Method for laboratory control of physical load level on athlete's-volleyball player's organism

FIELD: medicine.

SUBSTANCE: content of calcium and protein in oral liquid is determined before and after physical load, as well as a day after physical load. Recovery of content of calcium ions and protein in oral liquid after physical load to initial values is considered to be a criterion of total recovery of athlete's-volleyball player's organism, with evaluating time interval, required for said process.

EFFECT: invention makes it possible to determine reserve abilities of organism and its adaptability in athletes-volleyball players to physical load.

2 dwg, 4 tbl

 

The invention relates to the field of sports medicine, to methods of laboratory diagnostics of the level of physical load on the athlete's body.

In modern professional sports is required daily (monitoring) assessment of the adequacy of the proposed athletes training loads them dynamically changing level of operational readiness training complex and competitive activity. Efficient allocation of rehabilitation measures at different levels of the structure of the training process largely determines the improvement of physical fitness of athletes and the achievement of high and stable results. Assessment of the level of fitness of the athlete should be comprehensive, therefore it is important to find simple affordable methods of Express-diagnostics of a condition of an athlete before and after exercise for the purpose of conducting training and rehabilitation activities.

Currently, a diagnosis of "overtraining syndrome" remains one of the challenges of sports medicine. Systematic monitoring of the current state of health of athletes through the standard sports-medical tests, and by far the most sensitive method to detect the condition p�retrovirologist.

Current methods of functional and diagnostic testing of athletes to a certain extent complex, time consuming and not sufficiently informative, require specially trained, experienced medical personnel. For periodic current functional control over the level of readiness of the athlete to training and competition loads required search simple, informative, accessible methods of Express-diagnostics, for example based on the analysis of changes in the physico-chemical parameters of saliva.

Use for diagnostic purposes it is saliva has several advantages compared to existing methods of diagnosis. Since the collection of samples of saliva may be produced as in the process of training and rest periods, without the participation of specialized medical personnel. There is an opportunity to replace weekly or monthly profiles on a per diem. Moreover, samples of saliva a long time can be stored in the refrigerator; the ease of a multiple sampling; low cost; ease of studying in normal physiological conditions.

Currently known methods of assessing the level of physical capacity of a person by how much work was done to a drastic decrease of threshold values marempolskoho interval (application No. 2008118077/14, 06.05.2008) and Reza�Tatum 12-minute step test (application No. 2005137626, 02.12.2005); method for determining the physical fitness of the individual in mass surveys, tests on General and strength endurance, flexibility, reaction time and coordination (application No. 2006143680/14, 01.12.2006); method of complex functional diagnostics in forced expiratory volume in unit time, the delay time of breathing, the partial pressure of carbon dioxide of blood, etc. (application No. 96111049, 04.06.1996)4; the method of assessing the functional status of hockey players using the rapid analysis of urine (application No. 2002109509/14, 11.04.2002).

These methods are uninformative, rather lengthy, require specially trained personnel to conduct surveys and not suitable for mass functional and diagnostic research.

The closest in technical essence to the claimed method is a laboratory method of monitoring the level of physical load on the body of athlete, badminton player (application No. 2463962(376), publ. 20.10.2012). In this method, control of physical condition based on the determination of calcium and phosphorus in saliva before and after exercise. This method has significant limitations, as it only applies to athletes, badminton players that are different from other sports exercise regime.

The objective of the claimed invention is non-invasive�about laboratory method of monitoring the level of physical load on the body of the athlete-volleyball player to identify the level of adaptation and the reserve capacity of the organism, applicable for large-scale studies in terms of the ambiguity of the criteria of norm and pathology.

Said technical result is achieved in that in the method of laboratory testing of physical load on the body of the athlete - volleyball player determine the content of calcium and protein in the saliva before exercise, after exercise, day after exercise and recovery parameters of oral fluid to the initial values considered the criterion of full recovery of an athlete-volleyball player, estimating the time needed for this process.

