butterfly) maximum field falls on the center of the inductor (the area where contact both "wing"), which allows to obtain a well-focused, but relatively weak PULSE. Small inductors create a high tension MT at the surface of the skin and therefore, as the inductors butterflies, good for impact on surface structure, large round coil creates a strong, deep penetrating fields, but their impact is poorly focused. The keyboard allows you to manage all aspects of magnetic stimulation, when the unit is offline. Digital indicators display all parameters of the pacemaker, the indicator - temperature inductor, it is possible to supply the stimulus by pressing one button on the inductor.

General parameters and characteristics of the Neuro-MS:

- power supply - 220-230V (50 Hz);

- power consumption - no more than 1,000 watts;

- number of channels - 8.

Options magnetic stimulation:

- adjustment range: amplitude m is Gnanou induction - 0.3 to 2.2 T;

the pulse duration of 200 µs;

the pulse frequency at the maximum magnetic induction - 5 Hz;

- the range of tasks: the pulse repetition rate of 0.1 - 30 Hz;

- the range of tasks time: in the regime of periodic stimulation of 0.5-10 min;

the amplitude of the magnetic induction pulse stimulation (depends on the type of inducer);

the shape of the pulse is biphasic;

- the duration of a pause between the next series of incentives - 0-300;

- the duration of the stimulation session - 0.5 to 30 minutes

In the known device (Neuro-MS) using a short magnetic pulses. Emerging electromagnetic field of high intensity can freely penetrate through clothing, skull bones and soft tissue and affects the deeper nerves, peripheral nerves, brain and spinal cord, is not available for other kinds of stimulation.

The known device is intended for neurology treatment of lesions of the Central and peripheral nervous system, vascular and vertebrogenic diseases, neuropathies, trauma - treatment of traumatic injuries to the bones, spine, muscles, joints, spinal osteochondrosis and its complications in ophthalmology - the use of partial atrophy of the optic nerve due to any etiology. "Neuro-MS used in psychiatry, epilepsy, pediat the AI and child neurology, pulmonology.

However, with all the efficiency of the known device has several disadvantages:

- the impossibility of penetration of pulsing MP in the deep structure of the body covering the desired volume of irradiated tissue;

the inability of the effective use of the known device for stimulation of metabolic processes in biological tissues due to a lack of physiologic forms of pulse MP, but is suitable and effective for use in neurology.

The objective of the proposed group of inventions is to improve the efficiency of stimulation of the vital processes in organs and tissues, not only in the area most pronounced trophic signs, but in the intact areas (skin, muscle) repair of vessels of the microvasculature and the creation of devices that can improve the efficiency of these processes through the magnetised necessary plots powerful pulsed magnetic field.

The problem is solved in that in the known method of stimulation of metabolic processes in biological tissues, including the effects of pulsed magnetic field through the surface of the biological object to the area of stimulation, the effects of exercise pulsed magnetic field by imposing inductor North pole on the areas of most pronounced trophic characteristics, is roccio distal aorta, iliac and femoral arteries (three courses for 7 days daily at intervals of 5 days, and during the first 2 days of the first year impact carried out for 7 minutes with a frequency of incentives 5 Hz, with an amplitude value supplied to the inductor voltage 600 V and within 3 minutes with a frequency of stimuli of 1 Hz, with a voltage of 600 V, the final two days of the voltage increase up to 650 V and the exposure time to 1 minute as the frequency of the stimulus 5 Hz and 1 Hz, during the following three days the first cycle, the voltage is increased to 700 and In 1 minute exposure time at both frequencies, during the second year of exposure sessions performed with a voltage of 700 V, the exposure time increases when exposed to both frequencies on the third, fifth and seventh sessions, the third course spend every day with a voltage of 700 V and a maximum exposure time of 10 minutes with a frequency of incentives 5 Hz and 6 minutes with a frequency of stimuli of 1 Hz.

The problem is solved in that in the known device for the pulsed magnetic field that contains a master oscillator connected to the timer, the shaper and amplifier generated pulses is connected to the power supply to the amplifier output power of the generated pulses with the cable connected to the magnetic coil, the coil is a flat coil with rasmusen the m in the same plane coaxially closed loop.

