Method for prediction of thymomegalia in three-month-old mature newborns sufferedprenatal influenza b complicated by placentitis
SUBSTANCE: after thymomegalia has been excluded, tissue specimens of three-day-old mature newborns are studied to evaluate areas of inflammation changes in points in the placental umbilical cord (A), in the foetal placenta (B), in the maternal placenta (C), in extraplacental membranes (D); then thymomegalia is predicted by a discriminant equation: DE=-0.350×A-1.176×B-1.690×C-1.203×D, wherein DE is a discriminator function with a threshold equal to - 15.00. If DE is equal to or more than the threshold, the absence of thymomegalia is predicted; if D is less than the threshold, thymomegalia is predicted, whereas the score is taken at: (A) - 1 point - no inflammation, 2 points - amnionitis, 3 points - leukocytic infiltration in the Wharton's jelly, 4 points - phlebitis, 5 points - arteriitis, 6 points - a combination of two or more areas of inflammation: in blood vessels or in vessels and in the Wharton's jelly, (B) - 1 point - no inflammation, 2 points - chorioamnionitis, 3 points - villusitis, 4 points - vasculitis, 5 points - intervillesitis, 6 points - a combination of two or more areas of inflammation, (C) - 1 point - no inflammation, 2 points - villusitis, 3 points - vasculitis, 4 points - intervillesitis, 5 points - deciduitis, 6 points - a combination of two or more areas of inflammation, (D) - 1 point - no inflammation, 2 points - amnionitis, 3 points - chorioamnionitis, 4 points - deciduitis, 5 points - choriodeciduitis, 6 points - a combination of two or more areas of inflammation.
EFFECT: enabling the prediction of thymomegalia in the three-month-old mature newborns suffered prenatal influenza B complicated by placentitis.
The invention relates to medicine, namely to Perinatology and Pediatrics.
It is known that in women with acute respiratory viral infections in pregnancy complicated by placental insufficiency [2, 3, 4], it is noted in utero stimulation of the immune system, leading to the development of thymomegalia their offspring . However, the size and weight of the thymus, antenatal contact with respiratory viruses, does not always change in children at birth and can grow only during the first months of life in the process of their adaptation to various antigens  and prolonged stimulation of the adrenal cortex . It is established that the increase in the number of foci of inflammation in the placenta increases the risk of infectious diseases in neonates , and in the placenta (deciduate) increases the risk of hyperplasia of the thymus gland in fetuses .
The known method of qualitative morphological assessment of inflammation in the placenta  does not allow to predict the timomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta.
The objective of the proposed method is the ability to predict thymomegalia within three months of age in full-term neonates with intrauterine influenza, complicated by placenta.
The objective of dostigao�Xia, the prediction of thymomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta, is carried out by determining the number of foci of inflammatory changes in the umbilical cord (in points) (A), the number of foci of inflammatory changes in the fetal part of the placenta (in points) (B), the number of foci of inflammatory changes in the maternal part of the placenta (in points) (C), the number of foci of inflammatory changes in unpleasantry shells (in points) (E), and then carry out a forecast of thymomegalia using discriminant equation:
D - discriminant function with boundary value is 15.00.
When D is equal to or greater than the boundary values, predict the absence of thymomegalia, when D is less than the boundary values predict timomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta.
The method is as follows.
1. In full-term neonates with intrauterine influenza diagnosed by serologic analysis of response inhibition of haemagglutination) serum in pairs "mother-child", as well as by immunofluorescence studies of brushstrokes-prints of the nasal mucosa or secretions of the genome of the pathogen with the help of� polymerase chain reaction , performed ultrasound of the thymus at 3 days of life to avoid thymomegalia.
2. In full-term neonates with intrauterine influenza, histological preparations of placental side of the umbilical cord hematoxylin-the Böhmer - eosin, determine the number of foci of inflammatory changes in points (1 point - no inflammation; 2 points - amnionic; 3 points - leukocytic infiltration in Vartanova jelly; 4 points - phlebitis; 5 points - arteritis; 6 points - combination of 2 or more foci of inflammation in blood vessels or in the vessels and in Vartanova jelly) (A).
