Method of producing coordination compounds of oligogalacturonic acid with biogenic metals (ii) as delivery systems of biogenic metals (ii) and systems for removing heavy metals (ii)

FIELD: medicine.

SUBSTANCE: invention relates to pharmacology, and describes a method of producing coordination compounds of oligogalacturonic acid with biogenic metals (II). Method involves production of pectin-containing preparation of oligogalacturonic acid with polymerisation degree equal to no less than 10, subjected to reaction with metal salt (II): copper, cobalt, iron, zinc, manganese, magnesium in aqueous medium at neutral pH, exposing reaction mixture at room temperature, neutralisation of formed acid, water flushing, centrifugation and drying precipitate. According to invention to 1 l of 5.0·10-3 M aqueous solution of purified oligogalacturonic acid while stirring is added first 2 M aqueous solution of ammonia hydrate to pH 8, then 1 l of 4.5·10-2 M aqueous solution of metal acetate (II); formed gel-like precipitate is treated with 96 % ethanol at volume ratio of 1:2, held for 2 hours.

EFFECT: invention ensures delivery of biogenic metals (II), as well as removal of heavy metal cations (II) from body.

1 cl, 8 tbl, 4 dwg, 6 ex

 



 

Same patents:

FIELD: food industry.

SUBSTANCE: method for production of pectin and sugar containing food vegetal fibres of beet roots envisages initial raw materials washing at water temperature equal to 70-90°C and milling into fractions sized 5.0-20.0 mm. Simultaneously chips are sprayed with 30%-citric acid solution to provide for pH level in squeezed juice within the range of 4.0-4.5. Then one performs squeezing till pulp moisture content is equal to 70-80%, drying in a drying installation during the whole drying period at the product temperature within the range of 32-40°C, blown air humidity during the product drying being10-15%, till the ready product moisture content W=7-9%. The drying installation combines infrared and convective processes. Additionally, the obtained product is ground until homogeneous powdery condition.

EFFECT: method specificity is as follows: manufacture wastelessness, toxic reagents absence, efficiency and high performance due to raw materials maximum usage, environmental safety and minimum number of technological steps.

2 cl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to the pharmaceutical industry, namely to a method of obtaining pectins from a biomass of cultured tissues of plants Silene vulgaris (M.) G. The method of obtaining pectins with an increased content of galactose residues in side carbon chains from Calusa cultures includes raw material destruction, extraction with water, processing of the biomass with hydrochloric acid, water washing, extraction with ammonium oxalate solution, sedimentation of polysaccharide with ethanol, dialysis and lyophilising, with Caluse cultures being preliminarily grown for 21 hours on an agarised nutrient medium, which contains the ferment 1,4-β-D-galactozyltranspherase in a specified concentration, and as the raw material the biomass of cultured tissues of plants Silene vulgaris is applied.

EFFECT: method makes it possible to obtain physiologically active pectins, which have a specified structure and stable chemical composition.

1 tbl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biopolymers that can find application in chemical-pharmaceutical industry, medicine and veterinary science. The complex of pectin biopolymer and acetylsalicylic acid of formula wherein R=H, Me; n=98; m=2-11, prepared by a reaction of aqueous solutions of pectin and acetylsalicylic acid under mechanic-acoustic action with weight ratio of pectin: acid=1:(0.02-0.25) and ethanol precipitation of the prepared complex.

EFFECT: complex possesses lower toxicity, lower ulcerogenic action as compared to acetylsalicylic acid and no irritant action of skin and eye mucosa.

4 cl, 1 tbl, 7 ex

FIELD: food industry.

