Method of individual manufacture of exoprothesis of a nipple-areolar complex
SUBSTANCE: invention relates to medical prostheses and can be used to compensate for tissue deficiency in the region of the remote nipple-areolar complex (NAC) in patients who underwent surgery for the mammary gland. Method includes manufacturing of NAC from silicone. Before the operation to remove the mammary gland, a color scale in tone with the NAC is selected for the patient, a NAC cast is made, the cast is removed and, after complete drying, a two-component soft silicon on a platinum base is poured into its cavity step by step. At the first stage, the nipple cavity is cast and colored in a selected tone. On the second stage, the areolar cavity is filled and colored. Silicone filled mold is dried at room temperature for 30 minutes. Before fixing to the skin, the fixing side of the NAC exoprosthesis is covered with latex and silicone glue.
EFFECT: invention makes it possible to provide a long fixation of the prosthesis to the body and to improve the quality of life of the patient.
1 cl, 10 dwg
SUBSTANCE: invention refers to medicine. What is described is a sterile dehydrated cell-free implant which when rehydrated by water or body fluids is exposed to anisotropic expansion, and can be used as a substrate for living cell adhesion, migration and growth. Collagen structures of a transplant are at least partially denatured after exposure to heat or organic solvents, such as lower aliphatic alcohols and ketones which are simultaneously preserving and sterilising agents, especially with respect to specific types of viruses. The implant is sterilised by radiation, preferentially by accelerated electrons, practically in the dehydrated condition. The transplant can be produced of various animal tissues, especially of mammalian tissues, such as human or swine tissues. Tissues suitable for the prevent invention can represent, e.g. skin, placenta, pericardium, peritoneum, intestinal wall, tendon, blood vessel, etc.
EFFECT: dehydrated implant is suitable as a temporary coating for a wound or a burn, for recovery, replacement and regeneration of tissues, and also as a substrate for cell cultivation in the laboratory conditions, and easier to flex, and being less fragile.
31 cl, 8 dwg, 6 ex
SUBSTANCE: skin equivalent is produced that represents extracellular protein matrix, with mesenchymal cells within it and epidermal cells on its surface. As a matrix collagen or fibrin gel is used. As epidermal cells previously selected keratocyte population with stem cell character is used. Application of the invention allows producing skin equivalent, containing cells in active initial growth stage. Skin equivalent is intended for cutaneous cover regeneration in patients with extensive burns, trophic ulcers, bedsores, and other kinds of wound.
EFFECT: prolonged stimulating effect on patient's cells migration and proliferation.
4 cl, 4 ex
SUBSTANCE: means include biopolymer carrier and cells, applied on carrier mentioned, and characterised as follows: the biopolymer carrier represents collagen-chitosan film, or crumbs of collagen-chitosan film, or high polymer gel in form of 5% macrogol-1500 solution; the cells are diploid human lung strain "ФЭЧ" 16-1 cells, or diploid human musculo-cutaneous tissue strain "ФЭЧ" 16-2 cells in concentration 2.0-3.0×105 to 1 cm2 of film or crumb carrier, or 1.0×106 to 1 ml carrier in form of 5% macrogol solution high polymer gel.
EFFECT: novel means production for replacement therapy based on standard certified cell material, possibility of optimal means form selection, reducing therapy duration, and widening field of application in replacement therapy.
3 cl, 17 ex, 3 tbl
FIELD: medicine, experimental and clinical surgery, combustiology, transplantology, cosmetology.
SUBSTANCE: the present innovation deals with restoring artificial matrix of dermal-epidermal skin equivalent. The method, also, deals with mixing both collagen and chitosan by adding a surface-active substance and a binding agent followed by pouring the mixture obtained, its freezing followed by freeze drying and gamma-sterilization, moreover, before obtaining polyelectrolytic complex of collagen and chitosan one should successively supplement chitosan with aqueous solutions of 1.8 g/g dry chitosan of ascorbic acid, 5-100 mg/g dry chitosan of chondroitin sulfuric acid, 10-100 mg/g dry chitosan of hyaluronic (D-glucuronic) acid, 11-220 mcg/g dry chitosan of cattle serumal growth factor and 2.5-5 mg/g dry chitosan of heparin. The method provides to obtain a structure similar to skin that substitutes skin defect perfectly.
EFFECT: higher efficiency.
6 dwg,1 tbl
SUBSTANCE: wound is prepared. A bioplastic material plate is applied. The bioplastic material is presented by histoequivalent-bioplastic material. This material contains hydrocolloid of a native form of hyaluronic acid and a peptide complex. Viable rounded autogenous tissue flaps are inserted into perforated histoequivalent-bioplastic material. That is covered with a second biomaterial plate.
