Method of neurosyphilis diagnostics
SUBSTANCE: method of neurosyphilis diagnostics includes the microscopic analysis of cerebrospinal fluid samples, with carrying out edge dehydration of the cerebrospinal fluid samples, and their microscopic analysis being carried out in a polarised light; in case of the detection of anisotropic structures in the form of dendrites or spherulites inside which oval-shaped formations, containing lipids, are located, an early form of neurosyphilis is diagnosed; and in case of the detection of a multitude of ovals, aggregated in the form of balls, included in the anisotropic structures and/or located separately, late meningovascular neurosyphilis is diagnosed. The invention task is to obtain objective criteria for the diagnostics of neurosyphilis, provision of early, including pre-clinical diagnostics of the disease, reduction of the terms for obtaining data, reduction of costs for carrying out analyses.
EFFECT: in a number of cases the method is the only one which makes it possible to obtain the valid criteria for diagnostics in the form of a picture of the brain structures destruction, which makes it possible to diagnose neurosiphilis confidently and prescribe specific therapy in due time.
4 dwg, 3 ex
The invention relates to medicine, namely to laboratory diagnosis, and can be used in determining the severity of lesions of brain structures pale pallidum in patients with syphilis in the anamnesis.
The problem of timely diagnosis of neurosyphilis remains relevant despite the creation of a broad base of laboratory tests, including clinical and serological indices in the study of cerebrospinal fluid. However, due to insufficient sensitivity of tests used often situations arise in which we cannot exclude or confirm the invasion of Treponema pallidum in the structure of the nervous system. In some cases the skill of the Clinician is crucial in the diagnosis of this pathology, and the timely appointment of adequate therapy helps prevent irreversible organic disorders of the nervous system and death of the patient. At the same time, there are some forms of early asymptomatic neurosyphilis, i.e. no clinical signs of disease with loss of sensitivity to traditional laboratory methods and follow.
Known method for the diagnosis of neurosyphilis in the study of cerebrospinal fluid by using serological tests (Lukyanov A. M. Neurosyphilis. Modern aspects of the clinic, diagnosis, therapy. Minsk, Paradox, 2009, pp. 246-263),�Lucasi definition and research VDRL (Veneral Disease Reserch Laboratory), TPHA, Rifts (REEF with whole spinal fluid, ELISA-IgG.
Currently, however, the results of serological tests in the study of cerebrospinal fluid in most cases, are insensitive or low-sensitive, which makes the diagnosis of neurosyphilis. Such situation has developed in the 21st century in connection with the change in the response of humans to the widespread use of antibiotics, which was not mentioned earlier (first half of the 20th century) when the above tests were highly sensitive and definitely solved the problems of diagnosis of neurosyphilis.
Known method for the diagnosis of neurosyphilis (Patent RF №2230323, IPC 33/50, publ. 2004), including the identification of sphingomyelin in the cerebrospinal fluid of patients with flow-through thin-layer chromatography, the level of which diagnosis is performed.
The disadvantage of this method is its complexity and time consuming, require expensive equipment and reagents, and the availability of highly qualified personnel. This method can be carried out only in specialized laboratories of the research Institute, so in practice it is difficult to use it.
Closest to the claimed invention is a method of diagnosis of neurosyphilis, including microscopic examination of cerebrospinal fluid (Lukyanov A. M. Neurosyphilis. Modern aspects of TC�Niki, diagnosis, therapy. Minsk, Paradox, 2009, p. 233).
The disadvantage of this method is that currently the body's sensitivity to inflammatory processes caused by the introduction of Treponema pallidum in the brain structure, decreased sharply due to the wide use by the population antibacterial drugs that have influenced a decrease in the immune response to a foreign antigen.
In connection with this object of the invention is to eliminate these drawbacks, and to provide objective criteria for the diagnosis of neurosyphilis, early, including preclinical diagnosis of the disease, reducing the time of data acquisition, reduction in the cost of conducting research.
