Peptides suppressing respiratory viruse infections, their application and methods for obtaining

G01N2333/11 - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (separating components of materials in general B01D, B01J, B03, B07; apparatus fully provided for in a single other subclass, see the relevant subclass, e.g. B01L; measuring or testing processes other than immunoassay, involving enzymes or micro-organisms C12M, C12Q; investigation of foundation soil in situE02D0001000000; monitoring or diagnostic devices for exhaust-gas treatment apparatus F01N0011000000; sensing humidity changes for compensating measurements of other variables or for compensating readings of instruments for variations in humidity, seeG01D; or the relevant subclass for the variable measuredtesting or determining the properties of structures G01M; measuring or investigating electric or magnetic properties of materials G01R; systems in general for determining distance, velocity or presence by use of propagation effects, e.g. Doppler effect, propagation time, of reflected or reradiated radio waves, analogous arrangements using other waves G01S; determining sensitivity, graininess, or density of photographic materials G03C0005020000; testing component parts of nuclear reactors G21C0017000000)

FIELD: pharmacology.

SUBSTANCE: inventions relate to a peptide synthesized chemically or genetically engineered, compositions comprising such a peptide, DNA coding a polypeptide, vector incorporating such a DNA, a host cell for expression of the peptide, a peptide screening Kit, capable of suppressing a respiratory virus infection and a method for screening of a peptide capable of suppressing a respiratory virus infection. The presented peptide contains 5 or more essential amino acids, 2 or more of these essential amino acids are located in the N-terminal or C-terminal region, and the N-terminal region contains a sequence of no more than 10 amino acids from the peptide N-terminal amino acid and the C-terminal region contains a sequence of no more than 10 amino acids from the peptide C-terminal amino acid, while the peptide consists of a sequence of amino acids, at least 90% identical to SEQ ID NO: 10.

EFFECT: possibility of application of inventions to block infections of respiratory viruses such as influenza viruses or coronaviruses in the target cells for prevention and treatment of these infections.

23 cl, 10 dwg, 3 tbl, 1 ex



Same patents:

FIELD: chemistry.

SUBSTANCE: invention relates to investigation and analysis of high-molecular weight materials via infrared spectroscopy when determining the composition of polyacrylate and polyacrylonitrile copolymers to control the quality of carbon fibre. The method includes measuring the infrared absorption spectrum of films of test samples of polyacrylonitrile fibre using Fourier transform infrared spectroscopy in the 3000-800 cm-1 region and determining the content of acrylonitrile and methyl acrylate from normalised spectra based on characteristic peaks thereof. During sample preparation, the number of sample preparation steps is maximally simplified and reduced and dimethyl sulphoxide which does not absorb in the operating infrared region is used. When processing the obtained infrared spectra, the method employs adjustment of the entire base line, smoothing of the shape of the peaks of the investigated compounds and decomposition of the composite peak at 1733±3 cm-1 into components.

EFFECT: invention provides a method for reproducible, precision and sensitive determination of basic components of polyacrylonitrile.

2 cl, 3 tbl, 1 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to genetic engineering and biotechnology. Disclosed is a method of evaluating bioactivity of chemical compounds, where the first step includes transient transfection of cell line HEK 293 with plasmid vector pX-Y-neo (X is any eukaryote transcription factor, Y is a proteotypic peptide corresponding to said transcription factor), which contains a minimal human adenovirus type 5 promoter; a green fluorescent protein gene; a nucleotide sequence which codes the binding site of the transcription factor; a nucleotide sequence which codes the proteotypic peptide; a neomycin resistance gene; the second step includes determining the activity of the transcription factor via fluorescent analysis and chromatographic-mass spectrometer measurement of the content of the proteotypic peptide in the transfected cell culture in the presence of the test substance compared to a transfected intact cell culture.

EFFECT: invention provides fast and highly sensitive evaluation of bioactivity of chemical compounds.

2 dwg, 1 ex

FIELD: agriculture.

