Method of predicting risk of reduction of child's health level at age of 12-16
SUBSTANCE: invention relates to medicine, namely to pediatrics, and can be applied for predicting reduction of child's health level at the age of 12-16. For this purpose an hour after meal and 15 minutes before mouth fluid sampling oral cavity is washed with 0.9% sodium chloride solution. Analysis of child's mouth fluid with determination of ratio of immunoglobulin A concentration to immunoglobulin G concentration is performed. Analysis is carried out twice with difference in 14 days. In case of 1.4 fold and higher reduction of immunoglobulin A concentration to immunoglobulin G concentration ratio during second analysis child is allocated to group of risk of health level reduction.
EFFECT: application of claimed method makes it possible to carry out screening in the process of carrying out prophylactic medical examination to plan health-improving procedures in groups of dispensary observation.
The technical field to which the invention relates
The invention relates to medicine, namely to Pediatrics.
The level of technology
The formation of children's health is one of the main tasks of domestic medicine. [Kuchma V. R. guidelines on hygiene and health of schoolchildren. Moscow: Russian public health Association, 2000. - P. 18]. Sharply raises the issue of developing a publicly accessible, inexpensive, informative methods of predicting the risk of reducing the level of health of the child. Such screening is required during clinical examination, and for planning recreational activities in groups of dispensary observation.
There are various ways of predicting the risk of reducing the level of health of the child. The most informative are invasive immunological methods, for most of them it is necessary to perform venous blood sampling, which requires special equipment, trained personnel and the consent of the child's parents [Method of predicting the risk of reducing the level of resistance of the organism to acute respiratory diseases in children aged 3-7 years on immunological indicators / Fedotova, T. A., Zhukov S. V., Kushnir, S. M., Antonova L. K., Kalinina O. V. // RF Patent for the invention №2445630 from 20.03.2012]. At the same time, there are methods and non-invasive methods.
The known� “a Method of predicting the decline and adverse health outcomes in children of early age” [a Method of predicting the decline and adverse health outcomes in children of early age/ Antonova L. K., Kushnir S. M., Malinin, A. N., Shmatov G. P., Kulakova N. And. // RF patent for the invention №2411002 from 10.02.2011]. The authors propose to judge about the risk of reducing the level of child health indicators of autonomic regulation. The methodology proposed for newborns, but can be applied to older children. However, the technique requires special equipment (electrocardiograph, or vegetarische) and the trained professional, which complicates its use in mass screening. It should be noted that in adolescence often occurs syndrome vegetative dystonia, in addition, the autonomic nervous system older child exposed to emotional stress and the nature of microsocial relations before the examination. Thus, the use of indicators of the autonomic nervous system to predict the risk of reducing the level of health of the child aged 12-16 years are not appropriate.
One of the most promising variants to predict risk of reducing the level of health of the child at any age is the assessment of immunological parameters in the saliva of the child. In this case, it is possible to identify a violation of one of the components of the immune response. The methodology is applied to predict the risk of reducing the level of resistance of the organism to acute respiratorysystem in children [Method of predicting the risk of reducing the level of resistance of the organism to acute respiratory diseases in children aged 3-7 years, according to the index of the avidity of immunoglobulin A / Fedotova T. A., Zhukov S. V., Kushnir, S. M., Antonova L. K., Kalinina O. V. // RF Patent for the invention №2445915 on 27.03.2012].
As a prototype we have chosen “Method of predicting the risk of reducing the level of resistance of the organism to acute respiratory diseases in children aged 3-7 years, according to the index of the avidity of immunoglobulin A” [a Method of predicting the risk of reducing the level of resistance of the organism to acute respiratory diseases in children aged 3-7 years, according to the index of the avidity of immunoglobulin A / Fedotova, T. A., Zhukov S. V., Kushnir, S. M., Antonova L. K., Kalinina O. V. // RF Patent for the invention №2445915 on 27.03.2012].