The method is carried out as follows. To determine the parameters of oral fluid following studies were conducted. Material study served as saliva 78 athletes aged 18 to 22 years. Saliva was collected in the same conditions, no earlier than 2 hours after eating and rinsing the mouth with tap and distilled water in a sterile tube with tight fitting lid. Saliva collection was performed at rest and after exercise. The collected saliva was centrifuged for 10 minutes at a speed of 3000 rpm in a laboratory medical centrifuge APL-8. For the determination of physicochemical parameters of oral fluid has been used techniques, a more tailored�ƈ in dentistry for the study of the oral liquid [Petaev A. N., Korshunov A. P., V. A. Distel et al. "Physico-chemical methods in mixed saliva in clinical and experimental medicine" (manual) - Omsk, 2001]. Inorganic phosphorus in the oral fluid was determined by the method Bolza and Luke [Bolz D. M., Luke C. G. "Colorimetric methods for determination of metals", Wiley, 1963], for the quantitative determination of protein used biuret reaction [Leus P. A. "Clinical and experimental study of the pathogenesis, pathogenic conservative therapy and prevention of dental caries". The author's abstract dis. Dr. honey. Sciences. - M., 1977]. Mathematical data processing was performed using the statistical package STATISTICA 6.0 (Stat Soft Inc. USA).

In parallel, we performed functional diagnostic examination of athletes, this takes into account the following parameters:

- General personal data (gender, age, height, weight, BMI);

- preparedness of the athlete (the skill level of the athlete, the view of the nature and type of physical activity, the specificity and difference of activity within the group, consideration of the level of stress and bioenergetic costs).

Experiment with volleyball was repeated twice with an interval of 1 week. Athletes on two different received the same training physical exercise: interval training, mixed aerobic and anaerobic; duration Tr�of miravci 90 minutes.

Previously, the experimental data were processed using multivariate statistical methods (discriminant and cluster analyses, Statistica 6.0, StatSoft).

Fig. 1 shows a scatterplot of the canonical values of volleyball players (1 training) before, after and beyond (2). Fig. 2 shows a scatterplot of the canonical values of volleyball players (both workouts) before, after, and beyond. In both cases there is significant difference of parameters of oral fluid athletes volleyball players of all studied groups before and after exercise, with options to load and after recovery are almost the same.

It is shown that after exercise (see tab.1-4) there is a material change in the composition of saliva, in particular, reduced the concentration of calcium ions, while increasing protein content. Thus there is a decrease in the ratio of CA/P, which characterizes the equilibrium of the processes of mineralization and demineralization in the human oral cavity, which indicates the physiological disturbances of equilibrium, due to high physical stress on the body.

Because in the conditions of the training process criteria and norms � pathology are ambiguous, additionally, we calculated the rate of change of investigated parameters of oral fluid. As can be seen from the above data (see tab. 4), the most convenient, in our opinion, is to assess the degree of change of parameters of oral fluid content of calcium and protein in the saliva before and after exercise. The value of the degree of change in these parameters for the study groups 56.5% and 45.7%, respectively.

It should also be noted that the daily rest between workouts leads to the restoration of parameters of oral fluid athletes to the original values, this suggests the possibility of carrying out of training after post-exercise recovery and improve the adaptive abilities of an athlete.

Thus, the deviation of parameters of oral fluid from the initial values can be used for laboratory monitoring of the level of physical activity for athletes-volleyball players. The return parameters of oral fluid to the initial values considered the criterion of full recovery in athletes-volleyball, estimating the time needed for this process.

Thus, the claimed method allows to carry out laboratory diagnosis of the level of physical activity on the human body for the purpose of identification UB�nya adaptability and reserve opportunities of an organism, applicable for large-scale studies in terms of the ambiguity of the criteria of norm and pathology.

Method of laboratory diagnostics of the level of physical load on the body of the athlete-volleyball player, namely, that determine the content of calcium and protein in oral fluid before exercising, after exercise and after exercise, recovery of the content of calcium ions and protein in oral fluid after exercise to their original values considered the criterion of full recovery of an athlete-volleyball player, estimating the time needed for this process.



 

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1 tbl, 8 ex

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4 ex

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2 dwg, 2 ex

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1 dwg, 1 tbl, 3 ex

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3 ex

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