Method of diagnosing staphylococcal allergy in case of allergic rhinitis
SUBSTANCE: functional activity of neutrophilic granulocytes is studied by means of hemiluminiscent analysis, calculated is an index of formation of active forms of oxygen (IFAFO), representing the ratio of the area under the curve of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by a live bacterial suspension of staphylococcus aureus, to the time of achieving the maximum of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by the live bacterial suspension of staphylococcus aureus. If IFAFO value is higher than 100 o.u./s, sensitisation to staphylococcus aureus in patients with allergic rhinitis is diagnosed.
EFFECT: application of the claimed method makes it possible to diagnose staphylococcal allergy in case of allergic rhinitis before beginning therapy and contributes to the optimal selection of the patients' treatment.
1 tbl, 2 ex
The invention relates to medicine, in particular Allergy, immunology, otolaryngology, and can be used for the diagnosis of Allergy to Staphylococcus aureus (S. aureus) in patients with allergic rhinitis.
From literary sources it is known that among patients with perennial allergic rhinitis is quite often marked by the prevalence of Staphylococcus aureus .
Infectious and allergic processes form the basis of the pathogenesis of several diseases, including allergic rhinitis. The study of the etiology and pathogenesis of infectious and allergic rhinitis linked to solving a number of problems, including with regard to the qualitative composition of the microflora of the upper respiratory tract, identification of microbes responsible for sensitization, determine the nature of their allergens. Studies of many authors, in particular SSC Institute of immunology FMBA A. D. ADO, V. N. Fedoseyeva et al. 1997-2003, it was shown that staphylococci play an important role in the pathogenesis infectionsswedish forms of Allergy. He proved the possibility of the formation of immediate hypersensitivity to allergens staph in patients with respiratory allergic diseases and atopic dermatitis. The latter is an important criterion for appointment patients allergenspecific�coy immunotherapy with allergovaccination from Staphylococcus and further tactics of conducting patients with immediate type Allergy to staph.
Identification of the etiological factor of Allergy to date based mostly on the results of skin tests . Setting patch testing is a diagnostic method for detection of specific sensitization of the organism by injecting under the skin of the allergen and evaluating the magnitude and nature developed with edema or inflammatory response. There are different methods skin tests using allergen: skin prick tests, prick, application and intradermal test. For the detection of sensitization to allergens of microbial origin are widely used prick allergiesthe. However, in their formulation often have false-positive results . Currently the experts of the European Academy of Allergology and Clinical Immunology does not recommend the use of prick skin tests to diagnose allergies because of their low information content .
A common drawback of skin testing as a means of Allergy diagnostic is a certain danger to the health of the patient, as it may provoke allergic reactions and additional sensitization.
As a prototype adopted a method for diagnosing staphylococcal allergies, which includes the determination of specific IgE antibodies to Staphylococcus aureus in raw�orode the blood of the patient using active allergenic substance (AAS), isolated from Staphylococcus and consisting of three active fractions with molecular masses of 50, 130 and 170 kDa, while a positive reaction is estimated 2-4 class . The known method allows to detect staphylococcal Allergy in patients with allergic diseases with the author of the test system, however, is time consuming, complex and not widely used in clinical practice.
The object of the invention is the creation of informative method of diagnosis of staphylococcal Allergy in patients with allergic rhinitis.
The task is achieved by using chemiluminescent analysis examined the functional activity of neutrophilic granulocytes in the peripheral blood of patients with allergic rhinitis and calculate the index formation of reactive oxygen species (HIOAFC) by the formula
Sinduced- the value of area under the curve luminol-dependent chemiluminescence of neutrophil granulocytes induced by live suspension of S. aureus relative units (O. E.);
Tmax- time to a maximum of luminol-dependent chemiluminescence of neutrophil granulocytes induced by live bacterial suspension of S. aureus, seconds (s).
If the value of IOAFCmore than 100 O. E./diagnosed with sensitization to S. aureus in ill�x allergic rhinitis.
The threshold value of 100 OE/s obtained empirically on the basis of the comparison data IOAFCobtained by chemiluminescent method in the study of the functional activity of neutrophilic granulocytes in the peripheral blood of patients with allergic rhinitis, with the results of skin tests to these patients for the presence or absence of sensitization to S. aureus.
