Method for determining female serum cytotoxicity to male monoclear cells
SUBSTANCE: method involves determining female serum cytotoxicity to male lymphocytes, including a combined culture with reference male and analysed female serum in a 96-well tray in the presence of the nutrient medium RPMI 1640 in a CO2 incubator. One day later, lymphocytes are counted in the well in a Goryaev's chamber with the male (reference) and female (analysed) serum. That is followed by determining a cytotoxic index (CI), which represents a quotient of the analysed cell count and the reference cell count. The normal cytotoxic index makes approximately 0.7 and less.
EFFECT: invention enables studying the responses of female humoral immune factors to male antigens and evaluating a risk of miscarriage, early spontaneous abortions and missed miscarriages.
The increase in the number of infertile marriages registered in Russia and other countries, dictates the need to find modern methods of diagnosis and treatment of infertility, especially in cases where the reason it is not installed. One of the causes of infertility can be a high degree of tissue compatibility of the spouses and, as a consequence, a weak immune response and low production of protective factors. As a result of an immune response to the antigens responsible for the generation of specific cytotoxic antibodies against antigens of the father. This is a blocking antibody IgG presents. They bind to Fc receptors on lymphocytes (NK-cells) and, thus, prevent the accumulation of activated NK cells with high cytotoxic activity.
It is shown that this mechanism works most efficiently when the maximum differences of the spouses to the antigens of major histocompatibility complex (HLA antigens). Weak immune response and insufficient production of protective factors leads to the development of early gestational complications - spontaneous miscarriage or missed abortion.
As a result of an immune response to HLA antigens are produced by specific protective factors: cytotoxic antibodies against antigens of the father (ARSA - antipaternal cytotoxic antibodies); Ab2 anti-anti HLA antibodies (Ab2-anti-anti HLA antibodies); antibodies that block mixed cult�ru lymphocytes (MLR-Bf - mixed lymphocyte reaction blocking factor) [1, 2].
Laboratory tests to confirm the diagnosis alloimmune nature of miscarriage include HLA typing, the definition of maternal lymphocytotoxicity antibodies against the father, a blocking antibody to a mixed culture of cells (SCR), etc.
That these laboratory studies may also be useful in the diagnosis of infertility, demonstrated by the fact that 85% of pregnancies are terminated before the fact of their establishment. It is assumed that recurrent pregnancy loss and infertility are manifestations of existing immune disorders [3-6].
The invention relates to medicine, in particular to methods of determining the state of the immune system, and relates to a method of determining cytotoxicity of the serum female to male lymphocytes.
Analogous to the claimed method is a Method for quantifying the concentration of antibodies specific to the antigen endometrial stromal cells human tissue, in biological fluids of a person, containing specific antibodies" .
Method is to use the traditional enzyme-linked immunosorbent assay (ELISA) and includes chemical binding of the antigen with the surface of the microplate, followed by biological fluid containing antibodies, micro�Lancet, incubation, carrying out the color reaction and spectrophotometric evaluation of indicators of the color reaction. This produces a target antigen, exposing the enzymatic hydrolysis of the samples of normal human tissues containing stromal cells of the endometrium. The enzymatic hydrolysis reaction is stopped by addition of a chelating agent. Received a mixed cell suspension is subjected to centrifugation in a continuous density gradient, resulting in isolated stromal cells. Microsomal fraction of stromal cells used as target antigen in the selected dilutions covalently associated with the surface of the microplate wells, then to make a series of holes in predetermined dilutions of the calibration material, prepared on the basis of the standard drug is specific to the target antigen of antibodies, in another series of holes - measured sample of a biological liquid and once the traditional TYPHUS by spectrophotometric indices build a calibration curve and determine the concentration of specific antibodies.
The disadvantage of analog is the complexity of technology the cells of normal human tissues to identify the antigen, proper calibration, obtaining the necessary components and equipment setting reaction.
As� prototype, the authors propose a Method of determining the blocking effect of autologous serum .
The method consists in the fact that the lymphocytes were incubated in medium with addition of phytohemagglutinin, in the environment with the addition of phytohemagglutinin and autologous serum in the medium with the addition of phytohemagglutinin and polerowanej AB-serum and in the absence of additives, followed by the addition of FITC-labeled antibodies to CD69. Further assessment of the fluorescence in each sample and determine the inhibitory effect on the proposed formula.
