Method of obtaining 7-hydroxyroyleanon, possessing antimicrobial activity
SUBSTANCE: invention relates to method of obtaining 7-hydroxyroyleanon, possessing antimicrobial action. said method includes extraction of crushed roots of salvia officinalis with 96% ethyl alcohol with further extract evaporation, processing with water, alcohol distillation and processing with hydrophobic solvent or extraction of said raw material with chloroform with further extract processing with water and evaporation; after which target product is extracted from organic phase by transfer into water-soluble phenolates, with processing with sodium hydroxide water solution; alkali solution is washed with chloroform; acidified with hydrochloric or sulphuric acid; obtained sediment is filtered; dried and crushed.
EFFECT: invention is characterised by improved process manufacturability and provides obtaining individual substance with higher antimicrobial activity than previously extracted royleanon derivatives from salvia officinalis.
2 tbl, 6 ex
The invention relates to medicine, namely to chemical-pharmaceutical industry and relates to a method of obtaining antimicrobial agents: 7-hydroxytoluene.
The literature describes methods for isolating releasenew different from 7-hydroxytoluene and his acetoxypropionyl the structure and location of the substituents, from different raw materials:
extraction with diethyl ether (Coleus fredericii, leaves) with subsequent column chromatography ;
extraction with diethyl ether (Coleus coerulescens, grass) followed by fractionation in a solvent system diethyl ether-acetone 1:1 and petroleum ether (30-60°) - methanol 1:9 and separated by column chromatography on silica gel ;
extraction with diethyl ether (Plectranthus barbathus, leaves, Plectranthus sanguinens, leaves), by evaporation of the solvent and chromatographytandem on Sephadex LH-20 ;
- extraction successively with hexane and diethyl ether (Plectranthus edulis, leaves), by evaporation of the solvent and chromatographytandem on Sephadex LH-20 ;
- 7-acetoxysilane get from the roots of Inula roylana by extraction with methanol, concentration of the extract, from which stands out the residue the target product .
In all these examples aim to highlight, identify, or determine the structure of substances, where the completeness of extraction is not the main task, therefore extractants and processes �e are optimal for industrial technology.
A method of producing the amount of releasenew  with antimicrobial activity from the roots of Salvia officinalis, including the extraction of raw materials chloroform, filter, evaporate the chloroform extract, followed by chromatographic separation in a thin layer of sorbent and elution adsorption zone of releasenew.
This method is good at solving analytical task of determining the amount of Raileanu, but unacceptable in technology, especially large-scale, due to the presence of thin layer chromatography, eluent a large number and complexity of the process.
The aim of the invention is to provide a method of obtaining as individual drug compounds with the predominant antimicrobial activity, 7-hydroxytoluene, development of a method of purification of the target product from the impurities of the lipid nature of the reduction of the time and good reproducibility. Antimicrobial activity of 7-acetoxysilane against Golden staph is 62.5 μg/ml, 7-hydroxytoluene - 15.6 ág/ml (Table. 2).
This object is achieved in that the extractant used 96% ethanol. After distilling off the solvent to ~2% of the original volume of the distillation residue is diluted with water, completely distilled off the alcohol and treated with chloroform, followed by extraction with a�new product from chloroform with an aqueous solution of alkali, washed with an alkaline solution with chloroform, precipitated with hydrochloric or sulfuric acid, the precipitate is separated, washed with water, dried. Extraction of the target product with an alkali in the form of a phenolate, soluble in water, solved two problems at once - the less active hydrolysis of 7-acetoxysilane and separation from 7-hydroxytoluene of lipids remaining in the chloroform phase. Washing the alkaline solution of the desired product with chloroform removes lipids entering the aqueous alkaline phase with insufficient separation of organic and aqueous alkaline phase. The extraction of raw materials can be carried out directly chlorinated hydrocarbon (chloroform) followed by carrying out the process in this way. Use head on stage as the extractant ethanol, followed by evaporation and dissolution of extractives in chloroform for reasons of environmental safety.