SUBSTANCE: invention relates to production of sugar beet fibres and may be used during production of a rheological properties regulator, a structure-forming gent and a thickener in food industry. The method envisages preparation of pulp from sugar beet pressure cake or chips with cellular tissue content no less than 18%, expressed pulp addition into a solution of an alkali reagent in softened water, water duty equal to 1:15-1:40, pH equal to 10-12, water temperature being 30-60°C. One performs fractional introduction of hydrogen peroxide with gradual temperature increase. The total quality of hydrogen peroxide taken is 25 kg of hydrogen peroxide with concentration equal to 30% per 50 kg of pressure cake/chips. When the temperature is 65-70°C one performs pH correction with 5-10% water alkali solution until pH is 9.0-10.0. Then one reduces temperature to 70-90°C within 20-45 minutes. Total bleaching time is 1-3 hours. Then one performs expression using a decanter centrifuge. Then pulp is washed, first - with softened water, then - with osmotic water. After each washing stage pulp is subjected to expression. Then one supplies finely dispersed ozone with further addition of 2.0-6.0% solution of Na2S2O3; one performs stirring and pulp expression using a decanter centrifuge. Then one performs repeated additional washing with further expression using a decanter centrifuge. As a result, a grainy wet product is produced.

EFFECT: production of a product wherein pectin substances are bound with a cellulose matrix which ensures good damping of fibres and a high degree of structure forming.

6 cl, 11 ex

FIELD: chemistry.

SUBSTANCE: invention relates to food industry and can be used in producing pectin from vegetable material. The method involves hydrolysis and extraction of vegetable material in an electromagnetic field, separating the solid and liquid phases, concentrating, depositing pectin and drying the pectin. Hydrolysis and extraction of the vegetable material is carried out with aqueous solution of citric acid and succinic acid at temperature of 80-90°C and pH=2 in an electromagnetic field with frequency of 25-29 Hz for 55-90 minutes. The citric acid and succinic acid are taken in ratio of 3:2, respectively. Further, the material is concentrated to pectin substance concentration of 5% and coagulated with 96% ethyl alcohol for 10 minutes. The coagulate is subjected to infrared drying in a vacuum at pressure of 0.08±0.02 MPa and temperature of 35-40°C to moisture content of the end product of not more than 7%.

EFFECT: invention increases output of pectin and increases complexing capacity thereof.

3 ex, 1 tbl

FIELD: food industry.

SUBSTANCE: method for processing silver fir wood green envisages raw material milling, the raw material treatment with an alkali solution, the raw material filtering to remove the resultant solution, acids separation by way of extraction with an organic solvent. After milling the raw material is treated with 0.1-0.5% water solution of a mineral acid at a temperature of 50±5°C with the filtered raw material subsequent two-times washing with water. Then the blended acid filtrates are concentrated by way of water boiling out in a rotor evaporator at a temperature of 60°C. Polysaccharides are sedimented from the resultant concentrate with excessive ethanol. The raw material remaining after polysaccharides extraction is subjected to treatment with an alkali and an organic solvent to separate triterpenic acids.

EFFECT: invention enables silver fir wood green processing to produce polysaccharides and triterpenic acids and to increase the target products yield.

2 ex

FIELD: food industry.

SUBSTANCE: invention relates to technical biochemistry, in particular - to determination of pectin substances quantity in vegetal raw material. "Standardised" solutions of pectin substances fractions are prepared. The fractions solutions are sampled. Saponification of pectin substances fractions in the samples of the solutions being analysed is performed with 2.5 ml of 40% NaOH solution. The pectin substances fractions are sedimented with 2.5 ml of concentrated HCl. The solutions being analysed are centrifuged in 50 ml test tubes, rotation frequency being no less than 500 rpm, during 5-7 minutes. The sediments of pectin substances fractions are suspended in distilled water in a titration cup on a magnetic stirrer for at least 30 minutes. One performs conductometric titration of the suspension of pectin substances fractions sediment, stirring. Following the conductometric titration results, graphs are drawn, relying whereon one identifies the volume of the titrant spent on pectin acid titration. Pectin substances fractions weight percentage is calculated from the formula: ω=176×0,2×V10×200×100, where 176 - pectin acid equivalent; 0.2 - titrant normality; V - titrant volume spent on pectin acid titration, ml; 200 - volume of the solution of the corresponding pectin substances fraction taken for analysis, ml; 10 - conversion to acid milliequivalents (1 ml of 0.1n NaOH corresponding to 0.1 acid milliequivalent); 100 - percentage conversion factor.

