Method for producing diagnostic allergen |
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IPC classes for russian patent Method for producing diagnostic allergen (RU 2428197):
Early diagnostic technique for clinical course and outcome of neurotic, stress-related connected with stress, somatoform and neurosis-like disorders / 2427841
Ratios of immunological and biochemical homeostasis values are calculated. If observing an immunoregulatory index less than 1.5 units, a mature form density coefficient in T-lymphocyte population less than 0.8 units, an antibody production intensity coefficient of B-cells less than 2.5 units, an antioxidant protection coefficient less than 3.25 units If observing the absence or poor acute phase protein reaction with underlying Garkavi adverse adaptive reactions of acute or chronic stress, unfavourable course of the mental disorder is diagnosed.
Method for prediction of developing complications in women suffering climacteric syndrome and mastopathy and employing hormon replacement / 2426994
Peripheral blood of postmenopausal women suffering climacteric syndrome is examined for a pre-treatment fraction of interleukin-6 positive lymphocytes with underlying hormone replacement. If observing the concentration of interleukin-6 positive lymphocytes being equal to or more than 6.9 %, mastodynia and/or mastalgia is predicted in women suffering chronic cystic mastitis with underlying Femoston 1/5 or Climodiene treatment.
 Method of structural description of recombinant polyclonal protein or polyclonal cell line / 2426795
Method includes analysing aliquots of said sample by one or more methods of protein description specified in the chromatography. The method is based on genetic analysis techniques specified in RFLP and T-RFLP. These methods can be applied both separately, and in a combination. The offered methods allow obtaining the information on the presence and fractions of various individual proteins or coding sequences. The obtained information can be used for evaluating stability of a polyclonal cell line in process, and also estimating a structure of various parties of end polyclonal products.
 Method of determining iga-proteinase activity / 2426126
Immunoglobulin-A is sorbed in microplate wells, then after incubation and treatment, a solution containing a proteolytic enzyme is introduced in the microtablet wells. It is followed with incubation; the contents of the wells are poured out, and then an enzyme conjugate, e.g. peroxidase, with immunoglobulin-A Fc-fragment antibodies and a substratum of this enzyme are introduced. After incubation of a reaction mixture, reaction results are accounted by a spectrophotometre. IgA-protease activity is calculated by the amount of hydrolysed substratum of the enzymatic reaction.
Method for prediction of developing pathological cicatrisation following cheilo- and cheilorhinoplasty in children / 2426125
What is offered is a method for prediction of developing pathological cicatrisation shown by preoperative venous blood examination. An electrophoresis method is used to analyse blood serum proteins and to record Igl fraction concentration of alpha-2 globulins. Also, the diastasis value L of the maxillary dental arch fragments is measured. A score of the derived values is used to predict three possible outcomes: favourable course of postoperative cicatrisation, a moderate risk of developing pathological cicatrisation, a high risk of developing pathological cicatrisation.
 Method of prediction of prevalence of stenotic transplant coronary bed lesion in recipients following cardiac transplantation / 2426124
Blood plasma is analysed for a cardiolipin antibody level. If at least two studies has shown an upper limit of the reference blood plasma cardiolipin antibody limits exceeded by 10-20%, the one-vascular stenotic lesion is predicted. The multivascular stenotic bed lesion is proven by at least two studies showing an upper limit of the reference blood plasma cardiolipin antibody limits exceeded by more than 21%.
 Method and kit for immune-enzyme functional activity assay on c1r2s2 subcomponents of first component of human complement / 2425383
Invention represents a method of evaluating functional activity of Clr2s2 subcomponents of the first component of a human complement involving sorption in microplate wells of a classical complement cascade, introduction of a solution containing porpoise serum and sodium ethylene diamine tetraacetate; then incubation and drying of the plate is followed by introducing the analysed sample containing the Clr2s2 complex of the human complement of unknown activity, and a pseudoglobulin fraction of human blood serum prepared by distilled water dialysis (R1), and also a buffer solution containing calcium and magnesium ions, incubation, and after washing and drying of the plate, introduction of a enzyme conjugate with human C3 component and substratum antibodies into the wells, calculation of Clr2s2 subcomponent activity by an amount of the produced enzyme reaction product, differing by the fact that the classical complement cascade is derinate, and also a kit for functional activity assay on Clr2s2 subcomponents of the first component of the human complement.
