Recombinant dna pa4, recombinant dna pqe 30-pa4, providing obtaining polypeptide a4, esherichia coli strain m 15-a4, transformed with recombinant plasmid dna pqe 30-pa4, and expressing recombinant polypeptide a4, recombinant polypeptide a4, possessing ability to selectively bind hsa, affine sorbents (versions) and methods for hsa and igg removal from blood serum (versions)
SUBSTANCE: inventions relate to field of molecular biology and deal with recombinant DNA pA4, recombinant plasmid DNA pQE 30-A4, Esherichia coli strain M15-A4, recombinant polypeptide A4, possessing ability to selectively bind human serum albumin (HSA), affine sorbent, containing such polypeptide, affine combined sorbent and methods for successive removal of HSA and IgG from blood serum. Characterised affine combines sorbent is obtained by mixing affine sorbent, containing said recombinant polypeptide A4, and analogous affine sorbent, in which known IgG-binding polypeptide is used as ligand.
EFFECT: claimed inventions can be used in medical practice to deplete blood serum of two proteins, albumin (HSA) and immunoglobulin G (IgG), present in it in high concentrations Removal of two major proteins from blood serum will make it possible to identify other proteins, present in serum in lower concentrations.
8 cl, 9 dwg, 7 ex, 1 tbl
SUBSTANCE: invention relates to a method of screening a non-teratogenic substance comprising bringing a test substance into contact with cereblon or a fragment of cereblon, evaluating the capacity of the test substance to bind to cereblon or the fragment of cereblon, and selecting a test substance that does not bind to cereblon or the fragment of cereblon or a test substance exhibiting lower capacity to bind to cereblon or the fragment of cereblon compared with thalidomide.
EFFECT: improved method.
8 cl, 19 dwg, 8 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: there are offered: use of the tri-substituted glycerol compounds of formula (I) in preparing a therapeutic agent with said therapeutic agent applied with at least one other therapeutic agent containing one or more additional active ingredients specified in a group consisting of antimetabolites, herbal alkaloids, topoisomerase II inhibitors, proteasome inhibitors; related combination and an in vitro method for determining sensitivity of hematological malignant tumours to a combination of the therapeutic agents as specified in the present invention.
EFFECT: what is presented is a synergic cytotoxic effect of the compound of formula (I) of edelfosine in a combination with the proteasome inhibitor Bortezomib, with the antimetabolite fludarabine, cytarabine, the topoisomerase II inhibitor edelfosine.
11 cl, 10 dwg, 4 tbl
SUBSTANCE: invention includes compositions, methods and cell lines related to insect control. Substance of invention includes compositions that contains vegetable essences purposely acting at least at one receptor of insects selected from the following - tyramine receptor , olfactory receptor Or83b or olfactory receptor Or43a, as a result of which intracellular levels cAMP, Ca+2 or both vary in insects. Also invention includes method of insect control, line of insects cells including sequence of nucleic acid of above mentioned receptors and methods for selection of composition by activity in respect to insects with application of specified line of insect cells.
EFFECT: development of softer sparing compositions that are not toxic for mammals and other types.
20 cl, 34 dwg, 1 tbl, 33 ex
FIELD: genetic engineering.
SUBSTANCE: invention refers to polypeptides GPCR Drosophila melanogaster (DmGPCR) and polynucleotides detecting and coding such polypeptides. Construction of expressing vectors based on specified polynucleotides and production of containing host cells enables to apply DmGPCR as insecticides. Besides, invention refers to methods of DmGPCR-bindnig, method of DmGPCR expression and activity modulator identification, method of insect population control using DmGPCR-antibody, DmGPCR antisense polynucleotides, DmGPCR binding partner or modulator.
EFFECT: new application of polypeptides.
35 cl, 7 tbl, 10 ex
FIELD: molecular biology.
SUBSTANCE: invention provides a method to determine hydrophobic ligands of lipid-carrying proteins and to find out lipid-binding and lipid-carrying activities of proteins, which would clarify lipid-protein interactions in vivo. Method comprises preparation of donor and acceptor phases, which are then juxtaposed them, while separating them by porous barrier, after solution of test protein in SET buffer is passed and then acceptor part is extracted by tertiary mixture of equal parts of chloroform, methanol, and diethyl ether to recover protein solution.
EFFECT: simplified procedure.