SUBSTANCE: device for research of physical-mechanical properties of tuberous roots comprises a frame (1) with an electric motor (2) attached to it, on a shaft of which a removable disc (3) is mounted with the surface under study, and the guide (4) on which a movable trolley (5) is mounted. The movable trolley (5) is connected on one side to the screw mechanism (7) through the spring (6), and on the other side to a load (8) through the block (9). The device is provided with a frequency converter (13) which enables to adjust smoothly the speed of rotation of the removable disc (3), and also a screw mechanism (15) with a guide by which the gap between the trolley (5) and the removable disc (3) is implemented.

EFFECT: increase in the accuracy of the results of research on friction process of rest and movement of tuberous roots on different surfaces.

1 dwg

FIELD: measurement equipment.

SUBSTANCE: invention relates to the field of assessment of atmospheric air pollution degree and may be used in monitoring of atmospheric air in background and urbanised territory. The method provides for identification of a test site with size of 25×25 m, detection of external criteria of lichen on the test site, detection of available indicator types of lichen and frequency of their occurrence. On the basis of the produced data the lichenoindex is calculated, according to the provided classification of lichenoindex, they determine degree of atmospheric air pollution.

EFFECT: invention makes it possible to detect degree of atmospheric air pollution by lichen.

4 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: object is positioned on porous substrate, fixed to the substrate surface and scanned by probe microscopy method. Substrate with through pores of smaller size than the diameter of a study object is used, and an object is fixated by laminar flow of liquid or gas supplied to the substrate from the side of scanning, with clamping force exerted by the flow on an object within 10-12-10-3 N range.

EFFECT: possible study of structures and mechanical properties of organic and inorganic objects, enhanced information content of nano and micro object studies by probe microscopy.

7 ex

FIELD: agriculture.

SUBSTANCE: invention relates to ecology. The invention provides a method of determining the quality of the environment by EPR-spectroscopy of lichens, including the collection of samples of lichen thalli from the trunks of trees growing in an industrial and background area, which is not contaminated by anthropogenic emissions into the environment, cleaning, drying, grinding, which is characterised by the fact, that drying is carried out at a temperature of 85-95°C to constant weight and ground, the EPR spectra are removed, from which the concentration of paramagnetic centres is determined, in the excess of the concentration of the paramagnetic centres in the lichen samples collected in the industrial zone, over the concentration of the paramagnetic centres of the lichen samples from the background area the low quality of the environment in the industrial zone is detected, and in case of equal concentrations of the paramagnetic centres - the acceptable quality of the environment is detected, and in the studies the samples of the same species of lichen are used.

EFFECT: invention provides the improvement of the method of lichenoindication, improvement of the quality of evaluation of the test objects, obtaining objective result.

2 ex, 2 tbl, 3 dwg

FIELD: ecology.

SUBSTANCE: method of determining ammonium compounds in the atmosphere of livestock complexes comprises collecting samples of lichen from trees growing in the background zone, which has no emissions of pollutants into the atmosphere. The data for the samples of lichen collected in the zone of pollutants emission to the atmosphere is compared with data for laboratory standards by IR spectroscopy method. For obtaining the standards under laboratory conditions the interaction process of lichen of the background zone with emission of pollutants contributing to formation of ammonium sulphate is simulated. Lichen Parmelia sulcata is used as bioindicator.

EFFECT: invention enables to determine the level of ammonium compounds in the atmosphere of livestock complexes.

2 tbl, 1 dwg

FIELD: metallurgy.

SUBSTANCE: invention relates to ferrous metallurgy and can be used to determine chemical composition of materials containing lump metal and used as raw material in cast iron production. The method involves separation of material into metal and slag fractions, measurement of metal fraction weight, grinding of slag fraction down to the fineness of 5 mm at most and determination of weight ratio of total ferrum and of necessary components in it by complete acid digestion, calculation of weight ratio of total ferrum and of components in the material, after grinding a sample is taken with the fineness of from 0.16 mm to 5 mm at most and chemical analysis is performed.