Activated neutrophils are potent effectors and trigger mechanisms of the cascade of reactions that ensure the development of inflammation. Anti-infective action of neutrophil granulocytes is associated primarily with the generation of reactive oxygen species, and one of the methods allowing to evaluate the oxygen-dependent biocenose neutrophil granulocytes, is chemiluminescent analysis.
Neutrophil granulocytes are capable of killing microorganisms using oxybiotic mechanism, which includes "respiratory burst" that develops in the interaction of neutrophils with the objects of phagocytosis. "Respiratory burst" refers to a series of metabolic processes that occur during stimulation of neutrophils: increased oxygen consumption and increased glucose oxidation in the pentose phosphate cycle (TFG), and as a result, the production of reactive oxygen species, possessing a sh�m action. The functional state of phagocytic cells can be described through the level of "respiratory explosion" in a sharp increase in the consumption of oxygen by converting it into an active form. The ability of neutrophilic granulocytes to form the desired amount of ROS may serve as prognostic sign to assess the further course of the inflammatory process, and the level of response to the inductor "respiratory explosion" can characterize the activity of the body's defenses. On reception intensity "respiratory explosion" was founded many clinical diagnostic methods for assessing the condition of the body, and in particular chemiluminescence assay (CL). The rate of formation of ROS does not depend on the sensitivity of the chemiluminescent analyzer, which conducted the study.
The method is as follows.
From venous blood of a patient allergic rhinitis with suspected bacterial Allergy (with prolonged ineffective anti-allergic therapy in perennial allergic rhinitis) was isolated neutrophilic granulocytes. To 5 ml of blood with heparin was added 1 ml polyglucin and incubated for 30 min at 37°C to accelerate the sedimentation of erythrocytes. The supernatant layer on a double density gradient fillmemory (ρ=1,077 for the isolation of lymphocyte populations; ρ=1,199 for the isolation of a population of neutrophilic granulocytes) and centrifuged at 400 g for 45 minutes. In the control of morphological composition of leukocyte suspensions determine the output purity of neutrophil granulocytes, which is not less than 97%. The resulting suspension of neutrophil granulocytes washed twice in Hanks solution without phenol red for 10 min at 400 g. The supernatant is decanted, the remaining neutrophil granulocyte diluted in 1 ml of Hanks solution and get suspended. Count the number of neutrophil granulocytes in the camera Goryaeva. For conducting chemiluminescent analysis using the following reagents: donor serum (blood group AB, RH factor negative), the solution Hanks (without phenol red), luminol at a concentration of 100 μg/ml. Prepared sample: 200 μl of a suspension of neutrophil granulocytes, 20 µl of the donor's serum, 240 μl of Hanks solution, 50 μl of luminol and 40 µl of live suspensions of Staphylococcus aureus at a concentration of 106CFU/ml. Measuring the chemiluminescent response is carried out using a chemiluminescent analyzer, for example "CL3604", for 90 minutes (5400 s). Results registration and management of the chemiluminescent analyzer was carried out in a computer. Get curve luminol-dependent chemiluminescence of neutrophil granulocytes induced live suspended platforms�S. iy aureus. Calculated by the formula for the index of education AFK. Depending on the values of the calculated index to determine the presence or absence in patients with allergic rhinitis sensitization of Staphylococcus aureus. The value of IOAFCmore than 100 OE/s indicates sensitization of the patient to S. aureus, and the patient is recommended the sowing of the flora and antibiogram. HIOAFCequal to or less than 100 OE/sec indicates the absence of sensitization to S. aureus.
The inventive method was tested on 45 patients with allergic rhinitis received treatment in the Department of otolaryngology of ngha "railway clinical hospital at the station Krasnoyarsk OJSC RZD. The results of the survey are presented in table 1. 15 patients allergic rhinosinusitis was defined HIOAFCabove 100. Cutaneous allergic samples showed the presence of bacterial Allergy. 3 patients had allergic rhinitis IOAFCwas below 100, but skin allergic samples showed the presence of bacterial Allergy. In 21 cases of allergic rhinitis was noted HIOAFCbelow 100, and cutaneous allergic samples showed the presence of Allergy to animal hair. In 6 patients noted HIOAFCbelow 100, and skin Allergology and ELISA for specific IgE revealed the presence of seasonal Allergy to pollen. Thus, the coincidence RES�objectives of Allergy diagnostic for staph in allergic rhinitis inventive method is 94%.
