Method of obtaining shelf fungus ethanolic extract

IPC classes for russian patent Method of obtaining shelf fungus ethanolic extract (RU 2336888):

A61P9/14 - Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers

A61P39/06 - Free radical scavengers or antioxidants

A61P17/02 - for treating wounds, ulcers, burns, scars, keloids, or the like

A61K36/06 - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J0003000000; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L)

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FIELD: medicine, pharmaceutics.

SUBSTANCE: invention concerns the pharmaceutical, food and cosmetic industry. Perform extraction of the raw material crushed with water with obtaining of a water extract and pulp. The pulp, obtained after extraction of raw materials by the water, is extracted in two steps with ethyl alcohol. At the first step of extraction the constrictor is extracted within 4-6 hours using the following proportion: ethyl alcohol 1:(5-7) with concentration of alcohol of 30% either 50%, or 70%, at temperature of 60-75°C, obtaining an ethanolic extract of the first step of extraction and pulp; then, this pulp is repeatedly extracted within 4-6 hours with ethyl alcohol using the following proportion: ethyl alcohol 1:(3-5) with concentration of alcohol of 30% either 50%, or 70%, at temperature of 60-75°C, obtaining an ethanolic extract of the second step of extraction and pulp. After that the ethanolic extract of the first step of extraction is aggregated with the ethanolic extract of the second step of extraction.

EFFECT: increase in active substances content in an extract.

1 ex, 1 tbl

The invention relates to pharmaceutical, food and cosmetic industry and is concerned with the obtaining of alcoholic extract of the fungus. The invention can be used to obtain based medicines, dietary supplements, functional food and cosmetic products with antioxidant, anticarcinogenic, immunomodulatory, regenerating capillaries and mucous membranes, improves cerebral blood circulation, wound healing properties.

A method of obtaining plant polyphenols that possess hepatoprotective effect (patent No. 1510147⁽¹³⁾With MCP⁵AC 35/78, the authors O. Maksimov, krivoshekova PU, Stepanenko PS, Krasovskaya, NP, gruel N, Gorovoy p. g, GEBCO LI, Pokushalov T.V., Saratikov A.S., vengerovsky A.I., Navajeevan SO, primep SO, Vlasova, T., Chuchalin B.C., Gray I.M., patentee: Pacific Institute of Bioorganic chemistry, far Eastern branch of the USSR Academy of Sciences.)

A method of obtaining the amount of flavonoids with radioprotective effect (patent No. 2152794⁽¹³⁾C1 MCP⁷AC 35/78, OR 39/06 author Arkhipova M.N., patentee: Pyatigorsk state pharmaceutical Academy).

The prototype is a method of obtaining funds with anti opisthorchosis action (patent No. 2162701⁽¹³⁾C1 MCP⁷AC 35/78, A61 is 1/16 inventor Burkov V.N., Fights this YEAR, Mozgalina BECAUSE, Chekrygin V.N., patentee: OOO Biolit").

This method is carried out by extraction of the crushed bark of aspen water at 70°With the ratio of components raw water from 1:8 to 1:10, infused over 4-6 hours, drained, defend extract within 2-4 hours, concentrated in vacuo at 30-50°With up to 60 wt.%, the residue is extracted with 45% ethyl alcohol in a ratio of components 1:1 for 4-6 hours, drained, combine the water and alcoholic extracts, followed by evaporation and drying in vacuum.

The disadvantage is the low content of extractive substances, polyphenols, low antioxidant activity of extracts.

The objective is to increase the content of extractive substances, polyphenols, antioxidant activity of extracts.

The task is carried out by extracting the crushed material with water to obtain an aqueous extract and beet pulp, which is extracted with ethyl alcohol, according to the invention the pulp obtained after the extraction of raw materials with water, extracted with ethyl alcohol in two stages, the first stage of extraction, the pulp is extracted for 4-6 hours at a ratio of oil: ethanol 1:(5-7) by alcoholic strength by volume 30%or 50%, or 70%, at a temperature of 60-75°obtaining alcoholic extract of the first stage extraction and pulp, then this is t pulp re-extracted with ethyl alcohol in for 4-6 hours at a ratio of oil: ethanol 1:(3-5) with a concentration of 30%, or 50%, or 70%, at a temperature of 60-75°obtaining alcoholic extract of the second stage of extraction and pulp, after which the alcohol extract of the first stage extraction combined with alcohol extract of the second stage of extraction.

The method is carried out by extracting the crushed material with water to obtain an aqueous extract and pulp. The pulp obtained after the extraction of raw materials with water, extracted with ethyl alcohol in two steps. At the first stage of extraction, the pulp is extracted for 4-6 hours at a ratio of oil: ethanol 1:(5-7) by alcoholic strength by volume 30%or 50%, or 70%, at a temperature of 60-75°obtaining alcoholic extract of the first stage extraction and pulp, then this pulp is re-extracted for 4-6 hours with ethanol at a ratio of oil: ethanol 1:(3-5) with an alcohol concentration of 30%, or 50%, or 70%, at a temperature of 60-75°obtaining alcohol extract the second stage of extraction and pulp. After this alcohol extract of the first stage extraction combined with alcohol extract of the second stage of extraction.