EFFECT: invention enables obtainment of accurate data with high coincidence of parallel analyses and ensures labour and time expenditures saving at all the process stages.

6 dwg, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to methods for preparing low-molecular pectin and may be used in pharmaceutical industry for preparing new therapeutic and preventive drugs, low-molecular sorbents. The method provides pectin hydrolysis in an aqueous solution of mineral acid and liquid phase separation from an insoluble residue of pectin. The low-molecular products of pectin hydrolysis are recovered from the liquid phase by settling them in an organic solvent with water. A hydrolysis feed material is low-etherified pectin of ratio max. 30%. The hydrolysis process is continuous in a continuous-flow machine. Temperature in a working chamber of the machine is maintained at 70-100°C. Feeding speed of mineral acid into the chamber is calculated by specific formula. The prepared liquid phase is neutralized to pH min. 4.0. Thereafter, the prepared liquid phase is used to settle the low-molecular products of pectin hydrolysis.

EFFECT: invention enables producing low-molecular pectin with no process loss of the feed material, substantially reducing oligogalacturonide destruction accompanying the hydrolysis process, and thereby improving the end product yield.

5 cl, 1 dwg, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to methods for preparing low-molecular pectin and may be used in pharmaceutical industry for preparing new therapeutic and preventive drugs, low-molecular sorbents. The method provides pectin hydrolysis in an aqueous solution of mineral acid with heating and liquid phase separation from an insoluble residue of pectin. The low-molecular products of pectin hydrolysis are recovered from the liquid phase by settling them in an organic solvent with water. A hydrolysis feed material is low-etherified pectin of ratio max. 30%. The hydrolysis process is continuous in a continuous-flow machine. Temperature in a working chamber of the machine is maintained at 70-100°C. Feeding speed of mineral acid into the chamber is calculated by specific formula. The prepared liquid phase is exposed to additional heat treatment in a flow heat exchange following the hydrolysis process. Temperature of the additional heat treatment process coincides with hydrolysis temperature. Heat exchange section capacity is determined by specific formula. The prepared liquid phase is neutralised to pH min. 4.0. Thereafter, the prepared liquid phase is used to settle the low-molecular products of pectin hydrolysis.

EFFECT: invention enables producing low-molecular pectin with no process loss of the feed material, substantially reducing oligogalacturonide destruction accompanying the hydrolysis process, and thereby improving the end product yield.

5 cl, 1 dwg, 4 ex

FIELD: food industry.

SUBSTANCE: invention relates to food industry. The method envisages dry beet pulp swelling in 30-40°C water at a ratio of 1:20 during 30-40 minutes. Then pulp completely swollen is squeezed and twice washed with cold water. Then the washed pulp is poured with a 0.5-2.0% enzyme preparation solution with high cellulolytic activity. The weight ratio of beet pulp and the enzyme preparation is chosen within the range of 1 :(13-15). The mixture is maintained at a temperature of 40-42°C during 1-2 hours while continuously stirred. Then the mixture is separated. One pours the produced pulp with milk whey at a ratio of 1:(13-15) and performs hydrolysis extraction during 2-2.5 hours at a temperature of 95-98°C while continuously stirring. Then one performs hydrolysate separation and filtration. Then the final product is packed.

EFFECT: invention allows to produce a food pectin beet extract with good organoleptic indices and ensure a non-waste technology of sugar beet processing.

3 ex

FIELD: biochemistry.

