Method of concentrating and determining chromium and manganese ions in biological substrates |
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IPC classes for russian patent Method of concentrating and determining chromium and manganese ions in biological substrates (RU 2292545):
  
Preparation and method of energization of biological object's vital functions / 2292200
Preparation for energization of biological object's vital functions has carrier processed by alternating magnetic field (AMF). Carrier has to be the matter chosen from the group: pharmaceutical acceptable hard basis which includes salts of single-, two- or three-valent metal or mixture of metals; physiological fluid or liquid crystals. Carrier is processed by AMF by means of frame inductor at magnetic field induction of 1,8-8,0 mcT or by means of solenoid at induction of 1,3-1,5 mcT. The preparation is produced to irradiate within specified AMF induction range during 35-40 minutes. Preparation after being processed is able to keep biological properties from one to a number of years.
Method for predicting scoliotic vertebral column deformity in children and adolescents suffering from oxalate nephropathy / 2291442
Method involves carrying out X-ray examination of the vertebral column and determining CrossLaps content in blood, oxalate calcium crystals available in urine and urine density. man. The value being equal to or less than 45%, external genital endometriosis diagnosis is to be set. Urine density being equal to 1018-1016, oxalate calcium crystals from 1 to 1.5 mg/kg of body mass and CrossLaps value being greater than 0.14 ng/ml, curvature angle is predicted not to be greater than 10°. Urine density being equal to 1015-1010, oxalate calcium crystals content being from 1.6 to 2.0 mg/kg of body mass and CrossLaps value being greater than 0.23 ng/ml, curvature angle is predicted to be from 11°.to 50°. Urine density being less than 1010, oxalate calcium crystals being greater than 2.0 mg/kg of body mass and CrossLaps value being greater than 0.61 ng/ml, curvature angle is predicted not to be greater than 51° with hard secondary transformations being developed.
 Method for predicting efficiency in treating thyroid cysts due to percutaneous ethanol sclerotherapy / 2291430
It is necessary to fulfill a 2-fold thin-needle aspiration biopsy of cystic neoplasms under US control along cytological investigation of aspirate to prove benign nature of cystic neoplasm. For investigation one should take two samples of aspirate: the first - during the first diagnostic puncture, the second - during the second diagnostic puncture; while studying the first sample of aspirate it is important to determine its viscosity; the second sample of aspirate should be studied due to wedge-like dehydration, moreover, one should study the structure of obtained fascia with the help of microscope. High efficiency of treating thyroid cysts due to percutaneous ethanol sclerotherapy could be predicted at the presence of, at least, one of the following signs: 1) viscosity of the first aspirate's sample being under 1.8; 2) availability of inflammation marker - tongue-shaped fields in peripheral area of fascia obtained out of the second aspirate's sample. Application of the present innovation enables to fulfill prediction in shortened terms at simplified technique of implementation.
 Method for predicting corneal subepithelial fibroplasia development after photorefractive excimer laser keratectomy / 2291429
Method involves determining total antitripsin activity in excimer reactive substances concentration in lacrimal fluid concentration in lacrimal fluid before operation. The value being less than 13.2 nmole/min/ml, corneal subepithelial fibroplasia development is to be predicted after the operation.
Method for predicting wound suppuration / 2291428
About 1-3 d after trauma it is necessary to carry out inoculations from wound's lumen and in case of gram-positive sporogenous catalase-forming bacilli one should conclude upon low risk of surgical infection development, and in case of detecting the above-mentioned bacteria in purulent wound - its relatively rapid healing.
 Method for estimating endometrium condition in female genitalia neoplasm cases / 2290639
Method involves taking uterine cavity lavage sample in the amount of 0.2 ml, placing it on object-plate surface as drop and drying. Large cracks predominating in the samples and with gaps between them, benign process is to be diagnosed. The three-radii pattern cracks only being available, malignant process is considered to be the case.
Method for estimating treatment effectiveness in treating latent infantine kron disease cases of ileocecal localization / 2290079
Method involves applying therapy with 5-aminosalicylic acid combine with anti-inflammatory means. Morphopmetric microcirculation bed examination is carried out before and during treatment but not earlier than in half year from the beginning of treatment course with this preparation. The number of blood vessels, microcirculation bed area, nucleoplasmatic ratio of endothelium cells of microcirculation bed and mean blood vessel lumen diameter is determined in ileal mucous membrane bioptate. Sharp angiogenesis reduction being observed as reduced blood vessels number by52±5%, microcirculation bed area reduction by 24±15%, two-fold increase in nucleoplasmatic ratio of endothelium cells of microcirculation bed witnessing their damage reduction and mean blood vessel lumen diameter increase by 40±8% in combination with reduction of perivascular space edema and infiltration, reduction of exudates in tissue, treatment is estimated as effective one.
