Method for determination of methyldope concentration in human blood plasma

FIELD: pharmacology.

SUBSTANCE: method for determination of methyldope concentration in the human plasma includes sampling of an aliquot of plasma, its deproteinization, the obtained mixture is centrifuged, methyldope concentration is measured in the supernatant by high-performance liquid chromatography with tandem mass spectrometry in the range from 0.020 to 3 mcg/ml by a calibration graph; the difference is in the application of a deuterated internal methyldope standard solution in methanol for plasma deproteinization, chromatographic separation of the matrix components uses two chromatographic columns Phenomenex Luna Phenyl-Hexyl (50×3.0 mm, 5 mcm) and Phenomenex Synergi Fusion-RP 80A (150×4.6 mm, 4 mcm), and the electrospray ionization is performed simultaneously with chemical ionization at atmospheric pressure.

EFFECT: improved method.

1 ex, 5 tbl



Same patents:

FIELD: medicine.

SUBSTANCE: saliva of affected child is analysed by method of infrared spectroscopy, value of ratio of peak height with maximum at 1070 cm-1 to peak height with maximum 1025 cm-1 is calculated. If value of ratio is in range from 1.1 to 1.9 nonspecific ulcerative colitis is diagnosed, and if value of said ratio is from 2.0 to 4.6 Crohn's disease is diagnosed.

EFFECT: invention ensures less invasive and simpler differential diagnostic of NUC and Crohn's disease in children, ensures simplification and acceleration of diagnosis determination.

4 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to a method of diagnosing bipolar affective disorder. The essence of the method consists in the fact that reliable differences in the spectrum of protein distribution in blood serum without proteins albumin, immunoglobulin G, immunoglobulin A, antitrypsin, transferin and haploglobin in patients with endogenic psychosis. If protein spots are detected on electrophoretic gel in areas with the molecular weight of 200, 84, 75, 49, 40 kDa in the patient with endogenic psychosis, bipolar affective disorder is diagnosed.

EFFECT: application of the claimed method makes it possible to increase accuracy in diagnostics being simple in realisation.

2 ex

FIELD: medicine.

SUBSTANCE: method involves determining female serum cytotoxicity to male lymphocytes, including a combined culture with reference male and analysed female serum in a 96-well tray in the presence of the nutrient medium RPMI 1640 in a CO2 incubator. One day later, lymphocytes are counted in the well in a Goryaev's chamber with the male (reference) and female (analysed) serum. That is followed by determining a cytotoxic index (CI), which represents a quotient of the analysed cell count and the reference cell count. The normal cytotoxic index makes approximately 0.7 and less.

EFFECT: invention enables studying the responses of female humoral immune factors to male antigens and evaluating a risk of miscarriage, early spontaneous abortions and missed miscarriages.

1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to a high-sensitivity method for measuring the amount of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts. The high-sensitivity method for measuring the amount of glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts is characterised by the fact that a mixture of individual's blood plasma with methanol or ammonia water in the specific concentration is introduced into a solid phase having the reverse-phase distribution function and the anion exchange function; the solid phase is then washed with a cleaning fluid that is a single-component fluid or a mixed fluid of at least two components specified in a group containing water, alkali, alcohol and acetonitrile. That is followed by elution from the solid phase in acid alcohol specified in formic acid - methanol or formic acid - ethanol; that is followed by the stage of measuring glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts by liquid chromatography - mass spectrometry or liquid chromatography - mass spectrometry/mass spectrometry.

EFFECT: high-sensitivity method enables detecting and measuring the amounts of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts.

4 dwg, 17 tbl, 7 ex

FIELD: veterinary medicine.

SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.

EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.

2 ex

FIELD: medicine.

SUBSTANCE: patient's peripheral venous blood is recovered to analyse genetic polymorphisms of coagulation factors VII 10976G/A FVII. A birth weight of a newborn of a woman delivering not for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6123.431-25.579x1+0.267x2+205.739x3, wherein y is an anticipated newborn's weight, x1 is a female's height in centimetres; x2 is an infant's weight at the previous delivery in grams, x3 is a genetic version of 10976G/A FVII locus with x3=1 for 10976 GG FVII genetic type, x3=2 for 10976 GA and 10976 AA FVII genetic types. A birth weight of a newborn of a woman delivering for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6278.037-21.739x1+232.170x2, wherein x1 is a female's height in centimetres; x2 is a genetic version of 10976G/A FVII locus with x2=1 for 10976 GG FVII locus, x2=2 for 10976 GA and 10976 AA FVII genetic types.