3. On histological preparations of the Central portion of fruit part of the placenta hematoxylin-the Böhmer-eosin, count the number of inflammatory foci in points (1 point - no inflammation; 2 points - chorioamnionitis; 3 points - villosa; 4 points - vasculitis; 5 points - intervilles; 6 points - combination of 2 or more foci of inflammation) (B).
4. On histological preparations of the Central portion of the maternal part of the placenta hematoxylin-the Böhmer-eosin, set the number of inflammatory foci in points (1 point - no inflammation; 2 points - villosa; 3 points - vasculitis; 4 points - intervilles; 5 points - decidua; 6 points - combination of 2 or more foci of inflammation) (C).
5. On histological preparations unpleasantry� membranes (the fracture), hematoxylin-the Böhmer-eosin, count the number of inflammatory foci in points (1 point - no inflammation; 2 points - amnionic, 3 points - chorioamnionitis; 4 points - decidua; 5 points - choriodecidua; 6 points - combination of 2 or more foci of inflammation in unpleasantry shells) (E).
6. Using discriminant equations that determine the magnitude of discriminant functions:
D - discriminant function with boundary value is 15.00.
7. Compare the value of the discriminant function from its boundary value is 15.00.
8. Predict the development of thymomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta: when D is equal to or greater than the boundary values, predict the absence of thymomegalia, when D is less than the boundary values predict timomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta. The probability of a correct prediction was 89.1%.
To illustrate the effectiveness of the proposed method of predicting thymomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta, the following clinical examples.
Newborn C. mother's first pregnancy, p�regular was observed in the antenatal clinic with 7 weeks. At 16 weeks of gestation showed signs of flu confirmed in the study of paired sera by reaction of hemagglutination-inhibition (antibody titer of 1:4-1:16). Flu proceeded with the clinic rhinopharyngitis, fever up to 38°, headache, cough, runny nose and weakness. At 28 weeks, was diagnosed placental insufficiency, about which the mother was treated in the obstetric hospital. Blood group A (II), RH - positive. First birth at term. Amniotic fluid light.
Clinical diagnosis of the mother: a Birth in the first term, quick. Complicated current of pregnancy (flu). Chronic placental insufficiency. Amniotomy.
The boy was born with the weight of 3350 g, length 53 cm, head circumference - 35 cm chest - 34 see blood Group b (III) RH - positive. In the blood from the vein of the umbilical cord of the child's total hemoglobin - 215 g/l, leucocytes - 28×109/l, eosinophils - 1%, stab neutrophils - 6%, segment reflexology point pressure-nuclear neutrophils - 70%, lymphocytes - 20%, and monocytes - 3%. The concentration of antibodies to influenza virus B in serum pairs "mother - newborn" - 1:32-1:128.
A newborn's condition at birth was close to satisfactory. Was observed cyanosis of nasolabial triangle, decreased activity, muscle tone and tendon reflexes. Physiological reflexes were expressed as odd�O. Heart tones are clear, rhythmic to 144 beats per 1 minute. Respiratory rate - 46 in 1 minute. The breath in the lungs puerile. The external genitals are developed correctly by male type. Peeing in the delivery room. When the pathological examination of the placenta in the umbilical cord was noted foci of leukocytic infiltration of the amnion (2 points), in the fetal part of the placenta showed morphological signs of chorioamnionitis (2 points), in the maternal part of the placenta - vasculitis (3 points), in unpleasantry shells - amnionitis (2 points). With ultrasound the baby was not observed increase of the thymus at 3 days after birth compared with its size and weight in healthy full-term newborns .
Clinical diagnosis of the newborn: Intrauterine infection (flu - influenza titers of antibodies in the system "mother - newborn" 1:16-1:64).
The above indicators were then entered into a discriminant equation:
D=-0,350×2-1,176×2-1,690×3-1,203×2=-10,53, where D is the discriminant function with boundary value is 15.00.
Predicted lack of development of thymomegalia 3 months of life in term newborns with intrauterine influenza and In the placenta.
When holding the child sonographic studies have not been diagnosed timomegalia within three months of age.
