Method of obtaining porous chitosan calcium phosphate-containing sponges for filling bone defects
SUBSTANCE: described is a method of obtaining a composite chitosan-based material, which contains aspartic or glutamine amino acids in a quantity from 2 to 5 wt %, as well as calcium phosphates with a ratio of Ca/P from 1.0 to 1.67. The method consists in barbotage through a suspension of calcium phosphates, obtained in situ in a solution of chitosan and aspartic or glutamic acid, with the following lyophilic drying of the foamed products. Porous matrices can be applied in dentistry, maxillofacial surgery, osteoplastic surgery as implants in the treatment of bone tissue defects.
EFFECT: obtained samples are characterised by a uniform porous structure with the simultaneous reduction of calcium phosphate dimensions to a nano-level with the reduction of the number of material obtaining operations.
2 dwg, 5 ex
The invention relates to composite materials for medicine, namely traumatology and Orthopaedics, maxillofacial surgery and surgical dentistry, and can be used for the manufacture of consolidated materials for filling bone defects.
Because the bone tissue is a composite material containing calcium phosphate (FC) and organic components (collagen, collagen biokompozitnyh produce and proteins), such a composition allows you to carry the mechanical loads, which are critical, for example, for ceramic bone implants. So promising is the use of composite materials containing both inorganic (FC), and organic components. In addition to collagen and gelatin as the organic component can be used chitosan. Chitosan is a biocompatible and biodegradable natural polymer, which allows its use in various fields of medicine, including for fast healing of different etymologies (Chitin and chitosan. Preparation, properties and application. Under the editorship of academician of the RAAS, K. G. Skryabin. Science. 2002. 365 p.). Especially widely used chitosan materials in the form of a plastic porous sponges. Due to the porosity of these materials are easily deformed to the desired RA�measure (bone defect) and after placing them in a compressed state in the bone defect straightened (due to the inverse deformation), filling the defect volume. Chitosan refers to polysaccharides, substances that promote the formation of bone tissue. However, chitosan sponges do not contain such important for the formation of bone elements, such as phosphorus and calcium. Therefore, when resorption such sponges in the bone defect formed mainly of cartilage (chondroitine). Calcium phosphates, such as dicalciumphosphate (DCPD), octacalcium phosphate (OCP), tricalcium phosphate (TCP), amortizatory calcium phosphate (ROS), precipitated hydroxyapatite (RSA), carbonate-bearing hydroxyapatite (KGA), other substituted forms of hydroxyapatite (HA), as a result of biodegradation in the human body under the action of biological fluids to form calcium ions and phosphate, contributing to the formation of bone tissue de novo.
In the prior art closest to the proposed technical solution and the achieved effect of the invention: RF patent №2376019 "Porous composite material based on chitosan and gelatin containing octacalcium to fill bone defects. Porous chitosan sponges were prepared as follows: powder of high molecular weight chitosan (molecular weight 450000-500000 g/mol) in 1 g of 33.3 wt.%) was dissolved in aqueous acetic acid. Then added with stirring 1 g of 33.3 wt.%) the HA granules (filler)with a grain size of 100-300 μm and 1 g of 33.3 wt.%) powder of ammonium carbonate. The result was plastic composite sponge with a porosity of 85%. The disadvantages of this invention are the necessity of using in the process of obtaining sponges acetic acid and ammonium carbonate, and also a large number of operations prior to the receipt of a porous chitosan sponges: synthesis of FC, their drying, disaggregation, molding and sintering the obtained FC materials.
In the patent of Russian Federation №2376019 C2 "Porous composite materials based on chitosan for filling of bone defects has been consolidated materials from porous composite matrix based on chitosan and FC (KMHFQ). Powder of high molecular weight chitosan (molecular weight 450000-500000 g/mol) in 1 g of 33.3 wt.%) was dissolved in acetic acid solution. Then under stirring was added 1 g of 33.3 wt.%) the HA granules (filler) with a grain size of 100-300 μm and 1 g of 33.3 wt.%) powder of ammonium carbonate. The resulting sponge was washed with ethanol and air dried to remove ethanol. The result was plastic composite sponge with a porosity of 85%. The disadvantage of the above invention are the use, for the expansion of ammonium carbonate and acetic acid, resulting in the formation of a byproduct, ammonium acetate, and the need for surgery washing from him.