The proposed method the criterion of "novelty" is that:
- Department of releasenew from a mixture of lipids is carried out by treating a chloroform extract of the aqueous alkali, in which oleanane transferred in the form of a phenolate,
- 7-acetoxysilane hydrolyzed to 7-hydroxytoluene, and lipids remain in chloroform,
- the final product is precipitated from alkali acid that is breitenlohner, than fill the column on the sorbent. Comparative data on the physicochemical characteristics and biological activity of 7-hydroxytoluene - drug and 7-hydroxytoluene - standard sample show that the proposed method allows to obtain the target product with the necessary qualitative and quantitative composition and biological properties.
The method consists in the extraction of the crushed roots of Salvia officinalis 96% ethyl alcohol, the evaporation of the alcohol, adding water. Next, the aqueous phase is treated with chloroform, the chloroform solution washed with water, treated with a solution of sodium hydroxide. From alkaline extraction is removed by vacuum traces of chloroform, filtered, and acidified with hydrochloric or sulfuric acid. The precipitate of the desired product was filtered off, washed with water and dried (PL. 1).
The technical result of the proposal is to expand the use of plant material the roots of Salvia officinalis, to date representing waste when growing sage to obtain essential oils from the aerial parts, in the transformation of the sum of releasenew: 7-acetoxysilane and 7-hydroxytoluene in individual substance - 7-hydroxytoluene with the highest antimicrobial activity, improving the manufacturability of the process by replacing long p�of acessa chromatography on a sorbent precipitation with acid from the alkaline solution.
200.0 g of crushed root sage is placed in a glass flask, pour 1 liter of ethyl alcohol (1:5) and the extraction is carried out at room temperature for 16 h with stirring. The first extract is drained, pour the raw material alcohol in the merged volume of the extract and extracted as in the first case. A third extraction was carried out analogously for 16 h. the combined alcoholic extracts (about 2.3 liters) was evaporated to approximately 2% of the original volume (in the case of a continuous process third extraction served on fresh raw new download), diluted with water 3-4 times and completely distilled off the alcohol. The bottoms (150-200 ml) was treated three times with chloroform, 200, 100, 100 ml. Chloroform, the solution is extracted with 4 times 1% aqueous solution of sodium hydroxide. The ratio of water-alkaline and organic phases 0,4:1, 0,3:1, 0,3:1, 0,2:1. The combined alkaline extract was washed with chloroform (~50 ml), treated with a vacuum, filtered through cotton wool and acidified with 5% hydrochloric acid solution to pH 3. The precipitate was separated by filtration, washed with water until neutral reaction of the wash water, dried, triturated.
The yield of 0.58 g (0.3% by weight of raw materials).
The content of 7-hydroxytoluene of 53.7%.
200 g of crushed roots of Salvia placed in a glass flask, pour 1 liter of 95% ethyl alcohol (1:5) and the extraction is carried out at room temperature within t�e 4 h with stirring. The first extract is drained, pour the raw material alcohol in the merged volume of the extract and extracted for 3 h under the same conditions as the first extraction. A third extraction was carried out analogously to the second (3 hours, room temperature, mixing). The combined alcoholic extracts (~2.3 liters) was evaporated to ~2% of the original volume (in the case of a continuous process the third extract is served on fresh feedstock), followed by carrying out the process indicated in example 1.
Get 0,66 g (0,33% by weight of raw materials).
The content of 7-hydroxytoluene to 55.9%.
200 g of crushed roots of Salvia placed in a glass flask, pour 800 ml of chloroform (1:4) and the extraction is carried out three times at room temperature with stirring for 16 h. the combined chloroform extract was washed with water (~80 ml), evaporated to 340 ml and four times extracted with 2% aqueous solution of sodium hydroxide in 150 ml. combined alkaline extract was washed with chloroform (~50 ml), treated with a vacuum to remove traces of chloroform and acidified with 5% hydrochloric acid to pH 2-3. Fallen Golden yellow precipitate was filtered, washed with water until neutral, dried and milled.
Get 0,53 g (0.3% by weight of raw materials).
The content of 7-hydroxytoluene representing 54.7%.