The inventive method consists in the fact that the child determine the concentration of secretory immunoglobulin A in saliva in parallel in two samples, one of which is added urea (0,3 mol/l), determine the avidity index by dividing the value of the concentration of secretory immunoglobulin A in the sample with urea at concentrations of secretory immunoglobulin A in the sample without urea, and when the value of the avidity index of less 0,089 or more of 0.27 of a child is considered at risk of reducing the level of resistance of the organism to acute respiratory diseases
The disadvantage of this method is that:
- examines only one element of the immune response - protect the mucous membranes of dyatel�different ways from infections,
- for research requires trained specialist (preparation of two solutions with the addition of the precise concentration of urea),
use in mass surveys is difficult due to the need to maintain two parallel samples, the probability of error due to the inclusion of one child samples from different children,
- difficulties in applying standard tablets for ELISA studies (requires changes (the urea) in half of the cells),
- does not take into account the possibility of changes in the concentration of immunoglobulin a in saliva associated with food intake or prolonged lack of food intake,
- ignores the dynamics of the intensity of the immune response.
In the inventive method listed deficiencies have been corrected.
Disclosure of the invention
The object of the invention is to assess the risk of reducing the level of health of the child at the age of 12-16 years.
The technical result of the method is to increase the informative value of clinical and laboratory examination of the child through the use of study the ratio of the concentration of immunoglobulin A concentration of immunoglobulin G in oral fluid dynamics (14 days) to identify among children aged 12-16 years group risk reduction level of health.
The implementation of the invention
The claimed technical�th result is achieved by defining in the oral fluid of a child aged 12-16 concentrations of immunoglobulin A and the concentration of immunoglobulin G twice the difference in 14 days and the mouth for 15 minutes prior to collection of oral fluid is flushed with a solution of sodium chloride 0.9%, in the case of reducing the concentration ratio of antibody And the concentration of immunoglobulin G in the second study, 1.4 times or more the child is considered at risk of reducing the level of health. The intake of oral fluid is an hour after a meal, thus reducing the impact of hypersalivation due to the anticipation of food intake or eating food.
Just in the manner we examined 90 children aged 12-16 years, 70 of them children II health groups (children with a low resistance index in the frequency of acute diseases with risk of transition in III group of health - chronic bronchopulmonary pathology (study group) and 20 children I health group (control group).
The material for the study served as oral fluid collected after pre-rinsing the mouth with a solution of sodium chloride 0.9% over 15 minutes before collecting the oral fluid. Were oral fluid intake in a volume of 1 ml in special vials for collection and centrifugation of venous blood. The tubes were signed and were transported to the laboratory in cooling the cold box. In the laboratory were conducted to determine the concentration of immunoglobulin A and immunoglobulin G. Then calculated related�e concentration of immunoglobulin A concentration of immunoglobulin G.
After 14 days, the examination was repeated.
In the case of reducing the concentration ratio of antibody And the concentration of immunoglobulin G in the second study, 1.4 times or more the child is considered at risk of reducing the level of health.
The novelty of the proposed method lies in the fact that the first proposed to predict the risk of reducing the level of health of a child aged 12-16 directly exploring non-invasive method the intensity of the immune response in the dynamics. The method is widely available, cheap, allows the use of standard test systems without any additional modifications. The distinguishing features of the method:
- the child twice, with a difference of 14 days, determine the concentration of immunoglobulin A and the concentration of immunoglobulin G in oral fluid;
- the research is conducted in an hour after eating;
- in 15 minutes prior to collection of oral fluid the mouth is washed with sodium chloride solution of 0.9%;
- when you reduce the ratio of the concentration of immunoglobulin A concentration of immunoglobulin G in the second study, 1.4 times or more the child is considered at risk of reducing the level of health.
The proposed method is illustrated by the following examples.
Clinical example No. 1.