EFFECT: invention enables improving the prevention and treatment of the pregnancy complications.

2 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: pre-operative fasting venous blood 1 ml is sampled at room temperature 20-24°C into an anticoagulant-free vacuum system (test tube). The test tubes are delivered in a sealed container at temperature 2-8°C for 2 hours to a laboratory for immunoenzyme assay and analysed to determine anti-thyroid stimulating hormone receptor antibodies. If the antibody level is 1.5 units/l or more, a thyroidectomy is performed, whereas the antibody level of less than 1.5 units/l requires performing a subtotal thyroid resection according to standard techniques.

EFFECT: invention enables reducing a probability of recurrent thyrotoxicosis in the patients after subtotal thyroid resection.

1 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to a method for the prediction of a risk of early microvascular complications in the children suffering from type 1 diabetes mellitus. The substance of the method consists in defining a duration of the diseases in years, the patient's age in years, a desquamated endothelial cell count, high-density lipoprotein cholesterol, total cholesterol, triglycerides, atherogenic index, glycohaemoglobin, average daily glycaemic level; making a linear regression analysis and calculating a risk ratio (R) of early microvascular complications in the children suffering from type 1 diabetes mellitus by formula. If the risk ratio is ≥1, the high risk of early microvascular complications during one year is predicted; the ratio < 1 shows the low risk of microvascular complications during one year.

EFFECT: using the declared method enables increasing the prediction accuracy of microvascular complications in the children with type 1 diabetes mellitus.

2 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine and can be used for patient's status monitoring. A method for setting a time-varying physiological parameter warning signal involves patient's controlled parameter monitoring, comparing the controlled parameter to an initial cut-off criterion, varying the cut-off criterion temporarily by a cut-off criterion of deterioration after the therapy, and then after a certain period of time, by the cut-off criterion after the administration. The time allowed involves comparing the controlled parameter to the cut-off criterion of deterioration, and after the time allowed - to the cut-off criterion after the administration. The warning signal is initiated in response to the controlled parameter of one or more initial cut-off criteria, the cut-off criterion of deterioration and the cut-off criterion after the administration. The group of inventions also refers to a machine-readable carrier with software for implementing the method and to a system for user warning on the controlled parameter variation.

EFFECT: group of inventions provides higher accuracy and rate of assessing the patient's status according to the conducted therapy.

15 cl, 6 dwg

FIELD: medicine.

SUBSTANCE: invention can be used for the purpose of the early prediction of cystic periventricular leukomalacia (PVL) in the newborns with very low (VLBW) or extremely low body weight (ELBW). Substance of the method: the newborns with VLBW and ELBW on the 3rd-7th day of life are examined to assess the perinatal medical history, namely the presence of chorioamnionitis and amniotic fluid nature, 5th minute Apgar score, the absence of prolonged artificial pulmonary ventilation, a severity of respiratory distress syndrome, the presence of pneumonia, sepsis, convulsive disorder, anaemia, laboratory signs of the systemic inflammatory reaction, average values of carbon dioxide, anionic bicarbonate and base deficiency in capillary blood, interleukine-6 and receptor interleukine-1 antagonist in venous blood serum. Each sign is assigned with a prognostic coefficient (PC). That is followed by determining total PC, and it is expected cystic PVL that is decided for if total PC is at least (+)9.5, whereas no cystic PVL is expected if total PC is (-)9.5 or less.

EFFECT: invention provides the early (for the first 7 days of life) prediction of cystic periventricular leukomalacia in the most susceptible category of patients that are children with very low and extremely low birth weight.

6 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to investigation and analysis of high-molecular weight materials via infrared spectroscopy when determining the composition of polyacrylate and polyacrylonitrile copolymers to control the quality of carbon fibre. The method includes measuring the infrared absorption spectrum of films of test samples of polyacrylonitrile fibre using Fourier transform infrared spectroscopy in the 3000-800 cm-1 region and determining the content of acrylonitrile and methyl acrylate from normalised spectra based on characteristic peaks thereof. During sample preparation, the number of sample preparation steps is maximally simplified and reduced and dimethyl sulphoxide which does not absorb in the operating infrared region is used. When processing the obtained infrared spectra, the method employs adjustment of the entire base line, smoothing of the shape of the peaks of the investigated compounds and decomposition of the composite peak at 1733±3 cm-1 into components.

EFFECT: invention provides a method for reproducible, precision and sensitive determination of basic components of polyacrylonitrile.