1 kg ismalic�fucking raw materials are placed in the extractor in stainless steel vertical cylindrical with conical bottom, with the bottom release (d=200 mm h=360 mm), pour 5 liters of 95% ethyl alcohol (1:5) and the extraction is carried out at room temperature for 16 h with stirring. The first extract is drained, pour raw materials in the merged volume of the extract and extracted as in the first case. A third extraction is carried out in a similar mode. The combined alcoholic extract was evaporated to 200 ml, add 150 ml of water, evaporated to remove alcohol, bring the volume of the aqueous phase to 650 ml, extracted with chloroform 350, 250, 250 ml), the combined chloroform extract was washed with water (~150 ml) and extracted 4 times with 1% sodium hydroxide solution 300, 220, 220, 150 ml the combined alkaline extract was treated with vacuum to remove traces of chloroform, filtered through cotton, diluted with water twice, acidified with 5% sulfuric acid to pH 2. The yellow precipitate was filtered, washed with water until neutral, dried, ground.
Get 3,49 g (0,35% by weight of raw materials).
The content of 7-hydroxytoluene is 48.5%.
50.0 g of crushed roots of Salvia placed in a glass flask, pour 250 ml of ethyl alcohol (1:5) and the extraction is carried out at room temperature and with stirring for 4 hours. Receive 200 ml of the extract. A second extraction was carried out for 3 hours, volun� 200 ml of the extractant The volume of extract 195 ml Third extraction is carried out as a second for 2 hours. The volume of extractant 195 ml of the extract (200 ml). the combined extracts evaporated to ~30 ml, diluted with 120 ml of water and evaporated to remove the alcohol. Then the aqueous phase was successively extracted with methylene chloride 7×40 ml to backlash last retrieved on royleana (purple staining spots on filter paper or in a thin layer of silica gel from exposure to ammonia vapors). United chlorotoluene extract (240 ml) was treated with alkali 40 ml, 3×30 ml). the combined alkaline extract was treated with vacuum and acidified with 5% hydrochloric acid. Precipitated yellow precipitate was filtered, washed to neutral reaction and dried. Get 0,043 g (0,086% by weight of raw materials). The content of 7-hydroxytoluene 31,76%.
50.0 g of crushed roots of Salvia three times extracted as in example 5, 4, 3 and 2 hours. The ratio of the volume of the extractant and the weight of the raw material 5:1. The volume of the extractant on the 1st extraction of 250 ml for each additional equal to the merged volume of the extract, i.e., 200 and 195 ml) Then the combined extract is evaporated to remove the alcohol dichloroethane is treated with 6×40 ml. of the United dichlorethane extract in the amount of 232 ml was filtered through cotton wool and treated with 1% aqueous NaOH 50 ml,42 ml, 2×30 ml). The combined alkaline extract (151 ml) was filtered, treated with vacuum and acidified with 5% hydrochloric acid. The precipitate was filtered off and washed until neutral, dried, crushed.
Get of 0.036 g (0,072% by weight of raw materials). The content of 7-hydroxytoluene 38,63%.
The technology comparison with the prototype presented in the table. 1.
Using the proposed method in comparison with the known provides the following benefits:
- use head on the stage of production is practically non-toxic extractant - ethanol;
- lack of heating processes toxic and flammable solvents;
- conversion of the less active 7-acetoxysilane more active in 7-hydroxytoluene (62,5 µg/ml and 15.5 µg/ml, respectively);
- carrying out hydrolysis of 7-acetoxysilane and the separation of lipophilic contaminants in one operation: royleana in the form of phenolate passes into the aqueous phase and the lipophilic impurities remain in chloroform;
- target product deposition from an aqueous solution of sodium hydroxide acid more technologically advanced and well reproducible operation compared to chromatographytandem on the sorbent.
Antimicrobial activity of individual substances and the amount of 7-guide�of axiallary and related substances of lipid nature was studied in experiments in vitro, using the method of twofold serial dilutions of substances in liquid nutrient media: mesopatamia broth - for the study of bacteriostatic activity and environment Saburo for study fungistatic activity (7).
In determining the antimicrobial spectrum of the studied samples used 6 pathogenic test microorganisms: gram-positive bacteria Staphylococcus aureus, gram-negative bacteria Escherichia coli, Proteus vulgaris and Pseudomonas bacteria as well as filamentous fungi of the genus MICROSPORUM and yeast-like fungi of the genus CANDIDA.