 Method for predicting the relapse of serous ovarian cancer / 2290078
The present innovation deals with predicting the relapse of serous ovarian cancer after radical therapy before its clinical manifestation. The suggested innovation could be applied in daily practice of pathologoanatomic departments of hospitals, oncological dispensaries, specialized centers and research Institutes. The risk for the development of relapse is represented by a dichotomous variable calculated by the formula determined by logistic regression that includes the combination of several variables being morphological parameters of tumor, that is the area of tumor cell, average ploidy of tumor cells' nuclei, the level of p53 expression the level of expression of estrogen receptors, the level of PCNA expression. The innovation enables to predict repeated cancer growth according to morphological characteristics of primary tumor till clinical manifestation of the relapse.
 Method for predicting gall-stones development in chronic cholecystitis patients on basis of gall chemiluminescence data / 2289814
Method involves studying luminal-dependent iron-induced model system chemiluminescence on citrate-phosphate buffer, determining luminous sum and carrying out repeated model system chemiluminescence registration with chronic cholecystitis patient bile in remission state being added. The luminous sum being grown by 111-127% when compared to model system, stone formation in the gallbladder is to be predicted.
Method for detecting trichomonas forms / 2289813
For detection of trichomonas forms method involves blood seeding in Teras nutrient medium with addition of human or equine serum blood inactivated at temperature +56°C for 5-10 min and antibiotics in the dose 500 U/ml. Culture is incubated for 2 days at temperature +37°C followed by reseeding in similar medium but containing human or equine serum blood inactivated for 15-20 min and antibiotics in the dose 250 U/ml and incubated again at temperature +37°C for 2 days. Then reseeding is carried out in medium containing serum inactivated for 30 min and without addition of antibiotics and samples are incubated at temperature +37° for 2 days. Also, 1-2 additional reseeding are carried out in medium with completely inactivated human or equine serum blood and without addition of antibiotics. Based on active budding or shizogony the conclusion is done about the presence of live atypical forms of trichomonas and in case the absence of multiplication - about the presence of degenerative destructing forms. Using the method allows detecting atypical forms of trichomonas.
 Method of quantitative determination of content of lithium in alloy / 2288289
Proposed method includes determination of initial mass of alloy specimen, heating the alloy specimen under test till separation of free lithium; mass of alloy specimen is determined in inert gas atmosphere; alloy specimen is heated to temperature not below sublimation temperature of pure lithium in vacuum at residual pressure not exceeding 1·10-6 atm; degree of rarefaction in closed space where heating is carried out is checked continuously; abrupt change in angle of inclination of branch of graph of change of specimen mass versus time of extraction of free lithium is indicative of complete distillation of free lithium; quantitative determination of content of free lithium is performed taking into account difference in mass of initial specimen of alloy and mass of specimen recorded at moment of attaining complete extraction of free lithium; specimen of alloy is heated in crucible made from inert refractory material; specimen is loaded into evaporating tube made from inert metal and placed in cavity of evaporating-condensing unit of distillation plant; its inner walls are made from quartz glass. Proposed method may be used for determination of free lithium contained in alloy in chemically unbound state.
Method of assay determination of content of gold in ores and in products of their processing / 2288288
Proposed method includes melting of starting material with lead oxide, soda, borax and flour for obtaining lead alloy (crude lead), its cupellation till gold-silver regulus, dissolving of silver in diluted nitric acid and determination of amount of gold by weighing or by any other instrumental method. Melting process is carried out in metal crucibles at temperature of 600-800°C for 10-30 minutes; charge per 10 g of sample contains the following components: 20-50 g of sodium or potassium hydroxide; 2-20 g of borax; 1-10 g of soda; 15-30 g of litharge and 1-3 g of flour. Method is recommended for assay of samples having mass of 10-100 g.