The child, at the age of 13. A child by her third pregnancy, first birth. Breastfeeding up to 8 m�S. Attended kindergarten for 3 years. Currently enrolled in secondary school. The study of oral fluid by the proposed method. Identified reducing the concentration ratio of antibody And the concentration of immunoglobulin G in the second study in 0.3 times the risk of decrease of a level of health missing. The child was observed during the year. Last year it was 2 cases of acute respiratory infections. The forecast was confirmed.
Clinical example No. 2.
The child, at the age of 14. Child from first pregnancy. Artificial feeding from birth. Kindergarten did not attend. The study of oral fluid by the proposed method. Identified reducing the concentration ratio of antibody And the concentration of immunoglobulin G in the second study, 1.8 times revealed a high probability of the risk reduction level of health. The child was observed during the year. During the year there were 7 cases of acute respiratory disease, pneumonia. The forecast was confirmed.
A method of predicting the risk of reducing the level of health of the child aged 12-16, which consists in the study of the oral fluid of a child with the determination of the concentration of immunoglobulin A and immunoglobulin G, characterized in that the research is conducted in an hour after meal, twice with a difference of 14 days, the mouth for 15 minutes prior to collection of oral fluid by promvestsistem of sodium chloride 0,9%, calculated the ratio of the concentration of immunoglobulin A concentration of immunoglobulin G, in the case of reducing the concentration ratio of antibody And the concentration of immunoglobulin G in the second study, 1.4 times or more the child is considered at risk of reducing the level of health.
SUBSTANCE: invention refers to medicine, namely to gynaecology, and concerns a method for the prediction of endometrial hyperplasia in the patients with hysteromyoma following uterine artery embolisation (UAE). Substance of the method: all the patients undergo a pre-UEA diagnostic and therapeutic uterine scrapping; 6 months after the UAE, an endometrial pipelle biopsy followed by a pathomorphological study of the biopsy material is conducted. If the study shows no pathology, the biopsy materials are analysed by means of an immune histochemical study using anti-Ki-67 monoclononal antibodies; a percentage of Ki-67 proliferation marker in the uterine gland epithelium (coefficient A) and in the endometrial stroma (coefficient B) is determined; a prognostic index D1 is calculated in initial(pre-UEA) non-atypical hyperplasia, and a prognostic index D2 - in initial (pre-UEA) endometrium in the late proliferation phase: D1=A*0.05-0.74 and D2=B*0.02-0.3. If D1 is 0 or more, recurrent endometrial hyperplasia is predicted, whereas D1 less than 0 enables stating a low risk of recurrent endometrial hyperplasia. If D2 is more than 0, a low risk of developing endometrial hyperplasia is stated, whereas D2 of 0 or less shows developing endometrial hyperplasia.
EFFECT: presented method enables the high-accuracy prediction of the post-UEA endometrial hyperplasia that makes it possible to prescribe the preventive hormone therapy in due time.
SUBSTANCE: claimed invention relates to medicine, namely to immunology, and can be applied for detection of contaminants in glucose polymers. For this purpose analysis of inflammatory response is carried out in vitro with application of cell line, which represents cell line of either macrophages or differentiable in macrophages, or cell, expressing one or several toll-like receptors (TLR) or NOD-like receptors, selected from TLR2, TLR4 and NOD2. Analysis contains the following stages: (a) placing macrophages in presence of preparation of glucose polymers, which can contain anti-inflammatory contaminants, and measuring cytokine RANTES production, with production of RANTES cytokine indicating to the fact that preparation contains contaminants capable of initiating inflammatory reaction, and (b) placing cell line, which makes it possible to detect activity of innate immunity receptor or several receptors of innate immunity, selected from TLR2 and NOD2, in presence of preparation and detection of signal of reporter gene, bound to said receptor, with detection of said activity or said signal indicates presence in preparation of contaminant, which represents receptor agonist.