EFFECT: improved information value and reliability of analysis.

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used for the prediction of the early stage of lymphocyte apoptosis. That is ensured by isolating cells, incubating for 48 hours at temperature 37°C with 5% CO2 with adding apoptosis inductor, dexamethasone in the concentration of 10-4 mole/ml. A lymphocyte viability is quantified by trypan blue inclusion, the recovered and oxidised glutathione concentrations are measured in lymphocyte lysate after the 30-minute pre-incubation with 2-vinylpyridine 10 mM. The early stage of lymphocyte apoptosis is stated, if observing an integrated decrease of the recovered glutathione concentration by 17% and more and an increase of the oxidised glutathione concentration by 19% and more as compared to the reference.

EFFECT: using the presented method in medical practice enables predicting the antioxidant state of the patient's body accompanying various diseases as shown by the early stage of lymphocyte apoptosis evaluated.

1 tbl

FIELD: chemistry.

SUBSTANCE: method includes the selection and preparation of samples to be analysed, selection of specified volumes of solutions of a test system components, placement of the samples to be analysed and the test system components into a cuvette, registration of chemiluminiscence with further quantitative estimation of its value with taking into account the background signal of chemiluminiscence. The weight of porting of the sample of the material to be analysed is taken such that corresponds to the value of a specific surface 0.20±0.05 m2/g, and in case when it is not possible to determine the value of the specific surface of the sample to be tested, the weight of the taken portion is 0.010±0.005 g. The portion of the sample of the material to be analysed is placed into a cuvette with the further successive addition of the test system components: 0.01M solution of luminal in 0.5 NaOH solution and a solution of hydrogen peroxide of a 20-30% concentration to fill the working space in the cuvette, keeping the ratio luminal:hydrogen peroxide equal to 2:5. After that, values of chemiluminiscence are registered for 125 minutes and the total value of chemiluminiscence is calculated.

EFFECT: identification of the free-radical activity of solid materials by the method of the chemiluminiscence registration by means of the system of chemical reagents without the application of biological substrates in the test system.

3 tbl, 3 ex

FIELD: veterinary medicine.

SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.

EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.

2 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine and biology, namely to a method for additional electron-dense contrast enhancement of acid groups of biomolecules accompanying the histochemical detection of sodium cations in pulmonary and tracheal cell and tissue ultrastructures. Substance of the method consists in fixing tissue slices, washing the surface in bidistilled water, placing into a diluted agent containing 4% osmium tetraoxide 1 ml and 2% potassium hexahydroantimonate 8 ml. The tissue slices are stained for 4 hours while stirring strongly and washed in bidistilled water. The tissue slices are further prepared by slicing thinner and dehydrated; the semi-thin and ultrathin slices are produced and studied by a transmission electron microscope, which is followed by computer processing to detect diffuse selective staining of the acid cell ultrastructures and intracellular substance.

EFFECT: using the declared method enables the additional electron-dense contrast enhancement of the acid groups of biomolecules accompanying the histochemical detection of sodium cations in the pulmonary and tracheal cell and tissue ultrastructures.

5 dwg, 1 ex

FIELD: electricity.

SUBSTANCE: preparation method of a dielectric specimen for investigation on a focused-beam electronic microscope of its micro- and nanostructure involves application of a current-carrying coating onto the specimen surface and provision of electrical contact of the specimen coating with the current-carrying object table. The current-carrying coating is applied by wetting of the specimen surface with a solution of hydrophilic non-evaporable non-flammable non-toxic current-carrying ionic liquid in the form of tetrachloroferrate of N-decylpyridinium in acetone and further drying of the specimen in the air till complete removal of a volatile component.

EFFECT: prevention of accumulation of electrical charges on the surface of dielectric specimens.

2 dwg

Sampling device // 2556851

FIELD: oil and gas industry.