The object of the present invention is the crea� biocompatible material, approximate in structure to natural bone tissue, while reducing the number of operations of receipt of materials.
The technical result of the present invention is to maintain the porosity of the material while reducing the size of the PC to nanorana (less than 100 nm) to reduce the number of operations of receipt of materials.
The technical result is achieved in that the porous chitosan sponge for filling bone defects containing FC, obtained by synthesis in situ FC in an aqueous solution containing chitosan and amino acid (glutamic or aspartic), taken in an amount of 2-5 wt.% when the temperature of the reaction mixture from 37 to 90°C, according to the invention, 30 minutes after the completion of the synthesis the reaction mixture was dried by bubbling air through it using compressor, after which the foam mixture is dried by freezing in freeze-dryer.
The essence of the invention consists in the synthesis of FC in situ in a solution containing chitosan, aspartic or glutamic acid, with the subsequent foaming of the slurry and drying it in freeze dryer. This method of obtaining porous chitosan matrices eliminates several operations - filtration step FC, drying, disaggregation (grinding in a planetary or ball mill) and a uniform distribution in the solution of chitosan and �asparaginovoi or glutamic acids. In addition, the proposed in the invention method for producing porous matrices allows to keep the size of the particles of calcium phosphates in chitosan matrix at the level of 30-70 nm, as in natural bone tissue. Nanorazmernoi particles FC in chitosan matrix is saved due to the absence of the drying process the received FC at which the agglomeration of particles and the formation of dense agglomerates and durable large aggregates.
During freeze-drying of foamed samples is the removal of water, in which water from a solid turns into a gas, bypassing the liquid state. This preserves the porous structure of the foamed sample.
2 g of aspartic acid was dissolved in 100 ml of water, then in the resulting acid solution (pH 3.5 to 4.0) was dissolved 2 g of chitosan of high molecular weight (M. 300000 kDa) at 37°C. To the resulting solution was added 40 ml of a solution of calcium nitrate 1 mol/l, and then to the solution was added with stirring dropwise 40 ml of a solution of dibasic ammonium phosphate in a concentration of 1 mol/L. pH of the mixture at the end of synthesis at 5.5-6.0. 30 minutes after start of mix through suspensions were barbotirovany the air using a compressor. The foam mass was placed in the form, then produced the drying with the use�of freeze dryers. The main phase according to the XRD is DCPD, the diffraction patterns are also present in the band corresponding to aspartic acid and chitosan. The particle size of FC in chitosan matrix is in the range of 20-70 nm. The porosity of the material is in the range of 80-85%, the pores are interconnected.
6 g of aspartic acid was dissolved in 100 ml of water, then in the resulting acid solution (pH 3.5 to 4.0) was dissolved 6 g of chitosan of high molecular weight (M. 300000 kDa) at a temperature of 60°C. Formed a very viscous suspension. When you add 60 ml of a solution of calcium nitrate solution with the slurry is not mixed, there was a formation of a homogeneous solution. After adding ammonium phosphate precipitate formed, which was unable to evenly distribute the suspension volume of chitosan. When you try to barbotirovany air through the reaction mixture the passage of air bubbles through the suspension proved to be impossible due to too high viscosity of the latter.
1 g of aspartic acid was dissolved in 100 ml of water, then in the resulting acid solution (pH 2.5 to 3.0) was dissolved 1 g of chitosan of high molecular weight (M. 300000 kDa) at a temperature of 90°C. To the resulting solution was added 50 ml of a solution of calcium nitrate 1 mol/l, and then to the solution was added with stirring dropwise 30 ml of a solution of disodium FOS�ATA ammonium concentration of 1 mol/L. the pH of the mixture during the synthesis process was maintained in the range of 8.5-9.0 by the addition of ammonia solution. After 30 minutes of sedimentation, the suspension was foamed by bubbling air using a compressor. After foaming was too rapid settling of the foam, which is not given the opportunity to perform lyophilization preserving the structure of the foam. As a result of drying instead of a sponge formed powder due to low concentration of chitosan and consequently low viscosity suspension.