The disadvantages of the prototype are the complexity of implementation, expensive materials and equipment, lack of trained this technique personnel.
The problem to be solved by the claimed invention is to simplify the methodology for the determination of antibodies (blocking factors) serum female to male lymphocytes, reducing the time of carrying out the method and to assess the possible risk of miscarriage.
This problem is solved due to the fact that the claimed method of determining the cytotoxicity of the serum female to male lymphocytes can be performed proposed by the authors in the following manner.
A collection of blood in women is 10-20 day cycle. The blood in an amount of 5 ml is taken from women in a single tube blood collection tubes with red cap on the serum. In men taking blood 2 vials 5 ml: one with a red cover on the serum, the other with a green tube with �eparina for isolation of lymphocytes.
Tubes of blood with red cap mark put in a separate stand for 10 min in a thermostat at 37°C (at room temperature 20 min), then 20 min in the fridge. Then centrifugum 3 thousand rpm for 10 min. Serum was collected in a sterile tube.
For receiving male blood lymphocytes layered on ficol-verografin (density 1.077) (LTD. Company Panaco" Moscow) and centrifugum 20 min at 3 thousand rpm the resulting interphase lymphocytes washed three times, first in buffered physical solution (0.01 M, pH=7,4), then in a nutrient medium RPMI 1640. Determine the number of cells in 1 ml by count in the camera Goryaeva and calculates the concentration so that 1 ml was 2 million lymphocytes.
In 96-well sterile tablet insertion in the 1st and 2nd hole 100 µl of cell suspension, 20 μl of RPMI medium 1640, 1 insertion hole 30 ál serum women (experience), the 2nd - 30 ál serum men (control), resuspended and set for 24 hours at 37°C in CO2an incubator.
A day believe the number of lymphocytes in the presence of female Sivaratri (experience) and male (control).
The cytotoxic index expressing the number obtained as a result of the private from dividing the number of cells in the experiment on the number of lymphocytes in the control. Normal cytotoxic index equal to about 0.7 and below.
It �the means, in women's serum contains factors that inhibit the proliferation of male lymphocytes in pregnancy and there will be no attack of the killer cells to the antigens of the fetus.
The technical result provided by the above combination of features is the reliability, efficiency, speed of execution and efficiency of determining the level of antibodies (blocking factors) serum female to male lymphocytes, speeding up the diagnosis.
The proposed method will provide a fast, a day from the beginning of studies, economical and efficient to undertake a study of the reaction of humoral factors of the immune system of the female body to male antigens and to assess the risk of miscarriage, early pregnancy loss, non-developing pregnancy, etc.
The method is illustrated by the following example.
We examined 36 women who had pregnancy and childbirth proceeded without physiological abnormalities, 32 women with recurrent miscarriage of unknown origin.
The cytotoxic index of 87% women, the first group was 0,7±0,2, 13% - 0,5±0,26.
The cytotoxic index in 79% of women of the second group with recurrent miscarriage of unknown origin was 2,5±0,7, 21% - 1,0±0,6.
Method for determining cytotoxicity of the serum female to male lymphocytes will allow to assess the reaction of the immune system women�s to male antigens and predict possible risks of miscarriage. This study gives the opportunity to take timely corrective action by assigning therapeutic interventions. To them currently include the conduct of alloimmunization (Permission to use FS No. 2009/179 from 2.07.2009 G. ) and the use of the drug normal immunoglobulin for intravenous administration [7-9].
Thus, the proposed method will enable rapid diagnosis of immune disorders in women, and as a result will increase the birth rate.
1. Pandey M. K., Agrawal S. Induction of MRL-Bf and protection of fetal loss: a current double blind randomized trial of paternal lymphocyte immunization for women with raccurent spontaneous abortion. Int Immunopharmacol 2004; 4:289-298.
2. Ito K., Tanaka T., Tsutsumi N. Possible mechanisms of immunotherapy for maintaining pregnancy in recurrent spontaneous aborters: analysis of antiidiotype antibodies directed against autologous T-cell receptors. Hum Reprod 1999; 14:650-655.
3. Bezkorovainy T. C, I. V. Poltavets, Twin E. A., Wasserman N. N., Tverskaya CM., Polyakov L. V. the Role of class II antigens of major histocompatibility complex in habitual miscarriage. Problems of reproduction 2006; 12:2.46-54.