Method of determination of content of palladium and platinum in ores / 2283356
Proposed method includes decomposition of ore by hydrofluoric and nitric acids followed by further decomposition by aqua regia, boiling-off to moist salts, dissolving of them in hydrochloric acid and extraction. Determination of content of palladium is carried out in organic phase thus obtained and that of platinum is carried out in hydrochloric acid phase. Extractants used for such determination are s-alkylisothiouronium halides and alcohols of C5-C8 fractions, as well as kerosene, benzene, toluene and xylols used as diluents. Used as s-alkylisothiouronium halides are chlorides, bromides and iodides from C7 to C14 and their fractions.
 Method of determination of cause of low impact viscosity of low-carbon steels / 2281975
Proposed method consists in finding-out dependence of grain size of microstructure and presence of ferrite net, as wells as marks on bearing faces of tested specimens for products of the same type made from steel of definite quality during analysis of causes of low impact viscosity recommended standards are established for these parameters and dependences thus found are compared with the data of specimens of low (below standard norm) impact viscosity and these data are estimated for compliance with the recommended standards. Dependences thus found are used repeatedly and constantly.
 Method of a quality control over the test crucible melting / 2272850
The invention is pertaining to nonferrous metallurgy, in particular, to the methods of detection of the noble metals in the mineral raw materials. The technical result of the invention is an increased trustworthiness to the results of the testing crucible melt analysis. The method is conducted in the following way. From the material of the laboratory test sample take out the analytical part of the filler, mix it with the calculated amount of the charge and the mixture is smelt according to the standard method. During the smelt visually control the height of the boiling layer of the melt slag and lead. On completion of the smelt measure the mass of the slag and lead and calculate an admissible height of the boiling layer of the melt according to the following formula:0,9·Hm≥Hc≥[1,9/tg2α/2·(Mш/ρш+Mc/ρc)]1/3, whereHcr - depth of the crucible in meters(m);Hsl - the height of the boiling gas-slag layer, m; α - an angle at the apex of the cone of the inner surface of the crucible, in degrees;Msl, Mla - masses of the slag and mass of the lead alloy accordingly, kg;ρsl, ρla - density of the slag and density of the lead accordingly, kg/m3. If the visual estimation of the height of the boiling layer of the melt exceeds the limits of admissible values, them one may draw a conclusion about the low quality of the testing smelt, make corrections in the composition of the charge and repeat the test analysis.
 Method of preparing samples for analysis / 2267111
Method comprises sampling initial material, producing and analyzing group samples before assessing representative mass of analytical samples, estimating representative mass of analytical samples, and calculating the value of the coefficient that characterizes the type of gold-bearing material from the formula proposed.
 Method for determining hardness limit of austenite class steel / 2265213
Method includes subjecting samples of steel to preliminary plastic deformation and on basis of wear test results of pre-deformed samples, graph of change of hardness limit of σ-1 samples is built dependent on their level of pre-deformation. Weighed samples are made with same deformation level and value of magnetic tear force Pmag is determined for each weighed sample. Graph of change of magnetic tear force Pmag is built for samples on basis of their pre-deformation level, graph with adjusting curve in coordinates Pmag - σ-1, setting a connection between Pmag and σ-1 dependent on level of pre-deformation. Hardness limit of σ-1 samples is determined by adjusting curve in coordinates Pmag - σ-1.
 Method of measuring duration of serviceability of metals / 2261436
Method can be used for estimation of deformation-strength properties due to applying load as well as for determining damages by means of X-ray diffraction analysis. Values of structural-sensitive parameter of crystal lattice of tested material are determined by X-ray diffraction analysis in initial and post-deformation states. Deformation-strength characteristics of metal are determined by calculation from changes in structural-sensitive parameter. Serviceability is judged by comparing really achieved characteristics with admissible ones. Width of X-ray line β is used as structural-sensitive parameters. Strength of deformation P, deformation Δl provided by the deformation and corresponding values of structural-sensitive parameter β are registered during testing. Dependence of true stresses S and structural-sensitive parameter β on degree of relative residual deformation δ are calculated on the basis of P and Δl. Destruction diagram (S-δ½) and linearized diagram (β½-δ½) are built to show inflection points. Deformation-strength characteristics SD and δD corresponding to inflection point at destruction diagram (destruction point D) is taken as criterion of admissible surface strength which provides maximal serviceability of metal. Factor of merit η and factor of destruction Δ can be also taken as criteria of serviceability of metal.
