Method for prediction of newborn's weight taking into account polymorphic version of 10976 g/afvii locus
SUBSTANCE: patient's peripheral venous blood is recovered to analyse genetic polymorphisms of coagulation factors VII 10976G/A FVII. A birth weight of a newborn of a woman delivering not for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6123.431-25.579x1+0.267x2+205.739x3, wherein y is an anticipated newborn's weight, x1 is a female's height in centimetres; x2 is an infant's weight at the previous delivery in grams, x3 is a genetic version of 10976G/A FVII locus with x3=1 for 10976 GG FVII genetic type, x3=2 for 10976 GA and 10976 AA FVII genetic types. A birth weight of a newborn of a woman delivering for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6278.037-21.739x1+232.170x2, wherein x1 is a female's height in centimetres; x2 is a genetic version of 10976G/A FVII locus with x2=1 for 10976 GG FVII locus, x2=2 for 10976 GA and 10976 AA FVII genetic types.
EFFECT: invention enables improving the prevention and treatment of the pregnancy complications.
2 tbl, 4 ex
A method for predicting the weight of a newborn taking into account polymorphic locus 10976 G/AFVII
The invention relates to the field of medical diagnostics, can be used to predict the weight of a newborn taking into account polymorphic locus 10976 G/AFVII.
It is known that the child's weight at birth is of great importance for the subsequent development and affect his health in the future. Children born with low birth weight more likely to suffer obstetric complications, they are more likely to develop hypoxia and neurological disorders. Low birth weight is a characteristic feature of the syndrome of delayed fetal growth. [Serov V. N. Critical care in obstetrics: a guide for physicians /V. N. Serov, S. A. Markin. - Moscow: Medistat, 2003. - 704 p.; Rybkin, N. L. Premature infants: Feto-infantile losses, morbidity, hormonal nature of the adaptation period [Text]:author. dis.... Cand. honey. Sciences: 14.00.33 /N. Leonid Rybkin; The Cauldron. state med. Univ - Kazan, 2000. - 24 p.].
The syndrome of delayed fetal growth is multifactorial nature, the development of which are involved in maternal and fetal factors. Of great importance in the formation of placental insufficiency and, consequently, low weight at birth is given a hypercoagulable and hereditary thrombophilia, which happens� thrombosis of the vessels of the placental bed [Makatsaria, A. D. Thrombosis and thromboembolism in gynaecology and obstetrics hospital [Text]: molecular-genetic. Mechanisms and strategy for prevention of thromboembolic complications: a guide for physicians /A. D. Makatsaria, V. O. Bitsadze, S. V. Akinshina. - Moscow: Med. inform. Agency, 2007. - 1064 p.].
One of the key factors of the hemostatic system is some factor VII in here (factor VII coagulation), which is involved in extrinsic pathway activation of blood coagulation, but also able to convert factor IX to active factor Ha in the internal activation of coagulation, leading to the launch of the coagulation cascade [Okorokov, A. N. Diagnostics of diseases of internal organs [Text]: a guide for physicians: the 10 /A. N. Hams. - Moscow: Med. lit., 2001-2005. - Vol. 5: Diagnosis of diseases of the blood system. Diagnosis of kidney disease. - Moscow, 2003. - 512 p.: ill.]. The most significant mutation factor VII coagulation mutation is 10976G/A or Arg353Gln (R353Q), which is characterized by the substitution of guanine (G) adenine (A) at position 10976, causing the replacement of arginine (Arg) to glutamine (Gln) at position 353 of the protein chain. This mutation is associated with low activity factor VII coagulation in plasma. Heterozygous variant 10976G/A FVII (R/Q) leads to decreased activity of factor VII coagulation by 25%, and the presence of homozygous variant A/A FVII (Q/Q) - to reduce by about 50% �compared to carriers of the more commonly 10976G/G FVII (R/R). The prevalence in the European population is 10-20%. Option 10976G/G FVII (R/R) is an additional risk factor for vascular complications and pregnancy complications, and the presence of variants A/A FVII (Q/Q) and 10976G/A FVII (R/Q) significantly reduce the risk of coronary heart disease and myocardial infarction [Makatsaria A. D. Thrombosis and thromboembolism in gynaecology and obstetrics hospital [Text]: molecular-genetic. mechanisms and strategy for prevention of thromboembolic complications: a guide for physicians /A. D. Makatsaria, V. O. Bitsadze, S. V. Akinshina. - Moscow: Med. inform. Agency, 2007. - 1064 p.].
In examined medical research and the available patent literature, the authors found no way of predicting birth weight on the basis of data on genetic polymorphisms 10976 G/AFVII.
To assess the current patent situation was searched by the security documents for the period from 1990 to 2013, the Analysis of documents was made on a method of predicting birth weight on the basis of molecular genetic data depending on the polymorphic marker gene VII of the coagulation factor.