EFFECT: method enables reducing the length of investing tissue regeneration and provides wound healing with no dressings, protecting from infections and dehydration by using the histoequivalent-bioplastic material, perforating it and applying the autogenous tissue flaps.
2 cl, 13 dwg, 1 tbl, 2 ex
SUBSTANCE: what is described is a biomaterial having a multi-dimensional structure and comprising differentiated MSCs tissue and demineralised bone matrix, wherein the above demineralised bone matrix is dispersed in the differentiated MSCs tissue; a method for preparing and using the same is also presented.
EFFECT: biomaterial is processed as needed and has the mechanical properties required for implantation into the natural involved region.
17 cl, 6 dwg, 2 ex
SUBSTANCE: invention relates to field of medicine, ophthalmology and biotechnology. Claimed is method of preparing cellular structures in form of spheroids for formation of front layers of artificial cornea, which includes application of parts of limbus.
EFFECT: invention can be applied for construction of front layers of artificial cornea in order to compensate damaged tissues of eye cornea.
SUBSTANCE: there are described new reinforced biodegradable frames for soft tissue regeneration; there are also described methods for living tissue support, extension and regeneration, wherein the reinforced biodegradable frame is applied for relieving the symptoms requiring high durability and stability apart from patient's soft tissue regeneration. What is described is using the frames together with cells or tissue explants for soft tissue regeneration in treating medical prolapsed, e.g. rectal or pelvic prolapse, or hernia.
EFFECT: frames are adequately durable to be applicable for implantation accompanying the medical conditions requiring the structural support of the injured tissues.
14 cl, 19 dwg, 2 tbl, 8 ex
SUBSTANCE: invention refers to tissue regeneration by using stem cells and various factors promoting the repair of special tissues and organs stimulating the differentiation of the above endogenous or exogenous stem cells in the cells of the special tissues, thereby recovering a microenvironment of the injured cells.
EFFECT: invention relates to methods, technical devices and compositions for making a framed graft or a transplant in a relatively short time, which can find application in treating or healing injuries and traumas of various human or animal central or peripheral tissues and organs.
10 cl, 11 dwg, 14 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: inventions deal with a membrane, used as a substrate for growing cells of retinal pigment epithelium, its application for supporting cells and a method of inoculating the cells on such a membrane. The characterised membrane is non-biodegradable and porous, covered from at least one side with a glycoprotein-containing coating, with pores with a diameter approximately from 0.2 mcm to 0.5 mcm, with a density of membrane pores constituting approximately from 1×107 to 3×108 pores per 1 cm2, and hydraulic conductivity of the membrane higher than 50×10-10 m sec-1 Pa-1,and having the maximal thickness of 11 mm.
EFFECT: claimed inventions make it possible to obtain a transplant for treatment of age-related macular degeneration.
19 cl, 4 dwg, 6 ex, 3 tbl
SUBSTANCE: group of inventions refers to medicine. What is described is a biological material containing: a) a liquid carrier containing a viscous solution containing at least one natural and/or semisynthetic polysaccharide and having a dynamic viscosity measured at 20°C and at shear rate D=350 s-1, within the range of 100 to 250 centipoise and/or kinematic viscosity within the range of 99 to 248 centistokes (measured in the same environment); b) a autologous or heterologous mesenchymal cell culture and/or c) platelet rich blood product.
EFFECT: material in form of viscous liquid is particularly applicable for the therapy of osteoarthritis, ligament injuries and administered intra-articularly, intradermally or applied in situ without change of the properties of the mesenchymal stem cells and/or its platelets.
11 cl, 5 ex
SUBSTANCE: group of inventions refers to medicine and concerns methods for creation a tooth of a required size, and repair of the lost teeth in the oral cavity by transplantation of the created tooth into the dental loss. Particularly, the method for the tooth creation of a required length in one direction involves the stages: placing the first cell aggregate and the second cell aggregate in a tight contact inside a supporting carrier, wherein the first cell aggregate and the second cell aggregate respectively consists of mesenchymal or epithelial cells; culturing the first and second cell aggregate inside the supporting carrier; the tooth size is corrected by a contact length of the first cell aggregate and the second cell aggregate in the same pre-set direction. A method for determining the contact length of the first and second cell aggregate for the tooth preparation of the required size involves preparing a number of structural types containing structures of various contact length of the first and second cell aggregate in the same pre-set direction; culturing each of a number of structural types inside the supporting carrier; measuring the tooth length prepared at the previous stage in one direction; correlating the given length and contact length providing the basis for calculating the required contact length of the first cell aggregate and the second cell aggregate.