5 g of glutamic acid was dissolved in 100 ml of water, then in the resulting acid solution (pH of 4.0) was dissolved 5 g of chitosan of high molecular weight (M. 300000 kDa). To the resulting solution was added 60 ml of a solution of calcium nitrate 1 mol/l, and then to the solution was added with stirring dropwise 40 ml of a solution of disodium phosphate ammonium concentration of 1 mol/L. pH of the mixture during the synthesis process was maintained at 7.0 by the addition of ammonia solution. 30 minutes after start of mix through suspensions were barbotirovany the air using a compressor. The foam mass was placed in the form, then produced the drying using freeze dryers. The main phase according to the XRD is AFK, are present in diffraction patterns of the bars corresponding to glutamic sour�e and chitosan. The particle size of FC in chitosan matrix is in the range of 20-70 nm. The porosity of the matrix was 70-80%, the pores are interconnected. In Fig. 1(a, b) presents the microstructure of the sample, confirming it.
4 g of aspartic acid was dissolved in 100 ml of water, then in the resulting acid solution (pH 2.5 to 3.0) was dissolved 4 g of chitosan of high molecular weight (M. 300000 kDa) at a temperature of 90°C. To the resulting solution was added 50 ml of a solution of calcium nitrate 1 mol/l, and then to the solution was added with stirring dropwise 30 ml of a solution of disodium phosphate ammonium concentration of 1 mol/L. pH of the mixture during the synthesis process was maintained in the range of 8.5-9.0 by the addition of ammonia solution. After 30 minutes of sedimentation, the suspension was foamed by bubbling air using a compressor. After foaming the slurry was dried in freeze dryer. The main phase according to the XRD is RSA, which is confirmed by the strongly broadened peaks in diffraction patterns (Fig. 2), there are also bars corresponding to aspartic acid and chitosan. The particle size of FC in chitosan matrix is in the range of 60-90 nm. The porosity of the material is in the range of 80-85%, the pores are interconnected.
A method of producing a porous chitosan sponge containing calcium phosphates, for filling bone defects, ex�featuring the the in situ synthesis of calcium phosphates in aqueous solution containing chitosan and glutamic or aspartic acid, taken in an amount of 2-5 wt.%, carried out at a temperature of the reaction mixture from 37 to 90°C, 30 minutes after the completion of the synthesis the reaction mixture was dried by bubbling air through it using compressor, after which the foam mixture is dried by freezing in freeze-dryer.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, namely to a polymer composition for medical devices containing polycarbonate with a degree of polymerisation of n=200-2,000 in an amount of 100 weight fractions, a polymer additive, which is presented by polysulphon at a degree of polymerisation of n=70-150, in an amount of 5 to 40 weight fractions, a complex stabiliser, which is presented by sterically hindered phosphite in an amount of 0.045 to 1.5 weight fractions, a compatibiliser representing maleinised polypropylene in an amount of 0.025 to 5.0 weight fractions, a nanostructured additive representing carbon nanotube superconcentrate with the nanotube content of 20-40 weight fractions in butadiene oligomer with a degree of polymerisation of 6 in an amount of 0.01 to 1.0 weight fractions.
EFFECT: invention provides creating the polymer composition with high strength characteristics.
3 cl, 1 ex, 2 tbl
SUBSTANCE: invention represents a method of medication encapsulation by a method of non-solvent precipitation, which differs in the fact, that as a nanocapsules core is applied chondroitin sulphate, as a casing - konjac gum, which is precipitated from a suspension in butyl alcohol due to hexane addition as a non-solvent at 25°C.
EFFECT: simplification and acceleration of the nanocapsules obtaining process, reduction of loss at nanocapsules obtaining.
SUBSTANCE: invention represents method of medication encapsulation by method of precipitation with non-solvent, characterized by the fact that as core of nanocapsules albendazole is applied, as casing - sodium alginate, which is precipitated from suspension in butanol by addition of chloroform as non-solvent at 25°C.
EFFECT: simplification and acceleration of nanocapsules obtaining process, reduction of loss in obtaining nanocapsules.
SUBSTANCE: core of nanocapsules of chondroitin sulphate and the envelope as carrageenan is used, which is deposited from a suspension in butyl alcohol by adding hexane as a nonsolvent at 25°C.
EFFECT: simpler and faster process of producing nanocapsules and reduced losses when producing nanocapsules.