Known method for predicting body mass of the newborn according to the patent of Russian Federation №2472446, according to which determine the medico-biological factors, nutritional status, occupational risk and quality indicators�and lifestyle of parents in the formation of physical indicators of newborn. Birth weight is determined by a mathematical formula of the multiple regression equation: y=C+a1x1+a2x2+a3x3+...+anxnwhere xiinformative signs andithe coefficients for these signs, is a constant, namely:
Y1=2477,41-59,63 X1+Rate 82.46 X2+46,1 X3+0,88 X4- 0.44 X5+1,63 X6-5,005 X7-86,53 X8+345,93 X9+152,06 X10+1,56 X11Is -3.5 X12+493,13 X13+834,11 X14-127,71 X15+90,32 X16+397,68 X17-245,04 X18+418,51 X19-446,33 X20+174,90 X21,
where Y1- body weight of the newborn;
X1- the amount of protein in the diet of animal origin, X2- the amount of total protein in the diet, X3fats in the diet, X4sodium in the diet, X5- potassium in the diet, X6- calcium, X7- phosphorus; X8- iron, X9- Riboflavin, X10- Niacin, X11- vitamin C, X12- calorie diet, X13- total work experience of mother, X14- marital status; X15- a body mass index of women before pregnancy; X16- conditions of work of the mother; X17- stress in the workplace and in the family; X18- social conditions; X19- power mode; X20- experience of the child's father, X21- the nature of employment father.
A method according to�it possible to improve the accuracy of prediction of body weight of a newborn taking into account risk factors, influencing parents.
The disadvantage of this method is the consideration of a large number of significant risk factors, which complicates its use, as well as what is not taken into account such factors as the presence of genetic variants of polymorphism 10976 G/AFVII.
The objective of this study is to expand the Arsenal of methods for predicting birth weight, namely the creation of a method of predicting birth weight on the basis of data on the genetic polymorphism 10976 G/AFVII in combination with other predictors in women of Russian nationality, which urozhencami of the Central Chernozem region of Russia.
The technical result of the invention is the obtaining of the evaluation criteria predict birth weight in pregnant and in nonpregnant patients on pregravid stage in women of Russian nationality, which urozhencami of the Central Chernozem region of Russia.
In accordance with the assigned task has been developed a method of predicting birth weight at birth at term, 37 weeks or more of pregnancy, including determination of body weight of the newborn on a mathematical formula of the multiple regression equation: y=C+a1x1+a2x2+a3x3+...+anxnwhere xiinformative signs andithe coefficients for these traits, With the constant taking into account biological factors, which includes the following new features.
- extraction of DNA from peripheral venous blood;
analysis of polymorphisms of factor VII coagulation 10976G/A FVII;
- determining the weight of a newborn with repeated childbirth based on the following biological factors: the child's weight in the previous birth and growth of women and options identified by the locus 10976G/A FVII according to the equation:
y=6123,431-25,579 x1+0,267 x2+205,739 x3where y is the predicted birth weight, x1- the growth of women in centimetres; x2- the weight of the child in the previous birth in grams, x3genetic variant locus 10976G/A FVII, while for genotype 10976 GG FVII x3=1, for genotypes 10976 10976 GA and AA FVII x3=2.
- definition of birth weight in the first pregnancy given that biomedical factors as growth of women and options identified by the locus 10976G/A FVII according to the equation:
y=6278,037 - 21,739 x1+232,170 x2
where x1- the growth of women in centimetres; x2genetic variant locus 10976G/A FVII, while for genotype 10976 GG FVII x2=1, for genotypes 10976 10976 GA and AA FVII x2=2.
Novelty and inventive step lies in the fact that the prior art not known to the ability of forecast weight of the newborn for the presence of genetic polymorphic variants factoro� 10976 G/AFVII in combination with other predictors.
The method is as follows:
DNA extracted from samples of peripheral venous blood of pregnant in 2 stages. In the first stage to 4 ml of blood add 25 ml of lyse buffer containing 320 mm sucrose, 1% Triton X-100, 5 mm MgCl210 mm Tris-HCl (pH=7,6). The resulting mixture was stirred and centrifuged at 4 º C, 4000 rpm for 20 minutes. After centrifugation the supernatant decanted, to the residue add 4 ml of a solution containing 25 mm EDTA (pH=8.0) and 75 NaCl, resuspension. Then add 0.4 ml 10% SDS, 35 ál proteinase K (10 mg/ml) and incubated the sample at 37º for 16 hours.
In the second phase from the resulting lysate sequentially perform the extraction of DNA equal volumes of phenol, phenol-chloroform (1:1) and chloroform by centrifugation at 4000 rpm for 10 minutes. After each centrifugation produce a selection of the aqueous phase. DNA is precipitated from the solution with two volumes of chilled 96% ethanol. Formed DNA was dissolved in bidistilled deionized water and stored at -200S.
The selected DNA is then subjected to polymerase chain reaction using standard oligonucleotide primers.
Analysis of polymorphism 10976G/A (rs 6046) of the coagulation factor VII is carried out by polymerase chain reaction of DNA synthesis on the amplifier IQ5 (Bio-Rad) using a set of re�of gentou production OOO "Synthol"in accordance with the manufacturer's instructions.
Genotyping is carried out by the method of TagMan probes according to the values of relative fluorescence, then the UOF, each probe.