EFFECT: application of claimed method makes it possible to detect contaminants of glucose polymers, and addition of components, such as MDP or LPS, in tested sample makes it possible to act synergistically with contaminants, which increases sensitivity and reduces threshold of detection, with synergetic response being registered for RANTES.
28 cl, 5 ex, 23 dwg, 5 tbl
SUBSTANCE: peripheral leukocyte blood values before and after a loading test are measured with the use of the gradual submaximal exercise. The method involves the differentiated recovery of different types of leukocytes from the blood to produce a preparation; a total T-cell (Tt) and active T-cell (Ta) count is determined; a Tt/Ta ratio is derived; mononuclear cells are incubated with granulocytes; a granulocyte-binding lymphocyte index (GLI) is determined in accordance with a granulocyte rosette formation (GRL - contact bound to three granulocytes) to granulocyte contact lymphocytes (GCL - contact bound to one granulocyte) ratio in the preparation; leukocyte indices: lymphocyte index (LI), immune reactivity index (IRI), adaptation index, ("CПHP") are determined; immune functional state adaptation coefficients (K) are derived for each value. Total immune functional body state TIFBS is calculated by formula TIFBS = K"спнр"+Kli+Kiri+Kgli+K"итл" before the exercise - TIFBS1 and after the exercise - TIFBS2. A specific immune functional state coefficient SIFSC is calculated by formula SIFSC = (TIFBS1+TIFBS2)/5, and a level of the immune functional SIFSC reserve is determined. If the SIFSC values are above +1.0, the level of the immune functional reserve is considered to be optimal; the SIFSC values falling within the range of 0 to 1.0 show the satisfactory reserve, whereas the SIFSC values below 0 shows the unsatisfactory reserve.
EFFECT: method enables assessing the functional body reserves with the use of combined characteristics including the adaptation body potential, immune reactivity and immune cell interaction.
SUBSTANCE: DNA is recovered from peripheral venous blood. Genetic polymorphisms of tumour necrosis factor α (-308 G/A TNFα), tumour necrosis factor 1 receptor (+36 A/G TNFR1), interferon-inducible T-cell chemoattractant (A/G I-TAC), interleukin 1A (-889 C/T IL-1A), lymphotoxin α (+250 A/G Ltα) are typed by polymerase chain reaction. A high risk of developing hyperplastic processes of endometrium is predicted if detecting a combination of alleles -308 G TNFα, +36 A TNFR1, A I-TAC, -889 T IL-1A and/or a combination of alleles +36 A TNFR1, A I-TAC, -889 T IL-1A and/or a combination of alleles -308 G TNFα,+250 G Ltα, -889 T IL-1A.
EFFECT: higher accuracy.
2 dwg, 1 tbl, 1 ex
SUBSTANCE: chronic infectious-inflammatory diseases (CIIDs) are diagnosed. Clinical blood analysis and bacteriological tests are conducted. A sensibilisation index (SI) and an immune responsiveness index (IRI) are calculated; total microbial count per 1 m3 of the working space air is measured, and the total microbial number (TMN) is derived. If the TMN is less than 500 CFU/m3 with no CIIDs diagnosed accompanied by the SI of less than 1.08 standard units and the IRI of less than 13 standard units, the immunoassay is considered to be inadvisable. If the TMN falls within the range of 500-2,500 CFU/m3 with one CIID diagnosed accompanied by the SI from 1.08 to 1.3 standard units and the IRI from 13.1 to 15.7 standard units, the immunoassay with the first-level tests seems advisable. Whereas the TMN exceeding 2,500 CFU/m3 with at least two CIIDs accompanied by the SI of 1.4-1.5 standard units and the IRI of 15.8-18.3 standard units, the immunoassay with the second-level tests is thought expedient.
EFFECT: invention enables detecting the workers in need of further examination for the purpose of timely immune correction in the setting of mass routine examinations.