2 cl, 3 tbl, 1 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to genetic engineering and biotechnology. Disclosed is a method of evaluating bioactivity of chemical compounds, where the first step includes transient transfection of cell line HEK 293 with plasmid vector pX-Y-neo (X is any eukaryote transcription factor, Y is a proteotypic peptide corresponding to said transcription factor), which contains a minimal human adenovirus type 5 promoter; a green fluorescent protein gene; a nucleotide sequence which codes the binding site of the transcription factor; a nucleotide sequence which codes the proteotypic peptide; a neomycin resistance gene; the second step includes determining the activity of the transcription factor via fluorescent analysis and chromatographic-mass spectrometer measurement of the content of the proteotypic peptide in the transfected cell culture in the presence of the test substance compared to a transfected intact cell culture.

EFFECT: invention provides fast and highly sensitive evaluation of bioactivity of chemical compounds.

2 dwg, 1 ex

FIELD: agriculture.

SUBSTANCE: device for research of physical-mechanical properties of tuberous roots comprises a frame (1) with an electric motor (2) attached to it, on a shaft of which a removable disc (3) is mounted with the surface under study, and the guide (4) on which a movable trolley (5) is mounted. The movable trolley (5) is connected on one side to the screw mechanism (7) through the spring (6), and on the other side to a load (8) through the block (9). The device is provided with a frequency converter (13) which enables to adjust smoothly the speed of rotation of the removable disc (3), and also a screw mechanism (15) with a guide by which the gap between the trolley (5) and the removable disc (3) is implemented.

EFFECT: increase in the accuracy of the results of research on friction process of rest and movement of tuberous roots on different surfaces.

1 dwg

FIELD: measurement equipment.

SUBSTANCE: invention relates to the field of assessment of atmospheric air pollution degree and may be used in monitoring of atmospheric air in background and urbanised territory. The method provides for identification of a test site with size of 25×25 m, detection of external criteria of lichen on the test site, detection of available indicator types of lichen and frequency of their occurrence. On the basis of the produced data the lichenoindex is calculated, according to the provided classification of lichenoindex, they determine degree of atmospheric air pollution.

EFFECT: invention makes it possible to detect degree of atmospheric air pollution by lichen.

4 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: object is positioned on porous substrate, fixed to the substrate surface and scanned by probe microscopy method. Substrate with through pores of smaller size than the diameter of a study object is used, and an object is fixated by laminar flow of liquid or gas supplied to the substrate from the side of scanning, with clamping force exerted by the flow on an object within 10-12-10-3 N range.

EFFECT: possible study of structures and mechanical properties of organic and inorganic objects, enhanced information content of nano and micro object studies by probe microscopy.

7 ex

FIELD: agriculture.

SUBSTANCE: invention relates to ecology. The invention provides a method of determining the quality of the environment by EPR-spectroscopy of lichens, including the collection of samples of lichen thalli from the trunks of trees growing in an industrial and background area, which is not contaminated by anthropogenic emissions into the environment, cleaning, drying, grinding, which is characterised by the fact, that drying is carried out at a temperature of 85-95°C to constant weight and ground, the EPR spectra are removed, from which the concentration of paramagnetic centres is determined, in the excess of the concentration of the paramagnetic centres in the lichen samples collected in the industrial zone, over the concentration of the paramagnetic centres of the lichen samples from the background area the low quality of the environment in the industrial zone is detected, and in case of equal concentrations of the paramagnetic centres - the acceptable quality of the environment is detected, and in the studies the samples of the same species of lichen are used.

EFFECT: invention provides the improvement of the method of lichenoindication, improvement of the quality of evaluation of the test objects, obtaining objective result.

2 ex, 2 tbl, 3 dwg

FIELD: ecology.

SUBSTANCE: method of determining ammonium compounds in the atmosphere of livestock complexes comprises collecting samples of lichen from trees growing in the background zone, which has no emissions of pollutants into the atmosphere. The data for the samples of lichen collected in the zone of pollutants emission to the atmosphere is compared with data for laboratory standards by IR spectroscopy method. For obtaining the standards under laboratory conditions the interaction process of lichen of the background zone with emission of pollutants contributing to formation of ammonium sulphate is simulated. Lichen Parmelia sulcata is used as bioindicator.

EFFECT: invention enables to determine the level of ammonium compounds in the atmosphere of livestock complexes.

2 tbl, 1 dwg

FIELD: metallurgy.