The invention is characterized in the figure, where the discrimination of alleles for the locus 10976 G/A of the coagulation factor VII, where ○ homozygotes 10976GG FVII, homozygotes 10976AA FVII, ∆ heterozygotes 10976GA FVII, ◊ - negative control. A probe with a fluorescent dye NEH corresponds to an allele And a probe with a FAM dye - allele G.
Two lanes, vertical and horizontal, divide the graph into four sections: one for each homozygous state, one for the heterozygous state and the section without reaction. The assignment of genotypes of unknown samples is determined by drawing a chart discrimination of alleles relative to the UOF for a single fluorophore on the x-axis, relative to the UOF for a different fluorophore on the y-axis.
• If the values of the UOF the unknown sample are above the horizontal stripes and the right vertical stripes, genotype heterozygote (GA).
• If the values of the UOF the unknown sample are above the horizontal stripes and vertical bars to the left, genotype homozygote for an allele a (UOF allele of A pending on the y-axis).
• If the values of the UOF an unknown sample are below the horizontal bars and to the right of vertical, genotype homozygote for an allele G (UOF G allele delayed by about�and x).
• If the values of the UOF an unknown sample are below the horizontal bars and to the left of vertical, the determination of the genotype is not possible, i.e. in this case the undefined sample - negative control.
The possibility of using the proposed method of predicting birth weight analysis confirms the results of observations of 454 patients, babies born at full-term pregnancy (37 weeks or more of gestation). In the current study included individuals of Russian nationality, which urozhencami of the Central Chernozem region of Russia and have no relationship among themselves. Among 454 pregnant women in 207 patients had placental insufficiency syndrome of delayed fetal growth, 247 women did not have this complication of pregnancy. These obstetric, somatic anamnesis and clinical and laboratory examination were recorded in a specially designed questionnaire. The following exclusion criteria were considered: the presence of uterine pathologies such as abnormal development of the internal genital organs, uterine fibroids; some pregnancy complications, such as anomalies of the location and attachment of the placenta, essensielle RH factor; fruit causes, including genetic diseases or congenital malformations, and the presence of multiple pregnancy. Kleene�technical and laboratory examination of pregnant women was conducted on the basis of the perinatal center of the Belgorod regional clinical hospital of Saint Joasaph. Diagnosis of the syndrome of intrauterine growth retardation of the fetus was performed on the basis of clinical data, namely the matching of the height of fundal gestational age, and were confirmed by the results of ultrasound fetometry and height and weight of the newborn. Placental insufficiency was confirmed by the results of histological examination of the placenta after birth. Molecular typing of genetic markers was carried out in the laboratory of human Molecular genetics" of the medical faculty of the Belgorod state national research University (supervisor - Professor, doctor of medical Sciences M. I. Churnosov). Creation of database and statistical calculations were performed using the program "STATISTICA 6.0".
A mathematical model for predicting birth weight was developed using multiple regression [Rebrov O. Y. Statistical analysis of medical data. The use of the STATISTICA software package [Text] /O. Y. Rebrova. - [3rd ed.]. - Moscow: Media Sphere, 2006. - 305 p.: ill.; Borovikov, V. P. Statistica: the art of computer data analysis [Text] /V. P. Borovikov. - 2nd ed. - St. Petersburg: Piter, 2003. - 688 p.].
When conducting multiple regression of the studied parameters revealed statistically significant (p<0.05) factors, anti-inflammatory.�ing influence on the formation of the weight of the newborn with repeated childbirth (table 1). Among them are: genetic polymorphism 10976G/A FVII, the weight of the child in the previous birth and growth of women. The determination coefficient obtained multiple regression models amounted to h2=0,16; F(3,178)=11,317;p<0,000001 (table 1). The error of this model is equal to 561,94 grams.
The multiple regression coefficients and level of significance of indicators used to predict birth weight
|Genetic variants of locus 10976 G/A FVII|
(1 - genotype 10976 GG FVII,
2 - genotypes 10976 10976 GA and AA FVII)
|Growth women, cm||- 25,579||0,001|
|The weight of the child in the previous birth, g||0,267||0,001|
It should be noted that the strength of the influence of the above variables on the weight of the newborn univariate models was at the level of 5-7% (h2=0,05; F(1,180)=10,157;p<holds 0.0000001% for the genetic polymorphism 10976G/A FVII; h2=0.07 respectively; F(1,180)=13,420; p<0,000001 for the growth of women; h2=0,05; F(1,180)=8,7523;p<holds 0.0000001% to the weight of the child in the previous birth. While taking into consideration their complex action the power of influence of these factors actually increases three times and reaches 16%.
Using the obtained coefficients of multiple regression on the basis of data on genetic variants of locus 10976G/A FVII, growth of the woman and weight of the child in the previous birth we can predict the weight of a newborn at birth at term, 37 weeks or more of pregnancy with repeated deliveries by the multiple regression equation of the following form:
y=6123,431-25,579 x1+0,267 x2+205,739 x3,
where y is the predicted birth weight, x1- the growth of women in centimetres; x2- the weight of the child in the previous birth in grams, x3genetic variant locus 10976G/A FVII, while for genotype 10976 GG FVII x3=1, for genotypes 10976 10976 GA and AA FVII x3=2.