4. Khonina N. And., Tikhonova M. A., Dzutseva I. B., Pasman N. M. Ostanin A. A., Chernykh E. R. Immune dysfunction in women with unexplained infertility. Honey. immunology 2010; 12:6:511-520.
5. Yamada N., Atsumi, T., Kato, E. N. Prevalence of diverse antiphospholipid antibodies in women with recurrent spontaneous abortion. Fertil Steril 2003; 80:1276-1278.
6. Koyama M., Saji F., Takahashi S., Takemura M. Probabilistic assessmen of the HLA-sharing of recurrent spontaneous abortion couples in Japanese population. Tissue antigen 2010; 37:211-217.
7. Chernyshov V. N., Sudoma I. A., don, B. V., Kostiuczyk A. A., Masliy Y. the Value of the increased cytotoxicity of natural killer cells during repeated failure of in vitro fertilization. Phys. The Academy of medical Sciences of Ukraine, 2010, vol. 16, No. 2, pp. 288-298.
8. Markov S. A., Men'shikov V. I., Bedulev L. V., ] N.N., Kazakov I. G., Veretennikova K. G. Clinical and diagnostic value of metocolopramide immune response of the mother to the antigens of the father and of the establishment of tolerance in pregnancy. Bulletin of Udmurt University. 2011. Vol. 4. Biology, earth science, Physiological studies, pp. 102-106.
9. Khonina N. A., Broitman E. V., Shevela E. Y., Pasman N. M., Chernykh E. R.. Mixed lymptocyte reaction blocking factors (MRL-bf) as potential biomarker for indication and efficacy of paternal lymphocyte immunization in recurrent spontaneous abortion//Arch. Cynecol. Obstet-2013. - Vol. 288. - P. 933-937.
10. Mihnina E. A., Komarov E. K., Khokhlov p. P. Method for the quantitative determination of the concentration of antibodies specific to the antigen endometrial stromal cells human tissue, in biological fluids of a person, containing specific antibodies. Patent No. 2303267 dated 08.06.2005.
11. Sukhikh G. T., Vanko L. V., Sidelnikov V. M., Nikolaeva M. A., Ziganshin M. M., Krechetova L. V. Method of determination of the blocking effect of autologous serum. Patent No. 2396566 from 29.04.2009.
Method for determining cytotoxicity of the serum female to male lymphocytes, including their together�Noah cultivation with control male and investigated female serum in the wells of 96-well plates in the presence of a nutrient medium RPMI 1640 in the CO 2incubator during the day, followed by counting the number of lymphocytes in the cell Goryaeva and determination of cytotoxic index, expressed in private from dividing the number of cells in the experiment on the number of cells in the control at the normal rate of about 0.7 and below.
SUBSTANCE: invention relates to the field of medicine and is intended for the diagnostics of an occlusive affection of vessels in patients with cardiovascular diseases. The age, the level of total homocysteine in blood, the presence of C677T polymorphism mutations in the gene of methyletetrahydrofolate reductase, factor V gene G1691A Leiden mutation, 675 4G/5G polymorphism mutations in the gene of the plasminogen activator inhibitor type I are determined in the patient, after which a value of the discriminant function is calculated by formula. In case of D>0, the presence of the occlusive affection of vessels is diagnosed, if D<0, the absence is diagnosed.
EFFECT: invention provides the effective method for the diagnostics of the occlusive affection of vessels in the patients with cardiovascular diseases.
SUBSTANCE: method includes the determination of an inhibiting impact of a solution of highly-toxic anticholinesterase poison on cholinesterase activity, represented by acetylcholinesterase of human blood erythrocytes, in the presence and in the absence of a substrate, represented by acetylthiocholiniodide. The inhibiting impact of the solution of highly-toxic anticholinesterase poison on the activity of acetylcholinesterase of the human blood erythrocytes is determined first in the presence of the substrate, and then in its absence. An analysed sample is preliminarily diluted by 10 or 100 times to obtain the inhibiting effect on acetylcholinesterase of the human blood erythrocytes in the presence and in the absence of the substrate within 25-75%. After that, the value of the protective effect of acetylthiocholiniodide and the obtained calculated value is compared with table values, determined experimentally in advance for each particular highly-toxic anticholinesterase poison. If the calculated values coincide with the table values, a conclusion about the presence of a respective highly-toxic anticholinesterase poison in the analysed sample is made.
EFFECT: increase of safety.