1 tbl, 3 ex
SUBSTANCE: DNA from peripheral venous blood is extracted. An analysis of a combination of genetic versions of polymorphous markers of genes of cytokines of the gene regulator of the activity of normal expression and secretion of T-cells (-403 G/A RANTES), macrophage protein -1β (+1931 A/T MIP 1β), factor of stromal cells (-801 G/A SDF1), interleukin -1 (-511 C/T IL-1B), monocyte chemoattractant protein -1 (C/G MCP-1), interleukin -4 (-590 C/T IL-4) is performed. An increased risk of development of a combination of uterine myoma with endometriosis and hyperplastic processes of the endometrium is predicted if the combination of alleles 403 A RANTES, G MCP-1,+1931 A MIP 1β, -590 C IL-4 or the combination of alleles -403 A RANTES,+1931 A MIP 1β, -801 G SDF1, -511 C IL-1B is identified.
EFFECT: application of the claimed method makes it possible to detect a group of patients with a risk of developing a combination of proliferative reproductive system diseases, which makes it possible to prescribe an adequate therapy to prevent further progressing of the diseases.
3 dwg, 2 ex
SUBSTANCE: invention deals with method of predicting level of arterial pressure in women of Russian nationality, born in Central Black Earth region of Russia. Method includes separation of DNA from lymphocytes of peripheral venous blood and analysis of genetic polymorphisms. +46G/A ADRB2 and 4a/4b eNOS by method of polymerase chain reaction Level of systolic arterial pressure in women in late pregnancy is predicted by results of multiple regression equation of the following type: Y1=15,455+2,544x1+9,946x2+0,736x3+4,716x4+0,185x5, where x1 is genetic variant in locus - 4a/4b eNOS, namely 4b4b=1; 4a4b=2; 4a4a=3; x2 is presence of preeclampsia in relatives: yes=0, no=1; x3 is level of systolic arterial pressure before pregnancy, mm Hg; x4 is presence of cardiovascular system pathology: yes=0, no=1; x5 is woman's weight before pregnancy, kg Level of diastolic arterial pressure in women in late pregnancy is predicted, for which purpose multiple regression equation of the following type is used: Y2=14,200+7,768x1-2,877x2+7,500x3+0,414x4+3,668x5, where x1 is genetic variant in locus - 4a4b eNOS, namely 4b4b+4a4b=1, 4a4a=0; x2 is genetic variant in locus +46G/A ADRB2, namely GG+GA=1, AA=0; x3 is presence of preeclapsia in relatives:yes=0, no=1; x4 is systolic arterial pressure before pregnancy, mm Hg; x5 is presence of cardiovascular system pathology: yes=0, no=1.
EFFECT: invention makes it possible to realise early prediction of increase of arterial pressure level in women in late pregnancy, will make it possible to form of women at the stage of pregravidal preparation and at early terms of pregnancy groups of high risk of developing hypertension in late pregnancy, as well as realise required therapeutic-preventive measures aimed at prevention of development of said pregnancy complication in due time.
2 dwg, 2 tbl, 1 ex
SUBSTANCE: method is based on contacting a membrane test strip with an analysed fluid sample and initiating thereby a motion along the test strip membranes of reagents being parts of the sample or coating the membrane, and forming the immune complexes to be detected in the course of reactions in the membrane pores or on the surface thereof. A distinguishing feature of the presented method for antigen detection is that the test strip is coated additionally within the test sample contact area with a certain amount of specific antibodies, which react to the detected antigen expected to be found in the sample, when a fluid front moves and block a certain number of binding sites. The number of the coating free antibodies is specified so that the low content thereof in the analysed sample being of no diagnostic importance ensures blocking the binding sites completely that prevents the antigen from binding in the analysed area of the test strip and from developing a destructive staining in the analysed area.
EFFECT: presented approach enables reliable diagnosing based on the detection results of the antigens of gastrointestinal disorders, avoiding the achievement of positive test results for the low-antigen samples, which testifies to no development of the disease in an individual.