Example

In the extractor is placed 10 g of crushed fungus fungus with a particle diameter of 2-7 mm, the extraction is carried out with water. Get a water extract and pulp. In the extractor is placed 10 g of the oil obtained after extraction chaga water is th, pour 60 ml of ethyl alcohol 30% or 50%, or 70% concentration (the ratio of raw material: alcohol 1:6) and the extraction is carried out in thermostat 5 hours at a temperature of 70°C. thereafter, the alcohol extract is separated from the pulp by filtration, the extract from the first stage extraction and pulp. The pulp obtained in the first stage of extraction, pour a new batch of ethyl alcohol 30% or 50%, or 70% concentration (the ratio of raw material: alcohol 1:4), in the amount of 40 ml, and extracted with 5 hours in a thermostat at a temperature of 70°C. Separating the extract from the pulp by filtration, obtaining the extract from the second stage of extraction. Alcoholic extracts obtained at the first and second stages of extraction, unite.

The content of extractives in ethanol extracts of the fungus, obtained according to the invention, in the above example, presented in table 1. The results repeatedly conducted experiments calculated using Statistics "6", as shown in table 1, when P=0.95, and n=5.

Upon receipt of alcoholic extract according to the invention receive two extract is an aqueous extract, and then from the depleted materials additionally remove extractives ethanol, to obtain an alcohol extract. Therefore, according to the data given in table 1, the content of extractives in the alcohol EXT the acts below in comparison with the previously obtained from this raw water extract. The total solids content extracted from the raw chaga alcohol after the extraction of the raw water, slightly lower than in tinctures obtained industrially. However, total extractives obtained from raw materials in aqueous and alcohol extracts significantly exceed their content in industrial received infusions.

Use extraction with ethyl alcohol in a concentration of 30% or 50%, or 70%, in each case, allows to obtain an extract, which optimally extracted those, or other extractive substances that follows from the data given in table 1. For example, when using a method of obtaining alcoholic extract (for example), using as a solvent ethanol 30 and 70% concentration, the content of total phenolic compounds in the extracts two times higher than in industrialized received infusions. These compounds belong to the class of biologically active compounds. When conducting extraction (for example), when used as a solvent ethanol 50% concentration, it is possible to obtain an extract with a total carbohydrate content at the level of their content in the industrial received infusions.

It should be noted that when using the method of obtaining alcoholic extract of the fungus, extraction with ethyl alcohol (for example) stawinoga raw materials, after extraction with water, allows to extract from raw materials extractive substances with biological activity.

The main active ingredient aqueous extracts of the fungus are polyphenols chagi (polifenoloksikarbonovy complex), which provides therapeutic efficacy of aqueous extracts of the fungus. As shown in table 1, the content in ethanol extracts of the fungus, obtained by the proposed in the invention method (for example), is significant, and their output amounts to 18.2-49.2% of the polyphenol content in water extracts. As can be seen from the table, carrying out extraction for example, 50 and 70% ethyl alcohol can increase the content of polyphenols in 2-3 times in comparison with their content in industrial received the chaga tincture produced from raw chaga without prior extraction chaga water.

The extract obtained by example, when using as a solvent ethanol 50% concentration, has antioxidant activity level obtained with industrial tinctures and aqueous extracts of the fungus.

The use proposed by the invention, a method of obtaining alcoholic extract of the fungus allows its application in an industrial environment for complex processing of raw chaga, obtaining water and alcoholic extracts of CAG and extract more of the extractive substances from raw chaga, to create preventive, medicines and biologically active additives based on them.

The content of extractives in ethanol extracts of chaga
The research object	The alcohol concentration, %	Dry matter, 10 ml, g	Ash substances, 10 ml, g	The amount of carbohydrates, mg/ml	The amount of phenolic compounds, mg/ml	Antioxidant capacity	The output of polyphenols
The mean CL/ml	Sr	Mass, 10 ml, g	%
Obtained by example	30	0,0311±0,00002	0,0022±0,0004	0,35±0,020	0,129±0,0004	-****	-	0,0200±0,0001	2,4
50	0,0364±of 0.00006	0,0004±0,0004	0,b±0,001	to 0.108±0,0004	1,60±0,10	0,05	0,0195±0,0021	6,0
70	0,0323±0,00002	0,0027±0,0004	0,23±0,008	0,122±0,0004	-****	-	0,0172±0,0017	4,6
An aqueous extract of the fungus*	-	0,2182±0,12000	0,0462±0,0400	2,80±0,070	0,470±0,0040	1,80±0,10	0,05	0,1690±0,0041	13,2
Tincture 1**	70	0,0629±0,00060	0,0100±0,0001	0,45±0,008	0,044±0,0001	1,36±0,02	0,01	0,0285±0,0001	-
Tincture 2***	70	0,0533±0,00010	0,0036±0,0001	0,72±0,008	to 0.060±0,0001	2,18±0,08	0,03	0,0278±0,0001	-
Note: * - average defined when P=0.95, and n=30;
*Chaga tincture OJSC Moscow pharmaceutical factory (alcohol);
* - Chaga tincture Phytopharm - NN (alcohol); ** values are specified;.

The method of obtaining alcoholic extract of the fungus by extracting the crushed material with water to obtain an aqueous extract and beet pulp, which is extracted with ethyl alcohol, characterized in that the pulp obtained after the extraction of raw materials with water, extracted Atila the first alcohol in two steps, at the first stage of extraction, the pulp is extracted for 4-6 h at a ratio of oil: ethanol 1:(5-7) by alcoholic strength by volume 30% or 50%, or 70%, at a temperature of 60-75°obtaining alcoholic extract of the first stage extraction and pulp, then this pulp is re-extracted with ethyl alcohol for 4-6 h at a ratio of oil: ethanol 1:(3-5) by alcoholic strength by volume 30% or 50%, or 70%, at a temperature of 60-75°obtaining alcoholic extract of the second stage extraction and pulp, after which the alcohol extract of the first stage extraction combined with alcohol extract of the second stage of extraction.