 Method of blood plasma proximate analysis for cardiomyoglobin by means of electrochemical immunosensor / 2425382
Method of blood plasma proximate analysis for cardiomyoglobin by means of an electrochemical immunosensor consists in the fact that a blood plasma sample is applied on a surface of a main electrode pre-modified by colloidal gold and monoclonal cardiomyoglobin antibodies; the prepared sample immunosensor is kept, preincubated in a buffer solution; cardiomyoglobin is electrochemically recorded in the sample by the prepared calibration diagram under certain conditions.
 Method of evaluating immunosuppressive response rate in children with chronic glomerulonephritis / 2425381
Invention describes a method of evaluating an immunosuppressive response rate in children with chronic glomerulonephritis characterised by determining the peripheral blood cell kinetics by flow DNA-cytometry; evaluating a cell fraction in a proliferation phases not less than 0.74% enables to consider the immunosuppressive therapy to be prescribed, and observing a decreased cell level in proliferation phases in 2-3 weeks after the beginning of administration at least in 2 times, the therapy is considered to be effective; the absence of positive dynamics requires dosage change or preparation replacement.
Method of placental homogenate analysis for heparin concentration in puerperants underwent acute herpes viral infection / 2425372
Invention describes a method of evaluating the heparin concentration in puerperants underwent acute herpes viral infection in the third trimester, characterised by the fact that a piece of placenta is sampled from the puerperants, homogenate is prepared and processed for glycosaminoglycans recovery. Thereafter, the prepared glycosaminoglycans extracts are separated by gel electrophoresis in a polyacrylamide gel, and a heparin percentage is calculated by optical density sensitometry; a herpes viral infective episode is stated by spectrophotometry and shown by increasing titre of herpes virus antibodies.
Method of obtaining preparation for carrying out application skin test / 2423144
Invention relates to field of biotechnology, and describes method of obtaining preparation for carrying out application skin test, which includes mixing tested substance with carrier and solvent, as carrier used is gar-agar, as solvent - borate buffer pH 6.8-7.2, agar-agar in borate buffer is heated with mixing to complete dissolution of agar-agar, mixture is cooled to 40-45°C and tested substance in amount 10-50% of total volume of ready preparation is introduced, as tested substances used are their water solutions with concentrations, capable of inducing contact reactions of hypersensitivity.
 Cat allergen fused protein and application thereof / 2414239
Invention refers to medicine and concerns cat allergen fused proteins and application thereof. Substance of the invention involves a compositions containing a virus-like particle (VLP) or a viral particle and at least, one antigen, first of all at least, one cat antigen, and more specifically at least one cat antigen which is human allergen. In specific versions of the invention, said antigen represents cat antigen Fel d1 or its fragment covalently bound with the VLP.
Complex allergen for differentiating dried purified tuberculine allergic reactions for mammals / 2409387
Invention concerns veterinary medicine. Currently, for differentiating nonspecific tuberculin reactions in cattle, dried purified tuberculin is used for mammals and "КАМ" with considering the sensitisation pattern by the reaction intensity. A common complex allergen is produced by protein settling from M scrofulaceum No. 12-C and M intracellulare No. 13-H strain cultures with added allergen produced from Corynebacterium xerosis N1911, in amount 1350 units of activity. The presence of coryneformic bacteria allergen in the "КАМ" composition improves the efficacy of a simultaneous dried purified tuberculin test for mammals in differentiating the nonspecific coryneformic bacteria reactions.
Method of treating allergic rhinitis of domestic etiology / 2394595
Invention relates to medicine, namely to allergology, and can be applied for treatment of allergic rhinitis of domestic etiology. For this purpose in treatment included is allergen-specific immunotherapy with sublingual introduction of cause-significant allergen. Simultaneously hirudotherapy with application of medical leech on skin in region of oral cavity bottom projection is carried out. Procedures are carried out 2 times per week, starting from 0.1 ml 10-6 degree of dissolving of cause-significant allergen and bringing up to 0.5 ml of undissolved allergen, which after that is introduced 1 time per 2 weeks during 3 years.
 Allergen-containing dosage form / 2372074
Present invention concerns medical products, particularly a pharmaceutical product for introduction of allergen that contains high-soluble solid dosage form introduced through tunica mucosa of mouth and containing matrix made by freeze drying from the solution containing 3-6.5 wt % pre-gelled starch and 3-5.5 wt % mannitol, or from the solution containing 2-10 wt % fish gelatine and 1-10 wt % mannitol; and allergen. Besides, invention concerns the method for making the specified product, a multidosage container, a set for allergy treatment, to the method of treating and applying the specified product.