SUBSTANCE: technique is implemented by biochemical blood analysis followed by determining a diagnostic index by formula: D=X1×K1+X2×K2+X3×K3+X4×K4+const, wherein the values X are described by the biological parameters: X1 is the ceruloplasmin concentration, g/l; X2 is the creatinine concentration, mcmole/l; X3 is the total protein concentration, g/l; X4 is the albumin concentration, g/l; K1, K2, K3; K4 are coefficients: K1=10.4, K2=-0.04, K3=-0.10, K4=0.26, const=-6.84. If D is more than 0, the uncomplicated gestation course is stated with certainty. If D is less than 0, the threatening miscarriage is predicted.
EFFECT: method enables the timely detection of a group of the threatening miscarriage risk, and enables the correct classification.
SUBSTANCE: group of inventions relate to medicine and deals with method of diagnosing neurodegenerative disease in individual, including the following stages (i) determination of one or several parameters, selected from group, consisting of 3ab40 or value of calculated parameter, selected from group, consisting of 2ab40+3ab40, 2ab40+3ab40+2ab42+3ab42 and 1ab40+2ab40+1ab42+2ab42; (ii) comparison of parameter value with standard value, corresponding to value of said parameter in standard sample; and (iii) diagnostics of neurodegenerative disease, in case if increase of parameter value in comparison with standard value is observed. Group of inventions also deals with method of detecting stage, preceding neurodegenerative disease, method of differentiating neurodegenerative disease from stage, preceding said neurodegenerative disease.
EFFECT: group of inventions provide high sensitivity and specificity of detection methods.
13 cl, 12 ex, 14 dwg, 12 tbl
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of acquired myopia in school children. The substance of the method consists in measuring blood haemoglobin concentrations in 6-8-year-old school children to detect haemoglobin deficiency as shown by the difference of an optimum haemoglobin concentration specific for the above age and an actual haemoglobin concentration in a child. If observing no haemoglobin deficiency at the age of 6-8 years old, a low risk of acquired myopia is predicted. The haemoglobin deficiency to 1.7 g/l enables predicting a risk of acquired low myopia. If the haemoglobin deficiency is 1.7 g/l and more, a high risk of progressive myopia to be developed into moderate or high myopia is predicted.
EFFECT: using the declared method enables developing the reliable and accessible method for the prediction of myopia in the 6-8-year-old children.
2 tbl, 1 dwg, 6 ex
SUBSTANCE: invention relates to method of diagnosing rheumatoid arthritis, method of determining therapeutic agent for treatment of rheumatoid arthritis and set for realisation of methods. Methods are characterised by the fact that include stage of measuring amount of talin in plasma or serum of animal subject. Said measurement is carried out, for instance, by immunologic method with application of antibody, binding with talin. If amount of talin is higher than its average value in control subject without rheumatoid arthritis, rheumatoid arthritis is diagnosed in subject. In case of reduction of talin amount after introduction of therapeutic agent in comparison with amount of talin before introduction, therapeutic effect is stated. Set in accordance with claimed invention contains solid-phase carrier, to which antibody, binding with talin, is attached.
EFFECT: increased efficiency of diagnostics.
9 cl, 4 tbl, 4 ex, 3 dwg
SUBSTANCE: invention relates to medicine, namely to a method of predicting a probability of reduction of the glomerular filtration rate (GFR) after 3 months of observation after aortocoronary bypass surgery without artificial blood circulation (ACBS without ABC). The essence of the method consists in the fact that the concentration of a kidney injury molecule of type 1 (KIM-1) is determined in blood serum, the ratio of the biomarker KIM-1 concentrations in two time points after 48 hours and 7 days after the operation is calculated and if its value is higher than 1.5, a conclusion about the probability of (GFR) reduction in the remote period after ACBS without ABC is made.
EFFECT: application of the claimed method makes it possible to predict the probability of the glomerular filtration rate (GFR) reduction after 3 months of observation after aortocoronary bypass surgery without artificial blood circulation in an efficient and accurate way.
1 tbl, 1 dwg, 1 ex
SUBSTANCE: early diagnostic technique for pulmonary thromboembolism consists in the fact that if the patient shows any clinical signs indicative of the probability of pulmonary thromboembolism, blood serum fibrin D-dimer is supposed to be measured; if the fibrin D-dimer is less than 0.5 mg/l, a suspected case of pulmonary thromboembolism is cleared of, whereat blood serum fibrin D-dimer of 0.5 mg/l and more requires measuring blood serum interleukin-8 additionally by enzyme immunoassay, and if the measured concentration is 21.3 pg/ml and more, pulmonary thromboembolism is diagnosed.