 Mode of testing railway rails on contact weariness / 2253112
Contact weariness is induced by high-frequency dynamic components of interaction of wheels and rails, which become apparent at moving at high speed. The mode of testing railway rails on contact weariness is in that tested samples of rail steel are rolled by pinch rolls in longitudinal direction until appearance on the surface of the sample of dents and also deep indents. As samples test rails are used. The diameter of a pinch roll is chosen under condition of equality of reduction ratio of linear size of the site of contact of the pinch roll with the rail along the axis of the last in comparison with corresponding size responsible to conditions of exploitation and speed reduction ratio of rolling motion of the pinch roll along exploited rail.
Material for production of assay stone / 2248336
Assay stone is made from oxide ceramic comprising BeO-TiO2. Material affords the ability to obtain assay stone of regular geometric form with surface, electrical and mechanical properties meeting the requirement for material used in assaying control. Claimed material in useful in standard determination followed by electrochemical recovery of precision metals from solution after assaying control.
 Method (versions), equipment and system of controlling of automated finding of visualized gas leakage point source / 2292540
Automated finding of location of visualized gas leakage point source is carried out by means of search of angle crf(x*, y*, τ) in response function, which function is calculated on base of row of frames of gas leakage image, of maximal values from variables (x*, y*) being stable in relation to time variable τ. Point (x*, y*) is assumed as point source, if function of response of angle crf(x*, y*,τ) has maximal value at mentioned point (x*, y*) and if this point is time stable.
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FIELD: analytical methods in ecology. SUBSTANCE: method comprises adding test sample to silica gel preliminarily treated with cetylpyridinium chloride and then with phenylfluorene dissolved in water-ethanol medium. Sample is treated at pH 4-5 in determination of chromium and at pH 7-8 in determination of manganese. In order to calculate content of ions, diffuse reflection values are measured on spectrophotometer at wavelengths 530 and 590 nm, respectively for chromium and manganese. EFFECT: achieved 25-fold concentration of Cr(III) and Mn(III) ions and separate determination thereof in case of their common presence. 2 tbl
The invention relates to the field of analytical chemistry and Biomedicine, can be used in ecology for testing and determining ions Cr (III), Mn (II). Known methods for determination of some metals in blood and hair of a man [1-2]. The essence of the method in the atomic-absorption determination of some macro - and microelements after the preliminary sample preparation. While the content of the elementary composition depend on climatic, geochemical and economic conditions of the place of residence. The known method of sorption preconcentration and atomic absorption determination of cobalt, Nickel and cadmium in biosubstrate sorbent polystyrene-oxy-azo-2 oxy-3 nitrobenzol-5'-acid. [3] There is also known a method of physical adsorption of 4-(2-thiazolylazo)-resorcinol, immobilized on the surface of silochrome C-120 (SG-TAR), which is used for the determination of heavy metal ions in the ore, the catalyst with sorption preconcentration and determination by spectroscopy diffusion reflection [4]. All the above methods do not allow us to determine ions Cr (III), Mn (II) because of the low sensitivity and low selectivity, and lack of knowledge in relation to these elements. The task of the invention is the expansion of the range of sorbents by modifying and using novinhas in the analysis of biological objects. The technical result - the creation of an effective solid phases for the concentration and quantitative determination of the elemental composition of biosubstrates and heterogeneous catalysts of a new type. This technical result is achieved by the fact that they get modified sorbent (MS) by immobilization of selective organic reagent - phenylfluorone (FF) to Cr (III) and Mn (II) at different pH on solid media. While immobilized reagent begins to work as a sorbent for preconcentration of trace elements and reagent for testing and evaluation. The essence of the invention is that as the election of the organic reagent used phenylfluorone (FF), dissolved in water-ethanol medium at a ratio of 1:1, immobilized through physical adsorption on a hydrophobic carrier - silicagel CSC No. 2, 5, pre-cleaned and treated with chloride of cetylpyridinium (CPU) and using the differences in pH 4-5 (Cr), 7-8 (Mn), measured the amount of diffuse reflection at wavelengths of 530 and 590 nm, respectively, for Cr (III) and Mn (II). A specific example of implementation The addition of silica