To predict birth weight in nulliparous women was formed sample of 205 women who were pervorodyaschey at term gestational age of 37 weeks gestation or more. In the current study included individuals of Russian nationality, which urozhencami of the Central Chernozem region of Russia and have no relationship among themselves. Among them, 92 women had placental insufficiency syndrome of delayed fetal growth, and 113 - uncomplicated pregnancy. At the may�Institute multiple regression revealed statistically significant (p< 0.05) factors influencing the formation of birth weight in nulliparous. Among them are: genetic polymorphism 10976 G/A FVII and growth of the women (table 2). The determination coefficient obtained multiple regression models accounted h2=0,10;F(2,179)=10,142;p<0,00007. The error of this model is equal to 579,52 G. the impact of the above indicators on the weight of the newborn univariate models was at the level of 4-7% (h2=0,04; F(1,436)=18,732; p<0.0002 to genetic polymorphism 10976G/A FVII; h2=0.07 respectively; F(1,180)=13,420; p<0,0003 for the growth of women). While taking into consideration their complex action the power of influence of these factors increases to 10%.
Using the obtained coefficients of multiple regression on the basis of data on genetic variants of locus 10976G/A FVII and growth of women can predict the weight of a newborn at perforada at birth at term, 37 weeks or more of pregnancy.
The multiple regression coefficients and level of significance of indicators used to predict birth weight in nulliparous
|Genetic variants of locus 10976 G/A FVII|
(1 - genotype 10976 GG FVII,
2 - genotypes 10976 10976 GA and AA FVII)
|Growth women, cm||0,267||0,001|
Using the obtained coefficients of multiple regression, in the first pregnancy on the basis of data on genetic variants of locus 10976G/A FVII and growth of women can predict the weight of a newborn at birth at term, 37 weeks or more according to the multiple regression equation of the following form:
y=6278,037-21,739 x1+232,170 x2
where y is the predicted birth weight, x1- the growth of women in centimetres; x2genetic variant locus 10976G/A FVII, while for genotype 10976 GG x3=1 FVII, for genotypes 10976 10976 GA and AA FVII x3=2.
Examples of usage of the proposed method.
Example 1. Pregnant women C. according to molecular genetic examinations revealed genotype 10976GA FVII, i.e., x3=2, the growth of women amounted to 162 cm, weight of the newborn in previous births, 3100 grams. Substituting these values of attributes in a multiple regression equation
y=6123,431-25,579 x1+0,267 x2+205,739 x3and find y.
y=6123,431-25,579·162+0,267·3100+205,739·2=6123,431 - 4143,798+827,7+411,478=3218,811
Further monitoring of this patient showed that the weight of the newborn, who was born on �Roque 38-39 weeks amounted 3150 grams.
Example 2. Pregnant women D. according to molecular genetic examinations revealed genotype 10976GG FVII, i.e., x3=1, the growth of women was 170 cm, weight of the newborn in previous births, 3000 grams. Substituting these values of attributes in a multiple regression equation y=6123,431 - 25,579 x1+0,267 x2+205,739 x3and find y.
Further monitoring of this patient showed that the weight of the infant born at term 37-38 weeks, amounted to 2750 grams.
Example 3. Pregnant women F. (first birth) according to molecular genetic examinations revealed genotype 10976GG FVII, i.e., x2=1, the growth of women amounted to 160 cm Substituting these values of attributes in a multiple regression equation: y=6278,037 - 21,739 x1+232,170 x2and find y.
y=6278,037-21,739 x1+232,170 x2=6278,037-3478,24+232,170=3031,967
Further monitoring of this patient showed that the weight of the infant, born at 38 weeks, was 2710 grams. What is within the permissible error for this model.
Example 4. Pregnant women F. according to molecular genetic examinations revealed genotype 10976GA FVII i.e. x2=2, the growth of women amounted to 165 cm Substituting these values of attributes in a multiple regression equation y=6278,037-21,739 x1+232,170 x and find y.
Further monitoring of this patient showed that the weight of the infant born at term 40-41 weeks, was 3250 grams.
Thus, the proposed method allows a prediction of birth weight at term, 37 weeks or more of pregnancy in women of Russian nationality, which urozhencami of the Central Chernozem region of Russia, at first full-term pregnancy, depending on the genetic variants of coagulation factor 10976G/A FVII and given that biomedical factors as growth of women and prediction of birth weight in repeated deliveries depending on genetic variants of coagulation factor 10976G/A FVII with such biological factors as growth of women and birth weight in previous births.
Prognosis of babies with low birth weight will help to improve the complex of the actions directed on the prevention and treatment of this complication of pregnancy that will improve perinatal outcomes.