SUBSTANCE: invention refers to derivatives of 3'-ethynilcytidine represented by the formula (1); (where X is hydrogen atom, alkylcarbonyl group where alkyl fragment is non-branched or branched C1-C6alkyl group which can include as substitute(s) mono-or di-substituted with non-branched or branched C1-C6alkyl group aminogroup or alkoxycarbonyl group where alkoxy-fragment is a branched or non-branched C1-C6 alkoxygroup; One of Y and Z is a hydrogen atom or group (R1)(R2)(R3)Si-, and another is a group (R4)(R5)(R6)Si-; and each R1, R2, R3, R4, R5 and R6 that may be similar or different are non-branched or branched C1-C10 alkyl group or C6-C14aryl group) or to its salts. The invention also refers to the derivative of 3'-ethynilcytidine chosen out of compounds (1)-(17), to the pharmaceutical composition, to oncologic drug, to oral oncologic drug, to the use of derivative of 3'-ethynilcytidine, and to the method of tumor treatment.

EFFECT: new biologically active compounds with antitumor activity.

14 cl, 18 ex, 12 tbl

FIELD: medicine.

SUBSTANCE: invention refers to cobalamin derivatives of the formula , where Rb means spacer chelating group of the formula , where n is 2, 3 or 4; Rc, Rd, Re and RR denote hydrogen, and X is cyano; or where Rd indicates spacer chelating group of the formula , where n is 3; Rb, Rc, Re, and RR denote hydrogen, and X is cyano, or where Rb indicates spacer chelating group of the formula , where n is equal to 2; Rc, Rd, Re and RR denote hydrogen, and X is cyano. These compounds are accumulated to a much lesser extent in blood and non-malignant organs such as kidneys and liver, compared with the rate of accumulation in neoplastic tissues, and moreover they are quickly removed from blood. The invention also refers to injectable pharmaceutical composition for diagnosis of tumors including cobalamin derivative carrying a radioactive metal atom. In addition, the invention refers to diagnosis of tumors in mammals, including (a) compliance vitamin B12-free diet for mammals predisposed to neoplastic disease, (b) subsequent administration of cobalamin derivative carrying a radioactive metal atom. The invention also refers to use of cobalamin derivative carrying a radioactive metal atom, in method of diagnosis of tumors in mammals and to obtain pharmaceutical composition designed for use in method of diagnosis of neoplastic diseases in mammals.

EFFECT: improvement of composition application.

13 cl, 2 tbl, 6 dwg, 35 ex

FIELD: medicine.

SUBSTANCE: method involves a combined administration of the preparations Enterosgel, Kudesan and Pantogam. Enterosgel is administered orally in the form of gel 30 minutes before meals in a dose of 1 teaspoon two times a day for 7 days. Kudesan is also administered orally during meals before noon in an age dose of: 10-16 drops (15-24 mg) in the children aged 5 through 7 years old, 16-20 drops (24-30 mg) in the children aged 8 through 10 years old, pre-dissolved in a small amount of boiled water or other beverage of room temperature, once a day in the course of 30 days. Pantogam is administered orally 15-20 minutes after meals in an age dose of: 500 mg 2 times a day in the children aged 5 through 7 years old, 500 mg 3 times a day in the children aged 8 through 10 years old in the course of 30 days.

EFFECT: complex administration regiment provides the effective correction of cognitive disorders in the given group of children by a synergetic detoxification, antioxidant and neuroprotective action of its components.

3 cl, 4 tbl

FIELD: chemistry.

SUBSTANCE: application of 5% water solution of sodium and potassium salts of humic acids, obtained from leonardite brown coal, in dose 10.0 mg/kg as membrane-protective preparation.

EFFECT: application of composition described above makes it possible to extend arsenal of membrane-protective preparations from low-toxic, cheap, available natural raw material.

2 dwg, 2 ex

FIELD: pharmacology.

SUBSTANCE: agent possessing detoxification activity, containing succinic acid, 10% ethanol extract of propolis, polyethylene glycol 400, polyethylene glycol 1500, polyethylene glycol 4000, Cremophor CO-40, Tween-80 and purified water in certain proportions.