As a result of study set (table. 2) that the individual 7-hydroxytoluene has a high bacteriostatic activity against Staphylococcus aureus at a concentration of 15.6 μg/ml, which is about 4 times higher than the activity of individual 7-acetoxysilane. From previous research it is evident that the variation of the % content of releasenew (36÷57%) in the samples does not affect the value bacteriostatic activity against Staphylococcus aureus corresponds to 31.2 µg/ml (table. 2), while in all samples observed partial delay the growth of bacteria to a concentration of 15.6-7,8 µg/ml.
Sources of information
1. Zhen-Yan Zhu, N. Nayeshiro, R. Prewo, P. Rüedi, C. H. Eugster. Helvetica Chim Acta, v. 71, p. 577 (1988).
2. K. Grob, P. Rüedi, C. H. Eugster. Helvetica Chim Acta, v. 61, Fasc. 2, p. 871 (1978).
3. P. Rüedi. Helvetica Chim Acta, v. 69, p. 972 (1986).
4. J. M. Künde, P. Rüedi, C. H. Eugster. Helvetica Chim Acta, v. 70, p. 1911 (1987).
5. O. E. Edwards, G. Feniak, M. Los. Canadian J. of Chemistry, v. 40, p. 1540 (1962).
6. B. A. Kriwet, L. P. Tolstykh, N. And. Fedyunina. Method of quantitative determination of derivatives of oleanane (oxy - and acetoxyvalerenic) in the roots of Salvia officinalis. Chem.-Pharm. W., 1980, No. 12, pp. 66-68.
7. The report of the group of chemotherapy Department of medicine VILAR 2012
A method of producing 7-hydroxytoluene having antimicrobial activity, comprising the extraction of crushed roots of Salvia officinalis with 96% ethanol, followed by evaporation of the extract, water treatment, distillation of alcohol and a hydrophobic treatment with a solvent or extraction of the specified raw materials chloroform with subsequent treatment of the extract with water and evaporation; then the target product is extracted from the organic phase transfer in water-soluble phenolate, treating with an aqueous sodium hydroxide solution; washed with an alkaline solution of chloroform; acidified with hydrochloric or sulfuric acid; the resulting precipitate was filtered; dried and pulverized.
SUBSTANCE: method includes treating crushed Echinacea purpurea (L.) Moench roots and rhizome with steam, extraction with ethyl alcohol, then steeping, stirring, steeping, draining a portion of the extract which is equal to the amount of the loaded Echinacea purpurea (L.) Moench roots and rhizome, after draining a portion of the extract, adding ethyl alcohol to the treated material, draining the whole extract; extracting crushed Echinacea purpurea (L.) Moench herbs with ethyl alcohol, steeping, then stirring, steeping, draining a portion of the extract which is equal to the amount of the loaded Echinacea purpurea (L.) Moench herbs, after draining a portion of the extract, adding ethyl alcohol to the treated herbs, draining the whole extract; all obtained extracts are mixed, cooled and filtered under certain conditions. An Echinacea purpurea (L.) Moench tincture. Use of the method to obtain an Echinacea purpurea (L.) Moench tincture.
EFFECT: method preserves the biological activity of components of the tincture and medicinal properties.
SUBSTANCE: invention relates to a method of producing dry propolis. Said method includes grinding raw material, extraction with 96% ethyl alcohol at temperature of +20-25°C using a vacuum-ultrasonic device, filtering, purification from heavy metals and other impurities using a carbon sorbent, followed by evaporation.
EFFECT: disclosed method increases output of biologically active substances and enables purification of propolis from wax, mechanical impurities, high-molecular weight substances, heavy metals and pesticides.
2 dwg, 3 tbl, 1 ex
SUBSTANCE: means represents a dry extract of leaves and flowers of Gratiola officinalis, obtained by milling the leaves and flowers of Gratiola officinalis, extraction with 96% alcohol on a water bath to boiling and boiling, evaporation, dilution of the evaporated residue first with distilled water, then by the addition of chloroform, cooling to room temperature and centrifuging with the following separation of a water fraction and drying it under specified conditions.
EFFECT: means is non-toxic, has an expressed anti-tuberculosis action.