A method of predicting birth weight in women of Russian nationality, which urozhencami of the Central Chernozem region of Russia, taking into account biological factors in a mathematical formula of the multiple regression equation y=C+a1x1+a2x2+a3x
andithe coefficients for these characteristics;
C - constant;
characterized in that the DNA isolated from peripheral venous blood, analyze the polymorphisms of factor VII coagulation 10976G/A FVII, determine the body weight of the newborn at birth at term, 37 weeks or more of pregnancy, for women who give birth not for the first time, subject to such biological factors as growth of the woman and weight of the child in the previous birth, according to the equation:
y=6123,431-25,579 x1+0,267 x2+205,739 x3where
y is the predicted birth weight;
x1- the growth of women in centimetres;
x2- the weight of the child in the previous birth in grams;
x3genetic variant locus 10976G/A FVII, in this case, x3=1 for genotype 10976 GG FVII, x3=2 for genotypes 10976 10976 GA and AA FVII;
and for nulliparous women determine the body weight of the newborn at birth at term 37 + weeks pregnancy given as biomedical growth factor women by the equation:
y=6278,037-21,739 x1+232,170 x2where
x1- the growth of women in centimetres;
x2genetic variant locus 10976G/A FVII, x2=1 for genotype 10976 GG FVII, x2=2 for genotypes 10976 10976 GA and AA FVII.
SUBSTANCE: pre-operative fasting venous blood 1 ml is sampled at room temperature 20-24°C into an anticoagulant-free vacuum system (test tube). The test tubes are delivered in a sealed container at temperature 2-8°C for 2 hours to a laboratory for immunoenzyme assay and analysed to determine anti-thyroid stimulating hormone receptor antibodies. If the antibody level is 1.5 units/l or more, a thyroidectomy is performed, whereas the antibody level of less than 1.5 units/l requires performing a subtotal thyroid resection according to standard techniques.
EFFECT: invention enables reducing a probability of recurrent thyrotoxicosis in the patients after subtotal thyroid resection.
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of a risk of early microvascular complications in the children suffering from type 1 diabetes mellitus. The substance of the method consists in defining a duration of the diseases in years, the patient's age in years, a desquamated endothelial cell count, high-density lipoprotein cholesterol, total cholesterol, triglycerides, atherogenic index, glycohaemoglobin, average daily glycaemic level; making a linear regression analysis and calculating a risk ratio (R) of early microvascular complications in the children suffering from type 1 diabetes mellitus by formula. If the risk ratio is ≥1, the high risk of early microvascular complications during one year is predicted; the ratio < 1 shows the low risk of microvascular complications during one year.
EFFECT: using the declared method enables increasing the prediction accuracy of microvascular complications in the children with type 1 diabetes mellitus.
2 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine and can be used for patient's status monitoring. A method for setting a time-varying physiological parameter warning signal involves patient's controlled parameter monitoring, comparing the controlled parameter to an initial cut-off criterion, varying the cut-off criterion temporarily by a cut-off criterion of deterioration after the therapy, and then after a certain period of time, by the cut-off criterion after the administration. The time allowed involves comparing the controlled parameter to the cut-off criterion of deterioration, and after the time allowed - to the cut-off criterion after the administration. The warning signal is initiated in response to the controlled parameter of one or more initial cut-off criteria, the cut-off criterion of deterioration and the cut-off criterion after the administration. The group of inventions also refers to a machine-readable carrier with software for implementing the method and to a system for user warning on the controlled parameter variation.
EFFECT: group of inventions provides higher accuracy and rate of assessing the patient's status according to the conducted therapy.
15 cl, 6 dwg
SUBSTANCE: invention can be used for the purpose of the early prediction of cystic periventricular leukomalacia (PVL) in the newborns with very low (VLBW) or extremely low body weight (ELBW). Substance of the method: the newborns with VLBW and ELBW on the 3rd-7th day of life are examined to assess the perinatal medical history, namely the presence of chorioamnionitis and amniotic fluid nature, 5th minute Apgar score, the absence of prolonged artificial pulmonary ventilation, a severity of respiratory distress syndrome, the presence of pneumonia, sepsis, convulsive disorder, anaemia, laboratory signs of the systemic inflammatory reaction, average values of carbon dioxide, anionic bicarbonate and base deficiency in capillary blood, interleukine-6 and receptor interleukine-1 antagonist in venous blood serum. Each sign is assigned with a prognostic coefficient (PC). That is followed by determining total PC, and it is expected cystic PVL that is decided for if total PC is at least (+)9.5, whereas no cystic PVL is expected if total PC is (-)9.5 or less.
EFFECT: invention provides the early (for the first 7 days of life) prediction of cystic periventricular leukomalacia in the most susceptible category of patients that are children with very low and extremely low birth weight.
6 tbl, 5 ex
SUBSTANCE: interferon is measured in plasmocytoid dendrite cells at week 12 of the treatment. Its gain in adults and children twenty times as much as compared to the pre-treatment initial level, or 2.6×102 times as much in adults, and 1.2×102 as much as compared to the normal values, the stable positive antiviral treatment response is predicted.
EFFECT: method enables providing the more accurate prediction for selecting the optimum therapeutic approach in each patient.
SUBSTANCE: invention represents a method for assessing the mucosal immunity state of open cavities accompanying the prediction of the clinical course of infectious-inflammatory processes, characterised by the fact that having a pathogenetic factor established, degrees of microbiocoenosis disturbances of a specific biotope are recorded with the use of a complex of methods for estimating colonisation resistance factors, namely normal microflora, opportunistic microflora, immunoglobulins G, M, A, secretory immunoglobulin A and sc component; the mucosal immunity state is assessed according to the degree of microbiocoenosis disturbance, and the favourable outcome implying agent eradication or chronisation with agent persistence is predicted. The invention also refers to a method for correcting the infectious-inflammatory processes with the Kipferon® immunomodulator added.