The proposed device is illustrated graphic material.

In Fig. 1 depicts a block diagram of the proposed device.

The device comprises a master oscillator 1 low-frequency pulse switchable asked frequencies of 1 Hz, 5 Hz or 10 Hz, coupled with programmable timer 2, which sets the exposure time of low-frequency pulsed electromagnetic field from 1 to 10 minutes. The timer is attached shaper and amplifier 3 power generated pulses to control the voltage amplitude of the amplified pulses in the range from 300 to 800 V, the output of which cable is connected to the magnetic coil 4 made in the form of a flat coil placed coaxially in the same plane with her closed loop 5. The device contains the necessary unit 6 AC power. On the front panel of the device there are elements of the job required frequency, exposure time, voltage inductor and display the values of selected parameters.

In mode 5 Hz the capacitor discharge is incomplete, that with some decrease in the amplitude of the pulse creates some constant component of the current and magnetic field, respectively. The inductor is connected to the working side of the inductor was converted to the hot zone of the Northern floor of the som. And he, as you know, helps reduce blood viscosity. When this stimulation processes are active. At a frequency of 1 Hz pulse and its energy is larger, which enhances subsequent exposure IMP. The maximum induction of the field on the working surface of the inductor (coil is made flat) takes place near the center of the inductor, at the edge of the center hole of the coil. Flat coil provides the penetration of the magnetic field at a greater distance along its axis, which allows to reach the treated area of the biological tissue at a greater depth with the required level of induction MP.

Technical characteristics of the proposed device:

- power supply 220-230 V;

- power consumption : 1000 W;

- number of channels - 1;

the amplitude of the magnetic induction from 1 to 2 T;

- pulse duration - up to 1 MS;

- working frequency - 1, 5, 10 Hz;

- set the exposure time from 1 to 10 minutes.

the amplitude of the voltage pulse to the inductor from 100 to 800 C.

The proposed group of inventions meet the criteria of "novelty" and "inventive step", as held patent information research did not reveal sources of scientific, technical and patent information prejudicial to the novelty of the proposed method and device, as well as technical solutions with significant signs of p is izlagaemykh technical solutions.

Frequency selection incentives 5 Hz due to the fact that, according to the literature, this frequency is consistent with internalnotify of the cardiovascular system (CVS) and the peripheral nervous system (NS), providing activate the circulatory and lymphatic vessels, peripheral NS, skeletal muscles and, accordingly, the normalization of tissue metabolism. To provide the power and energy impact on the area of interest (diseased tissue), exposure was performed with pulses with a frequency stimuli of 1 Hz. This internalnet - 3 course daily exposure for 7 days caused, on the one hand, the need for multiple repetitions of exposure for the emergence of sustainable signs of activation of blood flow and changes in the macroscopic characteristics of the nidus, and on the other hand, the need to mitigate the effect of habituation. The interval of 5 days between the rates of exposure PULSES is chosen so that the body does not have time to adapt to the applied electromagnetic effects. The impact frequency of the stimulus 5 Hz for a minimum of 7 minutes and a maximum of 10 minutes is required to achieve maximum stimulating effect in biological tissues. Impact frequency stimuli (1 Hz) for at least 3 minutes and a maximum of 6 minutes provides more powerful the energy impact due to more complete discharge of the storage capacitor to the inductor.

The proposed group of inventions provides the following technical effects:

- to increase the effectiveness of the stimulation of the vital processes in organs and tissues, not only in the area most pronounced trophic signs, but in the intact areas (skin, muscle) repair of vessels of microcirculation by increasing the efficiency of the process of magnetization that biological tissues.

The execution of the inductor in the form of a flat coil placed on the same plane coaxially closed loop allows you to create more physiological impulse affecting the magnetic field and provides deep penetration of the magnetic field in the receptors. The device creates in the working zone of the induction magnetic field up to 2 T pulse.

Given the unipolar nature of the supply, the inductor voltage is magnetic polarization. Connecting the inductor to the output of the amplifier pulse generator is such that on the side of the working surface of the inductor will always have a place in the North pole. This is necessary because it is known that the North pole of the magnetic field thins the blood, and the southern thicken. This effect is important in the implementation of tasks.