SUBSTANCE: sampling device contains a main pipeline, a sampling section joined with the main pipeline with a possibility of sampling with coverage of liquid flow cross section, a sampling tap and a manometer. The sampling tap is designed as housing and bushing which is rigidly joined with a handle, and in a starting position the bushing shuts a drain hole of the housing, and the handle can move together with the bushing, opening the drain hole of the housing in working position. The hollow cylinder with the central channel is installed in the main pipeline, and the hollow cylinder from side of liquid flow movement is fitted with the input cone tapering the flow and the output cone expanding the flow on the other side of the hollow cylinder. The whirler fitted inside with tangential channels is installed in the central channel of the hollow cylinder from the input cone. The bushing of the sampling tap covers hermetically from outside the housing with the drain hole and can move restrictedly in axial direction with reference to the housing. The housing has the first and second external cylindrical grooves and the cutting spring lock ring is located inside. The bushing is fitted with internal ring sampler.

EFFECT: improvement of quality of liquid sampling, improvement of operational reliability of the sampling tap and increase of level of correctness of identified process parameters of wells and layers according to analyses of samplings.

2 dwg

FIELD: biotechnology.

SUBSTANCE: method comprises the steps: collecting from the bile ducts of liver of domestic and/or wild animals infected with fasciolas of only live adult F. hepatica. Placement them into individual tubes with filtered and centrifuged bile diluted with isotonic solution of sodium chloride 1:1. Exposure of tubes at t = 38-39°C if F. hepatica is from cattle, and t = 39-40°C if F. hepatica is from sheep and/or goats, under conditions of thermostat for 5 hours. Subsequent washing eggs in isotonic solution of sodium chloride.

EFFECT: invention enables to obtain up to 100 percent of fertilised eggs of Fasciola of species Fasciola hepatica and can be used for study in the laboratory or field experiments in solving fundamental and applied scientific tasks in the field of epizootiology, treatment and prevention of fascioliasis of domestic or wild animals.

2 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agricultural machinery industry. The device for sampling chopped straw from grain combine harvesters comprises collectors, a retainer, a bracket and a control lever. The collectors are arranged in rows, the number of which in the transverse direction is determined by the header coverage and the selected number of the studied areas, and in the longitudinal - replication of sampling. The collectors are interconnected in longitudinal rows by means of flexible connections equal in magnitude, so that the total length of the row does not exceed the distance to the ground mass descent area on the stubble. The first collectors in each longitudinal row have easily removed connections on the holes in the rod-float leveller which is located perpendicular to the direction of the combine movement and retained by the retainer driven by the control lever.

EFFECT: invention provides a boundary separation of the comparable areas in sampling and reduction of the probability of failure of experimental equipment.

2 cl, 1 dwg

FIELD: instrumentation.

SUBSTANCE: invention relates to the method of analysis of a set of ferromagnetic particles. The method is characterized by that the particles of the named set are levelled in such a way that each of the named particles is oriented practically in the same direction. Then the particles of the named set are fixed in this aligned direction and the internal areas of the named particles levelled in such a way are uncovered. After that the nature of the alloy comprised by each of the named particles is identified, the named particles are grouped by categories depending on their nature and the metallurgical structure and chemical composition of one or more of the named particles in each category are determined.

EFFECT: improvement of accuracy and reliability of the analysis of ferromagnetic particles.

14 cl, 6 dwg

FIELD: instrumentation.

SUBSTANCE: invention relates to forecast of ageing processes of the synthetic polymer materials (SPM) depending on duration of their operation or storage. Analysis of the volatile organic compounds (VOC) migrating from SPM is performed by active sampling for sorbent, with further thermal desorption and gas chromatographic analysis. Forecast of the ageing processes of the material and estimation of toxicity of the gas discharge are performed as per dynamic of the qualitative and quantitative composition of gas discharge components in SPM initial state, and during artificial climatic thermal-humidity ageing. Dynamics analysis of the total gas discharge (ΣT) from each material is performed for all substances migrating from studied SPMs. Change of toxicity is estimated and forecast of the material ageing is performed as per developed indices of total gas discharge (ΣT) and as per hygienic index P=(ΣTinitial/ΣTn)/V, where Tinitial and Tn are indices of toxicity of gas discharge of each substance in initial state and after ageing, respectively, and ΣTinitial and ΣTn are total indices of toxicity of gas discharge of all components of SPM in initial state and after ageing, V is duration of ageing (year, month).