SUBSTANCE: in a tunnel field effect transistor with an insulated gate containing electrodes of source and drain made of multilayer graphene and located at an insulating substrate in the same plane, and also the gate made of a conducting material and located above the areas of source, tunnel junction and drain, electrodes of source and drain are oriented towards each other crystallographically by an even edge of a zigzag type and separated by a vacuum barrier transparent for charge carriers.
EFFECT: invention expands the inventory of tunnel transistor nanodevices; this device alongside its pronounced switching property has on the current-voltage curve of the source and drain electrodes the area with a negative differential resistance, which allows its functioning as the Gunn diode; the device requires lower voltage at the gate.
FIELD: physics, photography.
SUBSTANCE: multiple-junction photoelectric device comprises first and second electrodes, a photoelectric stack in electrical contact with said first and second electrodes and having a plurality of photoelectric junctions, wherein each said photoelectric junction includes an electron-acceptor semiconductor layer and a light-absorbing semiconductor layer, having a substantially high work function than said electron-acceptor semiconductor layer, wherein said photoelectric junctions are divided by a recombination region which includes a transparent and current-conducting hole layer in ohmic contact with said light-absorbing semiconductor layer of said first photoelectric junction, and a transparent current-conducting electron-acceptor layer in ohmic contact with said electron-acceptor semiconductor layer of said second photoelectric junction; said recombination region forms a gradient work function of said transparent and current-conducting hole layer in ohmic contact with said light-absorbing semiconductor layer of said first photoelectric junction to said transparent and current-conducting electron-acceptor layer in ohmic contact with said electron-acceptor semiconductor layer of said second photoelectric junction, and having thickness in the range of one order of the sum of the Debye length of all layers of said recombination region.
EFFECT: invention improves the conversion efficiency of photoelectric cells by providing a low-energy path for recombination of electron and hole currents from pairs of photoelectric junctions.
33 cl, 10 dwg, 6 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions relates to medicine, namely to dermatology and mycology, and can be applied in the treatment of skin and its appendages. A pharmaceutical composition for external application contains nanoparticles for the laser thermotherapy of infectious affections of the skin and its appendages. The nanoparticles are characterised by, at least, one localised surface Plasmon resonance in the range of a wavelength from 400 to 1100 nm. The nanoparticles are dispersed in a physiologically acceptable carrier, which is characterised by the absence of the absorption or weak absorption and/or weak dispersion of light radiation in the said range of wavelengths and possessing biocidal properties. The pharmaceutical composition is applied on an affected area and irradiated by laser radiation with a wavelength close to the wavelength of the localised surface Plasmon resonance of the nanoparticles, contained in the composition, or equal to it. The irradiation is continued until the desirable temperature of heating of the said area is achieved.
EFFECT: group of inventions ensures an increased treatment efficiency, reduction of a risk of development of side effects, reduction of the number of recurrences due to the application of the pharmaceutical composition, capable of absorbing energy of the light radiation and transforming it into heat energy with the achievement of the specified temperature with laser irradiation at the specified wavelength with the lower intensity of laser radiation and possessing biocidal properties.
63 cl, 3 dwg, 1 tbl, 4 ex
SUBSTANCE: at a core (1) made of a steel wire layers of current-conducting wires of two types are wound - aluminium wire (2) and nanocomposite wire (3). The layers of wire (3) are alternated with the layers of wire (2). The layer of wire (3) is wound to the core (1). The wire (3) is formed of a wire stock (wire rod) produced of a nanocomposite material based on aluminium with a nanoparticle filler made as multilayer carbon nanotubes and reinforced in the process of multiple cold drawing of the wire stock up to the preset diameter.
EFFECT: increased capacity, mechanical strength and resistivity to sagging without an increase in its weight.
2 cl, 1 dwg
SUBSTANCE: invention relates to a microbubble generator and to a device for microbubble generation. One of the aspects of the claimed invention is represented by the microbubble generator, containing a vortex chamber, an opening for the supply of a fluid medium, connected to the vortex chamber, with the opening for the fluid medium supply being intended for the supply of the fluid medium along the line, which is tangent to the internal surface of the vortex chamber, and an output pipe, intended for directing the fluid medium in the direction, in fact, perpendicular to the direction of the fluid medium introduction. The output pipe passes through the surface of the vortex chamber wall and projects into the internal space of the vortex chamber. In accordance with the said configuration, it is possible to reduce the loss of kinetic energy of the vortex flow of the fluid medium by the isolation of the introduced fluid medium trajectory.