EFFECT: increasing the prediction accuracy of the clinical course of the disease, extending the range of the method for correcting the infectious-inflammatory processes.
2 cl, 3 ex, 6 tbl, 1 dwg
SUBSTANCE: content of calcium and protein in oral liquid is determined before and after physical load, as well as a day after physical load. Recovery of content of calcium ions and protein in oral liquid after physical load to initial values is considered to be a criterion of total recovery of athlete's-volleyball player's organism, with evaluating time interval, required for said process.
EFFECT: invention makes it possible to determine reserve abilities of organism and its adaptability in athletes-volleyball players to physical load.
2 dwg, 4 tbl
SUBSTANCE: invention relates to the field of veterinary and can be used to diagnose the presence of a pathological effect of iron dextran on the piglets' liver. The essence of the method consists in carrying out the morphological systemic step-by-step analysis of histological liver cuts with the description of its histological structure, measurement of the average quantity of binucleate hepatocytes, apoptotic bodies and cells of the mononuclear-macrophage system. If the complex of pathognomonic morphological changes, including the presence of blood filling of sinus capillaries, oedema of the Disse's space; swelling of the vascular endothelium, granular dystrophy of hepatocytes, increase of the average quantity of cells of the mononuclear-macrophage system, including haemosiderophages by two times and more, as well as an increase of the quantity of binucleate hepatocytes and apoptotic bodies by 2 times and more, the effect of iron dextran on the piglets' liver condition is considered to be pathological.
EFFECT: invention provides an increased accuracy of the post-mortem diagnostics of the presence of a pathological effect of iron dextran on the piglets' liver.
SUBSTANCE: after thymomegalia has been excluded, tissue specimens of three-day-old mature newborns are studied to evaluate areas of inflammation changes in points in the placental umbilical cord (A), in the foetal placenta (B), in the maternal placenta (C), in extraplacental membranes (D); then thymomegalia is predicted by a discriminant equation: DE=-0.350×A-1.176×B-1.690×C-1.203×D, wherein DE is a discriminator function with a threshold equal to - 15.00. If DE is equal to or more than the threshold, the absence of thymomegalia is predicted; if D is less than the threshold, thymomegalia is predicted, whereas the score is taken at: (A) - 1 point - no inflammation, 2 points - amnionitis, 3 points - leukocytic infiltration in the Wharton's jelly, 4 points - phlebitis, 5 points - arteriitis, 6 points - a combination of two or more areas of inflammation: in blood vessels or in vessels and in the Wharton's jelly, (B) - 1 point - no inflammation, 2 points - chorioamnionitis, 3 points - villusitis, 4 points - vasculitis, 5 points - intervillesitis, 6 points - a combination of two or more areas of inflammation, (C) - 1 point - no inflammation, 2 points - villusitis, 3 points - vasculitis, 4 points - intervillesitis, 5 points - deciduitis, 6 points - a combination of two or more areas of inflammation, (D) - 1 point - no inflammation, 2 points - amnionitis, 3 points - chorioamnionitis, 4 points - deciduitis, 5 points - choriodeciduitis, 6 points - a combination of two or more areas of inflammation.
EFFECT: enabling the prediction of thymomegalia in the three-month-old mature newborns suffered prenatal influenza B complicated by placentitis.
FIELD: veterinary medicine.
SUBSTANCE: method consists in determining in the scales of concentration of Mn and/or Cu by the method of atomic-emission spectrometry. The regression equation is calculated, and on the content of Mn and/or Cu in the scales a copper concentration is determined.
EFFECT: method is accurate, atraumatic and non-invasive, simple and easy to use.
3 tbl, 1 ex
SUBSTANCE: invention refers to medical psychology, extreme medicine and aims at determining a risk of psychic deadaptation in internal affairs officers. The psychic deadaptation factors are estimated by the psychologic testing according to the multi-level personality questionnaire (MPQ) "Adaptability" and "Anxiety Study" technique by Ch.D.Spielberg and Yu.L Khanin, as well as officer's interview. The following parameters are specified in points: employment in the investigation division, communicative potential according to MPQ "Adaptability" technique, personal anxiety according to Spielberg-Khanin techniques, psychotic reactions and conditions according to MPQ "Adaptability" technique, smoking. The derived values are applied to an equation; and the produced value is used to determine the risk of psychic deadaptation: the high risk of psychic deadaptation or the low risk.
EFFECT: method enables determining the risk of psychic deadaptation in internal affairs officers by assessing the significant psychic deadaptation factors and interview.
FIELD: physics, computer engineering.