SUBSTANCE: invention relates to ferrous metallurgy and can be used to determine chemical composition of materials containing lump metal and used as raw material in cast iron production. The method involves separation of material into metal and slag fractions, measurement of metal fraction weight, grinding of slag fraction down to the fineness of 5 mm at most and determination of weight ratio of total ferrum and of necessary components in it by complete acid digestion, calculation of weight ratio of total ferrum and of components in the material, after grinding a sample is taken with the fineness of from 0.16 mm to 5 mm at most and chemical analysis is performed.

EFFECT: improved information value and reliability of analysis.

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used for the prediction of the early stage of lymphocyte apoptosis. That is ensured by isolating cells, incubating for 48 hours at temperature 37°C with 5% CO2 with adding apoptosis inductor, dexamethasone in the concentration of 10-4 mole/ml. A lymphocyte viability is quantified by trypan blue inclusion, the recovered and oxidised glutathione concentrations are measured in lymphocyte lysate after the 30-minute pre-incubation with 2-vinylpyridine 10 mM. The early stage of lymphocyte apoptosis is stated, if observing an integrated decrease of the recovered glutathione concentration by 17% and more and an increase of the oxidised glutathione concentration by 19% and more as compared to the reference.

EFFECT: using the presented method in medical practice enables predicting the antioxidant state of the patient's body accompanying various diseases as shown by the early stage of lymphocyte apoptosis evaluated.

1 tbl

FIELD: chemistry.

SUBSTANCE: method includes the selection and preparation of samples to be analysed, selection of specified volumes of solutions of a test system components, placement of the samples to be analysed and the test system components into a cuvette, registration of chemiluminiscence with further quantitative estimation of its value with taking into account the background signal of chemiluminiscence. The weight of porting of the sample of the material to be analysed is taken such that corresponds to the value of a specific surface 0.20±0.05 m2/g, and in case when it is not possible to determine the value of the specific surface of the sample to be tested, the weight of the taken portion is 0.010±0.005 g. The portion of the sample of the material to be analysed is placed into a cuvette with the further successive addition of the test system components: 0.01M solution of luminal in 0.5 NaOH solution and a solution of hydrogen peroxide of a 20-30% concentration to fill the working space in the cuvette, keeping the ratio luminal:hydrogen peroxide equal to 2:5. After that, values of chemiluminiscence are registered for 125 minutes and the total value of chemiluminiscence is calculated.

EFFECT: identification of the free-radical activity of solid materials by the method of the chemiluminiscence registration by means of the system of chemical reagents without the application of biological substrates in the test system.

3 tbl, 3 ex

FIELD: technologies for testing properties of materials.

SUBSTANCE: method for determining composition of bi-nonary condensed type systems, in case of core sizes in fractions d1>d2>d3>…>d9>d10 and in case of core dimensions relations d2/d1,d3/d2,…,d10/d9 greater than 0.155 fractions volumes are determined from formulas for binary systems V1=1m3, Y1=1-d2/d1, V2=1m3·Y1·Ve1,m3, for ternary systems Vsm2=1m3, Y2=1-d3/d2av, V3=1m3·Y2·Vemp,m3, for quaternary systems Vsm3=1m3, Y3=1-d4/d3av, V4=1m3·Y3·Vemp3,m3, for quinary systems Vsm4=1m3, Y4=1-d5/d4av, V5=1m3·Y4·Vemp4,m3, for senary systems Vsm5=1m3, Y5=1-d6/d5av, V6=1m3·Y5·Vemp5,m, for septenary systems Vsm6=1m3, Y6=1-d7/d6av, V7=1m3·Y6·Vemp6,m3, for octuple systems Vsm7=1m3, Y7=1-d8/d7av, V8=1m3·Y7·Vemp7,m3, for nonary systems Vsm8=1m3, Y8=1-d9/d8av, V9=1m3·Y8·Vemp8,m3, where V1,V2…,V9 - piled up fraction volume with core sizes respectively d1,d2,…,d9,m3,Vsm2,Vsm3,…,Vsm8 - piled up volume of binary, ternary,…,octuple friable condensed type system, m3, Y1,Y2,…,Y8 - coefficient of condensation level of fraction with core size d1 by fraction with core size d2, condensation of binary friable system with average core size d2av by fraction with core size d3,…, condensations of octuple friable systems with average core size d8av by fraction with core size d9, Ve1 - emptiness of fraction with core size d1, Vemt2, Vemt3,…,Vemt8 - emptiness value for binary, ternary,…, octuple friable condensed type system.

EFFECT: lower laboriousness, higher effectiveness, possible optimization by utilizing computer means.

11 ex, 1 tbl