EFFECT: invention provides obtaining the microbubble generator, in which the size of the formed microbubbles can be reduced, that provides effective formation of the bubbles with a nanometric range size.
5 cl, 5 dwg
SUBSTANCE: light-emitting diode (LED) comprises a base, a light-emitting structure, a first electrode and a second electrode. An U-shaped electroconductive suspension for the light-emitting structure, which is transparent for the emitted light, is made on the base. The suspension lies on the base with one arm and is rigidly connected to the base. There is a series of elements rigidly connected to the arms between the arms in the direction from the base. The elements comprise an insulating layer, a first electrode, a layer which acts a mirror and a heatsink and a light-emitting structure. The LED is made as follows. A multilayer film element is formed on the base. The materials used are such that the layer geometry and intrinsic mechanical stress thereof enable to obtain a light-emitting structure and U-shaped suspension which is electroconductive and transparent for the emitted light. The step of forming the film element includes successively making a set of layers with intrinsic mechanical stress and a set of layers of the light-emitting structure. For the latter, two areas are formed, which are arranged with a gap with a depth to the last set of layers with intrinsic mechanical stress. Areas of the film element are obtained - an area which corresponds to the arm lying on the base, an area which corresponds to the arm connected to the light-emitting structure and an area corresponding to a loop. An insulating layer, on which the first electrode is made, is formed on the area of the film element which corresponds to the arm lying on the base. A layer which acts the mirror and heatsink is formed on the area of the film element which corresponds to the arm connected to the light-emitting structure. The film element is then partially separated from the base, leaving it connected on the area which corresponds to the arm lying on the base. The set of layers with intrinsic mechanical stress is transformed under the action of the intrinsic mechanical stress into U-shaped suspension with a loop and the obtained light-emitting structure between the arms. During separation, the set of layers of the light-emitting structure with the layer which acts as a mirror and a heatsink is turned over and the latter is brought into contact with the first electrode to form a rigid connection.
EFFECT: high efficiency of converting electrical energy into light energy and heat removal, reducing the dimensions of LEDs and integration with other optoelectronic devices on a single base.
21 cl, 6 dwg
SUBSTANCE: invention refers to immunology. Presented are anti-Dickkopf 1 (anti-Dkk-1) antibodies and their functional fragments specified among the antibodies: 1) containing CDR1 VH containing the amino acid sequence SSYAIS, SYAIS or GFTFSSY; CDR2 VH containing the amino acid sequence SVSGTGLGFGTYYPDSVKG or SVSGTGLGFGTY; and CDR3 VH, containing the amino acid sequence TSLENYAFDY or SLENYAFDY; and CDR1 VL containing the amino acid sequence RASESVDDFGISFIN; CDR2 VL containing the amino acid sequence AGSKQGS; and CDR3 VL containing the amino acid sequence QQLKEVPPT; and 2) the antibodies disclosed in Table 4 presented in the application materials. Described are: nucleic acids coding the above antibodies or their functional fragments; expression vectors containing the above nucleic acids; and cells used for expression of the above antibodies or their functional fragments and containing the above expression vectors. Presented is a method for producing the antibody or its functional fragment involving the stage of culturing the above expression cell. Disclosed is a composition possessing Dkk-1 binding activity, containing the antibody or its functional fragment in a therapeutically effective amount and a pharmaceutically acceptable excipient, thinner or carrier.
EFFECT: invention enables extending the range of products for treating the diseases associated with Dkk-1 and LRP5/6 excessive reaction, which cause Wnt activation.