3 dwg, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a high-sensitivity method for measuring the amount of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts. The high-sensitivity method for measuring the amount of glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts is characterised by the fact that a mixture of individual's blood plasma with methanol or ammonia water in the specific concentration is introduced into a solid phase having the reverse-phase distribution function and the anion exchange function; the solid phase is then washed with a cleaning fluid that is a single-component fluid or a mixed fluid of at least two components specified in a group containing water, alkali, alcohol and acetonitrile. That is followed by elution from the solid phase in acid alcohol specified in formic acid - methanol or formic acid - ethanol; that is followed by the stage of measuring glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts by liquid chromatography - mass spectrometry or liquid chromatography - mass spectrometry/mass spectrometry.
EFFECT: high-sensitivity method enables detecting and measuring the amounts of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts.
4 dwg, 17 tbl, 7 ex
SUBSTANCE: invention relates to forecast of ageing processes of the synthetic polymer materials (SPM) depending on duration of their operation or storage. Analysis of the volatile organic compounds (VOC) migrating from SPM is performed by active sampling for sorbent, with further thermal desorption and gas chromatographic analysis. Forecast of the ageing processes of the material and estimation of toxicity of the gas discharge are performed as per dynamic of the qualitative and quantitative composition of gas discharge components in SPM initial state, and during artificial climatic thermal-humidity ageing. Dynamics analysis of the total gas discharge (ΣT) from each material is performed for all substances migrating from studied SPMs. Change of toxicity is estimated and forecast of the material ageing is performed as per developed indices of total gas discharge (ΣT) and as per hygienic index P=(ΣTinitial/ΣTn)/V, where Tinitial and Tn are indices of toxicity of gas discharge of each substance in initial state and after ageing, respectively, and ΣTinitial and ΣTn are total indices of toxicity of gas discharge of all components of SPM in initial state and after ageing, V is duration of ageing (year, month).
EFFECT: invention ensures high accuracy of the method of VOC qualitative and quantitative composition determination in gas discharge during materials ageing and the analysis results repeatability.
SUBSTANCE: sample under study is placed in a sealed container of inert material, thermostated to the temperature above 25°C but below the destruction temperature of the biological object under study. From the thermostated sample the sampling of gas-vapour phase is carried out, which is examined by chromatography-mass spectrometry method by separation on the chromatographic column. Then the components of mixture of the gas-vapour phase are recorded as a series of chromatographic peaks in the chromatogram and are identified on time of their exit on the chromatogram and mass spectrum. Calculation of the concentration of alcohols is carried out in accordance with the obtained chromatogram according to the relevant peak areas of components of mixture of the gas-vapour phase.
EFFECT: increased sensitivity, accuracy and reliability of the identification and quantitative research, preservation of the object under study for possible repeat or additional studies.
1 tbl, 8 dwg
SUBSTANCE: method of simultaneous determination of methanol and diethylene glycol in natural and sewage water by LC method with separation of said components in chromatographic column in flow of eluent, which represents sulphuric acid solution in deionised water with molar concentration 2.5 mmole/dm3; registering difference of indices of refraction of eluent solution and solutions, which contain target components and processing results of measurements by method of absolute calibration. Sample preparation does not require dilution of analysed water sample up to content of target components 10 g/dm3, more concentrated samples are diluted with deionised water.
EFFECT: quickness and simultaneousness of determination of diethylene glycol and methanol content.
SUBSTANCE: described is an alcohol thermal hydration chamber and an apparatus for real-time determination of isotopic composition of non-exchangeable hydrogen and deuterium atoms in ethanol samples, comprising: A) an alcohol thermal dehydration chamber, B) an indicating device which has a pyrolysis reactor (26) and a continuous flow isotope spectrometer and is connected to the alcohol thermal dehydration chamber by C) a system of valves, connectors and capillary tubes which are used to transfer the analysed sample and clean the alcohol thermal dehydration chamber; the procedure for real-time determination of isotopic composition of non-exchangeable hydrogen and deuterium atoms in ethanol samples and the procedure for off-line preparation of ethene (ethylene) gas by means of the alcohol thermal dehydration chamber and for the identity and geographical origin of wines, alcoholic beverages, fruit juices, honey and etc.
EFFECT: high accuracy of determination.
9 cl, 3 dwg
SUBSTANCE: invention can be used for simultaneous determination of content of ions of transition metals Fe(III), Fe(II), Cu, Pb, Zn, Ni, Co, Cd, Mn in natural, surface, waste, underground water and water extracts of saline soil. High-performance liquid chromatography is used for simultaneous determination of content of ions with separation of the ions in a chromatographic column in eluent flow. The eluent consists of a solution of sodium octane sulphonate, sodium hydrotartrate and acetonitrile in dionised water. Mixing is then carried out in a post-column reaction module with a reagent which is a solution of PAR ([4-(2-pyridylazo) resorcinol], glacial acetic acid and aqueous ammonia in deionised water. Further, the difference in optical absorption of the eluent and complexes of the determined ions with the added reagent is detected using spectrophotometric detector in the visible spectrum at 520 nm.