EFFECT: more accurate and informative diagnostics.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of biotechnology, namely to obtaining oligopeptide compounds, containing a motive, interacting with a proliferating cell nuclear antigen (PCNA) and can be used in medicine. The oligopeptide compound consists of 14-70 amino acids and contains. a PCNA-interacting motive, representing [K/R]-[F/Y/W]-[L/I/V/A]-[L/I/V/A]-[K/R], at least one signal sequence of nuclear localisation and at least one signal sequence of penetration into a cell, with the PCNA-interacting motive being located towards an N-end relative to the signal sequence.
EFFECT: invention makes it possible to carry out the efficient treatment of hyperproliferative disorders by the application of the oligopeptide compound in cyctostatic therapy or in radiotherapy as a sensitising substance.
34 cl, 6 dwg, 4 tbl, 8 ex
SUBSTANCE: on paramagnetic particles bearing the immobilised bacterial protein G of the family Streptococcus, with the blocked solution Denhardt-DNA the protein BoNT/A is adsorbed using specific highly affinity polyclonal antibodies. The formation of the protein complex BoNT/A is detected with a biotin-conjugated antibody by a noncovalent conjugate of DNA fragments with neutravidin. PCR amplification of the DNA matrix is carried out with the fluorescence detection of the signal in real time. Registration of the presence of BoNT/A in the samples under study is carried out by the change in the level of fluorescence compared to the control ones.
EFFECT: effective method of determining the presence of the compound.
5 dwg, 1 tbl, 5 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a high-sensitivity method for measuring the amount of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts. The high-sensitivity method for measuring the amount of glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts is characterised by the fact that a mixture of individual's blood plasma with methanol or ammonia water in the specific concentration is introduced into a solid phase having the reverse-phase distribution function and the anion exchange function; the solid phase is then washed with a cleaning fluid that is a single-component fluid or a mixed fluid of at least two components specified in a group containing water, alkali, alcohol and acetonitrile. That is followed by elution from the solid phase in acid alcohol specified in formic acid - methanol or formic acid - ethanol; that is followed by the stage of measuring glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts by liquid chromatography - mass spectrometry or liquid chromatography - mass spectrometry/mass spectrometry.
EFFECT: high-sensitivity method enables detecting and measuring the amounts of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts.
4 dwg, 17 tbl, 7 ex
FIELD: veterinary medicine.
SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.
EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.
SUBSTANCE: patient's peripheral venous blood is recovered to analyse genetic polymorphisms of coagulation factors VII 10976G/A FVII. A birth weight of a newborn of a woman delivering not for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6123.431-25.579x1+0.267x2+205.739x3, wherein y is an anticipated newborn's weight, x1 is a female's height in centimetres; x2 is an infant's weight at the previous delivery in grams, x3 is a genetic version of 10976G/A FVII locus with x3=1 for 10976 GG FVII genetic type, x3=2 for 10976 GA and 10976 AA FVII genetic types. A birth weight of a newborn of a woman delivering for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6278.037-21.739x1+232.170x2, wherein x1 is a female's height in centimetres; x2 is a genetic version of 10976G/A FVII locus with x2=1 for 10976 GG FVII locus, x2=2 for 10976 GA and 10976 AA FVII genetic types.
EFFECT: invention enables improving the prevention and treatment of the pregnancy complications.
2 tbl, 4 ex
SUBSTANCE: pre-operative fasting venous blood 1 ml is sampled at room temperature 20-24°C into an anticoagulant-free vacuum system (test tube). The test tubes are delivered in a sealed container at temperature 2-8°C for 2 hours to a laboratory for immunoenzyme assay and analysed to determine anti-thyroid stimulating hormone receptor antibodies. If the antibody level is 1.5 units/l or more, a thyroidectomy is performed, whereas the antibody level of less than 1.5 units/l requires performing a subtotal thyroid resection according to standard techniques.
EFFECT: invention enables reducing a probability of recurrent thyrotoxicosis in the patients after subtotal thyroid resection.