For this method, the diagnosis of neurosyphilis, including microscopic examination of cerebrospinal fluid specimens, it is suggested to marginal dehydration of samples of cerebrospinal fluid and microscopic examination of exercise in polarized light. Thus the detection of anisotropic structures in the form of dendrites or spherulites, which are located inside of the formations in the shape of ovals, containing lipids, are diagnosed with an early form of neurosyphilis; in identifying the many ovals that are grouped in the form of balls included in the anisotropic structure and/or separately located, d�Instituut a late neurosyphilis.
It is known that the liquid crystal composition of the structures based on the "lipid - water", "lipid - protein - water", which when translated in solid state the method of boundary dehydration of biological fluids form the structure observed by microscopy in polarized light (Shabalin V. N., Shatokhina S. N. The morphology of biological fluids of man. M., Triad, 2001, p. 52-54). The cell membrane of the brain and spinal cord is rich in lipids and proteins. When the destruction of degradation products into the cerebrospinal fluid, one function of which is to eliminate toxins from the brain tissue. As Treponema pallidum penetrates into the brain and forms in it multiple foci of destruction, causing pathological changes in the Central nervous system (paresis, paralysis, stroke, mental disorders, etc.), the idea is to explore patterns of cerebrospinal fluid systems "lipid - water", "lipid - protein - water" in patients who have had syphilis, for the purpose of diagnosis of destructive processes in the brain tissue.
Fig. 1 shows the spherulite cerebrospinal fluid of a healthy person. It is seen that the structure is monolithic and lacks any additional inclusions (×300); Fig. 2 shows the anisotropic dendrite with the presence of inclusions in the form of ovals containing the lipids in ill�with early asymptomatic neurosyphilis. Amorphous ovals characterize the processes of destruction in the Central nervous system (×300); Fig. 3 - lots of ovals, grouped in the form of balls located inside the spherulite and outside (×50) of Fig. 4 - the same (×200). The method is as follows.
The patient, a history which was syphilis (seropositive reaction serum for syphilis), receive cerebrospinal fluid for diagnosis of neurosyphilis. To do this, conduct a study on the method of boundary dehydration, which on the surface of a glass slide put a drop of cerebrospinal fluid and cover it with a cover glass (creation analysis cell). After 72 hours the cells mikroskopiruût in polarized light at various magnifications and the detection of structures is diagnosed early or late form of neurosyphilis.
Patient M., 36 years old. 2 years ago diagnosed with syphilis. Received a course of specific therapy. Currently the patient is in a psychiatric hospital, inadequate, disoriented in space. The results of clinical studies of the cerebrospinal fluid: the concentration of protein - 0,47; cytosis 5/3 (lymphocytes - 3, monocytes - 2), which corresponds to the parameters of normal.
Serology: VDRL - negative, TPHA - weakly positive (2+), Rift - positive (3+), ELISA G - negative�, i.e. the outcome is uncertain.
The study results of the claimed method: detected dendrites in their structure ovals (Fig. 2).
Conclusions: neurosyphilis, early form.
The examining Clinician: there are medical history and neuropsychological data, which together with the results obtained by the claimed method, allow the diagnosis of neurosyphilis and prescribe a specific course of therapy for neurosyphilis.
It was followed by adequate treatment, after 6 months re-examination of the cerebrospinal fluid by the proposed method did not reveal structures characteristic of neurosyphilis.
Patient F., 52 years. Installed late syphilis. A course of specific therapy not received. Currently enrolled in the Department of neurology at the effects of cerebral infarction, right-sided hemiparesis.
The results of clinical studies of the cerebrospinal fluid: the concentration of protein - 0,21; cytosis 8/3 (lymphocytes - 8), which corresponds to the parameters of normal.
Serology: VDRL - negative, TPHA - weakly positive (2+), Rift - weakly positive (2+), ELISA G is weakly positive (R=2,5), i.e. the outcome is uncertain.
The study results of the claimed method: the analytical cell was found a large number of ovals that are grouped�'s in the form of balls, included in anisotropic structures and outside them (Fig. 3, 4). Conclusions: neurosyphilis, late meningovascular form.
The examining Clinician: there is neurological data together with the results obtained by the claimed method, allow the diagnosis of late forms of neurosyphilis and prescribe a specific course of therapy for neurosyphilis.