EFFECT: group of inventions enables creating the tooth of the required size that enables preparing a single tooth that can be used as it is in the form of a transplant.
23 cl, 1 tbl, 6 ex, 13 dwg
SUBSTANCE: presented are engineered multilayered vascular tubes, comprising at least one layer of differentiated adult fibroblasts, at least one layer of differentiated adult smooth muscle cells. Further, any layer comprises differentiated adult endothelial cells. The said tubes have the following features: a ratio of endothelial cells to smooth muscle cells makes approximately 1:99 to approximately 45:55; the tube is deformable; an internal diameter of the tube is approximately 6 mm or smaller; the length of the tube is up to approximately 30 cm; the thickness of the tube is substantially uniform along the tube section. It is also provided that the vascular tube is free of any pre-formed frame. What is also described is a method of forming the above tubes.
EFFECT: engineering the therapeutically acceptable alternative vascular tubes withstanding physiological pressure, for transplantation into a patient's body and for use in testing cardiovascular drugs and devices.
29 cl, 4 ex, 3 dwg, 2 tbl
SUBSTANCE: invention refers to a medical prosthesis to be implanted into the human body, particularly to a biological nasal bridge implant used in nasal bridge reparative surgery. The nasal bridge implant is made according to the method which involves sampling, sterilisation and cutting to the required size of the animal material in the form of cattle or swine tendons; cell recovery from the animal material; shaping of the animal material for establishing the required form of the nasal bridge; cross-linking of the animal material; antigen recovery of the animal material, alkaline treatment and introduction of the active substances promoting adhesion of a growth factor and stem cells; packaging of the implant into the container with a sterilisation solution.
EFFECT: preparing the biological nasal bridge implant.
16 cl, 2 dwg, 1 ex
SUBSTANCE: invention refers to medicine, namely to otorhinolaryngology, and can be used in myringoplasty, for repairing partially lost anatomic structures, such as tympanic membrane. The surgery is performed with local or general anaesthesia. A tympanic membrane defect is closed with a thinned prepared alloplant in the form of an allogeneic cartilage plate. Before implanting, the alloplant is fragmented up to 0.2-0.3 mm thick and 0.8-0.9 cm in diameter that is followed by placing the plate into a bottle with a fixing fluid. The final stage of the operation involves placing the alloplant on the edges of the tympanic membrane defect. The cartilage plate is supposed to be more by 1.0-1.5 mm in size with the plate edges to be ovelapped with the acoustic meatus skin. The acoustic meatus is packed.
EFFECT: method provides the reliable fixation of the alloplant, preventing its postoperative dislocation and retraction, audiological characteristics of the alloplant as close to the characteristics of the normal tympanic membrane as possible, the absence of implant rejection and pronounced immune response, proteolytic enzyme stability, necessary rigidity of the cartilage plate, reduced length of the intervention, the absence of a cosmetic auricle defect.
SUBSTANCE: native amniotic membrane is placed into a storage solution containing BSS, gentamicin sulphate, amphotericin B, riboflavin. The amniotic membrane is placed into a sterile container and exposed to ultraviolet light; the ultraviolet exposure is combined with riboflavin instillations. The treated amniotic membrane is placed into the solution containing BSS, gentamicin sulphate, amphotericin B.
EFFECT: increasing the mechanical strength and biological properties of the amniotic membrane for the purpose of increasing the keratoplasty efficacy.
SUBSTANCE: what is described is a method for preparing a bone-cement spacer with an antibiotic consisting in the fact that a spacer surface is coated with coarse-grain salt immersing them completely into the a setting bone-cement layer; after the bone cement has set completely, the spacer is submerged.
EFFECT: more effective delivery of the antibiotic by enlarging the surface area of the cement spacer.
SUBSTANCE: invention refers to medicine and can be used for modifying a surface layer of a three-dimensional products. The ion-plasma processing unit comprises: a working chamber with an ion source; an air lock chamber; a vacuum, heating and cooling shut-off for the working and air lock chambers; a pneumatic system; a control and power supply system, as well as a product positioning system comprising a work table travelling mechanism. The work table comprises vertical spindles with sprockets connected by a closed loop with one of the spindles from the lower side of the work table is provided with the sprocket or a wheel gear, which can roll along a rail fixed on the work table base and comprising a chain or a rack bar respectively, and rotate the vertical spindles of the table a seat holes of which comprise easily-removable locking bolts. Smaller spindles with its pulley rolling along the table surface and rotating with the products mounted thereon about the figure axis inclined to the table surface are attached to the above locking bolts.
EFFECT: invention provides processing the irregular-shaped three-dimensional products for producing the pre-set structure and composition of the near-surface layer of the product.
4 cl, 10 dwg