To assess the effectiveness of the proposed device and check the efficiency of the method were Prov is found experimental research.

During the experiment were grown in 6 rabbits with a particular mode of feeding, including cholesterol, to obtain an animal model of atherosclerotic vascular lesions. All rabbits in the process of feeding was formed trophic ulcers on the extremities (mainly the back), was clearly observed symptoms of hair loss (on the ears, neck and limbs) and obesity (picture). All animals was performed angiography of the aorta, was diagnosed with diffuse atherosclerotic lesion of the vessel. During the second phase of the experiment were formed 2 groups of 3 animals: the main and control. In the control group animal no impacts was not performed. All animals were irradiated by a pulsed alternating electromagnetic field is maximally expressed trophic changes that are localized to the plantar surface of the feet of the hind limbs, and also worked in the projection of the distal aorta, iliac vessels and femoral.

Was performed 3 series of exposures with an interval of 5 days. The study was carried out monitoring of animals, which was evaluated by visual characteristics:

- the condition of a scalp and the growth of claws;

- the presence, depth and size of the trophic ulcers;

- the existence and nature of granulations and Capitalizati trophic I the century

After 4 months from start of feeding, inducing atherosclerosis, animals removed from the experiment. Visual examination it was shown that in contrast to the progressive balding areas of the skin in animals of the control group is restoring hair growth in animals exposed to the PULSE of the proposed method. In comparison with the control group in irradiated animals normalization of growth of the claws of the hind limbs. Also revealed an increase in the diameter of blood vessels, compared with those before irradiation in animals exposed to PULSE. In the control group of animals was no visible expansion of the subcutaneous vessels. In the control group was a progressive increase in the total area of trophic ulcers during the whole experiment. In irradiated animals in response to the impact of the total area of trophic ulcers decreased, decreased depth of the ulcer is due to the emergence of active granulation bright pink color over the entire surface of the ulcer. Starting from the third level of the selected contact bleeding from granulating areas, which indicates the improvement of blood circulation. By the end of the course of irradiation on the perimeter of trophic defects were marginal epithelization. Performed histological examination of tissues from the zone of the maximum is s trophic disorders (color Hematoxylin-eosin) using a light microscope LeicaDMD 108 (Germany) with a resolution of 1280x960 (for panning). Histological examination showed increase in the number of active macrophages in the tissues, partial involution of atherosclerotic plaques, neoangiogenesis (new vessels), the normalization of the structure of the tissue (picture). Pathomorphological picture of the ear: in the area of the Central artery of the inner surface of the ear of the rabbit there is moderate swelling of the dermis. The wall of the Central artery in heightened tone with irregular folding of the surface. There are lots intima with potentielleme vacuoles. In the lumen of the artery contains erythrocytes. Thus, the proposed group of inventions can significantly offset the local manifestations of atherosclerotic lesions until complete healing of venous ulcers.

Healing simulated trophic ulcers in the impact zone:

- growth of coarse-fibered connective tissue in the dermis and subcutaneous fat layer.

ULCER

- reducing the total area of trophic ulcers;

- contact bleeding from advanced multiple blood vessels of the terminal vascular beds;

- reducing the depth of the ulcer.

MUSCLE, LEATHER THIGH

- normalization and trophic structure of the epidermis, the dermis;

- the epidermis is correctly formed with the usual location of the cell is Loew;

- increase the number of active macrophages in the tissues;

- pronounced elastic frame;

the abundance of blood vessels terminal vascular beds;

- growth of skin appendages: hair and claws;

- the emergence of new vessels of the microvasculature.

1. Neoangiogenesis in the affected area (skin, subcutaneous fat layer, muscle):

partial involution of atherosclerotic plaques;

- the vessel wall in the impact zone in a state of heightened tone with uneven folding surface and intimal areas with potentielleme vacuoles;

in the lumen of the vessels containing erythrocytes.

2. The appearance of granulation tissue in the impact zone:

active granulation bright pink color over the entire surface of the ulcer.

The proposed method using the proposed device is as follows.