Newborn P. was Born from the first pregnancy. At 20 weeks the mother suffered from influenza In (antibody titer of 1:16-1:64) with a temperature rise up to 37,5°, with a headache, cough, runny nose and weakness. At 29 weeks was diagnosed placental insufficiency, for which she was treated in the hospital. Blood group B (III), RH - positive. In female consultations were observed regularly from 7 weeks of pregnancy. Birth vaginally. Amniotic fluid light. Clinical diagnosis of the mother: a Birth in the first period. Premature discharge of amniotic fluid. Chronic placental insufficiency.
The boy was born weighing 3460 g, length 53 cm, head circumference - 34 cm and chest - 33 cm blood type A (II), RH - positive. In the venous blood from the umbilical cord of the child at birth, total hemoglobin was 210 g/l, white blood cell count of 14.2×109/l, eosinophils - 2%, stab neutrophils - 4%, segmented neutrophils 72%, lymphocytes - 20%, and monocytes - 2%. The concentration of antibodies to influenza virus In the sera of pairs "mother - newborn" was 1:16-1:64.
A newborn's condition is satisfactory. The skin of the face, extremities and trunk was clean. Was observed cyanosis of nasolabial triangle, as well as reduced muscle tone and tendon reflexes. Determined all physiological reflexes. Tones with�rdca - clear, rhythmical to 142 beats per 1 minute. Respiratory rate 48 in 1 minute. The breath in the lungs puerile. External genitalia were well developed correctly by male type.
When pathological examination of the placenta in the umbilical cord of the child was determined leukocytes in Vartanova jelly (3 points), in the fetal part of the placenta was diagnosed with intervilles (5 points) in the maternal part of the placenta - villosa (2 points), in unpleasantry shells - a combination of deciduata and chorioamnionitis (6 points). Sonographically not detected an increase organometric indicators of the thymus at 3 days after birth compared with its parameters in healthy term newborns.
Clinical diagnosis of the newborn: Intrauterine infection (influenza b, influenza titers of antibodies in the system "mother - newborn" 1:64-1:256).
The above symptoms were entered into the discriminant equation:
D=-0,350×3-1,176×5-1,690×2-1,203×6=-17,53, where D is the discriminant function with boundary value is 15.00.
Dynamic observation of the child in a children's polyclinic at 3 months had been diagnosed with timomegalia.
The method was tested in the survey 44 full-term newborns with antenatal influenza and placenta.
A correct prediction was confirmed at 89.1% of cases.
The technical result of the use of�izlagaemogo method of prediction is a method for predicting thymomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta, will allow for timely therapeutic measures aimed at desensitization, detoxification and improving children's immune resistance of the organism to respiratory viral infections in early neonatal age. Thus, the role of timely identify pathological foci of inflammation in born the placenta and their numerical score, allowing the use of discriminant analysis to establish the probability of development in the three month old thymomegalia that in pediatric practice is usually associated with adrenal insufficiency , with impaired pituitary-adrenal relationships and dysfunction of T-cell immunity  and with decreased resistance to viral infections .
Sources of information
1. Grigorenko A. A. Morphological and histochemical changes of the endocrine glands in sepsis in children: author. dis. Cand. honey. of Sciences, Novosibirsk, 1981. - 19 p.
2. Gorik I. N., Voskoboynikov, O. R., Mirlas M. F., Yarovoy A. A., the uterine-placental, placental and fetal blood flow in the third trimester of pregnancy complicated with acute respiratory viral infections, pyelonephritis and OPG-gestoso� // far East medical. log. - 1997. - No. 2. - P. 51-52.
3. Gorik I. N. The pathogenesis of the changes of blood flow in uterine arteries and ultrasound picture of the chorion in influenza A(H3N2) in women in the first trimester of pregnancy // bull. physiology. and patola. breathing. - 2012. - Vol.44. - P. 79-84.
4. Gorik I. N. The change in uterine blood flow and ultrasonic patterns of fetoplacental complex in influenza A(H3N2) in women in the second trimester of pregnancy // bull. physiology. and patola. breathing. - 2012. - Vol.45. - P. 74-78.