14 cl, 14 dwg, 14 tbl, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of organic chemistry, namely to novel derivatives of pyrazole pyridine of formula , as well as to its tautomers, geometrical isomers, enantiomers, diastereomers, racemates and pharmaceutically acceptable salts, where G1 represents H; G2 represents -CHR1R2; R1 and R2 independently on each other are selected from H; C1C6-alkoxy-C1C6-alkyl; C1-C6-alkyl; optionally substituted phenyl; optionally substituted phenyl-C1-C6-alkyl; optionally substituted morpholine-C1-C6-alkyl; or -CHR1R2 together form a ring, selected from an optionally substituted C3-C8-cycloalkyl and substituted piperidine; G3 is selected from an optionally substituted C1C6-alkoxy -C1-C6-alkyl; C1-C6-alkyl; substituted phenyl; substituted phenyl-C1C6-alkyl; G4 is selected from a substituted acyl-C1C6-alkyl, where acyl represents a group -CO-R and R stands for H or morpholine; optionally substituted C1-C6-alkyl; optionally substituted phenyl or indene; substituted phenyl-C1-C6-alkyl; optionally substituted pyridine- or furanyl-C1C6-alkyl; morpholine- or piperidine-C1-C6-alkyl; G5 represents H; where the term "substituted" stands for the groups, substituted with 1 to 5 substituents, selected from the group, which includes a "C1-C6-alkyl," "morpholine", "C1-C6-alkylphenyl", "di-C1-C6-alkylamino", "acylamino", which stands for the group NRCOR", where R represents H and R" represents a C1-C6-alkyl, "phenyl", "fluorine-substituted phenyl", "C1-C6-alkoxy", "C1-C6-alkoxycarbonyl", "halogen". The invention also relates to a pharmaceutical composition based on the formula (I) compound and particular compounds.
EFFECT: obtained are the novel derivatives of pyrasole pyridine, useful for the treatment and/or prevention of disorders or states, associated with NADPH-oxidase.
12 cl, 3 tbl, 21 ex
SUBSTANCE: orthopaedic unload is accompanied with prescribing a therapeutic diet promoting weight reduction and adequate feeding of calcium and vitamins C, D, E, K.That is combined with the oral administration of Fosamax® tablets 70 mg once a week for 6 months, Arthrodarin® capsules 50 mg in the morning and evening for 4-6 months and nasal sprayings of Miacalcic® 200 International Units/day in cycles of 2 weeks every 2 weeks for 4-6 months. What is also used is Milgamma® solution for intramuscular injections 2 ml a day, at least 10 times, with a pause of 1 day. Alflutop® 2 ml a day is injected intramuscularly daily within at least 20 days. That is combined with a therapeutic course including 8-10 therapeutic sessions each of which consists of the two stages of procedures following each other. The first of this stages involve the successive one-day abdominal decompression of the lower body from the waist down, pneumocompression massage of legs, manual and vacuum massages. The exposure segments are: hip joints - lateral surface of hips, gluteal region and paravertebral area L2-L5, cervical segment - paravertebral area C7-T1 and sacral bone. A preferred pause between the above procedures is no more than 10-15 minutes. Thereafter, the first stage of the therapeutic session is completed by conducting a hirudotherapy with the use of no more than 8 medicinal leeches per one session to be placed on the cervical paravertebral area C7-T1 and lumbar paravertebral area L2-L5, sacral bone, tail bone, abdomen within the liver, lower abdomen, painful hip segments and/or segments discoloured after the massage. Each therapeutic session is completed by successive procedures of the second stage with a pause of at least 3 days. A magnetic therapy and a barolasertherapy cover the segments: hip joints - lateral surface of hips, gluteal region and paravertebral areas L2-L5. A phonophoresis with the used of karipain gel covers the gluteal, knee and hip segments. Thereafter, 24 hours later the therapeutic session is repeated depending on the patient's state no more than 10 times. That is followed by doing therapeutic physical exercises in the swimming pool within at least 10 days. Kartalax® peptide preparation 1-2 ml in the concentration of at least 100 mcg in 1 ml diluted together with Tymalin® 10 mg is administered daily once a day for 10 days. After the injections are completed, peptide Vezugen® capsules 0.2 g are orally administered in the morning and in the evening 10-15 minutes before meals for 30 days. After the continuous and single course of Fosamax® and nasal sprayings of Miacalcic® are completed, 1-2 months later Osteogenon® tablets 830 mg are administered daily in a dose of 2-4 tablets 2 times a day, and Arthrodarin® capsules are also taken in a dose of 50 mg in the morning and in the evening for 4-6 months. The oral administration of the last-mentioned two preparations is repeated at least three months later.