|The results of the study sensitization to S. aureus in patients with allergic rhinitis by the inventive method and the results of skin tests|
|№ p/p||HIOAFC||Sensitization to S. aureus claimed method||Sensitization to S. aureus according to the results of skin tests||The coincidence of forecast|
|3||RUB 220.3||Should be||There||-|
|12||To 128.3||Should be||There||+|
|14||To 244.7||Should be||There||+|
Clinical example 1. Patient G., age 42. In 2008, diagnosed with allergic rhinitis. Taking anti-allergic therapy without effect. Aggravation of allergic rhinitis was observed in autumn-winter period. In the history�, frequent respiratory infections, which preceded or accompanied the allergies. Total IgE in the serum equal to 170 IU/ml) Specific IgE to pollen, animal dander and foods showed negative values. Held-resolved diagnostics Staphylococcus aureus inventive method. HIOAFC$ 125 O. E./s (more than 100 OE/s). The patient was diagnosed with sensitization to S. aureus. Recommended the appointment of specific immunotherapy (SIT) Staphylococcus aureus.
Clinical example 2. Patient A., 50 years. In 1970, diagnosed with allergic rhinitis. Taking anti-allergic therapy without effect. Aggravation of allergic rhinitis occurs after a cold. Total IgE in the serum equal to 220 IU/ml) Specific IgE to pollen, animal dander and foods showed negative values. Held-resolved diagnostics Staphylococcus aureus inventive method. HIOAFCa total of 512 O. E./s (more than 100 OE/s). In a patient diagnosed with sensitization to S. aureus. Recommended the appointment of the SITH Staphylococcus aureus.
The technical result from implementation of the proposed method:
- possibility of diagnosis of sensitization to S. aureus;
- lack of subjective factors in evaluating the results of diagnostics of staphylococcal sensitization;
- expanding Arsenal of methods of di�Gnostics staph Allergy in allergic rhinitis.
Thus, the method is informative, meets the modern requirements to the methods of laboratory diagnostics allows to detect staphylococcal sensitization before therapy and promotes optimal treatment of patients with allergic rhinitis.
Sources of information
1. Fedoseyev V. N., Kolotilov B. A., Larina, O. N., Fedotova T. G. Bacterial Allergy. Penza, private printing Tugusheva, 2004.
2. Picky V. I., Hadrian N. In., Artamonova V. A. Allergic diseases. Moscow: Triada-X, 1999. P. 102-112.
3. Gushchin I. S. Allergic inflammation and its pharmacological effects. M.: Farmacos The Priit, 1998.
4. Fedotova T. G. et al. Principles of diagnosis of allergic diseases. // Consilium medicum. - 2002. - No. 4. - P. 13-19.
5. Pat. 2436100 of the Russian Federation. Method of determination of specific IGE antibodies to the Staphylococcus in the blood serum of a patient allergic diseases with hypersensitivity to allergens staph, using the test system for the detection of specific IGE antibodies to the Staphylococcus isolated from Staphylococcus active allergenic substance (AAS) and a method for diagnosing staphylococcal Allergy / Fedoseyev V. N., Kamyshev V. A. and others; applicant and patentee: Institute of immunology" FMBA of Russia (the Russian Federation). - No. 2010111254/15; Appl. 25.03.2010; publ. 20.09.2010.
A method for diagnosing staphylococcal Allergy in allergic �inite,
includes the study of blood, characterized in that by using chemiluminescent analysis examined the functional activity of neutrophil granulocytes in peripheral blood of patients and calculate the index formation of reactive oxygen species (HIOAFC) by the formula
Sinduced- the value of area under the curve luminol-dependent chemiluminescence of neutrophil granulocytes induced by live suspension of Staphylococcus aureus (S. aureus), relative units (O. E.);
Tmax- time to a maximum of luminol-dependent chemiluminescence of neutrophil granulocytes induced by live bacterial suspension of S. aureus, seconds (),
and if the value of IOAFCmore than 100 O. E./diagnosed with sensitization to S. aureus in patients with allergic rhinitis.
