Formulations of fluorescent gel and use thereof
SUBSTANCE: invention relates to monitoring of microbial contamination and can be used in health and public catering. Described is composition to determine whether surface cleared of microbial contamination. Composition contains from 0.001 to 10 wt% of cationic or anionic optical bleaching agent and from 0.001 to 5.0 wt% of complexing agent with opposite charge. Said complexing agent selected from a group consisting of surfactant, modified cellulose, modified guar modified acrylic compounds, modified urethane, polyvinylpyrrolidone and ethoxycarboxyilates.
EFFECT: formulations of fluorescent gel are stable, fluoresce under effect of UV radiation and leave no traces after drying and removal.
20 cl, 12 tbl, 4 ex
SUBSTANCE: rock samples are crushed, the crushed rock is treated with benzo-alcoholic solvent (4:1), kept for 18-20 hours at a temperature of 60°C, settled and filtered. The resulting extract is irradiated with UV light with the resonance mercury lines of 184.9 and 253.6 nm, the luminescence is recorded with the photometer with a set of absorption light filters, the light and heavy fractions of hydrocarbon compounds are determined, the interference of luminescence rocks is eliminated by the shear of the measured luminescence band into the shorter wavelength area.
EFFECT: invention enables to improve the accuracy of determining saturation with oil substances of rocks by improving the accuracy of analysis in extraction on the calibration graphs.
SUBSTANCE: method includes using a demixing extraction system - water-antipyrine-acid, wherein antipyrine and naphthalene 2-sulphonic acid are taken in molar ratio of 1:1, heated to melting point of 97°C, 10.0 ml of the analysed aqueous solution is added to 1 ml of the obtained melt of the organic salt antipyrinum naphthalene-2-sulphonate, intensely shaken and held until demixing into an upper aqueous phase and a lower organic phase and the lower organic phase is analysed for fluorescence intensity.
EFFECT: high sensitivity and reliability of analysis.
1 ex, 3 tbl, 5 dwg
SUBSTANCE: sensor layer based on transparent silicate xerogel, obtained using a sol-gel synthesis method in the presence of Nile red organic dye, is illuminated with light with wavelength of 560-610 nm and fluorescence intensity of the sensor layer is detected in the 630-680 nm wavelength range. Presence of gas-phase acetone is determined from the change in fluorescence intensity.
EFFECT: invention enables to determine presence of acetone vapour in air using small-size devices within 5 minutes.
SUBSTANCE: invention relates to a novel method of obtaining fluorescent catecholamines selected from dopamine and adrenalin, and their metabolites, selected from homovanillic and vanillin-mandelic acids, by a method of derivation. The compounds can be used as highly sensitive and selective markers for the determination of various diseases. The method of derivation includes oxidation of the initial compounds and their interaction with amines that form condensed structures in a medium of the CAPS-buffer solution or glycin - KOH 0.2 mM hydrogen peroxide in the presence of horseradish peroxidase as a catalyst. The process in preferably carried out in a 0.1 M buffer solution with the concentration of horseradish peroxidase 0.01-1 mcM; concentration of hydrogen peroxide - 100 mcM, amine concentration - 0.1-33 mM; concentration of catecholamines and metabolites - 0.03-1 mcM.
EFFECT: method is simple and producible as it does not require higher temperature and is realised in a water solution.
2 cl, 2 dwg, 3 ex
SUBSTANCE: membrane-bound red cell haemoglobin by giant combined diffusion (GCD) spectroscopy is analysed by the use of nanostructured coatings in the form of ring silver nanostructures having the hierarchical structure. Silver ring rims consists of connected micron-scale porous silver aggregates, the surface of which comprises rounded silver nanoparticles 2-100nm embedded into a matrix. The red cell immobilisation time on the nanostructured coatings makes 5-40 minutes, and GCD spectra are generated by green laser at wave length 514 or 532 nm.
EFFECT: invention enables diagnosing membrane-bound haemoglobin in undamaged red cells by the giant combined diffusion.
4 cl, 4 ex, 1 dwg
FIELD: measurement equipment.