Method of producing house dust mite (dermatophagoides) allergen / 2331437
Method comprise salt-water extraction from raw material using Evans-Cook extraction liquid, mites cultivation during from 3 to 4 months, at temperature 25±2°C and relative air humidity 73±3%, on house dust and bristle. As initial basis, the mix of house dust mites Dermatophagoides farina or Dermatophagoides pternissinus culture and culture medium, including excrements, cast off skins, and food debris, is used. Salt-water extraction from raw material is performed during three days, after mixing it and homogenization with glass powder; the maintained incubation temperature is 2° to 10°C; the extraction material being shaken for 30 minutes three times a day with 1.5 hour intervals. Extraction completed, the supernatant liquor is decanted and centrifuged at 5000 to 6000 rpm for 30-40 minutes, then it is filtered through paper filter; in consequence sterilization through filtering, 3 to 4 months mother solution stabilisation, and bottling are carried out; the finished preparation is produced.
 Microparticles containing carbohydrate spheres covalently bonded with allergen / 2329830
Present invention refers to medicine and concerns microparticles containing sphere mainly consisting of cross-linked agarose carbohydrate and allergen covalently bonded with sphere, applied for immune system disturbance treatment. Used allergen is produced of plant pollen, specifically of timothy grass pollen. Application of specified microparticles provides effective treatment for patients suffering from allergy, as well as reduces by-effects of parenteral introduction.
 Conjugates of hydroxyalkyl starch and allergen / 2325925
Invention refers to medicine, specifically to conjugates of hydroxyalkyl starch and allergen, in which at least one hydroxyalkyl starch is covalent bonded with allergen for hyposensitisation. Conjugates HAS-allergen provide epitope profile comparable to natural, that enables to increase immunotherapy efficiency.
Method for predicting tuberculosis in cattle / 2314826
The innovation deals with intracutaneous injection of tuberculin at the volume of 0.2 ml followed by analyzing the reaction 72 h later. Animals at increased skin fold by 3 mm and more should be considered to be sick ones. Moreover, tuberculin should be injected at the dosage ranged 0.6-0.8 thousand IU. The innovation excludes the impact upon final result in predicting the factors of tuberculosis-free etiology and provides the liquidation of groundless slaughtering in cattle.
Method for production of diagnostic allergen / 2311925
Claimed method includes alkali extraction of raw materials followed by centrifugation, filtering, and sterilizing filtering of target product, wherein as raw material mixture of frozen cockroaches is used. Said mixture is crushed, then ether is added, mixture is shacked, filtered, dried to full ether evaporation, grated to produce powder. In obtained powder ether is added again, powder is dried to full ether evaporation, and alkali extraction is carried out. Extracting material is agitated and for 3 days is shacked three times daily for 30 min with 1.5 h interval and stored for these intervals at 2-10°C. After extraction supernatant is discharged, centrifuged, and after sterilizing filtering sterile allergen solution is held for 1-3 months at 2-10°C.
 Chinese therapeutic composition, therapeutic insect extracts contained in containing and methods of preparing composition / 2427381
Invention refers to pharmaceutical industry, particularly to an agent for treating cardiovascular and cerebrovascular diseases. A method of preparing a therapeutic composition for treating cardiovascular and cerebrovascular diseases that involves selection of scorpion, centipede, eupolyphaga or steleophaga, leech, cicadas, ginseng root, prepared incense, peony root, sweet and white sandal wood, boras camphor and Ziziphi spinosae seeds according to the preset amounts, preparation of crude therapeutic extracts of said initial materials, agitation of the prepared extracts. A product of the therapeutic composition for treating cardiovascular and cerebrovascular diseases. A method of preparing the therapeutic insect extracts for treating cardiovascular and cerebrovascular diseases. The therapeutic insect extracts for treating cardiovascular and cerebrovascular diseases.