SUBSTANCE: group of inventions relates to ergatic systems and can be used to correct the functional status of a human operator. The method includes evaluating the status of a human operator. The status indicator used is the heart rate. Limiting values of the range of a good status ChSSmin and ChSSmax are set for each human operator. When the heart rate exceeds the limiting values, the energy stimuli used are light pulses and binaural action, the frequency f of which is set in accordance with a given expression. An apparatus for correcting the functional status of a human operator is used. The apparatus comprises a central control unit connected to a storage unit, a stimulus generating unit and an analysis unit. The stimulus generating unit is connected to a stimulation unit. The output of the analysis unit is connected to the central control unit. The apparatus additionally includes a unit for measuring and processing an electrical cardio signal and a heart rate determining unit. The output of the unit for measuring and processing an electrical cardio signal is connected to the input of the heart rate determining unit, the output of which is connected to the analysis unit.
EFFECT: group of inventions improves the efficiency of correcting the status of a human operator, prevents excessive fatigue of the human operator quickly and in advance, reduces emotional and psychological stress, raises the level of activity of the body owing to a method of determining the status of the human operator, using the heart rate ChSS as an indicator of the status, the effect of light stimuli and binaural action.
2 cl, 2 dwg, 1 tbl
SUBSTANCE: treatment is three-staged: preparation, restoration and confirmation. At the stage of preparation, pseudostuttering is differentiated from true stuttering, and pseudostuttering is relieved; protein delivery is ensured to body muscles by nutrition correction taking into account the patient's blood type. Desynchronosis is eliminated by synchronising the patient's biological rhythms by matching pulse beats per minute and vocal exhalation rhythmicity accompanied by executing fist motions by clenching and opening it as a multiple of a given rhythm frequency, and a walking rhythm. Speech exercises aiming at developing speech fluidity, thinking and motor coordination exercises are performed. The stage of restoration involves eliminating the causes of true stuttering by activating respiratory, laryngeal and articulation muscles: stuttering is eliminated by localising respiratory spasms by the abdominals. Stuttering is eliminated where laryngeal spasms are localised, by adding laryngeal exercises to the above combined exercises, including internal laryngeal muscular contractions: sialophagia, singing, speaking, reading aloud; stuttering is further eliminated where articulation spasms are localised by adding speech apparatus muscle exercises to the above combined exercises and giving face massage, executing mimic exercises aiming at oro- and nasopharyngeal muscles exercises. At the stage of confirmation, the developed speech skills are confirmed until natural speech skill is developed, by keeping on doing restoration practices. The method enables the recurrence-free elimination of the causes of stuttering at any age and any intensity of stuttering with tonic or clonic convulsions of the vocal apparatus muscles, notwithstanding both neurotic, and neurosis-like types by applying correction and compensation non-drug therapy.
EFFECT: treating stuttering both under the guidance of a specialist, and independently.
SUBSTANCE: analgesics timing and amount are determined according to peak points of pain syndrome manifestations, which are detected by assessing pain syndrome intensity within 24 hours according to the scale with 12 time intervals. The patient is supposed to fill in the time interval form: 1-3; 3-5; 5-7; 7-9; 9-11; 11-13; 13-15; 15-17; 17-19; 19-21; 21-23; 23-1 and records pain intensity every two hours. Each time interval matches with a modified visual analogue scale, which combines elements of verbal expression of pain and is divided into four sub-scales to assess pain as follows: mild, moderate, pronounced and severe; the origin of the scale corresponds to the pain-free state, whereas the end of the scale stands for unsufferable pain.
EFFECT: method enables correcting the analgesics and anti-pain electric stimulation timing that is expected to provide the higher efficacy of the antalgic therapy by having the pain syndrome intensity determined within 24 hours in accordance with the 12-interval scale.
7 dwg, 1 ex
SUBSTANCE: method applies a visual system of depth-effect 2D image perception; ocular movements are recorded by a binocular eye-tracker; right and left eye lines are sensed. At the first stage: Io image is selected, wherein the person being tested perceives image relief and depth effects. The Io is displayed on a monitor screen of the binocular eye-tracker, spaced from the eyes at 0H; a right (Ra) and left (Le) eye line X-coordinate array is recorded during the period ΔT of measurement. A coordinate difference is calculated by formula ΔX=LeX-RaX; a coordinate difference histogram is contoured; a difference histogram contour peak is localised as a peak density of eye focusing planes, a contour line range, and focusing planes. A distance maxH to the difference histogram contour peak is calculated on the left lH and right rH contour lines; provided maxH≠0H, then the differences ΔH=lH-rH are derived, and objective physiological makers of the relief effect that are the parameters independent of an estimation offered by the person being tested and recorded by the binocular eye-tracker are detected. They are compared to a relief analogue that is a 3D-pixel image perception depth 3DIo. A coordinate difference 3DΔX is calculated; a difference histogram is contoured; a difference histogram contour peak is localised as a peak density of eye focusing planes, a contour line; a distance 3DmaxH to the contour peak and the eye focusing plane, and the contour line is measured. Provided the distances 3DmaxH, 3DΔH fall within the distance range ΔH=lH-rH≠0, with maxH≠0H, general physiological principles of the relief effect perception and the depth perception of the pixel images between 3DIo and Io are stated. They are referred to reference plane images IR, which describe positions of the right and left eye focusing planes within the distance range ΔH=lH-rH≠0, with maxH≠0H. The second stage involves drawing independent samples of the people being tested who are presented with the reference plane images IR formed at the first stage. Relief build-up values are derived on the images IR, statistical relief tracking diagrams on the analysed images are constructed. The relief perception for independent samples of the people being tested is matched, and the general principles of the plane image relief perception are laid down.