5. T. V. Zabolotskikh, Grigorenko A. A., Gorik I. N. The change in ultrasonic and morphological structure of the thymus in newborns with fetal parainfluenza types 1 & 3 // bull. physiology. and patola. breathing. - 2013. - Vol.50. - Pp. 94-98.
6. Netreba N. And. Features of immune responses in young children and the role of maternal immunity in protection against acute respiratory viral infections // Antenatal care in pregnancy and prevention of perinatal pathology / Theses of reports, the Ministry of health of the Ukrainian SSR, Kiev scientific research Institute of Pediatrics, obstetrics and gynecology named after Hero of the Soviet Union, Professor P. M. buyko. Kiev, 1979. Pp. 190-191.
7. A method of predicting risk of development of intrauterine infection in newborns: us Pat. 2168946 of the Russian Federation, IPC A61M 1/00 / M. P. Openheim, V. A. Medik, V. N. Soloviev, P. E. Murmulev, A. R. Nosov; applicant and patentee: closed joint stock company SOMA. - No. 2000112897/14; s�. 24.05.2000; publ. 20.06.2001.
8. Zinserling A.V., Glukhovets N. G. The broadening of the pathoanatomical studies of fetuses and placentas for late spontaneous abortions // Ross. Bulletin of the women. and Pediatrics. - 1994. - Vol. 39, No. 2. - P. 8-10.
A method of predicting thymomegalia within three months of age in full-term neonates with intrauterine influenza complicated by placenta, which consists in the fact that in full-term infants after exclusion of thymomegalia 3 days of life is determined on histological preparations the number of foci of inflammatory changes in scores in the placental side of the umbilical cord (A), in the fetal part of the placenta (B), in the maternal part of the placenta (C), in unpleasantry membranes (E), and then carry out a forecast of thymomegalia using discriminant of the equation:
where D is the discriminant function with boundary value is 15.00, moreover, if D is equal to or greater than the boundary values, predict the absence of thymomegalia, when D is less than the boundary values predict timomegalia, and the number of points is the rate of: (A) - 1 point - no inflammation, 2 points - amnionic, 3 points - leukocytic infiltration in Vartanova jelly, 4 points - phlebitis, 5 points - arteritis, 6 points is a combination of two or more foci of inflammation in blood vessels or in the vessels and in Vartanova �tune, (In) - 1 point - no inflammation, 2 points - chorioamnionitis, 3 points - villosa, 4 points - vasculitis, 5 points - intervilles, 6 points is a combination of two or more foci of inflammation, (C) - 1 point - no inflammation, 2 points - villosa, 3 points - vasculitis, 4 points - intervilles, 5 points - decidua, 6 points is a combination of two or more foci of inflammation, (E) - 1 point - no inflammation, 2 points - amnionic, 3 points - chorioamnionitis, 4 points - decidua, 5 points of choriodecidua, 6 points is a combination of two or more foci of inflammation.
SUBSTANCE: after the ablation of the spleen, its weight is determined, the average area of a marginal zone of the spleen in histological cuts with coloration with hematoxylin and eosin is measured morphometrically. The obtained values are used to calculate the weight of the marginal zone and, if its values are ≤1.9 g, a favourable prognosis for the course of aplastic anaemia is made, if its value is >1.9 g, an unfavourable course of the disease is predicted.
EFFECT: prognosis of the aplastic anaemia course after splenectomy independent on the severity of the disease with the possibility to approach therapeutic treatment in a differential way.
SUBSTANCE: gastric content pH is dynamically estimated with the use of a nasogastric tube; the total gastric content pH is measured and the efficacy of the antisecretory preparations is estimated for one day, or a longer period of time if needed, every 3 hours. The pH value is measured by means of an analogue display unit on an analogue scale at a pitch of 1.0 within 1.0 to 12.0 for 15 s as shown by discolouration, with matching to the analogue scale. The acid production suppression is considered to be effective at pH of more than 4.0 after planned surgeries, and more than 6.0 after emergency surgeries. If the acid production suppression is found to be ineffective, the antisecretory preparation is supposed to be changed or increased in dose.
EFFECT: method enables measuring the gastric content pH and estimating the efficacy of the antisecretory preparations in the patients in need thereof over a short period of time.