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FIELD: medicine, pharmaceutics. SUBSTANCE: invention refers to pharmacology and medicine, namely to a method for producing diagnostic allergens of a number of synanthropic insects, such as: Blattella germanica, Blatta orientalis, Pertplaneta americana cockroach Neuphoeta cinerea, Musca domestica, Tinea pelionella, Cimex lectularis, Monomorium pharaonis, Tegenaria derhami, Culex pipiens, Attagenus Smirnovi Zhan. For the method for producing the diagnostic allergen, initial raw materials are frozen synanthropic insects. The initial raw materials are grinded to particle size 1-3 mm, degreased by addition of ether in the ratio 1:3-4 to the raw materials; the raw materials are kept in ether during 15-20 min that is followed by removal of ether by mixture filtering; the raw materials are degreased again by ether, dried to complete ether removal; the completely fat-free raw materials are powdered. The prepared powder is extracted in two stages; the first stage involves pouring the powder with an extractant liquid 1:1 and mixing to a homogeneous suspension; and the second stage includes mixing the prepared suspension with the extractant liquid in the ratio 2:1 and agitating by shaking the suspension three times for 30 minutes every 1.5 hours at temperature 2-10°C for 3 days with daily correction of the pH value of the allergenic extract to 7.0-7.2. EFFECT: invention provides extended range of the diagnostic allergens and creation of a highly effective preparation for the diagnostic diseases caused by sensitisation to synanthropic insects. 2 cl, 1 ex, 1 tbl
The invention relates to pharmacology and medicine, particularly to a method of obtaining diagnostic allergens from synanthropic insects, such as red cockroach (Blattella germanica), black cockroach (Blatta orientalis), the American cockroach (Periplaneta americana), cockroach ash (marble) (Neuphoeta cinerea), the fly room (Musca domestica), mol a (Tinea pelionella), the bed bug (Cimex lectularis), red ant house (Monomorium pharaonis), the house spider (Tegenaria derhami), the ordinary mosquito (Culex pipiens), Majeed (Attagenus Smirnovi Zhan). A method of obtaining tick allergen for setting diagnostic tests and treatment, including water-salt extraction of raw materials and the processing of formaldehyde, as a source of raw materials used culture house dust mites Dermatophagoides Farina or Dermatophagoides pteronyssinus, extraction with a solution of ammonium hydrogen carbonate, centrifugation for 45 min, filtering the extract, dialysis for 52 hours at a temperature of 6±2°C, twice for 24 h against a 0.002 M solution of ammonium hydrogen carbonate and once within 4 hours against distilled water, centrifuging cialisovernight extract for 1 h, filter it through paper the filter holding freeze drying to a residual moisture content of not more than 4%, dissolving the dry residue in sterile conditions in 0.1 M phosphate buffer with pH 7.5, add the giving of 1%aqueous solution of formaldehyde, holding sterilizing filtration, keeping formalisations of allergoids for 32 days at a temperature of 32°C, followed by dialysis at a temperature of 6±2°C against 0.1 M phosphate buffer solution with pH 7.5 to obtain the final form of the allergen to diagnose (EN 2265450, A61K 39/00, 2005). The disadvantage of this method is the limited action of the allergen. The main cause of year-round respiratory allergies (asthma, allergic bronchitis, allergic conjunctivitis and other) are the allergens of man's dwelling. In the main part of them is house dust is composed of waste products of various organisms, satellites biocenosis person: mites, insects, Pets, etc. the Last decade, researchers have drawn attention as a cause of allergies, synanthropic insects, which are found in man in all regions of the world. It is shown that 60-80% of patients with bronchial asthma sensitized to the cockroaches, in 10-29% of cases are positive skin tests to extract flies. Allergic reactions to the bites of blood-sucking insects have 17-20% violence. Maximum figures are for the United States and Japan, is somewhat lower for countries in the Middle East, Israel. In the CIS such studies were not conducted, and euda only indirect information about the high prevalence of IgE antibodies to cockroach in children however, they are obtained with the help of foreign test systems. The main reason is the lack of domestic preparations of allergens. On the other hand drugs abroad are mostly ad hoc and need more detailed physico-chemical characterization and standardization. This work is being actively carried out abroad. For the diagnosis of hypersensitivity to synanthropic insects necessary preparations of allergens, which can be used for the production of skin samples and for use in the reactions in vitro. Such preparations allergens in domestic allergological practice no. Expanding the range of manufactured drugs for the diagnosis of allergic conditions is an important task of practical health care. For drugs that are needed for solving this problem include allergens from synanthropic insects developed in SU NIELS them. Mechnikov RAMS. The technical result of the invention is to expand the range of diagnostic allergens and the development of highly effective drugs for the diagnosis of diseases caused by sensitization to synanthropic insects such as the red cockroach (Blattella germanica), black cockroach (Blatta orientalis), the American cockroach (Periplaneta americana), cockroach ash is hydrated (marble) (Neuphoeta cinerea), the fly room (Musca domestica), mol a (Tinea pelionella), the bed bug (Cimex lectularis), red ant house (Monomorium pharaonis), the house spider (Tegenaria derhami), the ordinary mosquito (Culex pipiens), Majeed (Attagenus