1 tbl, 2 ex
SUBSTANCE: invention relates to field of medicine, in particular hepatology and infectious diseases, and can be used for determination of stage of fibrous process in monitoring of patients with chronic hepatitis C. To realise method levels of blood serum cytokins are determined in patients with chronic hepatitis C with diagnosed by means of biopsy or other non-invasive method stage of fibrous process 2 times a year, with further calculation of cytokine profile integral index (CPII) on their basis, at initial stage F0 growth of CPII higher than -8 testifies to debut of fibrous changes in liver (transition to stage F1), at initial stage F1, drop of CPII below -10 testifies to transition to stage F2, at initial stage F2 growth of CPII higher than -3 testifies to transition of fibrosis to stage F3, at initial stage F3 drop of CPII below -3 testifies to development of cirrhosis.
EFFECT: determination of stage of fibrous process in monitoring of patients with chronic hepatitis C.
3 ex, 1 tbl, 5 dwg
SUBSTANCE: invention relates to medicine, namely to a method of predicting spontaneous onset of pregnancy in women with I and II stage of external genital endometriosis. The essence of the invention consists in the fact that before treatment in peripheral blood of women with infertility, associated with I and II stage of external genital endometriosis determined is a relative quantity of IL- IL-1β + monocytes, and if the value of the said index is 50.0% and higher in the monocytic gate the spontaneous onset of pregnancy within a year after carrying out the surgical treatment of endometriosis is predicted.
EFFECT: application of the claimed method makes it possible to predict with high accuracy the spontaneous onset of pregnancy in the women with infertility in case of I and II stage of external genital endometriosis within a year after therapeutic laparoscopy, which makes it possible to select optimal tactics of the patients' management and estimate the necessity of administering them methods of assisted reproductive technologies.
1 tbl, 3 ex
FIELD: medicine, ophthalmology.
SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.
EFFECT: higher accuracy of prediction.
FIELD: medicine, medicinal microbiology.
SUBSTANCE: method involves growing microorganism culture to be studied in solid nutrient medium followed by preparing microbial suspension and its incubation in the presence of lactoferrin. Control sample is prepared in parallel series. Control and experimental samples are incubated, supernatant is removed from bacterial cells and lactoferrin concentration is determined in supernatant of experimental and control sample by immunoenzyme analysis. Then anti-lactoferrin activity is calculated by difference of concentrations of residual lactoferrin in experimental and control samples. This method provides enhancing the sensitivity and precision in carrying out the quantitative evaluation of anti-lactoferrin activity in broad spectrum of microorganisms that is urgent in diagnosis and prognosis of diseases with bacterial etiology. Invention can be used in determination of persistent indices of microorganisms for assay of their etiological significance in pathological processes.
EFFECT: improved assay method.
3 tbl, 3 ex
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.
FIELD: medicine, cardiology.
SUBSTANCE: in peripheral blood one should detect the level of CD95(+) and CD16(+) neutrophilic granulocytes and at combination of increased level of CD95(+) neutrophilic granulocytes by 4 times and more and CD16(+) neutrophilic granulocytes by 0.6 times against the norm with ECG signs of myocardial infarction one should predict lethal result of large-focal myocardial infarction.
EFFECT: higher accuracy of prediction.
FIELD: medicine, parasitology.
SUBSTANCE: one should carry out immunoenzymatic assay to detect diagnostic optic density and that of labeled immune complex in a plot's hole with tested serum measured in conventional units at wave length being 492 nm. One should calculate coefficient of antibodies concentration measured in conventional units by the following formula: CAC = (Odtsh - Odd) x 100, where CAC - coefficient of antibodies concentration, Odtsh - optic density of the hole with tested serum, Odd - diagnostic value of optic density, 100 - coefficient of serumal dilution. By CAC value one should detect the titer of antibodies to Lamblia intestinalis antigens to interpret results of the trial. The method enables to study the dynamics of disease flow.