Exposure is carried out by applying pulse inductor's magnetic field North pole on top of the areas of most pronounced trophic traits to the projection of the distal aorta and iliac femoral arteries.

1 day first perform a pulsed magnetic field to the area of the ulcer with the frequency of the stimulus 5 Hz for 7 minutes, with an amplitude value supplied to the inductor voltage 600 V. For the eat IMP act on the same zone with the frequency of the stimuli of 1 Hz for 3 minutes with an amplitude value supplied to the inductor voltage 600 C.

day 2 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 7 minutes, voltage 600 V, then the influence of PULSE frequency stimuli of 1 Hz for 3 minutes and a voltage of 600 C.

day 3 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 8 minutes and a voltage of 650 V, then the influence of PULSE frequency stimuli of 1 Hz for 4 minutes and a voltage of 650 C.

day 4 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 8 minutes and a voltage of 650 V, then the influence of PULSE frequency stimuli of 1 Hz for 4 minutes and a voltage of 650 C.

day 5 - influence of PULSE frequency stimuli 5 Hz for 9 minutes and the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 5 minutes at a voltage of 700 C.

day 6 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 9 minutes and a voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 5 minutes at a voltage of 700 C.

day 7 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 10 minutes at a voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 6 minutes at a voltage of 700 C.

BREAK FOR 5 DAYS

The second course implementing impact PULSE within 7 days with an amplitude value supplied to the inductor voltage 700 C.

day 1 - influence of PULSE frequency stimuli 5 Hz for 7 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz in accordance with the s 3 minutes voltage 700 C.

day 2 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 7 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 3 minutes, the voltage of 700 C.

day 3 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 8 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 4 minutes, the voltage of 700 C.

day 4 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 8 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 4 minutes, the voltage of 700 C.

day 5 - influence of PULSE frequency stimuli 5 Hz for 9 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz within 5 minutes, the voltage of 700 C.

day 6 - the impact of the PULSE with the frequency of the stimulus 5 Hz for 9 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz within 5 minutes, the voltage of 700 C.

day 7 - the impact of the PULSE with the frequency of the stimulus 5 Hz within 10 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 6 minutes, the voltage of 700 C.

BREAK FOR 5 DAYS

Third course - the impact of perform within 7 days with an amplitude value supplied to the inductor pulse voltage of 700 V and a maximum time of exposure.

day 1 - influence of PULSE frequency stimuli 5 Hz within 10 minutes, the voltage of 700 V, then the impact of the IMP with h which depends stimuli of 1 Hz for 6 minutes, voltage 700 C.

day 2 - the impact of the PULSE with the frequency of the stimulus 5 Hz within 10 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 6 minutes, the voltage of 700 C.

day 3 - the impact of the PULSE with the frequency of the stimulus 5 Hz within 10 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 6 minutes, the voltage of 700 C.

day 4 - the impact of the PULSE with the frequency of the stimulus 5 Hz within 10 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 6 minutes, the voltage of 700 C.

day 5 - the influence of pulsed magnetic field with the frequency of the stimulus 5 Hz within 10 minutes, the voltage of 700 V, then the influence of pulsed magnetic field with a frequency stimuli of 1 Hz for 6 minutes, the voltage of 700 C.

day 6 - the impact of the PULSE with the frequency of the stimulus 5 Hz within 10 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 6 minutes, the voltage of 700 C.

day 7 - the impact of the PULSE with the frequency of the stimulus 5 Hz within 10 minutes, the voltage of 700 V, then the influence of PULSE frequency stimuli of 1 Hz for 6 minutes, the voltage of 700 C.

A specific example of implementation is given in the form of the test.

Protocol 1.

Six rabbits "chinchilla" weight of from 2500 to 3400 g was subjected to a special mode of feeding (holestirinovye diet), including cholesterin, with the aim of obtaining animal models and erosclerosis lesions of blood vessels. After 3 months of feeding all animals were formed trophic ulcers on the extremities (mainly the back), was clearly observed symptoms of hair loss (on the ears, neck and limbs) and obesity. After the angiography of the aorta diagnosed with diffuse atherosclerotic vascular lesions.