2 dwg, 2 tbl, 3 ex
SUBSTANCE: method of neurosyphilis diagnostics includes the microscopic analysis of cerebrospinal fluid samples, with carrying out edge dehydration of the cerebrospinal fluid samples, and their microscopic analysis being carried out in a polarised light; in case of the detection of anisotropic structures in the form of dendrites or spherulites inside which oval-shaped formations, containing lipids, are located, an early form of neurosyphilis is diagnosed; and in case of the detection of a multitude of ovals, aggregated in the form of balls, included in the anisotropic structures and/or located separately, late meningovascular neurosyphilis is diagnosed. The invention task is to obtain objective criteria for the diagnostics of neurosyphilis, provision of early, including pre-clinical diagnostics of the disease, reduction of the terms for obtaining data, reduction of costs for carrying out analyses.
EFFECT: in a number of cases the method is the only one which makes it possible to obtain the valid criteria for diagnostics in the form of a picture of the brain structures destruction, which makes it possible to diagnose neurosiphilis confidently and prescribe specific therapy in due time.
4 dwg, 3 ex
SUBSTANCE: group of inventions relates to medicine, cosmetology, production of food products, vitamins, food supplements, drugs and describes versions of device for realisation of non-invasive potentiometric determination of oxidant/antioxidant activity of biological tissues, which includes device for measuring potentials and double-sided electrode, made in form of plate with similar working surface, covered with electricity-conducting gel, containing mediator system. Electrodes are fixed on biological tissue in such a way that one working surface, playing role of measuring electrode, is in direct contact with biological tissue via gel, second working surface pale role of comparison electrode. Electrodes contact with each other via gel, with oxidant/antioxidant activity being determined by formulae with application of difference between final and initial potentials.
EFFECT: simplification, as well as increase of accuracy and reliability of determination, is achieved.
14 cl, 3 tbl, 4 dwg
SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.
EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.
2 tbl, 3 dwg, 3 ex
SUBSTANCE: test tray comprises a case 1 made of an optically transparent material. From one end face, the case 1 has a stop plug 2 with a hole 3, a connecting pipe 4 threaded to connect to either a haemofilter 5, or a cap 7. The case 1 comprises a movable piston 8 connected by a rod 10 to a handle 11. Electrically supplied electrodes 12 are arranged on surfaces of the stop plug 2, piston 8 and on the inner surface of the case 1. The electrodes 12 are connected to contact groups of a device - a laser analyser - through conductors 13, 14. What is disclosed is an alternative version of the structural embodiment of the test tray.
EFFECT: sterile measurement of the colloidal fluid sample.
17 cl, 5 dwg
SUBSTANCE: technique involves the three-stage diagnosis of all the patients suffering tumour diseases of various localisations. The first stage involves CT-densitometry performed every 6 months; if the CT-densitometry shows sites with varying spine bone density by 30% and more, the second diagnostic stage that involves a transpedicular biopsy is initiated. If the biopsy material appears to contain no tumour material, the third diagnostic stage starts with F-18 positron-emission tomography (PET-CT).
EFFECT: improving the early diagnosis of the spinal tumours.
SUBSTANCE: clinical assessment of the oral mucosa state is ensured by examining non-stimulated oral fluid or swabs. That involves determining five parameters: content of yeast-like fungi Candida in the yeast or mycelia form (1), concentration of secretory immunoglobulin A (SIgA) (2) and lysozyme (3), emission light sum (S) 5 minutes before chemoluminescent examination (4); the Wood-beam beam luminescent examination covers marginal portions of the gum and apexes of interdental papillas, buccal mucosa within dental occlusion, dorsal surface of the tongue within the thread-like processes, and performing morphological examination of the multilayer squamous epithelium of the buccal mucosa along the dental occlusion line (5). The derived results enable diagnosing the absence of pathogenic microflora and oral mucosa pathology, Candida carriage or chronic oral candidiasis in the mycelia or yeast form in the form of hyperkeratosis or leukokeratosis.
EFFECT: using the invention enables increasing the differential diagnostic accuracy for keratolytic processes in the form of white manifestations.