2 tbl, 2 ex
SUBSTANCE: method of producing α(1,2)-L-skeleton-α(1,4)-D-galactopyranosyluronan from Acorus calamus L rhizome is carried out in two steps. At the first step, crushed material is extracted with acidified water and heated on a boiling water bath while periodically mixing; after settling, the mixture is heated once more, left to cool to room temperature and filtered through a multilayer fabric filter; the filtrate is evaporated under a vacuum; the obtained solution is slowly added to 96% ethyl alcohol or to a solution of reclaimed ethanol and left in a cool place to settle the residue, after which the settled solution is drained, and the residue is filtered through a paper filter by successively washing with 96% ethyl alcohol and then with acetone. At the second step, without drying, the residue is transferred from the filter and dissolved in purified water while stirring rapidly; the obtained solution is then centrifuged; the obtained solution is purified from low-molecular weight compounds by filtering through a semi-permeable membrane; the purified solution is frozen and freeze-dried at certain conditions.
EFFECT: improved method.
3 dwg, 6 tbl
SUBSTANCE: method for producing Befungin, a preparation of Chaga mushroom involving extracting the milled raw material in heated water on a heating unit consisting of 3 reactors; adding cobalt salts to the extract in certain environment, mixing with ethanol, evaporating to dry residue of 20-25%.
EFFECT: method enables accelerating the production process, reducing energy demands, improving the quality and completeness of extraction, and improving the quality of the preparation.
2 dwg, 1 tbl, 1 ex
SUBSTANCE: invention represents the method to obtain the soluble concentrate from sideline products of reindeer antlers, comprising aqueous raw extraction, grounded up to the forced meat condition with particle size of 3-5 mm under the action of ultrasonic vibrations with frequency of 37 kHz with subsequent filtration and vacuum drying at temperature of 45°C and pressure of 0.9 atm, distinguished by the fact that the aqueous raw extraction is carried out at temperature of 35-36°C in the presence of pepsin ferment at its concentration in raw mixture: water of 0.5% during at least 3 hours, at ratio raw: water for tails 1:5, for male genital 1:4, for uteruses with embryos and amniotic fluid 1:2.
EFFECT: significant increase of final concentrate yield from sideline products of reindeer antlers.
3 ex, 2 tbl
SUBSTANCE: invention relates to method of simultaneous obtaining of two flavonoids - patuletine and its 7-O-β-D-glucopyranoside - patuletrine. Method consists in the following: milled edge petals of flower of high flavonoid sorts of tagetes patula are extracted with hexane, dried and r-extracted with chloroform, chloroform extract is concentrated, dry residue is dissolved in mixture of petroleum ether - chloroform, precipitated sediment is filtered, washed with petroleum ether and dried, obtained dry powder is dissolved in mixture chloroform - ethanol, precipitated sediment is filtered, washed with petroleum ether and dried with obtaining patuletine. Then, extraction of raw material, which remains after chloroform processing, is carried out with ethanol, alcohol extract is filtered and concentrated, after that, water residue is subjected to liquid phase extraction with ethylacetate, then, organic layer is concentrated, dry residue is dissolved in mixture chloroform - ethylacetate, precipitated sediment is filtered, washed with cooled ethylacetate, solution of hydrochloric acid in ethanol, ethanol and ethylacetate and dried, dry powder is dissolved in mixture ethylacetate-ethanol, precipitated sediment is filtered, washed with ethylacetate and dried with obtaining patuletrine.
EFFECT: method makes it possible to obtain highly pure samples of patuletine and patuletrine, as well as increase target product output.
4 dwg, 3 tbl, 4 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely to a method for preparing lappaconitine hydrobromide (versions) A method for preparing lappaconitine hydrobromide is implemented by extraction of Aconitum leucostomum root and herb or Aconitum septentrionale root and herb in methylene chloride in a continuous extraction apparatus that is followed by decontamination by means of flash chromatography (version 1), or extraction of a herbal raw material in a polar organic solvent followed by extract removal from the organic solvent (version 2), alkalinisation and extraction of the prepared extract in methylene chloride followed by decontamination of the extract by flash chromatography.