All animals were irradiated PULSE using the proposed device. The exposure was carried out by overlaying the inductor of the device is the North pole on the areas of most pronounced trophic changes that are localized to the plantar surface of the feet of the hind limbs, and also affect the projection of the distal aorta, iliac and femoral vessels. Impact conducted three courses for 7 days daily intervals between courses of 5 days. First course: the first 2 days exposure was carried out for 7 minutes with a frequency of incentives 5 Hz, with an amplitude value supplied to the inductor voltage 600 V and within 3 minutes with a frequency of stimuli of 1 Hz, a voltage of 600. In the next two days, the voltage was increased to 650 V and the exposure time to 1 minute as the frequency of the stimulus 5 Hz, and the frequency of the stimuli of 1 Hz. During the following three days the first cycle, the voltage was increased to 700 V and the exposure time at both frequencies increased to 1 minute. Then follow the al a break for 5 days. During the second year of exposure sessions were conducted with a voltage of 700 V, the exposure time is increased to 1 minute when exposed to both frequencies on the third, fifth and seventh sessions. The third course was carried out daily with a voltage of 700 V and a maximum exposure time of 10 minutes with a frequency of incentives 6 Hz and 6 minutes with a frequency of stimuli of 1 Hz.

After 4 months from start of feeding, inducing atherosclerosis, the animals were taken out of the experiment.

Visual examination it was shown that animals exposed to radiation, it was the restoration of hair growth, showed normalization of growth of the claws of the hind limbs, revealed an increase in the diameter of blood vessels by 20-40% relative to the source, the total area of venous ulcers was reduced by 70%-80%, with reduced depth ulcers (80%) due to the emergence of active granulation bright pink color over the entire surface of the ulcer.

Starting from the third year of exposure to the selected contact bleeding from granulating areas that showed improvement of blood supply of tissues.

By the end of the course of irradiation on the perimeter of trophic defects were marginal epithelization.

Performed histological examination of tissues from the zone of maximum trophic disorders (color Hematoxylin-Eosin) using light is microscope Leica DMD108 (Germany).

Histological examination showed increase in the number of active macrophages in the tissues, partial involution of atherosclerotic plaques, neoangiogenesis (new vessels), the normalization of the fabric structure.

Held OCT study confirmed according to histological study in the area of trophic ulcers, as well as the widening of the blood vessels of the microvasculature in the surrounding intact sites (skin, muscle), referativnyi processes surrounding the ulcer tissue.

Example 2.

Auricle two intact rabbits "chinchilla" weight of from 2500 to 3400 g was subjected to irradiation by a pulsed alternating magnetic field on the proposed method using the proposed device.

After the 3 courses impact the animals were taken out of the experiment.

Pathomorphological picture of the auricle was as follows: expressed neoangiogenesis in the center and on the periphery of the ear, in the area of the Central artery of the inner surface of the ear of the rabbit there is moderate swelling of the dermis. The wall of the Central artery in heightened tone with irregular folding of the surface. There are lots intima with potentielleme vacuoles. In the lumen of the artery contains erythrocytes.

The method of stimulation of metabolic processes in biological tissues in e is speriment, including the effects of pulsed magnetic field, characterized in that the exposure is carried out by blending the inductor North pole on the areas of most pronounced trophic characteristics, the projection of the distal aorta, iliac and femoral arteries (three courses for 7 days daily at intervals of 5 days, the first 2 days of the first year impact carried out for 7 minutes with a frequency of incentives 5 Hz, with an amplitude value supplied to the inductor voltage 600 V and within 3 minutes with a frequency of stimuli of 1 Hz, with a voltage of 600 V, the next two days, the voltage increase up to 650 V and the exposure time 1 minute on the frequency of the stimulus 5 Hz and 1 Hz, the next three days the first cycle, the voltage is increased to 700 and 1 minute exposure time at both frequencies, during the second year of exposure sessions performed with a voltage of 700 V, the exposure time increases when exposed to both frequencies on the third, fifth and seventh sessions, the third course spend every day with a voltage of 700 V and a maximum exposure time of 10 minutes with a frequency of incentives 5 Hz and 6 minutes with a frequency of stimuli of 1 Hz.