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of a risk of early microvascular complications in the children suffering from type 1 diabetes mellitus. The substance of the method consists in defining a duration of the diseases in years, the patient's age in years, a desquamated endothelial cell count, high-density lipoprotein cholesterol, total cholesterol, triglycerides, atherogenic index, glycohaemoglobin, average daily glycaemic level; making a linear regression analysis and calculating a risk ratio (R) of early microvascular complications in the children suffering from type 1 diabetes mellitus by formula. If the risk ratio is ≥1, the high risk of early microvascular complications during one year is predicted; the ratio < 1 shows the low risk of microvascular complications during one year.
EFFECT: using the declared method enables increasing the prediction accuracy of microvascular complications in the children with type 1 diabetes mellitus.
2 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine and can be used for patient's status monitoring. A method for setting a time-varying physiological parameter warning signal involves patient's controlled parameter monitoring, comparing the controlled parameter to an initial cut-off criterion, varying the cut-off criterion temporarily by a cut-off criterion of deterioration after the therapy, and then after a certain period of time, by the cut-off criterion after the administration. The time allowed involves comparing the controlled parameter to the cut-off criterion of deterioration, and after the time allowed - to the cut-off criterion after the administration. The warning signal is initiated in response to the controlled parameter of one or more initial cut-off criteria, the cut-off criterion of deterioration and the cut-off criterion after the administration. The group of inventions also refers to a machine-readable carrier with software for implementing the method and to a system for user warning on the controlled parameter variation.
EFFECT: group of inventions provides higher accuracy and rate of assessing the patient's status according to the conducted therapy.
15 cl, 6 dwg
SUBSTANCE: invention can be used for the purpose of the early prediction of cystic periventricular leukomalacia (PVL) in the newborns with very low (VLBW) or extremely low body weight (ELBW). Substance of the method: the newborns with VLBW and ELBW on the 3rd-7th day of life are examined to assess the perinatal medical history, namely the presence of chorioamnionitis and amniotic fluid nature, 5th minute Apgar score, the absence of prolonged artificial pulmonary ventilation, a severity of respiratory distress syndrome, the presence of pneumonia, sepsis, convulsive disorder, anaemia, laboratory signs of the systemic inflammatory reaction, average values of carbon dioxide, anionic bicarbonate and base deficiency in capillary blood, interleukine-6 and receptor interleukine-1 antagonist in venous blood serum. Each sign is assigned with a prognostic coefficient (PC). That is followed by determining total PC, and it is expected cystic PVL that is decided for if total PC is at least (+)9.5, whereas no cystic PVL is expected if total PC is (-)9.5 or less.
EFFECT: invention provides the early (for the first 7 days of life) prediction of cystic periventricular leukomalacia in the most susceptible category of patients that are children with very low and extremely low birth weight.
6 tbl, 5 ex
SUBSTANCE: interferon is measured in plasmocytoid dendrite cells at week 12 of the treatment. Its gain in adults and children twenty times as much as compared to the pre-treatment initial level, or 2.6×102 times as much in adults, and 1.2×102 as much as compared to the normal values, the stable positive antiviral treatment response is predicted.
EFFECT: method enables providing the more accurate prediction for selecting the optimum therapeutic approach in each patient.
SUBSTANCE: invention represents a method for assessing the mucosal immunity state of open cavities accompanying the prediction of the clinical course of infectious-inflammatory processes, characterised by the fact that having a pathogenetic factor established, degrees of microbiocoenosis disturbances of a specific biotope are recorded with the use of a complex of methods for estimating colonisation resistance factors, namely normal microflora, opportunistic microflora, immunoglobulins G, M, A, secretory immunoglobulin A and sc component; the mucosal immunity state is assessed according to the degree of microbiocoenosis disturbance, and the favourable outcome implying agent eradication or chronisation with agent persistence is predicted. The invention also refers to a method for correcting the infectious-inflammatory processes with the Kipferon® immunomodulator added.
EFFECT: increasing the prediction accuracy of the clinical course of the disease, extending the range of the method for correcting the infectious-inflammatory processes.
2 cl, 3 ex, 6 tbl, 1 dwg
SUBSTANCE: content of calcium and protein in oral liquid is determined before and after physical load, as well as a day after physical load. Recovery of content of calcium ions and protein in oral liquid after physical load to initial values is considered to be a criterion of total recovery of athlete's-volleyball player's organism, with evaluating time interval, required for said process.
EFFECT: invention makes it possible to determine reserve abilities of organism and its adaptability in athletes-volleyball players to physical load.
2 dwg, 4 tbl
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.