Patient O., 40 years. Moved syphilis 8 years ago. Received 2 courses of specific therapy.
The study carried out by the above procedure.
The study results of the claimed method: all the analytical cells detected spherulites with the norm (Fig. 1).
Conclusion: signs of neurosyphilis are missing.
The examining Clinician: clinical signs of neurosyphilis is not found.
The proposed method studies have been conducted among 106 people, with a history of syphilis and various neurological symptoms, was obtained cerebrospinal fluid for diagnosis of neurosyphilis.
Studies have been carried out by known methods: neurosyphilis was confirmed by serological studies and the results of the cell count of the cerebrospinal fluid in 42 people. The remaining 64 patients were obtained negative or weakly positive results.
Those patients (64 men), which demanded�camping further diagnosis, a study was conducted of the claimed method. The results showed that the destructive process in the Central nervous system caused pallidum pale, had only 26 people. These patients were assigned to additional treatment and at the follow-up study after 6 months was noted positive dynamics of neurological symptoms (recovery of speech, sensation, motor function) and no signs of neurosyphilis claimed method when you study. The remaining 38 patients, the diagnosis of neurosyphilis was withdrawn.
The claimed method for the diagnosis of neurosyphilis in some cases is the only one that allows to obtain reasonable diagnostic criteria in the form of a picture of the destruction of the structures of the brain that allows you to confidently make the diagnosis of neurosyphilis and promptly appoint a specific therapy. In sbeh MOCVD this method demanded by clinicians, especially in those cases where the diagnosis of neurosyphilis is controversial. The modern diagnostics provides targeted and therefore effective therapy.
Method for the diagnosis of neurosyphilis, including microscopic examination of samples of cerebrospinal fluid, characterized in that the conducting boundary dehydration of the samples spinnomozgova� fluid, and their microscopic examination is carried out in polarized light and the detection of anisotropic structures in the form of dendrites or spherulites, which are located inside of the formations in the shape of ovals, containing lipids, are diagnosed with an early form of neurosyphilis, and in identifying the many ovals that are grouped in the form of balls included in the anisotropic structure and/or separately located, are diagnosed with a late neurosyphilis.
SUBSTANCE: group of inventions relates to medicine, cosmetology, production of food products, vitamins, food supplements, drugs and describes versions of device for realisation of non-invasive potentiometric determination of oxidant/antioxidant activity of biological tissues, which includes device for measuring potentials and double-sided electrode, made in form of plate with similar working surface, covered with electricity-conducting gel, containing mediator system. Electrodes are fixed on biological tissue in such a way that one working surface, playing role of measuring electrode, is in direct contact with biological tissue via gel, second working surface pale role of comparison electrode. Electrodes contact with each other via gel, with oxidant/antioxidant activity being determined by formulae with application of difference between final and initial potentials.
EFFECT: simplification, as well as increase of accuracy and reliability of determination, is achieved.
14 cl, 3 tbl, 4 dwg
SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.
EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.
2 tbl, 3 dwg, 3 ex
SUBSTANCE: test tray comprises a case 1 made of an optically transparent material. From one end face, the case 1 has a stop plug 2 with a hole 3, a connecting pipe 4 threaded to connect to either a haemofilter 5, or a cap 7. The case 1 comprises a movable piston 8 connected by a rod 10 to a handle 11. Electrically supplied electrodes 12 are arranged on surfaces of the stop plug 2, piston 8 and on the inner surface of the case 1. The electrodes 12 are connected to contact groups of a device - a laser analyser - through conductors 13, 14. What is disclosed is an alternative version of the structural embodiment of the test tray.
EFFECT: sterile measurement of the colloidal fluid sample.
17 cl, 5 dwg
SUBSTANCE: technique involves the three-stage diagnosis of all the patients suffering tumour diseases of various localisations. The first stage involves CT-densitometry performed every 6 months; if the CT-densitometry shows sites with varying spine bone density by 30% and more, the second diagnostic stage that involves a transpedicular biopsy is initiated. If the biopsy material appears to contain no tumour material, the third diagnostic stage starts with F-18 positron-emission tomography (PET-CT).