EFFECT: increased accuracy, faster simultaneous determination of content of transition metal ions in natural, surface, waste, underground water and water extracts of saline soil.
FIELD: chemical technology.
SUBSTANCE: invention relates to a method for synthesis of ester perfluorinated derivative by using a chemical reaction. This reaction represents the fluorination reaction of the parent compound as a raw, the reaction of chemical conversion of fragment of ester perfluorinated derivative to yield another ester perfluorinated derivative or the interaction reaction of carboxylic acid with alcohol under condition that at least one or reagent, i. e. carboxylic acid or alcohol, represents a perfluorinated compound wherein indicated perfluorinated derivative of ester represents a compound comprising a fragment of the formula (1):
with a boiling point 400°C, not above. The reaction time for carrying out abovementioned chemical reaction is sufficient to provide the required yield of ester perfluorinated derivative and wherein this yield of ester perfluorinated compound is determined by the gas chromatography method by using a nonpolar column. Also, invention relates to a method for pyrolysis of ester perfluorinated derivative with a boiling point 400°C, not above, to yield the dissociation product wherein this product represents a derivative of acyl fluoride or ketone and wherein pyrolysis time is sufficient to provide the required degree of conversion of ester perfluorinated derivative and wherein the indicated conversion degree of ester perfluorinated derivative is determined by gas chromatography method by using a nonpolar column. Also, invention relates to a method for analysis of ester perfluorinated derivative with a boiling point 400°C, not above, that involves analysis of ester perfluorinated derivative in a sample containing ester perfluorinated derivative by gas chromatography method by using a nonpolar column wherein ester perfluorinated derivative represents compound comprising a fragment of above given formula (1).
EFFECT: improved method of synthesis.
8 cl, 1 dwg, 2 ex
SUBSTANCE: invention relates to the field of biotechnology and can be applied for the selection of affine molecules. Constructed is a plasmid DNA pGST/APT/X 6193 bp long, with the weight of 4.09 MDa, containing as a genetic marker the gene β-lactase and unique sites of the recognition of restriction endonucleases, located at the following distance to the right from the site NdeI: BgIII 665bp, BamHI 779 bp., XhoI 801 bp. The plasmid pGST/APT/X consists of a NdeI-XhoI DNA fragment of the commercial plasmid pET22b(+) and nucleotide sequence SEQ ID NO:1, inserted by sites NdeI-XhoI and combining in its composition the sequences, which code a fragment of a gene of glutathione-S-transferase, a peptide substrate of a lethal factor of anthrax RRKKVYPYPME, peptide GGLNDIFEAQKIEWHED, biotinylated in vivo under the influence of E.coli biotin-ligase, and a linker sequence, substituted by genetic-engineering manipulations by sites of restriction endonucleases BamHI and XhoI for a sequence, coding a target protein B-subunit of shiga-toxin I.
EFFECT: invention makes it possible to carry out the selection of highly specific aptamers to the said target protein.
2 cl, 4 dwg, 4 ex
SUBSTANCE: invention relates to biotechnology, the modified polypeptide having homoserine-O-acetyltransferase activity having the amino acid sequence of SEQ ID NO:17 or at least 95% homologous thereto, in which the amino acid at position 111 from the start amino acid methionine, of the sequence is substituted with glutamic acid. The invention also relates to a method for producing O-acetyl homoserine, comprising culturing the microorganism belonging to the genus Escherichia, transformed with polynucleotide, encoding the said polypeptide.
EFFECT: high production yield of O-acetyl homoserine.
18 cl, 5 dwg, 3 tbl, 5 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: inventions concerns recombinant DNA pA3, recombinant plasmid DNA pQE 30-pA3, strain E.coli M 15-A3, recombinant polypeptide A3, test systems for the qualitative detection and quantitative determination of microalbuminuria. The characterised recombinant DNA pA3 is produced by a polymerase chain reaction with the use of chromosomal DNA of the strain DG 13 of group B streptococci and structured primers. An amplification fragment has been cloned by a system of expression vectors pQE-pQE 30 in E.coli M 15 to produce recombinant DNA pQE 30-pA3, which codes an amino acid sequence of the recombinant polypeptide A3 having an ability to bind human serum albumin (HSA) selectively.
EFFECT: presented inventions can be used in the medical practice and industry for producing the test systems for the detection and quantitative determination of microalbuminuria - one of the earliest signs of renal irritation in the patients suffering from diabetes mellitus and essential arterial hypertension.
6 cl, 9 dwg, 1 tbl, 8 ex