gel (SG) in a conical flask and treated with 1 m solution of N2SO4(1 g IG 2 ml H2SO4), then washed with distilled water to a poorly what Isla reaction. Sample of 0.5 g of air-dry mg in conical flasks mixed with reciprocity solution of the CPU, and then after stirring for 30 min enter 0,03% ethanol-water solution FF and create optimal conditions of acidity (pH 4,0-6,0). Prepare standard solutions of ions Cr (III) and Mn (II) with a concentration of 0.1 mg/ml For construction of calibration curve aliquots of standard solutions of the elements were passed through the entire course of the analysis, including sorption on SG-CPU-FF (silica gel-cetylpyridinium-phenylfluorone). In flasks with ground stoppers introduce 0.1 g MS, there also introduce an increasing number of standard solutions of chromium and manganese, create optimal for sorption acidity (pH 4-5 for Cr (III) and pH 7-8 for Mn (II)) using ionomer EV - 74 and shaken on a mechanical vibrator for 60 minutes and Then the adsorbent is filtered through the filter of "blue ribbon" and brought to air-dry state, and then measure the amount of diffuse reflection (R) on spectrophotometer "Spectracron". Calculation of the content of Cr (III) and Mn (II) conduct calibration curve, constructed in advance. The method of determination of heavy metal ions in (a) hair b) blood spectroscopy of diffuse reflection. a) select up to 30 samples of 0.3-0.5 g unpainted human hair with the back of the head, closer to the roots, washed with ether is 4-5 hours, three times changing the ether, then dried to constant weight at a temperature of 60-70°and milled. Hanging hair in the dried state are combined in the overall sample and mix thoroughly. From the total sample sample mass 500 mg is placed in a porcelain Cup, where injected 5 ml of HNO3(conc.) and leave for 10-12 hours. After that, the mixture is heated on a sand bath (t=100° (C) about three hours to get wet salts. Then in the analyzed sample add 2.5 ml of 30% solution of N2O21 ml conc. solution of HClO4and decompose for 20 min at t=150°C, then evaporated almost to dryness. The residue is dissolved in water, bringing the volume up to 10 ml For masking an excess of Al (III) add 0.3 ml of 0.1% solution of NaF. The total volume of the solution was adjusted to 25 ml b) 1 ml of whole blood is transferred into a centrifuge tube, frozen at t=-20°followed by thawing at t=20°C. the Operation of the freezing-thawing was repeated three times. The resulting homogenate was brought to a volume of 10 ml and centrifuged. The precipitate is separated from the solution by decantation. To the precipitate poured 0.5 ml conc. HNO3and heated on a water bath for 1 hour. The mixture is cooled, add 0.25 ml of 0.2% ascorbic acid solution and mixed with the first solution. In the combined solution add 1 ml of 1% solution of glycerol and 7 ml of 1% solution of thiourea and brought to a volume of 25 ml. To alizium samples a) and b) add 0.3 g of modified sorbent SG-CPU-FF, establish optimal adsorption conditions and stirred for 60 minutes. Then the adsorbent is filtered in the filter "blue ribbon", washed 2-3 times with distilled water, dried to air-dry condition and measured values of diffuse reflectance (R) at λ - 530 nm for chromium (III) and λ - 590 nm for manganese (II) on the previously constructed calibration curve. The correctness of the obtained results is tested by the method introduced is found. The results are presented in tables 1, 2.
Thus, the proposed method offers a 25-fold concentration of ions Cr (III) and Mn (II) and their separate quantification in the joint presence. Literature [1] Cysts A.A. Phenomenology of biogeochemistry and biogeographically chemistry. Tashkent: Fan, 1987. 236 C. [2] Avtsin A.P., Havrankova A.A. and other Microelementoses person. M.: Medicine, 1991, 496 S. [3] Basargin, N.N., Queen E.A., etc. Using sorption preconcentration in a study of microelement composition of biosubstrates // Actual problems of analytical chemistry / abstracts. Vol.2. - Moscow, 2002. - P.41-42. [4] Ivanov V.M., Kuznetsov, O.V., Ukr. analyte. chemistry, 1995, CH, No. 5, s-504. The method of concentration and determination of ions of chromium and manganese in biological objects, including add is giving samples to the modified sorbent, establishing optimal for the sorption of acidity, the Department modified sorbents, the measurement of the diffusion reflection on the spectrophotometer, the calculation of the content of ions on the calibration curve, characterized in that the modified sorbent using silica gel pre-treated with chloride of cetylpyridinium, and then phenylfluorone dissolved in water-ethanol medium, the processing of the samples is carried out at pH 4-5 to determine chromium and at pH 7-8 for the determination of manganese, and the values of the diffusion reflection is measured λ = 530 and 590 nm, respectively, for Cr (III) and Mn (II).
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