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Method for electrochemical immunoassay for detecting viruses/viral antigens Invention involves the sandwich-type immune complex formation between antibodies and measles virus/viral antigen followed by attaching an antibody conjugate with a signal-forming label; the signal-forming label represents magnetic nanocomposite particles that are produced by the silicon oxide coating formation on the surface of the magnetic nanoparticles of transition metal oxide, which is supposed to be conjugated with the antibodies; before the stage of the immune complex formation. The immune complex is formed by concentrating it on a solid-phase chemically inert carrier as a result of the magnetic field exposure, which is followed by removing this immune complex from a medium formed on a medium formed on the carrier; the virus/viral antigens and their concentration are detected and measured by a signal generated by transient metal ions formed by the acid treatment of the immune complex. |
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Method of identifying catechol o-methyltransferase modulators Invention relates to medicine and describes method of identifying modulators of enzyme catechol-O-methyltransferase (COMT) activity, including a) obtaining 4-nitrocatechol, covalently bound with Alexa Fluor® 488, b) bringing molecule from stage a) in contact with enzyme catechol-O-methyltransferase (COMT), S-adenosylmethionine (SAM) and candidate compound and c) measurement of indices of fluorescence of mixture from stage b), in which changed indices of fluorescence in presence of candidate compound in comparison with the control serve as sign of presence of modulator of enzyme catechol-O-methyltransferase (COMT). Also described is method of identifying substrate of enzyme catechol-O-methyltransferase (COMT). |
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Method for determining modified nucleotides of rna Selection of pair of oligonucleotide probes suitable for the study area of RNA, selection of a pair of donor and quencher of fluorescence with suitable optical properties is carried out. The direct chemical synthesis of modified probes is carried out, their effectiveness is assessed during melting with RNA containing known modifications. The obtained parameters of melting are assessed for each particular system. The control and the tested RNA are analysed, subjecting the mixture of probes and RNA to intense heating, slow cooling, and then the controlled heating with simultaneous detection of the fluorescence intensity, and the study parameters are selected according to the specific system. The results of melting are analysed, assessing the nature of the curves of melting, and the conclusion is made on the presence or absence of the modification in the test RNA in the presence or absence of modifications in the control RNA. |
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Methods for determining efficacy of ligands of sodium/proton antiporters Invention refers to methods for determining the efficacy of an ion channel ligand. The ex vivo methods for determining the efficacy of the ion channel ligand in vivo depending on plasma, involves the stages as follows: a) contacting a cell expressing the ion channel with i) animal's plasma and ii) the ion channel ligand and b) determining the effect of the ion channel ligand on the cell or a) contacting the cell expressing the ion channel with i) animal's plasma and ii) a compound that is defined as the ion channel ligand, and b) determining the effect of the compound on the cell, or a) contacting the cell expressing the ion channel with animal's plasma wherein the ion channel ligand has been administered, and b) determining the effect of the ion channel ligand on the cell. The method according to the invention may be used for screening of a therapeutic preparation for preventing and/or treating a disease involving the ion channel dysfunction, especially for preventing and/or treating a cardiovascular disease or cancer. |
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Ligands for aggregated molecules of tau-protein Invention relates to method of labelling paired helical filaments (PHF), which includes interaction of PHF with compound and detection of said compound presence, where compound has formula , in which -R- stands for , -Q- is selected from: -NHC(O)-, -N=N-, -CH=CH-; -P is selected from: ; -T is selected from: ; X represents N or CH; -W1-6, -G1-4, -P1-5 are such as given in the invention formula. Invention also relates to method of labelling aggregated tau-protein, which includes interaction of aggregated molecules of tau-protein with compounds and detection of said compound presence, and to compounds of formula , in which values of substituents are such as given in the invention formula. |
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Detection method of proteins in amyloid state, and set for detection of proteins in amyloid state Invention proposes a detection method of proteins in amyloid state, in which a specimen of lysate of yeast culture or tissue of a mammal is obtained, ionic detergent is added to the specimen, proteins are concentrated in an amyloid shape on a cellulose acetate membrane, and they are detected by means of aptomeres, their conjugates or antibodies specific to amyloid shape of proteins. Besides, a set for detection of proteins in amyloid state is proposed. |
