The way to diagnose european hnilica bees |
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IPC classes for russian patent The way to diagnose european hnilica bees (RU 2122831):
 Method of assessment of air pollution / 2117286
 Installation for testing the flame retardant properties of materials / 2116096
The invention relates to the verification of ognezashita any materials intended for use in means of protection against exposure to high temperatures, heat fluxes, open flame
 A device for determining electrical parameters of fruits and vegetables / 2103679
The invention relates to measurement techniques, in particular to the measurement of electrophysical parameters of fruits and vegetables, and can be used to determine the ripeness of MERCHANTABILITY, fitness for further storage of fruits and vegetables contain nitrates, etc
 The method of determining the quantity of ammonia in the aqueous ammonia solution / 2095809
The invention relates to laboratory testing and can be used in the sugar industry in the preparation of feed water for the diffusion process
 A device for determining oxidizable substances / 2094799
The invention relates to devices for analytical chemistry and is designed for automatic measurement of the content of oxidized products, in particular carbon, lignin substances, sulfite solutions, chemical oxygen demand (COD) of waste, recycling and natural waters, etc
 Method for detection and identification of odors / 2077716
The invention relates to the food, agricultural, and biomedical engineering, in particular to the development of ultrasonic methods for determining the composition of gas mixtures by detecting the corresponding odor substances which can be in any aggregate state: gaseous, solid and liquid
 Forceps-biopsy / 2122354
The method of determining the physiological compatibility of the partnership clients dating services / 2122353
The invention relates to the field of medicine
 Method for the diagnosis of diseases in oncology / 2119669
The invention relates to medicine and can be used in the diagnosis of cancer
A method for diagnosing the presence of the effect of chemical compounds on the body of the intrauterine fetal / 2119300
The invention relates to medicine, in particular to methods of medical examination, and may be used to diagnose the presence of the effect of chemical compounds on the body of the intrauterine fetus by the laboratory without the use of invasive techniques
 Method summary evaluation of the ecological situation in the region / 2118817
The invention relates to medicine and the environment, namely to obstetrics, gynecology and neonatology, and can be used to assess the environmental situation in the region on indicators of reproductive function of women, and also for the diagnosis and prevention of environmentally-dependent pathologies of the reproductive system in women and forecasting the health of the entire population
The method of forming generate the desired gender of the child / 2118125
The invention relates to medicine, and is intended for forming generate the desired gender of the child
 Method for the diagnosis of urogenital chlamydia, mycoplasma and ureaplasmosis / 2117943
The invention relates to medicine, namely to gynecology, andrology, urology, venereology and can be used for diagnosis of such diseases
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(57) Abstract: The method is intended for visual monitoring of a bee colony in the apiary. For this release of sick, dead and healthy larvae of bees and directly in the apiary make the selection of the contents of their intestines. The slide with the corresponding dried smears of samples sent to the laboratory, where they make from strokes seeding and incubated, and then grown in culture analyze under a microscope. This method eliminates the distortion of diagnosis during long-term storage of pathological material and retain the material for further research. The invention relates to veterinary medicine and can be used to diagnose diseases of the brood of bees European grilcom. Known methods of laboratory diagnostics of the European hnilica (Smirnov, A. M. Diagnostics of bacterial diseases of brood // beekeeping. -1984. - N 1. - S. 17-20, N 2. -S. 20-22). Conduct visual monitoring of bee families and prepare pathological material. In a laboratory guide to the honeycomb samples of size 10 x 15 cm, with sick and dead larvae and pupae, in case of death of an unsealed larvae sample dalamanti their selection. Each sample of pathological material treated according to the following scheme: external visual inspection of honeycomb, the selection of patients who died and healthy larvae, opening larvae and selection of the contents of their intestines, preparation of smears of the latest and identification in smears of the causative agent. Diagnosed with European foulbrood can be delivered in 5-7 days by identifying the main causative agent of the disease and concomitant microflora or their associations. The disadvantage of this method is the difficulty of implementation: delivery of material to the laboratory for 1 day is almost impossible, and can lead to mortality as patients and healthy larvae contamination of secondary microflora, mixing the sample with honey. Given that in the sample taken for the study, 100 larvae is 5-10 amazed grilcom, the highlight of larvae among the total mass of the dead is difficult. Often sowing do not diseased and healthy larvae. Even if the seeding produced from larvae affected European grilcom, then highlight the main causative agent using the above method is not possible, because its growth is suppressed by concomitant microflora. Isolated from the mask associated microflora is necessary is o bee colonies. In order to prevent distortion of the diagnosis provided long-term storage of pathological material, to preserve the material for research in the apiary conduct visual monitoring of bee families, there are the patients who died and healthy larvae, select the contents of their intestines. At the same time paying attention to the color of the intestine of the larvae. The intestine is diseased larvae - white /melody/ colorless or yellow heavy, intermittent white inclusions. From the hive to extract cells from diseased larvae. Sterile tweezers remove larvae that reveal sterile eye with a scalpel. Directly in the apiary of the contents of the intestine or decayed mass of dead larvae make strokes on sterile slides three smears from patients who died and clinically healthy larvae. The smear is dried at room temperature in sterile containers, sign them in sterile packaging /plastic bag/ send to the lab. Identification of the causative agent is produced from dried smears out apiary. In the laboratory doing the seeding of stroke on Wednesday Bailey prick and incubated under anaerobic conditions at 35o. After 72 hours appear colony VI is s save to research material, to highlight the main causative agent, to prevent distortion of the diagnosis, since in the dried smear pathogen maintained, and the accompanying microflora dies. The selection of the primary pathogen of the European hnilica gives the opportunity to test its sensitivity to antibiotics, to provide rational treatment. The proposed method allows you to save 10 fotoramok 100 bee colonies and 2 kg of honey per colony. The way to diagnose European hnilica bees, providing visual monitoring of bee families in the apiary, the selection of patients who died and healthy larvae, the selection of the contents of their intestines, preparation of smears of the latest and identification in smears of the causative agent, characterized in that the selection of the gut contents of larvae and subsequent preparation of smears, which are dried, carried out directly after the selection of patients who died and healthy larvae in the apiary, and identification of the causative agent is produced from dried smears out apiary.
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