10 dwg, 5 ex
SUBSTANCE: method includes selection, and crushing of biomaterial, two-stage extraction of pesticides with n-hexane, purification of biomaterial from coextractive substances with concentrated sulphuric acid, formation of concentrate of n-hexane extract of pesticides, drying, sample formation by dissolution in 0.5-1 ml of n-hexane and carrying out gaschromatographic identification.
EFFECT: invention is characterised by higher effectiveness and accuracy of research and can be used in biology, ecology, medicine for gaschromatographic identification of organochlorine pesticides, namely α-HCCH, β-HCCH, γ-HCCH, DDT, DDD, DDE, in various biomaterials, such as lipids of internal organs and tissues, blood, milk, bird feathers.
2 cl, 1 dwg
SUBSTANCE: invention represents a method for preclinical study of cardiotropic antiarrhythmic drugs, involving determining the bioelectric parameters in isolated multicellular perfused preparations and measuring an action potential duration, differing by the fact that the isolated multicellular perfused preparations are presented by rat's pulmonary vein myocardium; the parameters are measured in three operation modes of the multicellular preparations; a resting potential is additionally measured; varying APD 90%, related APD 50%/APD 90%, a spontaneous shear velocity of the resting potential, the most positive membrane potential in the resting preparation, a spontaneous activity train repetition rate, spontaneous action potential train repetition and variability frequency, post-depolarisation number and intensity, as well as a shear membrane potential corresponding to the beginning of train activity are used to evaluate the signs of antiarrhythmic and arrhythmogenic action.
EFFECT: more reliable prediction of the antiarrhythmic action of the potential pharmacological agents and reduction of experimental phase time.
SUBSTANCE: method involves examining blood erythrocytes. Blood erythrocyte suspension fluorescence intensity is measured at wavelength of 500 nm polarized along light axis and then at wavelength of 493 nm in perpendicular to the light axis for each polarization direction with following anisotropy value being calculated. Its deviation from norm proves availability of hemolytic disease.
EFFECT: accelerated examination process; reduced risk of traumatic injuries of fetus or newborn.
FIELD: veterinary medicine.
SUBSTANCE: method involves carrying out blood erythrocyte micro electrophoresis in alternating electric field with sign change frequency equal to 0.3-1 Hz. Electric field having current intensity equal to 4.5-5.0 mA is applied. Treatment is applied at 36-38°C. Oscillation amplitude being equal to 3 mm and less, endotoxicosis is detected.
EFFECT: high accuracy and simplicity of the method.
SUBSTANCE: method involves applying integrated diagnostics approach with blood serum examination being applied using infrared spectrometry methods. Sample absorption spectra in the area of 1200-1000 cm-1 are recorded, absorption peak heights having maximum at 1170, 1165, 1140, 1125, 110, 1070, 1025 cm-1 points are measured. Then, mean peak height C and ratio X of peak height at 1125 cm-1 point of maximum to C are calculated. X≥0.7 being the case, light myocardium injury degree is to be diagnosed. 0.7>X≥0.53 being the case, moderate severity degree case is to be diagnosed. X<0.53 indicates grave injury to take place.
EFFECT: high reliability of diagnosis.
FIELD: analytical methods in medicine.
SUBSTANCE: cells of tested organs are subjected to alternating cytological electrophoresis. When cell vibration amplitude rises by at least 20%, drug is regarded to be appropriate for treatment.
EFFECT: increased drug selection efficiency.
FIELD: medicine, hepatology.
SUBSTANCE: the present innovation deals with detecting bilirubin content both before and after curative starvation, moreover, curative starvation one should introduce antipyrine at the dosage of 1 mg/kg, in a day it is necessary to detect antipyrine content in saliva and urine and bilirubin content and at bilirubin content being 30-40 mM/l one should starve at daily intake of 400-450 kcal/d for 3 d, then one should again detect bilirubin level and at its increase by 50-100% one should again introduce antipyrine at the dosage of 1 mg/kg and according to its delayed half-life by 20-50% it is possible to diagnose Gilbert's disease.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, otorhinolaryngology.