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Visualisation agent contains a conjugate of formula (I) of benzopyrylium dye through a linker group with a 3-100-dimensional synthetic peptide which provides directed delivery to the biological target. Also disclosed is a pharmaceutical composition which contains said conjugate of formula (I), a set for preparing said pharmaceutical composition and methods for visualisation of a mammal body in vivo. |
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Fast biosensor with reagent layer Detection system for detecting target molecules includes a sensor chip (1), having on its detecting surface (33) an immobilised target molecule or a capturing molecule for target molecules and a soluble reagent layer (5), having a labelled molecule for binding with the target. The group of inventions also relates to a sensor chip (1) and a method of detecting target molecules in a sample using said sensor chip. |
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Identification of molecules modulating protein-protein interaction Group of inventions refers to methods and systems of analysis based on enzymatic degradation following protein-protein interaction for reporter modulation (activation or inactivation). |
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Method of cell population discrimination and application thereof There is offered a method of discrimination and calculation of at least two populations of biological elements - carriers of specific signs, probably presented in a sample. The method provides the use of three different probes, each of which is specifically fixed with one of the populations of biological elements which are required to be detected. Each probe itself becomes detectable due to its proper marker, and two different markers specified have two emission spectra containing at least one common part (overlapping emission spectra), and the third one has the emission spectrum which essentially contain no common parts with two others (nonoverlapping spectrum). |
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Device and method for detecting flourescent marked biological components Device comprises a measuring cavity for receiving and introducing a fluid sample. The measuring cavity has a set fixed thickness not exceeding 170 micrometres. The measuring cavity has a section fit for acquisition of its image. Within the measuring cavity, there is a dry reagent. The reagent contains as a component, a molecule conjugate with phosphor used for binding with biological components and with all other reacting components. The reacting components are soluble and/or suspended in the fluid sample. The method involves mixing of the reagent with the liquid sample to be introduced in the measuring cavity. A section of the sample in the measuring cavity is exposed to electromagnetic radiation of wavelength corresponding to wavelength of phosphor excitation. Phosphor marked biological components are detected through-thickness of the measuring cavity. Further, numerical analysis of the digital image follows to identify the biological components showing phosphor and to determine amounts of the biological components showing phosphor in the sample. The biological components are discernible on the digital image as fluorescing points emitting electromagnetic radiation of wavelength corresponding wavelength of phosphor emission. |
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Method of multianalytic immune assay with using microparticles Invention refers to biology and medicine, namely to immunodiagnosis. There is offered method of multianalytic immune assay based on immunochemical, genetic and other types of reactions of biospecific binding analyte and ligands. There are mixed various categories of microparticles coated with biospecific reagents for binding of various required analytes and marked with one or more fluorochromes in various concentrations emitting a long-living fluorescence. The analysed sample and biospecific developing reagent marked with a detecting fluorochrome with a short-living fluorescence with its excitation area being outside that of fluorochromes with long-living fluorescence are added to the particle mixture. It is followed with reaction for biospecific complex formation. The prepared biospecific complexes are deposited on a solid-phase carrier. The fluorescence emission of all fluorochromes is excited with emitters in two spectral ranges herewith measuring an amount of long-living fluorescence in a time resolution mode to identify the microparticle and an amount of short-living fluorescence of detecting fluorochrome for measuring concentration of required analytes. Thus the concentration ratio of long fluorescing fluorochromes in microparticles for detecting the same type of analyte is constant, and for determining different types of analytes, the concentration ratio differs at least twice. |