EFFECT: preventing bone absorption and loss, restoring the shape, volume and normal consistence of an intraarticular cartilage that makes it possible to avoid the further progression of the disease and a necessity of surgical management in this group of patients.
3 cl, 3 ex
SUBSTANCE: invention refers to medicine, namely to traumatology and orthopaedics, and can be used for treating proximal humeral injuries. That is ensured by three-staged complex therapeutic actions. At the first stage, setting of fracture and reduction of humeral head dislocation is followed by immobilising an extremity by continuous twenty-four hour brace fixation of the proximal humerus with Desault's bandage for the period of 4 weeks. From the first therapeutic day, the patient does daily 30-minute therapeutic exercises, including isometric, static and ideomotor exercises to strengthen his/her arm muscles and to improve the circulation. That is combined with a complex reparative drug therapy. At the first stage, the anti-inflammatory preparation Arthrofoon is administered orally, while vasodilators improving nicotinic acid, trental or complamin microcirculation are injected intramuscularly daily for 10 days. The enzymatic preparations Wobenzyme or Flogenzyme are also administered in a dose of 3 tablets three times a day for 3-4 weeks. The second stage starting two weeks after the beginning of the treatment involves electric stimulation (ES) by exposing the collar and shoulder muscles from the involved side for 30 minutes to electric signals generated by an electric myostimulation device. The ES procedure requires the patient to perform 15-minute active motions by a healthy arm, and for the following 15 minutes the patient is expected to tense and relax alternatively the muscles from involved side. The therapeutic exercises are also done. The drug treatment regimen of the second stage implies administering the preparations Calcemin or Calcemin Advance for six months. That is combined with 10 daily intramuscular injections of the preparation Milgamma 2 ml. At the third stage 4 weeks after the beginning of the treatment, control X-ray imaging is followed by removing the brace. Accompanied by the reparative drug therapy continued, the complex therapeutic actions provide local injection therapy in number of 8-10 daily procedures. The biologically active reflex areas nearby the involved joint are pre-exposed to focused red laser light, and the mixed preparations Alflutop, or other chondroprotector, vitamin B12, Contrykal or Lidase, Lidocaine are injected in the same areas. Two weeks after the brace has been taken off, the patient keeps doing the therapeutic exercises twice a week continuously. The drug therapy and local injections are repeated six months later. The brace is further required for the following year if the patient is supposed to bear occupational or sports physical loads.
EFFECT: method provides faster recovery of the extremity functionality, prevents posttraumatic degenerative process in the humeral joint, recurred dislocation formation, humeral instability and contractures by optimising the humeral para-articular tissue health, improving the quality of bone tissues, first of all, of the subchondral plate and humeral head.
2 cl, 1 dwg, 2 ex
SUBSTANCE: invention relates to medicine, namely to traumatology of the maxillofacial area, and can be applied for the treatment of mandibular fractures. For this purpose the reposition and fixation of bone fragments by means of osteosynthesis or dental splints are carried out. After the reposition and fixation of bone fragments, diluted with physiological solution or the local anaesthetic botulinum toxin A - Botox or B Myoblock is introduced once into the muscles, participating in the displacement of mandible fragments. The preparation is introduced with the distribution of the total volume and maximally allowed quantity of units by 20% in the area of muscle fixation to the bones and by 80% into the zone of the highest electric activity of the muscles, verified by the method of electromyography.
EFFECT: method provides an increased rigidity of fixation of the mandible bone fragments due to the reduction of a risk of displacement and mobility of the fragments, acceleration of consolidation processes.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology and immunology. What is described is a pharmaceutical composition used for treating and/or preventing pathological bone metabolism and containing this antibody. The invention can be used in medicine.
EFFECT: antibody and its functional fragment specifically recognising human Siglec-15 and possessing the osteoclast inhibitory activity are described.