EFFECT: improving the early diagnosis of the spinal tumours.
SUBSTANCE: clinical assessment of the oral mucosa state is ensured by examining non-stimulated oral fluid or swabs. That involves determining five parameters: content of yeast-like fungi Candida in the yeast or mycelia form (1), concentration of secretory immunoglobulin A (SIgA) (2) and lysozyme (3), emission light sum (S) 5 minutes before chemoluminescent examination (4); the Wood-beam beam luminescent examination covers marginal portions of the gum and apexes of interdental papillas, buccal mucosa within dental occlusion, dorsal surface of the tongue within the thread-like processes, and performing morphological examination of the multilayer squamous epithelium of the buccal mucosa along the dental occlusion line (5). The derived results enable diagnosing the absence of pathogenic microflora and oral mucosa pathology, Candida carriage or chronic oral candidiasis in the mycelia or yeast form in the form of hyperkeratosis or leukokeratosis.
EFFECT: using the invention enables increasing the differential diagnostic accuracy for keratolytic processes in the form of white manifestations.
10 dwg, 5 ex
SUBSTANCE: method includes selection, and crushing of biomaterial, two-stage extraction of pesticides with n-hexane, purification of biomaterial from coextractive substances with concentrated sulphuric acid, formation of concentrate of n-hexane extract of pesticides, drying, sample formation by dissolution in 0.5-1 ml of n-hexane and carrying out gaschromatographic identification.
EFFECT: invention is characterised by higher effectiveness and accuracy of research and can be used in biology, ecology, medicine for gaschromatographic identification of organochlorine pesticides, namely α-HCCH, β-HCCH, γ-HCCH, DDT, DDD, DDE, in various biomaterials, such as lipids of internal organs and tissues, blood, milk, bird feathers.
2 cl, 1 dwg
SUBSTANCE: invention represents a method for preclinical study of cardiotropic antiarrhythmic drugs, involving determining the bioelectric parameters in isolated multicellular perfused preparations and measuring an action potential duration, differing by the fact that the isolated multicellular perfused preparations are presented by rat's pulmonary vein myocardium; the parameters are measured in three operation modes of the multicellular preparations; a resting potential is additionally measured; varying APD 90%, related APD 50%/APD 90%, a spontaneous shear velocity of the resting potential, the most positive membrane potential in the resting preparation, a spontaneous activity train repetition rate, spontaneous action potential train repetition and variability frequency, post-depolarisation number and intensity, as well as a shear membrane potential corresponding to the beginning of train activity are used to evaluate the signs of antiarrhythmic and arrhythmogenic action.
EFFECT: more reliable prediction of the antiarrhythmic action of the potential pharmacological agents and reduction of experimental phase time.
FIELD: measurement equipment.
SUBSTANCE: invention comprises a method of determination of nano trace contaminants, supposing the use of emulsion from liquid crystal drops, dispersed in water and capable to change a configuration of liquid crystal drops in presence in the emulsion composition of foreign admixtures, measurement of change of intensity of light scattered by the emulsion, according to which it is possible to evaluate the content and density of required admixtures differing by that for a liquid crystal the compounds capable to trans-cis-transition under the effect of actinic light are selected and before measurement of change of light intensity the emulsion is illuminated additionally by actinic light, thus ensuring change of configuration in liquid crystal drops due to trans-cis-transition in liquid crystal molecules.
EFFECT: increase of sensitivity of method of determination of nano-trace contaminants.
SUBSTANCE: invention refers to a method for identifying living and dead mesozooplankton in seawater samples, which involves taking samples, staining the organisms with suitable colouring material, giving a visual estimation of the colour intensity of the units under the microscope, which is combined with microphotographying the units with an adjustable camera without changing the settings keeping throughout a photographic session of at least one sample; thereafter colour and brightness specifications average for each unit are measured in the formed images with using a painting program, e.g. Adobe Photoshop package, and the units are referred to living or dead by a discriminative analysis of the varied digital values.
EFFECT: improving the method.