SUBSTANCE: the present innovation deals with treating diseases of the upper respiratory tract. One should detect disorders of mucociliary transport (MCT) due to measuring the motor parameters of ciliated epithelium cilia, at least, the rate of mucociliary transport that includes material sampling for further testing as ciliary epithelial cells and registration of cilia fluctuations in human respiratory tract due to TV microscopy performed in patient's lifetime. Material for testing should be sampled from mucosal surface of respiratory tract, and therapeutic tactics should be chosen depending upon motor velocity of cilium's tip obtained based upon mathematical modeling followed by calculations by the formula: , where Vc - velocity of cilium's tip [mcm*sec1]; C1, C2, α - calculated as average values being characteristic for every of human body states under investigation: healthy persons, patients with either acute or chronic forms of sinusitis by the developed model of cilia movement; C1 - curvature of cilium's working length, constant being equal to 1/38 mcm-1; C2 - cilium's curvature from its working length up to the tip [mcm-1]; α - cilium's inclination angle against vertical position [degrees]; π = 3.1417 - constant; L, Ts and Te - parameters measured individually in every patient; L - cilium's length [mcm]; Ts - time from the onset of movement till maximal straightening of cilium's tip [sec]; Te - time of efficient impact [sec]. Moreover, the value being Vc = 9.6 - 11.3 mm/min corresponds to normal MCT functioning. For patients with chronic purulent sinusitis beyond exacerbation accompanied with MCT disorder it is necessary to prescribe complex therapy: surgical or conservative therapy with preparations of secretolytic and secretomotory action. For patients with chronic purulent sinusitis beyond exacerbation without any MCT disorder one should prescribe surgical therapy. All patients with acute sinusitis with MCT disorder should be prescribed complex conservative therapy with preparations of secretolytic and secretomotory action. The method enables to specify optimal therapy based upon scientifically proved indications of investigations by taking into account individual peculiarities of every patient.
EFFECT: higher efficiency.
5 dwg, 3 ex, 2 tbl
FIELD: medical engineering.
SUBSTANCE: device has non-absorbing substrate having hydrophilic target region, which is covered with reagent by applying non-impact micro-drop printing method to produce practically uniform reagent layer. The device is in particular usable for measuring blood coagulation time. Preferential invention embodiment involves determining blood coagulation time by carrying out monitoring of light transition through the target region as the blood sample cover interacts with the reagent.
EFFECT: high reliability of analysis results.
20 cl, 9 dwg
SUBSTANCE: one should register the values of pH and Redox potential (Red) of liquid means in bio-objects simultaneously. It is necessary to plot a graph ▵pH/▵Red according to fluctuations of variation values in measured parameters at their temporal diagram to transfer it into a columnar one where the height of every column is proportional to the area of this graph between neighboring measurements, correspondingly. According to the ratio of area sums of positive columns to that of negative ones during a certain period of time ( from a minute- to a year-long ones) one should conclude upon a state in a bio-object. The value ranged 0.5-2 is considered to be a standard. The method enables to provide efficient control for a two-phase systemic process.
EFFECT: higher efficiency and accuracy of diagnostics.
1 cl, 9 dwg, 5 ex, 1 tbl
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should perform crystallographic study if cerebrospinal fluid and at protein concentration in liquor samples being from 0.50 g/l and higher at predominance of crystals as suppressed dendrites upon a crystallographic picture one should diagnose a malignant cerebral tumor, and at protein concentration being below 0.50 g/l and predominance of crystals as branched dendrites - a benign cerebral tumor. The innovation enables to fulfill not only due diagnostics of cerebral neoplasms, but detect the degree of tumor process malignancy at the early stages of its development.
EFFECT: higher accuracy of differential diagnostics.
3 dwg, 2 ex, 1 tbl
FIELD: biology, medicine, in particular histological investigation of shells with natural surface by using light-optical microscope.
SUBSTANCE: claimed method includes surface relief investigation by using plane field microscope in reflected light at one-side falling shadow-forming lighting. Light-reflecting ability of specimen surface is provided by silver impregnation. Said impregnation is carried out by specimen hold in 10 % silver nitrogen solution for 10 min followed by reducing thereof with 1 % ascorbic acid solution for 1 min.
EFFECT: method for investigation of natural shell surface relief of improved quality due to visual representation of surface three-dimension image on tissue level.
3 cl, 4 dwg