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Method of multyanalite immunoassay with use of microparticles On surface of porous membrane apply the reactionary admixture containing analyte, the first binding molecules bound to detecting substance and specific to analyte, the investigated sample and the particles, not capable to pass through the pores of a membrane covered with the second binding molecules, also specific to analyte, incubate an admixture for formation of a biospecific complex, wash an admixture from not bound reagents and register in a regimen of the time permission phosphorescence signals in spectral ranges of the detecting substances corresponding to a constant of time of attenuation of these substances. Determine the required analyte on a parity of measured phosphorescence signals, thus use on two kinds of the first and second binding molecules, each kind of the first binding molecule is bound to two detecting is long luminescing substances, for example chelate of europium and platinaporphyrine which parity of concentration in each first binding molecule is chosen in advance and corresponds to defined analyte. |
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Method for diagnosing estrogen- and progesteron-dependent genitalia abnormalities Method involves determining estradiol- and progesterone receptors concentration in mononuclear cells fraction of peripheral blood. The value being greater than 210 and 2050 receptors per cell, estrogen- and progesteron-dependent genitalia abnormalities are diagnosed, respectively. |
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Method involves introducing radioisotope to animals and further repeatedly determining radioisotope inclusions percent in blood and in and in non-mineralized organs in given time intervals, calculating relative radio-activity RRA for an animal examined at each time as ratio between radioisotope inclusions percent in non-mineralized organs to radioisotope inclusions percent in blood. Then transport intensity is determined in prevailing directions in each examination time by applying RRA difference factor (DFRRA), calculated as difference between the subsequent and previous RRA values. The received values of DFRRA factor changes are interpreted in terms of intensity time fluctuations and radioisotope transport direction for each organ. |
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Method for detecting toxic action of oral mucosal interferonotherapy One should daily introduce into oral cavity of inbred mouse BALB/c for about 3-5 d an olive-shaped melted edge of capron fish line of 0.5-2.0 mm diameter impregnated with glycerol-containing preparation of recombinant interferon-α, containing 104-106 IU/ml recombinant human interferon-α, then in a dead mouse it is necessary to determine against an intact mouse the morphofunctional state of hepatocytes, enterocytes, lymphoid tissue of regional lymph nodes to evaluate: the absence of toxic action of oral mucosal interferonotherapy in case of no degenerative alterations in hepatocytes, enterocytes, lymphoid tissue of regional lymph nodes in inbred mouse BALB/c subjected to oral mucosal interferonotherapy; the presence of toxic action of oral mucosal interferonotherapy at availability of degenerative alterations of hepatocytes and/or enterocytes, and/or lymphoid tissue of regional lymph nodes in inbred mouse BALB/c after oral mucosal interferonotherapy. The innovation increases information value of the method suggested. |
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Invention relates to method for detection of typical marker foreign DNA sequences, usable in plant modification, in transgenic plant materials and products made of the same. Claimed identification method includes carrying out of multiplex polymerase chain reaction on the base of DNA isolated from tested material followed by hybridization of fluorescent labeled reaction products on specialized bioarrays. Hybridization data are registered by control and program complex and identification of foreign genes and regulatory zones thereof in tested material is carried out, and obtained results are quantitatively interpreted. |
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Method for detecting immunoreactive compounds The present innovation deals with one-stage detection of a bound analyte with a conjugate consisted of an analyte-stereospecific compound (anti-analyte) being covalently conjugated with suspensoid particles of water-insoluble dyestuffs, as coumassi R-250, and/or acridine yellow, and/or acridine orange, and/or 2.4-nitrodiphenyl hydrazine, and/or fluorescein. Application of an anti-analyte with a colored suspensoid label in technology for obtaining conjugates for analysis of one-stage covalent binding leads to considerable simplification of synthesis procedure, increased economy, and reproducibility and higher sensitivity of detection systems. Thus, the innovation enables to improve sensitivity and reliability of stereospecific analysis and optimization of technology to obtain reagents applied in this process being necessary for detection (stereospecific conjugates). |
Another patent 2551365.