SUBSTANCE: group of inventions relates to measurement and control of presence of hydrophobic pollutants. The invention presents a version of a monitoring method of presence of one or more types of hydrophobic pollutants during paper manufacture, which involves the following: a. obtaining a fluid medium sample from the specified paper manufacturing process; b. measurement of turbidity of the specified fluid medium sample; c. selection of a hydrophobic colouring agent capable of interaction with the specified pollutants in the specified fluid medium and fluorescence in the specified fluid medium; d. addition of the specified colouring agent to the specified fluid medium and exposure during the time required for interaction of the specified colouring agent with the specified pollutants in the specified fluid medium; e. measurement of colouring agent fluorescence in the specified fluid medium; and f. setting of correlation between fluorescence of the colouring agent and concentration of the specified pollutants; with that, if turbidity measured at stage (b) is more than 2000 nephelometric turbidity units (NTU), then the specified sample is diluted or separated additionally prior to addition of the specified colouring agent at stage (d) and measurement of fluorescence.
EFFECT: invention presents a measurement method of efficiency of one or more chemical reagents reducing the number of one or more hydrophobic pollutants during paper manufacture Quick, accurate and reliable monitoring is achieved.
20 cl, 3 dwg, 3 ex
FIELD: physics, control.
SUBSTANCE: invention is intended for monitoring a plurality of discrete fluorescence signals, particularly for DNA sequencing using fluorescently labelled nucleotides. The detector (118) comprises a plurality of pixels (130) for individually detecting said fluorescence signals from the plurality of fluorescent signal sources (104), wherein each pixel (130) comprises a predetermined number of at least two detection elements (D1, Dn) for detecting a received fluorescent signal and for generating detection signals, as well as a signal conversion circuit (140) for receiving said detection signals from said at least two detection elements (D1, Dn) and for generating a pixel output signal indicating which of said at least two detection elements (D1, Dn) generated the strongest detection signal.
EFFECT: monitoring a plurality of discrete fluorescence signals.
15 cl, 7 dwg
SUBSTANCE: invention relates to chemistry of organometallic compounds, particularly to alkynyl phosphine gold-copper complexes which dissociate in a solution to form ions. The gold-copper alkynyl phosphine complexes are capable of forming covalent conjugates with proteins, thereby turning into a water-soluble form, exhibit luminescent properties and can be used as labels for fluorescence microscopy and in luminescent analysis.
EFFECT: improved properties of the compounds.
5 dwg, 4 tbl, 3 ex
SUBSTANCE: apparatus comprises a fluorescent-reflective spectrometer, which includes an illumination system and spectrometric system connected to a Y-shaped fibre-optic probe. The apparatus is further provided with two channels, one of which intended for supplying liquid to the investigated organ to wash off blood and is connected to a pump, and the other channel is intended for sucking the liquid and blood from the investigated organ and is connected to a pump. Both channels and the distal end of the fibre-optic probe are placed in a ferrule to form a fibre-optic probe. The ferrule is in the form of a metal cylinder with a socket at the end which is adjacent to the investigated organ.
EFFECT: high accuracy and consistency of measurement results, enabling investigation of the heart within the body.
7 cl, 5 dwg
SUBSTANCE: invention relates to biotechnology and represents method of spectral analysis of fluorescent properties of DNA nucleotide successions. Claimed invention can be applied for genetic diagnostics, research of mitogenetic irradiation of cells, study of coding hereditary and proliferative information. Method includes preparation of water solutions of dyes of basic fluorescence colours. Background fluorescence of dye is measured in respective detection channels by means of fluorescent detector FDG-001. DNA sample is added to dye solution in quantity 150-200 ng. Fluorescence of dye solutions is measured. Results of analysis are registered in form of presentation of values in conventional units of positive or negative increase of fluorescence of dyes relative to their initial background before addition of DNA sample in form of building columnar diagrams or curves of signal growth dynamics in time. Results of dye fluorescence growth are interpreted.
EFFECT: claimed invention makes it possible to determine nucleotide rebuildings of telomeric DNA ends, point mutations, polymorphisms of gens, chromosome rebuildings, and change of karyotype or cell genome.