Smirnovi Zhan). To achieve the technical result in the method of obtaining diagnostic allergen, comprising the alkaline extraction of raw materials, centrifuging, filtering and sterilizing filtration to obtain the final form of diagnostic allergen, according to the invention as a source of raw materials used frozen synanthropic insects, crushed raw materials on the particle size of 1-3 mm, degreased crushed raw material by adding in raw materials ethyl ester in a ratio of 1:3-4, sustain raw material in ethyl ether for 15-20 min with subsequent removal of the ether by filtration of the mixture, again degrease raw materials ethyl ether, dried to remove the ether, crushed completely skimmed raw materials to powder and the extraction of the obtained powder is carried out in two stages, the first of which poured the powder extracting liquid 1:1 and stirred until a homogeneous suspension, and in the second stage, the resulting suspension is mixed with the extracting liquid in a ratio of 2:1 and mix by shaking the suspension three times at 30 minute intervals between the by shaking for 1.5 hours at a temperature of 2-10°C for 3 days with daily pH correction allergenic extract to 7.0 to 7.2. Used separately, each type of the following frozen synanthropic insects: red cockroach (Blattella germanica), black cockroach (Blatta orientalis), the American cockroach (Periplaneta americana), cockroach ash (marble) (Neuphoeta cinerea), the fly room (Musca domestica), mol a (Tinea pelionella), the bed bug (Cimex lectularis), red ant house (Monomorium pharaonis), the house spider (Tegenaria derhami), the ordinary mosquito (Culex pipiens), Majeed (Attagenus Smirnovi Zhan). The invention is illustrated in the following examples. Example 1. Receive diagnostic allergen of synanthropic insects: red cockroach (Blattella germanica), black cockroach (Blatta orientalis), the American cockroach (Periplaneta americana), cockroach ash (marble) (Neuphoeta cinerea), the fly room (Musca domestica), mol a (Tinea pelionella), the bed bug (Cimex lectularis), red ant house (Monomorium pharaonis), the house spider (Tegenaria derhami), the ordinary mosquito (Culex pipiens), Majeed (Attagenus Smirnovi Zhan), developed in SU NIELS them. Mechnikov RAMS. For diagnostic allergens as feedstock used a mixture of frozen insects of each species separately. A portion of one of these species of synanthropic insects from the freezer transferred into a porcelain mortar and finely cut with scissors to the size of the particles 1 mm Conduct degreasing the crushed material by adding in raw materials ethyl ester in zootoxin and 1:4, keeping raw materials in the air within 15 minutes the Mixture is filtered through a filter paper and left in a fume hood to evaporate the ether. Crushed partially skimmed raw materials in a porcelain mortar, pounding to powder. The resulting powder was subjected to secondary degreasing, which are produced in glass jars with ground stoppers, using 60 grams of raw material 240 ml of ether. Fully skimmed raw materials scattered on enamelled trays with a filter paper and dried in a fume hood to evaporate the ether. The powder is placed in a porcelain mortar and add the liquid for extraction in a 1:1 ratio. Powder pound with the pestle until a homogeneous suspension and transferred to the bottle, which then add another extracting fluid, which comprises sodium chloride, potassium phosphate and sodium at pH 7.0-7.2, the ratio of 2:1. The contents of the bottle gently mix and leave for 30-40 minutes, after which control the pH. The pH correction is performed by adding 1.0 mol/l solution of sodium hydroxide or 0.1 mol/l hydrochloric acid to a pH of 7.0 to 7.2. During the following three days for more complete extraction of protein-polysaccharide complexes carry out the following processing modes: daily bottle of extractable material is placed on the device for shaking the of alcosta in containers three times for 30 minutes with an interval of 1.5 hours. In the intervals between shaking the bottle with the extracted material should be placed in a refrigerated chamber at a temperature of from 2 to 10°C. Daily correction of pH allergenic extract to 7.0 to 7.2. After extraction the supernatant liquid is poured and carry out centrifuged in refrigerated centrifuge at 6000 rpm for 40 minutes followed by filtration through a paper filter. Then hold sterilizing filtration. In day of carrying out this operation it is necessary to control the pH of the allergenic extract. They control the stock solution of the allergen in the same or next day with all the rules of asepsis selected from bottle samples for verification: seeding for sterility, check the pH, which should have a value of 7.0 to 7.2. Visually assess the physical properties of the drug should be transparent, without foreign inclusions, light brown color. The content of protein nitrogen units define method Nessler. A sterile solution of allergen to stabilize the physical and biological properties bear from 1 to 3 months at a temperature of from 2 to 100°C. In cases when the content of units of protein nitrogen in the mother solution of the allergen more than 10,000 PNU/ml, the mother solution of the allergen bred sterile extracting liquid. After R is sliwa allergen receive the finished form of the drug. The resulting preparation was studied taking into account the goals and objectives. The assessment of specific activity of the extract was carried out in an indirect test degranulation of mast cells (DTK) perianalnoe fluid of rats with serum of patients with bronchial asthma, have a history of contact with insects and linking their disease with them. The average of the DCT with the sera of sensitized rats and preparations of allergens from different insects represented in the table.