EFFECT: agent possesses the detoxification action, the uniform release of the active substances and the pronounced prolonged action.

2 cl, 4 dwg, 4 tbl, 8 ex

FIELD: medicine.

SUBSTANCE: invention relates to radiation and experimental biology, biotechnology and medicine, namely to means of radiological protection and to stimulators of colony formation of stem cells. Invention can be used in research and clinical practice for protection of living organisms in case of exposure to ionising radiation, in emergency and military medicine, in case of emergency situations as means for protection against radiation injury. Claimed is application of preparation gamma-plant as radioprotector with colony-stimulating properties for increasing survival and improving state of organisms, subjected to irradiation. Gamma-plant is non-toxic polysaccharide of vegetable origin, registered by Ministry of Health of the Russian Federation and allowed for medical application; Gamma-plant is included into Russian Pharmacopoeia in section FG-9 "non-narcotic analgesics".

EFFECT: invention makes it possible to increase efficiency of colony formation of stem cells in spleen 2,0-2,3 times, increases survival of experimental animals to 95% with coefficient of dose reduction factor DRF, equal 2-2,2.

2 tbl

FIELD: medicine.

SUBSTANCE: invention refers to veterinary science, namely to clinical pharmacology and veterinary therapy. The method consists in administering the complex iron-dextran preparation Ferranimal-75M intramuscularly on the 5th day of calf's life in a dose of 3 ml in a combination with an intramuscular injection of the preparation Hydropeptone in a dose of 10 ml. Ferranimal-75M is injected 10 days later in a dose of 2 ml in a combination with an injection of Hyropeptone 5 ml intramuscularly in different points.

EFFECT: method provides higher antioxidative activity of calf's blood serum, reduced pro-oxidant action of iron and incorporated radionuclides, as well as higher iron accessibility in treating and preventing iron-deficiency anaemia in calves exposed to the chronic incorporated radiation.

FIELD: medicine.

SUBSTANCE: first, Fraxiparine is inhaled for 5-10 minutes. A dialysis solution for peritoneal dialysis containing extranyl is administered into the abdominal cavity 1-1.5 hours later through a catheter. The solution additionally contains: albumin in the concentration of 35-40 g/l, heparin in the concentration of 500-750 Unit/l and papaverine in the concentration of 50-100 mg/l. Every 6-8 hours after the beginning of exposure of the dialysis solution in the abdominal cavity, the Fraxiparine inhalations are repeated. The time of a single exposure of the dialysis solution in the abdominal cavity makes 12-18 hours.

EFFECT: method provides effective body detoxification in the given category of patients by providing a high toxin clearance owing to a continuous high osmotic concentration of the solution and the dilatation of pre-capillary peritoneal arterioles, prevented inspissation of the albumin solution to the colloidal state and its inactivation that reduces a rate of the dialysis solution exchanges.

3 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, namely to an immunomodulatory composition for injection into a mammal. The immunomodulatory composition for injection into a mammal containing a hydrolysate prepared by acid and/or enzymatic hydrolysis of one or more bioresources specified in a group consisting of bivalve molluscs, annelids, leeches, and water taken in certain proportions. A method for preparing the immunomodulatory composition for injection into a mammal. A method of treating a pathological condition in a mammal in need thereof involving the injections of the immunomodulatory composition into the above mammal. Using the composition for normalising metabolism into the mammal in need thereof.

EFFECT: composition enables extending the range of products with immunomodulatory activity for injections.

19 cl, 7 tbl, 7 ex

FIELD: veterinary medicine.

SUBSTANCE: method of treatment of calves with Simuliidotoxicosis is that 40% solution of urotropine is administered intravenously to calves at a dose of 1.5 ml per 10 kg of animal body weight.

EFFECT: improvement of efficiency of the method.

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