EFFECT: higher efficiency and accuracy of diagnostics.
1 ex, 1 tbl
SUBSTANCE: the present innovation deals with studying and treating diseases of inflammatory, autoimmune and degenerative genesis. One should perform sampling of heparinized blood followed by its sedimentation to obtain blood plasma with leukocytes and centrifuging to isolate the latter which are washed against erythrocytic and serumal admixtures, and, also, it deals with calculating the number of cells in samples out of leukocytic suspension after incubation (B) for 1.5 h at 37 C in holes of plastic microplotting board, out of leukocytic suspension one should additionally prepare two samples, one should be applied to calculate total number of leukocytes before incubation (A), the second sample undergoes incubation at the same mode at addition of autoserum to calculate the number of cells remained after incubation (C). One should state upon adhesive properties of leukocytes by the index of spontaneous adhesion (D), where D=(A-B)/B.100%, and effect for enhanced cellular adhesion under the impact of autoserum should be detected by the value of K=(B-C)/C.100% at K ≥ 30%, where B - C - the number of cells undergone additional adhesion after addition of autoserum. The present innovation widens functional possibilities of the suggested method due to obtaining additional values depicting adhesive properties of blood leukocytes.
EFFECT: higher accuracy of detection.
FIELD: medicine, immunology.
SUBSTANCE: one should carry out reaction of blast-transformation, detect proliferation of T-lymphocytes activated with antibodies to CD3 in the presence of interleukin-7 (ACT IL-7) and in the presence of interleukin-7 and dexametazone (ACT IL-7 D), calculate the index for dexametazone action as the ratio of ACT IL-7 to ACT IL-7 D, moreover, the value of dexametazone action index being above 1.2 indicates increased production of cytokins that suppress T-lymphocytes in neonatals. The method enables to detect functional defect of immune system that characterizes neonatal period.
EFFECT: higher efficiency of detection.
SUBSTANCE: method involves measuring forced exhalation volume per 1 s (FEV1) in l, full right ventricle evacuation time (RVE) in ms and angiotensin II value (AII) in ng/l. Discriminant relationship is built as D=0.504·RVE+3.038·FEV1 - 2.0·AII. D being less than 83.88, pulmonary hypertension occurrence is predicted within 1 year. D being equal to or greater than 83.88, no pulmonary hypertension is predicted to occur.
EFFECT: enhanced accuracy of prediction.
FIELD: medicine, medicinal immunology.
SUBSTANCE: method involves determination of heterophilic antibodies in human serum blood by the Paul-Bunnel's method relatively the level of circulating immune complexes, complement-activating properties of heterophilic antibodies by incubation of standardized ram erythrocytes with 0.8% serum for 30 ± 5 min and the following measurement of the erythrocytes lysis degree. The measurement of the effector function coefficient of heterophilic antibodies is carried out by the complement system Keff.f.h.a.-c.s. by the formula: Keff.f.h.a.-c.s. = Y/Tg.a. wherein Y means a lysis degree, %; Tg.a. means a reverse titer of heterophilic antibodies to ram erythrocytes. The damage assay is carried out by comparison of the immune status with the relative level of circulating immune complexes in serum. Method provides detection of preclinic from of immunodeficiency and autoimmune diseases that opens the possibility for their prophylaxis at most early stages of development. Invention can be used for assay of damage in the immune status in human serum blood.
EFFECT: improved method for assay.
5 tbl, 1 ex
SUBSTANCE: method involves concurrently examining anti-inflammatory IL-4 level in blood serum and lacrimal fluid. The value being within the limits of 60-70 pg/l in blood serum and 5-15 pg/l in lacrimal fluid, disease prognosis is considered to be unfavorable. The IL-4 concentration being within the limits of 90-100 pg/l in blood serum and 20-30 pg/l in lacrimal fluid, disease prognosis is considered to be favorable.
EFFECT: high accuracy of diagnosis.