SUBSTANCE: identification method of blue pus bacillus Pseudomonas aeruginosa involves inoculation of the test material on a hard substrate, incubation of the inoculum under anaerobic conditions at the temperature of 37°C during 16-18 hours. The obtained bacterial mass in the amount of one bacteriological loop is placed in 300 mcl of a physiological solution and warmed-up at the temperature of 98-99°C during 20-30 minutes and centrifuged at 12000 revolutions per minute during 30 seconds. To a supernatant there added is colouring material for electrophoretic detection in the quantity of 0.5 mcl and 20 mcl is added to a well with a size of 4×1 mm 1.2% agarose gel on TAE-buffer with 10 mcl of 1% ethidium bromide. Besides, the amount of 3 mcl of the solution of standard DNA-marker 1 kb containing DNA fragments in the range of 250-10000 bp is added to the test well. Horizontal electrophoresis is performed during 15-20 minutes, and at detection on the obtained electrophoregramme of the test bacterial mass of three strips radiating in ultraviolet light, one of which corresponds to fragments of standard DNA-marker with the size of 10000 bp, the second one corresponds to fragments of standard DNA-marker with the size of 6000-8000 bp and the third strip at the end of the track in the form of a whisk, which corresponds to fragments of standard DNA-marker with the size of less than 750 bp, there identified is Pseudomonas aeruginosa in the test bacterial mass.
EFFECT: method allows quick and complete identification of pigment-shaping and non-pigment strains Pseudomonas aeruginosa in a test bacterial mass.
5 dwg, 2 ex
SUBSTANCE: method involves examining blood erythrocytes. Blood erythrocyte suspension fluorescence intensity is measured at wavelength of 500 nm polarized along light axis and then at wavelength of 493 nm in perpendicular to the light axis for each polarization direction with following anisotropy value being calculated. Its deviation from norm proves availability of hemolytic disease.
EFFECT: accelerated examination process; reduced risk of traumatic injuries of fetus or newborn.
FIELD: veterinary medicine.
SUBSTANCE: method involves carrying out blood erythrocyte micro electrophoresis in alternating electric field with sign change frequency equal to 0.3-1 Hz. Electric field having current intensity equal to 4.5-5.0 mA is applied. Treatment is applied at 36-38°C. Oscillation amplitude being equal to 3 mm and less, endotoxicosis is detected.
EFFECT: high accuracy and simplicity of the method.
SUBSTANCE: method involves applying integrated diagnostics approach with blood serum examination being applied using infrared spectrometry methods. Sample absorption spectra in the area of 1200-1000 cm-1 are recorded, absorption peak heights having maximum at 1170, 1165, 1140, 1125, 110, 1070, 1025 cm-1 points are measured. Then, mean peak height C and ratio X of peak height at 1125 cm-1 point of maximum to C are calculated. X≥0.7 being the case, light myocardium injury degree is to be diagnosed. 0.7>X≥0.53 being the case, moderate severity degree case is to be diagnosed. X<0.53 indicates grave injury to take place.
EFFECT: high reliability of diagnosis.
FIELD: analytical methods in medicine.
SUBSTANCE: cells of tested organs are subjected to alternating cytological electrophoresis. When cell vibration amplitude rises by at least 20%, drug is regarded to be appropriate for treatment.
EFFECT: increased drug selection efficiency.
FIELD: medicine, hepatology.
SUBSTANCE: the present innovation deals with detecting bilirubin content both before and after curative starvation, moreover, curative starvation one should introduce antipyrine at the dosage of 1 mg/kg, in a day it is necessary to detect antipyrine content in saliva and urine and bilirubin content and at bilirubin content being 30-40 mM/l one should starve at daily intake of 400-450 kcal/d for 3 d, then one should again detect bilirubin level and at its increase by 50-100% one should again introduce antipyrine at the dosage of 1 mg/kg and according to its delayed half-life by 20-50% it is possible to diagnose Gilbert's disease.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, otorhinolaryngology.