SUBSTANCE: invention represents a method for determining a probability of preserving the myocardium following infarction by creating an admission examination-based data array of 7 peripheral blood parameters, 11 biochemical analysis parameters and 6 parameters of standard 12-lead electrocardiogram in 200 patients with the Q-myocardial infarction and 200 patients without the myocardial infarction. The parameters are stratified with respect to 7 intervals, wherein values related to the probability of preserving the myocardium following infarction are derived by calculating a ratio of the patients without myocardial infarction to all the patients with acute coronary syndrome. The probability is evaluated in a specific patient by analysing the above parameters, searching the respective intervals and values related to the probability of preserving the myocardium in the data array. Summing up the derived values enables calculating an integrated index, which is normalised, and a dimension from 0 to 100% is reduced.
EFFECT: invention enables increasing the prediction accuracy of preserving the myocardium in the patients with acute coronary syndrome.
1 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine, namely to immunology, and can be used in laboratory diagnostics, as a test system and a method for measuring interferon alpha (IFN-α) antiviral activity in human blood serum. The test system for measuring IFN-α activity in the human blood serum comprises diploid cells, a virus-containing fluid and standard human interferon-α (IFN-α). As the diploid cells, the test system comprises cells of the characterised line of the diploid cells - human M-20 fibroblasts at 20-33 passages cultured in the medium with added 10% fibrinolytically active plasma (FAP). As the virus, the system contains the A vesicular stomatitis virus (VSV) adapted to M-20 cells, the Indiana strain; the test system additionally contains a viral dye based on two fluorochromes - acriflavine and rhodamine C. The group of inventions also involves a method for measuring IFN-α activity in the human blood serum with the use of the developed system.
EFFECT: using the given inventions enables the quantitative good-reproducible measurement of IFN-α activity in the examined blood serum sampled by the luminescent microscopy of the vital preparations coloured in a fluorochrome-based vital dye.
3 cl, 4 dwg, 1 tbl, 4 ex
SUBSTANCE: invention describes a diagnostic technique for infected pancreonecrosis with establishing the indications for a surgical intervention by examining a patient, wherein acetic, propionic, butyric and isovaleric acids are measured in the blood by gas chromatography, and if the acetic acid concentration is more than 0.11 mmole/l enables stating infected pancreonecrosis, and if the concentration of any of three acids: propionic more than 0.095 mmole/l, butyric more than 0.0035 mmole/l, isovaleric acid more than 0.0003 mmole/l enables stating infected pancreonecrosis with active development of an anaerobic infection requiring one of versions of the surgical intervention.
EFFECT: invention enables providing the objective and accurate diagnosing of pancreonecrosis transformation into the stage of infection by using the qualitative parameters with no undesired side effects.
SUBSTANCE: invention relates to medicine, namely to a method of predicting a probability of reduction of the glomerular filtration rate (GFR) after 3 months of observation after aortocoronary bypass surgery without artificial blood circulation (ACBS without ABC). The essence of the method consists in the fact that the concentration of a kidney injury molecule of type 1 (KIM-1) is determined in blood serum, the ratio of the biomarker KIM-1 concentrations in two time points after 48 hours and 7 days after the operation is calculated and if its value is higher than 1.5, a conclusion about the probability of (GFR) reduction in the remote period after ACBS without ABC is made.
EFFECT: application of the claimed method makes it possible to predict the probability of the glomerular filtration rate (GFR) reduction after 3 months of observation after aortocoronary bypass surgery without artificial blood circulation in an efficient and accurate way.
1 tbl, 1 dwg, 1 ex
SUBSTANCE: method includes determination of a titre of antibodies to cytomegalovirus, content of 2,3 DPG (2,3-diphosphoglycerate), and oxyhaemoglobin in erythrocytes. If the titre of the antibodies to cytomegalovirus increases to 1:1600, DPG increases to 6.7±0.3 mcmole/ml, content of HbO2 is 95.0±1.7%, with the reduction of the specific optical density of haemoglobin to 0.70±0.01, it is concluded that a threat of anaemia development is formed.
EFFECT: method makes it possible to study the character of haemoglobin oxygenation impairment by means of determination of the specific optical density.