73 cl, 57 dwg, 4 tbl, 33 ex
SUBSTANCE: invention refers to medicine, namely to surgery, and can be used for treating sternomediastinitis. That is ensured by introducing a therapeutic mixture prepared ex tempore containing a broad-spectrum antibiotic tropic to bone tissue in a half a compendially recommended average therapeutic daily dose, 1 ml of lidase 32 thousand units, 1 ml of a lincomycine solution in a dose of 0.3 g, 1 ml of ketorolac tromethamine in a dose of 0.03 g, 1 ml of dexamethasone solution in a dose of 0.004 g, 1 ml of a 10% lidocaine solution and 5 ml of a 40% glucose solution. The therapeutic mixture is administered into interspinous ligaments of the spinal column at Th2-Th3, Th3-Th4, Th4-Th5, at a depth of 1.5-2 cm in a dose of 3 ml into each injection point into a patient lying on his/her side with bringing the knees to the stomach and bending the head as forward as possible. The length of treatment makes 12-14 days with the first 3 injections performed daily; the residual injections - every second day, 8-9 injections in total.
EFFECT: invention provides reducing a dose of the administered preparations and a rate of administration as compared to the systemic antibacterial therapy by an ability of the administered mixture to be accumulated in the inflammation centre, providing the therapeutic concentration with the total dose of the administered preparations reduced as compared to the systemic antibiotic therapy.
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to treating arthropathies, such as arthrosis and inflammatory loss of cartilage, tendon disorders and/or degenerative spine diseases. What is presented is a pharmaceutical composition for the above application, containing a corticosteroid and a cytokine antagonist - a natural or recombinant protein of interleukin IL-1Ra antagonist, particularly orthokine or anakinra, and optionally a growth factor; the composition is injectable into an injured nerve root, or into an injured intervertebral disk, or into their local context, or for intraarticular injection. There are presented: a kit comprising the pharmaceutical composition with the above cytokine antagonist and optionally the growth factor, and the pharmaceutical composition with the corticosteroid; using the above cytokine antagonist and optionally the growth factor for preparing the pharmaceutical composition to be used in combination therapy together with the corticosteroid for the above application; using the corticosteroid for preparing the pharmaceutical composition to be used in combination therapy with the above cytokine antagonist and optionally the growth factor for treating the above arthropathies, tendon disorders and/or degenerative spine diseases.
EFFECT: clinical success of treatment manifested by apparent joint detumescence, pain reduction by 60-100%, functional improvement of the joint, with the effect persisting 8 months later and more after the treatment.
39 cl, 1 tbl
SUBSTANCE: invention refers to medical and veterinary traumatology, surgery and concerns treating various bone injuries, particularly fractures and fissured fractures. That is ensured by fixing injured bone fragments with a plaster splint or a polymer bandage. An aqueous solution containing 1-hydroxyethylene diphosphonic aicd in an amount of 1.80-2.06 g/l, anhydrous calcium chloride in an amount of 1.44-2.22 g/l, gadolinium (III) nitrate hexahydrate in an amount of 0.30-0.40 g/l, dysprosium (III) chloride hexahydrate in an amount of 0.038-0.076 g/l is introduced into the fracture; pH of the solution is 7.3-7.8. Before administering into the fracture, the above agent is heated up to 30-100°C, kept at this temperature for 1-48 hours, and cooled to room temperature.
EFFECT: method provides reducing the osteoanagenesis time within the injury, including by providing the optimal thermodynamic crystalline maturing with low toxicity of the solution and its storage stability.
3 tbl, 35 ex
SUBSTANCE: what is applied is a stocking coating of an autogenous bone with a patient's platelet-rich plasma. Bone marrow aspirate from the patient's ilium and/or mesenchymal stromal cell autoculture prepared of the aspirate by culturing in vitro are injected under the coating layer into the autogenous bones. The autogenous bones are placed tightly in the bone defect to cover the defect area with adjacent soft tissues.
EFFECT: complete and effective synthesis of the bone tissue continuity by creating conditions of proliferative process isolation and osteoresorption process deceleration in the autogenous bone with no undesired immunological responses in the plasty area.
1 dwg, 1 ex
SUBSTANCE: invention represents an agent for treating a pulp inflammation differing by the fact that it contains Bezornil ointment and Dycal ivory paste in ratio 1:1 blended until smooth.
EFFECT: invention provides relieving oedema and pain on the day of doctor's appointment, accelerating the pulp tissue regeneration process, creating the reparative dentin within 30 days, and ensures the higher clinical effectiveness in the pulp inflammations.
2 ex, 1 tbl