EFFECT: method for preparing lappaconitine hydrobromide provides simplifying the technological process, reducing its length and improving higher yield of the end product of officinal purity.
7 cl, 1 tbl, 9 ex
FIELD: food industry.
SUBSTANCE: method for production of Siberian cedar seeds liqueur (with hepatoprotective, antioxidant, antihypoxic, hypolipidemic effect) by way of maceration with ethyl alcohol usage; whole Siberian cedar seeds are loaded into the reactor, poured with 70% ethyl alcohol water solution; extraction is performed under preset conditions. The medicinal preparation with hepatoprotective, antioxidant, antihypoxic, hypolipidemic effect contains Siberian cedar seeds liqueur. Usage of the medicinal preparation as a hepatoprotective remedy.
EFFECT: liqueur has pronounced hepatoprotective, antioxidant, antihypoxic and hypolipidemic effect.
6 cl, 3 dwg, 8 tbl
SUBSTANCE: method for producing a pigment complex of bisnaphthazarin for preventing inflammatory diseases, involving demineralising commercial sea urchins' crusts and needles in an organic acid solution, separating organic acid salts and protein, applying pigment solution on a chromatographic column, washing the column with diluted mineral acid and distilled water, eluting the pigment complex, combining fractions containing the pigments, removing ethanol, lyophilising concentrate in the certain environment. The complex of pigments bisnaphthazarins for preventing inflammatory diseases.
EFFECT: complex of pigments prepared by the above method is effective for preventing the inflammatory diseases.
3 cl, 2 dwg, 2 tbl, 4 ex
SUBSTANCE: invention relates to a strain of the pathogen of pseudomonosis of pigs of the collection of Federal state budgetary institution "VGNKI", deposited under the name "Pseudomonas aeruginosa No.9" and the registration number "No.9-DEP", intended for production of a vaccine against pseudomonosis of pigs.
EFFECT: invention provides high immunogenic activity and the ability of production of the vaccine against pseudomonosis of pigs.
4 ex, 3 tbl
SUBSTANCE: invention refers to medicine, namely to clinical microbiology and antimicrobial chemotherapy, and concerns developing and creating new combinations providing potentiating bactericidal action and effectively inhibiting the purulent infection caused by methicillin-resistant S. aureus by using two classes of compounds possessing the essentially different mechanism of antimicrobial action.
EFFECT: developing and creating the new combinations providing potentiating bactericidal action and effectively inhibiting the purulent infection.
2 dwg, 6 tbl
SUBSTANCE: drops possessing antiviral and immunomodulatory effects characterised by the fact that they represent a 95% ethanol infusion of wild strawberry leaves and fruit specified in: red raspberry fruit, mountain ash fruit, bilberry fruit, blood-red hawthorn fruit, cinnamon rose fruit; 15-25 mg of the substance in 1 ml of the infusion.
EFFECT: drops possess pronounced antiviral and immunomodulatory effects.
15 tbl, 5 ex
SUBSTANCE: invention relates to polymer ketimine derivatives of doxycycline, which are obtained by condensation of doxycycline hydrochloride with cationic copolymers of acrylamide with 2-ammine-ethyl methacrylate (MW=16-20 kDa) with a molar ratio of copolymer/antibiotic equal to 1.1-2.0/1 in aqueous solution with pH 8.0 at 23°C. where: m=(78.0-80.0) mol %; n=(10.0-14.0) mol.%; k=(6.0-12.0) mol %.
EFFECT: polymer ketimine derivatives of doxycycline, which are non-toxic and combine the antimicrobial and immunosuppressive properties.
2 cl, 1 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to a peptide, a peptide mimetic or an amino acid derivative, which contain di-substituted β-amino acid, wherein each of substituting group in the β-amino acid, which can be identical or different, contains at least 7 non-hydrogen atoms, is lipophilic and contains at least one cyclic group; one or more cyclic groups in the substituting group can be bound or condensed with one or more numbers of cyclic groups in the other substituting groups, and when the cyclic groups are fused so that an aggregate total number of non-hydrogen atoms for these two substituting groups makes at least 12, wherein the above peptide, peptide mimetic or amino acid derivative consist of 1-4 amino acids or length-equivalent sub-units.