SUBSTANCE: neurovisualisation examination of brain is carried out, Cirs comorbidity index and Kaplan-Feinstein comorbidity index are determined, cochleovestibular syndrome, eye-moving impairments, type of diabetes mellitus are identified. Value of discriminate function (D) is calculated. If D value is higher than zero, diagnosed are consequences of ischemic brain stroke (IBS) with hyperhomocysteinemia (HH), if D is lower than zero, consequences of IBS without HH are diagnosed.
EFFECT: method makes it possible to increase reliability of diagnostics of IBS consequences, which is achieved due to complex analysis of said parameters.
FIELD: veterinary science.
SUBSTANCE: method includes detection of leukocytes, protein, haemoglobin in faeces, and PH-reaction of faeces. A sample of faeces is dissolved in distilled water and applied drop by drop onto appropriate test fields of a test strip designed for urine testing. By variation of colour within 1 minute the reaction is assumed as positive. If pH is less than 7.0 or more than 7.5, and faeces contain soluble protein, haemoglobin and leukocytes at the same time, availability of the inflammatory process in the intestines is confirmed.
EFFECT: invention makes it possible to quickly and accurately diagnose inflammatory process in intestines.
2 cl, 2 ex
SUBSTANCE: invention refers to medicine and aims at diagnosing vaginitis in females of a reproductive age. Expression levels of mRNA of TNF, IL18, GATA3 and TLR4 genes are measured in vaginal smears. Expression levels of mRNA of TLR4/GATA3 and TNF/IL18 genes are related, and the derived threshold cycles are used to predict a probability of vaginitis by formula. If P≤57% for nonpregnant females or P≤68.5% for pregnant females, the absence of vaginitis is stated. If P≥57% for nonpregnant females or P≥68.5% for pregnant females, vaginitis is diagnosed.
EFFECT: invention provides the effective diagnosis of vaginitis in the females of a reproductive age.
3 tbl, 5 ex
SUBSTANCE: group of inventions refers to medicine, namely to a method of analysing ex-vivo if a patient suffering a cancer is expected to respond therapeutically to a method of treating. To this purpose, a biological sample specified in a group consisting of total blood, plasma or serum samples is taken from the patient. The levels IL10 and IFNγ are measured in the above biological sample; the levels are determined with using the respective IL10 and INFγ specific antibodies. The IL10/IFNγ ratio is derived and compared to a threshold. If the IL10/IFNγ ratio of less than 4 shows that the patient is expected to develop the preventive or therapeutic response to the immunogenic composition. The group of inventions also refers to using IL10 and INFγ as biomarkers and a kit for analysing the above method.
EFFECT: using the given method enables predicting the sensitivity of the patient suffering cancer to the therapeutic treatment, as well as obtaining an algorithm for treatment modification for improving the patient's response to the treatment.
12 cl, 2 dwg, 1 ex
SUBSTANCE: invention deals with early diagnostics of infectious complications in patients with acute lymphoblastic leukaemia (ALL), obtaining chemotherapy (CT). Claimed method consists in the following: concentration of fibrinogen, time of XIIa-dependent euglobulin lysis (XIIa-DEL), level of soluble fibrin-monomer complexes (SFMC), activity of protein C (prC) are analysed in ALL patients during CT 2-3 times per week. If at least 2 of 4 criteria are detected, namely: ≥41% increase of fibrinogen level, ≥76% increase of SFMC, ≥11% prolongation of XIIa-DEL time and ≥12% reduction of prC activity in comparison with previous analysis, development of infectious complications is predicted.
EFFECT: method provides early prediction of infection development in patients with acute lymphoblastic leukaemia during chemotherapy.
7 tbl, 4 ex
FIELD: medicine, hepatology.
SUBSTANCE: one should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, infectology, hepatology.
SUBSTANCE: in hepatic bioptate one should detect products of lipid peroxidation (LP), such as: dienic conjugates (DC), activity of antioxidant enzymes, such as: catalase (CAT)and superoxide dismutase (SOD). One should calculate by the following formula: C = DC/(SOD x CAT)x100, where DC - the content of dienic conjugates, SOD - activity of superoxide dismutase, CAT - activity of catalase. At coefficient (C) values being above 65 one should predict high possibility for appearance of cirrhosis, at 46-645 - moderate possibility and at 14-45 -low possibility for appearance of cirrhosis.