EFFECT: invention ensures high accuracy of the method of VOC qualitative and quantitative composition determination in gas discharge during materials ageing and the analysis results repeatability.

3 tbl

FIELD: physics, acoustics.

SUBSTANCE: group of inventions relates to an apparatus for irradiating a sample with focused acoustic energy, a device which is part of said apparatus, a cartridge for said device and a method of irradiating a sample with focused acoustic energy. The apparatus comprises a device, a cartridge, a completely solid-state connector and a source for generating acoustic energy. The cartridge has a chamber for receiving a sample, and the completely solid-state connector provides a completely dry coupling of acoustic energy between the source and the cartridge. The device and the cartridge are adapted for inserting the cartridge containing a sample into the device and are separable, and the focused acoustic energy is focused high-intensity ultrasound. The device has a source for generating acoustic energy and the cartridge has a chamber for receiving a sample.

EFFECT: improved sample processing.

17 cl, 24 dwg

FIELD: motors and pumps.

SUBSTANCE: simulation device contains an oil batcher, a dispersion chamber and a lubricant oil decomposition chamber (1). At the air outlet downstream the chamber the diffuser (2) is located. On the chamber the heater (3) with the thermocouple (4) and the thermorelay (5) is installed. The device includes the air duct (6) supplying the pumped-over hot air into the lubricant oil decomposition chamber connected through the manometer (7) to the air compressor (8). The device contains the cylinder (13) filled with ultra-pure nitrogen, (23, 24) the sealed gage tank with air cavity with oil and a cover for oil filling, with the oil pipeline connected to it through the gas pipeline with the regulator (12), the adapter (11) and cap nuts. The gage tank (9) is connected through the adapter (11) with cap nuts (20, 21) to the measured capillary (15) in a cooling jacket (16) with circulating water through the thermostat with the pump (18) and radiators, attached to the decomposition chamber by means of the cap nut (22) and the sealing cone (25). Also the device comprises the additional chamber (26) screwed to the main decomposition chamber of (1) coaxially and sealed with a gasket (27), with the rod with a flywheel (17) installed inside, with threaded and non-threaded parts. Meanwhile the threaded part is implemented with a possibility of movement in the internal washer with a thread (28) for adjustment of the decomposition chamber volume and change of conditions of simulation of oil concentration, while the non-threaded part is sealed in a gland with graphite seal (29).

EFFECT: improvement of accuracy of simulation of composition of oil decomposition products in aviation gas-turbine engines.

1 dwg

FIELD: medicine.

SUBSTANCE: claimed invention relates to the field of biotechnology. Claimed are versions of a humanised anti-CD79b antibody, each of which is characterised by the presence of a light and heavy chain and a set of 6 CDR with a determined amino acid sequence. An epitope of the antibody from 11 amino acids is determined by the Biacore method. Disclosed are: an immunoconjugate of the antibody with a medication or means for inhibiting cell growth, where the antibody is bound with means covalently, and versions of the composition, based on an effective quantity of the immunoconjugate or the antibody, used for inhibiting B-cell proliferation; as well as a method of determining CD79b in a sample with the application of the antibody. Described are: an antibody-coding polynucleotide, as well as an expression vector and an isolated cell, containing the vector for obtaining the antibody. Disclosed are versions of applying the antibody or immunoconjugate for obtaining the medication for inhibiting the growth of CD79b-expressing cells for the treatment of an individual, affected with cancer, for the treatment of proliferative disease or for inhibiting B-cell proliferation.

EFFECT: invention provides novel antibodies, which can find further application in the therapy of proliferative CD79b-associated diseases.

91 cl, 8 tbl, 9 ex, 20 dwg