EFFECT: method enables identifying the principles of unconscious perception of the image relief perception by recording the eye motions by the binocular eye-tracker.
5 dwg, 1 ex
SUBSTANCE: invention refers to medicine, particularly to psychology, and can be used for individual and mass screening in sociology and management. A person being tested is presented with a number of pictures to be selected. The pictures are composed so that each picture contains an image that combines two archetypes with adequate shape and background. The person being tested is suggested to select among the presented number of pictures the most relevant of his/her personality evaluation and to sample. The person being tested is suggested to assign a score to the selected picture. The numerical treatment of the results involves summing up the assigned scores of the same archetypes. The number of pictures can make 20 with each picture containing two combined images with the total number of archetypes equal to 10. The total score is distributed among 1 to 8, whereas the maximum diagnostic number is equal to 10, which is when exceeded enables stating an archetypic identity.
EFFECT: method enables assessing the archetypic identity, the presence of neurotic pattern and intrapersonal conflicts by analysing the archetype-based psychological personality profile.
5 cl, 2 dwg, 1 tbl
SUBSTANCE: method involves finding anamnestic data, clinical signs, work experience in allergically harmful production factors, elimination symptom, symptoms of the disease immediately during employment and deterioration after return to the harmful conditions, total blood serum IgE concentration. Each parameter is appraised by points. The points are summed up, and the derived total score is used to predict a low risk of occupational allergodermatosis, a moderate risk or a high risk.
EFFECT: method enables more accurate prediction ensured by determining significant parameters.
3 tbl, 3 ex
SUBSTANCE: invention refers to medicine, namely to animal's behavioural physiology. The orientational-exploratory and locomotor behaviour in rats is rated with an underlying developing acquisition of a food-producing skill by differentiating an animal's motion path in a batwing-shaped labyrinth. The rating procedure is based on the following forms of motor actions: movement to the labyrinth loci without a food reward; purposive movement to the locus with the food reward; exploratory travelling through all the labyrinth landings; movements to the locus contra lateral with the food reward; multiple movements between the two labyrinth loci; freezing behaviour in the start position.
EFFECT: method enables increasing the investigation reliability that is ensured by a multiple assessment of the central nervous system functions.
2 cl, 1 tbl, 1 ex, 6 dwg
SUBSTANCE: psychoemotional state in children and adolescents is assessed by a projective test of fairy-tale characters by presenting pictures of the fairy-tale characters to the persons being tested. The presented pictures contain a number of images of the fairy-tale characters grouped according to their archetypes. The person being tested is suggested to choose the first mini set of the characters having the most subjectively positive characteristics, as well as to choose the second mini set of the characters with the most subjectively negative characteristics from the rest. The number of the characters of the both mini sets is less than the total number of the characters. The characters chosen from both sets first are assigned with the highest point; the characters chosen next are assigned with descending points according to the degree of priority. A procedure of mathematical processing represents summing the points assigned to the characters of the same archetype, and the derived total point of the characters of the same archetype is used to conclude the personality.
EFFECT: method enables increasing the effectiveness and objectivity of the integral assessment of the personal psychological profile in the adolescents and children by using the projective method.
5 cl, 4 dwg, 10 tbl, 1 ex
SUBSTANCE: individuals with different known psychic types are filtered. Examined individual's and filtered individual's external parameters are selected and measured. The examined individual's selected and measured external parameters are compared to the same filtered individual's external parameters, and the examined individual's psychics is referred to the filtered individual's psychic type having the maximum similarity of the external parameters. The filtration procedure can also cover the individuals with various known psychics typed on the basis of the characteristics of ethics-logic, intuition-sensory, extroversion-introversion, rationality-irrationality or a type of temperament.
EFFECT: method enables providing the higher reliability of the obtained results when typing the individual's psychics by determining the significant similarity of the individual's external parameters to the close individual's psychic structure.
4 cl, 2 tbl
SUBSTANCE: method involves forming signals as tests requiring solution. The tests are shown with frequency changed proportionally to the frequency they are solved. The number of tests is set to be the same in the cases of recovered and tired state. General amount of time spent for finding solution for given number of tests and the number of tests having right solutions are determined in each state. Mental fatigue degree is evaluated from relative change of mean time needed for finding the right test solution using a relationship like (Tm.r- Tm.t)100%/Tm.t, where Tm.t = Tsum.t/Kr.t, Tm.r = Tsum.r/Kr.r, Tm.t is the time spent for finding the right answer in tired state, Tm.r is the time spent for finding the right answer in recovery state, Tsum.t is the total time spent to solve given number of tests in tired state, Tsum.r is the total time spent to solve given number of tests in recovered state, Kr.t is the number of right answers to the tests in tired state, Kr.r is the number of right answers to the tests in recovered state.
EFFECT: reliable estimation of fatigue degree.