EFFECT: higher accuracy of prediction.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: clinical medicine, pulmonology.
SUBSTANCE: one should carry out complex estimation of interleukin-1β) concentration in blood, saliva, bronchoalveolar liquid. Moreover, one should detect distribution coefficient (DC) for IL-1β as the ratio of IL-1β blood content to IL-1β salivary content. At increased IL-1β blood content by 10 times and more, by 2 times in saliva, unchanged level of bronchoalveolar IL-1β, at DC for IL-1β being above 1.0 one should predict bronchial obstruction. The method enables to conduct diagnostics of the above-mentioned disease at its earlier stages.
EFFECT: higher efficiency of prediction.
FIELD: medicine, diagnostics.
SUBSTANCE: the present innovation deals with genetic trials, with diagnostic field of oncological diseases due to analyzing DNA by altered status of gene methylation that take part in intracellular regulation of division, differentiating, apoptosis and detoxication processes. One should measure the status of methylation in three genes: p16, E-cadherine and GSTP1 in any human biological samples taken out of blood plasma, urine, lymph nodes, tumor tissue, inter-tissue liquid, ascitic liquid, blood cells and buccal epithelium and other; one should analyze DNA in which modified genes of tumor origin or their components are present that contain defective genes, moreover, analysis should be performed due to extracting and purifying DNA out of biological samples followed by bisulfite treatment of this DNA for modifying unprotected cytosine foundations at keeping 5-methyl cytosine being a protected cytosine foundation followed by PCR assay of bisulfite-treated and bisulfite-untreated genes under investigation and at detecting alterations obtained according to electrophoretic result of PCR amplificates, due to detecting the difference in the number and electrophoretic mobility of corresponding fractions at comparing with control methylated and unmethylated samples containing normal and hypermethylated forms of genes one should diagnose oncological diseases. The method provides higher reliability in detecting tumors, detection of remained tumor cells after operation.
EFFECT: higher efficiency of therapy.
1 cl, 3 dwg, 4 ex
FIELD: medicine, gastroenterology.
SUBSTANCE: one should carry out diagnostic studying, moreover, on the 5th -6th d against the onset of exacerbation in case of gastric and duodenal ulcerous disease one should detect the content serotonin, histamine and acetylcholine in blood, then during 2-3 wk one should conduct medicinal therapy to detect serotonin, histamine and acetylcholine level in blood again and at serotonin content being by 2-3 times above the norm, histamine - by 1.15-1.4 times above the norm and acetylcholine - by 20-45% being below the norm one should predict the flow of gastric and duodenal ulcerous disease as a non-scarring ulcer.
EFFECT: higher accuracy of prediction.
SUBSTANCE: method involves taking blood from ulnar vein (systemic blood circulation) and from large vein of the injured extremity proximal with respect to lesion focus (regional blood circulation). Spontaneous NST-test value is determined and difference is calculated in systemic and regional blood circulation as regional-to-systemic difference. The difference value is used for predicting clinical course of pyo-inflammatory disease in extremities.
EFFECT: high accuracy of diagnosis.
4 cl, 2 tbl
FIELD: medicine, gastroenterology.
SUBSTANCE: one should introduce biologically active substance, moreover, in patient's blood serum one should detect the content of acetyl choline and choline esterase activity followed by 2-h-long intragastric pH-metry at loading with biologically active substance as warm 40-45%-honey water solution at 35-40 C, and at increased content of acetyl choline being above 1.0 mM/l, choline esterase being above 0.5 mM/l/30 min and pH level being 6.0-6.9 it is possible to consider apitherapy to be useful for treating ulcerous duodenal disease.
EFFECT: higher efficiency and accuracy of detection.
FIELD: medicine, gastroenterology.
SUBSTANCE: it has been suggested a new method to detect pharmacological sensitivity to preparations as acidosuppressors. After the intake of the preparation a patient should undergo fibrogastroduodenoscopy 3 h later, then, through endoscopic catheter one should introduce 0.3%-Congo red solution intragastrically and the test is considered to be positive at keeping red color that indicates good sensitivity to the given preparation, and in case of dark-blue or black color the test is considered to be negative that indicates resistance to this preparation. The suggested innovation widens the number of diagnostic techniques of mentioned indication.
EFFECT: higher efficiency of diagnostics.