EFFECT: preparing the peptide, peptide mimetic or amino acid derivative, which contain the di-substituted β-amino acid.
17 cl, 4 dwg, 10 tbl, 4 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions relates to the field of pharmaceutics and medicine and deals with the application of cysteamine in the treatment of a microbial infection, caused by a microbial biofilm, as well as to a product, containing at least two anti-biofilm agents, where at least one anti-biofilm agent represents an antimicrobial peptide, and the second anti-biofilm agent represents cysteamine. Also claimed are the application of the said product in the treatment of a microbial infection and a method of preventing the formation of the microbial biofilm in a medium.
EFFECT: group of inventions provides higher antibacterial activity in comparison with the activity of any compound separately.
13 cl, 33 dwg, 2 tbl
SUBSTANCE: invention relates to organic chemistry, specifically to bensyl aralkyl ether of formula or to its pharmaceutically acceptable salt, where: Ar represents imidazolil; R1, R2, R4 and R5 independently stand for hydrogen; R3 stands for a halogen; R6 stands for trifluoromethyl or trichloromethyl; n is an integer from 0 to 2; and m is 1. The invention also relates to the use of the compound of formula (1) for the treatment and/or prevention of diseases caused by fungi, or bacteria.
EFFECT: obtained new heterocyclic compounds with useful biological properties.
4 cl, 4 dwg, 9 tbl, 8 ex
FIELD: veterinary medicine.
SUBSTANCE: method comprises intramuscular administration of the tylosin-containing preparation at a dose of 0.05 ml/kg body weight once a day for 3-4 days in all forms of mastitis. The tylosin-containing preparation is used as the preparation of the following composition, wt %: colistin sulphate - 4.0-6.0; tylosin base - 4.0-6.0; benzyl alcohol - 4.0; water for injection - 15.0; 1,2-propylene glycol - 100.0%.
EFFECT: use of the claimed invention enables to improve the therapeutic efficacy of treatment of mastitis.
3 tbl, 3 ex
SUBSTANCE: invention can be used for the local treatment of trophic ulcers, infected and persistent septic wounds, degrees I-II-IIIA burns, traumatic skin injuries, pyoinflammatory skin diseases, bed sores, etc. The invention refers to a preparation, which contains a streptocide powder 1-2.5 g, Ichthyol ointment 7-10 g and castol oil 87.5-92 ml.
EFFECT: reducing the length of treatment up to 14 days with no side effects.
SUBSTANCE: invention refers to a moulded sorbent with the antibacterial properties for treating endometritis, representing nanodispersed mesoporous carbon having a specific adsorption surface of no more than 50 m2/g and a crushing strength of not less than 20 kg/cm2, containing polyvinylpyrrolidone in an amount of not less than 5.0%, characterised by the fact that it is presented in the form of cylinders 2-4mm in diameter, 15-25mm long with one inner circular passage, to a method of treating it, as well as to a method of treating endometritis.
EFFECT: higher clinical effectiveness.
3 cl, 10 ex, 2 tbl
SUBSTANCE: group of inventions relates to a process of producing organic substrate particles bonded to switchable ferromagnetic nanoparticles with a mean particle diameter ranging from 10 to 1000 nm, to the use of such nanoparticles for hyperthermic treatment of an organism and to a drug for the hyperthermc treatment. As the ferromagnetic nanoparticles used are nanoparticles, which are non-ferromagnetic at first, but become ferromagnetic when the temperature is lowered, these initially non-ferromagnetic dispersed nanoparticles are bonded to the organic substrate particles, and then the nanoparticles bonded to the substrate particles become ferromagnetic as a result of the temperature decrease, initially the switchable ferromagnetic nanoparticles at the temperature of 22°C or higher are not ferromagnetic and become ferromagnetic as a result of cooling down to the temperatures below 22°C. The switchable ferromagnetic nanoparticle comprises Mn and additionally Fe and/or As and preferably have the Fe2P-structure or Na-Zn-13-structure, alternatively, or may comprise La, Fe and Si.
EFFECT: invention prevents the agglomeration of particles and an increase in the mean particle size.
10 cl, 1 dwg, 2 ex