These data suggest that sensitization with allergens from different species of insects is accompanied by the appearance in the serum of animals ragenovich antibodies that participate in the reactions of immediate type. The structural composition of the obtained preparations of allergens were studied by the method of polyacrylamide gel electrophoresis according to the method of Laemmli. The results of electrophoresis showed that the investigated drugs are a heterogeneous mixture of different proteins. In an electrophoretic separation of allergens various insects was determined by the following fraction: for German cockroach fraction (mol. weighing from 18 to 60 KD; black cockroach 20-35 KD and KD American cockroach before 20cd and more intensely colored 45 KD ash cockroach 20-40 KD and 65 KD fly faction 31 KD mol group fractions from 20 to 40 KD Coheed 20 KD the spider is the most intensely colored faction, 26, 35, 38, 110 KD bug 40-60 KD ant 18, 24 and slightly coloured group with Mm from 45 and above KD. Example 2. Carried out analogously to example 1, except that a portion of one of these species of synanthropic insects from the freezer transferred into a porcelain mortar and finely cut with scissors to the size of the particles 3 mm. Conduct degreasing ISM is Lenogo raw material by adding in raw materials ethyl ester in a ratio of 1:3 and curing of raw materials in the air within 20 minutes Test the diagnostic value of the preparation was carried out according to the program approved by the Committee on medical immunobiological drugs in adults and children 12-15 years in medical institutions of Moscow and Reutov. Studies of substances from different insect species by the method of enzyme-linked immunosorbent assay showed high specific activity. The specific activity of allergens from insects studied in the enzyme-linked immunosorbent assay (ELISA) using conjugate monoclonal anti-Ig E antibodies with peroxidase horseradish root. The study group was 40 atopic patients aged 12 years and older with clinically identified sensitization to the allergen house dust and house dust mites. The results showed that the allergen-specific IgE antibodies to allergens from different insect species were detected in 30% of patients, indicating a high specific activity developed drugs allergens from different species of insects. 1. The method of obtaining diagnostic allergen, comprising the alkaline extraction of raw materials, centrifuging, filtering and sterilizing filtration to obtain the final form of diagnostic allergen, characterized in that the feedstock used the Ute frozen synanthropic insects, crushed raw materials on the particle size of 1-3 mm, degreased crushed raw material by adding in raw materials ethyl ester in a ratio of 1:3-4, sustain raw material in ethyl ether for 15-20 min with subsequent removal of the ether by filtration of the mixture, again degrease raw materials ethyl ether, dried to remove the ether, crushed completely skimmed raw materials to a powder, and extracting the resulting powder is carried out in two stages, the first of which poured the powder extracting liquid 1:1 and stirred until a homogeneous suspension, and in the second stage, the resulting suspension is mixed with the extracting liquid in the ratio 2:1 and mix by shaking the suspension three times at 30 min intervals between shaking for 1.5 h at a temperature of 2-10°C for 3 days with daily pH correction allergenic extract to 7.0 to 7.2. 2. The method according to claim 1, characterized in that use each type of the following frozen synanthropic insects: red cockroach (Blattella germanica), black cockroach (Blatta orientalis), the American cockroach (Periplaneta americana), cockroach ash (marble) (Neuphoeta cinerea), the fly room (Musca domestica), mol a (Tinea pelionella), the bed bug (Cimex lectularis), red ant house (Monomorium pharaonis), the house spider (Tegenaria derhami), the ordinary mosquito (Culex pipiens), Majeed (Attagenus Smirovi Zhan).
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