15 cl, 16 dwg, 1 tbl, 6 ex
SUBSTANCE: invention describes a skin and hand cleaner containing the following components: a) 5-70 wt % alkyl ester and/or diester, b) 0-40 wt % surfactant, selected from a group comprising ethoxylates of fatty alcohols, sulphoesters of fatty alcohols and salts of sulphated and/or sulphonated fatty acids, c) 0.5-10 wt % thixotropy agent, such as organic bentonite, and more than 0.1 wt % hydrophilic pyrogenic silicic acid, d) 0-30 wt % of one or more abrasive agents, e) 0-5 wt % physiologically compatible carbonic ester, f) 0 to less than 10 wt % water, g) optionally one or more viscosity stabilising agents, h) optionally other cosmetic auxiliary substances.
EFFECT: cleaner removes stubborn dirt from skin and hands, has a fluidity point which enables to prevent sedimentation if there is need to use abrasive particles, is easy to make, is homogeneous and stable and is easy to store.
16 cl, 29 ex, 3 tbl
SUBSTANCE: invention relates to cosmetology, detergent compositions and discloses a universal dry agent in form of a powder containing ground parts of saponin-containing plants which release surfactants immediately upon activation, and specifically parts of a soap root or a soap tree in amount of 5-99.9 wt %. Natural auxiliary components which perform stabilising and anti-caking functions in the detergent are zeolite, ground mustard, salt, guar or quartz in amount of 0.1-95 wt %. The powder has particle size of 0.005-2000 mcm.
EFFECT: detergent is save for use, has a long shelf life, does not cake and is characterised by simple use of saponin-containing plants as a detergent base.
1 tbl, 1 ex
SUBSTANCE: aqueous cationic surfactant system contains microfibre cellulose in concentration of 0.05-0.09 wt %, a cationic surfactant in concentration of 0.05-50 wt % of the active surfactant and suspended inclusion particles. The inclusion particles are preferably suspended air bubbles or suspended granules. The method involves mixing the microfibre cellulose with water and stirring, adding the cationic surfactant and stirring, adding inclusion particles and stirring, the formed system being transparent and the inclusion particles being suspended in that system.
EFFECT: improved suspension properties and viscosity of the composition.
15 cl, 1 tbl, 5 ex
SUBSTANCE: detergent contains the following in wt %: sodium alpha-olefin sulphonate 13-16; alkyldimethylamine oxide or cocoamidopropylamine oxide 2-4; sodium sulfoethoxylate 0.1-4; tetrasodium salt of ethylenediamine tetraacetic acid 0.1-0.4; sodium chloride 0.1-6; skin protection additive 0.1; dyes 0.001-0.01; fragrance 0.3-0.5; 2-methyl-4-isothiazolin-3-one 0.05-0.1; orthophosphoric acid 0.1-0.5; demineralised water - the rest up to 100. The skin protection additive is a vitamin E extract or an aloe vera gel or a peach extract or a lemon extract or an apple extract or an almond milk extract.
EFFECT: improved detergent properties, reduced consumption and high environmental friendliness of the detergent.
1 tbl, 2 ex
SUBSTANCE: invention relates to compositions for purification of solid surfaces, containing acidic component; one or more surfactants; surface-modifying polymer; component for control of unpleasant odour, which contains effective quantity of two or more volatile aldehydes, selected from 2-ethoxybenzylaldehyde, 2-isopropyl-5-methyl-2-hexenal, 5-methylfurfural, 5-methyl-thiophene-carboxaldehyde, adoxal, p-anisic aldehyde, benzylaldehyde, bourgeonal, cinnamic aldehyde, cymal, decyl aldehyde, floral super, florhydral, helional, lauryl aldehyde, ligustral, lyral, melonal, o-anisic aldehyde, linoacetaldehyde, P.T.Bucinal, thiophenecarboxaldehide, trans-4-decenal, trans trans 2,4-nonadienal, undecyl aldehyde; and water carrier ,with component for unpleasant odour control containing from 35% to 60% of volatile aldehydes with B.P. 250°C or higher and ClogP 3.0 or higher; or 25% of aldehydes which have B.P. approximately 250°C or lower and ClogP approximately 3 or lower; or 10% of aldehydes, which have B/P/ 250°C or lower and ClogP 3.0 or higher; or from 10% to 30% of aldehydes, which have B.P. 250°C or higher and ClogP 3.0 or lower.
EFFECT: invention provides neutralisation and concealing wide spectrum of unpleasant odours, including amine and sulphur unpleasant odours.
17 cl, 1 dwg, 10 tbl, 23 ex