SUBSTANCE: the present innovation deals with treating diseases of the upper respiratory tract. One should detect disorders of mucociliary transport (MCT) due to measuring the motor parameters of ciliated epithelium cilia, at least, the rate of mucociliary transport that includes material sampling for further testing as ciliary epithelial cells and registration of cilia fluctuations in human respiratory tract due to TV microscopy performed in patient's lifetime. Material for testing should be sampled from mucosal surface of respiratory tract, and therapeutic tactics should be chosen depending upon motor velocity of cilium's tip obtained based upon mathematical modeling followed by calculations by the formula: , where Vc - velocity of cilium's tip [mcm*sec1]; C1, C2, α - calculated as average values being characteristic for every of human body states under investigation: healthy persons, patients with either acute or chronic forms of sinusitis by the developed model of cilia movement; C1 - curvature of cilium's working length, constant being equal to 1/38 mcm-1; C2 - cilium's curvature from its working length up to the tip [mcm-1]; α - cilium's inclination angle against vertical position [degrees]; π = 3.1417 - constant; L, Ts and Te - parameters measured individually in every patient; L - cilium's length [mcm]; Ts - time from the onset of movement till maximal straightening of cilium's tip [sec]; Te - time of efficient impact [sec]. Moreover, the value being Vc = 9.6 - 11.3 mm/min corresponds to normal MCT functioning. For patients with chronic purulent sinusitis beyond exacerbation accompanied with MCT disorder it is necessary to prescribe complex therapy: surgical or conservative therapy with preparations of secretolytic and secretomotory action. For patients with chronic purulent sinusitis beyond exacerbation without any MCT disorder one should prescribe surgical therapy. All patients with acute sinusitis with MCT disorder should be prescribed complex conservative therapy with preparations of secretolytic and secretomotory action. The method enables to specify optimal therapy based upon scientifically proved indications of investigations by taking into account individual peculiarities of every patient.
EFFECT: higher efficiency.
5 dwg, 3 ex, 2 tbl
FIELD: medical engineering.
SUBSTANCE: device has non-absorbing substrate having hydrophilic target region, which is covered with reagent by applying non-impact micro-drop printing method to produce practically uniform reagent layer. The device is in particular usable for measuring blood coagulation time. Preferential invention embodiment involves determining blood coagulation time by carrying out monitoring of light transition through the target region as the blood sample cover interacts with the reagent.
EFFECT: high reliability of analysis results.
20 cl, 9 dwg
SUBSTANCE: one should register the values of pH and Redox potential (Red) of liquid means in bio-objects simultaneously. It is necessary to plot a graph ▵pH/▵Red according to fluctuations of variation values in measured parameters at their temporal diagram to transfer it into a columnar one where the height of every column is proportional to the area of this graph between neighboring measurements, correspondingly. According to the ratio of area sums of positive columns to that of negative ones during a certain period of time ( from a minute- to a year-long ones) one should conclude upon a state in a bio-object. The value ranged 0.5-2 is considered to be a standard. The method enables to provide efficient control for a two-phase systemic process.
EFFECT: higher efficiency and accuracy of diagnostics.
1 cl, 9 dwg, 5 ex, 1 tbl
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should perform crystallographic study if cerebrospinal fluid and at protein concentration in liquor samples being from 0.50 g/l and higher at predominance of crystals as suppressed dendrites upon a crystallographic picture one should diagnose a malignant cerebral tumor, and at protein concentration being below 0.50 g/l and predominance of crystals as branched dendrites - a benign cerebral tumor. The innovation enables to fulfill not only due diagnostics of cerebral neoplasms, but detect the degree of tumor process malignancy at the early stages of its development.
EFFECT: higher accuracy of differential diagnostics.
3 dwg, 2 ex, 1 tbl
FIELD: biology, medicine, in particular histological investigation of shells with natural surface by using light-optical microscope.
SUBSTANCE: claimed method includes surface relief investigation by using plane field microscope in reflected light at one-side falling shadow-forming lighting. Light-reflecting ability of specimen surface is provided by silver impregnation. Said impregnation is carried out by specimen hold in 10 % silver nitrogen solution for 10 min followed by reducing thereof with 1 % ascorbic acid solution for 1 min.
EFFECT: method for investigation of natural shell surface relief of improved quality due to visual representation of surface three-dimension image on tissue level.
3 cl, 4 dwg